CA2846905A1 - Pre-programmed non-feedback controlled continuous feeding of cell cultures - Google Patents
Pre-programmed non-feedback controlled continuous feeding of cell cultures Download PDFInfo
- Publication number
- CA2846905A1 CA2846905A1 CA2846905A CA2846905A CA2846905A1 CA 2846905 A1 CA2846905 A1 CA 2846905A1 CA 2846905 A CA2846905 A CA 2846905A CA 2846905 A CA2846905 A CA 2846905A CA 2846905 A1 CA2846905 A1 CA 2846905A1
- Authority
- CA
- Canada
- Prior art keywords
- feeding
- feed
- continuous
- cell culture
- glucose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 82
- 238000000034 method Methods 0.000 claims abstract description 102
- 230000012010 growth Effects 0.000 claims abstract description 43
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 100
- 239000008103 glucose Substances 0.000 claims description 100
- 235000015097 nutrients Nutrition 0.000 claims description 70
- 150000001413 amino acids Chemical class 0.000 claims description 15
- 210000004962 mammalian cell Anatomy 0.000 claims description 13
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 claims description 9
- 230000004044 response Effects 0.000 claims description 9
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims description 4
- 230000003213 activating effect Effects 0.000 claims description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims description 2
- 235000001014 amino acid Nutrition 0.000 claims 3
- 229940024606 amino acid Drugs 0.000 claims 3
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 claims 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims 1
- 229960001230 asparagine Drugs 0.000 claims 1
- 235000009582 asparagine Nutrition 0.000 claims 1
- 239000000758 substrate Substances 0.000 abstract description 31
- 239000000523 sample Substances 0.000 abstract description 4
- 238000012423 maintenance Methods 0.000 abstract 1
- 230000035611 feeding Effects 0.000 description 215
- 210000004027 cell Anatomy 0.000 description 124
- 238000004519 manufacturing process Methods 0.000 description 31
- 239000007787 solid Substances 0.000 description 27
- 108090000623 proteins and genes Proteins 0.000 description 25
- 102000004169 proteins and genes Human genes 0.000 description 25
- 230000008569 process Effects 0.000 description 22
- 238000013459 approach Methods 0.000 description 19
- 239000010432 diamond Substances 0.000 description 15
- 230000010261 cell growth Effects 0.000 description 14
- 238000012360 testing method Methods 0.000 description 14
- 238000002474 experimental method Methods 0.000 description 13
- 239000000203 mixture Substances 0.000 description 9
- 230000014616 translation Effects 0.000 description 9
- 230000000977 initiatory effect Effects 0.000 description 7
- 230000035899 viability Effects 0.000 description 7
- 238000009825 accumulation Methods 0.000 description 6
- 230000008901 benefit Effects 0.000 description 6
- 239000006227 byproduct Substances 0.000 description 6
- 230000003833 cell viability Effects 0.000 description 6
- 238000011161 development Methods 0.000 description 6
- 230000009977 dual effect Effects 0.000 description 6
- 238000012840 feeding operation Methods 0.000 description 6
- 238000005457 optimization Methods 0.000 description 6
- 238000012384 transportation and delivery Methods 0.000 description 6
- 238000007792 addition Methods 0.000 description 5
- 238000009795 derivation Methods 0.000 description 5
- 230000006872 improvement Effects 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 230000001186 cumulative effect Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 3
- 238000004364 calculation method Methods 0.000 description 3
- 230000008030 elimination Effects 0.000 description 3
- 238000003379 elimination reaction Methods 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 235000006286 nutrient intake Nutrition 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 230000001225 therapeutic effect Effects 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 108091006020 Fc-tagged proteins Proteins 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000012417 linear regression Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 235000021048 nutrient requirements Nutrition 0.000 description 2
- 230000003534 oscillatory effect Effects 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- NFGXHKASABOEEW-UHFFFAOYSA-N 1-methylethyl 11-methoxy-3,7,11-trimethyl-2,4-dodecadienoate Chemical compound COC(C)(C)CCCC(C)CC=CC(C)=CC(=O)OC(C)C NFGXHKASABOEEW-UHFFFAOYSA-N 0.000 description 1
- 101150067539 AMBP gene Proteins 0.000 description 1
- 101100366937 Caenorhabditis elegans sto-4 gene Proteins 0.000 description 1
- 241000905957 Channa melasoma Species 0.000 description 1
- 241000699802 Cricetulus griseus Species 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 102000004218 Insulin-Like Growth Factor I Human genes 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 101150046432 Tril gene Proteins 0.000 description 1
- 102100029469 WD repeat and HMG-box DNA-binding protein 1 Human genes 0.000 description 1
- 101710097421 WD repeat and HMG-box DNA-binding protein 1 Proteins 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 238000011021 bench scale process Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 238000004422 calculation algorithm Methods 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 150000001720 carbohydrates Chemical group 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000013400 design of experiment Methods 0.000 description 1
- 229910003460 diamond Inorganic materials 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004836 empirical method Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- CDCHDCWJMGXXRH-UHFFFAOYSA-N estazolam Chemical compound C=1C(Cl)=CC=C(N2C=NN=C2CN=2)C=1C=2C1=CC=CC=C1 CDCHDCWJMGXXRH-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000013213 extrapolation Methods 0.000 description 1
- 229920002457 flexible plastic Polymers 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 229940066779 peptones Drugs 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000011165 process development Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 229960000160 recombinant therapeutic protein Drugs 0.000 description 1
- 238000004007 reversed phase HPLC Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000003319 supportive effect Effects 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/38—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of metabolites or enzymes in the cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161535809P | 2011-09-16 | 2011-09-16 | |
| US61/535,809 | 2011-09-16 | ||
| PCT/US2012/055552 WO2013040444A1 (en) | 2011-09-16 | 2012-09-14 | Pre-programmed non-feedback controlled continuous feeding of cell cultures |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2846905A1 true CA2846905A1 (en) | 2013-03-21 |
Family
ID=46964067
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2846905A Abandoned CA2846905A1 (en) | 2011-09-16 | 2012-09-14 | Pre-programmed non-feedback controlled continuous feeding of cell cultures |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US9803166B2 (https=) |
| EP (1) | EP2756069B1 (https=) |
| JP (4) | JP2014526265A (https=) |
| KR (1) | KR101710766B1 (https=) |
| CN (1) | CN103946366B (https=) |
| AU (1) | AU2012308335A1 (https=) |
| BR (1) | BR112014008303A2 (https=) |
| CA (1) | CA2846905A1 (https=) |
| EA (1) | EA201490502A1 (https=) |
| ES (1) | ES2862576T3 (https=) |
| IL (1) | IL231032A0 (https=) |
| MX (1) | MX356956B (https=) |
| SG (1) | SG11201400656SA (https=) |
| TW (1) | TWI583788B (https=) |
| WO (1) | WO2013040444A1 (https=) |
| ZA (1) | ZA201401655B (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU765203B2 (en) | 1999-04-19 | 2003-09-11 | Shionogi & Co., Ltd. | Sulfonamide derivatives having oxadiazole rings |
| AU2015269365A1 (en) | 2014-06-04 | 2016-12-15 | Amgen Inc. | Methods for harvesting mammalian cell cultures |
| PL3865563T3 (pl) | 2014-11-25 | 2024-01-29 | Corning Incorporated | Materiały i sposoby do przedłużania pożywki do hodowli komórkowej |
| CA2969225C (en) | 2014-12-01 | 2023-08-22 | Amgen Inc. | Process for manipulating the level of glycan content of a glycoprotein |
| WO2016183016A1 (en) * | 2015-05-08 | 2016-11-17 | Flownamics Analytical Instruments, Inc. | Method & apparatus for continuous automated perfusion system harvesting from in-situ filtration probe |
| WO2021097281A1 (en) | 2019-11-15 | 2021-05-20 | Lonza Ltd | Process and system for producing an inoculum |
| US20230287335A1 (en) | 2020-07-30 | 2023-09-14 | Amgen Inc. | Cell culture media and methods of making and using the same |
| CN112553044A (zh) * | 2020-12-10 | 2021-03-26 | 上海奥浦迈生物科技股份有限公司 | 一种生物反应器细胞培养进样口用防堵塞装置 |
| KR20250056246A (ko) * | 2022-10-13 | 2025-04-25 | 가부시키가이샤 히타치세이사쿠쇼 | 배양 장치의 제어 방법, 및 세포 배양을 수반하는 배양 장치의 제어 방법 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5629202A (en) | 1994-07-19 | 1997-05-13 | Development Center For Biotechnology | Computer-controlled bioreactor system for enzymatic synthesis of L-tryptophan |
| ATE241695T1 (de) * | 1996-03-13 | 2003-06-15 | Delta Biotechnology Ltd | Gärungs-steuerungssysteme |
| WO1998041611A1 (en) | 1997-03-20 | 1998-09-24 | Regents Of The University Of Minnesota | Process for the continuous culture of cells |
| CN1678731A (zh) * | 2002-08-27 | 2005-10-05 | 范德比尔特大学 | 多室生物反应器 |
| DE102004007658B4 (de) * | 2004-02-17 | 2008-12-18 | Bioceuticals Arzneimittel Ag | Verfahren zur Herstellung von rekombinanten Proteinen in eukaryontischen Wirtszellen |
| CN1778903A (zh) | 2005-09-29 | 2006-05-31 | 华东理工大学 | 一种动物细胞高密度连续灌注培养方法 |
| WO2007071072A1 (en) * | 2005-12-22 | 2007-06-28 | Corporation De L'ecole Polytechnique De Montreal | High-rate perfusion bioreactor |
| JP2007202500A (ja) * | 2006-02-03 | 2007-08-16 | Hitachi Ltd | 培養槽の運転制御装置 |
| WO2008136398A1 (ja) | 2007-04-26 | 2008-11-13 | Chugai Seiyaku Kabushiki Kaisha | 高濃度アミノ酸含有培地を用いた細胞の培養方法 |
-
2012
- 2012-09-14 TW TW101133866A patent/TWI583788B/zh active
- 2012-09-14 MX MX2014003134A patent/MX356956B/es active IP Right Grant
- 2012-09-14 EA EA201490502A patent/EA201490502A1/ru unknown
- 2012-09-14 AU AU2012308335A patent/AU2012308335A1/en not_active Abandoned
- 2012-09-14 KR KR1020147005482A patent/KR101710766B1/ko active Active
- 2012-09-14 ES ES12766802T patent/ES2862576T3/es active Active
- 2012-09-14 JP JP2014530890A patent/JP2014526265A/ja active Pending
- 2012-09-14 SG SG11201400656SA patent/SG11201400656SA/en unknown
- 2012-09-14 BR BR112014008303A patent/BR112014008303A2/pt not_active IP Right Cessation
- 2012-09-14 EP EP12766802.8A patent/EP2756069B1/en active Active
- 2012-09-14 WO PCT/US2012/055552 patent/WO2013040444A1/en not_active Ceased
- 2012-09-14 CN CN201280044589.4A patent/CN103946366B/zh active Active
- 2012-09-14 CA CA2846905A patent/CA2846905A1/en not_active Abandoned
- 2012-09-14 US US14/342,304 patent/US9803166B2/en active Active
-
2014
- 2014-02-18 IL IL231032A patent/IL231032A0/en unknown
- 2014-03-05 ZA ZA2014/01655A patent/ZA201401655B/en unknown
-
2016
- 2016-09-30 JP JP2016193713A patent/JP2017029162A/ja active Pending
-
2019
- 2019-10-24 JP JP2019193185A patent/JP2020022492A/ja active Pending
-
2022
- 2022-01-21 JP JP2022007644A patent/JP2022058710A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| TWI583788B (zh) | 2017-05-21 |
| WO2013040444A1 (en) | 2013-03-21 |
| AU2012308335A1 (en) | 2014-03-13 |
| JP2022058710A (ja) | 2022-04-12 |
| EA201490502A1 (ru) | 2014-06-30 |
| IL231032A0 (en) | 2014-03-31 |
| MX356956B (es) | 2018-06-21 |
| CN103946366B (zh) | 2017-08-11 |
| SG11201400656SA (en) | 2014-04-28 |
| BR112014008303A2 (pt) | 2017-04-11 |
| ES2862576T3 (es) | 2021-10-07 |
| KR101710766B1 (ko) | 2017-02-27 |
| TW201326387A (zh) | 2013-07-01 |
| KR20140044396A (ko) | 2014-04-14 |
| US20140295545A1 (en) | 2014-10-02 |
| US9803166B2 (en) | 2017-10-31 |
| EP2756069A1 (en) | 2014-07-23 |
| JP2020022492A (ja) | 2020-02-13 |
| JP2014526265A (ja) | 2014-10-06 |
| EP2756069B1 (en) | 2021-01-27 |
| MX2014003134A (es) | 2014-04-25 |
| JP2017029162A (ja) | 2017-02-09 |
| ZA201401655B (en) | 2014-12-23 |
| CN103946366A (zh) | 2014-07-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2846905A1 (en) | Pre-programmed non-feedback controlled continuous feeding of cell cultures | |
| JP7391840B2 (ja) | 乳酸塩の蓄積を予防しつつ、細胞培養物を増殖させるための方法及びシステム | |
| Hsu et al. | Advanced microscale bioreactor system: a representative scale-down model for bench-top bioreactors | |
| US20200255785A1 (en) | Online biomass capacitance monitoring during large scale production of polypeptides of interest | |
| Matanguihan et al. | Upstream continuous processing: recent advances in production of biopharmaceuticals and challenges in manufacturing | |
| JP2015216886A (ja) | 細胞培養制御システム及び細胞培養制御方法 | |
| CN103080300A (zh) | 增加细胞培养物的产率和活力的二肽 | |
| Kuwae et al. | Development of a fed-batch culture process for enhanced production of recombinant human antithrombin by Chinese hamster ovary cells | |
| TW201418455A (zh) | 用於最適化灌流細胞培養系統的方法及系統 | |
| Williamson et al. | Scale‐dependent manganese leaching from stainless steel impacts terminal galactosylation in monoclonal antibodies | |
| CN109072273A (zh) | 使用全乙酰基半乳糖调节重组蛋白的蛋白质半乳糖基化概况的方法 | |
| EP2513293B1 (de) | Methode zur optimierung eines biopharmazeutischen produktionsprozesses | |
| AU2015210930A1 (en) | Perfusion media | |
| Irfan | Carbon dioxide control in bioreactors and the application of principal component analysis to cell culture process data | |
| Gupta | Implementation of CQA (critical quality attribute) based approach for development of biosimilars | |
| Nagraik | Process transfer and optimization of Chinese hamster ovary cell cultivation for monoclonal antibody production | |
| Wolf | Development and optimization of mammalian cell perfusion cultures for continuous biomanufacturing | |
| Romann | Advancing continuous biomanufacturing: Quality control and process intensification strategies for perfusion cell culture | |
| Pareek | Fed-batch bio-process development and optimization of cetuximab production at lab scale | |
| KR20110087421A (ko) | 유가 배양의 모델링을 위한 정량적 분석 방법 | |
| HK40032781A (en) | Perfusion media | |
| GRĪGS | MODEL BASED BIOMASS YIELD OPTIMIZATION AND CONTROL FOR E. coli BL21 (DE3) HEPATITIS B CORE ANTIGEN (HBcAg) PRODUCER FED-BATCH FERMENTATION PROCESS |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20140226 |
|
| FZDE | Discontinued |
Effective date: 20160829 |