TWI508772B - 微流體元件 - Google Patents
微流體元件 Download PDFInfo
- Publication number
- TWI508772B TWI508772B TW101130448A TW101130448A TWI508772B TW I508772 B TWI508772 B TW I508772B TW 101130448 A TW101130448 A TW 101130448A TW 101130448 A TW101130448 A TW 101130448A TW I508772 B TWI508772 B TW I508772B
- Authority
- TW
- Taiwan
- Prior art keywords
- reaction
- temperature
- microfluidic
- microfluidic element
- sample injection
- Prior art date
Links
- 239000012530 fluid Substances 0.000 title claims description 37
- 238000006243 chemical reaction Methods 0.000 claims description 150
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- 238000002347 injection Methods 0.000 claims description 36
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- 239000003153 chemical reaction reagent Substances 0.000 claims description 24
- 238000010438 heat treatment Methods 0.000 claims description 22
- 229910052751 metal Inorganic materials 0.000 claims description 14
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- BPUBBGLMJRNUCC-UHFFFAOYSA-N oxygen(2-);tantalum(5+) Chemical compound [O-2].[O-2].[O-2].[O-2].[O-2].[Ta+5].[Ta+5] BPUBBGLMJRNUCC-UHFFFAOYSA-N 0.000 description 15
- 229910001936 tantalum oxide Inorganic materials 0.000 description 15
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- KRHYYFGTRYWZRS-UHFFFAOYSA-N Fluorane Chemical compound F KRHYYFGTRYWZRS-UHFFFAOYSA-N 0.000 description 8
- 229910052782 aluminium Inorganic materials 0.000 description 8
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 8
- 238000006392 deoxygenation reaction Methods 0.000 description 8
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 8
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- 238000001020 plasma etching Methods 0.000 description 4
- 229910052697 platinum Inorganic materials 0.000 description 4
- WGTYBPLFGIVFAS-UHFFFAOYSA-M tetramethylammonium hydroxide Chemical compound [OH-].C[N+](C)(C)C WGTYBPLFGIVFAS-UHFFFAOYSA-M 0.000 description 4
- 238000000137 annealing Methods 0.000 description 3
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- 229910052715 tantalum Inorganic materials 0.000 description 3
- GUVRBAGPIYLISA-UHFFFAOYSA-N tantalum atom Chemical compound [Ta] GUVRBAGPIYLISA-UHFFFAOYSA-N 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
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- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- KJTLSVCANCCWHF-UHFFFAOYSA-N Ruthenium Chemical compound [Ru] KJTLSVCANCCWHF-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
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- WYTGDNHDOZPMIW-RCBQFDQVSA-N alstonine Natural products C1=CC2=C3C=CC=CC3=NC2=C2N1C[C@H]1[C@H](C)OC=C(C(=O)OC)[C@H]1C2 WYTGDNHDOZPMIW-RCBQFDQVSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000005229 chemical vapour deposition Methods 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
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- 229910052707 ruthenium Inorganic materials 0.000 description 2
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- 102000053602 DNA Human genes 0.000 description 1
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Classifications
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- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
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- B01L2300/0627—Sensor or part of a sensor is integrated
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- B01L2300/0809—Geometry, shape and general structure rectangular shaped
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- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
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- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44708—Cooling
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44791—Microapparatus
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
- Micromachines (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2011180318 | 2011-08-22 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TW201323075A TW201323075A (zh) | 2013-06-16 |
| TWI508772B true TWI508772B (zh) | 2015-11-21 |
Family
ID=47746156
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW101130448A TWI508772B (zh) | 2011-08-22 | 2012-08-22 | 微流體元件 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20140220668A1 (enExample) |
| EP (1) | EP2749349A4 (enExample) |
| JP (1) | JP5965908B2 (enExample) |
| KR (1) | KR101664201B1 (enExample) |
| CN (1) | CN103781543A (enExample) |
| IN (1) | IN2014CN01311A (enExample) |
| SG (1) | SG11201400130RA (enExample) |
| TW (1) | TWI508772B (enExample) |
| WO (1) | WO2013027393A1 (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI512261B (zh) | 2012-06-22 | 2015-12-11 | Panasonic Corp | 微流體元件 |
| KR102041205B1 (ko) | 2013-03-18 | 2019-11-06 | 주식회사 미코바이오메드 | 패턴 히터가 반복 배치된 pcr 열 블록 및 이를 포함하는 pcr 장치 |
| WO2015019522A1 (ja) | 2013-08-08 | 2015-02-12 | パナソニック株式会社 | 核酸増幅デバイス、核酸増幅装置及び核酸増幅方法 |
| EP3178919B1 (en) * | 2014-08-08 | 2021-01-06 | Panasonic Intellectual Property Management Co., Ltd. | Nucleic acid amplification device |
| CN104511258B (zh) * | 2014-12-22 | 2017-02-22 | 华中科技大学 | 施加温度偏场的交流电热微流体混合器及方法 |
| EP3269445A4 (en) | 2015-03-09 | 2018-04-11 | Panasonic Intellectual Property Management Co., Ltd. | Microfluid device |
| KR102510230B1 (ko) * | 2015-10-20 | 2023-03-15 | 주식회사 퀀타매트릭스 | 복수의 열 블록을 관통하는 반응 튜브를 포함하는 pcr 장치 |
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- 2012-08-21 EP EP12825200.4A patent/EP2749349A4/en not_active Ceased
- 2012-08-21 SG SG11201400130RA patent/SG11201400130RA/en unknown
- 2012-08-21 CN CN201280040874.9A patent/CN103781543A/zh active Pending
- 2012-08-21 US US14/240,036 patent/US20140220668A1/en not_active Abandoned
- 2012-08-21 KR KR1020147004399A patent/KR101664201B1/ko not_active Expired - Fee Related
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|---|---|
| CN103781543A (zh) | 2014-05-07 |
| JP5965908B2 (ja) | 2016-08-10 |
| WO2013027393A1 (ja) | 2013-02-28 |
| TW201323075A (zh) | 2013-06-16 |
| KR20140039079A (ko) | 2014-03-31 |
| JPWO2013027393A1 (ja) | 2015-03-05 |
| SG11201400130RA (en) | 2014-04-28 |
| EP2749349A1 (en) | 2014-07-02 |
| KR101664201B1 (ko) | 2016-10-11 |
| IN2014CN01311A (enExample) | 2015-08-28 |
| EP2749349A4 (en) | 2014-07-02 |
| US20140220668A1 (en) | 2014-08-07 |
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