TW201904593A - Cosmetic and skin protecting agent containing lactic acid bacterium - Google Patents

Abstract

The present invention addresses the problem of providing a cosmetic and a skin protecting agent by which the balance of skin indigenous bacteria can be improved and, preferably, the ratio of Staphylococcus epidermidis, which is a useful bacterium, can be increased. This problem can be solved by providing an agent for improving the balance of skin indigenous bacteria, said agent comprising as an active ingredient Lactobacillus paracasei MCC1849 (NITE BP-01633), etc.

Description

 Skin resident bacteria balance improver, cosmetic material containing lactic acid bacteria, and skin protectant  

The present invention relates to a cosmetic material which protects the skin from external stress or harmful bacteria by improving the ratio of the resident bacteria to the bacteria, that is, increasing the ratio of Staphylococcus epidermidis belonging to the useful bacteria. And a skin protectant.

The human system maintains the living environment together with various symbiotic microorganisms. Representative of commensal microorganisms are intestinal bacteria. In the intestinal bacteria, lactic acid bacteria are known as useful bacteria. It has been reported that the function of the lactic acid bacteria is not limited to the adjustment of the intestinal tract, but also the production of useful components such as organic acids by digesting dietary fibers and the like, and improving the function of the living body. Also plays a role. Furthermore, it is also reported that polysaccharides such as phosphatidylcholine acid (Lipoteichoic acid) promote the formation of intestinal barriers, and can also prevent and improve inflammatory bowel diseases such as colitis. Intestinal bacteria also contain harmful toxins such as Clostridium perfringens or Escherichia coli, but these harmful bacteria are also not suitable for the maintenance of anti-inflammatory immunity of cells. In addition, in recent years, it has become more and more important to improve the balance between the useful bacteria and the harmful bacteria in comparison with the component for the purpose of sterilization of harmful bacteria (Non-Patent Document 1).

In the intestine, in order to improve the intestinal environment, a useful bacterium such as lactic acid bacteria or bifidobacteria is taken, but in order to keep the useful bacteria in a state of survival and reach the intestine and colonize, it is necessary to select an acid-resistant strain or an enteric-soluble capsule. Wait. Further, in order to increase the useful bacteria in the intestine, the following method is also employed, that is, a food or drink in which various oligosaccharides which are prepared by the dietary fiber or selectively digested by the useful bacteria are ingested. However, depending on the individual's constitution, these methods sometimes cause excessive intestinal fermentation or diarrhea. As a method for solving such a problem, a method of heat-treating dead cells using Lactobacillus is also being studied.

It is known that heat-treated dead cells of Lactobacillus paracasei, which is a kind of lactic acid bacteria which are useful as intestinal bacteria, can induce the production of interleukin-12 (IL-12) as an anti-inflammatory cytokine. Improve intestinal function. In addition, the dead cells are also easily processed into foods and drinks, and there is little influence on the storage stability of foods and drinks without excessive fermentation.

It is known that, as in the intestine, the resident symbiotic microorganisms also form a community on the skin. The effects of these commensal microorganisms on the skin are similar to those of the intestinal bacteria in the intestines. The community of symbiotic microorganisms of the skin is called a skin bacterium, and is mainly composed of Staphylococcus epidermidis, Staphylococcus aureus, Propionibacterium acnes, Corynebacterium, and the like. These skin-sustaining bacteria usually maintain a certain ratio of balance in order to maintain the protective function of protecting the skin from external influences.

As an example of the balance damage, the ratio of Staphylococcus aureus in the skin of a patient with atopic dermatitis is increased. Regarding this patient, it is also known that Staphylococcus aureus produces toxins, causing further deterioration of the skin condition.

For healthy people, it is also known that due to environmental factors, rapid temperature degeneration, dryness, strong detergents or medicines, physical and mental factors, trauma, mental stress or disease, resulting in poor general condition The balance of the flora is destroyed, and the related protective function is lowered (Non-Patent Document 2).

A useful method for adjusting the balance of skin resident bacteria is to increase the ratio of Staphylococcus epidermidis. Staphylococcus epidermidis exerts the following physical protective functions: decomposes sebum to produce glycerin and organic acids, prevents dry skin, regulates the pH of the skin surface, and inhibits the growth of bacteria. Therefore, if the ratio of Staphylococcus epidermidis is reduced, there is a case where the protective function does not exert and the skin disease is caused. In order to prevent this skin disease, a moisturizing agent, an antibacterial agent, a bactericidal ingredient, and the like are widely used for external preparations for skin, but there is a concern that these pharmaceutical ingredients may also inhibit the protective function of Staphylococcus epidermidis.

Further, Staphylococcus epidermidis also exerts the following effects: stimulating skin epidermal keratinocytes, promoting the production of antibacterial peptides from skin epidermal keratinocytes, and enhancing the biological protective function of the living body. As an antibacterial peptide produced by skin epidermal keratinocytes, human β-defensin-3 has been known, and it has been reported that these antibacterial peptides have been shown to be Staphylococcus aureus which is a harmful microorganism of the skin. The antibacterial action acts as a biological barrier to protect the skin from harmful bacteria (Non-Patent Document 3).

In order to promote the proliferation of S. epidermidis, saccharides such as oligosaccharides have been used as a source of digestion (Patent Document 1), but not only for useful S. epidermidis, but also for other harmful bacteria such as Staphylococcus aureus. It shows digestibility, and sometimes it does not achieve skin protection. In addition, when the saccharide is applied to the skin, it tends to be sticky, and it also causes other problems such as difficulty in formulating the touch.

 [Previous Technical Literature]    [Patent Literature]  

Patent Document 1: Japanese Patent Laid-Open Publication No. 2007-077121.

 [Non-patent literature]  

Non-Patent Document 1: Yamura Minzhi, Effect of Intestinal Flora on the Immune System, Medical Sciences, Synergy Co., Ltd., P107-112 (2009).

Non-Patent Document 2: Gallo, R. L., et al., Journal of Investigative Dermatology 131, P1974-1980 (2011).

Non-Patent Document 3: Kisich, K.O., et al., Journal of Investigative Dermatology 127: 2368-2380 (2007).

The object to be solved by the invention is to provide a cosmetic material, a skin protectant, etc., which can improve the balance of the skin resident bacteria, and preferably increase the ratio of Staphylococcus epidermidis belonging to useful bacteria.

The inventors of the present invention investigated various lactic acid bacteria and repeatedly studied them. As a result, it was found that the heat-treated dead cells of Lactobacillus paracasei MCC1849 as a Lactobacillus have an effect of promoting the proliferation of Staphylococcus epidermidis, and inhibiting Staphylococcus aureus and Acne. The effect of the growth of acid bacilli. Further, it has been found to have an effect of promoting the production of glycerol derived from Staphylococcus epidermidis and an effect of promoting the production of antimicrobial peptide derived from epidermal keratinocytes. It has been found that skin balance can be improved by any one of these effects or a series of functions. Further, it has been found that, together with the heat-treated dead cells of Lactobacillus paracasei MCC1849, the polysaccharides derived from Fira aloe, derived from tamarind, derived from gum bean seeds, and the like are used together as a plant polysaccharide. The polysaccharides derived from microorganisms, such as sinensis and lanolin, multiply the proliferation effect of S. epidermidis.

The present invention provides a skin resident bacteria balance improving agent which is based on Lactobacillus paracasei MCC1849 (NITE BP-01633) as an active ingredient.

Further, the present invention provides a skin protectant comprising Lactobacillus paracasei MCC1849 (NITE BP-01633) as an active ingredient.

The skin protectant is a Staphylococcus epidermidis proliferation promoter, a Staphylococcus aureus proliferation inhibitor, a Propionibacterium acnes proliferation inhibitor, a glycerol production promoter of Staphylococcus epidermidis, or an antimicrobial peptide production promoter by epidermal cells. The preferred form.

Further, the present invention provides a cosmetic material comprising Lactobacillus paracasei MCC1849 (NITE BP-01633).

The other forms of the skin resident bacteria balance improving agent, the skin protecting agent, and the cosmetic material further include a plant polysaccharide and/or a polysaccharide derived from a microorganism as an active ingredient.

The cosmetic material and the skin protectant of the present invention can promote the proliferation of Staphylococcus epidermidis, and inhibit the proliferation of Staphylococcus aureus and Propionibacterium acnes, and adjust the balance of skin resident bacteria. Further, in a preferred embodiment, the cosmetic material and the skin protectant of the present invention can promote the production of glycerin by Staphylococcus epidermidis, prevent dry skin, adjust the pH of the skin surface, inhibit the growth of the bacteria, and promote the skin epidermal keratinocytes. Human β-defensin-3 is produced as an antibacterial peptide. The cosmetic or skin protectant of the present invention can protect the skin from external pressure or harmful bacteria such as drying through the function of any one or more of these effects.

Fig. 1 shows the results of adding the heat-treated dead bacteria of Lactobacillus paracasei MCC1849 to Staphylococcus epidermidis standard strain (ATCC12228) and measuring the number of colony formation by the culture method.

Fig. 2 shows the results of adding the heat-treated dead cells of Lactobacillus paracasei MCC1849 to Staphylococcus aureus standard strain (ATCC 6538) and measuring the number of colony formation by a culture method.

Fig. 3 shows the results of adding the heat-treated dead bacteria of Lactobacillus paracasei MCC1849 to the culture solution of the skin resident bacteria collected from the subject (between human eyebrows), and measuring the number of colony formation of Staphylococcus epidermidis.

Fig. 4 shows the results of adding the heat-treated dead bacteria of Lactobacillus paracasei MCC1849 to the culture solution of the skin resident bacteria collected from the subject (between human eyebrows), and measuring the number of colonies formed by Propionibacterium acnes.

Fig. 5 is a view showing that the heat-treated dead cells of Lactobacillus paracasei MCC1849 are added to a culture solution of a skin resident bacteria collected from a subject (human eyebrow), and the number of colonies of Staphylococcus epidermidis and P. acnes is determined. The result of the ratio of the number of colony formations was calculated.

Fig. 6 shows the results of adding the heat-treated dead cells of the genus Lactobacillus to the Staphylococcus epidermidis standard strain (ATCC12228) and measuring the number of colony formation by the culture method.

Fig. 7 shows the results of adding the heat-treated dead bacteria of Lactobacillus paracasei MCC1849 to the culture solution of Staphylococcus epidermidis standard strain (ATCC12228) and quantifying the amount of glycerol.

Fig. 8 shows the results of adding the heat-treated dead cells of the genus Lactobacillus to the culture solution of the standard strain of Staphylococcus epidermidis (ATCC12228) and quantifying the amount of glycerol.

Fig. 9 shows that a human normal keratinocyte culture strain was cultured for 24 hours in a medium containing heat-treated dead bacteria containing Lactobacillus paracasei MCC1849, and the culture supernatant was measured by ELISA (Enzyme Linked Immunosorbent Assay; enzyme-binding immunosorbent assay). The result of the amount of β-defensin-3 (hBD3) in humans.

Fig. 10 shows a culture of human normal keratinocytes cultured in a medium containing heat-treated dead cells of the genus Lactobacillus for 24 hours, and the amount of human β-defensin-3 (hBD3) in the culture supernatant was measured by ELISA. the result of.

Figure 11 shows the addition of a sample of the heat treated dead cells of Fira aloe polysaccharide, tamarind gum, sinensis gum, yam gum, cationized guar gum and Lactobacillus paracasei MCC1849 to the Staphylococcus epidermidis standard. The result of the number of colony formation was determined by the culture method in the strain (ATCC12228).

Figure 12 shows the addition of a sample of heat-treated dead cells of Fira aloe polysaccharide, tamarind gum, sambag, yam, cationized guar and Lactobacillus paracasei ATCCBBA-52 to epidermal grapes. In the standard bacteria of the cocci (ATCC 12228), the number of colony formation was determined by the culture method.

The cosmetic material or the skin resident bacteria balance improving agent, the Staphylococcus epidermidis proliferation promoting agent, the Staphylococcus aureus proliferation inhibitor, the P. acnes proliferation inhibitor, the glycerol production promoter of Staphylococcus epidermidis, and the epidermal cells Each skin protectant such as an antimicrobial peptide production promoter (in the present specification, these may be referred to as "cosmetic materials or skin protectants", "cosmetic materials and skin protectants", "cosmetic materials, skin protectants", and the like. ) contains Lactobacillus paracasei MCC1849 as an active ingredient. Regarding the Lactobacillus paracasei MCC1849 lactobacillus, it is particularly preferable to heat-treat the dead cells.

Further, the skin protectant of the present invention contains Lactobacillus paracasei MCC1849 as an active ingredient, and may contain other components. That is, the skin protectant of the present invention is equivalent to the skin protective composition. Therefore, the skin protectant of the present invention can be referred to as a composition for skin protection. Additionally, the composition is a concept comprising a mixture, whether the composition of the composition is uniform or non-uniform. In this case, the skin resident bacteria balance improving agent, the Staphylococcus epidermal growth promoting agent, the Staphylococcus aureus proliferation inhibitor, the P. acnes proliferation inhibitor, the glycerol production promoter of Staphylococcus epidermidis, and the antibacterial agent caused by epidermal cells The same applies to the peptide production promoter and the like.

The cosmetic or skin protectant of the present invention improves the balance of skin resident bacteria. The so-called skin resident bacteria balance refers to the balance between Staphylococcus epidermidis and harmful bacteria in the skin. Examples of the harmful bacteria include Staphylococcus aureus and Propionibacterium acnes. Further, the term "improving the balance of skin resident bacteria" means increasing the ratio of the number of bacteria of S. epidermidis to the number of bacteria of harmful bacteria. The improvement in the balance of the skin resident bacteria is preferably the proliferation of Staphylococcus epidermidis, the inhibition of harmful bacteria, or both.

Further, the cosmetic or skin protectant of the present invention preferably has the following characteristics.

1. Promote the production of glycerol by Staphylococcus epidermidis, thereby preventing dry skin and regulating the pH of the skin surface to inhibit the growth of bacteria.

2. Promote the epidermal keratinocytes to produce antibacterial peptides against harmful bacteria such as Staphylococcus aureus.

Further, a polysaccharide derived from a plant-derived polysaccharide such as Fira aloe, a polysaccharide derived from seaweed, a polysaccharide derived from a microorganism, and a polysaccharide such as Lactobacillus paracasei MCC1849 are used in combination, and are multiplied Promote the proliferation of Staphylococcus epidermidis.

The cosmetic or skin protectant of the present invention preferably has a function of enhancing the protection of the skin from external pressure or harmful bacteria such as drying through one or more of the above-described series of functions.

As described above, the cosmetic materials and skin protectants of the present invention can improve the balance of skin resident bacteria. The effect attached to the improvement of the balance of the skin resident bacteria or the effect of deriving the action is presumed as follows.

When the composition containing Lactobacillus paracasei MCC1849 is applied to the skin, the bacteria promote the proliferation of Staphylococcus epidermidis. Staphylococcus epidermidis produces glycerol and short-chain fatty acids that promote the production of antimicrobial peptides in skin cells. In addition, Lactobacillus paracasei MCC1849 directly acts on skin cells to promote the production of antimicrobial peptides by skin cells. Glycerin enhances the moisture retention of the skin. Short-chain fatty acids direct the skin to weak acidity, promote the proliferation of Staphylococcus epidermidis, and inhibit the proliferation of harmful bacteria such as Staphylococcus aureus or Propionibacterium acnes. It can reduce the damage of the skin barrier caused by harmful bacteria and inhibit the skin's moisture retention. Therefore, the cosmetic material and the skin protectant of the present invention can also be used for the promotion of proliferation of harmful bacteria such as Staphylococcus epidermidis, Staphylococcus aureus or Propionibacterium acnes, glycerol and/or short-chain fatty acids of Staphylococcus epidermidis. It is used for any one or two or more of the use of the antibacterial peptide for promoting the production of skin cells, and the moisturizing of the skin. However, the above effects are presumed to be in addition to the effects shown by the examples, and are not intended to limit the improvement of the skin-to-bacteria balance of the cosmetic materials and skin protectants of the present invention to those based on the above-mentioned effects.

Further, the present invention has, for example, moisturizing properties, and thus, for example, it is thought that it can be used in mammals for the prevention or treatment of diseases or symptoms which can be prevented or treated by moisturizing. Improvements are also included in "Treatment". Specific examples of the application of the present invention include a dry disease, atopic dermatitis (eczema), dry dermatitis, and dryness. Examples of the mammal include human, cow, horse, sheep, goat, pig, monkey, dog, cat, rat, mouse, hamster, guinea pig, and the like.

As a method of culturing Lactobacillus paracasei MCC1849, a method generally used for culturing lactic acid bacteria including a culture method of a conventionally known lactic acid bacterium can be appropriately modified and used as needed. For example, the culture temperature may be from 25 ° C to 50 ° C, preferably from 35 ° C to 42 ° C. Further, the culture can be carried out under both aerobic conditions and anaerobic conditions, preferably under anaerobic conditions, for example, by ventilating an anaerobic gas such as carbon dioxide. Alternatively, it may be cultured under microaerobic conditions such as liquid static culture.

The medium for culturing the Lactobacillus paracasei MCC1849 is not particularly limited, and a medium which is usually used for culturing lactic acid bacteria can be appropriately modified and used as needed. That is, as the carbon source, for example, saccharides such as galactose, glucose, fructose, mannose, cellobiose, maltose, lactose, sucrose, trehalose, starch, starch hydrolyzate, and waste molasses can be used depending on the digestibility. As the nitrogen source, for example, an ammonium salt such as ammonia, ammonium sulfate, ammonium chloride or ammonium nitrate or a nitrate can be used. Further, as the inorganic salt, for example, sodium chloride, potassium chloride, potassium phosphate, magnesium sulfate, calcium chloride, calcium nitrate, manganese chloride, ferrous sulfate or the like can be used. In addition, organic components such as peptone, soy flour, defatted soybean meal, meat extract, and yeast extract can also be used. Further, as the prepared medium, for example, MRS (de Man Rogosa Sharpe) medium can be preferably used.

The heat-treated dead cells of Lactobacillus paracasei MCC1849 can be produced by the following method. For example, the cells are recovered from the culture obtained by culturing the Lactobacillus paracasei MCC1849 in accordance with a conventional method by filtration, centrifugation, or the like, washed with water, and then suspended in water or the like for heat treatment. Further, the above-mentioned heat-treated dead cells can be prepared by concentrating and drying as needed. It can also be carried out using a spray drying, a freeze dryer or the like which is usually used. Depending on the case, the enzyme treatment, the surfactant treatment, the grinding, and the pulverization treatment may be performed before or after the sterilization treatment by heating or the like, and the treatment proceeds are also included in the present invention. In the dead cells of the invention.

The dead cells of Lactobacillus paracasei MCC1849 of the present invention are not limited to heat treatment of dead cells. The dead cells of Lactobacillus paracasei MCC1849 of the present invention may be those which cannot proliferate even when cultured under generally preferred culture conditions, and which do not exhibit metabolic activity, and include the following states. Damaged bacteria: It is difficult to proliferate even when cultured under generally preferred culture conditions, but the metabolic activity of the cells is reduced if compared with live bacteria, but if it does not show metabolic activity The cells were significantly more active than the cells. Examples of the dead cells of Lactobacillus paracasei MCC1849 of the present invention include death by chemical treatment (chemical liquid sterilization method, ethylene oxide gas sterilization method, hydrogen peroxide gas plasma sterilization method, etc.). The bacteria, or by physical treatment (flame sterilization, dry heat sterilization, ultra-high temperature heat treatment, high-pressure steam sterilization, high-frequency sterilization, radiation sterilization (gamma sterilization, X-ray sterilization, etc.), Dead cells produced by electron beam sterilization, ultraviolet sterilization, filtration sterilization, ultrasonic disruption, pressurized crushing, etc.

The water used for washing and suspending includes, in addition to pure water, tap water, well water, mineral water, mineral water, hot spring water, water inrush, fresh water, etc., including various treatments. The treatment for water includes, for example, purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, the water usable in the present invention also includes purified water, heat-treated water, ion-exchanged water, physiological saline, various buffers, and the like.

The heat-treated dead bacteria suspension of Lactobacillus paracasei MCC1849 obtained as above can be used as a skin permanent bacteria balance improving agent, a Staphylococcus epidermidis proliferation promoter, a Staphylococcus aureus proliferation inhibitor, and a Propionibacterium acnes proliferation. Each skin protectant such as an inhibitor, a glycerol production promoter of Staphylococcus epidermidis, an antimicrobial peptide production promoter by epidermal cells, or the health of maintaining the surface state of the skin via any one or a series of functions of the above Cosmetic material for skin protection.

The skin resident bacteria balance improving agent, the Staphylococcus epidermidis proliferation promoting agent, the Staphylococcus aureus proliferation inhibitor, the P. acnes proliferation inhibitor, the glycerol production promoter of the epidermidococcus, and the antibacterial agent caused by the epidermal cells of the present invention The peptide production promoter is preferably prepared by suspending the heat-treated dead cells in the range of 1 × 10 ⁶ cells/g to 1 × 10 ¹² cells/g. Further, it is further preferably prepared by suspending the heat-treated dead cells in the range of 1 × 10 ⁷ cells/g to 1 × 10 ¹¹ cells/g. In addition, as for the cosmetic material for daily use, it is generally used in a total amount of about 0.5 g, preferably from 1 × 10 ⁶ cells/g to 1 × 10 ¹² cells/g, per use. It is prepared by mixing the above-mentioned heat-treated dead cells in a range from one to one-fold, more preferably from 1×10 ⁷ cells/g to 1×10 ¹¹ cells/g. The preparation is carried out in such a manner that the above-mentioned heat-treated dead cells are contained in a range from the multiple to the first multiple. Further, in the case of the dead cells of the examples, 1 μg corresponds to 3.75 × 10 ⁶ cells, and therefore, the value can be converted.

Further, the Lactobacillus paracasei MCC1849 can be used as it is, or can be used after dilution or concentration, or can be recovered from the culture. In addition, as shown in the examples, the heat-treated dead cells of Lactobacillus paracasei MCC1849 have the effect of improving the balance of skin bacteria, promoting the growth of Staphylococcus epidermidis, inhibiting the growth of Staphylococcus aureus, and inhibiting the proliferation of Propionibacterium acnes. Skin protection effects such as the promotion of glycerol production by Staphylococcus epidermidis and the promotion of antimicrobial peptide production by epidermal cells. Therefore, it is thought that the aforementioned skin protection effect is similarly exhibited even for living bacteria. That is, in the present invention, the cells of the Lactobacillus paracasei MCC1849 may be live bacteria or dead bacteria, or may be both live bacteria and dead bacteria. The dead bacteria may also be a broken matter of dead cells or an isolated isolate of dead bacteria having the aforementioned effects.

In the present invention, in the case of using live bacteria of Lactobacillus paracasei MCC1849, it is preferably from 1 × 10 ⁶ CFU/g to 1 × 10 ¹² CFU/g or 1 × 10 ⁶ CFU/mL to 1 ×. Suspension preparation was carried out in the form of 10 ¹² CFU/mL (CFU: Colony Forming Unit) containing live bacteria. Further, it is further preferably prepared by suspending the living bacteria in a range of from 1 × 10 ⁷ CFU/g to 1 × 10 ¹¹ CFU/g or from 1 × 10 ⁷ CFU/g to 1 × 10 ¹¹ CFU/mL. In the case of dead bacteria, cfu/g or cfu/ml can be replaced with cells/g or cells/ml.

(plant polysaccharides, polysaccharides derived from seaweed, polysaccharides derived from microorganisms)

In the cosmetic or skin protectant of the present invention, together with Lactobacillus paracasei MCC1849, a group selected from the group consisting of plant polysaccharides, polysaccharides derived from seaweed, and polysaccharides derived from microorganisms may also be used. More than one. Examples of the plant polysaccharides include polysaccharides belonging to plants of the genus Aloe. As aloe plants, Aloe barbadensis Miller, Aloe arborescen Miller var. natalensis Berger, Aloe ferox Miller, Aloe africana Miller, Aloe spicata Baker) and so on.

The plant polysaccharide to be used in the cosmetic or skin protectant of the present invention is preferably an isolate using a polysaccharide containing the aforementioned plant, and can be used in the form of an isolate obtained by separating the whole plant as described above, preferably It is used in the form of an isolate obtained by separating mesophyll (transparent gel fraction). The isolate obtained by the following method can be used as the plant polysaccharide used in the present invention: it is preferred to crush the plant or a part thereof using a homogenizer or the like, and if necessary, press and filter, thereby preparing It is liquid and is extracted, washed, etc. with an organic solvent or hot water.

Further, the plant-containing polysaccharide-containing isolate used in the present invention may contain other components contained in the plant in addition to the polysaccharide derived from the plant, without affecting the effects of the present invention. For example, in the case of a plant belonging to the genus Aloe, sterols, steroids and the like can be exemplified in addition to the polysaccharides contained in the plants belonging to the genus Aloe.

Further, examples of the polysaccharide derived from plants include tamarind gum and cationized guar gum. Examples of the polysaccharide derived from seaweed include alginic acid, fucodyne, ascophyllan, and porphyran. Further, examples of the polysaccharide derived from microorganisms include three-scented gum and nodular gum.

The skin resident bacteria balance improving agent, the Staphylococcus epidermidis proliferation promoting agent, the Staphylococcus aureus proliferation inhibitor, the P. acnes proliferation inhibitor, the glycerol production promoter of Staphylococcus epidermidis, and the antimicrobial peptide derived from epidermal cells of the present invention In the production accelerator, the polysaccharide is preferably added in such a manner that the solid content of the polysaccharide is contained in a range of 0.001 g to 0.5 g per gram, more preferably 0.01 g to 0.1 per gram. The above polysaccharide is added in such a manner that the solid content component of the above polysaccharide is contained in the range of g. In addition, as for the cosmetic material for daily use, it is generally used in a total amount of about 0.5 g per one use, preferably one to one-fold to one-fold from 0.001 g to 0.5 g per gram. The above polysaccharide is added in such a manner that the solid content component of the polysaccharide is contained in the amount range, and it is more preferable to contain the above-mentioned polysaccharide in a range of from one to one-fold to one-fold by weight of 0.01 g to 0.1 g per gram. The above polysaccharide is added as a solid component of the saccharide.

(Forms of cosmetic materials and skin protectants)

The cosmetic material and the skin protectant of the present invention can be made into a form suitable for use in ampoules, capsules, powders, granules, liquids, gels, bubbles, emulsions, sheets, mists, sprays and the like. The cosmetic material and the skin protectant of the present invention can be used as an external preparation, and the form and classification of the external preparation are not limited as long as they are applied to the skin. For example, 1) external pharmaceuticals; 2) topical quasi-drugs; 3) cosmetic materials (for example, lotions, lotions, creams, ointments, lotion, body rinse, facial milk) (facial rinse), oil, smear mask, mask, makeup oil, cosmetic oil and other basic makeup materials, facial cleanser, skin cleansing lotion, scrub, massage agent, cleaning agent, makeup remover , oil absorbing agent, depilatory agent, depilatory agent, shaving agent, after shave lotion, pre-shave lotion, shaving cream, shaving lotion, tanning oil, suntan lotion, tanning cream, sunscreen, suntan lotion, Sunscreen lotion, whitening agent, body powder, talcum powder, bath powder, perfume powder, foundation, powder, powder, make-up powder, make-up cream , make-up base, lipstick, lipstick, lip glaze, rouge, blush, eye shadow, eyeliner, mascara, eyebrow pencil, false eyelash makeup, mascara, eye make-up, eye make-up remover Make-up makeup materials, cologne (eau de cologne), cologne (perfume cologne), eau de toilette , ordinary perfume, powder perfume, solid balm, perfume, fragrance, nail cream, nail polish, nail polish, nail polish remover, nail polish remover, anti-mite, anti-body odor, deodorant, deodorant Agent, antiperspirant, antiperspirant, paste, ointment, patch, tablet, patch, aerosol, etc.); 4) for scalp, hair medicinal or / and cosmetic preparations ( For example, shampoo, conditioner, hair care agent, hair pre-care agent, scalp care agent, scalp pre-care agent, perm liquid, hair oil, glazed oil, hair oil, hair oil, styling milk, styling mousse Hair blow, hair brushing, chic, hair cream, pomade, hair spray, hair liquid, hair styling, hair dye, hair dye, bleaching agent, hair extension Agent, hair growth agent, hair growth agent, paste, ointment, tape, tablet, patch, aerosol, etc.); 5) bath agent used in bath water; 6) other than sanitary products , sanitary napkins, wet wipes, disposable diapers, first aid plasters, cooling gel sheets, etc.

The cosmetic material and the skin protectant of the present invention can be applied to the skin by application, attachment, contact, or the like according to the form thereof. In the case of a bathing agent, it can be applied to the skin by immersing the skin in a bath water or the like to which a bathing agent is added.

Further, the cosmetic material and the skin protectant of the present invention may be local or whole body in the application site of the skin. In the case of a local condition, it may be any part of the whole body such as the head, face, head, wrist, abdomen, back, buttocks, feet, feet, and the like.

Further, the cosmetic material and the skin protectant of the present invention may be applied to the skin one or more times a day, or may be once every several days.

(can be used for other constituents of cosmetic materials)

Such a cosmetic material can be arbitrarily selected and used as exemplified by the components or additives exemplified below, without affecting the effects of the present invention, and the amount of the components or additives added to the formulation system is not particularly limited. The regulation is generally considered to be about 0.0001% to 50%.

(1) Various fats and oils

Avocado oil, almond oil, fennel oil, perilla oil, olive oil, orange oil, orange roughy oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cape oil, butter Fatty acid, macadamia oil, safflower oil, shea butter, liquid shea butter, soybean oil, camellia oil, corn oil, rapeseed oil, peach kernel oil, castor oil, cottonseed oil, peanut oil, sea turtle oil, oyster sauce, egg yolk Oil, palm oil, palm kernel oil, lacquer wax, coconut oil, butter, lard, squalene, squalane, decane or a hydride of such oils (hydrogenated oil, etc.).

(2) Wax

Beeswax, carnauba wax, cetyl wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, canary wax, montan wax, shellac wax, rice wax, and the like.

(3) Mineral oil

Liquid paraffin, petrolatum, paraffin, ozocerite, pure ceresin, microcrystalline wax, and the like.

(4) Fatty acids

Lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid, linoleic acid, docosahexaenoic acid, eicosapentaenoic acid, 12-hydroxystearyl Natural fatty acids such as acid, undecylenic acid, tall oil, lanolinic acid, isophthalic acid, caproic acid, 2-ethylbutyric acid, isovaleric acid, 2-methylpentanoic acid, 2-ethylhexanoic acid And other synthetic fatty acids.

(5) Alcohols

Alcohol, isopropanol, lauryl alcohol, cetyl alcohol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytol, phenoxyethanol and other natural alcohols, 2-hexyl decyl alcohol, isostearyl alcohol, 2 a synthetic alcohol such as octyldodecanol.

(6) Polyols

Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene Alcohol, propylene oxide, propylene glycol, polypropylene glycol, 1,3-butanediol, pentanediol, glycerin, pentaerythritol, threitol, arabitol, xylitol, ribitol, galactitol, sorbitol, Mannitol, lactitol, maltitol, and the like.

(7) Esters

Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleic acid ester, decyl oleate, octyl dodecyl myristate , hexyl decyl dimethyl octanoate, cetyl lactic acid, myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol Stearate, propylene glycol dioleate, and the like.

(8) Metal soap

Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate, and the like.

(9) Colloid, sugar or water-soluble polymer compound

Acacia gum, benzoin gum, damascus gum, guaiac gum, irish moss, karaya gum, tragacanth, locust bean gum, cypress seed, agar, casein, lactose, fructose, sucrose or its ester, trehalose Or a derivative thereof, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl group (C2 to C4) alkylene oxide added with ethylene oxide or the like (C2 to C4) chitin or chitosan, low molecular chitin or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or its salt, Hyaluronic acid or a salt thereof, chondroitin sulfate or a salt thereof, heparin, ethyl cellulose, methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, Hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinylpyrrolidone, polymethacrylate, polyacrylate, polyethylene oxide or Polyalkylene oxide such as polypropylene oxide or its crosslinked polymer, carboxyvinyl polymer, polyethyleneimine .

(10) Surfactant

Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactant (alkylamine salt, alkyl quaternary ammonium salt), amphoteric interface Active agent (carboxylic acid type amphoteric surfactant (amine type, betaine type), sulfate type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate type amphoteric surfactant), nonionic surfactant (ether type nonionic surfactant, ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing nonionic surfactant), and other interfacial activity (natural surfactant, derivative of protein hydrolysate, polymer surfactant, surfactant containing titanium-rhodium, fluorinated carbon-based surfactant).

(11) Various vitamins

Vitamin A group: retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (original vitamin A); vitamin B group: thiamine hydrochloride, sulfur Amine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acid, pantothenic acid, biotin, choline , inositol; vitamin C group: vitamin C acid or its derivatives; vitamin D group: ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydro tachysterol; vitamin E group: vitamin E Or its derivatives, ubiquinones; vitamin K group: leaf naphthoquinone (vitamin K1), methyl hydrazine (vitamin K2), menaquinone (vitamin K3), menadione (vitamin K4); , essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, whey acid, vitamin P (rutin, eriocitrin, hesperidin), vitamin U and the like.

(12) Various amino acids

Proline, leucine, isoleucine, sulphate, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, aspartame, glutamine , serine, cysteine, cystine, tyrosine, valine, hydroxyproline, aspartic acid, glutamic acid, hydroxy lysine, arginine, ornithine, group Amine acid or the like, or such a sulfate, phosphate, nitrate, citrate or pyrrolidine carboxylic acid-like amino acid derivative.

(13) Various additives derived from plant or animal materials

These processes are carried out according to the usual method according to the type and form of the product to be added (for example, pulverization, milling, washing, hydrolysis, fermentation, purification, pressing, extraction, separation, filtration, drying, powdering, granulation). It can be arbitrarily selected from various materials, such as dissolution, sterilization, pH adjustment, deodorization, decolorization, and the like.

In addition, as for the solvent to be used for extraction, it is generally preferred to use water, selected from the following organic solvents, in consideration of the purpose of use, the kind of the product to be provided, or the processing to be performed thereafter. a mixture of one or more of them: a lower alcohol such as methanol, ethanol, propanol, isopropanol, butanol or isobutanol or a lower alcohol, a polyol such as propylene glycol, 1,3-butanediol or glycerin or Aqueous polyols, various organic solvents such as acetone and ethyl acetate. In the case where it is preferred to use an organic solvent in view of the use, it may be used alone or in the form of any mixed liquid with water, using only water or ethanol which is easily removed after extraction. Extract extraction is available.

In addition, when the additive derived from the plant or animal raw material is supplied to a whole body or a topical external preparation or a cosmetic, it is expected to protect the skin or the hair, and the following effects can be expected: moisturizing and improving the feeling of touch, Texture, imparts softness, relieves irritation, relieves stress by aroma, activates cells (anti-cell aging), inhibits inflammation, improves skin texture, hair texture, prevents and improves skin roughness, hair growth, hair growth, anti-hair loss, and luster , cleansing effect, alleviating fatigue, promoting blood flow, warm bath effect and other cosmetic effects, in addition to expecting fragrance, deodorization, viscosity enhancement, anti-corrosion, buffering and other effects.

Further, in addition to the above, various cosmetic effects and pharmaceutical effects of the raw material materials known so far can be expected, and the target effects of the present invention can be enhanced by combining them or a product which is expected to have a multi-functional effect.

Specific examples of the specific plant (natural medicinal material) to be used as raw materials include almond (almond), indigo (blue leaf), Sabia japonica Maxim, Aucuba japonica, paulownia or sylvestris (Firmiana simplex), and wood defense (Cocculus orbiculatus), Acacia, Acacia, Astilbe thunbergii, Redcurrant "fruit", Red clover, Red grape, Ink tree (Sumu), Wild paulownia (Red cypress), Valerian Or Rubia cordifolia, Rehmannia glutinosa, assafoetida, autumnal pine, Ulmus parvifolia, Aguay Guacu, ko Mauritia flexuosa, Akebia quinata, Moraceae, purple petunia or morning glory (Planta), Hydrangea macrophylla, Angelica keiskei, Vigna angularis, Luo Hanbai, Acerola, Uncaria gambir, Canna edulis, Anise, Golden Fruit, Peach, Rapeseed, Cork, Fruit, Avocado, Flax, Sweet Tea (Hypothecium), Gynostemma , Yuzhu (P Olygonatum odoratum), Amana edulis, amaranth (Aphididae, Yanlaihong, Taisui, Qiansuigu, Red sorghum, Green sorghum, Green scorpion, Robinia, Green Persimmon, Amaranth) Fritillaria verticillata, alstroemeria, alkanet, algarrobo, hollyhock, arnica, argyi or ginseng (Alpinia katsumadai Hayata), aloe (aloe) ), Fira aloe, angelica, almond or Prunus armeniaca, Styrax benzoin, yellow persimmon, Elephantopus scaber, Epimedium grandiflorum, bluegrass , Fallopia japonica, Taxus cuspidata, strawberry, fig (carica "fruit, leaf"), iris (Iris tectorum), ramie (winter), Japanese sedge (Pyrola japonica), Ginkgo biloba ("seed, leaf"), Polygala tenuifolia, long-horned bean, shit (scorpion), Amaranthus viridis, rice "seed, seed coat", dill "seed" , Ibrahim thyme, nettle, perfume tree, borage (Conandron ramo Ndioides), Selaginella tamariscina, Kidney Bean, Oolong Tea, Fennel, Genipa americana, Bolboschoenus fluviatilis, Hawthorn vine, Fruit, Fennel, Yujin Curcuma longa), duckweed (Spirodela polyrhiza), asarum or dwarf scorpion or Asarum sieboldii, mallow, thin red sunflower, phalaenopsis, Prunella vulgaris, angelica or earth angelica (white peony, Udo, angelica), Uncaria tomentosa, black tea, plum (ebony, "seed, pulp"), white-backed peony, arbutin (uva-ursi), dried tangerine peel (Citrus unshiu), cloud Eleutherococcus senticosus, Echinacea, Echinacea, Finch, Flammulina velutipes, Senna obtusifolia, Gentiana, Bone Muguo "fruit", Luzon olive, apricot mushroom or Pleurotus eryngii, Sophora japonica, pea, Astragalus membranaceus, Polygonatum falcatum, Wang Wei Snow or 笹Snow, Coptis japonica, large sword, Trichosanthes bracteata, Inula helenium, Han Fang (anti-self), psyllium (Plantago asiatica), Zhangzhou Lulu (Pulsatilla, Rhaponticum uniflorumv), Corylus heterophylla, giant saguaro, Atractylodes. macrocephala, Lagerstroemia speciosa, Galla chinensis, fruit, Eriocaulon buergerianum, Da Shi Red iron, Da Shishan or Hawthorn (Hawthorn), Da Shi coconut, Hordeum vulgare, C. sylvestris, Cnidium monnieri, Okra "fruit", Inula britannica,禹州漏芦 (Echinops latifolius), Korean ginseng or bamboo ginseng (ginseng), young grass or millet leaf cutting grass or Western cultivar (Hypericum erectum), scorpion (Lamium album), Xanthium strumarium, Hickory, Hawthorn or Dioscoreaceae, Gastrodia elata, Lily or Lilium, Lilium lancifolium, Tail, and Acupuncture , Ulmus laciniata, Patrinia scabiosifolia, tuber 藜, Dutch mustard (cresson), parsley, psoralen, celery, olive "fruit, seed, leaf", oregano, orange "fruit , Peel, Huaiqing Dihuang (Rehmannia glutinosa), Cocoa "fruit, peel, seeds", persimmon (Persimmon "Leaf"), Uncaria rhynchophylla, Money Mint or Glechoma hederacea, Lily of the Valley , sage, lentils, Broussonetia papyrifera, "fruit", Curcuma zedoaria, eucalyptus (Quercus dentata), calyx, buckthorn, silk flower, willow, flower, sea Lygodium japonicum, calamus, carnation, Valeriana fauriei, birch, birch or birch (Betula pendula), gerbera, pumpkin, kapok, seeds, Zanthoxylum bungeanum, Typha latifolia ), Chamomile or Roman Chamomile, Tetrapanax papyriferus, Myrciaria dubia, leeches, Capsules, Trichosanthes cucumeroides, Pinellia ternata ), buckwheat, guarana "seed", Magnolia obvata, paulownia, Wolongmei, Chaenomeles sinensis, Fumu, Musk, Potentilla (Potentilla chinensis), water Bitter, Yunzhi, Dianthus superbus or buckwheat (Dianthus superbus), Artemisia carvifolia, Artemisia capillaris, Euphorbia kansui, Glycyrrhiza, Glycyrrhiza Tara agave, candelabra, hot river dandelion, canna, olive, raspberry (cabbage, raspberry, multi-gland raspberry, raspberry, maple berry, European raspberry), kiwi fruit , leaves, trichosanthes kirilowii, platycodon (Platycodon grandiflorus), chrysanthemum (Citrus, Chrysanthemum, Chrysanthemum), safflower, catalpa ovata, Rumex japonicus, phase ( Immature Orange), Ivy, Big Aloe, Summer Spearmint, Quinine, Cinchona Tree, Cinchona, Quino, Phellodendron amurense, Pigeon, Booty Vine, Ghost Face , cabbage, folder (Cabebu ) "Unripe fruit", Ge Wei, cucumber, Xihe Liu (Tamarix tenuissima), stone alkali wood, saponin tree, Ajuga decumbens, kumquat fruit, golden turtle tree, silver world lily , Jinwufan cactus, dipping sauce, Agrimonia pilosa, jinlan, silver orchid, golden edge orchid, Guapeba-Vermelha, guava "fruit", ebony rubber tree, no food Quercus infectoria, macadamia, head sylvestris, scorpion (枸杞, 枸杞, 枸杞, 枸杞), Asparagus cochinchinensis, georgum, eucalyptus, gooseberry, fruit, artemisia (Artemisia annua), Cutitiliver, Gardenia jasminoides, Quercusacutissima Carruthers, Cocoa flower, Mountain Cang, Mountain White Bamboo, Verbena officinalis, Sophora flavescens (Sophora flavescens), cranberry "fruit", chestnut "seed, fruit, endothelium", broad-leaved curculigo "fruit", grape "fruit, leaf", lobular buckthorn, iron holly (Ilex rotunda), lying dead Grass or hairy fragrant tea dish or extension Rabdosia japonica, clove (clove), saliva (Thlaspi arvense), schizonepeta (Schizonepeta tenuifolia), cockscomb (Celosia argentea), sassafras, laurel (Laurus) Nobilis), Smilax glabra, Gentian, Geranium thunbergii, Hovenia dulcis, Western dandelion, Kishu mandarin (citrus, tangerine, red mandarin orange, buckwheat mandarin, phase model) Citrus, ponkan, orange (orange), rose chinensis, perfume mint, 楮 "fruit", black tea, Nuphar japonicum, 槁本(唐槁本, Ligusticum sinense) , sorghum, galangal (Alpinia officinarum Hance), 芫荽 "fruit", Picrorhiza kurrooa, Scutellaria baicalensis, moss (Bilberry), coconut "fruit", Tetradium ruticarpum, raspberry ( Rubus chingii), Piper nigrum, Cosmos, Cuban fat, coffee "seed, leaf", Magnolia kobus, burdock (Arctium lappa), small peony, sesame (Orienta l sesame), Scrophulariaceae, Schisandra, Schisandra or bone snake or pine vine, wheat, rice or rice bran "red peony, white peony", rice oil, Sudan cola fruit "seed", Cora Bella "Seed", Thorn Lishan, Fenugreek "fruit", African anti-self, South American milk vine, kelp, medlar, Symphytum officinale, sisal (Agave sisalana), azalea, nesting orchid (Neottia nidus -avis), Clematis or Hundred Roots or Clematis chinensis Osbeck, Sakura (Oshima Sakura, Sakura Sakura, Oyama Sakura, Edo Sakura, Bean Sakura, Sakura Sakura, Tokyo Sakura, Kaolin Sakura , Xia Ying, Xiao Bian Ying, Li Ying, Han Ying, "Leaf, Flower, Fruit, Bark (Sakura)"), cherry, pomegranate, medlar, eucalyptus, orchid, Camellia, Alisma plantago-aquatica, Satsuma, Sugar cane, sugar beet, Ziziphus jujuba, saffron (Crocus sativus), ginseng fruit, yolk fruit, Zhu Xi "fruit", saponin, berry "fruit", Cimicifuga simplex, sand blue thorn Head (Echinops gmelinii), sage (ocean Su), Lobelia sessilifolia, Pterocarya rhoifolia, celestial scale, Croton Lechleri, three-leaf clover, Pseudoginseng, Cornus officinalis, Zanthoxylum Piperitum), Euchresta japonica, shea, shea, fruit, Lentinula edodes, aster (Aster), four-corner, digitalis, Quisqualis indica, Perilla or cranberry or succulent perilla or one-sided perilla (perilla, perilla), red sandalwood, eucalyptus, eucalyptus, forsythia suspensa, mushroom (hinshimeji, brown mushroom, antler mushroom, white mushroom, crab mushroom, flat Mushroom, Oyster Mushroom, Mulan Magnolia, Lower Wild Grass, Potato, Adenophora stricta, Jasmine, Ophiopogon japonicus, Pentagon Carob, Perennial Radix, Amomum vulgaris Ren, Amomi Semen), 薏苡, palm "fruit", Chunlan, Queen's sunflower, Zingiber officinale, Dichroa febrifuga, Acorus calamus, Acorus calamus, Shozuku "fruit", white peony "seed", white and (Bletilla striata), white hummingus "seed", white stalk (clover), scorpion mother (Abrus precatorius), iris (Iris florentina), white flower vine "flower", yellow Lu Binghua, Shironanten, Cinchona succirubra, Daxiang (Daphne odora), Cinnamon, Cinnamomum cassia, Watermelon, Honeysuckle (Lonicera japonica), Rumex acetosa, Swimberry Fruit, Venus Fruit, Stevia, Strawberry Fruit, Vincetoxicum pycnostelma, Equisetum arvense, Purslane (Portulaca oleracea) ), plum "fruit", Cymbidium ensifolium, European red pine "fruit", western larch, western ivy, western walnut, Ou Shan, Western dandelion, horse chestnut (horse chestnut), western pear Fruit, Western Summer Grass, Western Elder, Western Juniper (Juniperus), Western Sawgrass (millefolium), Western Rose, Western Wind Butterfly, European Mistletoe, Western Lotus or Western Mountain Mint, Western Wasabi, Acorus tatarinowii, Dendrobium (Sakura 斛, 金钗石斛, 大花石斛, Okinawa Dendrobium, Dendrobium, Dendrobium, Spider Stone, Yellowstone) (Dendrobium, Dendrobium ), deodorant wood (Lippia citriodora), Qiankui, Meiyuanzhi, Yangyuanzhi, celery, celery, Ligusticum chuanxiong, AndroGraphis Paniculata, bitter, thorny cactus, mountain lentils, fruit, leaves, iron Clematis terniflora, swertia, millennium wood, Amomum tsao-ko, white peach fan, vegetarian heart, buckwheat, seed, yam, broad bean, rheumat, radish , soybean, orange (orange peel, medlar), southeast blue thorn (Echinops grijisii), Eclipta thermalis, Euphorbia lasiocaula, Cibotium barometz, black sweet cherry "fruit" , Litchi, Thyme (Thymus vulgaris), Dioscorea gracillima, Liliu, Tamarind, Seed, Onion, Magnolia salicifolia, Elm "fruit, leaf, root bark", (large leaf Holly (one leaf tea, Kuding tea, bitter tea, Ilex latifolia), dahlia (Scorpio peony), variegated lupin (Taurvi), deer scorpion, single thorn cactus, salvia miltiorrhiza, tarragon (Taraxacum) Or white dandelion or Mongolian dandelion, tamarind, cherry "fruit", white stalk "fruit, root, bud", Panax japonicus, chicory, Pyllanthus niruri, tulip, chocolate nourish (choko-no-stei), artichoke, rhubarb (rhubarb), scorpion (Dalian), mugwort (Polyporus umbellatus), grass worm, evening primrose, Neottia Kiusiana), mushroom, blood red meat, camellia, centella asiatica, Sagina japonica, Commelina communis, kidney bean (red adzuki bean), inverted spider plant, F. sylvestris (Fallopia multiflora) , Tetragonia tetragonioides, Codonopsis lanceolata, wild soybean, mountain chrysanthemum, sea red bean, walnut, dewberry "fruit", Amakusa, sweet tea (Leaf of Strigose Hydrangea), Tiantai Wu medicine (Uygur medicine), Winter melon (winter melon), hook vine Capsicum annuum, Angelica sinensis, marigold, tang pod, Dipsacus asperoides, corn or corn silk, Ligustrum lucidum, gentian or Korean dragon Gentiana uchiyamai, Equisetum hyemale, Houttuynia cordata, Carapichea ipecacuanha, Cuscuta or Southern Cuscuta or Cuscuta, Eucommia ulmoides ("bark, leaf, root"), Japan F(Fraxinus japonica), tomato, gum scutellaria, acacia seed, eustoma, valerian, sweet poplar, geranium, fragrant bean, Nigerian berry "fruit", yam or mountain potato (Dioscorea japonica), false leaf tree (butcher's broom), camphor, leeks, rapeseed, bean or red peas (Canavalia gladiata), summer mandarin orange, jujube (Ziziphus jujuba), Rosa laevigata, Japanese dipsacus japonicus, slider Mushroom, peanut, Nandina domestica, ramie (Boehmeria nivea var. nivea), camphor, Picrasma quassioides, Artemisia absinthium, nutmeg, osmanthus Or cinnamon or ceylon cinnamon or cinnamon (sweet cinnamon) or cassia (salmon), female peaks, allium tuberosum, sambucus (Sambucus racemosa, "fruit, flower, stem, leaf"), garlic ( Allium sativum), Rhus javanica, Onion, Albizia or Acacia or Albizia or Albizia julibrissin, Wild flor, Rosa multiflora ), phoenix cockscomb, saw grass, anthus (Angelica decursiva), wild rose, mountain peach, pineapple "fruit", hibiscus (hibiscus flower, wind bell hibiscus, Luoshen), gentian, lupin, wood tree (pau) -doce), Drynaria roosii, Mucuna Birdwoodiana Tutcher, White Mountain Thousand Birds, White Stork (White Stork), Bilberry Fruit, Stellaria media Stellaria, Corylus heterophylla ), 莨若(莨若根), basil, lotus (lotus, lotus, lotus seed), parsley (parsley), rye, sweet potato, light bamboo or bamboo (bamboo), fragrant fragrant, mint (Mint leaves, Mint ), Coix lacryma-jobi, Anemarrhen a asphodeloides), rubber, flower mint, scorpion (scorpion scorpion, schisandra), vanilla bean, papaya, Gnaphalium affine, Paharobobo, Bobgrass Stems, leaves, green peppers, succulent orchids, vines or Vitex rotundifolia, sedge (scented aconite), scorpion (Tribulus terrestris), simmered eggplant (rose), coral (river wind), Witch hazel, palm tree, rose, balata, balata rubber tree, mushroom (mushroom, wild mushroom, ball-based mushroom), ylang, green money (western jujube), summer white chrysanthemum, Orchid, Impatiens (vine lychee, bitter gourd), spring 榆 (榆, 榆白, 榆), 多花蓣, Chunlong, Semen Sterculiae Lychnophorae, bread tree, Iris domestica, Codonopsis Codonopsis pilosula), Pecans, Bishui (Limece, Manchus), Brassica peony, Rough Blue Thorn, Ling (Pellet), Pistachio, Beetroot, Pyrrosia lingua, Dyestuff , daisy (Bellis perennis), Achyranthes bidentata, Orchis chidori, eucalyptus, 桧, ramie, sunflower, sweet pepper, Semiaquilegia adoxoides, Typha domingensis, Agaricus blazei (Brazil mushroom, Brazilian Agaricus blazei), sharp-lipped bird nest orchid, sweet pepper "fruit", white peony, white and glutinous rice Branch "fruit", raw beans, lentils, mallow, Pulsatilla chinensis, 枇杷 "fruit, leaves, stems", betel nut (big belly skin, betel nut), Pu'er tea, wind vine (Nanteng), 蕗, coltsfoot (cold winter flower, coltsfoot leaf), Zeeland (bluegrass), vine bean (lentil), scented flower (small cherry, scented flower), grape "fruit, peel, seed, leaf", beech, Cordyceps sinensis , Brazil licorice, Brazilian ginseng, Brazilian coconut, Brazilian palm, blackcurrant "fruit", blackberry, Blarin, Japanese peach "fruit", stalk algae, blueberry (European), prune, florola Floral Blanca, psyllium, mung bean (Phaseolus aureus), medlar, loofah, winter phoenix, safflower (Carthamus tinctorius), kenaf, belladonna, berry "fruit", avocado, beru Peruvianburg, Eucalyptus unicorn, Sedum or Crassulaceae, Boisenberry "fruit", Kochia or Tsingtao or Hajime (Bassia scoparia), Pregnancy orchid, Impatiens (Fengxian, acute, translucent), Meiguolan (Pouteria sapota), yolk fruit, pudding, Saposhnikovia divaricata, spinach, palace lantern (sour syrup root), Park Shu (and Magnolia, Park), papaya (Chaenomeles speciosa), rock fragrant (bitter chrysanthemum), Atractylodes lancea, Aizoon stonecrop, echinacea, Tilia miqueliana, peony (Paeonia suffruticosa, "flower, leaf, stem, bark"), cortex moutan ), bluff, jojoba, borage (Borago officinalis), daisy, moss "fruit", Cistanche (Cistanche salsa), Dendrobium (Dendrobium officinale), Maizuru Tenan (Tiannanxing) , Grifola frondosa, Ephedra sinica, maca, macadamia, daisy (Marguerite), seaweed (Digenea simplex), mulberry (mulberry white bark, leaf), Wudang Magnolia, Guyana Iron wire, New Zealand Christmas tree, iron Tree, nasturtium (nasmosa, bulbous nasturtium), Actinidia polygama, pine ball, pine "leaf, bark, root", P. salicifolia, wolfiporia extensa, narrow leaf pepper Tree, oregano leaf (flower mint), Sophora flavescens, mulberry "fruit", hazelnut, Verbascum thapsus, mango, mangosteen, chamomile (Manzanilla), walnut, Chinese orange "fruit", Ganoderma lucidum (Ganoderma lucidum), Immature Orange (fruit), Mishima Bupleurum (Bupleurum), Agaricus or Ottelia alismoides, Ottelia alismoides, Lythrum anceps, Polygonum aviculare, sleeping vegetables, clover, green fruit, green mint, mimosa, lotus, miracle fruit, myrrh, cherry plum, jasmine, hibiscus (Hibiscus syriacu), Rough tree, sapindus (dead skin), Munya, Lithospermum erythrorhizon, purple or narrow-leaved purple (Callicarpa japonica), purple corn, purple summer vine (Kunming spatholobus), single-sting cactus, Motherwort (Leonurus sibi) Ricus), nine-story tower, white layer, fragrant bee flower, grass hibiscus, melon "fruit", Mongolian artemisia, Mengzong bamboo, wood unicorn, pepper (Schinus molle), whole holly, woody (Saussurea costus), palm leaf Yellow, peach (peach, "leaf, seed, flower, fruit"), Zhuman bud, Morillo black cherry "fruit", jute (Corchorus olitorius), Bachuan purple sable, puzzle (Alpinia oxyphylla), cornflower (ghost lamp檠), Cornflower Mint, Yagong, Yaksha Gallic (Yaika) or Yekura Fork Gallnut or Euonymus fortunei Gallium "fruit, peel, fruit 穂", 谷地柳, 八角金盘 (Fatsia japonica), parasitic wood (willow parasitic), Liu (Chuanliu , Liliu, weeping willow, red bud, silver willow, golden silk, willow, willow, willow, willow, deep willow, coconut, mountain willow, big leaf willow, pseudo-salt, fox willow, sweet poplar) Willow leaf "leaf, stem", blackberry, Ardisia japonica, Tianshenqing (Carpesium abrotanoides), mountain burdock (Shanglu), old alder (Hawthorn), Yangmei (Yangmei) Pi), Mountain Artemisia, Eucalyptus, Saxifraga stolonifera, Yucca or Lin Lan, grapefruit "fruit", lily, Chuanbaiyu, May Ai (Ai Ye), Polyporus (Ray bacterium), Lime "fruit", rye, Luo Han Guo "fruit", raspberry "leaf, fruit", thorn Capsule (Krameriaceae), alfalfa or shallot (薤白), lavender, longan (longan meat), agave (green agave, agave), green tea, apple, fruit, seed, leaf, root 』, gentian, raspberry or sweet tea (sweet), glaze (Xinjiang blue thorn, Echinops ritro), lychee (lychee, litchi nucleus), lettuce (Lactuca sativa), red currant "fruit", red heart Dragon fruit, lemon "fruit", lemongrass, forsythia or Chinese forsythia (Forsythia), lotus flower, wax plum (wax), palm, reed (reed, Phragmites communis), roganberry "fruit", rosemary ( Wannianxiang), rose hips (rose), mustard, Buddleja Officinalis, and mywood (mantle).

As the seaweed, the following seaweeds are exemplified: seaweed [green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidea, moss (edge tube) Brassica, green sphagnum moss, sphagnum moss, sphagnum moss, sphagnum moss, sarcophagus (salt), seaweed [brown algae: kelp (true kelp, lycopene, fine kelp, three stone kelp), Wakame, forked algae, green wakame, giant seaweed (Macrocystis pyrifera, Macrocystis integrifolia, Nereocystis luetkeana), deer, Fucus, Phyllostachys , southern squamata, cynomolgus, sphaerophyte, sphaerocephala, Padina crassa Yamada, Padina japonica Yamada, sphaerophyte, Padina stipitata Tanaka, seaweed [red algae] : Trichophyton, Gelidium amansii, Knife-shaped Cauliflower, Japanese broccoli, J. chinensis, Pheasant, Cucumber, Chicken, Lychee, Celosia, Eucheuma, Philippines Eucheuma, Lincai, cypress, eucalyptus, carrageen, big leaf carrageen, Chondrus crispus, wedge carrageen, thorny carrageen, pinch carrageen, long carrageen, heterochromatic Carrageenan, Japanese carrageen, Pythium, Phaeocystis elegans, Phaeocystis chinensis, Phaeocystis chinensis, Cyanophyta, Dendrobium, Pseudomonas, D. Algae, etc.

In addition, other algae, such as green algae (Chlamydomonas: Chlamydomonas, Iced algae; Dunaliella: Dunaliella; Chlorella: Chlorella; Shigella: Shigella; Group: Spinning shell Euglena, group algae; genus genus; genus genus; genus: spirulina, sphagnum, genus, genus, genus: genus: genus: sarcophagus莼石莼,长石莼; Japanese brook: Japanese brook vegetables; Phytophthora; Cladosporium: Japanese clostridium, light green genus, genus Cladosporium, chlorella; cyanobacteria: large bubble method Cyanobacteria, cyanobacteria; bananas: bananas; Brassica: Brassica, Brachypodium, Schistosoma, Caulerpa scalpelliformis; Feathers; : pine algae, sub-cylindrical pine algae, thorn pine algae, long pine algae, flat pine algae; cyanobacteria: cyanobacteria; genus Chaetoceros: tricons, beetle, algae, spirulina; new Genus genus; Chaetoceros; Cyanophyta; Tennis genus: Tennis algae; Reef genus: reef membrane, wide reef membrane, format reef membrane; Cohen algae; Helicobacter; Panicula; Genus: 堇Algae; binary sp; non-compartmental sp, etc.).

Cyanobacteria (Cryptococcus: Cryptophyta; Microcystis; Candida: Gexianmi, Dimumu, Hairy; Quercus, Spirulina: Spirulina, Trichodesmium ) is a genus).

Brown algae (Spirulina: spirulina; brown genus: Indian brown geranium; porous alga: porous alga; brown genus: brown algae; sphaerotheca: trichophyton; genus:蜈蚣藻; 鞭 genus: chlorella, dipterophyte, chlorella algae; genus algae: net algae, fork open net algae; thick cyanobacteria: thick net algae, thick margin algae; Algae: Diptera: Diptera, Diptera, Membrane, Diptera; Brown leaf algae; brown leaf alga; seaweed: seaweed, small group of fan algae, Poli fan Algae; Brachypodium genus: Elachista taeniaeformis; Chaetoceros: Alternaria: Mucosa: Mucosa; Rhizoctonia: Rhizoctonia solani; Cyanophyta: Pine alga; :Suo algae; Okamura genus: Okamura genus; genus: silk algae; noodle genus: noodle alga; spirulina: spirulina; Hydrilla: Hydrilla verticillata; Algae; Haiyun genus: Haiyun; Iron genus: Iron-boiled vegetables, Iron-nailed vegetables; Brown-selling algae: brown pine algae; Trichophyton: Trichophyton; Black-headed genus: Black-headed algae; Acid-algal: acid Algae Desmarestia virdis, Desmarestia tabacoides; Thick-spotted genus: Thick-spotted algae; Anopheles: Anopheles; Chickweed: Chickweed; Agrocybe: Leaf Algae; Phytophthora: Coccinella; Coilodesme genus: Coilodesme; Cyanobacteria: Cyanobacteria, M. sylvestris; Pyropia kurogii genus: Cyanobacteria (Pyropia kurogii); sea net algae: sea net algae; group of thousands of birds: group of thousands of birds; species of the genus Stictyosiphon: Stictyosiphon; rock genus: rock whiskers; Genus: Cyclosporium; Algae: Algae; Net-tube algae: Net-tube algae; genus genus: spirulina; genus genus: Phyllostachys pubescens; Polycystis: Polycystis; Cymathaere Genus: Cymathaere, Cymathaere japonica; Laminaria: Japanese kelp, true kelp, kelp (Saccharina japonica), sugar kelp, long kelp, Saccharina yendoana, Saccharina cichorioides ); genus Alzheimia: diarrhea; genus Anaba: amphibians; cavity kelp: cavity kelp Amaranth cloth, black cabbage; Asteraceae kelp: Asteraceae kelp; 捻Kunbu genus: 捻Kunbu; Streptophyllopsis genus: Streptophyllopsis; Phytophthora: Phyllostachys pubescens; : Phyllostachys pubescens; genus genus: genus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus, genus Stephanocystis hakodatensis ), Mycorrhopsis yendoi Fensholt; Sargassum: Japanese sargasso, S. cerevisiae, Sargassum segii Yoshida, Red horsetail algae, Sargasso, Sargasso, algae , Sargassum nasa, Sargassum piluliferum, Sargassum sylvestris, Astragalus sphaeroides, S. cerevisiae, Sargassum, Sargassum, Artemisia, Algae, Spinosa, Sargasso, Spirulina, Big leaf Spirulina: Coccophora: Coccophora; Macroalga: Macroalga; Cyanocystis: Cyanocystis, etc.).

Red algae (Red genus: red cabbage, small red cabbage; laver: Asparagus latifolia, stripe laver, long leaf rock laver, long laver, Wildemania, multi-color laver (Wildemania variegata), Wide seaweed (Wildemania amplissima); red genus: Bangia gloiopeltidicola; genus: genus; genus: genus, genus, Otohimella japonica, soft powder Phyllostachys pubescens; sea sapphire: sea sulphate, spirulina, cuspidatum; genus genus: genus Verticillium, genus Quercus; stone pine: stone pine; seaweed: seaweed; Pseudomonas seaweed: pseudo-room seaweed; dinoflagellate: dinoflagellate; genus Alternaria: genus Alternaria, larvae; Ptilonia okadae Yamada genus: 畑 畑 田 ;; Genus: Phyllostachys pubescens; Cypress genus: Phyllostachys pubescens, Cypress cyanobacteria; Stone broccoli: small stone cauliflower, hidden fruit cauliflower, big stone cauliflower, southern grass, Gelidium tenue, hydrangea, whiskers Phyllostachys pubescens; Phyllostachys pubescens: Dunaliella, P. sylvestris; Lepidoptera: Bright tube alga; Red algae: Red algae; Adhesive Algae: Algae, Algae, Algae; Masudaphycus: Masudaphycus; Seaweed: Large-leaved sea bream; Neodilsea: New algae, Rhododendron (Neodilsea tenuipes Yamada); genus Pinnacle: Phyllostachys pubescens, cedar algae; Phytophthora: Phyllostachys pubescens; Cladophora: Cladophora; Auricularia: C. occidentalis; Cruoriopsis) genus: B. chuanxiong; P. algae: P. algae; coral genus: coralline alga; genus genus: algae, Phyllostachys pubescens, Phyllostachys pubescens, Phyllostachys pubescens , tilapia, succulent algae, elliptic algae, scorpion algae, lanceolate, algae; sea genus: algae, black worm, algae; Gold genus: Pingjinshi; Haibai: Haibai; Sawtooth: Hard Shield, Alga, Spirulina, Carpopeltis flabellata, Grateloupia crispata, Fork Shielded fruit algae, Prionitis articulata, Sawgrass, and Prionitis elata Spirulina serrata; genus genus: large leaf gold; genus: genus; genus Schimmelmannia: long tail feather; genus: Gloiopeltis, Gloiopeltis furcata, antler Dirocarpus; Tichocarpus genus: Dioscorea; Dioscorea: Trichoderma lucidum, Callophyllis crispata, Palmetto phylum, Adhesive pheasant, Adhesive cockscomb Calla algae, Callophyllis cristata, Callophyllis hayamensis, Calliflora genus, Callophyllis rhynchocarpa Ruprecht, Pugetia genus: Pugetia; (Cirrulicarpus) genus: Trichophyton; genus: Kallymenia sessilis, Kallymenia sagamiana, Kallymenia callophylloides, Schmitzia genus: Japanese history M. genus; Zengzao: Zengzao, Tsengia lanciforia; Phytophthora: smooth alga; genus: schizophrenia; seaweed (Halarachnion) genus: seaweed; Grass: Yamada grass; red dragonfly Genus: red leeks, fine leaf red leeks; cockroach genus: taro cockroach; small cockroach genus: small cockroach cockroach; genus genus: Phyllostachys; sea head red genus: sea head red, fine sea head red; Sandy genus: sandy vegetable, sassafras, spirulina (Hypnea variabilis), frozen sand, arbuscular genus: arbuscular algae; Stem genus: Stem algae, Auricularia: sea fungus; dragon Physalis: Asparagus, Asparagus, Chrysanthemum, C. sylvestris, R. sylvestris, R. sylvestris, D. sylvestris, Gracilaria rhodocaudata, Aurora sylvestris, Violet Sauerkraut, Gracilaria sublittoralis, A. sylvestris, Edible Ginger, Kirin Longcai, Gracilaria lemaneiformis, Zebra sinensis, Bowgrass, and radicans Vegetable, Umbrella, Brassica oleracea, C. sylvestris; Pseudomonas genus: Prunus persicae; Quercus genus: Qujiang dynasty; Tylotus genus: Phyllostachys pubescens; Acetidae; Pseudomonas: Iridium; Ignella: Ahnfeltia plicata, Ignella, Ahnfeltia paradoxa; Stenogramm a) genus: brookside fern; genus genus: algae, genus Gigartina ochotensis, cedar seaweed; wedge-shaped carrageenan: wedge-shaped carrageen; red genus: red genus; Gloioderma) genus: Phytophthora; cyanobacteria: Fauchea leptophylla, Fauchea stipitata; genus genus: chlorella, whole cryptococci; luminal gland Algae: Phytophthora, Aggregating genus, Chlorella: Grape algae; Cryptophyta: Cryptophyta; Palmatum: palm-shaped algae, palm-shaped genus (Palmaria palmata), with milk red Rhodmenia pertusa; Phytophthora: Phytophthora; Rhodophyta: Rhodophyta; Rhizoctonia: Cyclosporium, Algae, Lomentaria okamurae; Ring-connected algae: algae-connected algae, thin-leaf ring-connected algae; Xiancai: Xiancai, soft-scented celery, cone-shaped celery, Japanese celery, Bodeng celery; condensed genus: coriander, hooked cod; Small genus: Phyllostachys pubescens; Blue genus: Blue-green algae; Phyllostachys pubescens: Feather double seaweed, seaweed, Neoholmesia japonica, Top genus: Top Algae; pseudo-multiple genus: pseudo-multiple venous; Neohypophyllum: Neospermia; Myriogramme: Hideophyllum yezoense; Top algae: algae with algae, algae of fan-shaped algae, algae of algae, algae; algae of Hymenena: Hymenena tenuis; red nets: red net algae绢光红藻属: 绢光红藻; Radiation algae: Sphaerotheca fuliginea; Heterophyllum genus: Japanese genus Algae, beautiful Heterophyta; Rhodopterum genus: Rhodoptera (Rhodoptilum plumosum); Rhodophyceus: Polygonum elegans, Polygonum occidentalis; Amaranthus: Amaranth; Cymbidium: Dendritic algae, Caragana angustifolia, Willow algae, Entangled cartilage (Chondria Intertexta), echinococcal algae, expanded cartilage algae, lanceolate cartilage, deciduous algae: scutellaria, scutellaria, scutellaria, red-spotted algae; genus: grass, Pterosiphonia fibrillosa; genus Spirulina: Phyllostachys pubescens, Daucus, D. elegans; : algae, black algae; algae: Amansia rhodantha, Amansia japonica, algae; initial algae: first algae; Aneurianna) genus: Leymus algae; sea bastonia: sea buckwheat; rattan algae: vine pine algae; tooth algae: Odonthalia corymbifera; genus genus: safflower, oblate, black bead Algae (Batrachospermum gallaei); hot spring red algae: hot spring red algae; hypothetical haema noodles (Nemalionopsis) genus: quasi-haisuo surface; polychaeta; algae: blood algae; blood strip moss: blood strip moss ).

Axillary algae (A. genus; genus genus; genus genus: star spirulina; genus genus, black genus: large genus, Nitella acuminata; bird's nest genus, etc.) Yellow algae (Golden algae: golden algae, etc.).

Also, as a material derived from animal materials: cockscomb extract, bovine or pig, human placenta extract, bovine or swine stomach, duodenum, intestine, spleen extract or its decomposition product, bovine or pig brain tissue Extract, collagen hydrolysate (acid decomposition product, alkali decomposition product, enzyme decomposition product, etc.) of cattle, pig or fish, or collagen derivative such as water-soluble collagen or deuterated collagen, elastin of bovine or pig Or an elastin hydrolysate (acid, base, enzyme, etc.) or a water-soluble elastin derivative, keratin and its decomposition products or derivatives thereof, silk proteins and their decomposition products or derivatives thereof, pigs or cattle Blood globulin breakdown (hemoglobin), bovine or porcine hemoglobin breakdown (hemin, hydroxyhemoglobin, heme, protohemoglobin, heme iron, etc.), milk, casein and its decomposition products or Such derivatives, skimmed milk powder and its decomposition products or derivatives thereof, lactoferrin or its decomposition products, egg components, fish decomposition products, nucleic acid related substances (ribonucleic acid, deoxyribonucleic acid) and the like.

Further, as a material derived from a plant or an animal material, any part derived from a genetic recombinant or a cell fusion, a cell, a tissue, an organ, a metabolite, or the like can be used. Further, for example, an undifferentiated cell population obtained by cell culture of any part, cell, tissue, organ or the like, or a cell population in the middle of differentiation may be used.

(14) Marine composition

Seawater such as deep water, such as sea salt, dried seawater, organic salt obtained from the Dead Sea or the Atlantic Ocean or the Pacific Ocean (sodium chloride, magnesium chloride, potassium chloride, etc.); sea mud or mud (mineral mud), for example Italian mineral mud, German mineral mud, love non-mineral mud, Freiburg mineral mud and other places of sea mud or mud (including ingredients: cerium oxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, potassium oxide, Magnesium oxide, calcium oxide, barium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese, arsenic, water), sacred stone, and the like.

(15) Microbial culture metabolites

Yeast metabolites, yeast extracts, bacterial metabolites, bacterial extracts, mold or actinomycetes metabolites, mold or actinomycete extracts, natto metabolites, natto extracts, rice fermentation extracts, rice bran Fermentation extract, red worm extract or its decomposition product or such water-soluble derivative, trehalose or its derivative, lactic acid fermentation product of raw milk or skim milk powder, lactic acid bacteria fermentation product of legume , lactic acid bacteria fermentation of coconut plants, and the like.

(16) α-hydroxy acids

Glycolic acid, citric acid, malic acid, tartaric acid, lactic acid, and the like.

(17) Inorganic pigments

Anthraquinone anhydride, magnesium citrate, talc, kaolin, bentonite, mica, mica titanium, bismuth oxychloride, zirconia, magnesia, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, iron oxide yellow, iron oxide, iron oxide Black, ultramarine, chromium oxide, chromium hydroxide, carbon black, calamine, and the like.

(18) UV absorption / blocker

Benzophenone derivative (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, 2-hydroxy-4-methoxydiyl) Sodium benzophenone-5-sulfonate, dihydroxydimethoxybenzophenone, sodium dihydroxydimethoxybenzophenone-sulfonate, 2,4-dihydroxybenzophenone, tetrahydroxydi Benzophenone, etc., p-aminobenzoic acid derivatives (p-aminobenzoic acid, ethyl p-aminobenzoate, p-aminobenzoic acid, p-dimethylammonium benzoate, p-dimethylene) Methyl octyl benzoate, etc., methoxycinnamic acid derivative (ethyl p-methoxycinnamate, isopropyl p-methoxycinnamate, octyl p-methoxycinnamate, p-methoxy cinnamyl) Acid 2-ethoxyethyl ester, sodium p-methoxycinnamate, potassium p-methoxycinnamate, di-p-methoxycinnamic acid mono-2-ethylhexanoic acid glyceride, etc.), salicylic acid derivative (octyl salicylate, phenyl salicylate, trimethylcyclohexyl salicylate, dipropylene glycol salicylate, ethylene glycol salicylate, myristyl salicylate, methyl salicylate, etc.), An ortho-aminobenzoic acid derivative (methyl ortho-aminobenzoate, etc.), a uric acid derivative ( Urinary acid, urinary acid ethyl ester, etc.), coumarin derivative, amino acid compound, benzotriazole derivative, tetrazole derivative, imidazoline derivative, pyrimidine derivative, dioxane derivative , camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives, umbelliferone, horse chestnut glycogen, benzyl cinnamate Ester, chinox, oxybenzone, dihydroxybenzophenone, octyl ketone, sulphonone, benzoquinone resorcinol, arbutin, guaiac, shikonin, begalin, shellfish Cain, berberine, Neo Heliopan, Eccyl Ethyl Cyanate, Zinc Oxide, Talc, Kaolin, and the like.

(19) Whitening agent

p-Aminobenzoic acid derivative, salicylic acid derivative, ortho-aminobenzoic acid derivative, coumarin derivative, amino acid compound, benzotriazole derivative, tetrazole derivative, imidazoline derivative , pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin C or derivatives thereof (vitamin C Magnesium phosphate, vitamin C glucoside, vitamin E or its derivatives, kojic acid or its derivatives, oxybenzone, benzophenone, arbutin, guaiac, shikonin, bega Ling, begain, berberine, placental extract, turmeric tannic acid, 4-n-butyl resorcinol, and the like.

(20) Tyrosinase activity inhibitor

Vitamin C or a derivative thereof (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), hydroquinone or a derivative thereof (hydroquinone benzyl ether, etc.), kojic acid or its organism, vitamin E or its Biological, N-acetyl tyrosine or its organism, glutathione, hydrogen peroxide, zinc peroxide, placental extract, kojic acid, arbutin, 4-n-butyl resorcinol, silk extract Plant extracts (chamomile, mulberry, medlar, angelica, mantle, Sophora flavescens, Ai, honeysuckle, Phellodendron, Houttuynia cordata, alfalfa, alfalfa, scorpion grass, fluffy cloth, hawthorn, eucalyptus, yarrow, Hollyhock, cinnamon, vine, North American witch hazel, mulberry, marigold, platycodon, dodder, continuation, shoot dry, ephedra, Chuanxiong, Duhuo, Bupleurum, wind, beach wind, scutellaria, peony, old Valerian, puerarin, licorice, gallnut, aloe, cohosh, safflower, green tea, black tea, Axian medicine, etc.).

(21) Melanin reduction/decomposition substances

Phenylmercury hexachlorophenol, mercuric chloride, mercurous chloride, hydrogen peroxide water, zinc peroxide, hydroquinone or its biological (hydroquinone benzyl ether).

(22) Metabolic promotion / cell activating substance

Hydroquinone, lactic acid bacteria extract, placenta extract, Antrodia camphorata extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (aloe , Astragalus, Equisetum, Gentiana, Burdock, Purple Root, Ginseng, Witch Hazel, Fudge, Coix Seed, Gardenia, Swertia, Angelica, Calendula, Sweet Tea, Young Cut Grass, Cucumber, Thyme, Rose, Parsley) Wait.

(23) astringent

Succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcinol, ferric chloride, citric acid (including catechin compounds), etc. .

(24) Active oxygen scavenger

SOD (Superoxide Dismutase), catalase, glutathione peroxidase, and the like.

(25) Antioxidants

Vitamin C or its salt, stearate, vitamin E or its organism, nordihydroguaiaretic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, p-hydroxyl Anisole, propyl gallate, sesame phenol, sesamelin, gossypol, propolis, etc.

(26) Lipid peroxide production inhibitor

--carotene, plant extracts (sesame culture cells, sweet tea, young cut grass, witch hazel, clove, fragrant bee, callus, white birch, sage, rose, ginkgo, tianzhu, camp, ginkgo, green tea) Wait.

(27) Anti-inflammatory agents

Fish stone fat, mersin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, dibenzoin hydrochloride, d-camphor, dl-camphor, hydrocortisone, Chuangpi, Jerusalem artichoke, chlorpheniramine maleate, glycyrrhizic acid or its salt, glycyrrhetinic acid or its salt, licorice extract, purple root extract, commercial extract, propolis and the like.

(28) Antibacterial, bactericidal, and disinfecting drugs

Aquinol, sulfur, calcium gluconate, chlorhexidine diglucoside, aminosulfonic acid, red bromomercury, lactoferrin or its hydrolyzate, alkyldiamine ethylglycine, Triclosan, sodium hypochlorite, chloramine T, bleaching powder, iodine compound, iodoform, sorbic acid or its salt, propionic acid or its salt, salicylic acid, dehydroacetic acid, parabens, undecylenic acid Thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p-chlorophenol, p-chloro-xylenol, p-chloro-m-cresol, thymol, phenylethyl alcohol, O-phenyl Phenol, Yujiexin CH3565, halkaban, hexachlorophenol, chlorhexidine, ethanol, methanol, isopropanol, benzyl alcohol, ethylene glycol, propylene glycol, 2-phenoxyethanol, 1,2- Pentanediol, zinc pyrithione, chlorobutanol, isopropylmethylphenol, nonionic surfactant (polyoxyethylene lauryl ether, polyoxyethylene nonylphenyl ether, polyoxyethylene octyl phenyl ether, etc.) ), amphoteric surfactant, anionic surfactant (sodium lauryl sulfate, potassium lauric acid, etc.), cationic surfactant (brominated whale) Trimethylammonium chloride, ammonium benzyl chloride, benzyl ammonium chloride, gentian violet, formaldehyde, hexamine, bright green, malachite green, crystal violet, Jamal, photoreceptor 101 , Photosensitive No. 201, No. 401, N-long-chain mercapto basic amino acid derivatives and acid addition salts thereof, zinc oxide, big leaf yew, buckwheat, propolis and the like.

(29) Humectant

Glycerin, propylene glycol, 1,3-butanediol, hyaluronic acid or a salt thereof, polyethylene glycol, chondroitin sulfate or a salt thereof, a water-soluble chitin derivative, a water-soluble chitosan derivative, pyrrolidone carboxylic acid or Its sap of salt, sodium lactate, Mini sasanikishi (a Japanese organic rice) extract, natto metabolite, natto extract, loofah water, Betula platyphylla or Korean pine

(30) Elastase activity inhibitor

Diisopropyl fluorophosphate, plant extracts (Astragalus, young cut grass, Sophora flavescens, mulberry leaves, cinnamon, geranium, Kangfu grass, sage, western elder, linden, peony), seaweed extract, etc.

(31) head lice agent

Selenium disulfide, alkyl isoquinolinium bromide solution, zinc pyrithione, jenselid, xanthene, canthax, ginger, capsicum, quinine hydrochloride, strong ammonia, potassium hydrobromide, hydrobromine Sodium, thioglycolic acid, and the like.

(32) Anti-androgen agent

Follicle hormones (estrone, estradiol, ethinyl estradiol, etc.), isoflavones, aurora and other dragons.

(33) peripheral blood flow promoter

Vitamin E or its biological, surimi extract, garlic extract, ginseng extract, aloe extract, gentian extract, angelica extract, scutellaria, capperium chloride, minuridine and the like.

(34) Stimulant

Capsicum anthraquinone, vanillyl citrate, cantharidin, ginger, peppermint oil, l-menthol, camphor, benzyl nicotinic acid, and the like.

(35) Metabolic active agent

Photoreceptor No. 301, big leaf thuja, pantothenic acid or its organism, allantoin, placenta extract, biotin, fifteen glycerol carbonate and the like.

(36) anti-lipid leakage agent

Pyridoxine or its biological, sulfur, vitamin B6 and the like.

(37) Keratinolytic agent

Resorcinol, salicylic acid, lactic acid, urea, and the like.

(38) oxidant

Hydrogen peroxide water, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium hydrobromide, potassium hydrobromide, sodium pyrophosphate, orthophosphoric acid Sodium, sodium citrate hydrogen peroxide adduct, sodium sulfate hydrogen peroxide adduct, sodium chloride hydroperoxide adduct, beta-tyrosinase enzyme solution, mushroom extract, and the like.

(39) Depilatory agent

An inorganic reducing agent such as barium sulfate, sodium sulfide, barium sulfide or calcium sulfide, thioglycolic acid or a salt thereof (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate, Bismuth thioglycolate).

(40) Ranunculus swell

Ethanolamine, urea, hydrazine, and the like.

(41) Dyestuff

5-Amino o-cresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminopurine, 3,3'-Asia Aminodiphenol, 2,4-diaminophenoxyethanol hydrochloride, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitro-p-phenylenediamine hydrochloride, terephthalic acid hydrochloride Amine, N-phenyl-p-phenylenediamine hydrochloride, m-phenylenediamine hydrochloride, o-aminophenol, N-phenyl-p-phenylenediamine, 1,4-diaminopurine, 2,6-diamino Pyridic acid, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-diamine, nitro-p-phenylenediamine, p-aminophenol, p-nitro-o-phenylenediamine, pair Phenylenediamine, p-methylaminophenol, picrylic acid, sodium picrate, N,N'-bis(4-aminophenyl)-2,5-diamino-1,4-anthracene Amine, 5-(2-hydroxyethylamino)-2-methylphenol, N-phenyl-p-phenylenediamine, m-aminophenol, m-phenylenediamine, 5-amino-o-cresol sulfate, sulfuric acid 2 -Amino-5-nitrophenol, o-aminophenol sulfate, p-chlorophenyl p-phenylenediamine, 4,4'-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, toluene sulfate -2,5-diamine, nitro-p-phenylenediamine sulfate, amine-based sulfate Phenol, p-nitro-o-phenylenediamine sulfate, p-nitro-m-phenylenediamine sulfate, p-phenylenediamine sulfate, p-methylaminophenol sulfate, m-aminophenol sulfate, m-phenylenediamine sulfate, catechol, Diphenylamine, α-naphthol, hydroquinone, biphenyltriol, phloroglucinol, gallic acid, resorcinol, citric acid, 2-hydroxy-5-nitro-2', 4'-Diaminoazobenzene-5'-sulfonic acid sodium, hematoxylin and the like.

(42) Spice

Natural animal flavors such as musk, civet, beaver, and ambergris; fennel oil, white peony oil, perfume tree oil, iris oil, fennel oil, orange essential oil, canadian oil, gelatin oil, cardamom Essential oil, guaiac essential oil, cumin essential oil, fishing rod essential oil, cinnamon oil, cinnamon essential oil, geranium essential oil, Cuban essential oil, eucalyptus essential oil, perilla essential oil, red peony essential oil, citronella essential oil, jasmine essential oil, ginger grass essential oil, cedar Tree essential oil, spearmint essential oil, western mint essential oil, anise essential oil, tuberose essential oil, thong essential oil, orange blossom essential oil, winter green essential oil, balsam essential oil, scorpion peppermint essential oil, rose essential oil, geranium essential oil, eucalyptus Essential oil, eucalyptus essential oil, white sandalwood essential oil, lime essential oil, laurel essential oil, vetiver essential oil, bergamot essential oil, Peruvian essential oil, rosewood essential oil, eucalyptus essential oil, red orange essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, Aromatic essential oils such as agarwood essential oil, lemongrass essential oil, lemon essential oil, rose essential oil, and Japanese mint essential oil; other synthetic flavors.

(43) Pigments, colorants

Red cabbage pigment, red rice pigment, valerian pigment, annatto red pigment, cuttlefish melanin, scutellaria, sputum pigment, krill pigment, persimmon pigment, caramel, gold, silver, hazelnut pigment, corn pigment, onion pigment, Tamarind pigment, spirulina pigment, buckwheat whole grass pigment, cherry pigment, seaweed pigment, chrysanthemum pigment, grape juice pigment, marigold pigment, purpura pigment, purple hawthorn pigment, shellac pigment, rutin.

(44) Other

Can be listed as: hormones, metal ion chain sealers, pH adjusters, chelating agents, antiseptic, antifungal agents, cooling agents, stabilizers, emulsifiers, animal, plant proteins and their decomposition products, animal, plant polysaccharides And its decomposition products, animals, plant glycoproteins and their decomposition products, blood flow promoters, anti-inflammatory agents, anti-inflammatory agents, anti-allergic agents, wound treatment agents, foam boosters, tackifiers, enzymes, purified water (including Electronic water, small clustered water, etc.), oral preparations, deodorizing, deodorizing agents, bittering materials, seasonings, etc., by using these together, the effect of the present invention or other cosmetic effects can be expected to increase Or multiply and improve.

The other aspects of the present invention include the following.

A skin permanent bacteria balance improving agent, a Staphylococcus epidermidis proliferation promoting agent, a Staphylococcus aureus proliferation inhibitor, a P. acnes proliferation inhibitor, a glycerol production promoter of Staphylococcus epidermidis, or an antimicrobial peptide produced by epidermal cells Use of Lactobacillus paracasei MCC1849 (NITE BP-01633) in the manufacture of an accelerator.

A Lactobacillus paracasei used to improve the balance of skin bacteria, promote the proliferation of Staphylococcus epidermidis, inhibit the proliferation of Staphylococcus aureus, inhibit the proliferation of Propionibacterium acnes, promote the production of glycerol by Staphylococcus epidermidis, or promote the production of antimicrobial peptides by epidermal cells. MCC1849 (NITE BP-01633).

A Lactobacillus paracasei MCC1849 for improving the balance of skin bacteria, promoting the growth of Staphylococcus epidermidis, inhibiting the proliferation of Staphylococcus aureus, inhibiting the proliferation of Propionibacterium acnes, promoting the production of glycerol by Staphylococcus epidermidis, or promoting the production of antimicrobial peptides by epidermal cells. (NITE BP-01633).

Method for improving skin balance of common bacteria, method for promoting growth of Staphylococcus epidermidis, method for inhibiting growth of Staphylococcus aureus, method for inhibiting proliferation of Propionibacterium acnes, method for promoting glycerol production by Staphylococcus epidermidis, or production of antimicrobial peptide by epidermal cells The promotion method includes: applying Lactobacillus paracasei MCC1849 (NITE BP-01633) to a mammalian stage, or inhibiting the growth of the skin resident colony balance improving agent, the Staphylococcus epidermidis proliferation promoter, and the Staphylococcus aureus of the present invention. The agent, the P. acnes proliferation inhibitor, the glycerol production promoter of S. epidermidis, or the antibacterial peptide production promoter by epidermal cells is applied to a mammal.

Examples of the mammal include human, cow, horse, sheep, goat, pig, monkey, dog, cat, rat, mouse, hamster, guinea pig, and the like. It is preferably human.

Further, as another aspect of the present invention, the following contents can be mentioned.

Use of Lactobacillus paracasei MCC1849 (NITE BP-01633) in the manufacture of a skin protectant.

A Lactobacillus paracasei MCC1849 (NITE BP-01633) used to protect the skin.

A Lactobacillus paracasei MCC1849 (NITE BP-01633) for protecting the skin.

A method for protecting a skin comprising the step of applying Lactobacillus paracasei MCC1849 (NITE BP-01633) to a mammal or applying the skin protectant of the present invention to a mammal.

Examples of the mammal include human, cow, horse, sheep, goat, pig, monkey, dog, cat, rat, mouse, hamster, guinea pig, and the like. It is preferably human.

 [Examples]  

Hereinafter, examples of the Lactobacillus paracasei MCC1849 which uses the most remarkable effects of the present invention are shown, but the present invention is not limited to the examples. In the present embodiment, "%" means "% by mass" unless otherwise specified.

(microorganism deposit)

Lactobacillus paracasei MCC1849 was deposited in the Patent Microbiology Depository Center (Kazusa-Kamatari 2-5-8 122, Kisarazu, Chiba Prefecture, Japan) on June 6, 2013, and was deposited in 2013. On December 19th, it was transferred to the international deposit based on the Budapest Treaty. The commission number is NITE BP-01633.

Lactobacillus paracasei MCC1849 is not limited to the above-mentioned registered bacteria, and may be a lactic acid bacteria substantially equivalent to the above-mentioned registered bacteria. The lactic acid bacteria which are substantially equivalent to the above-mentioned deposited bacteria are lactic acid bacteria which belong to Lactobacillus paracasei and which promote the proliferation of Staphylococcus epidermidis, and inhibit the proliferation of Staphylococcus aureus and Propionibacterium acnes, and can improve the balance of the skin resident bacteria. It is a lactic acid bacterium that promotes the production of glycerol by Staphylococcus epidermidis, prevents dry skin, regulates the pH of the skin surface, inhibits the growth of bacteria, and promotes the production of human β-defensin-3 as an antibacterial peptide in skin epidermal keratinocytes. Further, the substantially identical lactic acid bacterium is further a base sequence of the 16S rRNA gene of the bacterium, and the base sequence of the 16S rRNA gene of the lactic acid bacterium is preferably 99.86% or more, further preferably 99.93% or more, and more preferably 100. % is identical, and is preferably a lactic acid bacterium having the same bacteriological properties as the above-mentioned registered bacteria.

(heat treatment of dead bacteria in Lactobacillus paracasei MCC1849)

Lactobacillus paracasei MCC1849 (NITE BP-01633) was purchased from Morinaga Dairy Co., Ltd.

(Lactobacillus casei ATCC334, Lactobacillus paracasei ATCCBBA-52, Lactobacillus sakei ATCC 15521, Lactobacillus plantarum ATCC 8014 heat treated dead cells)

Lactobacillus casei ATCC334, Lactobacillus paracasei ATCCBBA-52, Lactobacillus sphaeroides ATCC 15521, and Lactobacillus plantarum ATCC 8014 were purchased from Kanto Chemical. Each strain was cultured for 18 hours at 37 ° C using 1 L of MRS medium (Kanto Chemical). The cells were recovered by centrifugation at 3000 rpm for 20 minutes. The cells were washed twice with water and then dispersed in 100 mL of distilled water. After the cell dispersion was heated at 100 ° C for 10 minutes, the cells were collected by centrifugation, dried, and the resultant was subjected to heat treatment to treat dead cells.

(Manufacture of plant polysaccharides)

As a plant polysaccharide, a polysaccharide derived from Fira aloe is produced. 100 g of F. aloe leaves are pulverized, and then subjected to press extraction and filtration. After adding ethanol or isopropyl alcohol to the obtained filtrate and stirring, the resulting precipitate is recovered, and ethanol or isopropyl alcohol is added again for washing. The obtained washings were dried to obtain a Fira aloe polysaccharide powder.

(from natural polysaccharides)

Further, the above-mentioned heat treatment of the dead cells and the tamarind gum (DSP Gokyo Food & Chemical) which is a seed derived from tamarind as a polysaccharide, and the in vitro component of the microorganism derived from the microorganism are used. Xianjiao (DSP Wuxie Food and Chemistry) or Lancang (DSP Wuxie Food and Chemistry), cationized guar gum derived from legumes (DSP Wuxie Food and Chemistry).

(sample preparation 1)

Heated Lactobacillus paracasei MCC1849 with culture medium to heat the dead cells with Lactobacillus casei ATCC334, Lactobacillus paracasei ATCCBBA-52, Lactobacillus albicans ATCC 15521, Lactobacillus plantarum ATCC8014 in the form of the final concentration shown in Table 1. Each of the heat-treated dead cells was diluted to prepare Samples 1 to 7.

(sample preparation 2)

The heat-treated dead bacteria of Lactobacillus paracasei MCC1849 was mixed with Fira aloe polysaccharide, tamarind gum, tri-sac, gum, and cationized guar to prepare samples 8 to 19 (Table 1) shown in Table 2. Add the final concentration).

(sample preparation 3)

The heat-treated dead bacteria of Lactobacillus paracasei ATCCBBA-52 was mixed with Fira aloe polysaccharide, tamarind gum, tri-sac, gum, and cationized guar to prepare sample 20 to sample 31 shown in Table 3. (The medium is added to the final concentration).

(Cultivation of Staphylococcus epidermidis standard bacteria)

Nutri bouillon No. 2 (manufactured by Nissui Pharmaceutical Co., Ltd.) was used as a culture medium. Sample 1 to Sample 3 were added to the medium (Table 1 value: final concentration of the medium). Then, the Staphylococcus epidermidis standard strain (ATCC12228) was inoculated to the medium at a rate of 1 × 10 ³ CFU / μL to 5 × 10 ³ CFU / μL, and the cells were cultured at 37 ° C for 24 hours. Further, in the control group, it was assumed that the heat-treated dead cells (sample 1) were not added.

(Results of colony formation of Staphylococcus epidermidis standard bacteria)

The results are shown in Fig. 1. The colony was heat-treated by adding 0.1% to 0.5% of Lactobacillus paracasei MCC1849, and the colony formation of Staphylococcus epidermidis standard strain (ATCC12228) was increased by about 9 to 10 times with respect to the control group (Fig. 1).

(culture of Staphylococcus aureus standard bacteria)

Nutri bouillon No. 2 (manufactured by Nissui Pharmaceutical Co., Ltd.) was used as a culture medium. Sample 1 to Sample 3 were added to the medium (Table 1 value: final concentration of the medium). Then, Staphylococcus aureus standard bacteria (ATCC 6538) was inoculated to the medium at a rate of 1 × 10 ³ CFU / μL to 5 × 10 ³ CFU / μL, and the cells were cultured at 37 ° C for 24 hours. Further, in the control group, it was assumed that the heat-treated dead cells (sample 1) were not added.

(Results of colony formation of Staphylococcus aureus standard bacteria)

The results are shown in Figure 2. By adding 0.1% of Lactobacillus paracasei MCC1849 to heat-treat the dead cells, the colony formation of Staphylococcus aureus standard strain (ATCC6538) was reduced by about 2.8%, and by about 0.5%, it was reduced by about 7.5% (Fig. 2). ).

(Method of collecting bacteria from human skin)

The sterilized cotton swab was rubbed 3 times in the longitudinal direction 3 cm between the eyebrows of the subject, and rubbed 3 times in the transverse direction to collect the skin resident bacteria. Then, the cotton swab was washed in 10 mL of phosphate buffered physiological saline solution to collect bacteria.

(Cultivation of human skin resident bacteria)

Nutri bouillon No. 2 (manufactured by Nissui Pharmaceutical Co., Ltd.) was used as a culture medium. Sample 1 to Sample 3 were added to the medium (Table 1 value: final concentration of the medium). Then, the skin resident bacteria collected by the above method were inoculated to 100 μL of the medium. These were incubated at 37 ° C for 24 hours. Further, in the control group, it was assumed that the heat-treated dead cells (sample 1) were not added.

(Results of colony formation of Staphylococcus epidermidis)

The results are shown in Fig. 3. The heat-treated dead cells were added by adding 0.1% of Lactobacillus paracasei MCC1849, and the colony formation of human S. epidermidis was increased by about 5 times, and by 7 times to 8 times by addition of 0.5% (Fig. 3).

(Results of colony formation of P. acnes)

The results are shown in Fig. 4. The colony formation of P. acnes was reduced by about 15% by adding 0.1% of Lactobacillus paracasei MCC1849, and was reduced by about 25% by adding 0.5% (Fig. 4).

(The ratio of the number of colonies formed by Staphylococcus epidermidis and Propionibacterium acnes in human skin resident bacteria)

The results are shown in Fig. 5. The sum of the number of colony formations of Staphylococcus epidermidis and Propionibacterium acnes was set to 100, and the ratio of each bacteria was determined. By adding the heat-treated dead bacteria of Lactobacillus paracasei MCC1849 to the culture solution of the skin resident bacteria collected from the human eyebrow, the ratio of Staphylococcus epidermidis is dependent on the concentration, and the ratio of P. acnes is increased. The concentration is reduced depending on the concentration (Figure 5).

(Cultivation of Staphylococcus epidermidis standard bacteria)

Nutri bouillon No. 2 (manufactured by Nissui Pharmaceutical Co., Ltd.) was used as a culture medium. Sample 1, sample 2, sample 4 to sample 7 were added to the medium (Table 1 value: final concentration of the medium). Then, the Staphylococcus epidermidis standard strain (ATCC12228) was inoculated to the medium at a rate of 1 × 10 ³ CFU / μL to 5 × 10 ³ CFU / μL, and the cells were cultured at 37 ° C for 24 hours. Further, in the control group, it was assumed that the heat-treated dead cells (sample 1) were not added.

(Results of colony formation of Staphylococcus epidermidis standard bacteria)

The results are shown in Fig. 6. Compared with the heat-treated dead cells of Lactobacillus casei ATCC334, Lactobacillus paracasei ATCCBBA-52, Lactobacillus sphaeroides ATCC 15521, and Lactobacillus plantarum ATCC 8014, Lactobacillus paracasei MCC1849 heat-treated dead cells to make Staphylococcus epidermidis standard The colony formation of the bacteria (ATCC 12228) was significantly increased (Fig. 6). In particular, it was confirmed that the increase was about 3 times with respect to the same family of Lactobacillus paracasei ATCCBBA-52, and it was increased about 2 times with respect to Lactobacillus sphaeroides ATCC 15521.

(Measurement of glycerol amount)

Nutri bouillon No. 2 was used as a medium for the culture, and the dead cells were heat-treated by adding Lactobacillus paracasei MCC1849 in a concentration of 0.25 w/w%, 0.5 w/w%, and 1 w/w% in the medium. Then, the Staphylococcus epidermidis standard strain (ATCC12228) was inoculated to the medium at a rate of 1 × 10 ³ CFU / μL to 5 × 10 ³ CFU / μL, and the cells were cultured at 37 ° C for 24 hours. Further, in the control group, the dead cells (sample 1) were heat-treated without adding Lactobacillus paracasei MCC1849, and each culture solution was taken. The cultures were stirred using a vortex mixer and the amount of glycerol was determined by the usual method of glycerol analysis kit (Cayman Chemical).

(measurement result of glycerin amount)

The results are shown in Fig. 7. The dead cells were heat-treated by Lactobacillus paracasei MCC1849, and the glycerol production from the Staphylococcus epidermidis standard strain (ATCC12228) was significantly increased (Fig. 7). In the case where the final concentration of 1.0 w/w% was added to the medium, it was confirmed that about 4 times the glycerin production promoting effect was obtained.

(Measurement of glycerol amount)

Nutri bouillon No. 2 was used as the culture medium, and Lactobacillus paracasei MCC1849, Lactobacillus casei ATCC334, Lactobacillus paracasei ATCCBBA-52, Lactobacillus albicans ATCC 15521, Lactobacillus plantarum ATCC 8014 were added at a final concentration of 1.0%. The respective dead cells were heat treated. Then, the Staphylococcus epidermidis standard strain (ATCC12228) was inoculated to the medium at a rate of 1 × 10 ³ CFU / μL to 5 × 10 ³ CFU / μL, and the cells were cultured at 37 ° C for 24 hours. In addition, in the control group, it was assumed that the heat-treated dead cells were not added, and each culture solution was taken. The cultures were stirred using a vortex mixer and the amount of glycerol was determined by the usual method of glycerol analysis kit (Cayman Chemical).

(measurement result of glycerin amount)

The results are shown in Fig. 8. Compared with the heat-treated dead cells of Lactobacillus casei ATCC334, Lactobacillus paracasei ATCCBBA-52, Lactobacillus sphaeroides ATCC 15521, and Lactobacillus plantarum ATCC 8014, Lactobacillus paracasei MCC1849 heat-treated the dead cells to S. epidermidis. The glycerol production of the standard bacteria (ATCC 12228) was significantly increased (Fig. 8). When the dead cells were heat-treated by Lactobacillus paracasei MCC1849, it was confirmed that the glycerin production-promoting effect was about 5 times, but in the case of other dead cells, only a 1-fold to 2-fold-promoting effect was confirmed.

(Quantitative analysis of human β-defensin-3)

Human epidermal keratinocytes (manufactured by Kurabo Co., Ltd.) were cultured to subconfluent using a twenty-four-well plate using a medium for epidermal cells (Humedia KG2, manufactured by Kurabo Co., Ltd.). After the culture medium for differentiation induction of calcium was added to Humedia KG2 and further cultured at 37 ° C for 3 days in the presence of 5% CO ₂ , it was replaced with a new medium. The dead cells were heat-treated by adding Lactobacillus paracasei MCC1849 to the medium at a final concentration of 0.05 w/w% and 0.1 w/w%. As a positive control, Staphylococcus epidermidis standard bacteria were added so as to be about 1 × 10 ⁴ cells/mL. After 24 hours of culture, the culture supernatant was recovered. Quantification of human β-defensin-3 was performed using the Human BD-3 Standard ABTS ELISA Development Kit (PeproTech).

(Quantitative analysis results of human β-defensin-3)

The results are shown in Fig. 9. The amount of β-defensin-3 expressed from human epidermal keratinocytes was also increased by heat treatment of the dead cells by Lactobacillus paracasei MCC1849 (Fig. 9).

(Quantitative analysis of human β-defensin-3)

Human epidermal keratinocytes were cultured to sub-fusion using a 24-well plate in the same manner as described above using a culture medium for epidermal cells. After the culture medium for differentiation induction of calcium was added to Humedia KG2 and further cultured at 37 ° C for 3 days in the presence of 5% CO ₂ , it was replaced with a new medium. Lactobacillus paracasei MCC1849 was added to heat-treat the dead cells in the medium at a final concentration of 0.1 w/w%, and Lactobacillus casei ATCC334 and Lactobacillus paracasei were added at a final concentration of 0.1 w/w%. The dead cells of each of ATCCBBA-52, Lactobacillus sphaeroides ATCC 15521, and Lactobacillus plantarum ATCC 8014 were heat treated. After 24 hours of culture, the culture supernatant was recovered. Quantification of human β-defensin-3 was performed using the Human BD-3 Standard ABTS ELISA Development Kit (PeproTech).

(Quantitative analysis results of human β-defensin-3)

The results are shown in Fig. 10. Compared with the heat-treated dead cells of Lactobacillus casei ATCC334, Lactobacillus paracasei ATCCBBA-52, Lactobacillus albicans ATCC15521, and Lactobacillus plantarum ATCC8014, Lactobacillus paracasei MCC1849 heat-treated dead cells to make human epidermal keratin The expression level of β-defensin-3 in the cells was significantly increased (Fig. 10). In particular, up to 4.5 times the expression level was confirmed with respect to the same family of Lactobacillus paracasei ATCCBBA-52.

(culture of Staphylococcus epidermidis standard bacteria in the presence of plants or polysaccharides derived from microorganisms)

Nutri bouillon No. 2 (manufactured by Nissui Pharmaceutical Co., Ltd.) was used as a culture medium. Sample 9 to sample 19 were added to the medium (Table 2 value: final concentration of the medium). Then, the Staphylococcus epidermidis standard strain (ATCC12228) was inoculated to the medium at a rate of 1 × 10 ³ CFU / μL to 5 × 10 ³ CFU / μL, and the cells were cultured at 37 ° C for 24 hours. In addition, the control group was set to heat-treated dead cells (sample 8) to which Lactobacillus paracasei MCC1849 was not added.

(Results of colony formation of Staphylococcus epidermidis standard bacteria derived from plants or polysaccharides derived from microorganisms)

The results are shown in Fig. 11. The Staphylococcus epidermidis standard strain (ATCC12228) caused by heat treatment of the dead bacteria by Lactobacillus paracasei MCC1849 by using a combination of Fira aloe polysaccharide, tamarind gum, samba gum, yam gum, and cationized guar gum The colony formation was multiplied separately (Fig. 11).

(culture of Staphylococcus epidermidis standard bacteria in the presence of plants or polysaccharides derived from microorganisms)

Nutri bouillon No. 2 (manufactured by Nissui Pharmaceutical Co., Ltd.) was used as a culture medium. Sample 21 to sample 31 were added to the medium (Table 3 value: final concentration of the medium). Then, the Staphylococcus epidermidis standard strain (ATCC12228) was inoculated to the medium at a rate of 1 × 10 ³ CFU / μL to 5 × 10 ³ CFU / μL, and the cells were cultured at 37 ° C for 24 hours. Further, the control group was set to heat-treated dead cells (sample 20) to which Lactobacillus paracasei ATCCBBA-52 was not added.

(Results of colony formation of Staphylococcus epidermidis standard bacteria derived from plants or polysaccharides derived from microorganisms)

The results are shown in Fig. 12. The Staphylococcus epidermidis standard strain (ATCC12228) caused by the heat treatment of the dead bacteria by Lactobacillus paracasei ATCCBBA-52 by using a combination of Fira aloe polysaccharide, tamarind gum, Sanxian gum, ylang gum, and cationized guar gum The colony formation showed a tendency to enhance, respectively, but this was not a significant multiplication effect seen in the case of heat-treated dead bacteria of Lactobacillus paracasei MCC1849 (Fig. 12).

(Production Examples of Various External Agents for Skin Protection)

1. Sunscreen (O/W (oil-in-water; oil-in-water) cream type)

(oil phase department)

(water phase department)

(manufacturing method)

The oil phase portion and the water phase portion are separately heated to 70 ° C to be dissolved, and the aqueous phase portion is sufficiently dispersed in titanium dioxide, and the oil phase portion is added, and the emulsified particles are uniformly homogenized by a homomixer, and then degassed, filtered, and cooled. Containment. The cooling method is performed by a method of gradually cooling while stirring a refrigerant into a sleeve of a production tank, and a method of cooling using a heat exchanger.

2. Sunscreen lotion (W/O (water-in-oil; water-in-oil) type emulsion)

(oil phase department)

(water phase department)

Purified water set to 100 residual amount 1,3-butanediol 5.0%

(manufacturing method)

The oil phase portion and the water phase portion are separately heated to 60 ° C to be dissolved, and the oil phase portion is sufficiently dispersed with titanium dioxide and zinc oxide, and the aqueous phase portion is added, and the emulsified particles are uniformly homogenized by a homomixer, and then degassed and filtered. Cool and contain.

3. Tanning oil

(manufacturing method)

The fluorenone resin was added to the fluorenone oil to be dissolved, and then isopropyl myristate was added and stirred well, and then the remaining components were added and stirred.

4. Powder foundation

(powder)

(substrate)

(manufacturing method)

The powder components are mixed by using a mixer such as a high-speed flow type mixer, and fine particles are further produced by a pulverizer, and then the base material is added, and the mixture is sufficiently mixed using a vertical screw type mixer or the like. Further, after pulverization and granulation using a hammer mill or the like, compression molding is performed.

5. Dual-use foundation

(powder)

(substrate)

(other)

(manufacturing method)

The powder components are mixed by using a mixer such as a high-speed flow type mixer, and fine particles are further produced by a pulverizer, and then the base material is added, and the mixture is sufficiently mixed using a vertical screw type mixer or the like. Further, after pulverization and granulation using a hammer mill or the like, compression molding is performed.

6. Cream (W/O type)

(A phase)

(phase B)

(C phase)

(D phase)

Phenoxyethanol 0.4%

(E phase)

Titanium dioxide, glycerin, alumina 3.5%

(F phase)

Dimethyl decylated cerium oxide 1.0%

(G phase)

(H phase)

Spice 0.3%

(manufacturing method)

The raw material of the phase A was mixed under stirring, and the temperature was raised to 70 ° C to 75 ° C, and the phase B was added to the phase A while stirring, and stirred until it became uniform. In the other vessel, the raw materials of the phase C are mixed while stirring, and the temperature is raised to 70 ° C to 75 ° C. The phase C is added to the AB mixed phase, and the mixture is gradually added while stirring (about 5 minutes), and the phase C is added and stirred. Minutes, then cool naturally. When the temperature reached 40 ° C or lower, the phase D was added and stirred for 1 minute, the phase E was added and stirred for 5 minutes, then the phase F was added and stirred for 1 minute, and the phase G was added and stirred for 1 minute. The H phase was finally added and stirred for 2 minutes and then naturally cooled to room temperature.

7. Moisturizing gel (cosmetic liquid)

(A phase)

(phase B)

Sanxianjiao 0.1%

(C phase)

(manufacturing method)

The phase A and the phase B are separately mixed and prepared. By dispersing Sanxian gum and sodium polyacrylate by using a batch type disperser or the like, phase A is added to phase B at 75 ° C, and after a few minutes of homogenization, it is cooled while stirring slowly, and C which has become 40 ° C is added. The phase (menthol is pre-dissolved in ethanol).

8. Lipstick

(A phase)

(phase B)

Isopropyl myristate 10.0%

(C phase)

(D phase)

(E phase)

(manufacturing method)

The phase A is uniformly premixed, added to the phase B and uniformly mixed, and the phase C is further added and heated to 78 ° C to 80 ° C while stirring, and the phase D and the phase E are added in small portions, uniformly stirred, and then flowed into the mold. Cool naturally to room temperature.

9. Foundation (O/W type)

(oil phase)

(water box)

(powder)

(other)

Spice

(manufacturing method)

After the powder was pulverized, the aqueous phase was added and mixed, and the oil phase and the flavor were added thereto while stirring, and the mixture was emulsified and dispersed by a homomixer, and then cooled to room temperature.

10. Foundation cream (W/O type)

(oil phase)

(water box)

(powder)

(other)

Spice

(manufacturing method)

After pulverizing the powder, the aqueous phase was added and mixed, and the oil phase and the flavor were added thereto while stirring, and the mixture was emulsified and dispersed by a homomixer, and then cooled to room temperature.

11. Moisturizing cream (O/W type)

(A phase)

(phase B)

(C phase)

(manufacturing method)

After heating the A phase and the B phase to 70 ° C in different containers, the phase A was added to the phase B, homogenized using a batch type disperser or the like, and naturally cooled to 40 ° C while stirring. Then, phase C was added thereto and further homogenized, and cooled to room temperature.

12. Skin cream (quasi-drug)

(A phase)

(phase B)

(C phase)

(Method of manufacturing skin cream)

The phase A was dissolved while stirring. The phase B was heated while stirring to 50 ° C and dissolved. The phase C was heated while stirring to 50 ° C while stirring. While stirring the phase A, the phase B was added and emulsified at 50 ° C (phase D). The phase C was added while stirring the D phase and dissolved. The product was made by cooling to room temperature.

13. Moisturizing cream moisturizing test

(subject)

Seventeen people who met the following items were included as subjects. The average age is 42.0 years ± 10.7 years.

1) Japanese women aged 20 or older and 60 years old who have obtained the consent of the test.

2) Those who feel dry skin.

3) Concerned about the pigmentation and dullness of the skin.

4) It is not easy to apply makeup and easy to remove makeup.

5) During the test period, the commonly used facial cleansing foam and cream can be changed to the test article.

6) Regarding skin care during the test period, you can agree to use only the commonly used lotion and test cream, and stop using other creams, lotions, gels, beauty lotions, masks, etc.

In addition, the following are excluded from the subject.

1) Those who are pregnant or may be pregnant, and those who are breast-feeding.

2) Those who are allergic to cosmetics.

3) Those who have experience in cosmetic medicine that affects the part being tested.

4) Participate in other clinical trials.

In addition, as a matter of subject management, the following items are set.

1) Guarantee more than 6 hours of sleep on the day before the test.

2) Avoid outdoor leisure activities during the test period, and take measures such as hats and umbrellas to prevent sun exposure.

3) The facial cleansing foam and lotion which are usually used are changed to test articles during the test period.

4) Regarding the skin care during the test period, only the commonly used lotion and test cream are used, and other creams, lotions, gels, lotions, masks, etc. are discontinued.

5) Stimulants that avoid the effects of diet on skin condition during the test period.

6) Special care such as Facial Esthetique or supplements for beauty purposes are prohibited during the test period.

(Ingredients for facial cleansing foam)

(Method of manufacturing facial foam)

The potassium soap base was finely ground. After ironing with boiling water, dissolve and mix other ingredients.

(component of moisturizing cream (test article))

(A phase)

(phase B)

Phenoxyethanol 1.0000% behenyl alcohol 1.0000%

(C phase)

Hydrogenated lysolecithin 0.2000%

(D phase)

(E phase)

Lactobacillus paracasei MCC1849 heat treatment of dead cells 0.05% or 0.1%

(F phase)

(Manufacturing method of moisturizing cream (test article))

The phase A was mixed and dissolved by heating at 80 ° C to 85 ° C. After the phase B is dissolved, the phase C is added, and the mixture is heated and dissolved at 45 to 50 ° C, and the phases are separately added to the phase D, and the mixture is stirred until the phase E is uniformly dispersed. Then, it is heated to 80 ° C to 85 ° C. The phase B was stirred while being heated while being heated by a homomixer, and the phase A was added thereto to carry out emulsification. Then, cooling was started while stirring at a low speed, and the F phase was separately introduced in a stage of reaching 60 ° C or lower, and after uniformly dispersing, cooling and low-speed stirring were continued until 30 ° C was reached.

(the composition of moisturizing cream (control))

In the components of the moisturizing cream (test article), the components of the moisturizing cream (test article) were the same as those of the moisturizing cream (test article) except that the dead bacteria were heat-treated without adding Lactobacillus paracasei MCC1849.

(Manufacturing method of moisturizing cream (control))

It is the same as the manufacturing method of the above moisturizing cream (test article).

(experiment method)

The subject was allowed to use the control one week before using the test article, and then the test article was used for four weeks. Further, when the test subject used the test article, 0.5 g of the test article was applied to the face after washing the face with the above-mentioned facial cleansing foam. In either case, the test article is applied once in the evening.

The skin moisture content of the subject was measured after one week of using the control and four weeks after the use of the test article. The subject was washed before the measurement and domesticated in a constant temperature and humidity chamber for 20 minutes. Then, in the room, the skin water content (arbitrary unit; au) was measured using a Corneometer CM825 (Courage+Khazaka) at each of the right cheek and the left cheek, and the right cheek and the left cheek were calculated. The average of the measured values. The measurement is carried out in accordance with the product guidelines. The results are shown in Table 4. In addition, each value is the mean value ± standard deviation of the skin moisture content of the subject (17 persons).

According to Table 4, the skin moisture content of the subject after four weeks of use of the test article was significantly greater than in the case of using the control.

(industry availability)

The Lactobacillus paracasei MCC1849 of the present invention heat-treats the dead cells on the skin to promote the proliferation of Staphylococcus epidermidis which is a useful skin resident, and inhibits the proliferation of Staphylococcus aureus or Propionibacterium acnes as a harmful bacteria. Further, in a preferred embodiment, the production of glycerol derived from Staphylococcus epidermidis is promoted. Further, the production of antimicrobial peptides derived from epidermal cells is also promoted. Therefore, the use of a cosmetic material or a skin protectant which is normal or maintains normal skin condition is extremely high.

【Biomaterial Storage】

Domestic registration information [please note according to the registration authority, date, number order]

no.

Foreign deposit information [please note according to the country, organization, date, number order]

Lactobacillus paracasei MCC1849, Japan, the patent microbiology deposit center of the independent administrative agency product evaluation technology infrastructure (Kazusa-Kamatari 2-5-8 122, Kisarazu, Chiba Prefecture, Japan), deposited on June 6, 2013 and 2013 Transferred to the international deposit based on the Budapest Treaty on December 19, the commission number is NITE BP-01633.

Claims (8)

 A skin permanent bacteria balance improving agent is Lactobacillus paracasei MCC1849, that is, NITE BP-01633 as an active ingredient.    A skin protectant is Lactobacillus paracasei MCC1849, that is, NITE BP-01633 as an active ingredient.    The skin protectant as claimed in claim 2 is a Staphylococcus epidermidis proliferation promoter.    The skin protectant according to claim 2 is a Staphylococcus aureus proliferation inhibitor.    The skin protectant as claimed in claim 2 is a P. acnes proliferation inhibitor.    The skin protectant according to claim 2 is a glycerin production promoter of Staphylococcus epidermidis.    The skin protectant according to claim 2 is an antibacterial peptide production promoter caused by epidermal cells.    A cosmetic material comprising Lactobacillus paracasei MCC1849, also known as NITE BP-01633.