AR035238A1 - Un metodo para la conduccion de una reaccion de amplificacion de acido nucleico, un equipo para dicha conduccion y el uso de una enzima pirofosfatasa termoestable para llevar a cabo reacciones de amplificacion - Google Patents
Un metodo para la conduccion de una reaccion de amplificacion de acido nucleico, un equipo para dicha conduccion y el uso de una enzima pirofosfatasa termoestable para llevar a cabo reacciones de amplificacionInfo
- Publication number
- AR035238A1 AR035238A1 ARP020101570A ARP020101570A AR035238A1 AR 035238 A1 AR035238 A1 AR 035238A1 AR P020101570 A ARP020101570 A AR P020101570A AR P020101570 A ARP020101570 A AR P020101570A AR 035238 A1 AR035238 A1 AR 035238A1
- Authority
- AR
- Argentina
- Prior art keywords
- polymerase
- reaction mixture
- driving
- amplification reaction
- thermostable
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title abstract 8
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 8
- 238000006243 chemical reaction Methods 0.000 title abstract 6
- 102000009609 Pyrophosphatases Human genes 0.000 title abstract 5
- 108010009413 Pyrophosphatases Proteins 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 239000011541 reaction mixture Substances 0.000 abstract 5
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical class [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 abstract 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 2
- 241000567139 Aeropyrum pernix Species 0.000 abstract 1
- 241000205156 Pyrococcus furiosus Species 0.000 abstract 1
- 241000205188 Thermococcus Species 0.000 abstract 1
- 241000205180 Thermococcus litoralis Species 0.000 abstract 1
- 241000589596 Thermus Species 0.000 abstract 1
- 241000589500 Thermus aquaticus Species 0.000 abstract 1
- 241000557720 Thermus brockianus Species 0.000 abstract 1
- 241000589499 Thermus thermophilus Species 0.000 abstract 1
- 235000011180 diphosphates Nutrition 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/525—Phosphatase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
- C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
- C12Q2549/101—Hot start
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0110501.4A GB0110501D0 (en) | 2001-04-30 | 2001-04-30 | Amplification process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR035238A1 true AR035238A1 (es) | 2004-05-05 |
Family
ID=9913697
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP020101570A AR035238A1 (es) | 2001-04-30 | 2002-04-29 | Un metodo para la conduccion de una reaccion de amplificacion de acido nucleico, un equipo para dicha conduccion y el uso de una enzima pirofosfatasa termoestable para llevar a cabo reacciones de amplificacion |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US6951744B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP1390532B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP4177118B2 (cg-RX-API-DMAC7.html) |
| KR (2) | KR20100005245A (cg-RX-API-DMAC7.html) |
| CN (1) | CN1318604C (cg-RX-API-DMAC7.html) |
| AR (1) | AR035238A1 (cg-RX-API-DMAC7.html) |
| AT (1) | ATE502118T1 (cg-RX-API-DMAC7.html) |
| AU (1) | AU2002249444B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2445864C (cg-RX-API-DMAC7.html) |
| DE (1) | DE60239456D1 (cg-RX-API-DMAC7.html) |
| GB (2) | GB0110501D0 (cg-RX-API-DMAC7.html) |
| NZ (1) | NZ528919A (cg-RX-API-DMAC7.html) |
| TW (1) | TWI323284B (cg-RX-API-DMAC7.html) |
| WO (1) | WO2002088387A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0112868D0 (en) | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
| CN1500887A (zh) * | 2002-10-01 | 2004-06-02 | ���µ�����ҵ��ʽ���� | 引物伸长反应检测方法、碱基种类判别方法及其装置 |
| EP1634965B1 (en) | 2004-09-09 | 2010-01-20 | Roche Diagnostics GmbH | Real time PCR with the addition of pyrophosphatase |
| US20060051796A1 (en) * | 2004-09-09 | 2006-03-09 | Inga Boell | Real time PCR with the addition of pyrophosphatase |
| GB0502010D0 (en) * | 2005-02-01 | 2005-03-09 | Enigma Diagnostics Ltd | Biochemical reagents and their uses |
| CA2653841C (en) | 2006-06-01 | 2016-07-19 | Trilink Biotechnologies | Chemically modified oligonucleotide primers for nucleic acid amplification |
| KR101098764B1 (ko) * | 2007-10-29 | 2011-12-26 | (주)바이오니아 | 안정화된 핫스타트 ｐｃｒ용 건조 조성물 |
| US8133669B2 (en) | 2008-05-27 | 2012-03-13 | Trilink Biotechnologies | Chemically modified nucleoside 5′-triphosphates for thermally initiated amplification of nucleic acid |
| EP2566959A1 (en) * | 2010-04-30 | 2013-03-13 | Roche Diagniostics GmbH | System and method for purification and use of inorganic pyrophosphatase from aquifex aeolicus |
| US20110312557A1 (en) * | 2010-06-17 | 2011-12-22 | Geneasys Pty Ltd | Loc device for pathogen detection with dialysis, lysis and parallel nucleic acid amplification |
| KR101870311B1 (ko) * | 2012-03-09 | 2018-06-25 | (주)바이오니아 | 핫스타트 역전사반응 또는 핫스타트 역전사 중합효소 연쇄반응용 조성물 |
| KR102264617B1 (ko) | 2012-06-14 | 2021-06-14 | 라이프 테크놀로지스 코포레이션 | 폴리머라제 연쇄 반응 (pcr)을 위한 신규 조성물, 방법 및 키트 |
| GB201301457D0 (en) | 2013-01-28 | 2013-03-13 | Fluorogenics Ltd | Freeze-dried composition |
| JP6467829B2 (ja) * | 2014-09-03 | 2019-02-13 | 東洋紡株式会社 | 改良されたｒｔ−ｐｃｒ反応 |
| CN104862288B (zh) * | 2015-05-08 | 2019-10-29 | 苏州大学 | 一种新型温度响应性无机焦磷酸酶偶联物的合成及其在增强聚合酶链式反应中的应用 |
| LT3402880T (lt) | 2016-01-15 | 2024-03-12 | Thermo Fisher Scientific Baltics Uab | Termofiliniai dnr polimerazės mutantai |
| EP3645710A1 (en) | 2017-06-26 | 2020-05-06 | Thermo Fisher Scientific Baltics Uab | Thermophilic dna polymerase mutants |
| JP7234598B2 (ja) * | 2018-01-26 | 2023-03-08 | 東ソー株式会社 | 核酸増幅試薬および当該試薬を用いた核酸増幅制御方法 |
| CN113481180A (zh) * | 2021-07-05 | 2021-10-08 | 吉林大学 | 碱性嗜热无机焦磷酸酶及其在增强聚合酶链式反应和合成udp-半乳糖反应中的应用 |
| WO2025117716A1 (en) * | 2023-11-30 | 2025-06-05 | Watchmaker Genomics, Inc. | Taq dna polymerase hotstart antibodies and methods of use thereof |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4868103A (en) | 1986-02-19 | 1989-09-19 | Enzo Biochem, Inc. | Analyte detection by means of energy transfer |
| CH670709A5 (cg-RX-API-DMAC7.html) * | 1986-04-24 | 1989-06-30 | Univ Moskovsk | |
| US5498523A (en) * | 1988-07-12 | 1996-03-12 | President And Fellows Of Harvard College | DNA sequencing with pyrophosphatase |
| JP2997043B2 (ja) * | 1989-04-12 | 2000-01-11 | プレジデント アンド フェロウズ オブ ハーバード カレッジ | 改良されたプライマー伸長反応 |
| KR100236506B1 (ko) | 1990-11-29 | 2000-01-15 | 퍼킨-엘머시터스인스트루먼츠 | 폴리머라제 연쇄 반응 수행 장치 |
| FI923911L (fi) * | 1992-09-01 | 1994-03-02 | Vsevolod Kiselev | DNA-molekylers in vitro-syntes |
| US5565339A (en) | 1992-10-08 | 1996-10-15 | Hoffmann-La Roche Inc. | Compositions and methods for inhibiting dimerization of primers during storage of polymerase chain reaction reagents |
| US5491063A (en) | 1994-09-01 | 1996-02-13 | Hoffmann-La Roche Inc. | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| US5665551A (en) * | 1995-09-13 | 1997-09-09 | Roche Molecular Systems, Inc. | Purified nucleic acid encoding a thermostable pyrophosphatase |
| US6291164B1 (en) * | 1996-11-22 | 2001-09-18 | Invitrogen Corporation | Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate |
| GB9716052D0 (en) | 1996-12-06 | 1997-10-01 | Secr Defence | Reaction vessels |
| GB9725237D0 (en) | 1997-11-29 | 1998-01-28 | Secr Defence | Amplification system |
| GB9725197D0 (en) | 1997-11-29 | 1998-01-28 | Secr Defence | Detection system |
| GB9803382D0 (en) | 1998-02-19 | 1998-04-15 | Secr Defence | Detection system |
| US6183998B1 (en) | 1998-05-29 | 2001-02-06 | Qiagen Gmbh Max-Volmer-Strasse 4 | Method for reversible modification of thermostable enzymes |
| GB9812768D0 (en) | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
| KR100292883B1 (ko) * | 1999-02-09 | 2001-06-15 | 박한오 | 피로포스페이트와 피로포스파타제를 포함하는 핫 스타트 ｐｃｒ 반응혼합물 |
| WO2001062975A2 (en) | 2000-02-23 | 2001-08-30 | City Of Hope | Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination |
| KR20050005626A (ko) * | 2003-07-07 | 2005-01-14 | 주식회사 포스코 | 코크스 오븐에서의 장입탄 밀도 향상방법 |
-
2001
- 2001-04-30 GB GBGB0110501.4A patent/GB0110501D0/en not_active Ceased
-
2002
- 2002-03-28 TW TW091106126A patent/TWI323284B/zh not_active IP Right Cessation
- 2002-04-22 KR KR1020097025792A patent/KR20100005245A/ko not_active Withdrawn
- 2002-04-22 NZ NZ528919A patent/NZ528919A/en unknown
- 2002-04-22 JP JP2002585667A patent/JP4177118B2/ja not_active Expired - Fee Related
- 2002-04-22 GB GB0209052A patent/GB2377991B/en not_active Expired - Fee Related
- 2002-04-22 AU AU2002249444A patent/AU2002249444B2/en not_active Ceased
- 2002-04-22 WO PCT/GB2002/001861 patent/WO2002088387A2/en not_active Ceased
- 2002-04-22 AT AT02718374T patent/ATE502118T1/de not_active IP Right Cessation
- 2002-04-22 DE DE60239456T patent/DE60239456D1/de not_active Expired - Lifetime
- 2002-04-22 KR KR1020037014191A patent/KR101039563B1/ko not_active Expired - Fee Related
- 2002-04-22 EP EP02718374A patent/EP1390532B1/en not_active Expired - Lifetime
- 2002-04-22 CA CA2445864A patent/CA2445864C/en not_active Expired - Fee Related
- 2002-04-22 CN CNB028132726A patent/CN1318604C/zh not_active Expired - Fee Related
- 2002-04-29 AR ARP020101570A patent/AR035238A1/es unknown
- 2002-04-30 US US10/135,807 patent/US6951744B2/en not_active Expired - Fee Related
-
2005
- 2005-08-17 US US11/205,667 patent/US7449312B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2445864C (en) | 2011-08-02 |
| JP4177118B2 (ja) | 2008-11-05 |
| GB0110501D0 (en) | 2001-06-20 |
| CN1318604C (zh) | 2007-05-30 |
| EP1390532B1 (en) | 2011-03-16 |
| GB0209052D0 (en) | 2002-05-29 |
| WO2002088387A2 (en) | 2002-11-07 |
| GB2377991B (en) | 2004-01-28 |
| EP1390532A2 (en) | 2004-02-25 |
| US6951744B2 (en) | 2005-10-04 |
| KR20040015216A (ko) | 2004-02-18 |
| AU2002249444B2 (en) | 2007-07-12 |
| KR20100005245A (ko) | 2010-01-14 |
| CN1522305A (zh) | 2004-08-18 |
| ATE502118T1 (de) | 2011-04-15 |
| KR101039563B1 (ko) | 2011-06-09 |
| US20060057617A1 (en) | 2006-03-16 |
| JP2004524853A (ja) | 2004-08-19 |
| NZ528919A (en) | 2005-07-29 |
| US20030049655A1 (en) | 2003-03-13 |
| DE60239456D1 (de) | 2011-04-28 |
| GB2377991A (en) | 2003-01-29 |
| TWI323284B (en) | 2010-04-11 |
| CA2445864A1 (en) | 2002-11-07 |
| WO2002088387A3 (en) | 2003-12-11 |
| US7449312B2 (en) | 2008-11-11 |
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| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |