KR102362055B1 - A cosmetic composition for skin improvement comprising polysaccharides, yeast extracts and fermentation of strain having probiotics properties - Google Patents

Abstract

The present invention relates to a cosmetic composition containing polysaccharides, yeast extract, and a fermented product of a strain having probiotic properties as active ingredients, which is safe for the human body and has the effect of improving the condition of the skin.

Description

{A cosmetic composition for skin improvement comprising polysaccharides, yeast extracts and fermentation of strain having probiotics properties}

The present invention relates to a composition for external application for skin improvement comprising polysaccharides, yeast extract, and a fermented product of a strain having probiotic properties as active ingredients.

As people's living standards improve, interest in health as well as beauty increases day by day, efforts to maintain beautiful skin are becoming common, and various cosmetic compositions for skin care are being provided.

From this cosmetic point of view, hair occupies an important part. Hair is a thin, keratinized structure produced on the surface of the skin. It serves as a cushion against external shock, protects the human body from external stimuli such as direct sunlight, cold, friction, and danger, and discharges heavy metals such as arsenic, mercury, and zinc that are harmful to the body. Aspects are also highlighted.

It is known that aggravation of hair condition including hair loss can be caused by various causes, including changes in diet or increase in internal and external stress, but generally, the scalp and hair are mutually dependent, and the condition of the scalp affects the hair. known as one of the largest factors (Int. J. Trichology., 2018 Nov-Dec; 10(6): 262-270)

On the other hand, external composition for skin, including scalp and hair care, is provided in various forms in terms of raw material, form, and function, and most of them add artificial compounds to kill harmful bacteria in the skin or control oil content. Although the compound has excellent functional advantages, it sometimes irritates the skin and causes damage to the skin in the long run.

Therefore, by improving the problems of these existing external composition for application to the skin, the development of a composition that is safe for the human body and can effectively improve the condition of the skin is being demanded.

The present invention was created to solve the problems of the prior art as described above, and an object of the present invention is to contain plant-derived polysaccharides, yeast-derived extracts, and fermented strains having probiotic properties as active ingredients, thereby promoting beneficial bacteria or harmful bacteria on the skin. It is to provide a cosmetic composition for skin improvement that can exhibit a reducing effect.

The present invention provides a cosmetic composition for skin improvement comprising, as an active ingredient, a yeast extract as a polysaccharide, a microorganism-derived extract, and a fermented product of a strain having probiotic properties as a microorganism-derived fermented product.

As used herein, the term "probiotics" refers to microorganisms that exist in the human body and can have beneficial effects on human health and the above actions, and metabolites such as dead cells and extracts and fermented products of the microorganisms. means all inclusive. "Prebiotics" refers to food that can be used for the proliferation, growth promotion and activity enhancement of the microorganisms.

As used herein, the term “microflora” refers to a cell group of microorganisms that are normally present in a specific part of the human body and can affect human health.

In the present invention, the fermented strain having probiotic properties by itself did not show a significant effect of improving the skin condition by enhancing or reducing beneficial bacteria, but, together with the strain fermented product, polysaccharides that are probiotics and yeast as other probiotics By confirming that the composition further comprising the derived extract can exhibit prebiotic properties that can promote the growth and proliferation of beneficial bacteria, the composition is provided as a composition for improving scalp or skin condition.

In the present invention, the polysaccharide may be at least one selected from the group consisting of inulin, beta-glucan and maltodextrin. The polysaccharides as described above contribute to the enhancement of immunity by improving the activity of immune cells in the human body, and have the effect of reducing oxidative stress in cells.

Inulin is a polysaccharide used as a means for storing energy in many plants, such as chicory, wheat, onion, banana, garlic, and asparagus, and can generally be obtained from the root or rhizome of the plant. When inulin is applied to the skin, inulin can protect microorganisms with prebiotic properties existing in the skin from antibacterial substances. Inulin represented by the following Chemical Formula 1 (C _6n H _10n+2 O _5n+1 ) includes a glucosyl moiety at the chain end and a repeating fructosyl moiety therebetween.

[Formula 1]

The n may be 2 to 60, but is not limited thereto. In the present invention, inulin can be obtained from chicory root, but it can also be purchased and used commercially at home and abroad.

Beta-glucan is a polysaccharide formed in the cell walls of grains, bacteria and fungi. When beta-glucan is applied to the skin, beta-glucan has the effect of increasing the antioxidant activity and anti-wrinkle activity in skin cells, and improving UV protection, wound healing and moisturizing power. Beta-glucan represented by the following Chemical Formula 2 includes a repeating glucosyl moiety that is glycosidic-bonded at the beta 1 and 3 positions.

[Formula 2]

The n may be 2 to 100, but is not limited thereto. In the present invention, beta-glucan can be obtained by extracting from the mushroom fruit body, but it can also be purchased and used in domestic and foreign markets.

Maltodextrin is a polysaccharide formed by partial hydrolysis of vegetable starch. When maltodextrin is applied to the skin, maltodextrin increases antioxidant activity in skin cells. Maltodextrin represented by the following formula (3) includes a repeating glucosyl moiety that is glycosidic-linked at the alpha 1, 4 position.

[Formula 3]

The n may be 2 to 20, but is not limited thereto. In the present invention, it can be obtained by hydrolyzing corn or wheat starch, but commercially available products can be purchased and used at home and abroad.

In the present invention, the yeast extract is an extract obtained by extracting yeast with a solvent, a fraction obtainable by hydrolyzing yeast cells, a culture medium of yeast and yeast in which cultures coexist, the above-mentioned fraction or an extract extracted from the culture medium, the above-mentioned fraction Alternatively, it may mean encompassing a filtrate obtained by filtering yeast from a culture medium, a diluted solution or a dried product of the above-mentioned fraction or extract, and the like. For example, the yeast extract may be a fraction that can be separated by a solvent after extracting yeast with a pharmaceutically acceptable organic solvent, or hydrolyzing yeast cells using an enzyme.

In the present invention, the yeast extract may be at least one selected from the group consisting of brewer-derived yeast extract and truffle-derived yeast extract.

The brewer-derived yeast extract may be an extract obtained by culturing the yeast of the genus Saccharomyces corresponding to the ascomycete, or extracting the sterilized culture after purchasing and culturing the yeast of the same species. Preferably, the yeast may be brewer's yeast ( Saccharomyces cerevisiae ). Brewer's yeast is also called baker's yeast, and is used in the manufacture of alcoholic beverages including beer and bread. The brewer's yeast extract can be obtained by fermenting the wort in the beer manufacturing process using brewer's yeast, filtering the beer, and then drying the separated yeast. Brewer's yeast extract is non-fermentable and contains a large amount of minerals such as carbohydrates, proteins, nucleic acids, B vitamins and phosphorus. Vitamin B group has the effect of improving skin health and hair condition. In the present invention, it can be obtained by isolating and culturing the yeast used in the beer manufacturing process, and then sterilizing it, but it can also be purchased and used in domestic and foreign markets.

Truffle (truffle)-derived yeast extract is an extract obtained by isolating and culturing yeast among microorganisms present in truffle, or purchasing and culturing yeast of the same species as the yeast found in truffle, followed by sterilization. can Truffle-derived yeast extract contains a large amount of beta-glucan and a small amount of B vitamins. In the present invention, the extract can be obtained by the method exemplified above, but can also be purchased and used in domestic and foreign markets.

In the present invention, the strain having probiotic properties may be one or more anaerobic strains selected from the group consisting of Lactobacillus sp. strain and Bifidobacterium sp. strain. Therefore, the fermented product of the strain having probiotic properties may mean encompassing the fermentation metabolites of the strain, but is not limited thereto.

In the present invention, the fermented material is not only the fermented material obtainable from the strain, but also the culture medium of the strain and the culture coexisting, the fermented product obtained by filtering the strain from the culture medium, and sterilizing the strain from the culture medium and The filtered fermented product, the above-mentioned fermented product or an extract extracted from a culture medium containing the same, a diluted solution or dried product of the above-mentioned fermented product or extract, and a lysate obtained by collecting and crushing the cells of the strain, etc. may be included. Preferably, fermentation can mean fermentation lysate and fermentation filtrate.

For example, fermentation may encompass fermentation lysate and fermentation filtrate. Fermentation lysate means a product obtained by killing the corresponding bacteria by using conditions such as heat, pH, enzyme, and pressure after fermentation by anaerobic strains. Fermentation lysates contain not only fermentation metabolites, but also various useful components within the strain. Fermentation filtrate means a supernatant obtained by removing microorganisms including the corresponding strain through a separation method such as filtration after fermentation by anaerobic strains.

Lactobacillus genus ( Lactobacillus ) The strain is a gram-positive bacteria that is distributed throughout the natural environment, such as inside the human body or plants and soil. Lactobacillus sp. strains can produce lactic acid through fermentative metabolism for hexoses. The Lactobacillus sp. strain can suppress the growth of harmful bacteria including specific pathogens by producing lactic acid or hydrogen peroxide as a result of metabolism in the human body.

In the present invention, the Lactobacillus sp. strain includes a homozygous lactic acid fermentation strain that ferments one hexose molecule into two lactic acid molecules, and a heterozygous lactic acid fermentation strain that additionally produces ethanol, acetic acid, carbon dioxide, etc. as well as lactic acid molecules from hexose molecules. can mean inclusive. For example, Lactobacillus sp. strains, Lactobacillus acidophilus ( L. acidophilus ), Lactobacillus del Bruekii ( L. delbruekii ), Lactobacillus helveticus ( L. helveticus ), Lactobacillus salivarius ( L ) salivarius ), Lactobacillus casei ( L. casei ), Lactobacillus plantarum ( L. plantarum ), Lactobacillus Sakei ( L. sakei ), Lactobacillus brevis ( L. brevis ), Lactobacillus buchner ( L bunchneri ), Lactobacillus fermentum ( L. fermentum ), Lactobacillus reuteri ( L. reuteri ), Lactobacillus rhamnosus ( L. rhamnosus ), Lactobacillus paracasei ( L. paracasei ), Lactobacillus johnsonni ( L Johnsonii ), Lactobacillus vulgaris ( L. bulgaris ) and the like may include, but are not limited thereto. Preferably, the Lactobacillus sp. strain may be at least one of Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus fermentum, Lactobacillus paracasei and Lactobacillus vulgaris. More preferably, the Lactobacillus sp. strain may be at least one of Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus fermentum and Lactobacillus paracasei. Most preferably, the Lactobacillus sp. strain may be at least one of Lactobacillus acidophilus and Lactobacillus plantarum.

Therefore, in the present invention, the fermented product of the Lactobacillus sp. strain may include the fermentation metabolite of the strain for hexoses, and preferably may be a fermentation lysate obtained by killing the strain.

Bifidobacterium genus ( Bifidobacterium ) The strain is a gram-positive bacteria distributed throughout the natural environment, such as the human skin, food, and soil. The Bifidobacterium sp. strain is a heterogeneous lactic acid fermentation strain that produces lactic acid, acetic acid, formic acid, etc. through a unique hexasaccharide fermentation metabolism using fructose-6-phosphate phosphoketolase.

Bifidobacterium sp. strain in the present invention, for example, Bifidobacterium animalis ( B. animalis ), Bifidobacterium bifidum ( B. bifidum ), Bifidobacterium breve ( B. breve ), Bifidobacterium dentium ( B. dentium ), Bifidobacterium longum ( B. longum ), Bifidobacterium pseudolongum ( B. pseudolongum ) and the like may be included, but are not limited thereto. Preferably, the Bifidobacterium sp. strain may be at least one of Bifidobacterium animalis, Bifidobacterium bifidum and Bifidobacterium longum.

In the present invention, the fermented product of the Bifidobacterium sp. strain may include the fermentation metabolite of the strain for hexoses, and preferably may be a fermentation lysate obtained by killing the strain.

The cosmetic composition for improving skin according to an embodiment of the present invention contains inulin, beta-glucan, maltodextrin, brewer's yeast extract, truffle-derived yeast extract, Lactobacillus fermentation lysate or Bifidobacterium fermentation lysate as an active ingredient can do.

Preferably, the cosmetic composition may include inulin, beta-glucan, maltodextrin, brewer's yeast extract, truffle-derived yeast extract, Lactobacillus fermentation lysate and Bifidobacterium fermentation lysate in the same ratio. For example, the cosmetic composition comprises inulin, beta-glucan, maltodextrin, brewer's yeast extract, truffle-derived yeast extract, Lactobacillus fermentation lysate and Bifidobacterium fermentation lysate in order a:b:c:d: It may be included in a weight ratio of e:f:g. Here, a to g may each be 1 to 10, preferably a to g may each be 1 to 5, and most preferably a to g may all be 1.

The cosmetic composition for skin improvement according to an embodiment of the present invention is for inulin, beta-glucan, maltodextrin, brewer's yeast extract, truffle-derived yeast extract, Lactobacillus fermentation lysate and Bifidobacterium fermentation based on 100% by weight of the total. One or more active ingredients selected from the group consisting of seafood may be included in an amount of 0.00001 to 10% by weight. Preferably, it may be included in an amount of 0.0001 to 1% by weight, more preferably 0.0005 to 0.5% by weight, and most preferably 0.001 to 0.1% by weight.

As used herein, the term “skin improvement” may include not only improvement in skin condition observed with the naked eye, but also include factors that directly and indirectly affect skin health.

Specifically, the cosmetic composition for skin improvement according to the present invention may be for improving the microbial flora inhabiting the skin. The improvement of the microbial flora may include increasing beneficial bacteria by promoting the growth of beneficial bacteria present in the skin, or reducing harmful bacteria by inhibiting the growth of harmful bacteria.

Specifically, the cosmetic composition for skin improvement according to the present invention may be any one of skin soothing, skin wrinkle improvement, and skin elasticity improvement. Skin soothing may encompass preventing or normalizing disorders on the skin, including preventing or inhibiting inflammation in the skin. Skin wrinkle improvement or elasticity improvement may include preventing or suppressing damage to the dermal layer or volume reduction caused by natural factors such as aging and external environmental factors such as UV rays and oxidative stress.

Specifically, the cosmetic composition for skin improvement according to the present invention may be for application to the scalp. For example, the cosmetic composition for improving the scalp may be any one for scalp soothing, scalp oil improvement, hair loss prevention, and hair growth improvement. Skin soothing can mean preventing or inhibiting inflammation in the scalp. Improving scalp oil may mean reducing the amount of oil secretion when excess oil is secreted from the skin. Prevention of hair loss or improvement of hair growth may mean increasing the activity of dermal papilla cells of the scalp, inhibiting inflammatory factors for cells constituting the skin, or increasing the rate of collagen biosynthesis in cells constituting the skin.

Preferably, "skin improvement" may mean an increase in beneficial bacteria present in the skin and a decrease in harmful bacteria. The beneficial bacteria and harmful bacteria are classified based on the effect of the strain on the skin health of the human body, and for example, there is a strain of the genus Staphylococcus , but is not limited thereto.

For example, among the strains of the genus Staphylococcus, bacteria such as Staphylococcus epidermidis , also known as Staphylococcus epidermis, do not act as pathogens for normal skin, but produce antibacterial peptides against other pathogens or It can be classified as beneficial bacteria because it has the effect of inhibiting the progression of some cancers.

For example, bacteria such as Staphylococcus aureus , also known as Staphylococcus aureus among strains of the genus Staphylococcus, can cause various skin infections while present on the skin surface or pores, and can cause disease even on normal skin. It can cause bacterium and can be classified as harmful bacteria. When the skin is the scalp, harmful bacteria may include Aspergillus niger ( A. niger ), which can cause inflammation in the scalp by producing mycotoxins.

As will be described later, the composition according to an embodiment of the present invention can provide an improvement effect on the skin by improving the flora in the human body, such as by increasing beneficial bacteria.

Human dermal papilla cells are mesenchymal cells present in scalp hair follicles and play a decisive role in controlling the events of hair generation and hair growth cycle. Excessive presence of oil in the scalp may cause dermatitis, and inflammatory factors may induce an inflammatory response to cells present in the scalp. Collagen refers to a protein constituting the connective tissue present in the extracellular matrix, and when the biosynthesis rate of collagen in the scalp decreases, it may cause weakening of the dermis of the scalp and shrinkage of hair follicles accordingly.

As will be described later, the composition according to an embodiment of the present invention provides an improvement effect on the skin by increasing the activity of specific cells, reducing the oil content of the skin, inhibiting the production of inflammatory factors in the cells of the skin, and increasing the rate of collagen biosynthesis can do.

As used herein, the term "cosmetic composition" refers to a composition for administration by direct application or spraying to the skin. The term "administration" used in the present invention means introducing the composition according to the present invention to a target tissue using a conventional method known in the art.

The cosmetic composition for skin improvement according to the present invention is a lotion, facial lotion, body lotion, nourishing cream, moisture cream, eye cream, essence, cosmetic ointment, spray, gel, pack, sunscreen, makeup base, foundation, powder, cleansing cream , cleansing lotion, cleansing oil, cleansing foam, soap or body wash formulation.

In the cosmetic composition for skin improvement according to the present invention, when the skin is scalp or hair, hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition Cream, hair moisture cream, hair massage cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservative, hair dye, hair wave agent, hair bleach, hair gel , hair glaze, hair dresser, hair lacquer, hair moisturizer, hair mousse or hair spray.

When the composition of the present invention is formulated in a liquid form, the composition of the present invention may include a carrier for the active ingredient. The carrier is, for example, water, ethanol, castor oil, glycerin, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbate. It may be a fatty acid ester such as hearth, but is not limited thereto. These may be used alone or in combination of two or more.

When the composition of the present invention is formulated as a paste, cream or gel, the composition of the present invention may include a carrier for the active ingredient. The carrier is, for example, animal oil, vegetable oil, wax, paraffin, starch, cellulose derivatives such as tracanth, hydroxyethyl, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, cetostearyl alcohol or chloride It may be stearyltriethylammonium, but is not limited thereto. These may be used alone or in combination of two or more.

When the composition of the present invention is formulated as a powder or spray, the composition of the present invention may include a carrier for the active ingredient. The carrier may be, for example, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder, but is not limited thereto. These may be used alone or in combination of two or more. When the composition of the present invention is formulated as a spray, a propellant such as chlorofluorohydrocarbon, propane, butane or dimethyl ether may be further included.

When the composition of the present invention is formulated as a soap, the composition of the present invention may include a carrier for the active ingredient. The carrier may be, for example, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, an aliphatic alcohol, vegetable oil, glycerol, sugar, etc., but is limited thereto. it is not going to be These may be used alone or in combination of two or more.

The composition of the present invention may further include additives commonly used for cosmetic compositions. The additive may be, for example, purified water, a surfactant, a moisturizer, a lower alcohol, a chelating agent, a disinfectant, an antioxidant, a preservative, a color, or a fragrance, but is not limited thereto. These may be used alone or in combination of two or more.

Above, all components included in the present invention do not exceed the regulations set by each country. Preferably, the cosmetic composition of the present invention may contain the mentioned individual components within a range that does not exceed the maximum amount stipulated in the "Cosmetics Safety and Technical Code" set by the Chinese government.

The cosmetic composition for skin improvement according to the present invention contains polysaccharides as prebiotics and yeast-derived extracts and strain fermented products as probiotics, compared to when prebiotics or probiotics are applied alone, growth of beneficial bacteria and harmful bacteria in the skin A good effect on inhibition was achieved. Such a cosmetic composition is harmless to the human body, and has an effect of improving the skin condition through improvement of the skin flora.

Specifically, the cosmetic composition for improving skin according to the present invention has an effect of improving scalp health and hair condition by increasing the activity of dermal papilla cells.

In addition, the cosmetic composition for skin improvement according to the present invention has the effect of improving the skin condition by inhibiting the generation of inflammatory factors for skin cells.

In addition, the cosmetic composition for improving skin according to the present invention has the effect of improving the condition and health of the skin by increasing the collagen biosynthesis rate in skin cells.

In addition, the cosmetic composition for skin improvement according to the present invention has an effect of improving the oil-water balance on the skin surface by removing foreign substances and oil without adverse effects on the skin.

1 is a photograph of the scalp before (a) application and (b) after one application of a hair shampoo according to an embodiment of the present invention.
2 is a photograph of the scalp before (a) application and (b) after one application of the hair shampoo according to an embodiment of the present invention.
3 is a photograph of the scalp before (a) application and (b) after one application of the hair shampoo according to an embodiment of the present invention.

The objects, specific advantages and novel features of the present invention will become more apparent from the following detailed description and preferred embodiments. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples. In addition, in describing the present invention, if it is determined that a detailed description of a related known technology may unnecessarily obscure the gist of the present invention, the detailed description thereof will be omitted.

manufacturing example. Complex containing polysaccharide, yeast extract, and fermented strain with probiotic properties

Commercially available products were used for inulin (Beneo), beta-glucan (QGen Biotech Co., Ltd.), maltodextrin (Samyang), and brewer's yeast extract (Active-On) used in the Examples below. For the truffle-derived yeast extract, an extract obtained by separating yeast from truffle and culturing at 37° C. for 48 hours, followed by sterilization, was used. Lactobacillus fermentation lysate and Bifidobacterium fermentation lysate were used as extracts obtained by sterilizing Lactobacillus and Bifidobacterium (Chr. Hansen) after culturing at 37° C. for 48 hours.

As (Preparation Example 1) , a complex was prepared in which inulin, brewer's yeast extract, and lactobacillus fermentation lysate were mixed by 10 g each. As (Preparation Example 2) , a complex was prepared in which beta-glucan, brewer's yeast extract, and lactobacillus fermentation lysate were mixed by 10 g each. As (Preparation Example 3) , a complex was prepared in which maltodextrin, brewer's yeast extract, and lactobacillus fermentation lysate were mixed by 10 g each. (Preparation Example 4) A complex was prepared in which inulin, truffle-derived yeast extract, and Bifidobacterium fermentation lysate were mixed by 10 g each. As (Preparation Example 5) , a complex was prepared in which beta-glucan, truffle-derived yeast extract, and Bifidobacterium fermentation lysate were mixed by 10 g each. As (Preparation Example 6) , a complex was prepared in which maltodextrin, truffle-derived yeast extract, and 10 g each of the fermentation lysate of Bifidobacterium were mixed. As (Preparation Example 7) , inulin, beta-glucan, maltodextrin, brewer-derived yeast extract, truffle-derived yeast extract, Lactobacillus fermentation lysate, and Bifidobacterium fermentation lysate each 10g were mixed to prepare a complex.

Example. Cosmetic composition containing polysaccharide, yeast extract, and fermented product of a strain having probiotic properties as an active ingredient

Example 1-1. hair shampoo composition

A hair shampoo composition having a composition as shown in Table 1 below was prepared.

Ingredient name content
(weight %) Ingredient name content
(weight %) Disodium Laureth
sulfosuccinate 5.000 inulin 0.001 lauryl hydroxysultaine 5.000 beta glucan 0.001 Disodium Lauryl Sulfosuccinate 1.000 maltodextrin 0.001 glycerin 1.000 brewer's yeast extract 0.001 caprylyl glycol 1.000 Truffle-derived yeast extract 0.001 Tetrasodium EDITA 0.500 Lactobacillus Ferment Lysate 0.001 Spices appropriate amount Bifidobacterium fermentation lysate 0.001 pH adjuster appropriate amount Purified water remaining amount antiseptic appropriate amount total 100

Example 1-2. hair shampoo composition

A hair shampoo composition having a composition as shown in Table 2 below was prepared.

Ingredient name content
(weight %) Ingredient name content
(weight %) Disodium Laureth
sulfosuccinate 10.000 inulin 0.001 Cocamidopropyl Betaine 5.000 beta glucan 0.001 glycerin 1.000 maltodextrin 0.001 caprylyl glycol 1.000 brewer's yeast extract 0.001 Polyquaternium-7 0.500 Truffle-derived yeast extract 0.001 Spices appropriate amount Lactobacillus Ferment Lysate 0.001 pH adjuster appropriate amount Bifidobacterium fermentation lysate 0.001 antiseptic appropriate amount Purified water remaining amount total 100

Example 2. Hair treatment composition

A hair treatment composition having a composition as shown in Table 3 below was prepared.

Ingredient name content
(weight %) Ingredient name content
(weight %) ethanol 1.000 inulin 0.001 cetearyl alcohol 20.000 beta glucan 0.001 Stearamidopropyl
Dimethylamine 5.000 maltodextrin 0.001 shea butter 5.000 brewer's yeast extract 0.001 Macadamia Seed Oil 5.000 Truffle-derived yeast extract 0.001 PEG-100 Stearate 5.000 Lactobacillus Ferment Lysate 0.001 Polyquaternium-7 0.500 Bifidobacterium fermentation lysate 0.001 pH adjuster appropriate amount Spices appropriate amount antiseptic appropriate amount Purified water remaining amount total 100

Example 3. Hair tonic

A hair tonic composition having a composition as shown in Table 4 below was prepared.

Ingredient name content
(weight %) Ingredient name content
(weight %) ethanol 30.000 Octyldodeces-16 0.200 Thief's Wand Root Extract 0.005 inulin 0.001 niacinamide 0.100 beta glucan 0.001 menthol 0.100 maltodextrin 0.001 chilli fruit extract 0.050 brewer's yeast extract 0.001 Benzylnicotinate 0.005 Truffle-derived yeast extract 0.001 foot root extract 0.300 Lactobacillus Ferment Lysate 0.001 Spices appropriate amount Bifidobacterium fermentation lysate 0.001 pH adjuster appropriate amount Purified water remaining amount total 100

Example 4. Body Wash

A body wash composition having a composition as shown in Table 5 below was prepared.

Ingredient name content
(weight %) Ingredient name content
(weight %) Trisodium EDIT 0.050 inulin 0.001 Disodium Cocoyl Glutamate 4.000 beta glucan 0.001 Sodium Cocoyl Alaninate 3.000 maltodextrin 0.001 Lauramidopropyl Betaine 3.000 brewer's yeast extract 0.001 PEG-40 Hydrogenated
castor oil 1.000 Truffle-derived yeast extract 0.001 Spices appropriate amount Lactobacillus Ferment Lysate 0.001 pH adjuster appropriate amount Bifidobacterium fermentation lysate 0.001 antiseptic appropriate amount Purified water remaining amount total 100

Example 5. Body Lotion

A body lotion composition having a composition as shown in Table 6 below was prepared.

Ingredient name content
(weight %) Ingredient name content
(weight %) glycerin 8.000 inulin 0.001 caprylic/capric
triglycerides 2.000 beta glucan 0.001 Cetylethylhexanoate 2.000 maltodextrin 0.001 Glyceryl Stearate 2.000 brewer's yeast extract 0.001 cetearyl alcohol 1.000 Truffle-derived yeast extract 0.001 Acrylate/C10-30 Alkylacrylate Crosspolymer 0.100 Lactobacillus Ferment Lysate 0.001 Disodium EDIT 0.020 Bifidobacterium fermentation lysate 0.001 pH adjuster appropriate amount Spices appropriate amount antiseptic appropriate amount Purified water remaining amount total 100

Experimental Example 1. Confirmation of effect on growth of beneficial bacteria

In order to confirm the effect of the complex of Preparation Example on the growth of beneficial bacteria, the beneficial bacteria were Staphylococcus epidermidis ( S. epidermidis ), and the harmful bacteria were Staphylococcus aureus ( S. aureus ). Before use, the bacteria were stored at -80°C, and a plurality of 100 mL of tryptic soy broth (TSB) medium was prepared, and 500 μl of the stock of each bacteria was inoculated for each medium. After rotation at 37 °C for 17 hours at a speed of 210 rpm, 4 mL of the culture solution was scooped out, inoculated into 40 mL of TSB, and subcultured at 37 °C for 8 hours at a speed of 210 rpm.

After 8 hours, the optical density (O.D., optical density) value was adjusted to 0.65, and the composites prepared according to Preparation Examples 1 to 7 were inoculated at a concentration of 1%, respectively, and the initial optical density value was measured. Thereafter, the optical density was measured after rotational incubation at a speed of 210 rpm for 16 hours at 37°C. The difference between the optical density measurement value and the initial measurement value after 16 hours for each medium was standardized and evaluated by dividing the initial measurement value.

As a comparative example, inulin, beta-glucan, maltodextrin, brewer-derived yeast extract, truffle-derived yeast extract, Lactobacillus fermentation lysate and Bifidobacterium fermentation lysate were each inoculated at a concentration of 1%. As a control group, an untreated group that was not treated with anything was used.

The inoculation results of the Preparation Examples and Comparative Examples were compared to the untreated group, and the average degree of increase or decrease of beneficial bacteria and harmful bacteria was converted into percentages, and the results are shown in Table 7 below.

division Beneficial bacteria ( S. epidermidis ) Harmful bacteria ( S. aureus ) Lactobacillus Ferment Lysate -9.90 21.42 Bifidabacterium fermentation lysate -4.69 26.00 brewer's yeast extract 2.48 16.37 Truffle-derived yeast extract 3.39 14.95 inulin 0.29 -0.84 beta glucan -0.26 -0.78 maltodextrin 0.14 1.43 Preparation Example 1 5.51 -2.46 Preparation 2 4.79 -2.88 Preparation 3 3.88 -2.31 Preparation 4 7.18 -2.49 Preparation 5 6.07 -2.71 Preparation 6 5.99 -2.19 Preparation 7 15.54 -7.47

As shown in Table 7, inulin, beta-glucan, and maltodextrin were polysaccharides with prebiotic properties, but did not show significant effects on the increase of beneficial bacteria and reduction of harmful bacteria alone, and fermentation lysate alone with probiotic properties Rather, it was found that beneficial bacteria decreased and harmful bacteria increased.

In contrast, the composites according to Preparation Examples 1 to 7 of the present invention showed significantly superior effects in increasing beneficial bacteria and reducing harmful bacteria compared to Comparative Examples in which polysaccharides, yeast extract or fermentation lysate were used alone, prebiotics It was confirmed that when yeast extract and fermentation lysate were used together as polysaccharides and probiotics, there was a synergistic effect on increasing beneficial bacteria and reducing harmful bacteria. In particular, it was confirmed that Preparation Example 7 exhibits the most excellent effect on the increase in beneficial bacteria and increase in harmful bacteria compared to other Preparation Examples.

Experimental Example 2. Confirmation of activity of dermal papilla cells

In order to confirm the effect of the complex of Preparation Example on dermal papilla cells, 4-8 passage human dermal papilla cells (Promocell, C-12071) were used as the cell line, and follicle dermal papilla cell growth media (Promocell, C) was used as the initial culture medium. -26501) was used.

Cells were seeded at a level of 3,000 to 6,000 cells per well in a 96-well plate, and cultured for 24 hours in an incubator at 37°C and a carbon dioxide concentration of 5%. Thereafter, the culture medium was replaced with DMEM medium supplemented with 0.1% of fetal bovine serum (FBS) and cultured for 24 hours in an incubator at 37°C and a carbon dioxide concentration of 5%. Thereafter, the culture medium was replaced with DMEM containing 0.1% FBS treated with the complexes according to Preparation Examples 1 to 7, and cultured for 48 hours in an incubator at 37° C. and a carbon dioxide concentration of 5%. After processing 10 μl of CCK-8 solution per well, incubating at 37° C. for 1 hour, optical density at 450 nm was measured.

The difference between the optical density measurement value and the initial measurement value after 48 hours for each well was standardized and evaluated by dividing the difference by the initial measurement value, and the average value is shown in Table 8 below. As a control, minoxidil 1 μM was treated. Significant difference was derived by assuming equal variance (significant difference p<0.05).

density average Preparation Example 1 1 ppm 5.8 10 ppm 7.7 Preparation Example 2 1 ppm 5.6 10 ppm 7.1 Preparation 3 1 ppm 5.2 10 ppm 8.1 Preparation 4 1 ppm 4.8 10 ppm 7.7 Production Example 5 1 ppm 6.1 10 ppm 10.2 Preparation 6 1 ppm 5.9 10 ppm 8.8 Preparation 7 1 ppm 16.9 10 ppm 21.5 minoxidil 1 μM 44.2

As shown in Table 8, it was confirmed that the complexes according to Preparation Examples 1 to 7 of the present invention had a synergistic effect on dermal papilla cell activity. In particular, it was confirmed that Preparation Example 7 exhibits the most excellent effect on the increase in dermal papilla cell activity compared to other Preparation Examples.

Experimental Example 3. Confirmation of inhibition of inflammatory factors

In order to confirm the effect of the complex of Preparation Example on the production of NO as an inflammatory factor in cells, a mouse Raw 264.7 cell line is used, and NaOH is added to Complete RPMI (RPMI:FBS:Antibiotic = 10:1:0.1) as an initial culture medium. Thus, a culture solution adjusted to a final pH of 8.5 was used.

Incubated for 24 hours at 37 ° C., 5% carbon dioxide concentration, when Raw 264.7 cells appeared on the culture medium, seeded in a 24-well plate prepared with DMEM culture, 37 ° C, carbon dioxide concentration 5% condition 24 incubated for hours. Thereafter, the complexes according to Preparation Examples 1 to 7 were diluted according to the following concentrations while replacing the medium with a serum-free DMEM culture medium, and then treated with 1 μg/mL LPS. After culturing for 24 hours at 37° C. and a carbon dioxide concentration of 5%, the supernatant was mixed with Griess reagent reagent (Sigma-Aldrich) 1:1 to evaluate the ability to inhibit NO production by absorbance, and the average value is shown in Table 9 below. :

NO production inhibition ability (%) = {1 - (NO production amount when compound is added / NO production amount in untreated group)} × 100

As a control, 20 μg/mL of L-NMMA1 (N ^G -Methyl-L-arginine acetate salt), an NO inhibitor, was used.

density average Preparation Example 1 One % 2.2 7% 10.8 Preparation 2 One % 3.1 7% 7.5 Preparation 3 One % 3.5 7% 8.1 Preparation 4 One % 2.8 7% 9.4 Preparation 5 One % 3.3 7% 8.7 Preparation 6 One % 3.3 7% 7.5 Preparation 7 One % 5.7 7% 23.3 L-NMMA1 20 μg/mL 52.1

As shown in Table 9, it was confirmed that the complexes according to Preparation Examples 1 to 7 of the present invention had the ability to inhibit NO production as an inflammatory factor in cells. In particular, it was confirmed that Preparation Example 7 exhibited the most excellent NO production inhibitory ability compared to other Preparation Examples.

Experimental Example 4. Confirmation of collagen biosynthesis rate

The effect of the complex of Preparation Example on the rate of collagen biosynthesis in the human skin fibroblasts was confirmed. Skin fibroblasts were cultured for 72 hours in complete DMEM culture medium at 37° C. and 5% carbon dioxide concentration. After seeding in a 24-well plate, it was incubated for 24 hours at 37°C and a carbon dioxide concentration of 5%. After treating the complex according to Preparation Example and culturing for 48 hours at 37°C and 5% carbon dioxide concentration, only the supernatant was separated, and the collagen synthesis effect was confirmed by absorbance using a procollagen type I C-peptide (PIP) EIA kit. . The increase rate of collagen biosynthesis was evaluated as follows, and the average value is shown in Table 10 below:

Collagen biosynthesis increase rate (%) = {(absorbance in complex treated group - absorbance in untreated group)/absorbance in untreated group}×100

As a control, 10 ng/mL of the cytokine TGF-β was used.

density average Preparation Example 1 One % 8.7 7% 10.8 Preparation 2 One % 8.6 7% 11.5 Preparation 3 One % 7.1 7% 10.8 Preparation 4 One % 9.5 7% 12.2 Preparation 5 One % 10.3 7% 15.4 Preparation 6 One % 8.5 7% 11.1 Preparation 7 One % 15.0 7% 35.5 TGF-β 10 ng/mL 36.6

As shown in Table 10, it was confirmed that the composites according to Preparation Examples 1 to 7 of the present invention had an effect of increasing collagen biosynthesis in fibroblasts. In particular, it was confirmed that Preparation Example 7 exhibits the most excellent effect on the increase in collagen biosynthesis in fibroblasts compared to other Preparation Examples.

Experimental Example 5. Confirmation of improvement in scalp oil

The effect of improving scalp oiliness when using the hair shampoo composition according to Example 1-1 was confirmed. As the test subjects, 22 adult women aged 20 to 50 years were selected, and the test subject's scalp was selected as the test site. The test subject was prohibited from using other products such as shampoos, treatments, and hair essences, and from hair treatments such as dyeing and perms during this experiment.

After allowing the test subject to rest for 30 minutes in a constant temperature and humidity room at 22±2° C. and 50±5% humidity, the experiment was performed, and all experiments were performed in the constant temperature and humidity room. After the test subject sufficiently wet the hair and scalp with lukewarm water, the same amount of the hair shampoo composition according to Example 1-1 was evenly applied to the hair and scalp, massaged, and then rinsed thoroughly with running water.

The evaluation of scalp oil before and after using the hair shampoo was performed using a Sebumeter (SKIN-O-MAT, Cosmomed GmbH) and a video microscope (Kong PC Camera, Bomtech). A probe cassette with an oil adsorption tape was placed on the crown of all the test subjects, and the oil was sufficiently absorbed by contact with the same pressure for 30 seconds, and then the cassette was inserted into the body of the Sebumeter to measure the amount of sebum. In addition, under the same lighting conditions, the parietal part of all subjects was observed at 300 times magnification using a video microscope.

Hereinafter, statistical processing in this experiment was analyzed using SPSS 17.0 for Windows. Table 11 below shows the results showing the amount of sebum (μg/cm ² ) before and after using the composition of Example 1-1 (the maximum amount of sebum measurable with a Sebumeter is 350 μg/cm ² ).

before use Immediately after 1 use average 200.41 32.05 Standard Deviation 37.03 14.87

Table 12 below shows the results of paired t-test analysis ( ^* p<.05, ^** p <.01, ^{* **} p<.001): improvement rate (%) = {(measured value after 1 use - measured value before use)/measured value before use} × 100

Sebum improvement rate p-value Immediately after 1 use 84.01 .000 ^***

In addition, individual scalp conditions and changes in the scalp condition before and after use of the composition of Example 1-1 were surveyed, and the results are shown in Tables 13 and 14 below, respectively (N = total frequency = number of subjects = 22 ).

question frequency percentage(%) The scalp is clean with no wastes or foreign substances not at all 2 9.1 Not like that 19 86.4 is average One 4.5 Yes 0 0.0 it really is 0 0.0 The scalp is fresh without excessive sebum secretion. not at all 2 9.1 Not like that 19 86.4 is average One 4.5 Yes 0 0.0 it really is 0 0.0 Sum 22 100.0

question frequency percentage(%) It seems that wastes and foreign substances on the scalp are thoroughly cleaned not at all 0 0.0 Not like that 0 0.0 is average One 4.5 Yes 20 91.0 it really is One 4.5 The scalp seems refreshed by reducing excess sebum not at all 0 0.0 Not like that 0 0.0 is average 2 9.1 Yes 16 72.7 it really is 4 18.2 Sum 22 100.0

Additionally, when using the composition of Examples, the results are shown in Table 15 below (0: none, 1: mild, 2: moderate, 3: severe).

adverse reaction Immediately after 1 use adverse reaction Immediately after 1 use Erythema (redness) 0 stinging (pain) 0 Edema (swelling) 0 burning sensation 0 scale (keratin) 0 stiffness 0 itch 0 tingling 0

As shown in Tables 11 and 12, after one application of the hair shampoo according to Example 1-1 of the present invention, the amount of sebum was improved by 80% or more compared to before, confirming that the scalp oil improvement effect was excellent, and these results are shown in Tables 13 to 15 It was consistent with the results of the questionnaire on the subjects shown in Fig. that more than 90% of the subjects answered that they had cleansing and sebum removal effects without side effects.

1 to 3 are photographs showing enlarged observation results before (a) application and (b) after one application of the hair shampoo according to Example 1-1 of the present invention for three of the test subjects, and the cleaning and sebum removal effects were was found to be excellent.

Experimental Example 6. Confirmation of improvement in scalp flora

When using the hair shampoo composition according to Example 1, the hair treatment composition according to Example 2, and the hair tonic composition according to Example 3, the effect of improving the scalp flora was confirmed. As the test subjects, 24 adult women with an average age of 45 were selected, and the test subject's scalp was selected as the test site. The test subject was prohibited from using other products such as shampoos, treatments, and hair essences, and from hair treatments such as dyeing and perms during this experiment.

After allowing the test subject to rest for 30 minutes in a constant temperature and humidity room at 20 to 25° C. and 40 to 60% humidity, the experiment was performed, and all experiments were performed in the constant temperature and humidity room. After the test subject sufficiently wets the hair and scalp with lukewarm water, the hair shampoo composition according to Examples 1-1 and 1-2 and the hair treatment composition according to Example 2 were applied to the hair and scalp in the same amount once a day, respectively. Apply evenly to the scalp and massage, then rinse thoroughly with running water. In addition, the subject evenly applied the same amount of the hair tonic composition according to Example 3 to the scalp once a day, massaged, and then dried without rinsing.

After the experiment period, without shampoo and water applied to the hair and scalp for one day, the hair and scalp were allowed to rest for 30 minutes in the constant temperature and humidity room. Therefore, it was compared and analyzed with the microorganisms collected before the experiment.

Hereinafter, statistical processing in this experiment was analyzed using SPSS. As a result of the analysis, it was judged that there was an improvement effect when the significance probability p<0.05 in the 95% confidence interval. For statistical analysis, paired samples t-test was used when parametric method was used, and Wilcoxon signed rank test was used when nonparametric method was used. Table 16 shows the results of analysis of variance (ANOVA) at the genus level before and after using the composition according to the example.

Genus p value before use 1 week after use 2 weeks after use Aspergillus p < 0.01 2.21 2.01 0.78 Fusarium p < 0.01 1.58 1.49 0.28 Penicillium p < 0.01 1.12 0.99 0.21 Cladosporium p < 0.01 1.03 0.98 0.19 Mucor p < 0.01 0.66 0.71 0.2 Alternaria p < 0.01 0.6 0.56 0.13 Kluyveromyces p < 0.01 0.66 0.54 0.06 Cliotcybe p < 0.01 0.6 0.57 0.032 Sarocladium p < 0.01 0.53 0.55 0

After 2 weeks of using the composition according to the example, it was confirmed that the flora of 9 genera present on the scalp was statistically significantly changed. As shown in Table 16, from 1 week after using the composition according to Examples 1 to 3 of the present invention, the genus Saccharomyces , known to contribute to scalp moisturizing and elasticity improvement, increased statistically significantly, Aspergillus, which is known to cause mycoses or dermatitis, In the genera Fusarium and Penicillum , there was a statistically significant decrease after 2 weeks of use, confirming that a statistically significant improvement of the flora was made on the scalp.

Experimental Example 7. Confirmation of improvement in skin flora

The skin flora improvement effect was confirmed when the body wash composition according to Example 4 and the body lotion composition according to Example 5 were used. As the test subjects, 22 adult women aged 20 to 60 years were selected, and the skin of the forearm of the test subject was selected as the test site. The test subject was prohibited from using other body products during this test period.

The test was performed after the test subject was allowed to rest for 30 minutes in a constant temperature and humidity room at 20 to 25° C. and 40 to 60% humidity without washing the test site for more than 8 hours, and all experiments were performed as described above. This was done in a constant temperature and humidity room. The test subject wet the skin of the forearm with lukewarm water, washed with the body wash composition according to Example 4, and evenly applied the body lotion composition according to Example 5.

After the experiment period, the microbes were collected by swabs up and down 40 times with a cotton swab on the skin of the forearm, and compared with the microbes collected before the experiment. Statistical processing was performed in the same manner as in Experimental Example 6 described above. Table 17 shows the results of analysis of variance (ANOVA) at the genus level before and after using the composition according to the example.

Genus p value before use 1 week after use 2 weeks after use Ehrlicia p < 0.001 7.43 7.54 2.62 Sphinomonas p < 0.001 3.19 3.22 2.29 Pseudomonas p < 0.001 1.56 1.5 3.31 Acetobacter p < 0.001 2.65 2.7 0.95 Cutibacterium p < 0.001 1.22 1.17 3.07 Streptococcus p < 0.001 1.13 0.96 1.93 Akkermansia p < 0.001 1.55 1.56 0.85 Bacteroides p < 0.001 1.37 1.41 0.69 Staphylococcus p < 0.001 0.72 0.72 1.81 Enterococcus p < 0.001 0.93 0.92 0.80

After 2 weeks of using the composition according to the example, it was confirmed that the flora of 10 genera present on the skin was statistically significantly changed. As shown in Table 17, Staphylococcus and Cutibacterium genus increased statistically significantly after 2 weeks of use of the compositions according to Examples 4 and 5 of the present invention, and Ehrlichia, Sphingomonas , etc., which are known to cause infections, were statistically significant after 2 weeks of use. Significantly decreased, it was confirmed that a statistically significant improvement of the flora in the skin was made. In addition, as a result of measuring the diversity of the flora (β-diversity), it was confirmed that a statistically significant increase was confirmed, and it was confirmed that a statistically significant improvement of the flora in the skin was made.

It goes without saying that the present invention is not limited to the embodiments described above, and a combination of the embodiments or a combination of at least one of the embodiments and a known technology may be included as another embodiment.

Although the present invention has been described in detail through specific examples, it is intended to describe the present invention in detail, and the present invention is not limited thereto, and by those of ordinary skill in the art within the technical spirit of the present invention It will be clear that the transformation or improvement is possible.

All simple modifications or changes of the present invention fall within the scope of the present invention, and the specific protection scope of the present invention will become apparent from the appended claims.

Claims (17)

It contains polysaccharides, yeast extracts, and fermented products of strains with probiotic properties as active ingredients,
The yeast extract is
A cosmetic composition for skin improvement, characterized in that it is a truffle-derived yeast extract. The method of claim 1,
The polysaccharide is
A cosmetic composition for skin improvement, characterized in that at least one selected from the group consisting of inulin, beta-glucan and maltodextrin. delete The method of claim 1,
The strain having the probiotic properties,
A cosmetic composition for skin improvement, characterized in that at least one selected from the group consisting of Lactobacillus sp. strain and Bifidobacterium sp. strain. 5. The method of claim 4,
The fermented product is a cosmetic composition for skin improvement, characterized in that at least one selected from the group consisting of a fermentation lysate and a fermentation filtrate. The method of claim 1,
Inulin, beta-glucan, maltodextrin, brewer's yeast extract, Lactobacillus fermentation lysate and Bifidobacterium fermentation lysate as active ingredients. 7. The method of claim 6,
A cosmetic composition for skin improvement, comprising inulin, beta-glucan, maltodextrin, brewer's yeast extract, truffle-derived yeast extract, Lactobacillus fermentation lysate and Bifidobacterium fermentation lysate, respectively, in the same weight ratio. 7. The method of claim 6,
Based on 100% by weight of the cosmetic composition, at least one active ingredient selected from the group consisting of inulin, beta-glucan, maltodextrin, brewer-derived yeast extract, truffle-derived yeast extract, Lactobacillus fermentation lysate and Bifidobacterium fermentation lysate A cosmetic composition for skin improvement, comprising 0.00001 to 10% by weight. The method of claim 1,
The cosmetic composition,
A cosmetic composition for improving skin, characterized in that it is for improving the microbial flora inhabiting the skin. 10. The method of claim 9,
The cosmetic composition,
A cosmetic composition for improving skin, characterized in that it promotes the growth of beneficial bacteria inhabiting the skin or inhibits the growth of harmful bacteria. 11. The method of claim 10,
The beneficial bacteria is, Staphylococcus epidermidis ( S. epidermidis ),
The harmful bacteria, Staphylococcus aureus ( S. aureus ) A cosmetic composition for skin improvement, characterized in that it is. The method of claim 1,
The cosmetic composition,
A cosmetic composition for skin improvement, characterized in that it is for skin soothing, skin wrinkle improvement, and skin elasticity improvement. The method of claim 1,
The composition includes lotion, facial lotion, body lotion, nourishing cream, moisture cream, eye cream, essence, cosmetic ointment, spray, gel, pack, sunscreen, makeup base, foundation, powder, cleansing cream, cleansing lotion, cleansing oil, A cosmetic composition for improving skin, characterized in that it is a cleansing foam, soap or body wash formulation. The method of claim 1,
The skin is a cosmetic composition for improving skin, characterized in that the scalp. 15. The method of claim 14,
The cosmetic composition,
A cosmetic composition for skin improvement, characterized in that it is any one for scalp soothing, scalp oil improvement, hair loss prevention, and hair growth improvement. 15. The method of claim 14,
The cosmetic composition,
A cosmetic composition for skin improvement, characterized in that it is for improving the microbial flora inhabiting the scalp. 15. The method of claim 14,
The skin is the scalp,
The composition includes hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair conditioner, hair treatment, hair cream, hair nutrition cream, hair moisture cream, hair massage cream, hair wax, hair aerosol, Hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservative, hair dye, hair wave agent, hair bleach, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer, hair A cosmetic composition for improving skin, characterized in that it is in the form of a mousse or hairspray.

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