CA2445864C - A hot start amplification process using pyrophosphatase enzyme - Google Patents

Info

Publication number

CA2445864C

CA2445864C CA2445864A CA2445864A CA2445864C CA 2445864 C CA2445864 C CA 2445864C CA 2445864 A CA2445864 A CA 2445864A CA 2445864 A CA2445864 A CA 2445864A CA 2445864 C CA2445864 C CA 2445864C

Authority

CA

Canada

Prior art keywords

ppase

lys

val

glu

polymerase

Prior art date

2001-04-30

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Expired - Fee Related

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• 238000000034 method Methods 0.000 title claims abstract description 80
• 230000003321 amplification Effects 0.000 title claims abstract description 56
• 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 56
• 108010009413 Pyrophosphatases Proteins 0.000 title claims abstract description 28
• 102000009609 Pyrophosphatases Human genes 0.000 title claims abstract description 27
• 230000008569 process Effects 0.000 title description 8
• 238000006243 chemical reaction Methods 0.000 claims abstract description 72
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• 239000011541 reaction mixture Substances 0.000 claims abstract description 38
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• 108020004707 nucleic acids Proteins 0.000 claims abstract description 11
• 238000003752 polymerase chain reaction Methods 0.000 claims description 76
• 235000011180 diphosphates Nutrition 0.000 claims description 35
• 108010006785 Taq Polymerase Proteins 0.000 claims description 33
• 102000004190 Enzymes Human genes 0.000 claims description 28
• 108090000790 Enzymes Proteins 0.000 claims description 28
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 27
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 27
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• 239000000523 sample Substances 0.000 claims description 22
• 239000003153 chemical reaction reagent Substances 0.000 claims description 19
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