TWI323284B - Amplification process - Google Patents

Info

Publication number

TWI323284B

TWI323284B TW091106126A TW91106126A TWI323284B TW I323284 B TWI323284 B TW I323284B TW 091106126 A TW091106126 A TW 091106126A TW 91106126 A TW91106126 A TW 91106126A TW I323284 B TWI323284 B TW I323284B

Authority

TW

Taiwan

Prior art keywords

pyrophosphatase

nucleic acid

reaction mixture

reaction

acid amplification

Prior art date

2001-04-30

Links

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• 238000000034 method Methods 0.000 title claims description 80
• 230000003321 amplification Effects 0.000 title claims description 39
• 238000003199 nucleic acid amplification method Methods 0.000 title claims description 39
• 230000008569 process Effects 0.000 title description 9
• 238000006243 chemical reaction Methods 0.000 claims description 108
• 108010009413 Pyrophosphatases Proteins 0.000 claims description 101
• 102000009609 Pyrophosphatases Human genes 0.000 claims description 99
• 238000003752 polymerase chain reaction Methods 0.000 claims description 88
• 239000011541 reaction mixture Substances 0.000 claims description 51
• XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 claims description 47
• 235000011180 diphosphates Nutrition 0.000 claims description 46
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• 239000000523 sample Substances 0.000 claims description 25
• 238000012937 correction Methods 0.000 claims description 24
• 238000012360 testing method Methods 0.000 claims description 24
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• 108010009595 Inorganic Pyrophosphatase Proteins 0.000 claims description 20
• 239000003153 chemical reaction reagent Substances 0.000 claims description 19
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• 239000000243 solution Substances 0.000 description 1
• 229910052717 sulfur Inorganic materials 0.000 description 1
• 239000011593 sulfur Substances 0.000 description 1
• 239000006228 supernatant Substances 0.000 description 1
• 108010061238 threonyl-glycine Proteins 0.000 description 1
• 239000001226 triphosphate Substances 0.000 description 1
• 235000011178 triphosphate Nutrition 0.000 description 1
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• 229940038773 trisodium citrate Drugs 0.000 description 1
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• 108010072695 valyl-valyl-tyrosyl-proline Proteins 0.000 description 1
• 108010009962 valyltyrosine Proteins 0.000 description 1