JPWO2018216744A1 - Cosmetics and skin protectants containing lactic acid bacteria - Google Patents

Abstract

An object of the present invention is to provide a cosmetic and a skin protective agent capable of improving the balance of skin indigenous bacteria, and preferably increasing the proportion of useful bacteria, Staphylococcus epidermidis, and Lactobacillus paracasei. The problem is solved by a skin indigenous bacteria balance improving agent or the like containing MCC1849 (NITE BP-01633) as an active ingredient.

Description

The present invention improves the skin indigenous bacteria balance, that is, by increasing the ratio of Staphylococcus epidermidis, which is a useful bacterium, a cosmetic that protects the skin from external stress and harmful bacteria such as dryness, and It relates to a skin protectant.

Humans maintain the living environment with various symbiotic microorganisms. A representative of symbiotic microorganisms is intestinal bacteria. Among the intestinal bacteria, lactic acid bacteria are known as useful bacteria, but their role is not limited to intestinal regulation but produces useful components such as organic acids by utilizing dietary fiber and the like, and It is reported that it also works to improve the function. Further, it has been reported that polysaccharides such as lipoteichoic acid possessed by lactic acid bacteria promote the formation of intestinal barrier, and can also act for the prevention and amelioration of inflammatory bowel diseases such as colitis. Among the intestinal bacteria, there are harmful bacteria such as Clostridium perfringens and Escherichia coli that produce toxins, but these harmful bacteria are also essential for the constant maintenance of anti-inflammatory immunocompetent cells, In recent years, attention has been focused on a component that works to improve the balance between useful bacteria and harmful bacteria, rather than a component intended to remove harmful bacteria (Non-Patent Document 1).

In the intestinal tract, useful bacteria such as lactic acid bacteria and bifidobacteria are ingested in order to improve the intestinal environment.In general, these useful bacteria are selected to select an acid-resistant strain in order to reach and colonize the intestine alive. Or enteric-type encapsulation was necessary. Further, in order to increase useful bacteria in the intestine, a method of ingesting foods and drinks containing dietary fiber and various oligosaccharides selectively assimilated to useful bacteria has been used. However, these methods may cause excessive intestinal fermentation or diarrhea depending on the constitution of the individual. As one method for solving such a problem, a method using heat-treated killed cells of lactobacillus has been studied.

Heat-treated killed cells of Lactobacillus paracasei, which is a type of lactic acid bacterium useful as an intestinal bacterium, induces the production of interleukin-12 (IL-12), which is an anti-inflammatory cytokine, It is known that the internal protection function can be improved. In addition, dead cells are easy to process as foods and drinks, and since unnecessary fermentation does not proceed, there is little influence on the storage stability of foods and drinks.

It is known that resident symbiotic microorganisms form a group in the skin as well as in the intestine. The role of these commensal microorganisms on the skin is similar to that of intestinal bacteria in the intestinal tract. A group of skin symbiotic microorganisms is called skin resident bacteria, and is mainly composed of Staphylococcus epidermidis, Staphylococcus aureus, Propionibacterium acnes, Corynebacterium, and the like. In order to maintain the protective function of the skin from the outside world, these skin-resident bacteria are usually maintained in a certain proportion of balance.

An example of this balanced tablet breakage is that the proportion of Staphylococcus aureus is increasing in the skin of patients with atopic dermatitis. In this patient, S. aureus is also known to produce toxins, causing further deterioration of skin conditions.

Even in healthy people, skin-resident bacteria are caused by environmental factors such as abrupt temperature changes, dryness, strong cleaning agents and drugs, psychosomatic factors such as trauma, mental stress, or poor general condition due to illness. It is known that the plexus balance is lost and the protective function is reduced (Non-Patent Document 2).

One of the useful ways to balance the indigenous skin balance is to increase the proportion of S. epidermidis. Staphylococcus epidermidis plays a role of physical protective functions such as decomposing sebum to produce glycerol and organic acid, preventing dry skin, and controlling the pH of the skin surface to suppress the growth of various bacteria. .. Therefore, when the proportion of Staphylococcus epidermidis decreases, these protective functions are not exerted, which may cause skin diseases. In order to prevent such skin diseases, moisturizers, antibacterial agents, bactericidal components, etc. have been widely used for external preparations for skin, but depending on these drug components, there is a concern that even the protective function by Staphylococcus epidermidis may be suppressed. there were.

Furthermore, Staphylococcus epidermidis also plays a role of stimulating skin epidermal keratinocytes, promoting production of antibacterial peptides from skin epidermal keratinocytes, and strengthening the biological protective function of the living body. Human β-defensin-3 is known as an antibacterial peptide produced from skin epidermal keratinocytes, and these antibacterial peptides are effective against Staphylococcus aureus which is a harmful bacterium of the skin. It has been reported that it exhibits an antibacterial action and acts as a biological barrier to protect the skin from harmful bacteria (Non-patent Document 3).

Conventionally, sugars such as oligosaccharides have been used as an assimilation source for the purpose of promoting the growth of S. epidermidis (Patent Document 1), but not only useful S. epidermidis, but also S. aureus In some cases, the skin could not be protected because it showed an assimilation ability against harmful bacteria. When sugar is applied to the skin, it often gives a sticky feeling, which causes another problem that it is difficult to formulate the feel.

JP 2007-0777121A

Satoshi Yamura, Effect of intestinal flora on immune system, Medical Probiotics Synergy, Inc., P107-112 (2009) Gallo, R.M. L. Et al., Journal of Investigative Dermatology 131, P1974-1980 (2011). Kisich, K.; O. Et al., Journal of Investigative Dermatology 127:2368-2380 (2007).

The problem to be solved by the present invention is to provide a cosmetic, a skin protector, or the like that can improve the balance of indigenous bacteria of the skin, and preferably increase the proportion of useful bacteria, Staphylococcus epidermidis.

The present inventors have investigated various lactic acid bacteria, and as a result of extensive studies, the effect of promoting the growth of Staphylococcus epidermidis on heat-treated killed cells of Lactobacillus paracasei MCC1849, which is a lactobacillus, and Staphylococcus aureus And it was found that it has an effect of suppressing the growth of Acne bacteria. It was also found that it has an effect of promoting the production of glycerol derived from Staphylococcus epidermidis and an effect of promoting the production of antimicrobial peptides derived from epidermal keratinocytes. It was found that the skin indigenous bacteria balance can be improved through any one of these effects or a series of functions. Furthermore, by combining heat-treated killed cells of Lactobacillus paracasei MCC1849 with aloe vera-derived, tamarind-derived, guar-seed-derived polysaccharides as plant-derived polysaccharides, and xanthan gum and gellan gum as microorganism-derived polysaccharides, epidermal grapes can be obtained. It was found that the growth effect of cocci enhances synergistically.

The present invention provides a skin indigenous bacteria balance improving agent containing Lactobacillus paracasei MCC1849 (NITE BP-01633) as an active ingredient.
The present invention also provides a skin protective agent containing Lactobacillus paracasei MCC1849 (NITE BP-01633) as an active ingredient.
The skin protective agent is preferably Staphylococcus epidermidis growth promoter, Staphylococcus aureus growth inhibitor, acne growth inhibitor, glycerol production promoter by Staphylococcus epidermidis, or antimicrobial peptide production promoter by epidermal cells It is in the form.
The present invention also provides a cosmetic containing Lactobacillus paracasei MCC1849 (NITE BP-01633).

The other forms of the skin indigenous bacteria balance improving agent, the skin protecting agent, and the cosmetic further contain a plant polysaccharide and/or a microorganism-derived polysaccharide as an active ingredient.

The cosmetics and skin protectants according to the present invention can promote the growth of Staphylococcus epidermidis and suppress the growth of Staphylococcus aureus and Acne bacterium to balance the skin indigenous bacteria. Furthermore, in a preferred form, the cosmetic or skin protectant according to the present invention promotes the production of glycerol by Staphylococcus epidermidis, prevents dry skin, regulates the pH of the surface of the skin and suppresses the growth of germs, and It is possible to promote the production of human β-defensin-3 which is an antibacterial peptide in skin epidermal keratinocytes. The cosmetic and skin protectant according to the present invention can protect the skin from external stress such as dryness and harmful bacteria through any one or more of these effects.

The results obtained by adding the heat-treated killed cells of Lactobacillus paracasei MCC1849 to Staphylococcus epidermidis standard bacteria (ATCC 12228) and measuring the number of colonies formed by the culture method are shown. Heat-killed killed cells of Lactobacillus paracasei MCC1849 were added to Staphylococcus aureus standard bacteria (ATCC6538), and the results of measuring the number of colonies formed by the culture method are shown. The result of measuring the colony formation number of Staphylococcus epidermidis by adding heat-treated killed cells of Lactobacillus paracasei MCC1849 to a culture solution of skin-resident bacteria collected from a subject (human eyebrow) is shown. The heat-treated killed cells of Lactobacillus paracasei MCC1849 were added to the culture solution of skin-resident bacteria collected from a subject (human eyebrow), and the number of colony-forming acne bacteria was measured. Heat-treated killed cells of Lactobacillus paracasei MCC1849 were added to a culture solution of skin-resident bacteria collected from a subject (human eyebrow), and the colony formation number of Staphylococcus epidermidis and Acne bacterium was measured, and the ratio was calculated. The calculated results are shown. The heat-killed killed cells of the genus Lactobacillus are added to Staphylococcus epidermidis standard bacteria (ATCC 12228), and the number of colonies formed is measured by a culture method. Heat-killed killed cells of Lactobacillus paracasei MCC1849 were added to the culture medium of a standard strain of Staphylococcus epidermidis (ATCC 12228), and the result of quantifying the amount of glycerol is shown. The heat-treated killed bacterium of the genus Lactobacillus is added to the culture solution of Staphylococcus epidermidis standard bacteria (ATCC 12228), and the result of quantifying the amount of glycerol is shown. Lactobacillus paracasei MCC1849 heat-treated human normal keratinized cell culture strain was cultured in a medium containing killed cells for 24 hours, and the amount of human β-defensin-3 (hBD3) in the culture supernatant was measured by ELISA. The measurement result is shown. A human normal keratinized cell culture strain was cultured for 24 hours in a medium containing heat-treated killed Lactobacillus cells, and the amount of human β-defensin-3 (hBD3) in the culture supernatant was measured by an ELISA method. Represents the result. Lactobacillus paracasei MCC1849 heat-treated killed cells and a sample in which aloe vera polysaccharide, tamarind gum, xanthan gum, gellan gum, and cationized guar gum were used in combination, were added to Staphylococcus epidermidis standard bacteria (ATCC 12228), and colonies were cultured. The result of having measured the number of formation is shown. Lactobacillus paracasei ATCCBBA-52 heat-treated killed cells, and a sample in which Aloe vera polysaccharide, tamarind gum, xanthan gum, gellan gum, and cationized guar gum were used in combination, were added to Staphylococcus epidermidis standard bacteria (ATCC 12228), and the culture method was used. The results obtained by measuring the number of colonies formed are shown below.

Cosmetics of the present invention, or skin indigenous bacteria balance improver, Staphylococcus epidermidis growth promoter, Staphylococcus aureus growth inhibitor, Acne bacteria growth inhibitor, glycerol production promoter by Staphylococcus epidermidis, antimicrobial peptide production by epidermal cells Each skin protective agent such as an accelerating agent (in the present specification, these may be referred to as “cosmetic or skin protective agent”, “cosmetic and skin protective agent”, “cosmetic or skin protective agent”, etc.) Contains Lactobacillus paracasei MCC1849 as an active ingredient. In a preferred embodiment, the Lactobacillus paracasei MCC1849 lactobacillus is a heat-treated killed bacterium.
The skin protectant of the present invention contains Lactobacillus paracasei MCC1849 as an active ingredient, and does not prevent inclusion of other ingredients. That is, the skin protective agent of the present invention is equivalent to the skin protective composition. Therefore, the skin protective agent of the present invention can be read as a skin protective composition. Further, the composition is a concept including a mixture, and it does not matter whether the components of the composition are uniform or non-uniform. This means that the skin indigenous bacteria balance improver, Staphylococcus epidermidis growth promoter, Staphylococcus aureus growth inhibitor, Acne bacteria growth inhibitor, glycerol production promoter by Staphylococcus epidermidis, antibacterial peptide production promoter by epidermal cells, etc. Is also the same.

The cosmetic or skin protectant of the present invention improves the skin indigenous bacteria balance. The skin indigenous bacteria balance refers to a balance between Staphylococcus epidermidis and harmful bacteria among skin indigenous bacteria. Examples of harmful bacteria include Staphylococcus aureus and acne. Improving the skin indigenous bacteria balance means increasing the ratio of the number of Staphylococcus epidermidis to the number of harmful bacteria. The improvement of the skin indigenous bacteria balance is preferably growth of Staphylococcus epidermidis, suppression of harmful bacteria, or both.
Furthermore, the cosmetic or skin protectant of the present invention preferably has the following characteristics.
1. It promotes the production of glycerol by Staphylococcus epidermidis, thereby preventing dry skin and regulating the pH of the surface of the skin to suppress the growth of germs.
2. It promotes the production of antibacterial peptides against harmful bacteria such as Staphylococcus aureus by epidermal keratinocytes.
Furthermore, when used in combination with Lactobacillus paracasei MCC1849 in combination with plant-derived polysaccharides such as aloe vera, polysaccharides derived from seaweed, and polysaccharides derived from microorganisms, the growth of Staphylococcus epidermidis synergistically occurs. Facilitate.
The cosmetic or skin protectant of the present invention preferably enhances the function of protecting the skin from external stress such as dryness and harmful bacteria, through a series of functions of any one or two or more of the above. You can

As described above, the cosmetic and skin protectant of the present invention can improve the skin indigenous bacteria balance. The action accompanying the improvement of the skin indigenous bacteria balance or the action leading thereto is presumed as follows.
When a composition containing Lactobacillus paracasei MCC1849 is applied to the skin, the same bacteria promote the growth of Staphylococcus epidermidis. Staphylococcus epidermidis produces glycerol and short-chain fatty acids and promotes the production of antimicrobial peptides by skin cells. In addition, Lactobacillus paracasei MCC1849 acts directly on skin cells to promote the production of antimicrobial peptides by skin cells. Glycerol improves the moisturizing properties of the skin. Short-chain fatty acids lead to weak acidity of the skin, promote the growth of Staphylococcus epidermidis, and suppress the growth of harmful bacteria such as Staphylococcus aureus and acne. It is possible to reduce the destruction of the skin barrier due to harmful bacteria and suppress the deterioration of the moisturizing property of the skin. Therefore, the cosmetics and skin protectant of the present invention are for promoting the growth of Staphylococcus epidermidis, suppressing the growth of harmful bacteria such as Staphylococcus aureus and acne, and promoting the production of glycerol and/or short chain fatty acids by Staphylococcus epidermidis. It can also be used for any one or two or more purposes of promoting the production of antimicrobial peptides by skin cells and moisturizing the skin. However, except for the effects shown by the examples, the above effects are speculative, and the effects of improving the skin indigenous bacteria balance of the cosmetics and skin protectants of the present invention are limited to those based on the above effects. It is not intended to be.
Further, since the present invention has, for example, a moisturizing property, it is considered that the present invention can be used, for example, for the prevention or treatment of diseases or conditions that can be prevented or treated by moisturizing in mammals. “Treatment” also includes amelioration. Specific examples of its application include xerosis, atopic dermatitis (eczema), dry dermatitis, psoriasis and the like. Examples of mammals include humans, cows, horses, goats, sheep, pigs, monkeys, dogs, cats, rats, mice, hamsters, guinea pigs, and the like.

As a method for culturing Lactobacillus paracasei MCC1849, conventionally used methods for culturing lactic acid bacteria can be appropriately modified and used, including conventionally known methods for culturing lactic acid bacteria. For example, the culture temperature may be 25-50°C, preferably 35-42°C. The culture may be carried out under either aerobic conditions or anaerobic conditions, but it is preferable to carry out the cultivation under anaerobic conditions. For example, the cultivation can be carried out while aerating an anaerobic gas such as carbon dioxide gas. Moreover, you may culture|cultivate under microaerobic conditions, such as liquid stationary culture.

The medium for culturing Lactobacillus paracasei MCC1849 is not particularly limited, and a medium usually used for culturing lactic acid bacteria can be appropriately modified and used as necessary. That is, as the carbon source, for example, saccharides such as galactose, glucose, fructose, mannose, cellobiose, maltose, lactose, sucrose, trehalose, starch, hydrolyzed starch, molasses and the like can be used depending on the assimilability. As the nitrogen source, for example, ammonia, ammonium salts such as ammonium sulfate, ammonium chloride, ammonium nitrate and nitrates can be used. As the inorganic salts, for example, sodium chloride, potassium chloride, potassium phosphate, magnesium sulfate, calcium chloride, calcium nitrate, manganese chloride, ferrous sulfate, etc. can be used. Further, organic components such as peptone, soybean powder, defatted soybean meal, meat extract, yeast extract and the like may be used. Further, as the prepared medium, for example, MRS medium can be preferably used.

The heat-treated killed cells of Lactobacillus paracasei MCC1849 can be produced by the following method. From the culture obtained by culturing Lactobacillus paracasei MCC1849 according to a conventional method, for example, cells are collected by a method such as filtration and centrifugation, washed with water, suspended in water or the like, and heat-treated. Further, it can be prepared by concentrating and drying, if necessary. It may be carried out using a commonly used spray dryer, freeze dryer or the like. Depending on the case, before or after the sterilization treatment by heating or the like, or before or after the drying treatment, an enzyme treatment, a surfactant treatment, a grinding/pulverization treatment can be carried out, and those obtained by these treatments can also be used in the present invention. Included in dead cells.

The dead cells of Lactobacillus paracasei MCC1849 of the present invention are not limited to the heat-treated dead cells. The dead cells of Lactobacillus paracasei MCC1849 of the present invention are generally those that are incapable of growth even when cultured under suitable culture conditions and may not be metabolically active. , Generally, even when cultivated under suitable culture conditions, it is difficult to grow, but the metabolic activity of the bacterium is lower than that of the live bacterium, but the bacterium does not show metabolic activity. It includes injured bacteria in a state of having significant activity as compared with the body. Examples of killed cells of Lactobacillus paracasei MCC1849 of the present invention include killed cells produced by chemical treatment (chemical solution sterilization method, ethylene oxide gas sterilization method, hydrogen peroxide gas plasma sterilization method, etc.) Treatment (flame sterilization method, dry heat sterilization method, ultra high temperature heat treatment method, high pressure steam sterilization method, high frequency sterilization method, radiation sterilization method (γ ray sterilization method, X ray sterilization method, etc.), electron beam sterilization method, ultraviolet sterilization method , Filter sterilization method, ultrasonic crushing method, pressure crushing method, etc.).

The water used for washing and suspension includes pure water, tap water, well water, mineral water, mineral water, hot spring water, spring water, fresh water and the like, as well as those which have been subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering and the like. Therefore, the water usable in the present invention includes purified water, heat-treated water, ion-exchanged water, physiological saline, various buffer solutions, and the like.

The suspension of heat-treated killed cells of Lactobacillus paracasei MCC1849 obtained as described above is a skin indigenous bacteria balance improving agent, a Staphylococcus epidermidis growth promoter, a Staphylococcus aureus growth inhibitor, and an acne bacterium growth inhibitor. As a skin protective agent such as an agent, a glycerol production promoter by Staphylococcus epidermidis, an antibacterial peptide production promoter by epidermal cells, etc., and skin protection that maintains the health of the skin surface condition through any or a series of these functions. It can be used as cosmetics.

The skin indigenous bacteria balance improving agent according to the present invention, Staphylococcus epidermidis growth promoter, Staphylococcus aureus growth inhibitor, Acne bacteria growth inhibitor, glycerol production promoter by Staphylococcus epidermidis, antimicrobial peptide production promoter by epidermal cells It is preferable that the heat-treated killed bacterial cell is prepared as a suspension so as to be contained in the range of 1×10 ⁶ to 1×10 ¹² cells/g. Further, it is more preferable that the suspension is prepared so as to be contained in the range of 1×10 ⁷ to 1×10 ¹¹ cells/g. In addition, in the case of cosmetics that are used on a daily basis, considering that the total amount per use is usually about 0.5 g, the heat-treated killed cells are preferably 1 It is preferably formulated so that it is contained within a range of 1/3 to 1×10 ⁶ to 1×10 ¹² cells/g. 1×10 ⁷ to 1×10 ¹¹ cells It is more preferable that it is blended and prepared so as to be contained within a range of 1/3 to 1 times the amount of /g. In addition, in the killed bacterial cells of the example, 1 μg corresponds to 3.75×10 ⁶ cells, and thus can be converted by this value.

After culturing, Lactobacillus paracasei MCC1849 may be used as it is, or may be diluted or concentrated, or may be bacterial cells recovered from the culture. In addition, as shown in Examples, heat-treated killed cells of Lactobacillus paracasei MCC1849 have an effect of improving skin-indigenous bacterial balance, an effect of promoting Staphylococcus epidermidis, an effect of suppressing Staphylococcus aureus growth, and an effect of suppressing Acne bacterium growth, It has a skin protecting action such as a glycerol production promoting action by S. epidermidis and an antibacterial peptide production promoting action by epidermal cells. Therefore, it is considered that even viable bacteria similarly exhibit the above skin protecting action. That is, in the present invention, the bacterium of Lactobacillus paracasei MCC1849 may be a live bacterium or a dead bacterium, or may be both a live bacterium and a dead bacterium. The killed bacteria may be a disrupted product of killed cells or a fraction thereof having the above-mentioned action.
In the present invention, when a live bacterium of Lactobacillus paracasei MCC1849 is used, 1×10 ⁶ to 1×10 ¹² CFU/g or 1×10 ⁶ to 1×10 ¹² CFU/mL (CFU: Colony Forming Unit( It is preferable that the suspension is prepared so as to be contained within the range of colony forming unit)). Further, it is more preferable that the suspension is prepared so as to be contained in the range of 1×10 ⁷ to 1×10 ¹¹ CFU/g or 1×10 ⁷ to 1×10 ¹¹ CFU/mL. In the case of killed bacteria, cfu/g or cfu/ml can be replaced with individual cells/g or individual cells/ml.

(Vegetable polysaccharides, seaweed-derived polysaccharides, microbial-derived polysaccharides)
In the cosmetic or skin protectant of the present invention, one or more selected from the group consisting of plant polysaccharides, seaweed-derived polysaccharides, and microorganism-derived polysaccharides may be used in combination with Lactobacillus paracasei MCC1849. Examples of the plant polysaccharide include polysaccharides of plants belonging to the genus Aloe. The aloe plants include Aloe barbadensis Miller, Aloe arborescen Miller var. Natalensis Berger, Aloe ferrospila al aire al-a al aire al-a al afera alfa, aloe afriana mirror. Baker) and the like.

The plant polysaccharide used in the cosmetic or skin protectant of the present invention is preferably a fraction containing the polysaccharide of the plant, and as a fraction fractionated using the whole plant. Although it may be used, it is preferably used as a fractionated product obtained by fractionating mesophyll (transparent gel portion). Such a plant or a part thereof is preferably crushed using a homogenizer or the like, squeezed if necessary and filtered to be liquefied, and obtained by extraction/washing with an organic solvent or hot water. The obtained fraction can be used as the plant polysaccharide used in the present invention.

In addition, the plant-derived polysaccharide-containing fraction used in the present invention, in addition to the plant-derived polysaccharide, within a range that does not affect the effects of the present invention, other components contained in the plant It may be included. For example, when it is derived from a plant belonging to the genus Aloe, sterols, anthraquinones, and the like are exemplified in addition to the polysaccharides contained in the plant belonging to the genus Aloe.

Furthermore, examples of the plant-derived polysaccharide include tamarind gum and cationized guar gum. Examples of polysaccharides derived from seaweed include alginic acid, fucodine, ascofiran, and porphyran. Examples of the polysaccharide derived from microorganisms include xanthan gum and gellan gum.

The skin indigenous bacteria balance improving agent according to the present invention, Staphylococcus epidermidis growth promoter, Staphylococcus aureus growth inhibitor, Acne bacteria growth inhibitor, glycerol production promoter by Staphylococcus epidermidis, antibacterial peptide production promoter by epidermal cells The solid content of the above-mentioned polysaccharide is preferably added so as to be contained in the range of 0.001 to 0.5 g per gram, and preferably in the range of 0.01 to 0.1 g per gram. More preferably, it is added so as to be included. In addition, in the case of cosmetics that are used daily, the solid content of the above-mentioned polysaccharide is preferably 1 in consideration of the fact that the total amount per use is usually about 0.5 g. It is preferably added so as to be contained in the range of 0.001 to 0.5 g per gram in a one-hundredth to one-fold amount, and 0.01 to 0.1 g per gram of 100 minutes. More preferably, it is added so as to be contained within the range of 1-fold amount to 1-fold amount.

(Forms of cosmetics and skin protectants)
The cosmetics and skin protectants according to the present invention may be in a suitable form for use such as ampoules, capsules, powders, granules, liquids, gels, bubbles, emulsions, sheets, mists and sprays. The cosmetics and skin protectants according to the present invention can be used as external preparations, and their form and classification are not limited as long as they are applied to the skin. For example, 1) topical drugs, 2) quasi-drugs, 3) cosmetics (for example, lotion, emulsion, cream, ointment, lotion, body rinse, facial rinse, oil, pack, mask, cosmetic oil) , Basic cosmetics such as cosmetic oil, facial cleanser, skin cleanser, scrubbing agent, massage agent, cleansing agent, makeup remover, degreasing agent, massage agent, depilatory agent, depilatory agent, shaving treatment agent , Aftershave lotion, preshave lotion, shaving cream, shaving lotion, suntan oil, suntan oil, suntan cream, suntan cream, suntan lotion, suntan lotion, whitening agent, body powder, talcum powder, bath powder, perfume powder, foundation , Powder, white powder, makeup base, makeup base, lipstick, lip balm, lip gloss, paste red, blusher, eye shadow, eyeliner, eye cream, eyebrow, eyelash cosmetics, mascara, eye makeup, eye makeup remover Makeup cosmetics such as, cologne, perfume cologne, eau de toilette, general perfume, perfume powder, perfume, perfume, fragrance, nail cream, nail enamel, nail enamel, nail enamel remover, nail enamel remover Agent, armpit odor inhibitor, body odor inhibitor, deodorant, deodorant, antiperspirant, deodorant agent, poultice agent, plaster agent, tape agent, sheet agent, patch agent, aerosol agent, etc.), 4) For scalp and hair Pharmaceutical and/or cosmetic preparations to be applied (for example, shampoo agent, rinse agent, hair treatment agent, pre-hair treatment agent, scalp treatment agent, press scalp treatment agent, permanent liquid, hair oil, polish oil, skim oil, Oil on bottles, set lotion, hair blow, hair brushing, tic, hair cream, pomade, hair spray, hair liquid, hair styling agent, hair color, hair dye, depigmenting agent, hair styling agent, hair restorer, hair nourishing agent, patch, Plaster agent, tape agent, sheet agent, patch agent, aerosol agent, etc.) 5) Bath agent to be used by pouring into bath water, 6) Others, hygiene products, sanitary cotton, wet tissue, disposable diapers, first aid bandages, cooling gel sheets Etc.

The cosmetics and skin protectants of the present invention can be applied to the skin by application, application, contact, etc., depending on the form. In the case of a bath agent, it can be applied to the skin by immersing the skin in bath water containing the bath agent.
Moreover, the application site of the cosmetics and skin protectant of the present invention on the skin may be local or systemic. In the case of local application, it may be any part of the whole body such as the head, face, neck, arms, abdomen, back, buttocks, legs and feet.
The number of times the cosmetics and skin protectant of the present invention are applied to the skin may be once or several times a day, or once every several days.

(Other components that can be used in cosmetics)
Such cosmetics can be produced by optionally selecting and using the components and additives exemplified below as long as they do not affect the effects of the present invention. The compounding amount thereof is not particularly specified, but it is generally considered to be preferable to be about 0.0001-50%.

(1) Oils and fats Avocado oil, almond oil, fennel oil, sesame oil, olive oil, orange oil, orange lafa oil, sesame oil, cacao butter, chamomile oil, carrot oil, cucumber oil, beef tallow fatty acid, kukui nut oil, safflower oil , Shea butter, liquid shea butter, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, mokuro, palm Oil, beef tallow, lard, squalene, squalane, pristane or hydrogenated products of these oils (hardened oil etc.).

(2) Waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, rice wax and the like.

(3) Mineral oil Liquid paraffin, petrolatum, paraffin, ozokeride, ceresin, microcrystalline wax, etc.

(4) Fatty acids Lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid, linolenic acid, docosahexaenoic acid, eicosapentaenoic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid Natural fatty acids such as, and synthetic fatty acids such as isononanoic acid, caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid, and isopentanoic acid.

(5) Alcohols ethanol, isopyranol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterols, natural alcohols such as phenoxyethanol, 2-hexyldecanol, isostearyl alcohol, synthetic alcohols such as 2-octyldodecanol ..

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol , 1,3-butylene glycol, pentyl glycol, glycerin, pentaerythritol, threitol, arabitol, xylitol, ribitol, galactitol, sorbitol, mannitol, lactitol, maltitol and the like.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyldecyl dimethyloctanoate, cetyl lactate, myristyl lactate, Diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, etc.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) Gum, sugar or water-soluble polymer compound gum arabic, benzoin gum, dammal gum, guaiac fat, Irish moss, karaya gum, tragacanth gum, carob gum, quince seed, agar, casein, lactose, fructose, sucrose or its ester, Trehalose or its derivative, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2-C4) chitin or chitosan with added alkylene (C2-C4) oxide such as ethylene oxide, low molecular weight chitin Or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or its salt, hyaluronic acid or its salt, chondroitin sulfate or its salt, heparin, ethylcellulose, methylcellulose, carboxymethylcellulose, carboxyethylcellulose, carboxyethylcellulose sodium , Hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylates, polyalkylene oxides such as polyethylene oxide and polypropylene oxide, or crosslinked polymers thereof, carboxy. Vinyl polymer, polyethyleneimine, etc.

(10) Surfactants Anionic surfactants (alkyl carboxylates, alkyl sulfonates, alkyl sulfate ester salts, alkyl phosphate ester salts), cationic surfactants (alkyl amine salts, alkyl quaternary ammonium salts), amphoteric Surfactant: Carboxylic acid type amphoteric surfactant (amino type, betaine type), Sulfate ester type amphoteric surfactant, Sulfonic acid type amphoteric surfactant, Phosphate ester type amphoteric surfactant, Nonionic surfactant ( Ether type nonionic surfactant, ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing nonionic surfactant), other surfactants ( Natural surfactants, protein hydrolysates derivatives, polymer surfactants, surfactants containing titanium/silicon, fluorocarbon surfactants), etc.

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), Riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acid, pantothenic acid, biotin, choline, inositol, vitamin C group: vitamin C acid or its derivative, vitamin D group: Ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotaxosterol, vitamin E group: vitamin E or its derivatives, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K2), menadione. (Vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (rutin, eriocitrin, hesperidin), vitamin U and the like.

(12) Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine , Arginine, ornithine, histidine, etc., and their sulfates, phosphates, nitrates, citrates, or amino acid derivatives such as pyrrolidonecarboxylic acid.

(13) Various additives derived from plant- or animal-based raw materials These are the types of products to be added, and the processing (for example, crushing, milling, washing, hydrolysis, fermentation, purification, which is performed in a conventional manner according to the form. , Squeezing, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc. are arbitrarily selected and combined, and various materials are arbitrarily selected. You can use it.

It should be noted that the solvent used for extraction may be selected in consideration of the purpose of use, type of product to be provided, or processing to be performed later, but usually, water, methanol, ethanol, propyl alcohol, isopropyl alcohol, butanol. , One type selected from lower alcohols such as isobutanol or hydrous lower alcohols, polyhydric alcohols such as propylene glycol, 1,3-butylene glycol and glycerin or hydrous polyhydric alcohols, and various organic solvents such as acetone and ethyl acetate Alternatively, it is desirable to use a mixed solution of two or more kinds. However, when the content of the organic solvent is not preferable depending on the application, only water may be used, or ethanol that is easy to remove after extraction may be used and used alone or in any mixture with water, or, Exploited extract may be used.

In addition, when the additive derived from plant or animal raw materials is applied to the external preparation for the whole body or topical use, cosmetics, including protection of skin and hair, moisturizing, improvement of touch and texture, imparting of flexibility, stimulation Relaxation, stress reduction due to aroma, cell activation (prevention of cell aging), suppression of inflammation, improvement of skin and hair quality, prevention of rough skin and its improvement, hair growth, hair growth, hair loss prevention, luster imparting, cleansing effect, In addition to cosmetic effects such as relief of fatigue, promotion of blood flow, and warm bath effect, effects such as scenting, deodorization, thickening, antiseptic and buffering can be expected.

In addition to the above, various cosmetic and pharmaceutical effects of each raw material known so far are expected, and by combining these, the effect aimed at by the present invention can be enhanced, and It is also possible to make the product expecting a functional effect.

Specific plants as raw materials (herbal medicine) include, for example, almonds (hen peach), eyes (indigo), blue vine (seifuto), aoki (aoki), aogiri or kenashiaogiri (gokin), Aozuzurafuji (Kibomi) , Aotenma, Acacia, Red scabbard (red hemp), Redcurrant ``fruit'', Red clover, Red grapes, Redwood (Logwood), Redheaded wrinkles (Red bud), Redhead madder or Akane (Akane grass root), Red radish or Diou (ground yellow), Agi (A Wei), Aki larch, Akinare (Reimu Hide), Aguay Gua Su, Aguahe (Omi Tenguyashi), Akebei (Kizu), Asa (Asako Jin), Malba Morning Glory or Morning Glory (chocolate), Hydrangea (Hydrangea), Ashitaba (tomorrow leaf), adzuki bean (red azuki bean), asunaro, acerola, ashen yak (asenyaku), athira (shokuyoukanna), anise, abiu, abiurana, canola, abemaki "fruit", avocado, flax, amacha (sweet tea), Amatachuru, Amadokoro (Tamatake), Amana (Kotobuki), Amaranthus (Hiyu, Hagatetou, Himogeito, Senninkoku, Sugimorigatetou, Hosoaogateou, Aogatetou, Haribiyu, Aozulan, Amaranthus hippoconduricus, Agaranthus hippocondariacus), Amigasu Aristromelia (Yurizuisen), Arcana (Arcanna), Argarobo (Cabbage), Altea, Arnica, Alpinia or Katsumadai (Souzuku), Aloe (Lord), Aloe Vera, Angelica, Apricot or Hon Apricot (Apricot kernel), Ansokkou (Saint Aroma), Yellow Saute , Igakouzorina (geiliary head), Epimedium or Dioscorea japonicum (Indian cabbage), Igusa (Wicked grass), Knotweed (Traffic root), Yew (first place), Strawberry, Fig (figures "fruit, leaves"), Fig First), ichibi (winter aoi), chinensis (one medicinal herb), ginkgo (ginger ginkgo ``seed, leaf''), Itohimehagi (distant), locust bean, Aedes chinensis (Teilexi), pearl millet (Honagainubiyu), rice ``seed, seed coat'', Inondo ``seed'', sardine moss, nettle, ylang-ylang, iwatobacco (Iwabaki), iwahiba or iwamatsu (cow roll), common bean, oolong tea, fennel (boiled incense), wheat (chibana), ukiyagara (sanriku), kusu "Fruits", Scallops, turmeric, duckweed, Usubasaicin or Keirinsaishin or Oushi Chrysanthemum (spicy), pearl oyster, pearl oyster, hollyhock, orchid, hemlock (summer hay), oyster or cypress (anchored, activated, activated), unyadegat (uncarria, cat's claw), uba tea, ume (wheat seeds, pulp) )), Vladimir oak, Japanese oak (Lepidoptera), Unshu mandarin (Chen skin), Unboku, Estragon, Ezokogi (Ezo Goka), Ezo Suzuran, Echinashi (Pleurotus chinensis) or Enishida, Enokitake (Akaebisu Akira), Enoki (Agaricus vulgaris), Enoki ), Elcampre, Elderberry "fruit", Elemi, Eryngii or Pre-lotus erynge, Enju (Sophora, Sophora rice), Pea, Scutellaria or Kibana-ohugi (Yellow radish), Narukoyuri or Kagui Kurumabanarukoyuri (Yellow essence), Sasayukiyuki Or Yuki Sasano, Oren (Yellow), Ogatta Houken, Okarasuri (Calocon), Ooguruma (Civil incense), Otsutsurafuji (Self-defense), Psyllium (Carpin, Carp grass), White thistle (Yoshu Leopard, Bald Oak), Otaku (Haruko), Toucan Cactus, Obana okera or Okera (Shiroi), Obana crape myrtle (banaba), Obayashishabushi "fruit", Coccinellus chinensis (German herb), Oomiacatetsu, Omihawthorn or Hawthorn (Orange palm), Oomimi ), oka, okazeri (snake bed), okra ``fruit'', oguruma (overturning), okururihigotai (bamboo leak, indigo head), ginseng or botanical ginseng (carrot), hypericum or sycamore, or hypericum perforatum. Younger brother's cut grass, Odorikosou (interrupted), Onami fir (blue ear), Onigurumi, Onidokoro or Tokoro or Nagadokoro (Haikai), Oni no Yagara (Tianma), Oniyuri or Sasayayuri or Hakatayuri (lily), Onoeran, Ononis, Ohahyou ), Ominaeshi (soy sauce), Oyuco, Dutch mustard (watercress), Dutch jelly, Dutch bieu, Dutch honeywort, olive "fruit, seed, leaf", oregano, orange "fruit, fruit skin", cauliflower (ripe yellow), cacao "Fruits, peels, seeds", oysters (persimmon "leaves"), oysters (fishing hook), oysters or kantrisou (rensenzo), oysters, hydrangea, cassia, casino oysters (fruit "fruits"), gadgets (Ryoke), Kashiwa (camellia tree, camellia leaf), Cascarilla, Cascara Grada, Kasumiso, Kasenso, Crabworm (Kinshato), Kaniwa, Ka Nation, valerian root, birch, birch or weeping birch (birch), gerbera, pumpkin, kapok cypress ``seed'', kahoksansho (pepper), gama (yellow yellow), chamomile or roman chamomile, chamomile (camgrass), camouflage (Duck), color, larva, mandarin or crow cucumber (sea melon), crow bischak (half-summer), oats, guarana ``seed'', callaho (basket), karaya, garyu, karin (wood melon), garcinia, kawamidori, kawarasaiko (Koryo greens, white grass), kawajisha, kawaratake, kawaranadeshiko (ishitake), or ezkawaranadeshiko (red wheat, dry wheat), kawaran carrot (green glutinous), kawara mugwort (chinen chinse), kansui (sweetened), kanzo (kanzo). Licorice), cantara, candelilla, canto, canna, kanran, raspberry (ezo strawberry, dutch strawberry, shrimp strawberry, nawairo strawberry, maple strawberry, european raspberry), kiwi ``fruit, leaves'', cichlid cucumber (melon root), kyokyo (kikyo, bellflower) Roots), chrysanthemums (Chrysanthemums, zebra daisies, Astragalus membranaceae), hollyhock, cattle (Azusa berries), Rumex (Yotei root), Phellinus linteus (Kimitsu), Kizuta, Kidachialoe, Kachihachika, Kina, Kinakinoki (Shinkona, Akakinakinoa), Kinua (Kinua) ), Kihada (yellow oak), Kimame, Gymnema sylvestre, Kimenkak, cabbage, cabbage "unripe fruit", caraway, cucumber, goryu (Nishikawayanagi, Teirayanagi), Kiraja saponaria, Kiraya, Kiransu (Kenshi small grass), Kumquat “fruits”, quince, ginsekai, kinbusen, betel, quince, quince, ginran, quinran, guinea, guapeba vermelha, guava “fruits”, guayule, quercus infectria (galaxy), kukui nut, kugenumaran, kuko杸杞, 杸杞子, 杸杞leaf, skeleton), sedge vine (Tenmon winter), kudzu (Kakkon), camphor tree, gooseberry "fruit", quinces (yellow flowers), kutiti river, gardenia (Yamashiko), kunugi (Kunugi) (Kou-Sou), Kupuasu, Kubeba, Kumazasa, Bear vine (Clubweed), Clara (Gourd), Cranberry "fruit", chestnut "seed, fruit, astringent skin", Curculigo latifolia "fruit", grapefruit "fruit/leaf", Black buckthorn, black squirrel (necessity of rescue), Kamehiba hikoshi or Kurobana Hiki koshi or Hiki koshi (Enmei grass), clove (clove, clove) , Gumbai Nazuna (Sekimei, Sekimeishi), Keigai (Purpleum serrata, Paepa spp.), Keitou (Corolla flower, Corolla), Geckavidin, Bay (Laurel), Kenshi Sartoriibara (Peonyphorus japonicus, Mountain return), Gentiana, Gencho-koso , Kemponashi (Kigushi), Koukisekekoku, Kishikankan (Koji, Tachibana, Obenimikan, Fukuromican, Sagamikouji, Ponkan, Santara (Tachibana peel)), Koshinbara (Monthly flower), Kousuihakka, Kozo (black tea), Kocha (black tea) , Kouhone (river bone), Kouhone (straw book, Karamoto book), Kouryan, Kouryokyo (Kora ginger), Cilantro "fruit", Coleoptera (Huang Huang Ren), Scutellaria (yellow gon), Cowberry (overgrowth), coconut "fruit" , Goshuyu (Gourd mushroom), Gosho strawberry (Okubonko), Pepper (Pepper), Cosmos, Copaiba balsam, Coffee "seed, leaf", Kobushi or Magnolia (Spicy), Burdock (burdock, beef chopsticks), Koboduzu, Sesame (sesame) ), sesame seedless (genus), trash (schisandra), rapeseed or binankazura or pine beetle, wheat (wheat), rice or rice bran ``red bran, white bran'', rice oil, cola acminata ``seed'', cola vera ``seed'' , Colonia, fenugreek "fruit", Colombo, Conzurango, kelp, konjac, comfrey (final grass), sisal (sisal asa), saihairan, sakaneran (ezosakaneran), saxima button vine or sycamore vine or sinenensis (seireisen), sakura (Oshima cherry, Yamazakura, Oyama cherry, Ed Higashi, Mamezakura, Miyamazakura, Someiyoshino, Takanezakura, Kasumizakura, Kohigan, Satozakura, Kanzakura "leaves, flowers, fruits, bark (cherry bark)", cherry, pomegranate, sasasa, sasanbagin , Sageomodaka (Sawaki), sweet potato, sugar cane, sugar beet, Sanebutojutsu (Acme Natsume), saffron (bancoflower, west safflower), sapodilla, sapote amariyo, pomelo "fruit", sabonsou, salmon berry "fruit", salinach ), salancito (sand indigo stab), salvia (sage), scallion (mountain spinach), sawawami (mountain walnut), Sancactus, Sangre Degrad (croton), Sankusou, Sanshichin ginseng (37 ginseng), Sanshuyu (Yamatake mushrooms), Sansho (Yamasho), Sandscon (Yamame root), Shea (Karite), Cyanoki "fruit", Shiitake mushrooms (Shiitake mushrooms), Shi On (Shien), shikakumame, digitalis, shikunushi (shikuniko), perilla or Aojiso or Chimenjizo or Catamendiso (Shiso leaf, Shisozu), rosewood, linden, Chinese holly, Chinese forsythia (couples), shimeji mushroom White-tailed shimeji, beech-shimeji, honjimeshi, swordweed), magnolia (spicy), spirea, potatoes, shajin (shasan), jasmine (matsurika), janohigashi (wheat gate winter), juniper magnolia, sand syrup, sandworm Jin, curd sand), juzudama, palm "fruit", shunran, swordweed, ginger (ginger), hydrangea hydrangea (tsuneyama), ginger (iris, iris root), shozuku "fruit", white oak "seed", silane (butterfly) ), white croaker ``seed'', white clover (white clover), white adzuki bean (chicken bone grass), white squirrel (smell iris), white natsuta ``flower'', white lupine, shirominanten (southern nut), cinchona salmon sylbula, ginchoge (mizuka, mizuka) (Flowers, shanging flowers), cinnamon, cinnamomum cassia (cinnamon), watermelon (watermelon), honeysuckle (gold and silver flowers, Shinobi winter), sorrel (acidic), swimberry "fruits", star apple, stevia, strawberry "fruits", sycamore ( Surucho), horsetail (interrogation), purslane (horse toothpaste, horse toothbrush), plum "fruit", sulgalan (oran), red pine "cone", larch, black locust, black walnut, hawthorn, Stuffed dandelion, Horse chestnut (horse chestnut), Pear "fruit", Salix napus, Elderflower (Elder), Negate (Juniper, Juniper), Achillea millefolium (Milfoil), Rose sardine, Steller's mistletoe, Steller's mistletoe or western Yamahakka, horseradish, Sekishou (Sekisho root), Dendrobium (Sakura Sekkoku, Koki Sekkoku, Oba Na Sekkoku, Okinawa Sekkoku, Honsekkoku, flatulence Sekkoku, square Sekkoku, Kiba nano Dendrobium) (Dendrobium, Dendrobium), Sedoron (Boushuuboku), Mallow, Broadbill Senega, Senega, Seri, Celery, Senkyu (River Kyu), Senshinren (Shinshinren), Sendan, Sennin Cactus, Senna "fruit, leaf", Senninsou (Sweet), Senburi (medicated), Senboku, souka (Grass and fruits) , Elephants, soybeans, buckwheat "seed", some potatoes, broad beans, rhubarb, daikon, daikon, soybeans, soybean, daidai (orange skin, drought), takasago rurihigotai (south indigo spine), takasaburo (drought lotus), takatodai ( Ootsu), Takawarabi (in Japanese), dark sweet cherry ``fruit'', hollyhock, pearl oyster (thyme, lily of the valley), taikodokoro (hikai), willow willow, tamarind ``seed'', onion, tamshiba (spicy), taranoki ``fruit,'' Leaves, root bark", tarayo (Oba winter blue, one leaf tea, bitcho tea, bitter tea),
Dahlia (Tenjikubotan), Taruwi, Tankimimame, Tanchiuchiwa, Tanjin (Tanjin), Dandelion (Kankei English) or Shirobana Dandelion or Moco Dandelion, Danmal, Cherry "fruit", Chigaya "fruit, root, bud", Chixeteng carrot ), chicory, chanka piedra (yellow citrus), tulip, choconostay, artichoke (artichoke), ginseng (daihuang), ginseng (garden), ginseng (garden leaves), choreimaitake (boiled boar), Chorogi, Tsukimiso, Tsukushimakaneran, Tsukuritake (Mushroom), Tsuchiakebi, Camellia, Centella asiatica, Clover (lacquer grass), Tsuyakusa (Duck rush), Tsuzuki Azuki (red azuki bean), Tsurutsukichia, Tsurukudami (Apricot), Tsukudami (Apricot vine), Tsutsukudami (Apricot) Ginseng (Folia ginseng), Glycine soja, Tsuwabuki, Deiko, Ganoderma lucidum, Duberry "Fruit", Agar-tencha, Tencha (Japanese tea), Tendaiyaku (crow), Pepper (winter melon), Pleurotus cornucopiae, Pepper (Pepper), Pepper (this) Return), quince mosquito (marigold), sugar beetle, cornflower (river cut), corn or corn hair (nanban hair), corn mouse moth (maiden tree), gentian or gentian (dragon gall), horse chestnut (tree bandit), dokudami (dokudami) Ten medicines), konjac (root root), horsetail or bean bug or narcissus, Eucommia vulgaris (Musaka “bark, leaves, roots”), ash (harp), tomatoes, tragacanth, trianthanthus “seed”, lisianthus, tormentilla, droneki, troloaoi , Tonkamame, Nigeria berry "fruit", Chinese yam or yam (mountain drug), Nagikadada (Butcher bloom), Naginakokouju, Nazuna, Rapeseed, Beans or Tatehaki (Japanese sword bean), Natsumikan, Jujube (Omugi), Naniwaibara (Gold) Sakurako), navena (intermittent), nameko, peanut (peanut), nanten (nantenmi), nanbankaramushi (ramie), nioisumire, nigaki (bitterwood), bitter wormwood (buffalo), nutmeg, kei or nikkei or ceylon. Nikkei or Yabunikei (cinnamon bark) or Keishi (Keishi), Nyoro pudding, Chinese chive (Nishi), elderberry (bone grafted tree "fruit, flower, stem, leaf"), garlic (daikon), nurude (quintile), leek, alder or Nebu or Nebi or Nemurinoki or Jigokubana (Nemours), Thistle (Oyagi), Neubara (Yuyomi), Nozomi Kazura (Ryohika), Nogetou, Yarrow , Nodake (pre-hu), Novara, Peach, pineapple "fruit", Hibiscus (Bussouge, Furuin Bussouge, Roselle), Hyringou, Hauchiwa, Pau-Dose, Hakamauraboshi (Bone-supplementing), Hakukayumato, Hakusantori, Hakusen Skin), huckleberry ``fruit'', chickweed (harsh), hazel (harbor), hasdikoro (roth root), basil, lotus (lotus, lotus meat, lotus root), parsley (holland jelly), hadakamugi, batata, hachiku or medake (mushroom). Bamboo boiled), patchouli, peppermint (thin leaf, thin leaf), Mucuna pruriens, pearl barley (Yokuinin), Hanasuge (Chinese mother), banana, Hanahakka, Hanabishi (Shishishuri, Shishitsuri), vanillabins, papaya, Hahakogusa (Musclegrass), Pahalobobo , Hub "whole grass, stems, leaves", paprika, Hamakiran, Hamago or Mitsubahamago (vine vine), Hamasuge (Kafuzi), Hamabishi (Shituriko), Hamanasu (maikai flower), Hamaboufu (hama windbreak), Hamamelis, Palm, rose, balata, balata, agaric, agaric, agaricus, ukimorinokasa, harlan, paliurus, palletaria, halkanlan, balsamina (vine, nigiri), harunire (Yuha), Barbasco, Harlindou, Handaikai (Hatsudaikai), Breadfruit, Hyogi (Hikari), Leek Ginseng (Ginseng), Pecan nuts, Higanbana (Gypsum, Ginseng Shaka), Higulmadaria, Higotai, Hishi (Hishi) Fruit), pistachio, beet, tsutsumi (Ishidashi), hibiscus edulis, daisies (daisies), Hinako no Kochi (cow knees), Hydrangea, Hinoki, Hiba, castor, sunflower, peppers, himezu (maggot), Himegama (incense) , Himematsutake (Kawariharatake, Hiromatsutake), Himeyouran, Pimenta "fruit", juniper, junior beak, kyuyu "fruit", chickpeas, lentil, wildflower mallow, white pearl oyster (Bald-headed), loquat "fruit, leaf, stem" Large clothes, 榳榔子), Pu'er tea (Fu 洱 tea), Futokazura (nantan), butterbur, butterbur dandelion (subsistence winter flower, subsistence winter leaf), wisteria mackerel (orchid grass), wisteria beans (flat bean), wisteria wisteria (Flower flower), Grape ``fruit, peel, seed, leaf'', beech, Fuyusuminashitaketake (caterpillar), Brazilian licorice, Brazilian carrot, Brazilian palm, Brazilian elephant palm, Black carla "Fruit", blackberry, blanchen, plum "fruit", fulceraria, blueberry (Himesuhime), prune, floral blanka, blond psyllium, bundou (mung bean), hazelnut, loofah, hetkaran, safflower (red flower), Henneken, Belladonna, berry ``fruit'', Persea, Perbianberg, Pereschia grandifolia, Benchisou or duckweed (Keiten), Boysenberry ``fruit'', broomwood or sageweed or nemou or Hahakigi kokia (ground silk), hosairan, housenka ( Fengxian, acute child, skeleton, Pouteria sapota, Pouteria lucuma, honoki, bow-fu (windbreak), spinach, physalis (Torone), honoki (Japanese pomegranate, magnolia), bokeh (wood melon), hosoba abragiku (bittersweet) (Yoku), Hosobaoguruma, Hosobanaokera (Soshu), Hosobanokirinso (Kagetensanchi), Hosobabarengiku, Bodaiju (Bodhi tree), button (peony "flower, leaf, stem, bark"), button pi (peony skin), hop , Jojoba, Boriji (Ruricha), Bordeaux, White berry "Fruit", Hongonik (Meat Juyo, Daiei), Hongsekoku (Tempered Peeling Stone, Suzuyama Ishiharu), Maizuru Tennansho (Tennansei), Maitake (Maitake) ), maoh (mahuang), maca, macadamia nuts, margaret (mokushungiku), macri (seagrass), magwa (mulberry bark "bark, leaf"), magnolia sprengueri, masalandouba, masala nuba de ceara, maceira De Boi, Mashua (Tamanouzenhallen, Kyukonkinrenka), Matatabi (Kiten Tate), Matsukasa, pine "leaves, bark, roots", Matta Orho, Matsuhodo (芓苓), Matiko (Kordonshijo), Mayoram ( (Hana mint), Malvano jazin (sardine), Mulberry "fruit", quince, malein (biloud moth squid), mango, mangosteen, manzania (Andes chamomile), manshu gurumi, mandarin "fruit", manensis (reishi), pheasant (concrete) ``Fruit''), Mishima Psycho (Saiko), Water plantain or Water morning glory (Rhinoglossum), Water beetle (Hanbian lotus), Misohagi (Cendula vegetable), Michiyanagi or Niwayanagi (Henho), Mitsugashiwa, Mitsuba, Midorihapota, Midorihakka, Mimosa, Myoga, Miracle Fruit "Fruits", Myrrh, Myrobalan, Strawberry, Mukuge (Kaki), Mukunoki, Makuriji (extended skin), Munya, Murasaki (purple root) ), Murasakikib or Oomurasakikib (purple pearl), Murasaki corn, Murasaki Natsuji (Kunmei chicken blood wisteria), Megakansa opuntica, Mehajiki (improved mother grass), Mebougi, Melaleuca, Melissa, Meliloth, Melon ``fruit'', Mokomyogi, Mosouchi, Mokugirin, Moje (Pepper Box), Ilex, Mokkou (Woody incense), Pleurotus communis, Peach (Peach "leaf, seed, flower, fruit"), Moyashi, Morero cherry "Fruit", Moroheiya (burlap), Yawakawamurasaki Potato, yakchi (maschi), cornflower (cornflower), cornflower, yacon, yashabushi (arrow wheel) or Himeyashabushi or Oyabayashabushi ``fruit, fruit skin, fruit ear'', willow willow, yamde (octagonal metal plate), mistletoe (willow parasitic), Willow (Kiyanagi, Tachiyanagi, weeping willow, red willow, pussy willow, dog cory willow, willow willow, cory willow, unrye willow, palm, willow willow, willow, willow, willow, willow, willow, willow, willow Beef), Yabu Tobacco (Tsuru and Tenmei), Yamabobo (Shokuriku), Yamanoki (Yamaharu), Yamamomo (Yangmei), Yamamogomi, Eucalyptus, Yukinoshita (Tiger Ear Grass), Yucca or Flavifolia, Yuzu "Fruit" , Lily, yellow sedge, mugwort (barley leaf), ligan (thunder circle), lime "fruit", rye, lakanka "fruit", raspberry "leaves, fruit", latania (clamellia), rakkyo or shallot (caucasian), lavender, longan (Long-eye meat), agave (Ano agave, fukurin agave), ryokcha (green tea), apple ``fruit, seed, leaves, roots'', gentian, rubus or Suabisimus (white cool), Ruritama thistle (urajirohigotai, shinjia stab head) , Litchi (lichi, lichi kernel), lettuce (chisha), red currant "fruit", red pitaya, lemon "fruit", lemongrass, forsythia or Chinese forsythia (renzoku), astragalus, waxy wax, wax palm, locon (rocon) Reeds, roots, logan berry "fruits", rosemary (mannen wax), rose hips (novara), wasabi, cottonweed (wooden flowers), waremokou (earthenworm) and the like.

Examples of seaweeds include seaweed [green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Aonori (Usubaaonori, Sujiaonori, Hiraaoonori, Bouaonori, Hosoedaonoori), Anaaosa (Aosa): Seaweed (Macombus, Risikomu, Hosomekonbu, Mitsuishikonbu), Wakame, Hirome, Aowakame, Giant Kelp (Macrocystis pilifera, Macrocystis integrifolia, Neocitys ruetokeana), Hijiki, Hibamata, Umiuchiwa, Usubaumi Nobiwa, Kibiwaki Black sea urchin, Red sea urchin, Red sea urchin, Red sea urchin, Red seaweed, Seaweed, Red seaweed [Red algae: Hijirimen, Maxa (Agarulaceae), Hirakusa, Oyakusa rindaku, Noriha sugi, Noctuida, Yatarigusa, Oyakuduki, Yakutaku, Yakutaku, Yakutaku, Yakutaku, Yakutachi, Giraffe, juniper giraffe, tsunomata, obatsunomata, tochaka (yahazunonomata), ezotsunomata, tetsunonomata, hirakotoji, kototsunonomata, hibotsunorimori, marubatsunomata, hirakotoji, suginori, shikininori, hirakotoji It is mentioned as a thing.

In addition, other algae, for example, green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Cubanomim: Quanonimo, Volvox: Ohiogewawari, Volbox, Palmera, Yotsumeo, Aomiduro: Hisaori, Aomiduro, Tsurugimidoro, Hibimidoro: Hibimidoro, Aosa: Anaaosa, Amiosa, Nagaaosa, Kawanori, Kawanori, Furitchera, Shiogusa: Oshiogusa, Asamidomata, Marimo, Baragonia, Marimo, Baragonia Genus Magata: genus Magata, genus Iwatari: genus Ibis, staghorn ivy, swelling ivy, black ivy, genus genus, genus mil: mill, cromil, sakibuchimir, nagamill, hiramir, genus Kasamori: nocturnal moss, juvenile moss, juvenile moss, scorpion, lizard, lizard, lizard Genus, genus Tsudumimo, genus Amblyopia: genus Amblyopus, genus Amblyopsis: human Exa, genus Heloha Notohito, Usuhito Exa, Motchihitoe, Sayamidoro, genus Kunsumomo, Violet genus: Violet vulgaris, Genus Fushimamidoshi, etc.).

Cyanobacteria (Suisenzinori genus: Suizenzinori, Aoko genus, Nostoc genus: Kawatake, Nostoc commune, Hassai, Yuremo genus, Lasenmo (spirulina) genus: Spirulina, Trichodesmium (Aiacacio), etc.).

Brown algae (Pyraera: Pyraera, Shiomidoro: Nagamisio Midoro, Isogrape: Isogrape, Isogawara: Isogawara, Black-faced squirrel: Gunsen Black-headed Gill, Kashirazaki: Kashirazaki, Muchimo: Muchimo, Hiramushimo, Prunus chinensis, Amphitidae: Aphidida , Genus Thysanoptera: Ceratophyllum, Fukulinamiji, Common genus: Common gussa, Yahagusa genus: Ezoyahaz, Yahagusa, Uraboshiyahazu, Jamigusa: Jigumigusa, Cyperaceae genus: Cyperus cypresses, Cyperus cypresses, Cyperus cypresses : Nabarimo, Chasomen: Motchichasomen, Matsumoto: Matsumo, Nagamatsumo: Nagamatsumo, Okinawa mozuku: Okinawa mozuku, Nicemozuku genus: Narcotic mozuku, Physcoma genus: Physcoma: Genus moss Black-necked moth, mozuku genus: mozuku, ishige genus: ishige, iroro, ichimegasa genus: ichimegasa, keiya genus: keiya, sea urchin genus: sea urchin, urushigusa: urushigusa, kyurushigusa, tobacco butterflies: mosquito genus: moss moss : Habanori, Habanori Genus: Habanori, Common Bukuro Genus: Common Bukuro, Ezobukuro Genus: Ezobukuro, Fukuronori: Fukuronori, Cottonweed, Chishimafukuronori: Chishimafukuronori, Kagomenori: Kagomenori, Muraridusa, Murakudakusa, Murakudakusa, Musashikudusa Genus: sardine, leafhopper, genus: leafworm, genus: porridge, genus: fennel, genus: genus: tumo, genus: genus: genus: genus: genus: genus: genus: genus: konjac, konjac, konjac, konjac, konbukon, konbukonbu, konbukonbutsu, konbukonbutsu, konbukonbutsu, konbukonbutsu, konbukonbutsu, konbukonbutsu, konbutsubutsubutsu, konbukonbutsu, konbutsubutsubutsu, konbutsubutsubutsu, konbutsubutsubutsu, konbutsubutsubutsu, konbutsubukonbutsu, konbutsubutsubutsubutsu, konbutsubutsubutsubutsu, konbutsubutsubutsubutsukon Green kelp, Gohei kelp, Naga kelp, Pea kelp, Great blue kelp, Trolocomb genus: Trolocomb, Antocume genus: Antokume, Kazume genus: Kajime, Tsuruarame, Kurome, Chrysanthemum genus: Chrysanthemum genus: Black kelp, Black locust, Black locust, Black squid genus Catfish, Ala genus: Alame, Ainu Wakame: Ainu Wakame, Chigaiso, Oniwaka Ezo Ishige: Ezo Ishige, Yabanemoku: Yabanemoku, Rappamok: Rappamok, Jolomok: Uganomomoku, Jo Lomoku, Hiemoku, Hondawara genus: Tamanashimoku, Isomok, Nagashimamoku, Akamoku, Shidamoku, Hondawara, Nejimoku, Nara Samo, Mametawala, Tatsukuri, Yamokumokaku, Miyokunoha, Ohabokuku, Fushizukumokoku, Hagoku-hakoku, Haburakumoku, Fushizukumokoku, Hagoku-hamo, Omomoku. , Genus Dermatophyta: oleander, genus vulture: genus vulture, genus Kayamonori: Kayamonori, etc.).

Red algae (Genus: Ushikenori, Funorinoushiuke, Amanori: Asakusanori, Susabinori, Upruinori, Oniamanori, Tasa, Fuiritasa, Benitasa, Rhodocorton: Mizobenako, Konohadako-no-hadokona, Hibakuna-kodako, Kobo-no-kodako, Kobo-no-kodako Genus: sea urchin, black squirrel, duck moth, mosquito genus: benimozuku, hosobeni mozuku, pine genus: pine spruce, fusanori genus: fusanori genus: genus genus dendrachium cylindrica, mosquito mosquito moss, mosquito mosquito lactam Genus: Tamaitadaki, Kagenokiri: Kaginori, Kaguyenori, Agar-genus: Amanita, Hitentha, Oobusa, Nambugusa, Kohira, Yohira, Quincus, genus: Species of the genus: Species: Himuradyu, Rhizophora, Rhizophora, Rhizophora, Rhinoceros , Heralyumon, Narcissus communis: Narcissus communis, Okitsubara genus: Psyllium cypress, Aqaba genus: Aqaba, Marva aqaba, Naminohana: Hosobana minohana, Naminohana, Anemoneaceae: Genus Nephila, Anemoneaceae: Genus Anemone , Kaninote: Kaninote, Coralberry: Coralberry, Corydalis, Centipede: Centipede, Sugimurade, Katanori, Hiramukade, Kyonohimo, Sakura nori, Nikumukade, Tambanori, Tsurutsuru, Nerutakutaku, Fukuroku, Nukurakuo, Nakurakuo, Nukurakusa, Nukurakusa, Nukurakusa, Nukurakuo, Nukurakuo, Nakurakuo, Nukurakusa , Genus Matabo, genus Polyphyllum, quintaceae: charlotte, quintet, pine tree, rice bran, mackerel, pine tree, pine tree, fushikintoki, tsunomukade, nagakintoki, suzumukade, kakureito: plantain, mosquito genus , Fukurofunori, Mahunori, Pleurotus: Pleurotus, Pleurotus: Pleurotus: Pleurotus, Pleurotus, Pleurotus, Pleurotus, Pleurotus, Pleurotus, Pleurotus, Pygnus Pleurotus, Quercus genus: Quercus vulgaris, Ezotosaka genus: Ezotosaka, Tsukasanori genus: Enashi Calimenia, Otsukasanori, Hana Gata Calimenia, Honoo: Honoo, Hikkagenoito: Hikagenoito, Usuginu, Nikuhouno: Nikuhouno, Venezugo genus: Venice Nago, Suskakebeni: Suscaquebeni, Okamuragusa: Yamadagusa, Mirin, Pleurotus scutellaria, Tosokarina, Tosaminami, Tosamomirin, Tosaminamirin , Isomocca: Isomocca, Yukari: Yukari, Hosoyukari, Ibaranori: Ibaranori, Saidaibara, Tachiibara, Kagiwaranori, Physcomitrella: Physalis, Isodanthus: Isodantsuu, Atsubanori: Atsushi Nori, Ogonori, Ogonori, Ogonori, Ogonori Mizookonori, Kabanori, Beniogonori, Fucresinori, Purple Cabbage, Shinkai Cabbage, Togekabanori, Catagogo Nori, Ryukyugonogori, Seigogonori, Ittsugi-nosugi-no-kyunori, Mitenaganogori, Misakinagaonogori, Misakiogonorigori, Misakiogonorigori Genus: Fushiklenori, Namibia genus: Narcissus lanceolata, Strains of the genus: Spheniscus, Cyperaceae: Okitsunori, Sacred genus: Evening grass, Saimi, Harigae, Lotus japonicus: Lotus japonicus, Anemone japonicus, Anemone japonicus, Anemone vulgaris, Anemone , Genus Astragalus: genus Astragalus, genus Rhizophora: Hishibukuro, Madaragusa genus: Togemadara, Etsukimadara, Tao Goatou: Tao Goatou, Hanasakura, Scrophular genus: Scutellaria, Goatweed genus: Rhinogobius genus: Lagoonidae: Hananoeda: Hananoeda , Scorpionfish, Anadalus, Webagus genus: Weebagsa, Benifuronori: Benifuronori, Fusutsuna: Fusutsunagi, Himefusutsugi, Hirohafushitsuna, Ventus vulgaris: Dendrobium vulgaris, Habitaria, Haginotus, Hydrangea, Hessian, Anthony Genus: Egonori, Futogigis, Saeda: Saeda, Chirimomiji: Chirimomiji, Konohori Family: Habatae Nori, Konohanori, Suzuronori, Usubini: Usubini, Hasujiinu: Hasujiginusu, Nagasujinaginu, Nasagijinogien, Nagakojinohari, Naganojinoha Nori Kagious Banori, Yareus Banori, Suzius Banori, Haius Banori, Usui Norimodoki: Usbanori modoki, Ayanishiki: Ayani Shiki, genus Ayaganu: genus Ayaganu, genus Dahia: genus enashidasia, genus Shimadsia: genus Isohagi, shimadasia, dajimodoki: daidomodoki, itogusa: moloitogusa, futoigusa, makuri genus: makuri, willowagirinagi, swordfish, weeping dragonfly, dragonfly, saccharomyces , Sasabayanaginori, Sozo: Kurosozo, Kobusoso, Hanesoso, Sozonohana, Hanegusa: Hanegusa, Haemongus, Cosanemo: Cosanemo, Isomurasaki, Hosocozanemosugi, Oshigosugi, Higurashi, Higurashi, Higurashi, Higurashi, Higurashi, Higurashi, Higurashi Isomegusa, Aspergillus: Aspergillus, Pleurotus: Genus sylvestris, Asclepias: Fujimatsumo, Saw-toothed genus: Scutellaria japonicum: Genus Astragalus, Anemoneaceae, Anemoneaceae, Anemoneaceae, Anemoneaceae. Genus: Chinorimo, Genus Chisinori: Chisujinori, etc.).

Axial algae (genus Chara, genus Ciratham, genus Hociturimo: citrus limo, lycnotamnus, genus Flamus: himeflamus, chaboflask, tripera, etc.), yellow algae (genus Hikarimo: hikarimo, etc.) and the like.

In addition, as a material derived from an animal-based material, a chicken cob extract, cow or pig, human placenta extract, cow or pig stomach, duodenum, intestine, spleen extract or its decomposed product, cow or pig brain tissue Extract, collagen hydrolyzate of bovine/porcine or fish (acid, alkali, enzyme, etc.) or water-soluble collagen or collagen derivative such as acylated collagen, elastin of beef or pig or elastin hydrolyzate (acid, alkali, Enzymes, etc.) or water-soluble elastin derivatives, keratins and their degradation products or their derivatives, silk proteins and their degradation products or their derivatives, pig or bovine blood cell protein degradation products (globin peptides), cattle or pig hemoglobin degradation products (Hemin, hematin, heme, protoheme, heme iron, etc.), milk, casein and its decomposed products or their derivatives, skim milk powder and its decomposed products or their derivatives, lactoferrin or its decomposed products, chicken egg components, fish meat decomposed products, Nucleic acid-related substances (ribonucleic acid, deoxyribonucleic acid), etc.

As a material derived from a plant or animal material, any site, cell, tissue, organ, metabolite derived from a gene recombinant or a cell fusion can be used. Furthermore, for example, an undifferentiated cell group or a cell group in the process of being differentiated, which is obtained by culturing an arbitrary site, cell, tissue, organ or the like, can be used.

(14) Marine components Seawater such as deep sea water, for example, seawater salt, seawater dried matter, inorganic salts (sodium chloride, magnesium chloride, potassium chloride, etc.) obtained from the Dead Sea or the Atlantic Ocean or the Pacific Ocean, sea mud or mud ( Fango), for example, sea muddy or mud (containing components: silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, potassium oxide, etc.) in various places such as Italian fango, German fango, Eifelfango, and Freiburgfango. Magnesium oxide, calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese, arsenic, water), Seitoku stone, etc.

(15) Microorganism culture metabolites Yeast metabolites, yeast extract, bacterial metabolites, bacterial extract, fungi or actinomycete metabolites, mold or actinomycete extract, natto metabolites, natto extract, fermented rice extract , Fermented rice bran (red bran, Shiranuka), Euglena extract or its decomposition products or their water-soluble derivatives, trehalose or its derivatives, lactic acid fermented product of raw milk or skim milk powder, fermented product of lactic acid bacteria of legumes, coconut plant Fermented lactic acid bacteria, etc.

(16) α-hydroxy acids Glycolic acid, citric acid, malic acid, tartaric acid, lactic acid, etc.

(17) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin, bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, iron oxide yellow, Red iron oxide, black iron oxide, Gunjou, chromium oxide, chromium hydroxide, carbon black, calamine, etc.

(18) Ultraviolet absorbing/blocking agent Benzophenone derivative (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid sodium salt, dihydroxy Dimethoxybenzophenone, dihydroxydimethoxybenzophenone-sodium sulfonate, 2,4-dihydroxybenzophenone, tetrahydroxybenzophenone, etc., paraaminobenzoic acid derivative (paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, Octyl paradimethylaminobenzoate etc., methoxycinnamic acid derivatives (ethyl paramethoxycinnamate, isopropyl paramethoxycinnamate, octyl paramethoxycinnamate, 2-ethoxyethyl paramethoxycinnamate, sodium paramethoxycinnamate, potassium paramethoxycinnamate, diparamethoxycinnamic acid) Mono-2-ethylhexanoate glyceryl, etc.), salicylic acid derivatives (octyl salicylate, phenyl salicylate, homomenthyl salicylate, dipropylene glycol salicylate, ethylene glycol salicylate, myristyl salicylate, methyl salicylate, etc.), anthranilic acid derivatives (methyl anthranilate, etc.), Urocanic acid derivatives (urocanic acid, ethyl urocanate, etc.), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives , Nicotinic acid derivative, vitamin B6 derivative, umbelliferone, esculin, benzyl cinnamate, sinoxate, oxybenzone, dioxybenzone, octabenzone, thrisobenzone, benzoresorcinol, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, escarol. , Zinc oxide, talc, kaolin etc.

(19) Whitening agent Para-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound, benzotriazole derivative, tetrazole derivative, imidazoline derivative, pyrimidine derivative, dioxane derivative, camphor derivative, furan derivative, pyrone derivative, Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin C or its derivatives (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), vitamin E or its derivatives, kojic acid or its derivatives, oxybenzone, benzophenone, arbutin, guaiazulene, Shikonin, baicalin, baicalein, berberine, placenta extract, ellagic acid, rucinol, etc.

(20) Tyrosinase activity inhibitor Vitamin C or its derivative (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), hydroquinone or its derivative (hydroquinone benzyl ether, etc.), kojic acid or its derivative, vitamin E or its derivative, N-acetyltyrosine or its derivative, glutathione, hydrogen peroxide, zinc peroxide, placenta extract, ellagic acid, arbutin, lucinol, silk extract, plant extract (chamomile, mulberry, gardenia, tussock, oleander, clara, mugwort, honeysuckle) , Yellowfin, dokudami, matsuhodo, pearl barley, pearl oyster, hop, hawthorn, eucalyptus, yarrow, altea, keihi, mankeishi, hamamelis, yamagwa, life-prolonging grass, bellflower, toshishi, succulent, irrigation, mahuang, senkyu, dokatsu, rhinoceros , Bofu, Hamabofu, Ogon, Peony skin, Genshosho, Kuzukon, licorice, quintet, aloe, shouma, safflower, green tea, black tea, Asenyaku) etc.

(21) Melanin dye reducing/decomposing substance Phenyl mercury hexachlorophene, mercuric oxide, mercuric chloride, hydrogen peroxide solution, zinc peroxide, hydroquinone or its derivative (hydroquinone benzyl ether), etc.

(22) Turnover promoting action/cell activating substance Hydroquinone, lactic acid bacteria extract, placenta extract, ganoderma lucidum extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (aloe, (Osogon, horsetail, gentian, burdock, konkon, carrot, hamamelis, hop, quinine, deadline, senburi, touki, daffodil, armchair, gentius, cucumber, pearl millet, rosemary, parsley), etc.

(23) Astringent succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc paraphenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid (including catechin compound) and the like.

(24) Active oxygen scavenger SOD, catalase, glutathione peroxidase, etc.

(25) Antioxidant Vitamin C or its salt, stearic acid ester, vitamin E or its derivative, nordihydroguaselethenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxyanisole , Propyl gallate, sesamol, sesamolin, gossypol, propolis, etc.

(26) Lipid peroxide production inhibitor β-carotene, plant extract (cultured cells of sesame, armature, Hypericum perforatum, Hamamelis, clove, melissa, trillium, birch, salvia, rosemary, Nantenmi, ages, ginkgo, green tea) and the like.

(27) anti-inflammatory agent ictamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor, dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid or its Salt, glycyrrhetinic acid or a salt thereof, licorice extract, shikon extract, age extract, propolis and the like.

(28) Antibacterial/sterilizing/disinfecting agents Acrinol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercury chrome, lactoferrin or hydrolysates thereof, alkyldiaminoethylglycine chloride solution, triclosan, sodium hypochlorite, chloramine T, Salasi powder, iodine compound, iodoform, sorbic acid or its salt, propionic acid or its salt, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p -Chlorophenol, p-chloro-m-xylenol, p-chloro-m-cresol, thymol, phenethyl alcohol, O-phenylphenol, Irgasan CH3565, halocarbane, hexachlorophen, chlorohexidine, ethanol, methanol, isopropyl alcohol, benzyl alcohol , Ethylene glycol, propylene glycol, 2-phenoxyethanol, 1,2-pentanediol, zinc pyridione, chlorobutanol, isopropylmethylphenol, nonionic surfactants (polyoxyethylene lauryl ether, polyoxyethylene nonylphenyl ether, polyoxy Ethylene octyl phenyl ether, etc.), amphoteric surfactant, anionic surfactant (sodium lauryl sulfate, lauroyl sarcosine potassium, etc.), cationic surfactant (cetyltrimethylammonium bromide, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride), Formaldehyde, Hexamine, Brilliant Green, Malachite Green, Crystal Violet, Jermal, Photosensitizer No. 101, Photosensitizer No. 201, Photosensitizer No. 401, N-long chain acyl basic amino acid derivative and its acid addition salt, zinc oxide, hinokitiol, Kujin, Propolis, etc.

(29) Humectant Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfate or its salt, water-soluble chitin derivative, water-soluble chitosan derivative, pyrrolidonecarboxylic acid or its salt, lactic acid Sodium, mini-sasaniki extract, natto metabolite, natto extract, loofah water, birch (white birch) or red pine sap

(30) Inhibitor of elastase activity Diisopropyl fluorophosphate, plant extract (salmon, Hypericum, Clara, mulberry leaf, cinnamon bark, ginger ginger, comfrey, salvia, elderberry, bodige, button pipi), seaweed extract and the like.

(31) Hair agent Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thiantol, castari tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, sodium bromate, thio Glycolic acid etc.

(32) Antiandrogens Estrogen (estron, estradiol, ethinyl estradiol, etc.), isoflavone, oxendron, etc.

(33) Peripheral blood flow enhancer Vitamin E or a derivative thereof, assembly extract, garlic extract, ginseng extract, aloe extract, gentiana extract, touki extract, cepharanthin, carpronium chloride, minoxidil and the like.

(34) Stimulants Capsicum tincture, nonyl vanillamide, cantharis tincture, ginger tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.

(35) Metabolic activator Photosensitizer No. 301, hinokitiol, pantothenic acid or its derivatives, allantoin, placenta extract, biotin, pentadecanoic acid glyceride, etc.

(36) Antiseborrheic agent Pyridoxine or its derivative, sulfur, vitamin B6 and the like.

(37) Keratolytic agent resorcin, salicylic acid, lactic acid, urea, etc.

(38) Oxidizing agent hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium peroxytripolyphosphate, sodium bromate, potassium bromate, sodium pyrophosphate, Sodium peroxide orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate hydrogen peroxide adduct, sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract, etc.

(39) Hair remover Inorganic reducing agents such as strontium sulfate, sodium sulfide, barium sulfide and calcium sulfide, thioglycolic acid or salts thereof (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate) , Strontium thioglycolate) etc.

(40) Hair swelling agent: ethanolamine, urea, guanidine, etc.

(41) Dyeing agent 5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'-iminodiphenol, hydrochloric acid 2 ,4-diaminophenoxyethanol, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, metaphenylenediamine hydrochloride, orthoaminophenol , N-phenylparaphenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-diamine, nitroparaphenylenediamine , Paraaminophenol, paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol, picramic acid, sodium picramate, N,N'-bis(4-aminophenyl)-2,5-diamino-1,4-quinonediimine , 5-(2-hydroxyethylamino)-2-methylphenol, N-phenylparaphenylenediamine, metaaminophenol, metaphenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate, orthosulfate Aminophenol, orthochlorparaphenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, toluene-2,5-diamine sulfate, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroorthosulfate Phenylenediamine, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol, diphenylamine, α-naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, resorcin, Tannic acid, sodium 2-hydroxy-5-nitro-2',4'-diaminoazobenzene-5'-sulfonate, hematein and the like.

(42) Fragrances Natural animal flavors such as musk, civet, catrium, ambergris, anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, karaway essential oil, cardamom essential oil, guaiac wood essential oil, Cumin essential oil, black character essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, ginger grass essential oil, cedar essential oil, spearmint essential oil, western mint essential oil, Large cocoon oil, tuberose essential oil, clove essential oil, orange essential oil, winter green essential oil, true balsam essential oil, butchery essential oil, rose essential oil, palmarosa essential oil, cypress essential oil, hiba essential oil, sandalwood essential oil, petitgrain essential oil, bay essential oil, vetiver essential oil, bergamot Essential oils, Peruvian balsam essential oil, boad rose essential oil, scented essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil, Japanese mint essential oil and other plant flavors, etc. Synthetic fragrance, etc.

(43) Dyes/colorants Red cabbage dye, red rice dye, madder dye, annatto dye, squid dye, turmeric dye, enju dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye, onion dye , Tamarind pigment, spirulina pigment, whole buckwheat pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

(44) Other hormones, sequestering agents, pH adjusters, chelating agents, antiseptic/antifungal agents, cooling agents, stabilizers, emulsifiers, animal/vegetable proteins and their degradation products, animal/vegetable polysaccharides And its decomposed products, animal and plant glycoproteins and their decomposed products, blood flow promoters, anti-inflammatory/anti-inflammatory/anti-allergic agents, wound healing agents, foaming agents, thickeners, enzymes, purified water (electronic Water, including small clusters, etc.), oral agents, deodorants/deodorants, bitters, seasonings, etc. are added, and when used in combination with these, the effects of the present invention or other cosmetic effects are added. Or synergistic improvement can be expected.

The following can be mentioned as another aspect of the present invention.
Skin indigenous bacteria balance improver, Staphylococcus epidermidis growth promoter, Staphylococcus aureus growth inhibitor, Acne bacteria growth inhibitor, glycerol production promoter by Staphylococcus epidermidis, or in the production of antimicrobial peptide production promoter by epidermal cells, Use of Lactobacillus paracasei MCC1849 (NITE BP-01633).
Lactobacillus paracasei used to improve skin indigenous bacteria balance, promote Staphylococcus epidermidis growth, suppress Staphylococcus aureus growth, suppress acne bacteria growth, promote glycerol production by Staphylococcus epidermidis, or promote antibacterial peptide production by epidermal cells ( Lactobacillus paracasei) MCC1849 (NITE BP-01633).
Lactobacillus paracasei (Lactobacillus paracasei) for improving the balance of skin indigenous bacteria, promoting the growth of Staphylococcus epidermidis, suppressing the growth of Staphylococcus aureus, suppressing the growth of Acne, promoting the production of glycerol by Staphylococcus epidermidis, or promoting the production of antimicrobial peptides by epidermal cells paracasei) MCC1849 (NITE BP-01633).
The step of applying Lactobacillus paracasei MCC1849 (NITE BP-01633) to a mammal, or the skin indigenous bacterium balance improving agent, Staphylococcus aureus growth inhibitor, acne growth inhibitor of the present invention, acne A method for improving skin indigenous bacterial balance, a method for promoting Staphylococcus epidermidis growth promotion, including the step of applying a bacterial growth inhibitor, a glycerol production promoter by Staphylococcus epidermidis, or an antibacterial peptide production promoter by epidermal cells to a mammal A method for suppressing coccus growth, a method for suppressing acne growth, a method for promoting glycerol production by Staphylococcus epidermidis, or a method for promoting antibacterial peptide production by epidermal cells.
Examples of mammals include humans, cows, horses, goats, sheep, pigs, monkeys, dogs, cats, rats, mice, hamsters, guinea pigs, and the like. It is preferably human.

Moreover, the following can be mentioned as another aspect of this invention.
Use of Lactobacillus paracasei MCC1849 (NITE BP-01633) in the manufacture of a skin protectant.
Lactobacillus paracasei MCC1849 (NITE BP-01633) used for skin protection.
Lactobacillus paracasei MCC1849 (NITE BP-01633) for skin protection.
A method for protecting skin, comprising a step of applying Lactobacillus paracasei MCC1849 (NITE BP-01633) to a mammal, or a step of applying the skin protective agent of the present invention to a mammal.
Examples of mammals include humans, cows, horses, goats, sheep, pigs, monkeys, dogs, cats, rats, mice, hamsters, guinea pigs, and the like. It is preferably human.

Hereinafter, Examples using Lactobacillus paracasei MCC1849 in which the effect of the present invention was most remarkable will be shown, but the present invention is not limited to these Examples. In this example, "%" means "% by mass" unless otherwise specified.

(Deposition of microorganisms)
Lactobacillus paracasei MCC1849 was deposited on June 6, 2013 at the Patent Microorganism Depositary Center (Kazusa Kamazashi 2-5122, Kisarazu City, Chiba Prefecture) of the Japan Institute for Product Evaluation Technology, December 12, 2013. It was transferred to an international deposit under the Budapest Treaty on 19th March. The deposit number is NITE BP-01633.

Lactobacillus paracasei MCC1849 is not limited to the above-mentioned deposited bacterium and may be a lactic acid bacterium substantially equivalent to the above-mentioned deposited bacterium. The lactic acid bacterium substantially equivalent to the deposited bacterium is a lactic acid bacterium belonging to Lactobacillus paracasei, which promotes the growth of Staphylococcus epidermidis and suppresses the growth of Staphylococcus aureus and Acne bacterium, and the skin indigenous bacteria balance Lactobacillus that can improve the lactic acid bacteria, preferably promotes the production of glycerol by Staphylococcus epidermidis, prevents dry skin, regulates the pH of the surface of the skin to suppress the growth of bacteria, and also in the skin epidermal keratinocytes A lactic acid bacterium that promotes the production of human β-defensin-3, which is an antibacterial peptide. Further, in substantially equivalent lactic acid bacteria, the base sequence of the 16SrRNA gene is preferably 99.86% or more, more preferably 99.93% or more, more preferably the base sequence of the 16SrRNA gene of the deposited bacterium. Is a lactic acid bacterium having 100% homology and preferably having the same mycological properties as the above-mentioned deposited bacterium.

(Lactobacillus paracasei MCC1849 heat-treated killed cells)
Lactobacillus paracasei MCC1849 (NITE BP-01633) purchased from Morinaga Milk Industry Co., Ltd. was used.

(Lactobacillus casei ATCC 334, Lactobacillus paracasei ATCC BBA-52, Lactobacillus sakei ATCC 15521, Lactobacillus plantaran ATCC 8014 heat-treated dead cells)
Lactobacillus casei ATCC334, Lactobacillus paracasei ATCCBBA-52, Lactobacillus sakei ATCC15521, and Lactobacillus plantaran ATCC8014 were purchased from Kanto Kagaku. Each bacterium was cultured in 1 L of MRS medium (Kanto Kagaku) at 37° C. for 18 hours. The cells were collected by centrifugation at 3000 rpm for 20 minutes. The cells were washed twice with water and then dispersed in 100 mL of distilled water. After heating this bacterial cell dispersion at 100° C. for 10 minutes, the bacterial cells were collected by centrifugation and dried to obtain heat-treated dead bacterial cells.

(Production of plant polysaccharides)
As a plant polysaccharide, Aloe vera-derived polysaccharide was produced. After crushing 100 g of aloe vera leaves, squeeze extraction and filtration were performed, ethanol or isopropanol was added to the obtained filtrate and the mixture was stirred, and then the resulting precipitate was collected and washed again by adding ethanol or isopropanol. The washed product was dried to obtain an Aloe vera polysaccharide powder.

(Naturally derived polysaccharide)
Further, the above heat-treated killed cells, tamarind gum (DSP Gokyo Food & Chemical) derived from tamarind seeds, xanthan gum (DSP Gokyo Food & Chemical) and gellan gum (DSP) derived from extracellular components of microorganisms. Gokyo Food & Chemical), and cationized guar gum derived from leguminous plant gua seeds (DSP Gokyo Food & Chemical) were used as polysaccharides.

(Sample preparation 1)
Table 1 shows the heat-treated killed cells of Lactobacillus paracasei MCC1849 and Lactobacillus casei ATCC334, Lactobacillus paracasei ATCCBBA-52, Lactobacillus sakei ATCC15521, and Lactobacillus plantaran ATCC8014. Samples 1-7 were prepared by diluting with the medium to the final concentration.

(Sample preparation 2)
Lactobacillus paracasei MCC1849 heat-treated killed cells were mixed with aloe vera polysaccharide, tamarind gum, xanthan gum, gellan gum, and cationized guar gum to prepare samples 8-19 (final concentration of medium addition) shown in Table 2.

(Sample preparation 3)
Lactobacillus paracasei ATCCBBA-52 heat-treated killed cells were mixed with aloe vera polysaccharide, tamarind gum, xanthan gum, gellan gum, and cationized guar gum to prepare samples 20-31 (final concentration of medium addition) shown in Table 3.

(Cultivation of Staphylococcus epidermidis standard bacteria)
As a culture medium, Nutribillon No. 2 (Nissui Pharmaceutical Co., Ltd.) was used. Samples 1-3 (Table 1 values: medium final concentration %) were added to this medium. Next, Staphylococcus epidermidis standard bacteria (ATCC 12228) were inoculated into the medium at 1-5×10 ³ CFU/μL (CFU: colony forming unit), and these were cultured at 37° C. for 24 hours. As a control, heat-treated dead cells were not added (Sample 1).

(Results of colony formation measurement of Staphylococcus epidermidis standard bacteria)
The results are shown in Figure 1. By adding 0.1-0.5% of killed cells of Lactobacillus paracasei MCC1849 heat-treated, colony formation of Staphylococcus epidermidis standard bacteria (ATCC 12228) was increased about 9-10 times compared to the control (FIG. 1).

(Cultivation of Staphylococcus aureus standard bacteria)
As a culture medium, Nutribillon No. 2 (Nissui Pharmaceutical Co., Ltd.) was used. Samples 1-3 (Table 1 values: medium final concentration %) were added to this medium. Next, Staphylococcus aureus standard bacteria (ATCC 6538) were inoculated into the medium at 1-5×10 ³ CFU/μL, and these were cultured at 37° C. for 24 hours. As a control, heat-treated dead cells were not added (Sample 1).

(Results of colony formation measurement of Staphylococcus aureus standard bacteria)
The results are shown in Figure 2. Lactobacillus paracasei MCC1849 heat-treated killed cells addition of 0.1% reduced Staphylococcus aureus standard bacteria (ATCC 6538) colony formation by about 2.8%, and addition of 0.5% decreased by about 7.5%. (Figure 2).

(Method for collecting human skin indigenous bacteria)
A 3 cm square between the eyebrows of the test subject was rubbed with a sterile cotton swab three times in the longitudinal direction and three times in the lateral direction to collect skin-resident bacteria. Then, the swab was washed in 10 mL of phosphate buffered saline to collect the bacteria.

(Culture of human skin indigenous bacteria)
As a culture medium, Nutribillon No. 2 (Nissui Pharmaceutical Co., Ltd.) was used. Samples 1-3 (Table 1 values: medium final concentration %) were added to this medium. Then, 100 μL medium was inoculated with the skin indigenous bacteria collected by the above method. These were cultured at 37° C. for 24 hours. As a control, heat-treated dead cells were not added (Sample 1).

(Results of human colonization of Staphylococcus epidermidis)
Results are shown in FIG. The addition of 0.1% heat-killed Lactobacillus paracasei MCC1849 cells increased the colonization of Staphylococcus epidermidis in humans by about 5 times, and by adding 0.5%, it increased 7-8 times (FIG. 3).

(Human acne colony formation measurement results)
The results are shown in Fig. 4. Addition of 0.1% heat-killed Lactobacillus paracasei MCC1849 cells reduced human colonization of Acne bacteria by approximately 15%, and by addition of 0.5% by approximately 25% (FIG. 4).

(Ratio of colonization number of Staphylococcus epidermidis and Acne bacterium among human skin indigenous bacteria)
Results are shown in FIG. The proportion of each bacterium was calculated by setting the total number of colony formation of Staphylococcus epidermidis and Acne bacterium as 100. By adding heat-treated killed cells of Lactobacillus paracasei MCC1849 to a culture solution of skin-resident bacteria collected from the human eyebrow, the ratio of Staphylococcus epidermidis increases in a concentration-dependent manner, and the ratio of Acne bacteria increases. Decreased (Fig. 5).

(Cultivation of Staphylococcus epidermidis standard bacteria)
As a culture medium, Nutribillon No. 2 (Nissui Pharmaceutical Co., Ltd.) was used. Samples 1, 2, and 4-7 (Table 1 values: medium final concentration %) were added to this medium. Next, Staphylococcus epidermidis standard bacteria (ATCC 12228) were inoculated into the medium at 1-5×10 ³ CFU/μL (CFU: colony forming unit), and these were cultured at 37° C. for 24 hours. As a control, heat-treated dead cells were not added (Sample 1).

(Results of colony formation measurement of Staphylococcus epidermidis standard bacteria)
Results are shown in FIG. The Lactobacillus paracasei MCC1849 heat-treated killed cells were compared with the heat-treated killed cells of Lactobacillus casei ATCC334, Lactobacillus paracasei ATCCBBA-52, Lactobacillus sakei ATCC15521, and Lactobacillus plantaran ATCC8014. , Significantly increased colony formation of Staphylococcus epidermidis (ATCC 12228) (FIG. 6). In particular, about 3-fold increase was observed for the same genus Lactobacillus paracasei ATCCBBA-52, and about 2-fold increase was observed for Lactobacillus sakai ATCC15521.

(Measurement of glycerol amount)
As a culture medium, Nutribillon No. 2, the killed cells of Lactobacillus paracasei MCC1849 heat-treated were added to this medium at a final concentration of 0.25, 0.5, 1 w/w%. Next, Staphylococcus epidermidis standard bacteria (ATCC 12228) was inoculated into the medium at 1-5×10 ³ CFU/μL, and these were cultured at 37° C. for 24 hours. The control was Lactobacillus paracasei MCC1849 with no heat-treated dead cells (Sample 1), and each culture solution was collected. These cultures were vortexed, and the amount of glycerol was measured by a glycerol assay kit (Cayman Chemical) according to a conventional method.

(Measurement result of glycerol amount)
The results are shown in Fig. 7. Lactobacillus paracasei MCC1849 heat-treated killed cells significantly increased glycerol production from S. epidermidis standard strain (ATCC 12228) (FIG. 7). At the final addition concentration of 1.0 w/w% to the medium, an approximately 4-fold glycerol production promoting effect was observed.

(Measurement of glycerol amount)
As a culture medium, Nutribillon No. 2, using Lactobacillus paracasei MCC1849, Lactobacillus casei ATCC334, Lactobacillus paracasei ATCCBBA-52, Lactobacillus sakei ATCC15521, and Lactobacillus plantaran ATCC8014 to a final concentration of 1.0. % Was added. Next, Staphylococcus epidermidis standard bacteria (ATCC 12228) was inoculated into the medium at 1-5×10 ³ CFU/μL, and these were cultured at 37° C. for 24 hours. As a control, heat-treated killed cells were not added, and each culture solution was collected. These cultures were vortexed, and the amount of glycerol was measured by a glycerol assay kit (Cayman Chemical) according to a conventional method.

(Measurement result of glycerol amount)
The results are shown in Fig. 8. The Lactobacillus paracasei MCC1849 heat-treated killed cells were compared with the heat-treated killed cells of Lactobacillus casei ATCC334, Lactobacillus paracasei ATCCBBA-52, Lactobacillus sakei ATCC15521, and Lactobacillus plantaran ATCC8014. , Significantly increased glycerol production from Staphylococcus epidermidis (ATCC 12228) (FIG. 8). The Lactobacillus paracasei MCC1849 heat-treated killed cells showed a glycerol production promoting effect of about 5 times, while the other killed cells only had a 1-2 times promoting effect.

(Quantitative analysis of human β-defensin-3)
Human epidermal keratinocytes (Kurabo) were cultured to subconfluence in a 24-well plate using a medium for epidermal cells (Humedia KG2, Kurabo). After culturing in a differentiation-inducing medium containing calcium added to Humidia KG2 for 3 days at 37° C. in the presence of 5% CO ₂ , the medium was replaced with a new medium. Lactobacillus paracasei MCC1849 heat-treated killed cells were added to this medium to a final concentration of 0.05 and 0.1 w/w%. As a positive control, Staphylococcus epidermidis standard bacteria were added at about 1×10 ⁴ cells (mL)/mL. After culturing for 24 hours, the culture supernatant was collected. Human β-3 Defensin-3 was quantified using the Human BD-3 Standard ABTS ELISA Development Kit (PeproTech).

(Results of quantitative analysis of human β-defensin-3)
The results are shown in Fig. 9. Lactobacillus paracasei MCC1849 heat-treated killed cells also increased the expression level of β-defensin-3 from human epidermal keratinocytes (FIG. 9).

(Quantitative analysis of human β-defensin-3)
In a 24-well plate, human epidermal keratinocytes were cultured to subconfluence using the medium for epidermal cells as described above. After culturing in a differentiation-inducing medium containing calcium added to Humidia KG2 for 3 days at 37° C. in the presence of 5% CO ₂ , the medium was replaced with a new medium. In this medium, Lactobacillus paracasei MCC1849 heat-treated dead cells were added to a final concentration of 0.1 w/w%, and Lactobacillus casei ATCC334 and Lactobacillus paracasei were added to a final concentration of 0.1 w/w%. Heat-treated dead cells of ATCC BBA-52, Lactobacillus sackei ATCC 15521, and Lactobacillus plantaran ATCC 8014 were added. After culturing for 24 hours, the culture supernatant was collected. Human β-3 Defensin-3 was quantified using the Human BD-3 Standard ABTS ELISA Development Kit (PeproTech).

(Results of quantitative analysis of human β-defensin-3)
The results are shown in Fig. 10. The Lactobacillus paracasei MCC1849 heat-treated killed cells were compared with the heat-treated killed cells of Lactobacillus casei ATCC334, Lactobacillus paracasei ATCCBBA-52, Lactobacillus sakei ATCC15521, and Lactobacillus plantaran ATCC8014. , Significantly increased the expression level of β-defensin-3 from human epidermal keratinocytes (FIG. 10). In particular, an expression level of 4.5 times that of the same genus Lactobacillus paracasei ATCCBBA-52 was observed.

(Cultivation of Staphylococcus epidermidis standard bacteria in the presence of plant-derived or microbial-derived polysaccharides)
As a culture medium, Nutribillon No. 2 (Nissui Pharmaceutical Co., Ltd.) was used. Sample 9-19 (Table 2 value: medium final concentration %) was added to this medium. Next, Staphylococcus epidermidis standard bacteria (ATCC 12228) was inoculated into the medium at 1-5×10 ³ CFU/μL, and these were cultured at 37° C. for 24 hours. The control was Lactobacillus paracasei MCC1849 with no heat-treated dead cells (Sample 8).

(Results of measuring colony formation of Staphylococcus epidermidis standard plant-derived or microbial-derived polysaccharide)
The results are shown in Fig. 11. Colonization of Staphylococcus epidermidis standard bacteria (ATCC 12228) by heat-treated killed Lactobacillus paracasei MCC1849 is synergistically enhanced by the combined use of aloe vera polysaccharide, tamarind gum, xanthan gum, gellan gum, and cationized guar gum. (Fig. 11).

(Cultivation of Staphylococcus epidermidis standard bacteria in the presence of plant-derived or microbial-derived polysaccharides)
As a culture medium, Nutribillon No. 2 (Nissui Pharmaceutical Co., Ltd.) was used. To this medium, samples 21-31 (Table 3 values: medium final concentration %) were added. Next, Staphylococcus epidermidis standard bacteria (ATCC 12228) was inoculated into the medium at 1-5×10 ³ CFU/μL, and these were cultured at 37° C. for 24 hours. The control was Lactobacillus paracasei ATCCBBA-52 with no heat-treated dead cells (Sample 20).

(Results of measuring colony formation of Staphylococcus epidermidis standard plant-derived or microbial-derived polysaccharide)
Results are shown in FIG. Colonization of Staphylococcus epidermidis standard bacteria (ATCC 12228) by heat-killed Lactobacillus paracasei ATCC BBA-52 is enhanced by the combined use of aloe vera polysaccharide, tamarind gum, xanthan gum, gellan gum, and cationized guar gum. Although a trend was seen, it was not a significant synergistic effect as seen with Lactobacillus paracasei MCC1849 heat-treated killed cells (Figure 12).

(Production example of various external preparations for skin protection)
1. Sunscreen cream (O/W cream type)
(Oil phase part)
2-Methylhexyl paramethoxycinnamate 5.0%
4-t-butyl-4'-methoxydibenzoylbutane 1.0%
Squalane 10.0%
Vaseline 5.0%
Stearyl alcohol 3.0%
Stearic acid 3.0%
Glyceryl monostearate 3.0%
Polyacrylic acid 1.0%
Antioxidant Proper amount Preservative Proper amount Perfume Proper amount Lactobacillus paracasei MCC1849 Heat-treated dead cells
0.01-1% (appropriate amount)
(Water phase part)
Purified water 100% residual dipropylene glycol 7.0%
Titanium dioxide (alumina surface treated product) 5.0%
Disodium edetate 0.05%
Triethanolamine 1.0%
(Manufacturing method)
The oil phase part and the water phase part are respectively heated to 70° C. to dissolve them, and the titanium dioxide is sufficiently dispersed in the water phase part. It was filtered, cooled, and stored. As a cooling method, a method of gradually cooling while agitating while a refrigerant was injected into the jacket of the production kettle, a method of cooling using a heat exchanger, and the like were used.

2. Sunscreen emulsion (W/O type emulsion)
(Oil phase part)
2-Methylhexyl paramethoxycinnamate 5.0%
Titanium dioxide (hydrophobized product) 5.0%
Zinc oxide (hydrophobized product) 9.0%
Squalane 20.0%
Silicone oil 23.0%
Silicone resin 2.0%
Glycerin diisostearate 2.0%
Organically modified montmorillonite 0.5%
Preservative Suitable amount Perfume Suitable amount Lactobacillus paracasei MCC1849 Heat-treated dead cells
0.01-1% (appropriate amount)
(Water phase part)
Purified water 100% Residual 1,3-butylene glycol 5.0%
(Manufacturing method)
The oil phase part and the water phase part were each heated to 60° C. to be dissolved, and the oil phase part was sufficiently dispersed with titanium dioxide and zinc oxide. Then, the mixture was degassed, filtered, cooled, and stored.

3. Sun oil Paramethoxy 2-ethylhexyl cinnamate 2.0%
Liquid paraffin 56.0%
Isopropyl myristate 10.0%
Silicone oil 30.0%
Silicone resin 2.0%
Antioxidant Proper amount Perfume Proper amount Lactobacillus paracasei MCC1849 Heat-treated dead cells
0.01-1% (appropriate amount)
(Manufacturing method)
The silicone resin was added to the silicone oil and dissolved, and then isopropyl myristate was added and thoroughly mixed with stirring, and then the remaining components were added and stirred.

4. Powder type foundation (powder)
Zinc myristate treated talc 20.0%
Sericite 34.6%
Titanium oxide 10.0%
Red iron oxide 1.0%
Yellow iron oxide 4.0%
Black iron oxide 0.4%
Titanium oxide/aluminum oxide surface treated mica 15.0%
Nylon powder 5.0%
Lactobacillus paracasei MCC1849 heat-treated killed cells
0.01-1% (appropriate amount)
(Base material)
Squalane 3.0%
Octyldodecyl myristate 3.5%
Vaseline 2.5%
(Other)
Preservative Suitable amount Perfume Suitable amount (Production method)
The powder components were mixed using a mixer such as a high-speed flow type mixer, and then fine particles were formed using a pulverizer. Then, a base material was added and thoroughly mixed using a vertical screw type mixer. Further, after crushing and granulating using a hammer mill or the like, compression molding was performed.

5. Dual-use type foundation (powder)
Silicon treated titanium oxide 10.0%
Talc 30.0%
Sericite 39.6%
Bengal 1.5%
Yellow iron oxide 5.5%
Black iron oxide 0.4%
Methyl methacrylate powder 3.0%
Lactobacillus paracasei MCC1849 heat-treated killed cells
0.01-1% (appropriate amount)
(Base material)
Squalane 3.0%
Dimethylpolysiloxane 2.0%
Glycerin triisooctanoate 3.0%
Ethyl paraaminobenzoate 2.0%
(Other)
Preservative Suitable amount Perfume Suitable amount (Production method)
The powder components were mixed using a mixer such as a high-speed flow type mixer, and then fine particles were formed using a pulverizer. Then, a base material was added and thoroughly mixed using a vertical screw type mixer. Further, after crushing and granulating using a hammer mill or the like, compression molding was performed.

6. Cream (W/O type)
(Phase A)
t-butyl methoxydibenzoylmethane 3.0%
Diethylaminohydroxybenzoyl hexyl benzoate 2.0%
Dimethicone, caprylyl dimethicone ethoxyglucoside 20.0%
Tri(behenic acid/isostearic acid/eicosandioic acid) glyceryl 1.0%
Stearyl dimethicone 1.0%
Tocopherol acetate 0.05%
Benzoic acid 0.3%
Diisopropyl sebacate 7.0%
Octocrylene 8.0%
Talc, yellow iron oxide, Mg stearate, titanium dioxide, mica, Al hydroxide, stearic acid 1.4%
(Phase B)
Titanium dioxide, Mg stearate, talc, black iron oxide, Al hydroxide, mica, stearic acid 1.13%
Red and brown iron oxide, Mg stearate, titanium dioxide, mica, water, Al oxide, stearic acid 0.85%
Titanium dioxide, talc, magnesium stearate, Al hydroxide, mica, stearic acid 3.9%
(Phase C)
Residual sulfuric acid Mg 0.7% with water 100
Betaine 1.0%
Hyaluronic acid Na 0.1%
Glycerin 1.0%
(Phase D)
Phenoxyethanol 0.4%
(E phase)
Titanium dioxide, glycerin, alumina 3.5%
(F phase)
Dimethylsilylated silica 1.0%
(G phase)
Cyclopentasiloxane, (dimethicone/vinyltrimethylsiloxysilicic acid) crosspolymer 1.0%
Lactobacillus paracasei MCC1849 heat-treated killed cells
0.01-1% (appropriate amount)
(H phase)
Fragrance 0.3%
(Manufacturing method)
The raw materials for phase A were mixed with stirring, the temperature was raised to 70 to 75° C., phase B was added to phase A with stirring, and the mixture was stirred until it became uniform. In a separate container, mix the raw materials of phase C with stirring, raise the temperature to 70-75°C, add phase C to the AB mixed phase, slowly add while stirring (about 5 minutes), and add 3 minutes after adding phase C. Stir and then allow to cool. When the temperature reached 40°C or lower, phase D was added and stirred for about 1 minute, phase E was added and stirred for about 5 minutes, then phase F was added and stirred for about 1 minute, and phase G was added and stirred for about 1 minute. Finally, H phase was added and stirred for about 2 minutes, and then naturally cooled to room temperature.

7. Moisturizing gel (beauty essence)
(Phase A)
Olive oil fatty acid cetearyl + olive oil fatty acid sorbitan 3.5%
Unsaved olive oil 0.5%
Dicaprylyl carbonate 3.0%
Shea butter 0.5%
Wheat germ oil 0.5%
Cyclomethicone 6.0%
Dimethicone 1.0%
(Phase B)
Residual glycerin with water 100% 1.5%
Propylene glycol 1.5%
Polyacrylic acid Na 0.7%
Xanthan gum 0.1%
(Phase C)
Preservative Suitable amount Perfume Suitable amount Menthol 0.3%
Ethanol 3.0%
Lactobacillus paracasei MCC1849 heat-treated killed cells
0.01-1% (appropriate amount)
(Manufacturing method)
Phases A and B were mixed and prepared. Disperse xanthan gum and Na polyacrylate using a batch type disperser, etc., add phase A to phase B at 75°C, homogenize for a few minutes, cool slowly with stirring, and add phase C at 40°C. (Dissolve menthol in ethanol).

8. Lip balm (Phase A)
Mineral oil 8.0%
Red pigment 1.0%
Yellow pigment 0.3%
Lactobacillus paracasei MCC1849 heat-treated killed cells
0.01-1% (appropriate amount)
(Phase B)
Tri(caprylic acid/capric acid) glyceryl 100 and residual ethylhexyl methoxycinnamate 3.0%
Isopropyl myristate 10.0%
(Phase C)
Alkyl (C26-28) methicone 9.0%
Cetanol 1.6%
(Phase D)
PEG-8, tocopherol, ascorbyl palmitate, ascorbic acid, citric acid mixture 0.1%
Propylparaben 0.1%
(E phase)
Perfume 0.2%
Mixture of mica and titanium oxide 18.4%
Mixture of mica, titanium oxide and iron oxide 7.0%
Silica 0.5%
(Manufacturing method)
Phase A is uniformly premixed, phase B is added and mixed evenly, phase C is added, and the mixture is slowly stirred and heated to 78-80°C, then phase D and phase E are added little by little and stirred uniformly. Then, it was poured into a mold and naturally cooled to room temperature.

9. Liquid foundation (O/W type)
(Oil phase)
Stearic acid 2.4%
Propylene glycol monostearate 2.0%
Cetostearyl alcohol 0.2%
Liquid lanolin 2.0%
Liquid paraffin 3.0%
Isopropyl myristate 8.5%
Oil-soluble preservative Amount (water phase)
Purified water 100% residual sodium carboxymethyl cellulose 0.2%
Bentonite 0.5%
Dipropylene glycol 4.0%
Triethanolamine 1.1%
Water-soluble preservative Suitable amount (powder)
Titanium oxide 8.0%
Talc 4.0%
Coloring pigment Lactobacillus paracasei MCC1849 Heat treated dead cells
0.01-1% (appropriate amount)
(Other)
Fragrance suitable amount (manufacturing method)
After pulverizing the powder, an aqueous phase was added and mixed, and an oil phase and a fragrance were added thereto with stirring, and the mixture was emulsified and dispersed by a homomixer, and then cooled to room temperature.

10. Cream foundation (W/O type)
(Oil phase)
Decamethylcyclopentasiloxane 15.0%
Liquid paraffin 5.0%
Paramethoxycinnamic acid ester 3.0%
Polyether-modified dimethyl siloxane 2.0%
(Water phase)
Purified water 100% residual glycerin 5.0%
Water-soluble preservative Suitable amount (powder)
Titanium oxide 10.0%
Talc 3.8%
Coloring pigment Lactobacillus paracasei MCC1849 Heat treated dead cells
0.01-1% (appropriate amount)
(Other)
Fragrance suitable amount (manufacturing method)
After pulverizing the powder, an aqueous phase was added and mixed, and an oil phase and a fragrance were added thereto with stirring, and the mixture was emulsified and dispersed by a homomixer, and then cooled to room temperature.

11. Moisturizing cream (O/W type)
(Phase A)
Shea butter 1.5%
Alkyl benzoate (C12-15) 1.3%
Alkyl (C26-28) dimethicone 6.5%
Glyceryl stearate 1.5%
Glyceryl stearate, PEG-100 stearate mixture 3.0%
Hexyl laurate 1.3%
Isododecane 1.3%
Isohexadecane 1.3%
Isopropyl palmitate 1.3%
Tocopherol acetate 0.5%
Lactobacillus paracasei MCC1849 heat-treated killed cells
0.01-1% (appropriate amount)
(Phase B)
Purified water 100% residual carbomer 0.3%
Glycerin 2.0%
Propylene glycol 2.0%
(Phase C)
Fragrance 0.5%
Phenoxyethanol, methylparaben, ethylparaben, butylparaben, propylparaben, isobutylparaben mixture 0.5%
Tetrahydroxypropyl ethylenediamine 0.7%
(Manufacturing method)
After heating the A phase and the B phase in separate containers to 70° C., the A phase was added to the B phase, homogenized using a batch type disperser, and naturally cooled to 40° C. with stirring. Then, phase C was added to this and further homogenized, and cooled to room temperature.

12. Skin cream (quasi drug)
(Phase A)
Mineral oil 10.00%
Isopropyl myristate 16.00%
(Phase B)
Polysorbate 60 3.00%
Buritylene glycol 3.00%
Phenoxyethanol 0.50%
Water 33.80%
(Phase C)
Water 30.00%
Ascorbyl phosphate Mg 3.00%

(Manufacturing method of skin cream)
Phase A was dissolved with stirring. Phase B was heated to 50° C. with stirring and dissolved. Phase C was heated to 50° C. with stirring and dissolved. While stirring Phase A, Phase B was added and emulsified at 50° C. (Phase D). While stirring phase D, phase C was added and dissolved. The product was cooled to room temperature.

13. Human moisturizing test with moisturizing cream (subject)
The subjects were 17 people who satisfied the following matters. The average age was 42.0 ± 10.7 years.
1) Japanese women aged 20 to 60 at the time of obtaining test consent
2) Those who feel the bulk of the skin
3) Those who are concerned about skin color and dullness
4) Those who have poor makeup and are likely to lose makeup
5) Persons who can change the facial cleansing foam and cream that they usually use during the test period to test products.
6) Regarding skin care during the test period, those who can agree to use only the lotion that they normally use and the cream of the test product, and stop using other creams, emulsions, gels, serums, masks, etc.

The following persons were excluded from the subjects.
1) Pregnant or possibly pregnant, and lactating
2) Those who are allergic to cosmetics
3) Those who have experience in aesthetic medicine that affects the test site
4) Those who participate in other clinical trials

The following items were set as items to be managed by the subjects.
1) Sleep 6 hours or more the day before the test.
2) Avoid outdoor leisure during the test period, and take UV protection measures to prevent sunburn, such as hats and parasols.
3) During the test period, change the face wash foam and lotion that are normally used to the test product.
4) For skin care during the test period, use only the lotion that is normally used and the test cream, and stop using other creams, emulsions, gels, serums, masks, etc.
5) Avoid eating or drinking irritants that may affect the skin condition during the test period.
6) During the test period, it is prohibited to drink special care such as facial beauty treatment and supplements for the purpose of beautiful skin.

(Facial cleansing foam ingredients)
Water 90.944%
Ethanol 5.000%
Potassium stone base 3,000%
Cocamidopropyl betaine 0.900%
Na chloride 0.156%

(Method of manufacturing face wash foam)
Kariishi Ken base was finely grated. It was melted in a hot water bath, and once dissolved, the other ingredients were mixed.

(Ingredients for moisturizing cream (test product))
(Phase A)
Water 67.1975%
Jojoba seed oil 15.0000%
Propanediol 10.0000%
Polyglyceryl oleate-2 2.0000%
Sucrose distearate 2.0000%
Hydrogenated jojoba oil 1.0000%
(Phase B)
Phenoxyethanol 1.0000%
Behenyl alcohol 1.0000%
(Phase C)
Hydrogenated lysolecithin 0.2000%
(Phase D)
Hydroxypropyl methylcellulose stearoxy ether
0.2000%
BG 0.1500%
Tocopherol 0.1000%
(E phase)
Lactobacillus paracasei MCC1849 heat-treated killed cells
0.05 or 0.1%
(F phase)
Fragrance 0.0500%
Aloe vera leaf extract 0.0025%

(Manufacturing method of moisturizing cream (test product))
Phases A were mixed and dissolved by heating at 80 to 85°C. After allowing Phase B to blend in, Phase C was added and dissolved by heating at 45 to 50° C., Phase D was added and stirred until Phase E was uniformly dispersed. Then, it heated to 80-85 degreeC. The phase B was stirred with a homomixer while being heated, and the phase A was added thereto to emulsify it. Then, cooling was started while stirring at a low speed, and when the temperature became 60° C. or lower, the phase F was charged respectively, and after uniformly dispersing, cooling and low speed stirring were continued until the temperature reached 30° C.

(Ingredients for moisturizing cream (control product))
The components of the above-mentioned moisturizing cream (test product) were the same as those of the above-mentioned moisturizing cream (test product) except that Lactobacillus paracasei MCC1849 heat-treated killed cells were not added.

(Manufacturing method of moisturizing cream (control product))
The same method was used for manufacturing the moisturizing cream (test product).

(Test method)
As a test subject, the control product was used for 1 week before the test product was used, and the test product was used for 4 weeks thereafter. In addition, when the test product was used for the test subject, 0.5 g of the test product was applied to the face after washing the face with the above-mentioned face washing foam. In each case, the test article was applied once a night.
The skin water content of the subject was measured 1 week after using the control product and 4 weeks after using the test product. Before the measurement, the subjects washed their faces and acclimated for 20 minutes in a constant temperature and constant humidity room. After that, continuously, in the same room, the skin water content (arbitrary unit, au) was measured at three places on each of the right cheek and left cheek using Corneometer CM825 (Courage+Khazaka), and each of the right cheek and left cheek was measured. The average of the measured values was calculated. The measurement was performed according to the product manual. The results are shown in Table 4. In addition, each numerical value is the average value±standard deviation of the skin water content of the subjects (17 persons).
From Table 4, it was found that the skin water content of the test subject after 4 weeks using the test product was significantly larger than that when using the control product.

The heat-treated killed Lactobacillus paracasei MCC1849 cells according to the present invention promote the growth of Staphylococcus epidermidis, which is a useful skin-resident bacterium, and suppress the growth of harmful bacteria Staphylococcus aureus and Acne. Also, in a preferred form, it promotes the production of glycerol derived from Staphylococcus epidermidis. Furthermore, it promotes the production of antimicrobial peptides from epidermal cells. Therefore, it is extremely useful as a cosmetic or a skin protector for normalizing or maintaining the normal skin condition.

Claims (8)

Lactobacillus paracasei (Lactobacillus paracasei) MCC1849 (NITE BP-01633) is a skin indigenous bacteria balance improving agent containing an active ingredient. A skin protective agent containing Lactobacillus paracasei MCC1849 (NITE BP-01633) as an active ingredient. The skin protective agent according to claim 2, which is a Staphylococcus epidermidis growth promoter. The skin protective agent according to claim 2, which is a Staphylococcus aureus growth inhibitor. The skin protective agent according to claim 2, which is an acne bacterium growth inhibitor. The skin protective agent according to claim 2, which is an agent for promoting glycerol production by Staphylococcus epidermidis. The skin protector according to claim 2, which is an antibacterial peptide production promoter by epidermal cells. A cosmetic containing Lactobacillus paracasei MCC1849 (NITE BP-01633).