TW200800289A

TW200800289A - Promoting agent for vitamin c transporter producing

Info

Publication number: TW200800289A
Application number: TW096105445A
Authority: TW
Inventors: Tomohiro Chiba; Kaori Aibe; Miho Shukuri; Tetsuhito Sakurai; Naoko Yamazaki; Takamasa Hitomi
Original assignee: Fancl Corp
Priority date: 2006-02-14
Filing date: 2007-02-14
Publication date: 2008-01-01
Also published as: CN101912352A; JP5571897B2; TWI389707B; JPWO2007094312A1; WO2007094312A1

Abstract

This invention provides a material having a vitamin C transporter producing promoting action. The vitamin C transporter producing promoting agent contains the compounds of marrubiin, cholesteryl benzoate, ugaferin, lapiferin, geranyl acetate, nerolidol, dihydrojasmone, carvacryl acetate, guaiazulene, dihydroconiferyl alcohol, stevioside, DL-α-pinene, verbenalin, 1-heptacosanol, 4- hydroxy coumarin, cholic acid, cholesteryl oleate, fraxin, bergaptol, or the extracts of Mognalia obovata, Prunus dulcis, Hydrangemacrophylla, Citrus paradise, Ophiopogon jponicus, Hedera heli, saponiria officinalis, Houttuynia cordata, Matricaria chamomilla, Uncalia gambir, Hamamelis virginiana, Morus alba, Oryza sativa(rice bran) as active ingredient(s).

Description

200800289 九、發明說明：【發明所屬之技術領域】 • 本發明係有關具有維生素c轉運體生產促進作用之化、w合物及植物。又，有關利用該化合物及植物之維生素C吸收k進用組成物、含有該維生素C吸收促進用組成物之美白用組成物或膠原合成促進用組成物。、【先前技術】、准生素C作為生體内水溶性抗氧化物質，對於超氧吟離子（卿⑽Xide aniQn)或其他活性氧具有優越之抗氧: 作用，可預防細胞内脂質成分之氧化。又，控制已知作為膠原合成中脯胺酸殘基之經化<里 … 酵素之㈣，可調整經由維生素合作用 Π有=調節氧化及還原，而參予種種酵素反應:雖為具有夕機成之維生素C，惟包括土撥鼠及類，由咖L切酸内醋氧化酶“一丨二長_ ’而無合成維生素c之能力，又，由於维生二心〜㈣# ’與其㈣雜維生素時間短’咸認^導至維生素c之機以足/、生虹保持齋二：，素c缺乏引起之疾病可列舉如壞血病、創傷户癒月匕力降低、骨或結缔組織障礙、又，對於心臌咗— g孑勁不文疋化等。 J方、、蜮疾病、癌症、白生素C亦可以預防式、… 早饮目寺種種疾病’維又，除了疾病以外=ΓΓ而使用（非專利文獻υ。膚之濕潤、抗老化竹對於肌膚亦提供多種以美白、強化肌柷老化作用為目的之維生素C製劑。 318986 6 200800289 另一方面，關於維生素c之機能或其有效性之見解雖有多數報告，惟，對於各組織吸收維生素c .多不明之點。於1999年，一 Μ等人從大鼠成:： ’殖擔任吸收維生素C之膜蛋白質，而發現以納離子：維生素C=2:l之比例向細胞内輸送之鈉依存性維生素體（SVCT)(非專利文獻2)。其後之研究亦明瞭在人類亦^ 在有維生素C運轉體，又明瞭維生素c運轉體^型⑼ 籲及2型（SVCT2)2種異構體。SVCT1主要分布於小腸、肝臟、腎臟、大腸、子宮、前列腺等上皮組織，跳丁2則廣泛分布於肺、骨格肌、眼、骨、腦等内皮組織。雖然均特異性地輸送維生素C，惟，經由速度論之解析可明瞭，在特異性方面SVCT2比SVCT1優越（非專利文獻3)。200800289 IX. DESCRIPTION OF THE INVENTION: TECHNICAL FIELD OF THE INVENTION The present invention relates to a compound having a vitamin c transporter production promoting action, a w compound, and a plant. In addition, the vitamin C-absorbing composition using the compound and the plant, and the whitening composition or the collagen synthesis-promoting composition containing the vitamin C absorption-promoting composition. [Previous technique] As a water-soluble antioxidant substance in the body, the biotin C has superior antioxidant effect on superoxide cesium ions (Q(X) ideQn) or other reactive oxygen species, and can prevent oxidation of intracellular lipid components. . In addition, it is known that it is known as the chemical reaction of the proline residue in collagen synthesis. (IV) The enzyme can be adjusted to regulate oxidation and reduction via vitamin cooperation, and various enzyme reactions are involved: The vitamin C of the machine, including the woodchuck and the class, is the ability of the vinegar oxidizing enzyme to cut the acid vinegar oxidase "one 丨 two long _ ' without synthetic vitamin C, and, because of the life of the two hearts ~ (four) # ' (4) Short time for miscellaneous vitamins 'salt to the vitamin c machine to the foot /, Shenghong to keep the fast two:, the disease caused by the lack of c can be listed as scurvy, traumatic dysfunction, bone or knot Organizational barriers, and, for the heart, g孑, 不不。。 J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J J It is used outside of the disease = non-patent literature. The moisturizing and anti-aging bamboo of the skin also provides a variety of vitamin C preparations for whitening and strengthening the tendon aging effect. 318986 6 200800289 On the other hand, about vitamin c Although there are many opinions on function or its effectiveness Several reports, however, are unclear about the absorption of vitamin C by various organizations. In 1999, a scorpion and other people from the rat into:: 'reported to absorb the membrane protein of vitamin C, and found in nano-ion: vitamin C = A sodium-dependent vitamin body (SVCT) delivered to the cells in a ratio of 2:1 (Non-Patent Document 2). Subsequent studies have also revealed that in humans, there are vitamin C-operating bodies, and vitamin C-operating bodies are also known. (9) Call for type 2 (SVCT2) two isomers. SVCT1 is mainly distributed in epithelial tissues such as small intestine, liver, kidney, large intestine, uterus, prostate, etc., and mitral 2 is widely distributed in lung, bone muscle, eye, bone, brain. Although the endothelial tissue is specifically transported, it is clear from the analysis of the speed theory that SVCT2 is superior to SVCT1 in terms of specificity (Non-Patent Document 3).

Alexander等人對於隨著年齡增加，維生素c保有量與維生素C轉運體表現量之關係進行研究，而報告指出在老鼠肝臟中，維生素C轉運體（SVCT1)之表現量降低及維生素c保有量減少（非專利文獻4)。預防或改善維生素c保有量降低之方法，可列舉連續攝取維生素c，惟，MacD〇naid 等人使甩人類腸管模型推測攝取過量之維生素c，則維生素C轉運體（SVCT1)之表現降低，維生素c之吸收能力降低 (非專利文獻5)。從該等見解認為在有關預防或改善因維生素C保有量降低而引起之維生素c機能降低方面，維生素C轉運體生產促進劑為有效之方法。至今關於提高SVCT1及SVCT2之表現之化合物或植物之報告極少。FukiU等人報告使用小鼠骨芽細胞，由於得 318986 7 200800289 撒米松（Dexamethasone)提高SVCT2之表現，而提昇維生素 C之吸收量（非專利文獻6)。惟，由於得撒米松顯示荷爾蒙作用，從副作用之觀點而言，在使用上受到極大之限制。 v又’對於增強SVCT1表現之化合物或植物方面並無相關之報告。非專利文獻 1 : Molecular Membrane Biology，2001， v〇1.1 85 87-95 _ 非專利文獻 2 : Nature，1999, VoL 399, 70-75 非專利文獻 3 ·· FEBS Letter，1999, Vo 1. 460, 480-484 非專利文獻 4 : Archives of Biochemistry and Biophysics, 20035 V〇L 4105 112-120 非專利文獻5 Vol·87, 97-100 非專利文獻6 Vol·86, 145-149 【發明内容】Alexander et al. studied the relationship between vitamin C retention and vitamin C transporter expression with increasing age, and reported that in the liver of mice, the amount of vitamin C transporter (SVCT1) decreased and vitamin C retention decreased. (Non-Patent Document 4). The method of preventing or improving the reduction of the amount of vitamin C can be cited as continuous intake of vitamin C. However, if MacD〇naid et al. make the human intestinal tube model speculate that excessive intake of vitamin C, the performance of vitamin C transporter (SVCT1) is lowered, vitamins The absorption capacity of c is lowered (Non-Patent Document 5). From these findings, it is considered that a vitamin C transporter production promoter is an effective method for preventing or improving the vitamin C function caused by a decrease in the amount of vitamin C. Reports on compounds or plants that have improved the performance of SVCT1 and SVCT2 to date have been rare. FukiU et al. reported the use of mouse bone bud cells to increase the absorption of vitamin C by increasing the performance of SVCT2 by Dexamethasone (Non-patent Document 6). However, because of the hormonal effect of dexamethasone, it is extremely limited in its use from the point of view of side effects. There are no reports of compounds or plants that enhance the performance of SVCT1. Non-Patent Document 1: Molecular Membrane Biology, 2001, v〇1.1 85 87-95 _ Non-Patent Document 2: Nature, 1999, VoL 399, 70-75 Non-Patent Document 3 · FEBS Letter, 1999, Vo 1. 460, 480-484 Non-Patent Document 4: Archives of Biochemistry and Biophysics, 20035 V〇L 4105 112-120 Non-Patent Document 5 Vol. 87, 97-100 Non-Patent Document 6 Vol. 86, 145-149 [Summary of the Invention]

British Journal of Nutrition, 2002,British Journal of Nutrition, 2002,

British Journal of Nutrition，2001， [發明欲解決之課題] 本發明之課通在提供促進維生素C在標的組織吸收之、准生素C轉運體生產促進劑。本發明之另一課題為提供含有該等促進劑之美白用組成物或膠原合成促進用組成物。 [解決課題之方法] 本發明人等為了解決上述課題進行深入研究結果發現了有、’隹生素c轉運體生產促進作用之化合物及植物等，作為維生素C吸收促進用組成物、美白用組成物、膠原合成 318986 8 200800289 促進用組成物非常有用’ 案之解決方法係如下述。1成本备明。亦即，此處提 (1)以含有至少—種以上選 + 、醋、幽卡菲林、拉皮菲林、夭夏早古素、膽巢基苯甲酸氫茉莉酮、香芹_ 曰茱基乙酸酯、橙花叔醇、二 4日斤基乙酸酯、侖瘃盆 ^ 讪-a-菠烯、馬鞭草苦大、二虱松柏醇、甜菊糖、膽酸、膽固醇油酸g| ;十：：醇、基香豆素、特=維生素c轉運體生產促進:手齡所成之化合物群為物載之任何—種化合物之植物、動物、炉物、微生物之粉碎物、其萃取動勿礦維生素c轉運體生產促進劑。、有卒取物之水解物之 (3)由上述（1)或（2)記载之 & 維生素c或維生以行生抓/人運肢生產促進劑、及用組成物。素^生物組合而成之維生W促進 (4:維生素C知生物為2_〇_ 〇葡萄吼喃糖基 Ulucopyranosyl)-抗壞血收促進用組成物·&之上述⑶記載之維生素4 述⑶或⑷記敎维生以㈣促^組成物之吴白用組成物。 (’上述（3)或（4)δ&載之維生素C吸收促進用組成物之爆、原合成促進用組成物。 (jo以含有至少-独上選自由厚朴、純仁、繡球花、葡苟柚、龍鬚草、西洋常春藤、石驗草、魚腥草、洋甘菊、兒余、金縷梅、桑、米糖、問莉所成組群之粉碎m 318986 9 200800289 c轉運體生產取物或含有萃取物之水解物為特徵之促進劑。 ^ (8)以由上述（7)記載之維生素c轉峰夸Γ々输L 士 ^ 生產促進劑、及維生素C或維生素c衍生物組合而成促進用組絲。紅料素卜及收 (9)維生素c衍生物為2-〇—a —J)—-气 ^ u 9甸吡喃瑭基-抗壞血酸之上述⑻記载之維生素卜及收促進用組成物。British Journal of Nutrition, 2001, [Problems to be Solved by the Invention] The present invention provides a promoter for the production of a vitamin C transporter which promotes the absorption of vitamin C in a target tissue. Another object of the present invention is to provide a whitening composition or a collagen synthesis-promoting composition containing the accelerator. [Means for Solving the Problem] In order to solve the above problems, the inventors of the present invention have found that a compound having a promotion effect of the production of a vitamin C transporter, a plant, and the like, and a composition for promoting vitamin C absorption and whitening Material, collagen synthesis 318986 8 200800289 Promoting the composition is very useful' The solution is as follows. 1 cost is stated. That is, here (1) to contain at least one of the above selection +, vinegar, cecaline, riviphenanthine, sylvestre, jasmine benzoyl benzoate, celery _ 曰茱乙乙Acid ester, nerolidol, bismuth acetate, 瘃瘃 pot 讪-a-spinene, verbena bitter, diterpenoid, stevioside, cholic acid, cholesterol oleic acid g| 10: Alcohol, coumarin, special = vitamin c transporter production promotion: the compound group formed by the age is the plant, animal, stove, microbial pulverized material of any compound, and its extraction Do not mine vitamin c transporter production promoter. (3) The vitamin C or vitamins described in (1) or (2) above, the biogravity/human limb production accelerator, and the composition. The vitamin W is promoted by the combination of the genus and the genus (4: vitamin C is known as the genus 2 〇〇〇吼 U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U U (3) or (4) Record the composition of Wu Bai for the composition of (4). ('The above-mentioned (3) or (4) δ & the composition for promoting the promotion of the vitamin C absorption-promoting composition, and the composition for promoting the original synthesis. (jo contains at least - alone selected from Magnolia, Pure Kernel, Hydrangea, Portuguese苟苟苟龙龙 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 318 An agent or a hydrolysate containing an extract is a promoter. ^ (8) A vitamin C-transformed product of the above-mentioned (7) is used to produce a promoter, and a vitamin C or vitamin C derivative combination. It is used to promote the use of silk. The red material and the (9) vitamin C derivative are 2-〇-a-J)--qi^u 9-dipyridinyl-ascorbic acid. And the composition for promotion.

〇〇)以含有上述（8)或（9)記載之維生辛c吸收彳物為特徵之美白用組成物。素。及收促進用組成含有上述⑻或⑼記載之維生素c吸收促進用組成物為知徵之膠原合成促進用组成物。 (12) 以含有上述(3)、⑷、⑻或(9)記載之維生素c吸收促U組成物及為特徵之美自用組成物。 (13) 葡糖苷酶劑為至少一種以上選自紫錐花 (Echinacea)酵母、雙叉桿菌⑹、乳酸菌所成組群之粉碎物、其萃取物或萃取水解物之上述（白用組成物。秀 [發明效果] 只要含有本發明發現之特定化合物及植物之維生素c 轉運體生產促㈣，在使用細胞之解析中，可確認顯著促進SVCT1及/或2之_Α表現。本發明之維生素c轉運體生產促進劑可湘作為美自肋成物、縣合成促進用組成物’其使用形態列可舉σ服用、皮膚外用、注射、化輕品、鲁樂品等0 3]8986 10 200800289 【實施方式】 [實施本發明之最佳形態] • 以下，對本發明加以詳細說明。本發明之維生素c轉 v運體生產促進劑中所含之化合物可使用夏至草苦素、膽巢基苯曱酸醋、幽卡菲林、拉皮菲林、香葉基乙酸酯、橙花叔醇、二氫茉莉酮、香芹基乙酸酯、愈瘡奠、二氫松柏醇、甜菊糖、DL—α〜蒎烯、馬鞭草苷、1-二十七烷醇、4_羥基 ❿香豆素、膽酸、膽固醇油酸酯、椁皮苷、佛手酚等。該等化合物可單獨使用1種，亦可將2種以上組合使用。又，亦可使用含有該等化合物之植物、動物、礦物、微生物之粉碎物、其萃取物或萃取水解物。含有夏至草苦素（CAS:465-92-9)之植物可列舉唇形科夏至草屬之杯狀夏至草（Marrubium acetabulosum)、阿德为夏至旱（Marrubiinn adfine)、阿連夏至草（Marrubiuni aelleni i)、阿芬夏至草（Marrubium affine)、非洲夏至草 ⑩（Marrubium africanum)、阿普夏至草（Marrubiiim album)、阿德尼殿夏至草（Marrubium al ternidens)、阿索德斯夏至草（Marrubium alyssoides)、庭芥夏至草（Marrubium alysson)、狹葉夏至草（Marrubium angusti fol ium)、阿尼松楝夏至草（Marrubium anisodon)、阿普倫夏至草 (Marrubium apulum)、水生夏至草（Marrubium aquaticum)、阿謝松夏至草（Marrubium aschersoni)、阿斯特卡尼坤夏至草（Marrubium astracanicum)、大西洋夏至草（Marrubium atlanticum)、阿雅德夏至草（Marrubium 11 318986 200800289 ayardii)、巴洛夏至草（Marrubiumballota)、巴洛塔奉夏至草（Marrubiiim bal lotaef ooie)、巴洛特斯夏至草 ¥ (Marrubiumballotoides)、巴斯特塔奴夏至草（Marrubium vbastetanum)、波姆勒夏至草（Marrubiuro bornmuelleri)、柏蓋夏至草（Marrubium bourgaei)、普拉丘棟夏至草 (Marrubium brachyodon)、純白夏至草（Marrubium candidssimum)、卡拉利菲歐林夏至草(Marrubium 馨 catariaefolium)、頭花夏至草（Marrubium cephalanthum)、席聶拉線夏至草（Marrubium cinerascens)、灰白夏至草（Marrubium cinereum)、渦捲夏至草（Marrubium circinnatum)、喜畢斯夏至草 (Marrubium civice)、藍青夏至草（Marrubium coerulescens)、密夏至草（Marrubium condensatum)、心形葉夏至草（Marrubium cordatum)、克拉席殿斯夏至草 (Marrubium crassidens)、地中海夏至草（Marrubium _ creticura)、縐曲夏至草（Marrubium crispum)、楔葉夏至草（Marrubium cuneatum)、席列奴夏至草（Marrubium cy 11 eneum)、盆弱夏至草（Marrubium depauperatum)、荒地夏至草（Marrubium desrti)、丟阿辨斯夏至草 (Marrubium duabense)、芒刺夏至草（Marrubium echinaturn)、軟毛夏至草（Marrubium eriocephalum)、軟穗夏至草（Marrubium eriostachyum)、法席澱斯夏至草 (Marrubium faucidens)、黃夏至草（Marrubiiim flavum)、波狀夏至草（Marrubium flexuosum)、湧泉地生夏至草 12 318986 200800289 (Manrubium fontianum)、福來瓦德斯卡奴夏至草 (Marrubium f riwaldskyanum)、卡模楝夏至草（Marrubi .um gamodon)、德國夏至草（Marrubium germanicum)、葛雷 v可麥佛利姆夏至草（Marrubium glechomaefol ium)、球狀夏至草（Marrubium globosum)、鉤克斯查坤夏至草 (Marrubium goktschaicum)、蓋利魯麥夢夏至草 (Marrubium gull iermondii)、鉤狀夏至草（Marrubium 馨hamatum)、赫莫尼斯夏至草（Marrubium hermonis)、異枝夏至草（Marrubium heterocladum)、異齒夏至草 (Marrubium heterodon)、耶拉普利塔姆夏至草（Marrubium hierapolitanum)、粗毛夏至草（Marrubium hirsutum)、西班牙夏至草（Marrubium hi spanicum)、阿姆伯特夏至草 (Marrubium humbertii)、海波肯夏至草（Marrubium hyperleucum)披白毛夏至草（Marrubium incanum)、風車草夏至草（Marrubium incisum)、魚針草夏至草（Marrubium 馨 indicum)、可斯基夏至草（Marrubium kotschyi)、卡迪坤夏至草（Marrubium kurdicum)、庫斯聶若夏至草 (Marrubium kusnezowii)、拉蜜歐迪斯夏至草（Marrubium 1 amioides)、軟毛夏至草（Marrubium lanatum)、似落葉松屬夏至草（Marrubium laricum)、似落葉松葉夏至草 (Marrubium laur i f 〇1 ium)、雷歐奴若依迪夏至草 (Marrubium leonuroides)、利巴諾娣坤夏至草（Marrubium libanoticura)、利塔迪銳夏至草（Marrubium 1 itardierei )、淡黃夏至草（Marrubium lutescens)、瑪克 13 318986 200800289 洛楝夏至草（Marrubium macrodon)、瑪克洛密阿奴夏至草 (Marrubium malcolmianum)、瑪魯畢夏至草（Marrubium .marrubiastrum)、小花夏至草（Marrubium micranthum)、 •小葉夏至草（Marrubium microphy 11 um)、細毛夏至草 (Marrubium mollissimum)、夢特妹格利奴夏至草 (Marrubium montemegrinum)、多葱夏至草（Marrubium multi bract eatum)、矮種夏至草（Marrubium nanum)、黑夏 ⑩至草（Marrubium nigrum)、諾耶奴夏至草（Marrubium noeanum)、香水夏至草（Marrubium odoratissimum)、東洋夏至草（Marrubium orientale)、淡白夏至草（Marrubium pallidum)、錐狀花夏至草（Marrubium paniculatum)、潘諾妮坤夏至草（Marrubium pannonicum)、帕娜席坤夏至草 (Marrubium parnassicum)、小花夏至草（Marrubium parvi f lorum)、疏花夏至草（Marrubium pauci f lorum)、外來夏至草（Marrubium peregrinum)、伊朗夏至草 ⑩ (Marrubium persicum)、培斯他洛雜夏至草（Marrubium pestalozzae)、疊重夏至草（arrubium pi icatum)、羽狀夏至草（Marrubium plumosum)、革葉夏至草（Marrubium polyodon)、早生夏至草（]^〇*1113111111口^6(：(^)、高種夏至草（Marrubium procerum)、似夏至草（Marrubium propinquum)、偽庭芬夏至草（Marrubium pseudo-alyssum)、偽白鮮夏至草（Marrubium pseudo-dictamnus)、紫夏至草（Marrubium purpureum)、放射狀夏至草（Marrubium radiatum)、疏著夏至草（Marrubium 14 318986 200800289 remotum)、圓葉夏至草（Marrubium rotundifol ium)、赤夏至草（Marrubium rubrum)、皺紋夏至草（Marrubium .rugosum)、玄蔘葉夏至草（Marrubium _ scrophulariaef ol ium)、協鉤伯利線夏至草（Marrubium segobricense)、協利協坤夏至草（Marrubium sericeum)、刺狀夏至草（Marrubium setaceum)、協威走夏至草 (Marrubium sewerzowi)、亞低木夏至草（Marrubium 馨 suff rut icosum)、擴張夏至草（Marrubium supinum)、協莎魯姆夏至草（Marrubium thessalum)、陶依妮夏至草 (Marrubium thouini)、特拉奇提坤夏至草（Marrubium trachyticura)、特窺畢克夏至草（Marrubium turkeviczi i)、鉤狀夏至草（Marrubium uncinatum)、波狀夏至草（Marrubium undulatum)、拜欄特夏至草（Marrubium vail lanti i)、巴念斯夏至草（Marrubium vanense)、天鵝織夏至草（Marrubium velutinum)、淡綠夏至草（Marrubium ⑩ virescens)、代肯尼夏至草（Marrubium vulcanicum)、夏至草（Marrubium vulgare)、貝魯聶夏至草（Marrubium werneri )、威可米夏至草（Marrubium wi lk⑽mi)、I 形科益母草屬之益母草（Leonurus cardiaca)、唇形科獅耳花屬之獅耳花（Leonotis leonurus)等。含苯甲酸膽固醇酯（CAS: 604-32-0)的動物可列舉海龍科之尖海龍（Syngnathus acus)。含幽卡菲林（CAS: 63026-58-4)之植物可列舉傘形科阿魏屬之阿利釣妮阿魏（Ferula arrigoni i )、阿魏草（Ferula 15 318986 200800289 assafoetida)、大茴香（Ferula communis)、總苞阿魏 (Ferula involucrata)、庫西施塔妮卡阿魏（Ferula λ kuhistanica)、多石阿魏（Ferula lapidosa)、廣羽片阿魏 (Ferula latipinna) ' 白穗阿魏（Ferula leucographa)、〇〇) A composition for whitening which is characterized by containing the vitamin C absorption enthalpy described in (8) or (9) above. Prime. The composition for promoting the promotion of the vitamin C containing the composition for promoting vitamin C absorption as described in the above (8) or (9) is a composition for promoting collagen synthesis. (12) A vitamin C-absorbing U composition as described in the above (3), (4), (8) or (9) and a composition for beauty use. (13) The glucosidase agent is at least one of the above-mentioned (white composition) selected from the group consisting of Echinacea yeast, Bifidobacterium (6), a pulverized product of a group of lactic acid bacteria, an extract thereof or an extract hydrolyzate. [Effect of the Invention] As long as the production of the vitamin c transporter of the specific compound and the plant found in the present invention is promoted (4), it is confirmed that the expression of the used cells significantly promotes the expression of SVCT1 and/or 2. The transporter production promoter can be used as a composition for promoting the synthesis of the ribs and the county, and its use forms include sputum taking, skin external application, injection, chemical light products, Lule products, etc. 3 3] 8986 10 200800289 [ BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below. The compound contained in the vitamin c-trans transport product production promoter of the present invention can be used as a compound of summer solstice and benzoyl benzoquinone. Sour vinegar, acesulfameline, lapifenline, geranyl acetate, nerolidol, dihydrojasmone, carvyl acetate, guaiac, dihydroconiferol, stevioside, DL-α ~terpene, verbin 1 - hexadecanol, 4 - hydroxy coumarin, cholic acid, cholesterol oleate, quercetin, bergamot, etc. These compounds may be used alone or in combination of two or more. Further, a plant, an animal, a mineral, a microbial pulverized product, an extract thereof or an extract hydrolyzate containing the same may be used. A plant containing a summer solani (CAS: 465-92-9) may be exemplified by a lip family. Marrubium acetabulosum, Amaranthus marrubiinn adfine, Marrubiuni aelleni i, Marrubium affine, Marrubium africanum , Marrubiiim album, Marrubium al ternidens, Marrubium alyssoides, Marrubium alysson, Marrubium angusti fol Ium), Marrubium anisodon, Marrubium apulum, Marrubium aquaticum, Marrubium aschersoni, Aspen Marrubium astracanicum, Marrubium atlanticum, Marrubium 11 318986 200800289 ayardii, Marrubiumballota, Marrubiiim bal lotaef ooie ), Marrubiumballotoides, Marrubium vbastetanum, Marrubiuro bornmuelleri, Marrubium bourgaei, Purachus summer solstice (Marrubium brachyodon), Marrubium candidssimum, Marrubium catariaefolium, Marrubium cephalanthum, Marrubium cinerascens, gray summer sorghum Marrubium cinereum), Marrubium circinnatum, Marrubium civice, Marrubium coerulescens, Marrubium condensatum, Marrubium cordatum, Marrubium crassidens, Mediterranean summer Grass (Marrubium _ creticura), Marrubium crispum, Marrubium cuneatum, Marrubium cy 11 eneum, Marrubium depauperatum, and summer sorghum grass Marrubium desrti), Marrubium duabense, Marrubium echinaturn, Marrubium eriocephalum, Marrubium eriostachyum, Marrubium faucidens ), Marrubiiim flavum, Marrubium flexuosum, spring summer grass 12 318986 200800289 (Manrubium fontianum), Marabuium f riwaldskyanum, card model Marrubi .um gamodon, Marrubium germanicum, Marrubium glechomaefol ium, Marrubium globosum, Crochet Chauncay (Marrubium goktschaicum), Marrubium gull iermondii, hooked summer solstice (Marrubium xin ha) Matum), Marrubium hermonis, Marrubium heterocladum, Marrubium heterodon, Marrubium hierapolitanum, Marrubium hirsutum ), Marrubium hi spanicum, Marrubium humbertii, Marrubium hyperleucum, Marrubium incanum, Marrubium incisum, Sabina chinensis (Marrubium indicum), Marrubium kotschyi, Marrubium kurdicum, Marrubium kusnezowii, La Meridian summer solstice Marrubium 1 amioides), Marrubium lanatum, Marrubium laricum, Marrubium laur if 〇1 ium, Marrubium leonuroides, Marrubium libanoticura, Marrubium 1 itardierei, yellowish Marrubium lutescens, Marque 13 318986 200800289 Marrubium macrodon, Marrubium malcolmianum, Marrubium.marrubiastrum, Marrubium Micranthum), • Marrubium microphys 11 um, Marrubium mollissimum, Marrubium montemegrinum, Marrubium multi bract eatum, dwarf summer solstice (Marrubium nanum), Marrubium nigrum, Marrubium noeanum, Marrubium odoratissimum, Marrubium orientale, Marrubium pallidum, cone Marrubium paniculatum, Marrubium pannonicum, Marrubium parnassicum, Marrubium parvi f lorum, Marrubium pauci f lorum ), exotic summer solstice (Marrubium peregrinum), Iranian summer solstice 10 (Marrubium pers) Icum), Marrubium pestalozzae, arrubium pi icatum, Marrubium plumosum, Marrubium polyodon, early summer sorghum *1113111111 口^6(:(^), high-quality Marrubium procerum, Marrubium propinquum, Marrubium pseudo-alyssum, Marrubium pseudo-dictamnus ), Marrubium purpureum, Marrubium radiatum, Marrubium 14 318986 200800289 remotum, Marrubium rotundifol ium, Marrubium rubrum, wrinkles Marrubium rugosum, Marrubium _ scrophulariaef ol ium, Marrubium segobricense, Marrubium sericeum, Marrubium Setaceum), Xiawei, Marrubium sewerzowi, Marrubium suff rut icosum, Marrubium supi Num), Marrubium thessalum, Marrubium thouini, Marrubium trachyticura, Marrubium turkeviczi i, hooked summer solstice (Marrubium uncinatum), Marrubium undulatum, Marrubium vail lanti i, Marrubium vanense, Marrubium velutinum, light green summer solstice Marrubium 10 virescens), Marrubium vulcanicum, Marrubium vulgare, Marrubium werneri, Marrubium wi lk (10) mi, and motherwort of the genus I. (Leonurus cardiaca), the lion's ear flower (Leonotis leonurus). Animals containing cholesteryl benzoate (CAS: 604-32-0) may be referred to as Syngnathus acus. Plants containing sylvestre (CAS: 63026-58-4) can be found in the genus Ferula arrigoni i, ferula (Ferula 15 318986 200800289 assafoetida), and fennel (Ferula) Communis), Ferula involucrata, Ferula λ kuhistanica, Ferula lapidosa, Ferula latipinna 'Ferula Leucographa),

M 短葉阿魏（Ferula linkii)、席娜依卡阿魏（Ferula sinaica)、孫卡席亞阿魏（Ferula soongarcia)、帖奴依斯塔阿魏（Ferula tenuiesta)、烏崗姆阿魏（Ferula ugamica) •等。含有拉皮菲林（CAS: 86992-41-8)之植物可列舉傘形科阿魏屬之阿利鉤妮阿魏（Ferula arrigoni i )、阿魏草 (Ferula assafoetida)、大茴香（Ferula communis)、總苞阿魏（Ferula involucrata)、庫西施塔妮卡阿魏（Ferula kuhi stanica)、多石阿魏（Ferula lapidosa)、廣羽片阿魏 (Ferula 1 at i pinna)"白穗阿魏（Ferula leucographa)、短葉阿魏（Ferula l inki i )、席娜依卡阿魏（Ferula Φ sinaica)、孫卡席亞阿魏（Ferula soongarcia)、帖奴依斯塔阿魏（Ferula tenuiesta)、烏崗姆阿魏（Ferula ugamica) 等。含有香葉基乙酸酯（CAS: 105-87_3)之植物可列舉松科冷杉屬之大白葉冷杉（Abies mariesii)、菊科藿香薊屬之紫花藿香薊（Ageratum houstonianum)、菊科蒼朮屬之蒼朮 (Atractylodes lancea)、菊科酒神菊樹屬之酒神菊樹 (Baccharis dracuncul i fol 1 ia)、廣葉酒神菊樹 (Baccharis latifol ia)、柳葉酒神菊樹（Baccharis 16 318986 200800289 salicifollia)、菊科雛菊屬之雛菊（Beilis perennis)、菊科金盞花屬之金盞花（Calendula of ficinal is)、傘形科 ,歐芹屬之葛縷子（Carum carvi)、蕨藻科蕨藻屬之總狀蕨藻 _ (Caulerpa racemosa)、茄科夜香木屬之夜香木（Cestrum nocturnum)、傘形科明黨參屬之明黨參（Changium smyrnioides)、柑橘科柑橘屬之白檸檬（Citrus auranti fol ia)、酸橙（Citrus aurantium)、佛手柑（Citrus _ bergamia)、柚（Citrus grandis)、八朔柑（Citrus hassaku)、伊予抽（Citrus iyo)、香撥（Citrus junos)、乳橘（Ci trus kinokuni )、檸檬（Ci trus 1 imon)、圓佛手掛 (Citrus medica)、夏橙（Citrus natsudaidai)、葡萄柚 (Citrus paradise)、桎柑（Citrus reticulate)、甜橙 (Citrus sinensis)、小型柚（大分特產）（Citrus sphaerocarpa)、酸桔（Citrus sudachi)、三寶柑（Citrus sulcata)、橘柑（Citrus tachibana)、溫州橘（Citrus • unshiu)、松藻科松藻屬之紅藻（Codium tomentosum)、傘形科孜然芹屬之孜然芹（Cuminum cyminum)、禾本科香茅屬之青香茅（Cymbopogon caesius)、檸檬草（Cymbopogon ci trains)、卡西香茅（Cymbopogon khasi anus)、香水茅 (Cymbopogon winterianus)、傘形科胡蘿蔔屬之胡蘿蔔 (Daucus Carota)、唇形科青蘭屬之龍頭花（Dracocephalum kotschyi)、桃金娘科桉屬之垂尾桉（Eucalyptus urophylla)、菊科蠟菊屬之麥稈菊（Helichrysuin bracteatum)、魚腥草科魚腥草屬之魚腥草（Houttuynia 17 3189S6 200800289 cordata)、八角科八角屬之西藏八角（Η 1 i cium griffithii)、滇西八角（Illicium merrillianum)、柏科 ▲油杉屬之刺柏（Jimiperus f〇rmosana)、杜松（juniperus 、rigida)、桃金娘科松紅梅屬之爪哇細子（1^?1:〇3?6〇1_ javanicum)、傘形科女貞屬之女貞（Ligusticum mutellina)、馬鞭草科香水屬之爪哇馬鞭草（Lippia javanica)、唇形科夏至草屬之夏至草（Marrubium 着vulgare)、唇形科牛至屬之圓葉牛至（Origanum rotundif〇1 ium)、槐牛兒科天竺葵屬之香葉天竺葵 (Pelargonium graveolens)、樟科楠屬之巴西楠木（Ph〇ebe porosa)、杜鵑花科木鵑花屬之櫻草杜鑛（Rhododendron primulaef lorum)、薔薇科薔薇屬之五月玫瑰（Rosa centifolia)、唇形科鼠尾草屬之鼠尾草（Salvia clevelandi i)、低木鼠尾草（Salvia fructicosa)、鼠尾草 (Salvia officinalis)、香紫蘇（Salvia sclarea)、菊科 Φ 萬壽菊屬之萬壽菊（Tagetes erecta)、史君子科之安息香 (Terminalia bentzoe)、柏科崖柏屬之朝鮮崖柏（Thuja koraiensis)、唇科百里香屬之紅花百里香（Thymus sipyleus)、薰衣草百里香（Thymus thracicus)、法國百里香（Thymus vulgaris)、西班牙百里香（Thymus zygis)、柑橘科花椒屬之花椒（Zanthoxylum bungeanum)、翼柄花椒 (Zanthoxylum schinifolium)等。含有橙花叔醇（CAS : 7212-44-4)之植物可列舉菊科高山耆屬之咼山蓍草（Achi 1 lea alpine)、鳳尾（Achi 1 lea 18 318986 200800289 filipendulina)、利古里亞蓍草（AchiHea ligustica)、歐蓍草（Achillea mi lie i folium)、鷹香蓍草（Achillea ▲ moschata)、珠蓍（Achi 1 lea ptarmica)、黃金蓍（Achi 1 lea ¥ tomentosa)、菊科藿香薊屬之藿香薊（Ageratuin conyzoides)、紫花藿香薊（Ageratum houstonianum)、薑科莖蔻屬之香味菫蔻（Amomum aromaticum)、白菫蔻 (Am⑽um cardam⑽um)、香莖蔻（Amomum subulatum)、砂仁 • (Amomum xanthioides)、薑（Amomum zingiber)、豆科紫穗槐屬之紫穗槐（Amorpha fruticosa)、山茶科山茶屬之茶樹 (Camell ia sinensis)、豆科刀豆屬之白鳳豆（Canavalia ensiformis)、刀豆（Canavalia gladiata)、海刀豆 (Canaval ia maritima)、茄科夜香木屬之黃花夜香木 (Cestrum aurant iacum)、夜香木（Cestrum nocturnum)、紫花夜丁香（Cestrum purpureum)、柑橘科柑橋屬之白檸檬 (Citrus auranti fol ia)、酸橙（Citrus aurantium)、佛手 Φ 柑（Citrus bergamia)、柚（Citrus grandis)、八朔甜 (Citrus hassaku)、伊予柚（Citrus iyo)、香橙（Citrus junos)、(Citrus kinokuni) ' ##(Citrus limon)、圓佛手甜（Citrus medica)、夏橙（Citrus natsudaidai)、葡萄柚（Citrus paradise)、椏柑（Citrus reticulate)、甜橙（Citrus sinensis)、小型柚（大分特產）（Citrus sphaerocarpa)、酸桔（Citrus sudachi)、三寶柑（Citrus sulcata)、橘柑（Citrus tachibana)、溫州橘（Citrus unshiu)、大戟科巴豆屬之拗布雷畢雷巴豆（Croton 19 318986 200800289 aubrevi 1 lei)、古香皮木（Croton el uteri a)、日本野桐 (Croton japonicum)、烏臼木（Croton sebiferus)、巴豆，（Croton tigl ium)、渔姆貝席庫斯巴豆（Croton zambesicus)、傘形科胡蘿蔔屬之胡蘿蔔（Daucus carota)、木棉科權鏈屬之權楗（Durio zibethinus)、唇形科香薷屬之香薷（Elsholtzia ci 1 iata)、野拔子 (Elsholtzia rugulosa)、無患子科龍眼屬之龍眼鲁（Euphoria longan)、銀杏科銀杏屬之銀杏（Ginkgo bi loba)、柏科刺柏屬之杜松子（Juniperus communis)、洋杜松（Juniperus conferta)、刺柏（Juniperus formosana)、杜松（Juniperus rigida)、傘形科女貞屬之女貞（Ligusticum mutellina)、馬鞭草科過江藤屬之百棘枝（Lippia alba)、橙香馬鞭草（Lippia ci triodora)、爪哇馬鞭草（Lippia javanica)、過江藤（Lippia nodi f 1 ora)、馬魯佛洛拉馬鞭草（Lippia mal tuf 1 ora)、傘 ❿形科薄荷屬之水薄荷（Mentha aquatica)、胡椒薄荷 (Mentha arvensis)、薄荷（Mentha canadensis)、歐薄荷 (Mentha longi folia)、辣薄荷（Mentha piperita)、唇萼薄荷（Menthapulegium)、留蘭香（Mentha spicata)、傘形科畺味草屬之卡蜜聶亞之（Micromeria carminea)、山欖科香欖屬之香欖（牛油果）（Mi mu sops e 1 engi)、傘形科香草羅勒屬之羅勒（Ocimum basil icum)、巴西香草羅勒（〇cimum kilimandoscharicum)、細花羅勒（〇ciniuni tenuiflorum)、傘开》科傘形屬之水芹（Oenanthe javanica)、露兜樹科露兜 20 338986 200800289 樹屬之小笠原露兜樹（Pandanus boninensis)、露兜樹 (Pandanus tector ius)、胡椒科胡椒屬之樹胡椒（Piper ，aduncum)、蔞葉（Piper bet le)、風藤（Piper kadsura)、 ^ 蓽撥（Piper longuro)、卡瓦胡椒（Piper methysticum)、胡椒（Piper nigrum)、假蓽撥（Piper retrofractum)、馬齒莧科馬齒莧屬之大花馬齒寬（Portulaca grandif lora)、馬齒莧（Portulaca oleracea)、傘形科鼠尾草屬之鼠尾草 • (Salvia clevelandii)、苦利普檀薩鼠尾草（Salvia cryptantha)、低木鼠尾草（Salvia fructicosa)、鼠尾草 (Salvia officinalis)、快樂鼠尾草（Salviascl area)、其他成為精油原料之植物等。含二氫茉莉酮（CAS: 1 128-08-01 )之植物可列舉例如柑橘科柑橘屬之白檸檬（Citrus aurantifol ia)、酸橙 (Ci trus aurantium)、佛手柑(Ci trus bergamia)、柚 (Citrus grandis)、八朔柑（Citrus hassaku)、伊予柚 _ (Citrus iyo)、香橙（Citrus junos)、乳橘（Citrus kinokuni)、擰檬（Citrus limon)、圓佛手柑（Citrus medica)、夏橙(Citrus natsudaidai)、葡萄柚（'Citrus paradise)、極柑（Citrus reticulate)、甜橙（Citrus sinensis)、小型柚（大分特產）（Citrus sphaerocarpa)、酸桔（Citrus sudachi)、三寶柑（Citrus sulcata)、橘掛 (Citrus tachibana)、溫州橘（Citrus irnshiu)、夾竹桃科卡利撒屬之卡利撒（Car i ssa carandas)、無刺卡利撒 (Carissa grandif lora)、其他成為精油原料之植物。 21 318986 200800289 含香芹基乙酸酯（CAS : 6380-28-5)之植物可列舉傘形科孜然芹屬之孜然芹（Cum i num cym i num)、馬鞭草科過江藤，屬之橙香馬鞭草（Lippia citriodora)、爪13圭馬鞭草、（Lippia javanica)、過江藤（Lippia nodi f lora)、馬魯佛洛拉馬鞭草（Lippi a malt ufl ora)、唇科百里香屬之迪卡薩圖百里香（Thymus decassatus)、紅花百里香（Thymus sipy leus)、薰衣草百里香（Thymus thracicus)、法國百里 Φ 香（Thymus vulgaris)、西班牙百里香（Thymus zygis)、敗醬科綠草屬之纈草（Valeriana fauriei )、嫩莖線草 (Valeriana flaccidissima)、寬葉織草（Valeriana officinalis)# 〇含有愈瘡奠（CAS: 489-84_9)之植物為：菊科蒿屬之苦蒿（苦艾）（Artemisia absinthium)、青蒿（Artemisia apiacea)、茵陳蒿(Artemisia capi 1 laries)、龍蒿 (Artemisia dracunculus)、白蒿（Artemisia maritime)、 • 五月艾（Artemisia princes)、柏科柏松屬之距花柏松 (Callitris calcarata)、彎垂柏松（Callitris cupressiformis)、 (Cinnamomum burmanni)、棒樹 (Cinnamomum camphor a) - 1¾ (Cinnamomum cassia)、天竺桂（Cinnamomum japonica) i黃樟（Cinnamomum parthenoxylon)、西波帝肉桂（Cinnamomum seiboldii)、錫蘭肉桂（Cinnamomum verum)、番櫻桃科番櫻桃屬之 (Eugenia banderensis)、老鸛科老鸛屬之大根老鸛草 (Geranium macrorrhizura)、尼泊爾老鶴草（Geranium 318986 22 200800289 nepalense)、松節藻科凹頂藻屬之鈍凹頂藻（Laurencia obtusa)、菊科洋甘菊屬之洋甘菊（Matricaria ，chamomilla)、錦葵科箭之根梵天花屬之香法尼葵（Pavonia odorata)等〇含有二氫松柏醇（CAS : 2305-13-7)之植物可列舉松科松屬之赤松（Pinus densiflora)、紅松（Pinus koraiensis)、偃松（Pinus pumila)、歐洲赤松（Pinus 馨 sy lvestris)、松科金葉松屬之金錢松（Pseudolarix amabil i s)等。含有甜菊糖(CAS: 57817-89-7)之植物可列舉菊科甜菊屬之甜葉菊（Stevia rebaudiana)等。含有DL-α -蒎烯（CAS: 2437-95-8)之植物可列舉桃金娘科桉屬之尤加利樹（桉樹）（Eucalyptus amygdal ina)、圓葉尤加利（Eucalyptus cinerea)、藍膠尤加利（Eucalyptus globulus)、白膠尤加利（Eucalyptus leucoxylon)、王桉 _ (Eucalyptus regnans)、其他成為精油原料之植物。馬鞭草苷（CAS: 548-37-8)為另稱為山茱萸苷（Cornin) 之化合物。含有馬鞭草苷之植物可列舉山茱萸科山茱萸屬之雞σ素子（Cornus capitata)、加拿大草茱萸（Cornus canadensis)、燈檯樹（Cornus controversa)、大花四照花 (Cornus f lorida)、四照花（Cornus kousa)、大葉山茱萸 (Cornus macrophy 1 la)、大果山茱萸(Cornus mas)、山茱萸（Cornus officinalis)、玄參科，釣鐘柳屬之釣鐘柳 (Penstemon frutescens A Pentstemon gloxinioides)、 23 318986 200800289 光澤釣鐘柳（Penstemon nitidus)、馬鞭草科馬鞭草屬之美女櫻（Verbena hybrida)、馬鞭草（Verbenaofficinalis)、 .細葉美女櫻（Verbena peruviana)、縫葉美女櫻（Verbena < rigida)、灰白美女樓（Verbena strict a)、宿根美女稷 (Verbena tenera)等。含有1-二十七烧醇（CAS : 2004-3 9_9)之植物可列舉龍舌蘭科龍舌蘭屬之金邊龍舌蘭（Agave americana)、狹葉龍 •舌蘭麻（Agave cantala)、絲龍舌蘭（Agave f ilifera)、灰葉劍麻（Agave fourcroydes)、戟葉龍舌蘭（Agave potatorum)、瓊麻（Agave sisalana)、厚葉龍舌蘭（Agave victoriae-reginae)、葫蘆科西瓜屬之苦西瓜（Citrulius colocynthis)、西瓜（Citrullus lanatus)、豆科盗人萩屬之疏花山螞壇（Desmodium laxif lorum)、羽葉山螞虫皇 (Desmodium oldhamii)、荒地盗人萩（Desmodium paniculatum)、盗人萩（Desmodium padocarpum)、桑科無 ⑩花果屬之象耳槟^Ficus auricu lata)、愛玉子（Ficus awkeotsang) ' 孟加拉榕（Ficus bengalensis)、垂葉榕 (Ficus benjamina)、無花果（Ficus carica)、槲寄生無花果（Ficus diversifolia )、印度橡膠樹（Ficus elastoca)、牛乳榕（Ficus erecta)、琴葉榕（Ficus lyrata)、細葉榕（Ficus microcarpa)、白背肢藤榕（Ficus oxyphylla)、薜篇（Ficus pumila)、菩提樹（Ficus religiosa)、雀榕（FiCUs superba)、埃及無花果（Ficus sycomorus)、菊科郗簽屬之毛梗豨簽（Siegesbeckia 24 318986 200800289 glabrescens)、豨簽（Siegesbeckia oriental is) # o 含有4_羥基香豆素（CAS: 1076-38-6)之微生物可列舉 ,青黴屬之卡門柏青黴（Penici 11 ium camemberti i )、詹氏青 4 黴（Penicillium jensenii)、青黴菌（Penicillium notatum)' 羅克福爾青黴菌（Peniccillium roqueforti) 等。含有4_羥基香豆素之植物可列舉牛栓藤科栗豆藤屬之光澤栗豆藤（Agelaea nitida)、歐利估亞栗豆藤（Agelaea ⑩obligua)、三葉栗豆藤（Agelaea trifolia)、牛栓藤科螯毛屬之可希聶斯（Byrsocarpus coccineus)、牛栓藤科螫毛果屬之五葉螫毛果（Cnestis corniculatus)、尼日利亞螫毛果（Cnestis ferruginea)、傘形科阿魏屬之阿利鉤妮阿魏（Ferula arrigonii)、阿魏草（Ferula assafoetida)、阿魏草（Ferula assafoetida)、大菌香（Ferula communis)、總苞阿魏（Ferula involucrata)、庫西施塔妮卡阿魏（Ferula kuhistanica)、多石阿魏（Ferula 着 lapidosa)、廣羽片阿魏（Ferula latipinna)、白穗阿魏 (Ferula leucographa)、短葉阿魏（Ferula linkii)、席娜依卡阿魏（Ferula sinaica)、孫卡席亞阿魏（Ferula soongarcia)、帖奴依斯塔阿魏（Ferula tenuiesta)、烏崗姆阿魏（Ferula ugamica)、柑橘科芸香屬之小芸香（Ruta chalepensis)、芸香（Ruta graveolens)等。膽酸（CAS : 81-25-4)含於許多哺乳類之膽汁中。含有膽固醇油酸酯（CAS: 303-43-5)之微生物可列舉落磯山革蜱（Dermacentor andersoni)等。 25 318986 200800289M Shortula (Ferula linkii), Ferula sinaica, Ferula soongarcia, Ferula tenuiesta, Ugum Awei ( Ferula ugamica) • Wait. Plants containing ropifelin (CAS: 86992-41-8) can be cited as Ferula arrigoni i, Ferula assafoetida, and Ferula communis. Ferula involucrata, Ferula kuhi stanica, Ferula lapidosa, Ferula 1 at i pinna " White Spike Ferula leucographa), Ferula l inki i, Ferula Φ sinaica, Ferula soongarcia, Ferula tenuiesta, Ferula ugamica and so on. Plants containing geranyl acetate (CAS: 105-87_3) include Abies mariesii, Ageratum houstonianum, and Asteraceae Atractylodes lancea, Baccharis dracuncul i fol 1 ia, Baccharis latifol ia, and willow tree (Baccharis 16 318986) 200800289 salicifollia), Daisy perennis, Calidula of ficinal is, Umbelliferae, Carum carvi, Brassica Caulerpa racemosa, Cestrum nocturnum, Changmium smyrnioides, Citrus auranti fol ia ), Citrus aurantium, Citrus _ bergamia, Citrus grandis, Citrus hassaku, Citrus iyo, Citrus junos, Citrus kinokuni ),lemon (Ci trus 1 imon), Citrus medica, Citrus natsudaidai, Citrus paradise, Citrus reticulate, Citrus sinensis, small pomelo (Oita specialty) Citrus sphaerocarpa), Citrus sudachi, Citrus sulcata, Citrus tachibana, Citrus • unshiu, Codium tomentosum, Umbelliferae Cuminum cyminum, Cymbopogon caesius, Cymbopogon ci trains, Cymbopogon khasi anus, Cymbopogon winterianus ), the genus Daucus Carota, the genus Dracocephalum kotschyi, the Eucalyptus urophylla, the genus Helichrysum Helichrysuin bracteatum, Houttuynia 17 3189S6 200800289 cordata, octagonal octagonal octagonal Η 1 i cium griffithii, 滇西八角(Illicium merrillianum), Cypress cypress, Jimiterus f〇rmosana, Juniperrus, rigida, and Javanese genus of Myrtaceae (1^?1:〇3?6 〇1_ javanicum), Ligusticum mutellina, Lipiria javanica, genus Verbena, summer sorghum Origanum rotundif〇1 ium, geranium geranium (Pelargonium graveolens), arboreal genus (Ph〇ebe porosa), rhododendron hibiscus Rhododendron primulaef lorum, Rosa centifolia, Rosaceae, Salvia clevelandi i, Salvia fructicosa ), Salvia officinalis, Salvia sclarea, Compositae Φ marigold genus Tagetes erecta, Terminian bentzoe, North Korean cypress Thuja koraiensis, lip Thymus sipyleus, Thymus thracicus, Thymus vulgaris, Thymus zygis, Zanthoxylum bungeanum, Zanthoxylum schinifolium, etc. . Plants containing nerolidol (CAS: 7212-44-4) can be found in Achi 1 lea alpine, Achi 1 lea 18 318986 200800289 filipendulina, Liguria AchiHea ligustica, Achillea mi lie i folium, Achillea ▲ moschata, Achi 1 lea ptarmica, Achi 1 lea ¥ tomentosa, Asteraceae Ageratuin conyzoides, Ageratum houstonianum, Amomum aromaticum, Am(10)um cardam(10)um, Amomum subulatum, sand Amomum xanthioides, Amomum zingiber, Amorpha fruticosa, Camelli ia sinensis, Camellia genus, Canavalia Ensiformis), Canavalia gladiata, Canaval ia maritima, Cestrum aurant iacum, Cestrum nocturnum, Cestrum purpureum ) Citrus auranti fol ia, Citrus aurantium, Citrus bergamia, Citrus grandis, Citrus hassaku, Citrus iyo ), Citrus junos, (Citrus kinokuni) ' ##(Citrus limon), Citrus medica, Citrus natsudaidai, Citrus paradise, Citrus reticulate, Citrus sinensis, Citrus sphaerocarpa, Citrus sudachi, Citrus sulcata, Citrus tachibana, Citrus unshiu, Eucalyptus The genus is Croton 19 318986 200800289 aubrevi 1 lei, Croton el uteri a, Croton japonicum, Croton sebiferus, Croton tigl ium , Croton zambesicus, Daucus carota, Durio zibethinus, and the genus of the genus Elish Oltzia ci 1 iata), Elsholtzia rugulosa, Euphoria longan, Ginkgo biloba, Ginkgo biloba, Juniperus communis , Juniperus conferta, Juniperus formosana, Juniperus rigida, Ligusticum mutellina, verbena branch of the genus Verbena (Lippia alba) ), Lippia ci triodora, Lippia javanica, Lippie nodi f 1 ora, Lippia mal tuf 1 ora, Umbelliferae Mentha aquatica, Mentha arvensis, Mentha canadensis, Mentha longi folia, Mentha piperita, Menthapulegium, Mentha spicata Micromeria carminea, Mi mu sops e 1 engi, and basil of the genus Umbelliferae of the genus Umbellifera (the genus Umbellifera) Ocimum basil icum), Brazilian vanilla basil (〇cimum kilimandoscharicum), fine flower basil (〇ciniuni tenuiflorum), umbrella open, Oenanthe javanica, Pandanus branch, 20 338986 200800289 Pandanus boninensis, Pandanus tector ius, Piper, aduncum, Piper bet le, Piper kadsura, ^ ( ( Piper longuro), Piper methysticum, Piper nigrum, Piper retrofractum, Portulaca grandif lora, Purslane Portulaca oleracea), Salvia clevelandii, Salvia cryptantha, Salvia fructicosa, Salvia officinalis , Salviascl area, other plants that become essential oils. Plants containing dihydrojasmone (CAS: 1 128-08-01) include, for example, Citrus aurantifol ia, Citrus aurantium, Citrus bergamia, and pomelo. (Citrus grandis), Citrus hassaku, Citrus iyo, Citrus junos, Citrus kinokuni, Citrus limon, Citrus medica, summer Orange (Citrus natsudaidai), grapefruit (Citrus paradise), Citrus reticulate, Citrus sinensis, Citrus sphaerocarpa, Citrus sudachi, Citrus (Citrus) Sulcata), Citrus tachibana, Citrus irnshiu, Car i ssa carandas, Carissa grandif lora, and other essential oils plant. 21 318986 200800289 Plants containing carvyl acetate (CAS: 6380-28-5) can be cited as Cum i num cym i num, Verbenaceae, and genus Lippia citriodora, larvae 13 (Lippia javanica), Lippia nodi f lora, Lippi a malt ufl ora, lip thyme Thymus decassatus, Thymus sipy leus, Thymus thracicus, Thymus vulgaris, Thymus zygis, and genus Valeriana fauriei, Valeriana flaccidissima, Valeriana officinalis # 〇愈 CAS (CAS: 489-84_9) The plant is: Artemisia argyi (Artemisia scoparia) (Artemisia absinthium), Artemisia apiacea, Artemisia capi 1 laries, Artemisia dracunculus, Artemisia maritime, • Artemisia princes, Cypress Flower Callitris calcarata, Callitris cupressiformis, (Cinnamomum burmanni), Cinnamomum camphor a - 13⁄4 (Cinnamomum cassia), Cinnamomum japonica i Cinnamomum parthenoxylon, West wave Cinnamomum seiboldii, Cinnamomum verum, Eugenia banderensis, Geranium macrorrhizura, Geranium 318986 22 200800289 nepalense), Laurencia obtusa of the genus Eucalyptus, Matricaria (chamomilla) of the genus Compositae, and the root of the genus Brassica Pavonia odorata) is a plant containing dihydroconiferol (CAS: 2305-13-7), which can be cited as Pinus densiflora, Pinus koraiensis, Pinus pumila, and European red pine (Pinus densiflora). Pinus sylvester, Pseudolarix amabil is, etc. The plant containing stevioside (CAS: 57817-89-7) may, for example, be Stevia rebaudiana of the genus Stevia rebaudiana. Plants containing DL-α-decene (CAS: 2437-95-8) include Eucalyptus amygdal ina, Eucalyptus cinerea, and blue eucalyptus Eucalyptus globulus, Eucalyptus leucoxylon, Eucalyptus regnans, and other plants that are essential oils. Verbenaside (CAS: 548-37-8) is another compound known as Cornin. Plants containing verbena glycosides include Cornus capitata, Cornus canadensis, Cornus controversa, Cornus f lorida, and four flowers (Cornus kousa), Cornus macrophy 1 la, Cornus mas, Cornus officinalis, Scrophulariaceae, Penstemon frutescens A Pentstemon gloxinioides, 23 318986 200800289 Penstemon nitidus, Verbena hybrida, Verbenaofficinalis, Verbena peruviana, Verbena < rigida, gray-white beauty Verbena strict a, Verbena tenera, etc. Plants containing 1-27 decyl alcohol (CAS: 2004-3 9_9) include Agave americana, Agave cantala, Agave americana, Agave cantala, Agave f ilifera, Agave fourcroydes, Agave potatorum, Agave sisalana, Agave victoriae-reginae, Cucurbitaceae Citrusus colocynthis, Citrusolus lanatus, Desmodium laxif lorum, Desmodium oldhamii, Desmodium paniculatum, Desmodium padocarpum, Sanko no 10 flower genus Ficus auricu lata, Ficus awkeotsang 'Ficus bengalensis, Ficus benjamina, fig (Ficus carica) , Miscus diversifolia, Ficus elastoca, Ficus erecta, Ficus lyrata, Ficus microcarpa, Ficus oxy Phylla), Ficus pumila, Ficus religiosa, FiCUs superba, Ficus sycomorus, Phyllostachys pubescens (Siegesbeckia 24 318986 200800289 glabrescens), 豨(Siegesbeckia oriental is) # o Microorganisms containing 4_hydroxycoumarin (CAS: 1076-38-6) can be enumerated, Penicillium carbendazim (Penici 11 ium camemberti i ), Penicillium jensenii ), Penicillium notatum 'Peniccillium roqueforti' and the like. The plant containing 4-hydroxy coumarin may be exemplified by Agelaea nitida, Agelaea 10obligua, and Agelaea trifolia, of the genus Chrysanthemum. Byrsocarpus coccineus, Cnestis corniculatus, Cnestis ferruginea, Umbelliferae Ferula arrigonii, Ferula assafoetida, Ferula assafoetida, Ferula communis, Ferula involucrata, Cousy Stanika Ferula kuhistanica, Ferula (lapidosa), Ferula latipinna, Ferula leucographa, Ferula linkii, Siena Ekawei (Ferula sinaica), Ferula soongarcia, Ferula tenuiesta, Ferula ugamica, Ruta chalepensis, citrus Musk (Ruta grave Olens) and so on. Cholic acid (CAS: 81-25-4) is contained in the bile of many mammals. Examples of the microorganism containing cholesterol oleate (CAS: 303-43-5) include Dermacentor andersoni. 25 318986 200800289

含有柊皮苷（CAS: 524-30-1)之植物可列舉槭樹科槭屬之潔槭（Acer aidzuense)、大紅葉槭（Acer amoenum)、銳齒槭（Acer argutum)、三角槭（Acer buergerianum)、藏南槭（Acer campestre)、青皮槭（Acer carpinif〇1 ium)、梓葉槭（Acer crataegi f ol ium)、鬼槭（Acer diabolicum)、二柱槭（Acer distylum)、茶條槭（Acer ginnala)、羽扇槭 (Acer japonica)、五角楓（Acer mono)、複葉槭（Acer negundo)、黑槭（Acer nigrum )、曰光槭（Acer nikoense)、曰本槭（Acer nipponicum)、青楓(Acer palmatim)、挪威槭（Acer platanoides)、歐亞槭（Acer pseudoplatanus)、密花槭（Acer pycnanthum)、褐脈槭（Acer rufinerve)、銀槭（Acer saccharum)、塞波得槭（Acer sieboldianum)、毛脈槭（Acer tschonoskii)、花楷槭（Acer ukurunduense)、七葉樹科七葉樹之七葉樹（Aesculus chinensis)、歐洲七葉樹（Aesculus hippocastanum)、曰本七葉樹（Aesculus turbinata)、本犀科白瑕屬之美國白臘（Fraxinus americana)、大葉白虫鼠（Fraxinus chinensis)、歐洲白臘 (Fraxinus excelsior)-光虫鼠樹（Fraxinus gri f f i thi i)、茚可菲拉（Frax inns hynchophy 1 la)-曰本白臘（Fraxinus japonica)、絨毛白蝶（Fraxinus lamiginosa)、滿州白虫鼠 (Fraxinus mandshurica)、歐納白蠛（Fraxinus ornus)、銳葉白壌（Fraxinus oxyphyl la)"青木（Fraxinus spaethi ana)、施寶白躐（Fraxirms sieboldiana)、杜鸦花科藍莓屬之矮叢藍莓（Vaccinium angust i fol ium)、兔眼藍 26 318986 200800289 莓（Vaccinium ashei)、澳洲越橘（Vaccinium australe)、Plants containing quercetin (CAS: 524-30-1) include Acer aidzuense, Acer amoenum, Acer argutum, and Acer. Buergerianum), Acer campestre, Acer carpinif〇1 ium, Acer crataegi f ol ium, Acer diabolicum, Acer distylum, Acer maple (Acer ginnala), Acer japonica, Acer mono, Acer negundo, Acer nigrum, Acer nikoense, Acer nipponicum, Qingfeng (Acer palmatim), Acer platanoides, Acer pseudoplatanus, Acer pycnanthum, Acer rufinerve, Acer saccharum, Acer sieboldianum ), Acer tschonoskii, Acer ukurunduense, Aesculus chinensis, Aesculus hippocastanum, Aesculus turbinata, Ben Rhododendron Fraxinus americana, Fraxinus chinensis, Fraxinus excelsior-Fraxinus gri ffi thi i, Frax inns hynchophy 1 la-曰Fraxinus japonica, Fraxinus lamiginosa, Fraxinus mandshurica, Fraxinus ornus, Fraxinus oxyphyl la & 青青 (Fraxinus spaethi) Ana), Fraxirms sieboldiana, Vaccinium angust i fol ium, rabbit eye blue 26 318986 200800289 Vaccinium ashei, Vaccinium australe,

南燭（Vaccinium bracteatum)、毛果藍苺（Vaccinium hirtum)、毛蕊花（Vaccinium japonicum)、蔓越莓 (Vaccinium macrocarpon)、歐洲越橘（Vaccinium myrtillus)、腺齒越橘（Vaccinium oldhami)、小紅莓 (Vaccinium oxycoccus)、佳美越橘（Vaccinium praestans)、史莫月橘（Vaccinium smallii)、篤斯越橘 (Vaccinium uliginosum) 、lx 豆越才高(Vaccinium vitis-idaea)、忍冬科紅王子錦帶屬之紫葉錦帶（Weigela florida)等。含有佛手酚（CAS: 486-60-2)之植物可列舉柑橘科柑橘屬之白檸檬（Citrus aurantifolia)、酸橙（Citrus aurantium)、佛手柑（Citrus bergamia) ' 柚（Citrus grandis)、八朔柑（Citrus hassaku)、伊予柚（Citrus iyo)、香橙（Citrus junos)、乳橘（Citrus kinokuni)、檸檬（Citrus limon)、圓佛手甜（Citrus medica)、夏橙 (Citrus natsudaidai)"葡萄柚（Citrus paradise)、握柑 (Citrus ret iculate)、甜橙（Citrus sinensis)、小型柚 (Citrus sphaerocarpa)、酸桔（Citrus sudachi )、三寳柑 (Citrus sulcata)、橘柑（Citrus tachibana)、溫州橘 (Citrus unshiu)、桑科臭桑屬之厚葉盤花木（Dorstenia contra jerva)、橢圓增桑（Dorstenia el 1 iptica)、桑科無花果屬之象耳榕（Ficus auriculata)、愛玉子（Ficus awkeotsang)、孟力口拉樓（Ficus bengalensis)、垂葉標： 27 338986 200800289 (Ficus ben jamina)、無花果（Ficus car ica)、懈寄生無花果（Ficus diversif〇iia )、印度橡膠樹（Ficus elastoca)、牛乳稼（Ficus erecta)、琴葉榕（Ficus lyrat a)、細葉榕（Ficus microcar pa)、白背攸藤榕（Ficus oxyphylla)、薜荔（Ficus pumila)、菩提樹（Ficus religiosa)、雀榕（Ficus SUperba)、埃及無花果（FicusVaccinium bracteatum, Vaccinium hirtum, Vaccinium japonicum, Vaccinium macrocarpon, Vaccinium myrtillus, Vaccinium oldhami, Cranberry (Vaccinium oxycoccus), Vaccinium praestans, Vaccinium smallii, Vaccinium uliginosum, lx Beans (Vaccinium vitis-idaea), Lonicera edulis The Weigela florida and the like. Plants containing bergamot (CAS: 486-60-2) include Citrus aurantifolia, Citrus aurantium, Citrus bergamia 'Citrus grandis, and sassafras. (Citrus hassaku), Citrus iyo, Citrus junos, Citrus kinokuni, Citrus limon, Citrus medica, Citrus natsudaidai & grapefruit (Citrus paradise), Citrus ret iculate, Citrus sinensis, Citrus sphaerocarpa, Citrus sudachi, Citrus sulcata, Citrus tachibana, Wenzhou orange (Citrus unshiu), Dorstenia contra jerva, Dorstenia el 1 iptica, Ficus auriculata, Ficus awkeotsang , Ficus bengalensis, lobes: 27 338986 200800289 (Ficus ben jamina), fig (Ficus car ica), figurine fig (Ficus diversif〇iia), Indian rubber tree (Ficus elastoca), Ficus erecta, Ficus lyrat a, Ficus microcar pa, Ficus oxyphylla, Ficus pumila, Ficus religiosa , Ficus SUperba, Egyptian figs (Ficus

sycomorus)、傘形科晃活屬之晃活（Notopterygium franchetii)、狹葉晃活（Notopterygium incisum)、寬葉晃活（Notopterygium rhizomes)等。奉發明之維生素C 萄柚、龍鬚草、西洋常春藤、石鹼草 «3 桃仁、'繡球花——μ蜗干 '四于.吊|膝、石鹼魚腥草、洋甘菊、兒茶、金縷梅、桑、米糠、問莉等等可單獨使用1種，亦可將2種以上組合使甩。又，該= 植物可使甩其粉碎物、其萃取物或萃取水解物之任何_^種寺厚朴（Magnolia obovata)為木蘭科木蘭屬之落葉言。木“刀布於曰本北 >母返至九州及中國，有出生於山地植到庭園樹、公園樹建築劑等裁培。學名亦稱為广 hyP〇leUCa。藥用部位之樹皮含有生物驗之木蘭箭毒驗1& 〇nagn〇CUrarine)、木蘭花驗（叫_。心）、木聚糖夕木蘭醇、厚朴⑽⑽呢⑷^可用於缓和 ^之噁心等症狀。 ^ m ^ 扁桃仁（Prunus dulcis)发 # * 。 w為嗇薇科櫻屬之落葉高太，合生育於夏季雨少之地域之溫暖水土。現在為以：木適岸各國及美國加州為主產地之植物。學名亦口 318986 200800289 amygdalus 、 Prunus communis 、 Amygdalus dulcis 、Sycomorus), Notoptery gium franchetii, Notoptery gium incisum, Notoptery gium rhizomes, etc. Invented vitamin C grapefruit, Eulaliopsis, Western ivy, Shigaki grass «3 peach kernel, 'hydrangea flower - μ worm dry' four in. hanging | knee, stone alfalfa, chamomile, catechu, The witch hazel, the mulberry, the rice bran, the lily, and the like may be used alone or in combination of two or more. In addition, the plant can make any of its pulverized material, its extract or extract hydrolyzate, Magnolia obovata, a leaf of Magnoliaceae. The wood "knife cloth in Sakamoto North" is returned to Kyushu and China. It is born in the mountains and planted in the garden tree, park tree building agent, etc. The scientific name is also known as Guang hyP〇leUCa. The bark of the medicinal part contains organisms. Test Magnolia arrow poison test 1 & 〇nagn 〇 CUrarine), magnolia test (called _. heart), xylan sulphate, magnolia (10) (10) (4) ^ can be used to ease the symptoms of nausea and other symptoms. ^ m ^ almond Prunus dulcis hair # * . w is the deciduous tree of the genus Rosaceae, which grows in the warm water and soil of the region with less rain in summer. It is now the plant of the wood-based countries and California. The scientific name is also 318986 200800289 amygdalus, Prunus communis, Amygdalus dulcis,

Amygdalus communis、Amygdalus sativus。將種子壓搾獲 ‘得=油可利用於製造乳化劑' 按摩油、香料、甜香酒、以 •及製菓等。甜扁桃仁由於未含有氰酸配糖體之杏素 (amygdalin)而可生食，可利用奶油、鹽等調味作成堅果。 —，球花（Hydrangea macr〇phyl丨a)為虎耳草科繡球屬 f落葉低木，可作為庭木或藥用栽培之植物。植物體呈甜 ❿味，可代替砂糖使用。明瞭甜味成分為葉甜素 (Phyllodulcin)、異葉甜素，乾燥葉可作為甘茶飲用。葡萄柚（Citrus paradise)為柑橘科柑橘屬之植物，為在暖地栽培之常綠高木。果肉柔軟多汁，含有檸檬酸^至 2%，酸味稍強，有源自檸檬苦素（lim〇nin)之苦味，大小為約夏橙大小。肉色依品種而異，為黃色至紅色。紅色係因番茄紅素。在利用上可生食或作成果汁，大都加工作成冷凍濃縮果汁。，遽鬚草（Ophiopogon jponicus)為百合科沿階草屬之蕨類植物，為分布於北海道至九州及中國、韓國之植物。別名為沿階草，根部膨大部分稱為麥門冬，麥門冬利用於滋養強狀、鎮咳、袪痰等。、常春藤（Hedera helix)為五加科常春藤屬，為廣泛分布於北非之植物。別名洋常春藤（English Ivy)。葉可用於治療結石症、黃疸等。 ' 石鹼草（Saponiria off icinalis)為石竹科肥皂草屬之植物，為原產於歐洲之多年草。別名為肥皂草 318986 29 200800289Amygdalus communis, Amygdalus sativus. The seeds are pressed to obtain 'oil = oil can be used in the manufacture of emulsifiers' massage oil, spices, sweet liqueur, and fruit. Sweet almonds can be eaten raw by the amygdalin which does not contain cyanate glycosides, and can be made into nuts by seasoning with cream and salt. —, Hydrangea macr〇phyl丨a is a deciduous genus of the genus Saxifragaceae. It is a plant that is cultivated in the wood or medicinal. The plant has a sweet astringent taste and can be used instead of sugar. It is clear that the sweet ingredients are Phyllodulcin and isoflavone, and the dried leaves can be used as sweet tea. Citrus paradise is a citrus citrus plant, an evergreen tall wood cultivated in warm fields. The flesh is soft and juicy, containing citric acid to 2%, slightly sour, and has a bitter taste derived from limonin, which is about the size of summer orange. The color of the meat varies from species to yellow to red. Red is lycopene. In the use of raw food or juice, most of them work into frozen concentrated juice. Ophiopogon jponicus is a fern of the genus Liliaceae, and is a plant distributed in Hokkaido to Kyushu and China and Korea. The alias is the grass along the order, the root swelling is mostly called Maimendong, and Maimendong is used to nourish strong, antitussive, expectorant and so on. Hedera helix is a genus of the genus Ignition, which is widely distributed in North Africa. Alias Ivy. Leaves can be used to treat calculus, jaundice, etc. 'Saponiria off icinalis is a plant of the genus Salvia, and is a multi-year grass native to Europe. Alias is saponin 318986 29 200800289

Soapwort)。又，亦以學名 Si lence sap〇nal ia 表示。作為藥用，葉或根莖可用於濕療等皮膚病。於歐洲，自古即作 …為肥皂之替代品使用。 ★ 魚腥草（Houttuynia cordata)為三白草科蕺菜屬之植物，為分布於本州 '四國、九州、沖繩及台灣、中國、喜瑪拉亞山、爪哇等之多年草。蕺菜之地上部乾燥物可作^ 解熱、解毒、利尿、濕疹、消炎藥使用。已知其特有之臭氣係來自月桂駿或癸酸等搭類。在亞洲，利用作為藥用了在歐美則作為觀賞用栽培。 …洋甘韻（Matricaria chamomilla)為原產於歐洲之菊科年甘菊屬之1年草植物。別名亦稱為白花母菊、甘菊、日爾曼甘菊、德國甘菊、洋甘菊。以藥用為目的在以歐洲各地為首之溫暖地帶栽培，藥用部位之頭花係以發汗、驅蟲為目的而使用，從洋甘菊取得之精油可利用於賦予甜香酒或香煙香氣、香水、洗髮精。兒余鉤藤（Uncalia gambir)為茜草科鉤藤屬之植物，分布於馬來半島、斯瑪德拉、婆羅料，在東南亞各地栽培之藤性常綠低木。藥料位係使用葉或小枝，用於緩和血便、血尿、血_，又，亦作為口内清涼劑之原料使用。在同=之目的亦可使用兒茶（Acaci a Catechu)之木心。 ^金縷梅（Hamameiis virginina)為原產於北美東部至陶。F之金縷科金续屬之植物，葉子在印度有利用作為藥用茶=歷史，可用於下渴劑、痒瘡。已知葉或樹皮含有金縷 318986 30 200800289 桑（Morus alba)為原產於東南亞之桑科桑屬植物，別名冬桑、白桑、桑白（whitemulberry)。在漢方，幼枝稱為 .柔枝’葉稱為柔葉，果實稱為柔椹子，根之皮稱為柔白皮。 v柔枝可用於風濕性關節炎或神經痛等，柔葉可用於解熱，木椹子可用於滋養強狀、貧血、養毛，柔白皮可用於消炎性利尿、鎮咳、高血壓。之糠米糠為稻科稻屬（Oryza sativa)之植物日本主食之米問荊（equi setum arvense)為木賊科木賊屬之蕨類植 $ :其營養莖稱為問荊，胞子莖稱為土麻黃。問荊及土麻出自地下災’土麻黃於初春成長，之後問荊展開。問刑作為樂用可於利尿、止血、鎮咳、解熱服用，亦可作為外料皮膚m料。 7作明列舉之本發明所發現之化合物之植物及本發明之植物，其葉、贫、# ^ 地上部分及根下二木質部、木皮部（樹皮）等均可使用。又，含;t 種子、樹脂等所有部位生物可使用例如㈣;;=舉之树明發狀化合物之微絲體、子實體等物'固體培養物，為菌類時，菌物、微生物、所發現之化合物之植物、礦取物之水解物使用1肢三原體之水解物、萃取物、萃化合物。，該等化合物亦可使用化學合成之從植物、礦物微生物動物獲得本發明化合物之萃 318986 3] 200800289 :方仏可列舉例如為葡萄柚時，將葡萄柚乾燥之乾敗粉㈣萄柚壓搾獲得之搾汁等以適當之溶：萃，取視^況貫施水解處理，以過濾等除去 w 泡液除去溶劑後以管柱 :夕、知/ σ *，從該的化合物之方法。層析法寺逍常之精製手段’獲得目萃取使用之溶劑可列舉甲醇、乙醇、丙醇、Μ 醇等醇類，、丙，、乙酸乙酯、己烷、環己产，：-烷、氯仿等有機溶劑，水等疋、一虱甲 2種以卜、曰人从該寻洛劑可早獨使用，亦可為5至9^ /。萃取條件並無特別限制，例如溫度宜 ;為常严、厂乂好為15至阶’在常溫亦宜萃取。《力了為吊£、加壓、減壓中之任一者。蓋取〜力劑而異，為數分鐘至數小時，妹反舜广廷疋之溶又，進行水解時，可列之分解、二：：由乳酸菌或酵母菌等微生物刀解i由鹽酸、硫酸、乙酸、_ 勿酒石酸、富馬酸、蘋果酸等之酸分解、氣化鉀等之驗分解等。水解1μ化鈉、氫素或料生物— a 木件亚無特別限制，使用酵 ;=以配合所使用之酵素或微生物之最適'、广又、pH值，通常係於1〇至55。〇酿至24小時。使用酸或驗進行時，、=、進行水解約5 ^ 迷常以2至15#旦之浪度’於常溫至6代、進行水解約〇 5至24 , f里該等萃取物或萃取物之水解物，。後以珠結乾燥、減壓蒸館、減壓.直=進行PH調整公知之方法，如、曲π, /、二乾燦、，霧乾燥等即可獲㈣縮乾燥物。精製處理方法並無特 318986 32 200800289 可將經由順相及逆相層析再丹》儿殿、脫多虛：E田 n,s ^ . ττ、口日日 _ 土、脫六、處理等組合、精製。之植2=上述方法萃取之葡萄柚萃取物之本發明列舉 ^植物卒取物及本發明列舉之化合物，使用細胞著促進維生素c轉運體之表現。根據此，本發明成物“轉運體生隸㈣何作騎生素€吸收用組成物、吴白用組成物、膠原產生用、组成物使用。Soapwort). Also, it is also represented by the scientific name Si lence sap〇nal ia. As a medicinal, leaves or rhizomes can be used for skin diseases such as wet treatment. In Europe, it has been used since ancient times... as a substitute for soap. ★ Houttuynia cordata is a plant of the genus Amaranthus, which is distributed in the four countries of the state of Shikoku, Kyushu, Okinawa and Taiwan, China, Himalayas, and Java. The dry matter on the top of the leek can be used for heat, detoxification, diuresis, eczema, and anti-inflammatory drugs. It is known that its characteristic odor is from yujun or tannic acid. In Asia, it is used as a medicinal product. ...Matricaria chamomilla is a 1-year grass plant of the genus Chamomile, which is native to Europe. The alias is also known as white flower chrysanthemum, chamomile, Germanic chamomile, German chamomile, and chamomile. For the purpose of medicinal use, it is cultivated in a warm zone headed around Europe. The head of the medicinal part is used for sweating and deworming. The essential oil obtained from chamomile can be used to impart sweet aroma or cigarette aroma, perfume, Shampoo. Uncalia gambir is a plant of the genus Uncaria, which is distributed in the Malay Peninsula, Smadea, and Borneo. It is cultivated in the Southeast Asia. The leaves are used to relax bloody stools, hematuria, and blood, and are also used as raw materials for oral fresheners. The heart of Acaci a Catechu can also be used for the same purpose. ^ Hamameii virginina is native to eastern North America to Tao. The plant of F. genus is a plant of the genus, and the leaves are used in India as a medicinal tea = history, which can be used for lower cravings and itch. It is known that leaves or bark contain gold 缕 318986 30 200800289 Morus alba is a genus of Mulberry, which is native to Southeast Asia, and is also known as winter mulberry, white mulberry, and white mulberry. In the Chinese side, the young branches are called . The soft leaves are called soft leaves, the fruits are called soft scorpions, and the roots are called soft white skins. v soft branches can be used for rheumatoid arthritis or neuralgia, soft leaves can be used for antipyretic, wooden scorpions can be used to nourish strong, anemia, hair growth, soft white skin can be used for anti-inflammatory diuretic, antitussive, high blood pressure. The rice glutinous rice is the plant of the genus Oryza sativa. The Japanese staple food equisetum arvense is a fern of the genus Equisetum. The vegetative stem is called the stalk, and the stem is called the stalk. yellow. Questioning Jing and 麻麻 From the underground disaster, Tu Mahuang grew up in the early spring, and then asked Jing to start. As a pleasure, it can be used for diuresis, hemostasis, antitussive, antipyretic, or as an external skin material. The plants of the compounds of the present invention and the plants of the present invention are exemplified by the leaves, the lean, the # ^ aerial part and the sub-root xylem, the veneer (bark), and the like. In addition, all parts of the t seed, resin, and the like can be used, for example, (4);; = the microfilaments, fruit bodies, etc. of the hairy compound of the tree, solid culture, when the fungus is a fungus, a microorganism, a The hydrolysate of the plant or mineral extract of the compound found is a hydrolyzate, an extract, and an extract compound of the trisomy. These compounds can also be obtained by chemically synthesizing the compound of the present invention from plant or mineral microbial animals. 318986 3: 200800289: For example, when grapefruit is used, the grapefruit dried dry powder (4) grapefruit is obtained by pressing. The juice or the like is appropriately dissolved and extracted, and the hydrolyzed treatment is carried out by filtration, and the solvent is removed by filtration or the like, and the solvent is removed by a column: 、, 知 / σ *, from the compound. The solvent used for the extraction of the extracting method of the temple is exemplified by alcohols such as methanol, ethanol, propanol and decyl alcohol, and C, ethyl acetate, hexane, and cyclohexyl, :-alkane, chloroform. Such as organic solvents, water and other cockroaches, one armor, two kinds of 以, 曰 people from the locating agent can be used alone, can also be 5 to 9 ^ /. The extraction conditions are not particularly limited, for example, the temperature is suitable; it is usually strict, and the factory is preferably 15 to order. "For any of the lifting, pressing, and decompression." Covering ~ force agent varies, for a few minutes to a few hours, the sister 舜舜疋疋疋舜 , , , , , , , 舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜舜Acid decomposition, acetic acid, _ tartaric acid, fumaric acid, malic acid, etc., acid decomposition, etc. Hydrolysis of 1μ sodium, hydrogen or biomass - a wood sub-area is not particularly limited, using yeast; = to match the enzymes or microorganisms used in the optimum ', wide, pH, usually between 1 〇 to 55. 〇 Brewed for 24 hours. When using acid or test, =, hydrolysis is about 5 ^, often with a wave of 2 to 15 #旦, at room temperature to 6 generations, hydrolysis is about 5 to 24, f such extracts or extracts Hydrolyzate, After that, the beads are dried, steamed under reduced pressure, and decompressed. Straight = pH adjustment. Known methods, such as π, /, two dry, fog drying, etc., can be obtained (4) shrinking dry matter. There is no special treatment method for 318986 32 200800289. It can be combined with cis phase and reverse phase chromatography to re-dan, children's temple, detached imaginary: E Tian n, s ^ . ττ, mouth day _ soil, off six, treatment and other combinations ,refined. The invention of the grapefruit extract extracted by the above method is exemplified by the plant pawn and the compound of the present invention, and the use of cells promotes the performance of the vitamin C transporter. According to the present invention, the "transporter of the present invention" (four) is used as an ingredient for the absorption of the stimulating substance, a composition for numbness, a composition for collagen production, and a composition.

發明之「維生素C轉運體生產促進劑」係指提高納 "維生素C轉運體1型（SVCTlht 2 S(SVCT2)之任一者或-者之表現者，換言之，係提高SLC23A1或所示基因中任一者或二者之產物之表現。本發明之「維生素C吸收促進用組成物」係指將維生素C轉運體生產促進劑與至少—種以上之維生素c或維生素C衍生物加以組合者，係提高經口服或經由塗抹攝取之維生素C或其衍生物在標的臟器内之維生素c濃度之組成物0 本發明之「維生素C衍生物」除了 2—〇-a—D-葡萄吡喃糖基-抗壞丙酸外，可列舉L—抗壞血酸單硬脂酸酯、L-抗壞血酸單棕櫚酸酯、L-抗壞血酸單油酸酯等抗壞血酸單烷基酯類，L-抗壞血酸單磷酸酯、L-抗壞血酸—2-硫酸等抗壞血酸單酯衍生物、L-抗壞血酸二硬脂酸酯、L-抗壞血酸一棕櫚酸酯、L-抗壞血酸二油酸酯等抗壞血酸二烧基酯通’ L-抗壞血酸一填酸1旨等抗壞血酸二g旨衍生物、l-抗壞皿酸二硬脂酸醋、L-抗壞血酸三棕櫚酸醋、L-抗壞血酸三 33 3189S6 200800289 油酸酉旨等抗壞血酸三燒壞血酸三酯衍生物等， ' L—抗壞血酸三磷酸酯等抗一酸與各種鹼之鹽。可㈣’ ^限於此。鹽可列舉抗壞血 •金屬鹽(例㈣鹽、鎮屬鹽(例如納鹽)、鹼土本發明…二惟’不只限於此。具淡化作用之心Γΐ成物」係指對既有之肝斑·雀斑過剩、沉…果二：斑·雀斑為黑色素㈤-in)生產曰、。果已知維生素C係盥里色本决忐玄+7? 士關之酪胺酸酶之抑制物 p t、…色$生成雄、切相使成為還原型淺色:；之=有，進氧化型深色黑色素肝斑•雀斑等色本Γ、ί：之作用。在臨床上有改善既有之寻邑；3W儿歲之報告（非皇㈣ 2Η7)，725-729) 用獻7:臨皮 1967, 皮膚細胞中維生^ 物係經由提高組成物。本發明之美白用^ ^ ^生素C之美白作用之素C吸故佤、# 、、且成物係只要含有本發明之維生 c、吸收促進：用用組:物者即可，又，只要不會抑制維生素收促、作用，亦可添加其他添加物加以調整。本發明之「勝；& 有之膠原產生促進作係指經由維生“具 J /主王1疋進作用，藉由提喜曲者。例如膠原產生細胞為皮膚時，：:二= 之組忐物，搬広士 · 1与敌回肌膚濕潤度之组成物 >太1細胞為骨形成細胞時’則為增強骨強 =生1Γ 之㈣產生用組成物只要含有本發明促進用組成物即可，又，只要不會抑卿〜、作用，亦可添加其他添加物加以調整。者「葡糖苷酶劑」係指本身具有葡糖苷酶活性者’或為使葡糖芽酶活性活性化者。本身具有葡糖⑽活 318986 34 200800289 性者可列舉例如酵母、雙叉桿菌、乳酸菌，亦可使用源自鏈球菌屬、麴菌屬、鏈黴菌屬、根霉屬、梭菌屬、鏈球菌 -屬等微生物之葡糖苷酶，惟，不限定於此。活化葡糖苷酶活性之物可列舉例如菊科紫錐花屬之紫錐花（Echinacea 猶 purpurea、Echinacea angustifolia、Echinacea pallida、 Echinacea simuiata - Echinacea paradoxa 、 Echinacea atrorubens)等（專利文獻1 :特開2005-029546號公報）， ⑩惟，不限定於此。本發明之葡糖苷酶劑可將上述材料以原狀粉碎使用，亦可使用上述材料之萃取物、萃取水解物。本發明之劑或組成物可作為食品、醫藥品、化粧品等使用。作為食品，除了通常之食品外亦可作為營養補助食品、機能性食品、健康食品、特定保健用食品等使用，例如可配合於果汁類飲料中。有關為醫藥品時之投予方法，於將有效成分經口服投予，非經口投予時，可與適合直腸内投予、注射等投予方 • 法之固體或液體之醫藥用無毒性載體混合，以慣用之醫藥製劑形態投予。製劑形態可列舉例如粉末、散劑、顆粒、錠劑、膠囊等固形劑、溶液劑、懸濁劑、乳劑等液劑，凍結乾燥製劑等，該等製劑可經由通常之方法調製。上述醫藥品用無毒性載體可列舉例如葡萄糖、乳糖、蔗糖、澱粉、甘露糖醇、糊精、脂肪酸甘油酯、聚乙二醇、羥基乙烯澱粉、乙二醇、聚氧伸乙基山梨糖醇酐脂肪酸酯、胺基酸、明膠、白蛋白、水、生理食鹽水等。必要時可適當添加安定化劑、濕潤劑、乳化劑、黏合劑、等張化劑等添加劑。 35 318986 200800289 〜Γί發明之劑或組成物中’選定之化合物之有效投予 m象ΐ年齡、體重、症狀、投予途徑、投予時間二衣鳥恶等適當選擇、決定，例如經口服用時，甜菊投予量較好為每日u5G(K)mg、更好為1G至誦mg。卜為10至2000mg，更好為100至i〇〇〇mgo 该寺亦可1日分數次投予。本發明之劑或組成物中，敎之植物、動物、礦物、予量係根據對象之=二:取二:卒取水解物之有效投牛π肢重、症狀、投予途徑、投予時 2表、製劑形態、材料活性之強度等適當選擇、決定，例如經口服用時’魚腥草萃取物之投予量較好為每日」至 50000mg’更好為1〇至1〇〇〇呢。維生素匸較好為每日 ^_mg，更好為⑽幻〇_g。該等亦可工日分數于0 -之或組成物作為皮膚外用’可作成藥事法所 % 外用醫藥品、醫藥品等製品。可用於如乳液、礼相、化粧水（loti〇n)等皮膚保養製品、軟惟 =於此，其劑型可為水溶液系、可溶化系、乳化系： +乐、油液糸、凝膠系、軟膏系、氣溶膠系、水-油2層系、水-油-粉末3層系等廣範圍劑型。旦本發明之劑或組成物為皮膚外用時，所選定化合物之含置係根據症狀之不同適當選擇，例如為夏 — 約總重量之。.。01至1〇重量％，較好為〇. 〇1至2古重τ量又，維生素C衍生物係使用2-〇-㈣糖基_抗壞丙 318986 36 200800289 酸時，為總重量之0·001至50重量％，較好為〇1至1〇重嚴％。可分成1至4次/日塗抹。 ‘ 本發明之劑或組成物為皮膚外用時，所選定之植物之 • 3里係根據症狀之不同適當選擇，例如為葡萄柚萃取物時為約總重量之（L 〇01至50重量％，較好.為〇〇1至1〇重量 %。又，維生素C衍生物係使用2—0-葡萄吡喃糖基一抗壞丙酸時，為總重量之0.001至50重量％，較好為馨至10重量％。可分成1至4次/日塗抹。以下列舉實施例作具體說明，惟，不只限於此。實施例1 (植物之萃取） · 在厚朴（Mognalia obovata)、扁桃仁（prunus dulcis)、繡球花（Hydrangea macrophylla)、葡萄柚 (Citrus paradise)、龍鬚草（Ophiopogon jponicus)、西洋常春藤（Hedera hel ix)、石驗草（Saponiria 馨 officinal is) ' 魚腥草（Houttuynia cordata)、洋甘菊 (Matricaria chamomilla)、棕兒茶（Uncalia gambir)、金縷梅（Hamamelis virginiana)、桑（Morus alba)、米糠 (Oryza sativa)、問剂（Equisetum arvense)之乾燥物 lOOOg中加入90%乙醇液12L(公升），於4(TC攪拌5小時。經由濾紙回收濾液。反覆操作2次，將濾液以蒸發器減壓濃縮，除去乙醇溶劑。其結果獲得厚朴萃取物120. 6g、扁桃仁萃取物150· 4g、繡球花萃取物170. 9g、葡萄柚萃取物 127. 3g、龍鬚草萃取物115· 3g、西洋常春藤萃取物 3189S6 37 200800289 204.8g、石驗草萃取物斗物143·7§、魚腥草萃取物133.9g'洋 2ni93m茶萃取物168.2g、金縷梅萃取物 ..g木卞取物1〇3.8g、米糠萃取物184.7g、問荊萃取 •物102. 7g之乾燥重量。實施例2 (測疋肝細胞中維生素C運轉體之生產促進活性） ,: '原自人颁肝癌之HepG2細胞（大日本製藥（股）公司春製造）在直經3.—之培養皿（法魯康公司製造）以5χΐ〇3 cel Is接種’在合有1〇%非作用化牛胎兒血清（_)之⑽Em 培養液（吉普可公司製造），於抓、5%二氧化碳氣體存在下培養3日。3日後以新的培養液替代，作為被驗物質添=用細胞。被驗物質夏至草苦素、膽巢基苯曱酸酯、幽卡菲林、拉皮菲林、香葉基乙酸醋、燈花叔醇、二氯莱莉酮、香芹基乙酸酯、愈瘡奠、二氫松柏醇、甜菊糖、_ 蒎烯、馬鞭草苷、丨-肉豆蔻醇、4_經基香豆素、膽酸、膽固醇油酸酯、椁皮苷、佛手酚係由福納可昔公司購入。將被驗物質溶解於二曱亞砜（DMS〇)，添加於被驗物質添加用、、、田胞中並使隶終濃度成為1 Q Q y U，6小時後使用TR I ZQL 試藥（jnvitrogen公司製造），根據常法將所有RNA回收。回收之所有 RNA 使用 First-strandcDNA Synthesis kit(阿瑪謝生物科技公司製造）轉換成cDNA，供即時(rea卜time) PCR。即時 pcR 係根據 QuantiTectSYBR Green PCR Kit(奇阿源公司製造），調製模型，使用即時PCR裝置（M J研究公司製造），測定SVCT1、SVCT2及甘油醛3-磷酸脫氫酶（GAPDH) 318986 38 200800289 mRNA之表現促進活性。已知GAPDH基係管家基因 (housekeeping gene)，由於在所有細胞怪常性表現，而廣，泛被使用作為内部標準之控制基因（contr〇1 gene)。又， <以無添加被驗物質（DMS0 0· 1%)作為對照群。PCR用引子及PCR條件如下所示。 <引子> SVCT1 用 φ 5’-TTC TGA TTGTGC TGC TGACC-3’（序列表序列編號 n 5’-ATG TCA CTGTTG GCA TGAGC-3,（序列表序列編號 2) SVCT2 用 5 -GGA ACC TCTTTG TGC TTGGA-3’（序列表序列編號 3) 5’-CCT GGG ATGGTG TTA TCCAG-3’（序列表序列編號 4) GAPDH 用 5’-CGA CCA CTTTGT CAA GCTCA—3’（序列表序列編號 5) 5 -TTC CTC TTGTGC TCT TGCTG-3’（序列表序列編號 6) ❿ < PCR之反應條件〉關於 SVCT1 及 SVCT2 以（i)95 C、15 分—GOMt：、15 秒—（iii)55t：、30 秒The "vitamin C transporter production promoter" of the invention refers to a person who enhances the expression of any of the vitamin C transporter type 1 (SVCTlht 2 S (SVCT2), in other words, the SLC23A1 or the indicated gene. The "vitamin C absorption promoting composition" of the present invention means a combination of a vitamin C transporter production promoter and at least one or more vitamin c or vitamin C derivatives. A composition for increasing the concentration of vitamin C in the target organ by oral or via smearing of vitamin C or its derivative. The "vitamin C derivative" of the present invention is in addition to 2-〇-a-D-glucopyran. Examples of the glycosyl-anti-propionic acid include ascorbic acid monoalkyl esters such as L-ascorbic acid monostearate, L-ascorbyl monopalmitate, and L-ascorbic acid monooleate, and L-ascorbic acid monophosphate. Ascorbic acid monoester derivative such as L-ascorbic acid-2-sulfuric acid, L-ascorbyl distearate, L-ascorbyl monopalmitate, L-ascorbic acid dioleate, etc. Ascorbic acid dialkyl ester L'-ascorbic acid Filling acid Ascorbic acid di-glycan derivative, l-anti-salt acid distearate, L-ascorbic acid palmitate, L-ascorbic acid 33 33189S6 200800289 Etc., 'L-ascorbic acid triphosphate and other salts of anti-acid and various bases. (4) ' ^Limited to this. Salts can be cited as anti-scurvy · metal salts (example (4) salt, town salt (such as sodium salt), alkaline earth The present invention...the second is not limited to this. The heart-thinking substance with desalination means that the existing liver spots and freckles are excessive, and the fruit is two: the spot and the freckles are melanin (five)-in). It is known that the vitamin C system is the sputum of the sputum sputum +7? The inhibitor of the tyrosinase of the guan guan, pt, ... color male, the phase is made into a reduced light color; Dark melanin liver spots • Freckles, etc., the role of Γ, ί: in the clinical improvement of the existing search; 3W children report (non-皇(四) 2Η7), 725-729) 7: Lin 1967 The vitamins in the skin cells are enhanced by the composition. The whitening effect of the present invention is to use the whitening effect of C, the sputum, the sputum, the sputum, the sputum, the sputum, the sputum, the sputum, the sputum, the sputum, the sputum, the sputum, the As long as it does not inhibit the promotion and action of vitamins, it can also be adjusted by adding other additives. The "winning; & collagen production promotion method of the present invention refers to the function of "promoting J" by the living body, by mentioning the joy. For example, when the collagen-producing cells are skin, :: two = group of sputum, the composition of the gentleman 1 and the enemy's skin moisturity > when the 1 cell is a bone-forming cell, 'is enhanced bone strength = raw 1 Γ (4) The composition for production may be any one as long as it contains the composition for promoting the present invention, and may be added by adding other additives as long as it does not inhibit the effect. The "glucosidase agent" refers to a person who has glucosidase activity by itself or activates glucoamylase activity. It has glucose (10) activity 318986 34 200800289 itself, such as yeast, bifidobacteria, lactic acid bacteria, and can also be derived from Streptococcus, Fusarium, Streptomyces, Rhizopus, Clostridium, Streptococcus - It is a glucosidase of a microorganism, and is not limited thereto. Examples of the activating glucosidase activity include Echinacea purpurea (Echinacea purpurea, Echinacea angustifolia, Echinacea pallida, Echinacea simuiata - Echinacea paradoxa, Echinacea atrorubens) and the like (Patent Document 1: Special Opening 2005- No. 029546), 10 is not limited to this. The glucosidase agent of the present invention can be used by pulverizing the above materials as it is, or by using the extract of the above materials or extracting the hydrolyzate. The agent or composition of the present invention can be used as a food, a pharmaceutical, a cosmetic or the like. The food can be used as a nutritional supplement food, a functional food, a health food, a specific health food, etc. as a food, for example, it can be blended in a fruit juice beverage. For the administration of pharmaceuticals, when the active ingredient is administered orally, it can be used in a non-toxic manner for pharmaceuticals suitable for rectal administration, injection, etc. The carrier is mixed and administered in the form of a conventional pharmaceutical preparation. The form of the preparation may, for example, be a solid preparation such as a powder, a powder, a granule, a tablet or a capsule, a solution such as a solution, a suspension or an emulsion, a freeze-dried preparation or the like, and the preparation may be prepared by a usual method. Examples of the non-toxic carrier for the above pharmaceuticals include glucose, lactose, sucrose, starch, mannitol, dextrin, fatty acid glyceride, polyethylene glycol, hydroxyethylene starch, ethylene glycol, and polyoxyethyl sorbitol. Anhydride fatty acid ester, amino acid, gelatin, albumin, water, physiological saline, and the like. Additives such as a stabilizer, a wetting agent, an emulsifier, a binder, and an isotonic agent may be added as necessary. 35 318986 200800289 ~ Γί In the agent or composition of the invention, the effective selection of the selected compound, such as age, weight, symptoms, route of administration, time of administration, and the like When the stevia is administered in a daily amount of u5G (K) mg, more preferably 1 G to 诵 mg. Bu is 10 to 2000 mg, more preferably 100 to i〇〇〇mgo. The temple can also be administered on a 1 day basis. In the agent or composition of the present invention, the plant, animal, mineral, and dosage of the cockroach are according to the object = two: take two: the effective fertilization of the hydrolysate, the prawn weight, the symptoms, the route of administration, and the administration 2 The table, the form of the preparation, the strength of the activity of the material, and the like are appropriately selected and determined. For example, when administered orally, the dosage of the extract of Houttuynia cordata is preferably daily to 50,000 mg, preferably 1 to 1〇〇〇. It. The vitamin 匸 is preferably ^_mg per day, more preferably (10) illusion _g. These can also be used as a skin for medical use, such as medicines, pharmaceuticals, etc.. It can be used for skin care products such as lotion, courtesy, lotion (loti〇n), etc., and its dosage form can be aqueous solution, solubilized system, emulsified system: + music, oil 糸, gel system A wide range of dosage forms such as ointment, aerosol, water-oil 2-layer, water-oil-powder 3-layer. When the agent or composition of the present invention is for external use on the skin, the selected compound is suitably selected depending on the symptoms, for example, summer to about the total weight. . . . 01 to 1% by weight, preferably 〇. 〇1 to 2 ancient weight τ, vitamin C derivative is 2-〇-(tetra) glycosyl _ anti-bad 318986 36 200800289 acid, the total weight of 0 001 to 50% by weight, preferably 〇1 to 1% by weight. Can be divided into 1 to 4 times / day to apply. When the agent or composition of the present invention is applied to the skin, the selected plant is appropriately selected depending on the symptoms, for example, about grapefruit extract (about 〇01 to 50% by weight, Preferably, it is 〇〇1 to 1% by weight. Further, when the vitamin C derivative is 2-0-glucopyranosyl-anti-propionic acid, it is preferably 0.001 to 50% by weight based on the total weight, preferably Xin to 10% by weight. It can be divided into 1 to 4 times/day. The following examples are given to illustrate, but not limited to. Example 1 (plant extraction) · In Moganlia obovata, almond ( Prunus dulcis), Hydrangea macrophylla, Citrus paradise, Ophiopogon jponicus, Hedera hel ix, Saponiria officinal is Houttuynia Adding 90% of cordata), chamomile (Matricaria chamomilla), brown tea (Uncalia gambir), Hamamelis virginiana, Morus alba, Oryza sativa, Equisetum arvense dry matter 1000g % ethanol solution 12L ( liters), the mixture was stirred for 5 hours. The filtrate was recovered by a filter paper. The filtrate was collected twice. The filtrate was concentrated under reduced pressure in vacuo to remove the solvent. 150·4g, hydrangea extract 170. 9g, grapefruit extract 127. 3g, Eulaliopsis binata extract 115·3g, western ivy extract 3189S6 37 200800289 204.8g, stone test extracts 143·7 §, Houttuynia cordata extract 133.9g' Ocean 2ni93m tea extract 168.2g, witch hazel extract..g wood extract 1〇3.8g, rice bran extract 184.7g, 荆荆提取•物 102. 7g Dry weight. Example 2 (Measurement of production-promoting activity of vitamin C in the liver cells), : 'HepG2 cells originally produced from human liver cancer (made by Dainippon Pharmaceutical Co., Ltd.) in direct transmission 3. Petri dish (manufactured by Farocon) was inoculated with 5χΐ〇3 cel Is '(10)Em culture solution (manufactured by Jeep Co., Ltd.) containing 1% by weight of non-acting bovine fetal serum (_), 5% carbon dioxide gas It is cultured for 3 days in the presence of it. After 3 days, it is replaced with a new medium, and it is used as a substance to be tested. Cell. The test substance Xiazhi grass bitterin, cholestyl benzoate, ce caffeine, riviphenanthine, geranyl acetate vinegar, sedative alcohol, dichlorolevone, carvyl acetate, the more Sore, dihydroconiferol, stevioside, _ decene, verbena, 丨-myristyl alcohol, 4_ basal coumarin, cholic acid, cholesterol oleate, quercetin, bergamot The company was purchased by the company. The test substance was dissolved in disulfoxide (DMS〇), added to the test substance for addition, and the cell was made to have a final concentration of 1 QQ y U, and TR I ZQL was used 6 hours later (jnvitrogen The company manufactures), recovering all RNA according to the usual method. All of the recovered RNA was converted into cDNA using a First-strand cDNA Synthesis kit (manufactured by Amace Biotech Co., Ltd.) for immediate (time) PCR. The real-time pcR was prepared according to the QuantiTectSYBR Green PCR Kit (manufactured by Chiayuan Co., Ltd.), and the SVCT1, SVCT2, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) 318986 38 200800289 mRNA were measured using an instant PCR device (manufactured by MJ Research). The performance promotes activity. The housekeeping gene of the GAPDH family is known to be widely used as an internal standard control gene (contr〇1 gene) because of its ubiquitous performance in all cells. Further, < the test substance was not added as a test substance (DMS0 0.1%). The PCR primers and PCR conditions are as follows. <Introduction> SVCT1 with φ 5'-TTC TGA TTGTGC TGC TGACC-3' (SEQ ID NO: n 5'-ATG TCA CTGTTG GCA TGAGC-3, (SEQ ID NO: 2) SVCT2 with 5-GGA ACC TCTTTG TGC TTGGA-3' (SEQ ID NO: 3) 5'-CCT GGG ATGGTG TTA TCCAG-3' (SEQ ID NO: 4) GAPDH with 5'-CGA CCA CTTTGT CAA GCTCA-3' (SEQ ID NO: 5) 5 -TTC CTC TTGTGC TCT TGCTG-3' (SEQ ID NO: 6) ❿ < Reaction conditions for PCR > About SVCT1 and SVCT2 with (i) 95 C, 15 points - GOMt:, 15 seconds - (iii) 55t: ,30 seconds

—(iv)72t：、30秒為1循環，再將（ii)至（iv)反覆操作35 次後於4°C保存。關於GAPDH 以（i)95 C、15 分〜（^)94^:、15 秒—（ϋ ί)58Χ：、30 秒 —(iv)72C、30秒為1循環，再將（ii)至（iv)反覆操作35 次後於4°C保存。 318986 39 200800289 PCR引子之適當性經由PCR產物之融解曲線及RT-PCR產物之電泳確認。目的基因之表現量係在PCR產物指 .數函數增幅領域之適當處設定閥值（thresho 1 d)，算出從閥值與PCR產物放大曲線之交點獲得之循環數（threshold cycle : Ct值），進行評估。在各被驗物質間表現量之評估係以無添加被驗物質（DMS0 0. 1%)之對照群之SVCT1或 SVCT2之mRM之Ct值除以GAPDH之Ct值之值作為1，與 0 添加被驗物質時之表現量比較，進行研究。結果示於表1 及表2。其結果與對照群相比，可知促進SVCT1及SVCT2雙方表現之化合物為夏至草苦素及苯甲酸膽固醇酯，只促進 SVCT1表現之化合物為甜菊糖、DL-α-蒎稀、馬鞭草苷、 1 -二十七烷醇、4-羥基香豆素、膽酸、膽固醇油酸酯、椁皮苷、佛手酚，只促進SVCT2表現之化合物為幽卡菲林、拉皮菲林、香葉基乙酸酯、橙花叔醇、二氫茉莉酮、香芹 • 基乙酸酯、愈瘡奠、二氫松柏醇。又，嚐試使用已知在骨芽細胞具有促進SVCT2表現作用之得撒米松（dexamethasone)，與本發明化合物之活性進行比較評估，完全未見到得撒米松在肝細胞中有促進 SVCT2表現之作用。 40 318986 200800289 [表1]測定在肝細胞中維生素C轉運體（SVCT1)之生產促進活性被驗物質 SVCT1 / GAPDH (與對照組之比）复至皁苦素 6· 5 本甲酸膽固醇酉旨 9.1 甜莉糖 5· 0 DL- α -蒎烯 3· 9 馬鞭草苷 2. 9 [一二十七烷醇 2· 9 4-羥 ΛΤϊ — 3. 1 膽酸 3. 3 膽固醇油酸醋 3· 9 柊皮苷 5· 7 佛手酚 3· 8 [表2]測定在肝細胞中維生素c轉運體（SVCT2)之生產促進活性被驗物質 SVCT1/GAPDH(與對照組之比）夏至草苦素 4· 3 苯甲酸膽固醇酯 3.2 幽卡菲林 4. 5 拉皮菲林 3.7 香葉基乙酸醋 ____ 2. 6 橙花叙醇 2. 5 一虱東莉酮「 2· 6 香斤基乙酸酯 2. 5 愈瘡奠 3. 0 -一風松柏醇 -~~-- 4· 2 —---—---- 318986 41 200800289 實施例3 (測定在黑色素細胞（menanocyte)中維生素C運轉體（SVCT) /之生產促進活性） v 將源自人類正常黑色素細胞之NHEM細胞（克拉伯公司製造）在直徑3· 5cm之培養皿（法魯康公司製造）接種5x103 cells，在含有特定添加劑之254S培養液（克拉伯公司製造）中，於37°C、5%二氧化碳氣體存在下培養8日。8曰後以 ⑩新的培養液替代’作為被驗物質添加用細胞。被驗物質係使用實施例1萃取之植物、厚朴（Magnol ia obovata)、扁桃仁（Prunus duleis)、繡球花（Hydrangea macrophyl la)、葡萄柚（Citrus paradise)、龍鬚草（Ophiopogon jponicus)、常春藤（Hedera helix)、石鹼草（Saponiria officinalis)、魚腥草（Houttuynia cordata)、洋甘菊 (Matricaria chamomilla)、兒茶（Uncaliagambir)、金縷梅（Hamamelis virginiana)、桑（Morus alba)、米糠（Oryza _ sativa)、問莉（Equisetum arvense)。將被驗物質在 50% 1，3-丁二醇水溶液中再溶解，使成為i%固形分，在被驗物質添加用細胞中添加該溶液，使最終濃度為〇. 5%，6 小時後使用TRIZ0L試藥（Invitrogen公司製造），根據常法將所有RMA回收。評估使用即時PCR之SVCT1、SVCT2 及GAPDH之表現，係以與實施例2相同之評估方法實施。結果不於表3。其結果為與對照群相比，可知由實施例1萃取之植物萃取物促進SVCT2之表現。又，已知在骨芽細胞具有促進 42 338986 200800289 SVCT2表現作用之得擞米松，在黑色素細胞完全無促進 SVCT2表現之作用。又，本實施例使甩之源自人類正常黑 ▼色素細胞之NHEM細胞由於未見到SVCT1之表現，所以不能實施對於SVCT1表現之促進活性之評估。 [表3] 測疋在黑色素細胞中維生素活性 C轉運體（SVCT2)之生產促進 SVCT2/GAPDH (與對照組之比扁桃仁萃取物繡球花萃取物- (iv) 72t:, 30 seconds is 1 cycle, and then (ii) to (iv) are repeatedly operated 35 times and then stored at 4 °C. About GAPDH (i) 95 C, 15 minutes ~ (^) 94^:, 15 seconds - (ϋ ί) 58 Χ:, 30 seconds - (iv) 72C, 30 seconds for 1 cycle, then (ii) to ( Iv) After 35 operations, store at 4 °C. 318986 39 200800289 The suitability of the PCR primer was confirmed by the melting curve of the PCR product and the electrophoresis of the RT-PCR product. The expression amount of the target gene is set at a suitable threshold in the field of amplification of the PCR product index function (thresho 1 d), and the number of cycles (threshold cycle: Ct value) obtained from the intersection of the threshold value and the amplification curve of the PCR product is calculated. to evaluate. The evaluation of the amount of performance between the tested substances was calculated by dividing the Ct value of mRM of SVCT1 or SVCT2 of the control group without added test substance (DMS0 0.1%) by the value of Ct of GAPDH as 1, and adding with 0 The amount of performance of the substance to be tested is compared and studied. The results are shown in Tables 1 and 2. As a result, compared with the control group, it was found that the compound which promotes the expression of both SVCT1 and SVCT2 is Xiazhicao and cholesteryl benzoate, and the compound which promotes only SVCT1 is stevioside, DL-α-蒎, verbin, 1 - octacosanol, 4-hydroxycoumarin, cholic acid, cholesterol oleate, quercetin, bergamot, compounds that only promote the expression of SVCT2 are acekafilin, lapifen, geranyl acetate , nerolidol, dihydrojasmone, parsley, acetate, sputum, dihydroconiferol. Further, attempts to use dexamethasone, which is known to promote SVCT2 expression in bone bud cells, have been evaluated in comparison with the activity of the compound of the present invention, and no effect of dexamethasone on promoting SVCT2 expression in hepatocytes has been observed. . 40 318986 200800289 [Table 1] Determination of the production-promoting activity of vitamin C transporter (SVCT1) in hepatocytes SVCT1 / GAPDH (ratio to control group) Recombination to saponin 6·5 Cholesteric acid cholesterol 9.1 Sweet Lily 5· 0 DL- α-decene 3· 9 verbena 2. 9 [1,27-alkanol 2· 9 4-oxindole — 3. 1 Cholic acid 3. 3 cholesterol oleic acid vinegar 3· 9 quercetin 5· 7 bergamot 3· 8 [Table 2] Determination of the production-promoting activity of vitamin C transporter (SVCT2) in hepatocytes SVCT1/GAPDH (compared with the control group) Summer solstice 4 · 3 cholesteryl benzoate 3.2 卡卡菲林 4. 5 莱皮菲林 3.7 geranyl acetate vinegar ____ 2. 6 orange flower sulphate 2. 5 虱虱酮酮「 2 2 2 2 2 2 5 愈奠 3 3 . 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 SVCT) / Production-promoting activity) v NHEM cells derived from human normal melanocytes (made by Crabbe) in petri dishes of 3·5 cm in diameter (Faro Inoculated with 5x103 cells, cultured in 254S medium (manufactured by KRAB) containing specific additives, cultured for 8 days at 37 ° C in the presence of 5% carbon dioxide gas. After 8 曰, replaced with 10 new culture solutions. The test substance is added to the test substance. The test substance is the plant extracted by the method of Example 1, Magnol ia obovata, Prunus duleis, Hydrangea macrophylla, Citrus paradise, dragon. Ophiopogon jponicus, Hedera helix, Saponiria officinalis, Houttuynia cordata, Matricaria chamomilla, Uncaliagambir, Hamamelis virginiana, Morus alba, Oryza _ sativa, Equisetum arvense. Re-dissolve the test substance in 50% 1,3-butanediol aqueous solution to make i% solids in the test substance. The solution was added to the cells for addition to a final concentration of 5%. After 6 hours, TRIZ0L reagent (manufactured by Invitrogen) was used, and all RMAs were recovered according to the usual method. The performance of SVCT1, SVCT2 and GAPDH using real-time PCR was evaluated and carried out in the same evaluation method as in Example 2. The results are not shown in Table 3. As a result, it was found that the plant extract extracted in Example 1 promoted the expression of SVCT2 as compared with the control group. Further, it is known that the bone bud cells have the effect of promoting the SVCT2 expression of 42 338986 200800289, and the melanocytes have no effect of promoting the expression of SVCT2. Further, in the present example, since NHEM cells derived from human normal black ▼ pigment cells did not show the expression of SVCT1, evaluation of the promoting activity against SVCT1 expression could not be performed. [Table 3] Measurement of vitamin activity in melanocytes Production of C transporter (SVCT2) promoted SVCT2/GAPDH (ratio to control group) Almond extract hydrangea extract

--------- 米糠萃取物 —~~一—— _ 問莉萃取物被驗物質 (實施例1之取物) 厚朴萃取物龍鬚草萃取物 ^春藤萃取物石驗草萃取物魚腥草萃取物取物 … - _ 萃取物梅萃取物實施例4 (黑色素生成抑制試驗） 43 318986 200800289 為評估本發明獲得之植物與維生素c美白作用之併用效果而進行以下試驗，測定黑色素生成抑制活性。生成黑 ♦色素之細胞係使用源自小鼠之B16—F1〇黑色素細胞瘤細 •胞，在48洞盤之各洞接種使成為2xi〇3cells，在使FBS 最終濃度成為10%而添加之含有葡萄糖胺之最少基礎培養液（MEM)培養5日。之後將培養液更換為含有1〇%FBS、余鹼之MEM，作為被驗物質添加用細胞。被驗物質使用將貝施例1獲得之厚朴、扁桃仁、繡球花、葡萄柚、龍鬚草、 =春藤、石鹼草、魚腥草在50% 1，3-丁二醇水溶液中、再溶解，使成為1%固形分者。添加濃度為使被驗物質最終濃度成為〇.5%、維生素C最終濃度成為l〇〇//g/mL地添加 2被驗物質添加用細胞中，培養3日，作為黑色素生成抑平估用忒樣。黑色素量係將細胞以刮刀處理剥離，離心後以十二烷基硫酸鈉(SDS)溶化，測定475mm、260nm 吸光度而求得。黑色素生成抑制率係在475nm、260nm 之吸光度，以添加被驗物質之培養液培養之細胞吸光度作為、S26G，以未添加被驗物質之培養液培養之細胞吸光度作為C475、C26°，根據下述式1算出。該結果示於表4。 f 4所示’可知單獨添加被驗物質時未看到黑色素生成丨效果若同時添加維生素C，則與單獨之維生素c相及^月,、属地抑制黑色素生成。由此明瞭含有該等被驗物質及維生素C之組成物具有黑色素生成抑制機能，且呈有美白作用。 /' 、 [數] 44 318986 200800289 式黑色素生成抑制率（％ ) = 1-[(S475 /S26d)/ (c475 /C26。）]--------- Rice bran extract -~~一—— _ 莉莉提取物物物物物物物物物物物物物物物物物物物物物物物物物物物物物物物物Extract Houttuynia cordata extract extract - - _ extract plum extract Example 4 (melanogenesis inhibition test) 43 318986 200800289 In order to evaluate the combined effect of the plant obtained by the present invention and vitamin C whitening effect, the following test was carried out. Melanogenesis inhibition activity. The cell line which produces the black ♦ pigment is a B16-F1 〇 melanoma cell derived from a mouse, and is inoculated into each hole of the 48-hole plate to become 2 xi 〇 3 cells, and the final concentration of the FBS is 10%. The minimum basal medium (MEM) of glucosamine was cultured for 5 days. Thereafter, the culture solution was replaced with MEM containing 1% by weight of FBS and residual alkali as cells for test substance addition. The test substance used in the 50% 1,3-butanediol aqueous solution of the magnolia, almond, hydrangea, grapefruit, Eulaliopsis, = ivy, stone grass, and houttuynia obtained in Example 1 Re-dissolve to make 1% solids. When the concentration of the test substance is 〇.5%, and the final concentration of the vitamin C is l〇〇//g/mL, the cells for the test substance addition are added, and the cells are cultured for 3 days, and the melanin production is estimated to be flat. Look like this. The amount of melanin was determined by stripping the cells with a spatula, centrifuging, and then dissolving with sodium dodecyl sulfate (SDS) to determine the absorbance at 475 mm and 260 nm. The melanin production inhibition rate is 475 nm and 260 nm, and the absorbance of the cells cultured with the test substance is used as S26G, and the cell absorbance of the culture medium to which the test substance is not added is C475 and C26°, according to the following. Formula 1 is calculated. The results are shown in Table 4. As shown by f4, it was found that no melanin production was observed when the test substance was added alone. If vitamin C was added at the same time, it was inhibited from melanin production by the addition of vitamin C alone. Thus, it is understood that the composition containing the test substance and the vitamin C has a melanin production suppressing function and has a whitening effect. /', [Number] 44 318986 200800289 Formula Melanogenesis inhibition rate (%) = 1-[(S475 /S26d)/ (c475 /C26.)]

xlOO 扁桃仁萃取物繡球花萃取物葡萄柚萃取物龍鬚草萃取物XlOO almond extract hydrangea extract grapefruit extract asparagus extract

[表4 ]黑色素生成抑制★式被驗物質生 5% 1，3-BG) n藤萃取物石驗草萃取物 ___________ 魚腥草萃取物實施例5 (3次元皮膚模型之黑色素生成抑制試驗）為了評估併用本發明所得之葡萄柚萃取物、作為維生素C ,叮生物之2-0-a -D-葡萄吡喃糖基_抗壞血酸（AA2g)& 作為葡糖苷酶活化劑之紫錐花（Echinacea)萃取物之美白作用效果’根據以下之試驗測定黑色素生成抑制活性。評估係使用克拉伯公司販賣，含有源自正常人類里色素細胞之3次元皮膚模型(祖_3〇〇組套)。根據組套之說 318986 45 200800289 明開始培養，每2日更新培養液及被驗物質，培養1〇日。 ίο日後用磷酸鹽緩衝液（PBS)將組織洗淨後以15mL試管，回收，加入蒸餾水1〇〇 A L，進行超音波破碎。另，加入 • Soluene-350(和光純藥公司製造）9〇〇//L，於1〇〇。〇靜置45 分鉍，將組織及黑色素溶化，經由離心分離（1〇, 〇〇叶_， 10分鐘）除去不溶化區分。黑色素之測定係以合成黑色素 (席格瑪阿德利奇公司製造）作成檢量線，由5〇〇随之吸光度算出。被驗物質係使用將實施例丨獲得之葡萄柚在1，3一丁一醇水溶液中再溶解，成為1%固形分（Gr) 、2-0-a -D- 葡〇萄吼喃糖基-抗壞血酸（AA2G)、將紫錐花之葉之乾燥物以 ^0/乙醇水萃取之紫錐花萃取液（咖）（含有u%紫錐花 =取物（固形物）。在3次元皮膚模型之添加濃度以Gr最終 f度為〇.5%或同時AA2G最終濃度為2%或同時Ech為〇.2 加’分別評估單獨、併用時之黑色素生成抑制活性。其結果示於第1圖。如第1圖所示，可知葡萄柚萃取液(G〇、2U =吼喃糖基-抗壞血酸（AA2G)、紫錐花萃取㈣⑻之任二:獨時與無添加之對照群相比，未見到有意義差，若 —㈣，則確認有意義之黑色素生成抑制活性。 (對人類皮膚因善之試驗）如、射糸外線引起之炎症的抑制、色素沉殿改 1 ·被驗者使用健康成人男性10名（27至35歲）之背部進 318986 46 200800289 行實驗 2.檢體使用作為維生素C衍生物之2-0-α-D-葡萄吡喃 .糖基-抗壞血酸（AA2G)及作為維生素C轉運體生產促進劑之葡萄柚萃取物（GFE)，調製如下述表5所示之組成之檢以下，使用下述組成之檢體進行評估試驗。 [表5 ] AA2G GFE 1，3-BG 精製水實施例6 2. 0% 0. 003% 0. 247% 殘餘比較例1 2. 0% 0. 0% 0. 247% 殘餘比較例2 0. 0% 0. 003% 0. 247% 殘餘比較例3 0. 0% 0. 0% 0. 247% 殘餘 AA2G ··林原製造2-0- ck -D-葡萄比鳴糖基-抗壞血酸 GFE: —丸法可斯（PHARC0S)公司製造葡萄柚果實抽提物法可斯雷斯葡萄柚B中之固形分 1，3-BG : 1，3-丁二醇 3.試驗方法 3. 1評估炎症之抑制效果在背部，在每個檢體設定4x4cm 之塗抹領域，塗抹檢體8週。塗抹量以每次3mL、每週約4 次，8週約32次（28至35次）塗抹。8週後测色該部位之表色系之a*值，另照射MED1. 2倍之太陽紫外線，2 曰後測色同部位之a*值。從紫外線照射2日後之a*值扣除紫外線照射前之a*值，求得△ a*值。△ a*值越小則炎症之 47 318986 200800289 抑制效果越高。 3. 2評估色素沉殿之改善效果在背部’在母個檢體設定、4x4cm之塗抹領域，測色檢體塗抹前之L*a*b*表色系之L* 值。接著，照射MED1. 2倍之太陽紫外線，2日後測色同部位之L*值。從2日後之L*值測色後開始塗抹檢體。塗抹量以每次3mL、每週約4次，8週約32次（28至35次）塗抹。紫外線照射2、4、8週後測色同部位之L*值。從紫外線照 0 射2日後及紫外線照射2、4、8週後之L*值扣除檢體塗抹前（紫外線照射前）之L*值，求得AL*值。AL*值之負值越小則色素沉殿越改善。 3. 3測定機器測色：MINOLTA公司製造分光測色計CM-800D 紫外線照射：SOLAR LIGHT公司製造MODEL XPS 200 4. 測定結果 4. 1炎症之抑制效果各檢體塗抹部位之△ a*值示於表 • 6、第2圖。 [表6 ] 檢體 △ a木值實施例6 0. 82 比較例1 1. 33 比較例2 2· 25 比較例3 2· 10 只配合作為維生素C轉運體生產促進劑之PFG之比較 48 318986 200800289 例2之△ a*值與元全不配合之比較例3之值幾乎為同值。因此明瞭只有GFE時未見到抗炎症效果。另一方面，，併用GFE及作為維生素C衍生物之AA2G之實施例6與比較 •例3相比較，顯示有意義之抗炎症效果。因此，實施例6 之Aa*值比只配合AA2G之比較例1之值減低6〇%。由於未見到GFE本身之抗炎症效果，因而將gfe與AA2G 併用之實施例6與只單獨配合AA2G之比較例1相比較，顯 φ 示之優越抗炎症效果為超出預測之顯著效果。 4· 2色素沉澱之改善效果各檢體塗抹部位之ΔΙ^值示於表7、第3圖。 [表7] 檢體 UV 照射前 UV照射 2曰後 UV照射 2週後 UV照射 4週後 UV照射 8週後實施例6 0 - 2· 17 -0· 95 -0· 50 0. 44 比較例1 0 -2· 07 -1· 57 -1· 25 -0. 09 比較例2 0 -2· 06 -2. 23 -1· 75 -0.15 比較例3 0 -2· 02 -1· 98 -1· 92 -0· 99 於紫外線照射2週後只配合維生素C轉運體生產促進劑之GFE之比較例2之Δβ值，其負值較完全未配合之比較例3之△!；*值大。因此明瞭只有GFE時未見到色素沉澱改善效果。另一方面，併用GFE及作為維生素C衍生物之 AA2G之實施例6與比較例3相比，顯示有意義之色素沉澱改善效果。實施例6之△ L*值比只配合AA2G之比較例1 之△ L*值之負值減低60%，與比較例1相比，顯示有意義 318986 49 200800289 之色素沉殿改善效果。由於未見到GFE本身之色素沉殿改善效果，將GFE與AA2G併用之實施例6與只單獨配合ΑΑ% •之比較例1相比’顯示之優越色素沉殿改善效果為超出預 •測之顯著效果。於紫外線照射4週後看到與紫外線照射2 週後幾乎相同之傾向。即使於紫外線照射8週後實施例6 之色素沉殿改善效果亦最優越。以下列舉處方例加以具體說明，惟不只限於此。 φ 實施例7 (測定葡萄柚萃取物對維生素c轉運體（SVCT)之生產促隹活性）、將老鼠黑色素細胞瘤Bi6-F10細胞（大日本製藥（股）公司製造）在直徑3. 5cm之培養皿（法魯康公司製造）以 5xl03celis接種，在含有1〇%非作用化牛胎兒血清（fbs) 之DMEM培養液（吉普可（GIBC0)公司製造），於3rc、5% 二氧化碳氣體存在下培養。培養3曰後以新的培養液替釀代，添加作為被驗物質之葡萄柚（Citrus 萃取物，使在培養基中之最終濃度為〇.6，1δ，6，18,6()，ΐ8()^^ /ml ,6小時佼使用TRIZ0L試樂（Invi trogen公司製造），根據常法將所有RNA回收。添加實施例6使用之一丸法可斯公司製造葡萄柚果實抽提物法可斯雷斯葡萄柚B 作為葡萄柚萃取物。該葡萄柚萃取物係由在葡萄柚ci汁μ paradisi Macfadyen(Rutaceae)之生果實 5kg 中加入 1，3- 丁二醇，浸潰、過濾，在該濾液20kg中加入精製水2〇kg，混合，過濾而獲得者。葡萄柚萃取物之濃度換算為固形物 338986 50 200800289 濃度為葡萄柚萃取液中0.6%(6mg/mL)。回收之所有RNA供給即時定量一步驟（one step)RT-.PCR。根據 QuantiTect SYBR Green PCR Kit(奇阿源（QIAGEN) .公司製造），調製模板（template)，使用PCR裝置（MJ研究公司製造），測定SVCT2及甘油醛3-磷酸脫氫酶（GAPDH) mRNA之表現促進活性。PCR用引子及PCR條件如下所示。 <引子> ⑩ SVCT2用[Table 4] Melanin production inhibition ★ Formula test substance 5% 1,3-BG) n vine extract stone extract ___________ Houttuynia cordata extract Example 5 (3D skin model melanin production inhibition test In order to evaluate and use the grapefruit extract obtained by the present invention, as a vitamin C, 2-0-a-D-glucopyranosyl-ascorbic acid (AA2g) of the cockroach & echinacea as a glucosidase activator (Echinacea) Whitening effect of the extract 'The melanin production inhibitory activity was measured according to the following test. The evaluation was conducted using the Krab company, which contained a 3-dimensional skin model derived from normal human chromosomal cells (Zhu _3 〇〇 set). According to the group set 318986 45 200800289 The culture begins, and the culture medium and the test substance are renewed every 2 days and cultured for 1 day. After ίο, the tissue was washed with phosphate buffered saline (PBS) and recovered in a 15 mL test tube, and distilled water was added for 1 〇〇A L to perform ultrasonication. In addition, add • Soluene-350 (made by Wako Pure Chemical Co., Ltd.) 9〇〇//L, at 1〇〇. After standing for 45 minutes, the tissue and melanin were dissolved, and the insolubilization was removed by centrifugation (1 〇, 〇〇 leaf _, 10 minutes). The measurement of melanin was carried out by using synthetic melanin (manufactured by Sigma Adelic Company) as a calibration curve, and the absorbance was calculated from 5 〇〇. The test substance was re-dissolved in the aqueous solution of 1,3-butanol by using the grapefruit obtained in Example , to become 1% solid (Gr), 2-0-a-D-glucopyranose - Ascorbic acid (AA2G), Echinacea extract (cafe) extracted from the dried echinacea leaves with ^0/ethanol water (containing u% echinacea = extract (solid matter). In 3 dimensional skin The added concentration of the model is determined by the final f degree of Gr being 〇.5% or the final concentration of AA2G is 2% or Ech is 〇.2 plus' separately evaluated the melanin production inhibitory activity when used alone or in combination. The results are shown in Fig. 1. As shown in Fig. 1, it can be seen that grapefruit extract (G〇, 2U = sucrose-ascorbic acid (AA2G), echinacea extract (4) (8) is two: when compared with the control group without added, If it is meaningful, if it is - (4), it will confirm the meaningful melanin production inhibitory activity. (For human skin due to good test) For example, the inhibition of inflammation caused by the external line of the sputum, the pigmentation hall changes 1 · The testee uses healthy adult males 10 (27 to 35 years old) back into 318986 46 200800289 line experiment 2. Sample use as vitamin C Biological 2-0-α-D-glucopyranose, glycosyl-ascorbic acid (AA2G) and grapefruit extract (GFE) as a vitamin C transporter production promoter, prepared as shown in Table 5 below Hereinafter, the evaluation test was carried out using the sample having the following composition. [Table 5] AA2G GFE 1,3-BG purified water Example 6 2. 0% 0. 003% 0. 247% Residual Comparative Example 1 2. 0% 0 0% 0. 247% Residual Comparative Example 2 0. 0% 0. 003% 0. 247% Residual Comparative Example 3 0. 0% 0. 0% 0. 247% Residual AA2G ··Linyuan Manufacturing 2-0- ck -D-glucosyl-glycosyl-ascorbic acid GFE: -Palacecos (PHARC0S) company made grapefruit fruit extracts, the solid part of the koss grapefruit B 1,3-BG: 1,3- Butanediol 3. Test method 3.1 Evaluation of the inhibitory effect of inflammation On the back, in the application area of 4x4 cm for each specimen, the specimen was applied for 8 weeks. The amount of application was 3 mL each time, about 4 times per week, 8 weeks. Apply about 32 times (28 to 35 times). After 8 weeks, the a* value of the color of the part is measured, and the ultraviolet light of MED1. 2 times is irradiated. After 2 曰, the a* value of the same part is measured. After the ultraviolet radiation for 2 days, the a* value is deducted from ultraviolet rays. The value of a* before irradiation is obtained, and the value of △ a* is obtained. The smaller the value of △ a* is, the higher the inhibition effect is. 47 318986 200800289 The improvement effect of the pigmentation hall is evaluated in the back 'in the parent's specimen setting In the 4x4cm smear field, the L* value of the L*a*b* color system before the color test sample is applied. Next, the MED was irradiated twice as much as the sun's ultraviolet rays, and after 2 days, the L* value of the same portion was measured. The sample was applied after the color measurement of the L* value after 2 days. The amount of application was applied at 3 mL each time, about 4 times per week, and about 32 times (28 to 35 times) in 8 weeks. The L* value of the same part of the color was measured after 2, 4, and 8 weeks of ultraviolet irradiation. The L* value after 2 days from UV irradiation and 2, 4, and 8 weeks after UV irradiation was subtracted from the L* value before the sample was applied (before ultraviolet irradiation) to obtain the AL* value. The smaller the negative value of the AL* value, the better the pigmentation hall. 3. 3 measuring machine color measurement: MINOLTA company manufacturing spectrophotometer CM-800D UV irradiation: SOLAR LIGHT company made MODEL XPS 200 4. Measurement results 4.1 Inhibition of inflammation △ a* value of each sample application site In Table 6. 6, Figure 2. [Table 6] Sample △ a wood value Example 6 0. 82 Comparative Example 1 1. 33 Comparative Example 2 2· 25 Comparative Example 3 2· 10 Comparison of PFG only as a vitamin C transporter production promoter 48 318986 200800289 The value of Comparative Example 3 in which the Δ a* value of Example 2 is not matched with the element is almost the same value. Therefore, it is clear that there is no anti-inflammatory effect when only GFE. On the other hand, Example 6 using GFE and AA2G as a vitamin C derivative showed a significant anti-inflammatory effect as compared with Comparative Example 3. Therefore, the Aa* value of Example 6 was reduced by 6% by the value of Comparative Example 1 in which only AA2G was blended. Since the anti-inflammatory effect of GFE itself was not observed, Comparative Example 6 in which gfe was used in combination with AA2G and Comparative Example 1 in which AA2G alone was used alone showed that the superior anti-inflammatory effect indicated by φ was a significant effect beyond the prediction. 4· 2 Improvement effect of pigmentation The ΔΙ value of each sample application site is shown in Table 7 and Figure 3. [Table 7] UV irradiation before specimen UV irradiation 2 UV UV irradiation for 2 weeks, UV irradiation for 4 weeks, UV irradiation for 8 weeks, Example 6 0 - 2· 17 -0· 95 -0 · 50 0. 44 Comparative Example 1 0 -2· 07 -1· 57 -1· 25 -0. 09 Comparative Example 2 0 -2· 06 -2. 23 -1· 75 -0.15 Comparative Example 3 0 -2· 02 -1· 98 -1 · 92 -0· 99 The Δβ value of Comparative Example 2 in which only the GFE of the vitamin C transporter production promoter was added after 2 weeks of ultraviolet irradiation, the negative value was larger than the Δ!* value of Comparative Example 3 which was completely unmatched. Therefore, it is clear that no pigmentation improvement effect is observed when only GFE is used. On the other hand, Example 6 in which GFE and AA2G as a vitamin C derivative were used in combination showed a significant pigment precipitation improving effect as compared with Comparative Example 3. The ΔL* value of Example 6 was reduced by 60% from the negative value of the ΔL* value of Comparative Example 1 in which only AA2G was blended, and compared with Comparative Example 1, the effect of improving the pigmentation of 318986 49 200800289 was exhibited. Since the effect of improving the pigmentation of GFE itself was not observed, the improvement effect of the superior pigmentation hall was better than that of Comparative Example 1 in which GFE and AA2G were used together with Comparative Example 1 alone. Significant effect. After 4 weeks of ultraviolet irradiation, it was almost the same as that after 2 weeks of ultraviolet irradiation. The improvement effect of the pigmentation hall of Example 6 was most excellent even after 8 weeks of ultraviolet irradiation. The following is a list of prescription examples, but is not limited to this. 5厘米的直径。 The ph ph ph ph ph ph ph ph ph ph ph ph ph ph ph ph The culture dish (manufactured by Farocon) was inoculated with 5×10 03 celis in DMEM medium (manufactured by GIBC0) containing 1% by weight of non-acting bovine fetal serum (fbs) in the presence of 3rc, 5% carbon dioxide gas. to cultivate. After culturing for 3 以, the new culture medium was used for the replacement, and the grapefruit (Citrus extract) was added as the test substance so that the final concentration in the medium was 〇.6, 1δ, 6, 18, 6(), ΐ8 ( ^^ /ml, 6 hours, using TRIZ0L test music (manufactured by Invi trogen), all RNA was recovered according to the usual method. Adding Example 6 using one of the pills, the French grapefruit fruit extract, the method Grapefruit B is a grapefruit extract. The grapefruit extract is made by adding 1,3-butanediol to 5kg of the fruit of the grapefruit ci juice μ paradisi Macfadyen (Rutaceae), impregnated and filtered. 2 kg of purified water was added to 20 kg, mixed, and filtered. The concentration of grapefruit extract was converted to solids 338986 50 200800289 The concentration was 0.6% (6 mg/mL) in grapefruit extract. Quantitative one-step RT-.PCR. According to the QuantiTect SYBR Green PCR Kit (manufactured by QIAGEN), a template was prepared, and a SVCT2 and glycerol were measured using a PCR device (manufactured by MJ Research). Aldehyde 3-phosphate dehydrogenase (GAPDH) mRNA Performance-enhancing activity. The PCR primers and PCR conditions are as follows. <Introduction> 10 SVCT2

Mm—Slc23a2_l—SG QuantiTect Primer Assay(奇阿源公司製造） GAPDH 用 5’-AAC TTT GGC ATT GTG GAA GG-3’（序列表序列編號 7) 5’ -ACA CAT TGG GGG TAG GAA CA-3’（序列表序列編號 8) < PCR之反應條件〉以（i)50°C、30 分—（ii)95°C、15 分—、15 秒 • — (iv)55°C、30 秒—（v)72°C、30 秒為 1 循環，再將（ii) 至（v)反覆操作40次後於4°C保存。 PCR引子之適當性係經由PCR產物之融解曲線與RT-PCR產物之電泳確認。目的基因之表現量係在PCR產物指數函數增幅領域之適當處設定閥值，從閥值與PCR產物增幅曲線之交點獲得之循環數（Ct值），以下述之公式算出，表示對内部標準之GAPDH之表現量之數值。 [數] 式2 51 318986 200800289 RNA 表現量 Y=2—(Ct) [數] . 式3 • SVCT2 之表現量=γ(5ΚΤ2)/γ((；Αρΐ)Η) 表現量之比較評估係以無添加被驗物質 SVCT2表現量作為丨，根據相對值進行比較。結果圖。由該結果可知係依存葡萄抽萃取物之濃度而 φ觀2之表現。考慮從PCR原、理上α值之計測值產生之學差’另，確認在實_3，贿產生促進效果為丨4件以上之植物萃取物係在實施例4之黑色素生同時添加維生素C，與單獨之維生素€相比，具有 H 色素抑制效果之作用，因而與對照群相比，對於增加U 倍以上SVCT2表現量者判斷有SVCT促進效果。其結果可知在小鼠黑色素細胞瘤B i 6 一F i 〇細胞中，葡萄抽萃取乩以上有促進SVCT2表現之效果。 _實施例8 (測疋化合物促進維生素c轉運體（SVCT)產生之活性），將源自人類大腸癌之Cac〇2細胞（大曰本製藥（股）公 5丨心）在直從3.5(：111之培養皿以5\103〇€113接種，在含有。1〇%非作用化牛胎兒血清（FBS)之DMEM培養液，於 5 乂一氧化碳氣體存在下培養。培養3日後以新的培 <基曰代，添加作為被驗物質之甜菊糖（CAS : 57817一89 7)、香葉基乙酸酯（CAS : 105-87-3)、橙花叔醇（CAS : 7212-44-4、 _ ^ 、二虱末莉酮（CAS : 1 128-08-01 )、夏至草苦本 52 318986 200800289 (CAS: 465-92-9)、馬鞭草苦（CAS:548_37_8)、轉皮苦（CAs: 524-30-1 )、佛手以⑽：486普2)，使在培養基中之最。終濃度為100/zM。從添加被驗物質6小時後使用triz〇lMm-Slc23a2_l-SG QuantiTect Primer Assay GAPDH with 5'-AAC TTT GGC ATT GTG GAA GG-3' (SEQ ID NO: 7) 5' -ACA CAT TGG GGG TAG GAA CA-3' (Sequence Listing Sequence No. 8) <Reaction conditions for PCR> (i) 50 ° C, 30 minutes - (ii) 95 ° C, 15 minutes -, 15 seconds • (iv) 55 ° C, 30 seconds - (v) 72 ° C, 30 seconds for 1 cycle, and then (ii) to (v) repeated operation 40 times, then stored at 4 ° C. The suitability of the PCR primer is confirmed by electrophoresis of the PCR product and electrophoresis of the RT-PCR product. The expression amount of the target gene is set at a suitable point in the field of amplification of the index function of the PCR product, and the number of cycles (Ct value) obtained from the intersection of the threshold value and the amplification curve of the PCR product is calculated by the following formula, indicating the internal standard The amount of performance of GAPDH. [Number] Equation 2 51 318986 200800289 RNA performance Y=2—(Ct) [Number] . Equation 3 • SVCT2 performance = γ(5ΚΤ2)/γ((;Αρΐ)Η) The comparison of performance is evaluated by The amount of the SVCT2 expression of the test substance was not added as 丨, and the relative value was compared. Result graph. From this result, it is understood that the concentration of 葡萄2 is dependent on the concentration of the grape extract. Consider the difference between the PCR original and the measured value of the alpha value. In addition, it is confirmed that the botanical extract with a bovine production promoting effect of more than 4 is added to the melanin of the fourth embodiment while adding vitamin C. Compared with the vitamin B alone, it has the effect of suppressing the effect of H pigment, so that it is judged to have an SVCT-promoting effect for increasing the amount of SVCT2 expression by more than U times compared with the control group. As a result, it was found that in the mouse melanocytoma B i 6 -F i 〇 cells, the effect of promoting SVCT2 expression was promoted by grape extract extraction. _ Example 8 (Measurement of the activity of the vitamin C transporter (SVCT) by the sputum compound), and the Cac〇2 cells derived from human colorectal cancer (Golden Pharmacy) will be straight from 3.5 ( The dish of :111 was inoculated with 5\103〇€113, and cultured in DMEM containing 1%% non-acting bovine fetal serum (FBS) in the presence of 5 乂 carbon monoxide gas. After 3 days of culture, the new culture was carried out. <Based generation, adding stevioside as a test substance (CAS: 57817-89 7), geranyl acetate (CAS: 105-87-3), nerolidol (CAS: 7212-44- 4, _ ^, diterpene ketone (CAS: 1 128-08-01), Xiazhi grass bitter 52 318986 200800289 (CAS: 465-92-9), verbena bitter (CAS: 548_37_8), skin bitter (CAs: 524-30-1), bergamot (10): 486 pp 2), the most in the medium. The final concentration is 100/zM. After adding the test substance for 6 hours, use triz〇l

.試樂，根據常法將所有腿㈣。回收之所有繼供給即時定量-步驟RT-PCR。根據QuantiTect s繼以⑽pcR (奇阿源公司製造），調製模板，使用p(：R裝置（mj研究公司製造）’測定SVCT1、SVCT2及甘油醛3_磷酸脫氫酶 (GAPDH)m_表現之促進活性。PCR用引子使用與實施例2 使用之相同引子。PCR條件如下所示。 < PCR之反應條件> 数於 SVCT1 及 STCT2 以⑴5(TC、3〇分—（ii)95X、15h(iii)94〇c、i5#、 —(1V)55〇C、30 秒—（v)72t:、3〇秒為 i 循環，再將⑴: 至（v)反覆操作40次後於4。(：保存。. Test music, according to the common law will all legs (four). All subsequent recovery of the supply is immediately quantified - step RT-PCR. According to QuantiTect s, (10) pcR (manufactured by Chiayuan Co., Ltd.) was used to prepare a template, and p(:R device (manufactured by Mj Research Co., Ltd.)' was used to measure SVCT1, SVCT2 and glyceraldehyde 3_phosphate dehydrogenase (GAPDH) m_ The activity was promoted. The primers used for PCR were the same as those used in Example 2. The PCR conditions were as follows: <Reaction conditions for PCR> The number of SVCT1 and STCT2 was (1) 5 (TC, 3 — - (ii) 95X, 15h (iii) 94〇c, i5#, —(1V)55〇C, 30 seconds—(v)72t:, 3〇 seconds is the i cycle, and then (1): to (v) are repeated 40 times and then 4. (:save.

對於GAPDH 以⑴5(rc、30分—（ii)95〇c、15分〜（叫9代、Η秒 —(iv)58t：、30秒—（v)m： ' 3〇秒為i循環，再將⑴: 至（V)反覆操作35次後於4°C保存。 PCR引子之適當性係以PCR產物之融解曲線與產物之電泳確認。以與實施例7相同之算出方法進行目纪基因表現量之比較。結果示於表8。與實施例7相同，以與對照群相比增加1.4倍以上SVCT之表覌量者為|有 STO促進效果’則只促進_表現之化合物為甜菊糖香葉基乙酸酯、二氫茉莉酮、馬鞭草苷、椁皮苷、佛手酚 318986 53 200800289 早苦素。橙花叔醇則促只促進SVCT2表現之化合物為夏至進SVCT1、SVCT2二者之表現。 [表8]For GAPDH, (1) 5 (rc, 30 points - (ii) 95 〇 c, 15 minutes ~ (called 9 generations, leap seconds - (iv) 58t:, 30 seconds - (v) m: ' 3 sec seconds for the i loop, Further, (1): to (V) was repeatedly operated 35 times and then stored at 4 ° C. The appropriateness of the PCR primer was confirmed by the melting curve of the PCR product and the electrophoresis of the product. The same method as in Example 7 was used to carry out the genome gene. The results are shown in Table 8. The results are shown in Table 8. In the same manner as in Example 7, the increase in the amount of SVCT was 1.4 times or more compared with the control group, and the compound having the STO promoting effect was only promoted as the stevia. Geranyl acetate, dihydrojasmone, verbenaside, quercetin, bergamot 318986 53 200800289 early bitterin. nerolidol promotes the compound that promotes SVCT2 only for the summer solstice into SVCT1, SVCT2 Performance. [Table 8]

被驗物質甜菊糖Test substance stevia

香葉基乙酸酯橙花叔醇 f氫茉莉酉同 ΐ至草苦素馬鞭草苷轉皮苦佛手紛實施例9 (使用二次元肝細胞模型測定人 (SVCT)生產之促進活性） '物對維生素C轉運體為了在與生體内相近之頊」用將人類肝細胞三次元培養之^==估，在中空線内使 TESTLIVER、東洋紡社製诰）=' 一-人兀肝細胞模型洞盤中以人類肝細胞無血清謂細胞模型在12 抓，在㈣二氧化肺製造），於行振動培養。振動培養3日^上進為被驗物質之甜菊糖、香。養基s代，添加作莉晒、夏至草苦素、馬鞭草d*橙::醇、二氫茉 ▽皮音、佛手齡，使在培 318986 54 200800289 f基中之最終濃度為⑽―。每24小時更換培養基，每 =4、加被物貝。從添加被驗物質3日後使用TRI胤試 .1·根據系去將所有RNA回收。評估使用即時PCR之 .=二，現係以與實施例8相同之評估方法實施。結禾不於表9。倍以:二?:現1’::照物^ ^ • 表現之化合物里:荦3，促進效果，則只促進 SVCT2表現之化合物為 U曰*馬鞭早苷，只促進表現之化合物為甜菊糖一 1疋、_、SVCT2二者苦、佛手翁。糖、二歧㈣、夏至草苦素、轉皮 [表9]Geranyl acetate, neroli, f-hydrogen, jasmine, sorghum, sorrel, verbena, phorhin, sulphate, sulphate, sulphate, sulphate, sulphate, sulphate, sulphate For the vitamin C transporter, in order to be similar to the human body, the three-dimensional culture of human hepatocytes is used to estimate the TESTLIVER and the Toyobo Co., Ltd. in the hollow line. In the cave plate, a human serum-free cell model of human hepatocytes was grasped at 12, and (di) was produced in a vibrating culture. Vibration culture for 3 days ^Upward The stevia and aroma of the substance to be tested. The nutrient base s generation, added as Li Sun, Xia Zhi grass bitterin, verbena d * orange:: alcohol, dihydro jasmine skin sound, Buddha hand age, so that the final concentration in the cultiture 318986 54 200800289 f base (10) ―. The medium was changed every 24 hours, plus = 4, plus the shell. The TRI test was used 3 days after the addition of the test substance. 1. All RNA was recovered according to the system. The evaluation using the real-time PCR was performed in the same evaluation method as in Example 8. The knot is not in Table 9. Double: 2:: 1':: 照物 ^ ^ • Compounds in the performance: 荦3, promoting effect, the compound that only promotes SVCT2 expression is U曰* 马早 early glycosides, only the compound that promotes performance is stevia One 疋, _, SVCT2 both bitter, bergamot. Sugar, Diqi (four), Xiazhi grass bitterin, peeling [Table 9]

實施例1 〇 (在三次元皮膚模型之色素生成抑制試驗（銀浸染色 318986 55 200800289 (Fontana Masson)染色）在二次兀皮膚模型Me I an〇Derm(克拉柏公司製造估同時添加葡萄柚萃取物及維生素C時之黑色素生成抑制效不以及為了觀祭黑色素細胞形態，以銀浸染色進行黑色；r、之木&又’對於併用具有使α _葡糖苦酶活性上昇、結果示於第萄㈣糖基_抗壞血酸迅速轉換為活性型l 抗壞血酸作用之紫錐花萃取物進行評估。三次元皮膚模型係根據組套說明開始培養，# 2日更換新的培養基及被驗物質’被驗物質使用與實施例5同樣之組合]隹，葡萄袖萃取物使用實施例7使用之萃取物。培養第1()日用顯微鏡硯察形態’用PBS將組織洗淨後根據f法進行銀浸染色。圖該結果可知30#g/mL之葡萄柚萃取物（gfe)、6/zg /虬之紫錐花萃取物（ECE)單獨及兩者之組合，黑色素細月已化准加入2質量％之2-0_ a -D-葡萄吼ϋ南糖基一抗壞血酸（AA2G)，則黑色素細胞收縮、不活性化。又明瞭併用30//g/mL之葡萄柚萃取物（GFE)，可促進黑色素產生抑制活〖生。另明瞭將2質量％之2-0- a -D-葡萄吡喃糖基一抗壞血酸（AA2G)、30//g/mL之葡萄柚萃取物（GFE)、6// g/ mL之紫錐花萃取物（ECE)三種併用，則其黑色素生成抑制活性最高。葡萄柚萃取物（GFE)使用實施例7記载之葡萄柚萃取液予以添加。紫錐花萃取物使用實施例5記載之紫錐花卒取液予以添加。實施例11 318986 56 200800289 (測定維生素c之細胞内吸收量）將小鼠黑色素細胞瘤B16-F10細胞及源自人類大腸癌之Caco2細胞各在直徑i〇cm之培養皿以丨^^^丨^接Example 1 〇 (pigmentation inhibition test in a three-dimensional skin model (silver dip staining 318986 55 200800289 (Fontana Masson) staining) in a secondary sputum skin model Me I an 〇 Derm (Clapberry company estimates and added grapefruit extract Inhibition of melanin production in the case of vitamin C and in order to observe the morphology of melanocytes, black staining with silver staining; r, wood & and 'for the combined use, the activity of α-glucoside enzyme is increased, and the results are shown in The fourth (four) glycosyl-ascorbic acid was rapidly converted to active type l ascorbic acid extract of Echinacea extract. The three-dimensional skin model was started according to the set of instructions, #二日换换新的物质和检测物' was tested The substance was used in the same combination as in Example 5] 隹, the grape sleeve extract was the extract used in Example 7. The culture was observed on the 1st day (1), and the tissue was washed with PBS and then silver stained according to the f method. The results show that 30#g/mL grapefruit extract (gfe), 6/zg / 紫 echinacea extract (ECE) alone and a combination of the two, melanin fine has been standardized 2% by mass of 2-0_ a-D-glucosamine glucosamine-ascorbic acid (AA2G), the melanocytes shrink and inactivate. It is also clear that 30//g/mL grapefruit extract (GFE) is used together. It can promote the inhibition of melanin production. It is also known that 2% by mass of 2-0- a-D-glucopyranosyl-ascorbic acid (AA2G), 30//g/mL grapefruit extract (GFE) The 6/g/mL echinacea extract (ECE) was used in combination, and the melanin production inhibitory activity was the highest. The grapefruit extract (GFE) was added using the grapefruit extract described in Example 7. The flower extract was added using the Echinacea stroke liquid described in Example 5. Example 11 318986 56 200800289 (Measurement of intracellular uptake of vitamin C) Mouse melanoma B16-F10 cells and human colorectal cancer Caco2 cells are each immersed in a dish of diameter i〇cm.

種，在含有10%非作用化牛胎兒血清（FBS)i DMEM培養液，於37°C、5%二氧化碳氣體存在下培養。以黑色素細胞瘤B16-F10細胞培養3日後之細胞、源自人類大腸癌之 Caco2細胞培養1週後之細胞作為維生素c吸收試驗用之細胞。使用實施例7記載之葡萄柚萃取液，添加葡萄柚萃取物使培養液中之最終濃度為3〇//g/mL，以24小時後之細胞用於維生素c吸收試驗。培養液以培養用缓衝液（i5mMThe culture was carried out in the presence of 10% non-acting bovine fetal serum (FBS) i DMEM in the presence of 5% carbon dioxide gas at 37 °C. The cells which were cultured for 3 days after melanoma B16-F10 cells culture, and the cells which were cultured for 1 week from Caco2 cells derived from human colorectal cancer were used as cells for the vitamin C absorption test. Using the grapefruit extract described in Example 7, the grapefruit extract was added so that the final concentration in the culture solution was 3 〇//g/mL, and the cells after 24 hours were used for the vitamin C absorption test. Culture medium to culture buffer (i5mM

Hepes，135mM NaCL，KC1，1. 8mM CaCl2, 0. 8mM MgCi2)洗淨2次後在37 C、5%二氧化碳氣體存在下進行前置培養^ 小時。更換新的培養用緩衝液，添加抗壞血酸（席格瑪3公司製造）作為維生素C並使成為5mM，小鼠黑色素細胞瘤 B16-F10細胞培養3小時、源自人類大腸癌之Cac〇2細胞培養1小時後，以預先冰冷之PBS洗淨3次，將反應停止。在含有lmM EDTA之70%MeOH lml中將抗壞血酸溶出後加入1 OmM二硫蘇糖醇（和光純藥工業（股）公司製造）作為澴原劑，反應10分鐘，轉換為還原型抗壞血酸，以〇 2以/ 過濾器（阿德畔科技（ADVANTEC)公司製造）過濾，濾物用於分析。分析係使用高效液體層析法（jjpLC)，管柱使用卜管柱0DS(4· 6mmx250mm)(化學物質評估研究機構製造）、移動層使用A層（10mM四丁基氫氧化銨溶液/1〇mM磷酸二氫鉀 /0. 5%甲醇PH=6)及B層（50%甲醇）。溶離條件為第〇 318986 57 200800289 至15分鐘只有八層、第15至25鐘係從a層至b層、第 25至35分鐘係從g層至A層之梯度，第35至6〇分鐘只，有A層。在該條件下，還原型抗壞血酸之溶出時間為21 ，分鐘。根據此時波峰之高度，使用檢量線，算出細胞内抗壞金酸濃度，作為維生素C之細胞内吸收量。同時，使用細胞用蛋白質萃取試藥（PIERCE公司製造），從細胞萃取蛋白質。蛋白質定量組套係使用DC protein Assay ⑩（BIO-RAD) ’從根據L〇wry法計測之蛋白質量，以細胞内抗壞血酸吸收量校正，以抗壞血酸量（//m〇1)/蛋白質質量 (mg protein)表示。結果示於第7至1〇圖。該結果可知經由添加葡萄柚萃取物3〇 μ g/mL，細胞内維生素c之吸收量在小鼠黑色素細胞瘤B16-F10細胞增加達約2· 4倍，源自人類大腸癌之Cac〇2細胞增加達約1 · 5倍。實施例12 (葡萄柚萃取物中佛手酚之定量分析）鼸經由HPLC法分析實施例6、7、10、11使用之葡萄柚萃取物（一丸法可斯公司製造葡萄柚果實抽提物法可斯雷斯葡萄柚B中之固形分）中含有之佛手酚濃度。其結果為葡萄柚萃取物中之佛手酚為〇· 〇15質量％。 HPLC分析條件 (官柱：Capcell pak C18 4· 6mm(Dx250mm(SHISEIDO) 管柱溫度：40°C 檢測器：UV 315mm 溶離液：（A)5%乙酸水溶液：（β)乙腈=75 : 25 318986 58 200800289 流速：1 · OmL/分注入量：20/^l 試樣稀釋溶劑：佛手齡溶出時間實施例；13 DMS0/ 乙醇=2 : 18(V/V)。 :9· 6 分 (化合物群之黑色素生成抑制試驗）Hepes, 135 mM NaCL, KC1, 1.8 mM CaCl2, 0.8 mM MgCi2) was washed twice and pre-cultured in the presence of 37 C, 5% carbon dioxide gas for 2 hours. A new culture buffer was added, and ascorbic acid (manufactured by Siegel 3) was added as a vitamin C, and 5 mM, mouse melanoma B16-F10 cells were cultured for 3 hours, and Cac〇2 cells derived from human colorectal cancer were cultured. After 1 hour, the cells were washed three times with pre-ice-cold PBS to stop the reaction. After ascorbic acid was dissolved in 70% MeOH lml containing lmM EDTA, 1 OmM dithiothreitol (manufactured by Wako Pure Chemical Industries, Ltd.) was added as a prionant, and reacted for 10 minutes to be converted into reduced ascorbic acid. 2 Filtered with a / filter (manufactured by ADVANTEC), and the filtrate was used for analysis. The analysis system uses high-performance liquid chromatography (jjpLC), the column is used with the column 0DS (4·6mmx250mm) (manufactured by Chemical Evaluation Research Institute), and the moving layer is used with layer A (10mM tetrabutylammonium hydroxide solution/1〇) mM potassium dihydrogen phosphate / 0. 5% methanol PH = 6) and layer B (50% methanol). The dissolution conditions are No. 318986 57 200800289 to 15 minutes only eight layers, the 15th to 25th clocks from the a layer to the b layer, the 25th to 35th minutes from the g layer to the A layer gradient, the 35th to the 6th minute only There is an A layer. Under this condition, the dissolution time of reduced ascorbic acid was 21 minutes. Based on the height of the peak at this time, the concentration of the intracellular anti-bad gold acid was calculated using the calibration curve as the intracellular absorption of vitamin C. At the same time, the protein was extracted from the cells using a protein extraction reagent (manufactured by PIERCE). The protein quantification kit uses DC protein Assay 10 (BIO-RAD) 'from the amount of protein measured according to the L〇wry method, corrected for intracellular ascorbate absorption, with ascorbic acid (//m〇1) / protein mass (mg Protein). The results are shown in Figures 7 to 1. The results showed that the absorption of intracellular vitamin C increased by about 2.4-fold in mouse melanoma B16-F10 cells by adding grapefruit extract 3〇μg/mL, and Cac〇2 derived from human colorectal cancer. The number of cells increased by about 1.5 times. Example 12 (Quantitative analysis of bergamot in grapefruit extract) 分析 Analysis of grapefruit extracts used in Examples 6, 7, 10, and 11 by HPLC method (Pomegranate extract made from grape pills) The concentration of bergamot contained in the solids of the grapefruit B. As a result, the bergamot in the grapefruit extract was 15% by mass. HPLC analysis conditions (manufactured column: Capcell pak C18 4·6 mm (Dx250mm (SHISEIDO) column temperature: 40 ° C detector: UV 315 mm solution: (A) 5% aqueous acetic acid: (β) acetonitrile = 75: 25 318986 58 200800289 Flow rate: 1 · OmL / min Injection volume: 20 / ^ l Sample dilution solvent: Buddha hand age dissolution time example; 13 DMS0 / ethanol = 2: 18 (V / V). : 9 · 6 points (compound group Melanin production inhibition test)

辛之試驗評估有關本發明所得之化合物與維生 :: 之併用效果，測定黑色素生成抑制活性。源自小鼠B16-F1G黑色素細胞瘤之細胞作為？色二胞，在6洞盤以各洞為1Xl〇5cel 1S接種，以隱 HUm之培養液（吉普可公司製造）培|24小時，使細胞黏著後以__%FBS置換培養液，添加[Nie4，D_phe7]a -MSH(席格瑪阿德利奇公司製造）使成為 ΙΟΟηΜ，以誘導黑色素產生。同時添加被驗物質，幽卡菲林為2 5 # m (含有H /DMS0)、夏至草苦素、拉皮菲林為5〇#m(含有〇. MS〇)、轉皮苦為100#m(含有〇· i%DMS0)，對照組為〇· 1 /6 DMS0。被驗物質單獨群在之後培養48小時，評估黑色素生成抑制。被驗物質與維生素C之併用群在之後放置24 小％ ’添加2次維生素C(席格瑪阿德利奇公司製造）最終濃度4mM(合計添加8mM)，評估黑色素生成抑制。黑色I量之測定係將細胞以胰蛋白酶處理、剝離，以 PBS洗淨反復操作2次，以1 % Tr i tonX(和光純藥公司製造）使細胞可溶化’並測定475nm之吸光度。黑色素量使用合成黑色素（席格瑪阿德利奇公司製造），作成檢量線而算 59 318986 200800289 出。被驗物質與維生素c之併用效果係根據下色素生成抑制率計算。之…、 ‘[式 4] ^ ，L由併用效果之黑色素生成抑制率(％) = ( 併用時之黑色素量/被驗物質單獨時之黑色素量Moo 惟，對照之被驗物質單獨時之黑色素量㈣質用之咖溶劑以最終濃度0.1%添加時之^= ▲ 量，對昭夕输丄古η “、、巴不與維生ic併用時之黑f素量值係指〇.1%_ 主:成抑制率係指相對於單獨添加Q i%刪時之黑色 :里，以併用添加〇1%DMS〇與維為黑色素生成抑制率表示之值。丄里作時，於表10。以°，1細0與維生素c併用添加抑制::;1%DMS0單獨添加時之黑色素量，黑色素生成 >椁皮普與維生素cr卓古素、幽卡菲林、拉皮菲林、 ^ Rq 7 开，則黑色素生成抑制率各分別提高盥維座冬〇 64· 6%、68· 1%、68· 4%。該等為從 0· 1%DMS0 用添加之數值及單獨添加夏至草苦素、幽卡田拉皮非林、椁皮苷之數值所不能預測之顯著相乘效衣0 ^乘效不可推測是由於夏至草苦素、幽卡菲林、拉 =、、椁皮苦之SVCT產生促進效果所致。 L表 10] 夏至草苦素、啦士 # ' 卡非林、拉皮菲林、柊皮苷之黑色素生成 318986 60 200800289 抑制試驗 w 菩、二i王風抑制钕莫被驗物質單獨時' (# g黑色素 /106celIs) -------I· 維生]— 併用時 (# g黑色素 /l〇6celIs) —— 被驗物質經由維生素C 併用效果之黑色素生成抑岳，ί :玄C广叱、對照組 (〇. 1%DMS0) 23· 3 —-—_— 9· 0 4 1 ^ J -ir V To J 61.4 —-— 夏至草苦素 ——— --—--- (50// M) 15. 7 4. 8 69. 7 幽卡菲林 (25/z M) 15. 8 5· 6 64. 6 拉皮菲林 (5£^) 椁皮苷 (100// M) 15. 1 ---—~-— 4.8 —------ 68. 1 22· 2 7· 0 68.4 實施例14 (化合物群之膠原生產評估試驗）為了評估本發明所得之化合物與維生素C之膠原產生能亦稱為㈣合隸進能）之制絲，輯以下之試驗测疋膠原生產促進仙。將源自正f人類皮膚纖維芽細胞 ^ NHDF|B^(Cambrex Bio Science Waklersvi l le ^，1 t 也）在24 >同多盤（法魯康公司製造）以5\1〇4^以/洞接種。’以DMEM+l〇%FBS之培養液（吉普可公司製造），於 37C 一氧化碳氣體存在下培養3日。更換為FBM(serum free)之培養液(三光純藥公司製造)，添加作為被驗物質之 318986 61 200800289 夏至草苦素50 // Μ、甜菊糖、佛手酚各1 〇〇 # μ，對照組為 0· 1% DMSO，進行24小時之前置培養。前置培養後將細胞 ‘以FMB培養液洗淨，更換為FBM培養液後在培養液中添加，維生素C(席格瑪公司製造）使最終濃度成為1〇/ζΜ，處理1 小時使維生素C吸入細胞内。之後將細胞以FMB培養液洗淨，更換為FMB培養液，24小時後對各洞回收imL之調整培養基（conditioned medium(CM))，作為 type 1 collagen _之測定試料。對於上述回收之CM，a typelc〇llagenC_ peptide(PIP)EIA組套（塔卡拉（takara)公司製造）測定膠原置（檢測極限濃度；l〇ng/mL)。在抗PIP單株抗體盤（96 洞）’在各洞加入過氧化酶標識抗ΡίΡ單株抗體1〇〇# 1，接著，每次少量添加預先以含有1%牛血清白蛋白之pBs 稀釋20倍之CM2G#L，於37ec培養3小時。之後倒掉反應液’以200 //L PBS洗淨4次後每次少量添加基質TMBZ (3, 3’，5, 5’ -四甲基聯苯胺）1〇〇/zL，於室溫反應a分鐘。瞻於反應終了加入1N硫酸100/zL，測定波長45〇nm之吸光度。對方；夏至草古素之測定結果不於表1 1。夏至草苦责在併用維生素c時，與對照組比較，確認膠原生產促進作用。甜菊糖、佛手酚之結果示於表12。甜菊糖、佛手酚在併用維生素C時，與對照組比較，確認膠原生產促進作用。 318986 62 200800289 [表 11]The simxin test evaluates the melanin production inhibitory activity by evaluating the effect of the compound obtained by the present invention in combination with vitamins. Is the cell derived from mouse B16-F1G melanoma? The color two cells were inoculated in a 6-hole plate with 1×1〇5cel 1S in each hole, and cultured with cultivar HUM (manufactured by Jeep Co., Ltd.) for 24 hours. After the cells were adhered, the culture medium was replaced with __% FBS and added [ Nie4, D_phe7]a-MSH (manufactured by Sigma Adelic Company) is made into ΙΟΟηΜ to induce melanin production. At the same time, the substance to be tested is added. The cecafilin is 2 5 # m (containing H / DMS0), the summer solstice, the chlorophyllin is 5 〇 #m (containing 〇. MS 〇), and the skin is 100#m ( Contains 〇· i%DMS0) and the control group is 〇· 1 /6 DMS0. The test substance alone was cultured for 48 hours, and the inhibition of melanin production was evaluated. The combined substance and the vitamin C were placed in a small amount of 24% after the addition of 2 times of vitamin C (manufactured by Sigma Adelic Company) to a final concentration of 4 mM (total addition of 8 mM) to evaluate melanin production inhibition. In the measurement of the amount of the black I, the cells were trypsinized and exfoliated, washed twice with PBS, and the cells were solubilized with 1% TritonX (manufactured by Wako Pure Chemical Industries, Ltd.) and the absorbance at 475 nm was measured. The amount of melanin is synthesized using synthetic melanin (made by Sigma Adelic Company) and is calculated as a calibration curve and is calculated as 59 318986 200800289. The combined effect of the test substance and vitamin C is calculated based on the lower pigment production inhibition rate. ..., '[Formula 4] ^ , L is the inhibition rate of the melanin produced by the combined effect (%) = (the amount of melanin used in combination / the amount of melanin when the substance is tested alone, Moo, the melanin of the test substance alone) The quantity (4) of the caffeic solvent used in the final concentration of 0.1% is added as the amount of ^= ▲, and the amount of black 素对、 1 、、、、、、、、、、、、、、、、 1 1 1 1 1 1 1 1 _ Main: Inhibition rate refers to the value of the blackness in the case of adding Q i% alone: in the case of adding 〇 1% DMS 〇 and dimension as the melanin production inhibition rate. With °, 1 fine 0 and vitamin c combined with inhibition::; 1% DMS0 when added alone, the amount of melanin, melanin production > 椁普普 and vitamin cr argusin, 幽卡菲林, 拉皮菲林, ^ Rq 7 On the other hand, the inhibition rate of melanin production was increased by 64.6%, 68. 1%, and 68. 4%, respectively. These are the values added from 0. 1% DMS0 and the addition of summer solacein alone. The value of lycopene and quercetin can not be predicted to significantly multiply the effect of the coat 0 ^ multiplier can not be presumed to be due to the summer solstice卡卡菲林,拉=,,椁皮苦的SVCT production promotion effect. L Table 10] Xiazhi grass bitter, Lai Shi # 'kafilin, lapifen, quercetin melanin production 318986 60 200800289 inhibition Test w Bo, two i king wind suppression 钕 Mo test substance alone ' (# g melanin / 106celIs) ------- I · Weisheng] - when used (# g melanin / l〇6celIs) —— The substance to be tested is produced by vitamin C and the melanin of the effect is used to suppress yue, ί : 玄 C 叱, control group (〇. 1% DMS0) 23· 3 —- — _ — 9· 0 4 1 ^ J -ir V To J 61.4 —-— Xiazhi Phytophthora ——— ------ (50// M) 15. 7 4. 8 69. 7 幽卡菲林(25/z M) 15. 8 5· 6 64. 6 Lapiflin (5£^) quercetin (100//M) 15. 1 ----~-- 4.8 —------ 68. 1 22· 2 7· 0 68.4 Example 14 (Compound Group of collagen production evaluation test) In order to evaluate the production of the compound of the present invention and the collagen production energy of vitamin C, which is also referred to as (4) the synthesis of silk energy, the following test measures the production of collagen by the sputum production. Human skin fibroblasts ^ NHDF|B^(Cambrex Bi o Science Waklersvi l le ^, 1 t also) at 24 > the same multi-disc (made by Farocon) with 5\1〇4^/hole. The culture medium (manufactured by Jeep Co., Ltd.) of DMEM + 10% FBS was cultured for 3 days in the presence of 37C carbon monoxide gas. Replace with the culture medium of FBM (serum free) (manufactured by Sanko Pure Chemical Co., Ltd.), add 318986 61 200800289 as the test substance, Xiazhi grass bitin 50 // Μ, stevia, bergamot 1 〇〇 # μ, control group For 0. 1% DMSO, culture was performed for 24 hours. After pre-culture, the cells were washed with FMB medium, replaced with FBM medium, and added to the culture solution. Vitamin C (manufactured by Siegel) was used to make the final concentration 1 〇/ζΜ, and treated for 1 hour to make vitamin C. Inhaled into the cells. Thereafter, the cells were washed with FMB culture solution, replaced with FMB culture solution, and after 24 hours, imL adjustment medium (CM) was collected from each well to obtain a sample of type 1 collagen. For the above-mentioned recovered CM, a typelc〇llagen C_Peptide (PIP) EIA set (manufactured by Takara Co., Ltd.) was used to measure the gel original (detection limit concentration; l〇ng/mL). In the anti-PIP monoclonal antibody plate (96 holes), add peroxidase-labeled anti-Ρ Ρ Ρ monoclonal antibody 1 〇〇 # 1 in each well. Then, each time a small amount of pre-diluted pBs containing 1% bovine serum albumin was diluted 20 CM2G#L was incubated for 3 hours at 37ec. After that, the reaction solution was poured out. After washing 4 times with 200 // L PBS, a small amount of the substrate TMBZ (3, 3', 5, 5'-tetramethylbenzidine) 1 〇〇 / zL was added each time, and the reaction was carried out at room temperature. a minute. At the end of the reaction, 1N sulfuric acid 100/zL was added, and the absorbance at a wavelength of 45 〇 nm was measured. The other side; the results of the determination of the summer solstice chlorophyll are not shown in Table 11. When the summer solstice grass is used in combination with vitamin C, the collagen production promoting effect is confirmed in comparison with the control group. The results of stevioside and bergamot are shown in Table 12. When stevioside and bergamot were used together with vitamin C, the collagen production-promoting effect was confirmed in comparison with the control group. 318986 62 200800289 [Table 11]

原生產促進作用 77~—-一被驗物時(ng膠原 /10'cells) 維生素C併用時(ng膠原 /105cells) 比率（維生素c 併用時/被驗獨時) 1. 56 對照組 (〇· 1%DMS0) 夏至草苦素 (50// M) --------- 1440 ~ 1~———--- 2242 1510 2567 1. 70 ----—_—— [表 12] 之膠原生產促進作用 (〇· 1%DMS0) Γ ~7·-" ----- 被驗物質單獨時（ng膠原 /105cel Is) 維生素C併用時（ng膠原 /105cel Is) 比率（維生素C 併用時/被驗物質單獨時） 1610 2361 1.47 甜菊糖 (100// Μ) 1553 2724 1. 75 夏至草苦专 (100// 1〇、 1714 2660 1. 55 --- 1 處方例1 [錠劑之製造] 使用魚腥草萃取物及維生素C，根據常法製造下述組成之錠劑。組成魚腥草萃取物 (配合；重量％) 14· 39 14· 39 318986 63 200800289The original production promotion effect 77~—one test substance (ng collagen/10'cells) When vitamin C is used together (ng collagen/105cells) ratio (when vitamin C is used together/tested alone) 1. 56 Control group (〇 · 1%DMS0) Summer solstice (50// M) --------- 1440 ~ 1~————-- 2242 1510 2567 1. 70 -----_—— [Table 12] Collagen production promoting effect (〇·1%DMS0) Γ ~7·-" ----- When the substance is tested alone (ng collagen/105cel Is) When vitamin C is used together (ng collagen/105cel Is) ratio (When vitamin C is used together / when the substance to be tested is alone) 1610 2361 1.47 Stevia (100// Μ) 1553 2724 1. 75 Summer solstice (100// 1〇, 1714 2660 1. 55 --- 1 prescription example 1 [Production of Lozenges] Using Houttuynia cordata Extract and Vitamin C, a lozenge of the following composition is produced according to the usual method. Composition of Houttuynia cordata extract (coordination; wt%) 14· 39 14· 39 318986 63 200800289

橄欖葉萃取水解物 4· 66 胱胺酸 16. 00 於鹼醯胺 1. 2 維生素C 24. 45 纖維素 26. 80 澱粉 4· 00 食用蛋殼粉 6. 00 嚴糖酉旨 2. 50 處方例2 [果汁之製造] 使用含有甜菊糖之阿瑪哈甜菊萃取物及維生素C，根據常法製造下述組成之果汁。組成 (配合；重量％) 果糖葡萄糖液糖 15· 00 檸檬酸 10. 40 維生素C 0. 50 香料 0. 02 色素 0. 10 阿瑪哈甜菊萃取物 10. 00 精製水 63. 98 處方例3 [軟膏之製造] 使用夏至草苦素及維生素C ，根據常法製造下述組成之軟膏。 64 3]8986 200800289 組成 (配合；重量％) 白色凡士林 24. 00 丙二醇 12. 00 硬脂醇 20· 00 對羥基苯曱酸甲酯 0. 05 維生素C 2.00 夏至草苦素 2. 00 香料 0. 10 精製水 39.85 處方例4 [化粧水之製造] 使用葡萄柚萃取物、2-0-α-D-葡萄吡喃糖基-抗壞血酸（AA2G)、紫錐花萃取物，根據常法製造下述組成之化粧水0 組成（配合；重量％) 1，3 - 丁二醇 5. 00 乙醇 4. 50 甘油 10. 00 紫錐花萃取物 0. 50 AA2G 2. 00 葡萄柚萃取物 0. 50 二丙二醇 .2.00 甘草酸二鉀 0. 10 氫氧化鉀 0. 40 65 318986 200800289 精製水 75. 0 處方例5 [片狀面膜（sheet mask)之製造] 使用葡萄袖萃取物、2-0- a -D-葡萄吼喃糖基-抗壞血酸（AA2G)、紫錐花萃取物，根據常法製造下述組成之片狀面膜。組成（配合；重量％ ) 1，3-丁二醇乙醇 6· 00 3. 50 甘油 5. 00 AA2G 2·00 菌類植物膠（Sclerot ium Gum) 0.30 橄欖葉萃取水解物 1.00 紫錐花萃取物 0. 20 檸檬酸鈉 0. 20 • 檸檬酸 0.02 葡萄柚萃取物 0.5 胱胺酸（L08 羧曱基纖維素 0.30 氫氧化鉀 0. 40 精製水 80.50 處方例6 [乳液之製造] 使用葡萄抽萃取物、2 - 0 - α - D -葡萄吼σ南糖基-抗壞血 66 318986 200800289 酸（AA2G)、紫錐花萃取物，根據常法製造下述組成之乳液。組成（配合；重量％)Olive leaf extract hydrolysate 4· 66 cystine acid 16. 00 in alkali decylamine 1. 2 vitamin C 24. 45 cellulose 26. 80 starch 4· 00 edible eggshell powder 6. 00 strict sugar 酉 2. 2. prescription Example 2 [Production of fruit juice] Using the amabi stevia extract containing stevia and vitamin C, a juice of the following composition was produced according to a usual method. Composition (coordination; weight%) fructose glucose liquid sugar 15· 00 citric acid 10. 40 vitamin C 0. 50 fragrance 0. 02 pigment 0. 10 Amaha stevia extract 10. 00 refined water 63. 98 prescription example 3 [ Manufacture of Ointment] Using the summer solstice and vitamin C, an ointment of the following composition is produced according to a conventional method. 64 3]8986 200800289 Composition (coordination; wt%) white petrolatum 24. 00 propylene glycol 12. 00 stearyl alcohol 20· 00 p-hydroxybenzoic acid methyl ester 0. 05 vitamin C 2.00 summer solstice oxalic acid 2. 00 spice 0. 10 Refined water 39.85 Formulation Example 4 [Production of lotion] Grapefruit extract, 2-0-α-D-glucopyranosyl-ascorbic acid (AA2G), and echinacea extract were used, and the following were produced according to the usual method. The composition of the lotion 0 composition (coordination; weight%) 1,3 - butanediol 5. 00 ethanol 4. 50 glycerol 10. 00 Echinacea extract 0. 50 AA2G 2. 00 grapefruit extract 0. 50 two Propylene glycol. 2.00 Dipotassium glycyrrhizinate 0. 10 Potassium hydroxide 0. 40 65 318986 200800289 Refined water 75. 0 Prescription Example 5 [Manufacture of sheet mask] Use grape sleeve extract, 2-0- a - D-glucopyranosyl-ascorbic acid (AA2G), Echinacea extract, a sheet-like mask of the following composition was produced according to a usual method. Composition (coordination; wt%) 1,3-butanediol ethanol 6· 00 3. 50 glycerol 5. 00 AA2G 2·00 Sclerot ium Gum 0.30 Olive leaf extract hydrolysate 1.00 Echinacea extract 0 20 Sodium citrate 0. 20 • Citric acid 0.02 Grapefruit extract 0.5 Cystamine (L08 Carboxymethyl cellulose 0.30 Potassium hydroxide 0. 40 Refined water 80.50 Prescription 6 [Manufacture of lotion] Using grape extract 2 - 0 - α - D - glucosinolate sulphanyl-anti-ascorbic 66 318986 200800289 Acid (AA2G), Echinacea extract, an emulsion of the following composition was prepared according to the usual method. Composition (coordination; wt%)

AA2G 2· 00 氫氧化鉀 0. 38 甘草酸二鉀 0. 10 咕嘲膠 0. 14 產驗桿細屬產生多糖體 0. 07 甘油 2. 00 曱甘醇（methyl glycol) 3· 00 戊二醇 2. 00 f p鱗脂 1· 00 鯊肝醇 0. 10 石夕靈（dimethicone) 1. 50 乙醇 4. 50 紫錐花萃取物 0. 20 荷荷巴葉萃取物 0. 10 橄欖葉萃取水解物 1· 00 葡萄柚萃取物 0. 50 胱胺酸 0. 01 檸檬酸 0. 02 檸檬酸鈉 0. 15 精製水 81. 23 處方例7 [化粧水之製造] 67 318986 200800289 使用香葉基乙酸酯、橙花叔醇、二氫茉莉酮、佛手酚 2-0- a 葡萄吡喃糖基-抗壞血酸<^2(；)、紫錐花萃取物’根據常法製造下述組成之化粧水。 (配合；重量％) 5. 00 4. 50 10. 00 〇. 50 2· 〇〇〇· 01 〇. 01 . 〇. 01 0. 01 2. 00 〇. 10 〇. 40 餘量組成 1，3-丁二醇乙醇甘油AA2G 2· 00 Potassium Hydroxide 0. 38 Dipotassium Glycyrrhizinate 0. 10 咕胶 0 0. 14 The test rod is a genus of polysaccharides 0. 07 glycerol 2. 00 glycolglycol (methyl glycol) 3· 00 戊二Alcohol 2. 00 fp squama 1· 00 Shark liver alcohol 0. 10 Shi Yi Ling (dimethicone) 1. 50 Ethanol 4. 50 Echinacea extract 0. 20 Jojoba leaf extract 0. 10 Olive leaf extract hydrolysis 1· 00 Grapefruit extract 0. 50 Cystamine 0. 01 Citric acid 0. 02 Sodium citrate 0. 15 Refined water 81. 23 Prescription example 7 [Manufacture of lotion] 67 318986 200800289 Use geranyl B Acid ester, nerolidol, dihydrojasmone, bergamot 2-0- a grape pyranosyl-ascorbic acid <^2(;), echinacea extract's make-up according to the usual method water. (fit; weight%) 5. 00 4. 50 10. 00 〇. 50 2· 〇〇〇· 01 〇. 01 . 〇. 01 0. 01 2. 00 〇. 10 〇. 40 balance composition 1,3 -butanediol ethanol glycerol

紫錐花萃取物Echinacea extract

AA2G 香葉基乙酸酯撥Α叙醇 —氣茉莉酮佛手酴二丙二醇甘草酸二钟氫氧化鉀精製水 [產業上利用之可能性]AA2G geranyl acetate, sulphate, jasmonone, bergamot, dipropylene glycol, glycyrrhizic acid, potassium hydroxide, refined water [possibility of industrial use]

胞之：:維生素C轉運體生產促進劑，在使用細現可顯著促進SVCT1及ASVCT2之邏A 利用作為美白用組成物或膠原合成促進【圖式簡單說明】色素生成抑制試驗罘1圖為表示三次元皮膚模型之垔 /\\\ 318986 68 200800289 結果之圖。第2圖為矣一令表不炎症抑制效果之圖。 • 第3圖為矣一、令表不色素沉澱之改善效果之圖。弟4圖為矣一 ‘ 4表7K葡萄柚萃取物之SVa產生促進效果之圖。第5圖為矣一衣7^三次元皮膚模型之顯微鏡照片之圖。第6圖為矣〜不二次元皮膚模型之銀浸染色（Fontana · _ Mason)染色像之圖。第7圖為矣〜衣7κ以HPLC分析抗壞血酸（〇· 5mM)之分析圖表。弟8圖兔本— _ 一、不經由HPLC細胞内抗壞血酸之分析曲線圖〇弟9圖為声-^ 一 # ”、、不在小鼠黑色素細胞瘤B16-F10細胞中，葡萄柚萃取物之 <、、隹生素C吸收促進效果之測定結果之圖。第1 〇圖；4矣-+、響叫— 、不在源自人類大腸癌之Caco2細胞中，葡甸抽萃取物之維生素C吸收促進效果之測定結果之圖。 318986 69 200800289 序列表 <110〉汎蓋爾股份有限公司 <120〉維生素C轉運體生產促進劑 <130> PC27-027 <150 JP 2006-036523 <151>2哪，2,14 <150〉JP 2006-244344 <151>2006-9-8 <150>JP 2006-332214 <151>2006-12-8 <160>8Cell:: Vitamin C transporter production promoter, can significantly promote the use of SVCT1 and ASVCT2 logic A as a whitening composition or collagen synthesis promotion [Simplified illustration] Pigment formation inhibition test 罘 1 The three-dimensional skin model 垔 / \ \ 318986 68 200800289 The picture of the results. Fig. 2 is a graph showing the effect of no inhibition of inflammation on the first order. • Figure 3 is a diagram showing the improvement effect of the non-pigmentation of the table. Figure 4 shows the SVa production-promoting effect of the _4 table 7K grapefruit extract. Figure 5 is a photo of a microscope photograph of a 7^three-dimensional skin model of the 矣一衣. Figure 6 is a diagram of the silver-dipped staining (Fontana · _ Mason) staining image of the 矣~ non-quaternary skin model. Fig. 7 is a chart showing the analysis of ascorbic acid (〇·5 mM) by HPLC.弟8图兔本— _ I. Analysis curve of ascorbic acid in the cells without HPLC. The picture of 〇弟9 is sound-^一# ”, not in mouse melanocytoma B16-F10 cells, < grapefruit extract ;,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, A graph showing the results of the measurement of the effect. 318986 69 200800289 Sequence Listing <110> Pan-Gail Co., Ltd. <120> Vitamin C Transporter Production Accelerator <130> PC27-027 <150 JP 2006-036523 <151>2, 2, 14 < 150> JP 2006-244344 <151>2006-9-8 <150>JP 2006-332214 <151>2006-12-8 <160>8

<210>1 C21D20<210>1 C21D20

<212>DNA <213>Artificiftl Sequence <400>1<212>DNA <213>Artificiftl Sequence <400>1

ttctgattgt gctgctgacc 20 <210>2 <211>20 <212>DNA <213>Artificial Sequence <400>2Ttctgattgt gctgctgacc 20 <210>2 <211>20 <212>DNA <213>Artificial Sequence <400>2

atEtcactft tggc«tg«ic 20 <210>3 〈21]>20 <212>DNAatEtcactft tggc«tg«ic 20 <210>3 <21]>20 <212>DNA

<213>Artific!al Sequence <400>3 ggaacctctt tgi^cttgga 20<213>Artific!al Sequence <400>3 ggaacctctt tgi^cttgga 20

<21 〇M <211>20 <212>0M <213>Art i f i c i a I Sequence <400>4 cctggretgi titutooag 20 70 318986 200800289 <210>δ <211 >20 <212>1脆 <213>Art i f i c i a I Sequence <400>5 csaccacttt gtcaafctod 20<21 〇M <211>20 <212>0M <213>Art ificia I Sequence <400>4 cctggretgi titutooag 20 70 318986 200800289 <210>δ <211 >20 <212>1 Crisp <213>Art ificia I Sequence <400>5 csaccacttt gtcaafctod 20

<210>6 <21V>20 <2i 湖 A <213>Artificial Sequence <400>6 ttcctcttgt gctcttgctg 20<210>6 <21V>20 <2i Lake A <213>Artificial Sequence <400>6 ttcctcttgt gctcttgctg 20

<210>7 <211>20 <212>DNA <2t3>Artificial Scquchcc <400>7 aactttggcft ttft£g88gg 20<210>7 <211>20 <212>DNA<2t3>Artificial Scquchcc <400>7 aactttggcft ttft£g88gg 20

<210>8 <211>20 <212>0NA <213>ArtificiEl Sequence <400>8 acacattggg ggtaggaaca 20<210>8 <211>20 <212>0NA <213>ArtificiEl Sequence <400>8 acacattggg ggtaggaaca 20

71 31898671 318986

Claims

200800289 卜, the scope of application for patents ·· Self-selling body production promoter, characterized by: containing at least linla m grass bitumin, cholesterol benzoic acid vinegar, yoke caffeine bud A heart succinyl acetate vinegar, orange flower Tertiary alcohol, dihydrojasmone, 曰暴乙酸 acetic acid g, Yu test flute, - preparation > Wei welding, verbena bitter, V '17:2: wide, stevia, DL-a one. Alkanol, 4-hydroxycoumarin, cholesteric, 2·

Cholesterol oleic acid, scorpion bitter, sacred hand test compound group: a kind of transporter production promoter, which contains plant, animal, mineral, microbial private solder containing the application of a specific compound, its extract Or a hydrolyzate of the extract. Table C is a composition for absorption promotion, which is a combination of the application of the patent scope 1 or 2 of the vitamin G# (4) production of C or vitamins (: derivative combination. ... 隹 " 4 · If the scope of patent application 3 To sustain the composition for absorption,

- Medium "Hai, the derivative of vitamin C is 2-0-a-D-glucopyranosyl-ascorbic acid. Soil 5 - A composition for Wu Bai, which contains the vitamin C absorption promoting composition of the third or fourth aspect of the patent application. A composition for promoting collagen synthesis, which comprises the composition for promoting vitamin C absorption according to item 3 or item 4 of the patent application. 7. A vitamin C transporter production promoter, characterized in that it contains at least one selected from the group consisting of M〇gnaiia 〇b〇vaia, alum (dulcis), Hydrangeamacrophy 1 la, grapefruit ( Citms paradlse), 龙phi叩〇g〇n jp〇nicus, 318986 72 200800289 Western Ivy (Hedera hel ix), Saponiria of f icinal is, Houttuynia cordata , the crushed material of the group of Matricaria chamomilla, Uncalia gambir, Hamamel is virginiana, Morus alba, Oryza sativa, Equisetum arvense, An extract or a hydrolyzate of the extract. 8. A composition for promoting the absorption of vitamin C, which is characterized in that it is a combination of a vitamin c transporter production promoter of claim 7 and a vitamin 或 or a vitamin C derivative. 9. The vitamin c absorption promoting composition according to item 8 of the patent application, /, the middle name vitamin (: derivative is 2-〇-〇: -1) - grape pyranosyl group - ascorbic acid. A composition for whitening, which is characterized by containing 1 U. - ϊ π π π 祀弟或 or the ninth item of vitamin C absorption promoting composition. The composition 'is characterized by containing the composition for promoting vitamin C absorption of the patent application. The composition of the Wubai composition is characterized by the third aspect of the patent application, the fourth item, the eighth item or the ninth part of the composition for promoting and the whitening composition of the glucose-vitamin C absorption item: , the Portuguese # ^ is at least one type of powder selected from the group consisting of echinacea, yeast cone, and lactic acid bacteria. /, stroke or stroke hydrolysate 318986 73