CN101384241A - Agent for promoting vitamin C transporter production - Google Patents

Abstract

It is intended to provide a substance having an action of promoting vitamin C transporter production. An agent for promoting vitamin C transporter production contains, as an active ingredient, a compound of marrubiin, cholesteryl benzoate, ugaferin, rapiferin, geranyl acetate, nerolidol, dihydrojasmone, carbacryl acetate, guaizulene, dihydroconiferyl alcohol, stevioside, DL-alpha pyrene, verbenalin, 1-heptacosanol, 4-hydroxycoumarin, cholic acid, cholesteryl oleate, fraxin or bergaptol, or an extract of Magnolia obovata, almond, Hydrangea macrophylla, grapefruit, Ophiopogon japonicus, Hedera helix L, Saponaria officinalis L., Houttuynia cordata, chamomile, Uncaria gambir, hamamelis, Morus alba, rice bran or field horsetail.

Description

Agent for promoting vitamin C transporter production

Technical field

The present invention relates to have chemical compound and the plant that vitamin C transporter produces facilitation.In addition, relating to the vitamin C that utilizes this chemical compound and plant absorbs composition for promoting, contains this vitamin C and absorb whitening with compositions or the synthetic composition for promoting of collagen of composition for promoting.

Background technology

Vitamin C has excellent antioxidant effect in vivo as the water solublity antioxidant in the organism to superoxide anion and other active oxygen species, prevents the oxidation of intracellular lipid components.In addition, by controlling the known hydroxylating of the proline residue in synthetic and adjustment that Railway transportation that the tryrosinase of the synthetic rate-limiting enzyme of melanocyte, ascorbic chelation bring send and then regulate oxidation and participate in various enzyme reactions also originally as collagen.Though for having multi-functional vitamin C, but marmot does not have the vitamin C synthesis capability with the primates that comprises the mankind owing to being short of the L-gulonolactone oxidase, and then think vitamin C because have water miscible character, compare the organism retention time with other fatsoluble vitamiies short, thereby causes ascorbic insufficiency of function easily.

As the disease that causes by ascorbic shortage, enumerate the obstacle of reduction, bone and the connective tissue of vitamin C deficiency, wound healing ability, angiokinetic instabilityization etc.And then, vitamin C for comprising various diseases such as heart disease, cancer, cataract, flu as giving anti-or therapeutic purposes are used (non-patent literature 1).In addition, except disease, also provide in a large number skin is whitened and the moist reinforcement of skin and the Vitamin C preparation that aging resistance act as purpose.

On the other hand, although reported opinion about ascorbic function and its effectiveness in a large number, about the absorption principle of vitamin C to each tissue, indefinite point is still a lot.People such as Tsukaguchi had successfully cloned the memebrane protein of bearing ascorbic absorption from rat in 1999, and found the sodium dependency vitamin C transporter (SVCT) (non-patent literature 2) carried in cell with the ratio of sodium ion: vitamin C=2:1.In research thereafter, also there is vitamin C transporter in the clear and definite mankind, in addition, and clear and definite 1 type (SVCT1) and 2 kinds of hypotypes of 2 types (SVCT2) of existing in the vitamin C transporter.SVCT1 mainly is distributed in the epithelial tissue of small intestinal, liver, kidney, large intestine, uterus, prostate etc., and SVCT2 is distributed widely in endothelial tissues such as lung, skeletal muscle meat, eye, bone, brain.Carry vitamin C though be specificity, by dynamic analysis as can be known SVCT2 than SVCT1 specificity excellence (non-patent literature 3).

People such as Alexander have studied the relation of the expression of the vitamin C recoverable amount of following age growth and vitamin C transporter, have reported that the expression of vitamin C transporter (SVCT1) in the rat liver reduces and the minimizing (non-patent literature 4) of vitamin C recoverable amount.As giving of vitamin C recoverable amount reduction method anti-or that improve, enumerated ascorbic continuation picked-up, people such as MacDonald use human gastrointestinal model to infer that superfluous ascorbic picked-up can reduce the expression of vitamin C transporter (SVCT1) and then reduce ascorbic absorption ability (non-patent literature 5).Think that from these opinions agent for promoting vitamin C transporter production is to being effective method about resulting from ascorbic function that the vitamin C recoverable amount reduces reduces give anti-or improve.

So far, few about the report of the chemical compound of the expression that improves SVCT1 and SVCT2 or plant.People such as Fujita have reported the use mouse bone-forming cell, and the expression that dexamethasone (dexamethazone) has improved SVCT2 makes ascorbic intake rising (non-patent literature 6).But dexamethasone is owing to show hormone with effect, thereby from the viewpoint of side effect, is subjected to severely restricts in use.In addition, as yet not relevant for the report of enhanced chemical compound of the expression that makes SVCT1 or plant.

Non-patent literature 1:Molecular Membrane Biology, 2001, Vol.18,87-95

Non-patent literature 2:Nature, 1999, Vol.399,70-75

Non-patent literature 3:FEBS Letter, 1999, Vol.460,480-484

Non-patent literature 4:Archives of Biochemistry and Biophysics, 2003, Vol.410,112-120

Non-patent literature 5:British Journal of Nutrition, 2002, Vol.87,97-100

Non-patent literature 6:British Journal of Nutrition, 2001, Vol.86,145-149

Summary of the invention

The problem that invention will solve

Problem of the present invention is to provide the agent for promoting vitamin C transporter production that promotes that vitamin C is taken in to destination organization.And then another problem contains the whitening with the synthetic composition for promoting of compositions or collagen of such promoter for providing.

The method of dealing with problems

The inventor etc. further investigate in order to solve above-mentioned each problem, found that having vitamin C transporter produces the chemical compound of facilitation and plant etc., it is exceedingly useful absorbing composition for promoting, whiten with compositions, the synthetic composition for promoting of collagen as vitamin C, and from then on viewpoint is set out until having finished the present invention.That is, the solution that is proposed here is following scheme.

(1) agent for promoting vitamin C transporter production, it is characterized in that, contain more than at least one that is selected from the chemical compound group that marrubilin, cholesteryl benzoate, ugaferine, lapiferine, geranyl acetate, nerolidol, dihydro jasmone, Carvacryl acetate (Carvacryl acetate), Kessazulen, dihydroconiferyl alcohol, stevioside, DL-australene, verbenalin, 1-heptacosanol, 4 hydroxy coumarin, cholic acid, cholesteryl oleate, fraxin, bergaptol form.

(2) agent for promoting vitamin C transporter production, it comprises the hydrolysate of ground product, its extract or the extract of the plant that contains aforementioned (1) described arbitrary chemical compound, animal, mineral, microorganism.

(3) vitamin C absorbs composition for promoting, and it combines aforementioned (1) or (2) described agent for promoting vitamin C transporter production and vitamin C or vitamin C derivatives.

(4) aforementioned (3) described vitamin C absorbs composition for promoting, and vitamin C derivatives is 2-O-α-D-glycopyranosyl-ascorbic acid.

(5) whiten and use compositions, it comprises aforementioned (3) or (4) described vitamin C and absorbs composition for promoting.

(6) the synthetic composition for promoting of collagen, it comprises aforementioned (3) or (4) described vitamin C and absorbs composition for promoting.

(7) agent for promoting vitamin C transporter production, it is characterized in that, contain the hydrolysate of above ground product, its extract or extract of at least one that is selected from the group that somewhat-white magnolia Drymotaenium miyoshianum (Mak.) Mak. (Japanese big-leaf magnolia), Semen Armeniacae Amarum, sweet tea, grapefruit, Radix Ophiopogonis, Caulis Hederae Sinensis, Saponaria officinalis, Herba Houttuyniae, Flos Matricariae chamomillae, gambir, Radix Hamamelidis Mollis, Mulberry, Testa oryzae, Herba Equiseti Arvinsis form.

(8) vitamin C absorbs composition for promoting, and it combines aforementioned (7) described agent for promoting vitamin C transporter production and vitamin C or vitamin C derivatives.

(9) aforementioned (8) described vitamin C absorbs composition for promoting, and vitamin C derivatives is 2-O-α-D-glycopyranosyl-ascorbic acid.

(10) whiten and use compositions, it is characterized in that, it comprises aforementioned (8) or (9) described vitamin C and absorbs composition for promoting.

(11) the synthetic composition for promoting of collagen is characterized in that, comprises the described vitamin C in aforementioned (8) or (9) and absorbs composition for promoting.

(12) whiten and use compositions, it is characterized in that, contain aforementioned (3), (4), (8) or (9) described vitamin C absorbs composition for promoting and glucosidase agent.

(13) aforementioned (12) described whitening used compositions, and the glucosidase agent is to be selected from least one in the group that Echinacea Species, yeast, bacillus bifidus, lactobacillus form above ground product, its extract or to extract hydrolysate.

The invention effect

Comprise specific chemical compound and the agent for promoting vitamin C transporter production of plant according to what find, in the analysis of having used cell, can confirm significantly to have promoted the expression of the mRNA of SVCT1 and/or SVCT2 by the present invention.Agent for promoting vitamin C transporter production of the present invention can be used as to whiten and uses with compositions, the synthetic composition for promoting of collagen, uses form as it, is enumerated as per os usefulness, external preparation for skin, injection, cosmetics, medicine etc.

Description of drawings

Fig. 1 generates the result's of inhibition test chart for the melanocyte that shows three-dimensional skin model.

Fig. 2 is the chart of the inhibition effect of demonstration inflammation.

Fig. 3 is for showing the chart that improves effect of pigmentation.

Fig. 4 produces the figure of facilitation effect for the SVCT that shows grapefruit extract.

Fig. 5 is the figure of the microphotograph of the three-dimensional skin model of demonstration.

Fig. 6 is the figure of the Fontana-Masson colored graph picture of the three-dimensional skin model of demonstration.

Fig. 7 is the figure of the analysis diagram of the ascorbic acid (0.5mM) of demonstration use HPLC.

Fig. 8 is the figure of the analysis diagram of the intracellular ascorbic acid of demonstration use HPLC.

Fig. 9 absorbs the figure of the measurement result of facilitation effect for the vitamin C that shows the grapefruit extract in the murine melanoma B16-F10 cell.

Figure 10 absorbs the figure of the measurement result of facilitation effect for the vitamin C that shows the grapefruit extract in the Caco2 cell that is derived from human colorectal cancer.

The specific embodiment

Below, describe the present invention in detail.As the contained chemical compound of agent for promoting vitamin C transporter production of the present invention, can use marrubilin, cholesteryl benzoate, ugaferine, lapiferine, geranyl acetate, nerolidol, dihydro jasmone, Carvacryl acetate, Kessazulen, dihydroconiferyl alcohol, stevioside, DL-australene, verbenalin, 1-heptacosanol, 4 hydroxy coumarin, cholic acid, cholesteryl oleate, fraxin, bergaptol etc.These chemical compounds can use a kind separately, in addition, and use more than 2 kinds also capable of being combined.In addition, also can use ground product, its extract of the plant that contains these chemical compounds, animal, mineral, microorganism or extract hydrolysate.

As the plant that contains marrubilin (CAS:465-92-9), list the Marrubium acetabulosum of Folium Perillae section Marrubium, Marrubium adfine, Marrubium aellenii, Marrubium affine, Marrubium africanum, Marrubium album, Marrubium alternidens, Marrubium alyssoides, front yard mustard marrubium (Marrubium alysson), Marrubium angustifolium, Marrubium anisodon, Marrubium apulum, Marrubium aquaticum, Marrubium aschersoni, Marrubium astracanicum, Marrubiumatlanticum, Marrubium ayardii, Marrubium ballota, Marrubiumballotaeforme, Marrubium ballotoides, Marrubium bastetanum, Marrubium bornmuelleri, Marru biumbourgaei, Marrubiumbrachyodon, Marrubium candidissimum, Marrubiumcatariaefolium, Marrubium cephalanthum, Marrubium cinerascens, Marrubium cinereum, Marrubium circinnatum, Marrubium civice, Marrubium coerulescens, Marrubium condensatum, Marrubiumcordatum, Marrubium crassidens, Marrubium creticum, Marrubium crispum, Marrumbium cuneatum, Marrubiumcylleneum, Marrubium depauperatum, Marrubium deserti, Marrubium duabense, Marrubium echinatum, Marrubiumeriocephalum, Marrubium eriostachyum, Marrubium faucidens, Marrubium flavum, Marrubium flexuosum, Marrubium fontianum, Marrubium friwaldskyanum, Marrubium gamodon, Marrubiumgermanicum, Marrubium glechomaefolium, Marrubium globosum, Marrubium goktschaicum, Marrubium guilliermondii, Marrubiumhamatum, Marrubium hermonis, Marrubium heterocladum, Marrubium heterodon, Marrubium hierapolitanum, Marrubiumhirsutum, Marrubium hispanicum, Marrubium humbertii, Marrubium humile, Marrubium hyperleucum, Marrubium incanum, Marrubium incisum, Marrubium indicum, Marrubium kotschyi, Marrubiumkur dicum, Marrubium kusnezowii, Marrubiumlamioides, Marrubium lanatum, Marrubium laricum, Marrubiumlaurifolium, Marrubium leonuroides, Marrubium libanoticum, Marrubium litardierei, Marrubium lutescens, Marrubiummacrodon, Marrubium malcolmianum, Marrubium marrubiastrum, Marrubium micranthum, Marrubium microphyllum, Marrubiummollissimum, Marrubium montenegrinum, Marrubiummultibracteatum, Marrubium nanum, Marrubium nigrum, Marrubium noeanum, Marrubium odoratissimum, Marrubiumorientale, Marrubium pallidum, Marrubium paniculatum, Marrubium pannonicum, Marrubium parnassicum, Marrubiumparviflorum, Marrubium pauciflorum, Marrubium peregrinum, Marrubium persicum, Marrubium pestalozzae, Marrubiumplicatum, Marrubium plumosum, Marrubium polyodon, Marrubium praecox, Marrubium procerum, Marrubiumpropinquum, Marrubium pseudo-alyssum, Marrubium pseudo-dictamnus, Marrubium purpureum, Marrubium radiatum, Marrubium remotum, Marrubium rotundifolium, Marrubiumrubrum, Marrubium rugosum, Marrubium scrophulariaefolium, Marrubium segobricense, Marrubium sericeum, Marrubiumsetaceum, Marrubium sewerzowi, Marrubium suffuticosum, Marrubium supinum, Marrubium thessalum, Marrubium thouini, Marrubium trachyticum, Marrubium turkeviczii, Marrubiumuncinatum, Marrubium undulalum, Marrubium vaillantii, Marrubium vanense, Marrubium velutinum, Marrubium virescens, Marrubium vulcanicum, Hoarhound. or another name marrubium (Marrmbiumvulgare), Marrubium werneri, Marrubium wilkommi, the leonurus cardiaca of Folium Perillae section mother wort (Leonurus cardiaca), the lion ear flower (Leonotis leonurus) of Folium Perillae section Leonotis etc.

As the animal that contains cholesteryl benzoate (CAS:604-32-0), enumerate syngnathous Solenognathus (Syngnathus acus) etc.

As the plant that contains ugaferine (CAS:63026-58-4), enumerate Ferula arrigonii, grass of Resina Ferulae (Ferula assafoetida), Fructus Foeniculi (Ferula communis), Ferula involucrata, Ferula kuhistanica, Ferula lapidosa, Ferula latipinna, Ferula leucographa, woods gram Resina Ferulae (Ferulalin kii), Ferula sinaica, accurate Ge Er Resina Ferulae (Ferulasoongarcia), Ferula tenuiesta, the Ferula ugamica etc. of Umbelliferae Foeniculum.As the plant that contains lapiferine (CAS:86992-41-8), enumerate Ferula arrigonii, grass of Resina Ferulae (Ferula assafoetida), Fructus Foeniculi (Ferula communis), Ferula involucrata, Ferula kuhistanica, Ferula lapidosa, Ferula latipinna, Ferula leucographa, woods gram Resina Ferulae (Ferula linkii), Ferula sinaica, accurate Ge Er Resina Ferulae (Ferulasoongarcia), Ferula tenuiesta, the Ferula ugamica etc. of Umbelliferae Foeniculum.

As the plant that contains geranyl acetate (CAS:105-87-3), enumerate the Ma Shi fir (Abies mariesii) of Pinaceae Abies, the Ageratum houstonianum Miller. of Compositae ageratum (Ageratum houstonianum), the Rhizoma Atractylodis (Atractylodeslancea) that the Compositae Rhizoma Atractylodis belongs to, the water wally tree (Baccharis dracunculifolia) of Compositae Baccharis, Baccharis latifolia, Baccharis salicifolia, the Bellis perennis (Bellis perennis) that the Compositae Bellis perennis belongs to, the Calendula officinalis of Compositae Calendula (Calendulaofficinalis), the caraway of Umbelliferae Carum (Carum carvi), the Caulerpa racemosa (Forssk) Web V. Bos (Caulerpa racemosa) that the Caulerpaceae Caulerpa belongs to, the Castrum nocturum L of Solanaceae Cestrum (Cestrum nocturnum), the Radix Changii (Changiumsmyrnioides) that the Umbelliferae Radix Changii belongs to, the LAIMENG of Citrus section Citrus (Citrus aurantifolia), Citrus aurantium Linn. (Citrus aurantium), Fructus Citri Sarcodactylis (Citrus bergamia), pomelo (Citrusgrandis), eight the first day of the lunar month (Citrus hassaku), she gives Citrus chachiensis Hort. (Citrus iyo), fragrant citrus (Citrus junos), discipline state Citrus (Citrus kinokuni), Fructus Citri Limoniae (Citrus limon), Fructus Citri (Citrus medica), Xia Cheng (Citrus natsudaidai), grapefruit (Citrusparadise), early red (Citrus reticulate), Fructus Citri sinensis (Citrus sinensis), bitter orange (Citrus sphaerocarpa), acid Fructus Citri tangerinae (Citrus sudachi), three essentials-essence Citrus chachiensis Hort. (Citrussulcata), upright flower Fructus Citri tangerinae (Citrus tachibana), Wenzhou Citrus (Citrus unshiu), the fur pine algae (Codium tomentosum) of Codium fragile (Sur.) Har. section Glyptostrobus, the Cuminum cyminum L of Umbelliferae Fructus Cumini Cymini apium (Cuminum cyminum), the Cymbopogon caesius (Nees) Stapf of grass family Cymbopogon (Cymbopogon caesius), lemon grass (Cymbopogon citratus) (Cymbopogon citratus), block western Herba Cymbopogonis Citrari (Cymbopogon khasianus), citronella (Cymbopogon winterianus), the Daucus carota of Umbelliferae Daucus, the Dracocephalum kotschyi of the Dracocephalum moldabium genus of Folium Perillae section, the Eucalyptus urophylla (Eucalyptusurophylla) that the Myrtaceae Eucalyptus belongs to, the Helichrysum of Compositae Helichrysum (Helichrysum bracteatum), the Herba Houttuyniae (Houttuynia cordata) that Herba Houttuyniae section Herba Houttuyniae belongs to, the Tibetan anise of Winteraceae Illicium (Illicium griffithii), the western regions of the Yunnan Province anise (Illicium merrillianum), the Juniperus oxycedrus of Cupressaceae Juniperus Linn. (Juniperus formosana), Juniperus rigida Sieb.et Zucc. (Juniperusrigida), the Leptospermum javanicum that Myrtaceae Australia Camellia sinensis belongs to, the Ligusticum mutellina of Umbelliferae Ligustrum, the Zimbabwe of Verenaceae Lippia (Lippia javanica), the marrubium (Marrubiumvulgare) of Folium Perillae section Marrubium, the roundleaf Adeps Bovis seu Bubali of Folium Perillae section Origanum (Origanum rotundifolium), the Pelargonium gravelens (Pelargonium graveolens) of cattle Seedling section Pelargonium, the Brazilian Lignum nanmu of nanmu section Taiwan Machilus (Phoebe porosa), the primrose Cuculus polioephalus of Ericaceae Rhododendron (Rhododendron primulaeflorum), the moss rose of Rosaceae Rosa (Rosa centifolia), the Salvia clevelandii of Folium Perillae section Salvia, Salvia fructicosa, Fructus Ananadis comosi Salvia japonica Thunb. (Salvia officinalis), Salvia Sclare L. (Salviasclarea), the Herba Tagetis Patulae of Compositae Tagetes (Tagetes erecta), the Terminalia bentzoe that the Combretum Racemosum Terminalia catappa belongs to, the thuja koraiensis nakai of Cupressaceae Thuja-Arten (Thujakoraiensis), the Thymus sipyleus of Folium Perillae section Thymus, Thymusthracicus, Herba thymi vulgaris (Thymus vulgaris), Herba thymi vulgaris (Thymus zygis), the phoenix green pepper of Rutaceae Zanthoxylum (Zanthoxylum bungeanum), wing handle Pericarpium Zanthoxyli (Zanthoxylum schinifolium) etc.

As the plant that contains nerolidol (CAS:7212-44-4), enumerate the high mountain Millefolium (Achillea alpine) of Compositae Compositae Achillea, Hemerocallis citrina Baroni achillea millefolium (Achilleafilipendulina), Achillea ligustica, Chiba achillea millefolium (Achilleamilleifolium), Achillea moschata, goose-tongue (Achillea ptarmica), hair leaf grass over sixty years of age (Achillea tomentosa), the Ageratum conyzoides of Compositae ageratum (Ageratum conyzoides), pale reddish brown Ageratum conyzoides (Ageratum houstonianum), the Fructus Amomi subulati of Zingiberaceae Amomum (Amomum aromaticum), Fructus Amomi Rotundus (Amomumcardamomum), Fructus Amomi subulati (Amomum subulatum), sand (Amomumxanthioides) contracts, Rhizoma Zingiberis Recens (Amomum zingiber), the false indigo (Amorpha fruticosa) that the pulse family false indigo belongs to, the tea of Theaceae Camellia (Camellia sinensis), the white bean of pulse family Canavalia (Canavalia ensiformis), Semen Canavaliae (Canavaliagladiata), bay bean (Canavalia maritima), the yellow bottle flower (Cestrum aurantiacum) of Solanaceae Cestrum, Radix Oenotherae erythrosepalae (Cestrum nocturnum), fragrance Cestrum purpureum (Cestrumpurpureum), the LAIMENG of Citrus section Citrus (Citrus aurantifolia), Citrus aurantium Linn. (Citrus aurantium), Fructus Citri Sarcodactylis (Citrusbergamia), pomelo (Citrus grarndis), eight the first day of the lunar month (Citrus hassaku), she gives Citrus chachiensis Hort. (Citrus iyo), Fructus Citri grandis (Citrus junos), breast Fructus Citri tangerinae (Citrus kinokuni), Fructus Citri Limoniae (Citrus limon), Fructus Citri (Citrus medica), Xia Cheng (Citrusnatsudaidai), grapefruit (Citrus paradise), early red (Citrus reticulate), Fructus Citri sinensis (Citrus sinensis), bitter orange (Citrus sphaerocarpa), acid Fructus Citri tangerinae (Citrussudachi), three essentials-essence Citrus chachiensis Hort. (Citrus sulcata), upright flower Fructus Citri tangerinae (Citrus tachibana), Wenzhou Citrus (Citrus unshiu), the Croton aubrevillei that Euphorbiaceae Fructus Crotonis belongs to, cascarilla (Croton eluteria), red bud Mongolian oak (Croton japonicum), Nanjing Ramulus et Folium Cotini Coggygiae (Croton sebiferus), Fructus Crotonis (Croton tiglium), Crotonzambesicus, Umbelliferae Panax's Radix Ginseng (Daucus carota), the Durio Zibethinus murr of Bombacaceae durian genus (Durio zibethinus), the Herba Moslae (Elsholtziaciliata) that Folium Perillae section Herba Moslae belongs to, wild bundle (Elsholtzia rugulosa), the Arillus Longan of Sapindaceae Euphoria (Euphoria longan), the Semen Ginkgo of Ginkgoaceae Ginkgo (Ginkgo biloba), the Western Juniperus rigida Sieb.et Zucc. of Cupressaceae Juniperus Linn. (Juniperus communis), Juniperus conferta (Juniperus conferta), Juniperus formosana (Juniperus formosana), Juniperus rigida Sieb.et Zucc. (Juniperus rigida), the Ligusticum mutellina of Umbelliferae Ligustrum, the Cortex Acanthopanacis Radicis branch of Verenaceae Lippia (Lippia alba), Fructus Citri Limoniae crosses river rattan (Lippiacitriodora), Zimbabwe (Lippia javanica), cross river rattan (Lippia nodiflora), Lippia maltuflora, the water mint of Folium Perillae section Mentha (Mentha aquatica), Herba Menthae (Mentha arvensis), Herba Menthae (Mentha canadensis) is produced in Yunnan, lavender (Mentha longifolia), Mentha arvensis L. syn.M.haplocalyxBrig (Mentha piperita), Mentha pulegium Linn. (Mentha pulegium), Herba Menthae Rotundifoliae (Mentha spicata), the Micromeria carminea that Folium Perillae section Herba Micromeriae Biflorae belongs to, the spiced olive (Mimusopselengi) that Sapotaceae bullet wood belongs to, the Herba Ocimi (Herba Ocimi Pilosi) (Ocimum basilicum) that the Folium Perillae Corol belongs to, Ocimumkilimandoscharicum, coronule smokes (Ocimum tenuiflorum), the Herba Oenanthes Javanicae of Umbelliferae Oenanthe (Oenanthe javanica), goat encyclopaedia goat hundred belongs to (Pandanusboninensis), Pandanus tectorius (Pandanus tectorius), the tree Fructus Piperis of Piperaceae Piper (Piper aduncum), betel leaf (Piperbetle), Radix seu Caulis fici Martinii (Piper kadsura) Radix Piperis sarmentosi (Herba Piperis Sarmentosi) (Piper longum), piper methysticum (Piper methysticum), Fructus Piperis (Pipernigrum), false Piper longum (Piper retrofractum), the Herba Portulacae Grandiflorae of Portulacaceae Portulaca (Portulaca grandiflora), Herba Portulacae (Portulaca oleracea), the Salvia clevelandii of Folium Perillae section Salvia, purple back of the body Salvia japonica Thunb. (Salviacryptantha), Salvia fructicosa, Fructus Ananadis comosi Salvia japonica Thunb. (Salvia officinalis), Salvia Sclare L. (Salvia sclarea), and as plant of the raw material of quintessence oil etc.

As the plant that contains dihydro jasmone (CAS:1128-08-01), enumerate the LAIMENG (Citrus aurantifolia) of Citrus section Citrus, Citrus aurantium Linn. (Citrusaurantium), Fructus Citri Sarcodactylis (Citrus bergamia), pomelo (Cirus grandis), eight the first day of the lunar month (Citrus hassaku), she gives Citrus chachiensis Hort. (Citrus iyo), Fructus Citri grandis (Citrus junos), breast Fructus Citri tangerinae (Citrus kinokuni), Fructus Citri Limoniae (Citrus limon), Fructus Citri (Citrusmedica), Xia Cheng (Citrus natsudaidai), grapefruit (Citrus paradise), early red (Citrus reticulate), Fructus Citri sinensis (Citrus sinensis), bitter orange (Citrussphaerocarpa), acid Fructus Citri tangerinae (Citrus sudachi), three essentials-essence Citrus chachiensis Hort. (Citrus sulcata), upright flower Fructus Citri tangerinae (Citrus tachibana), Wenzhou Citrus (Citrus unshiu), the Carissa carandas of Apocynaceae Carissa (Carissa carandas), (Carissagrandiflora) spreads in the Cali, and, as plant of the raw material of quintessence oil etc.

As the plant that contains Carvacryl acetate (CAS:6380-28-5), enumerate the Cuminum cyminum L (Cuminum cyminum) of Umbelliferae Fructus Cumini Cymini apium, the Fructus Citri Limoniae of Verenaceae Lippia crosses river rattan (Lippia citriodora), Zimbabwe (Lippia javanica), cross river rattan (Lippia nodiflora), Lippia maltuflora, Folium Perillae Coe blows the Thymus decassatus that Herba thymi vulgaris belongs to, Thymus sipyleus, Thymus thracicus, Herba thymi vulgaris (Thymus vulgaris), Herba thymi vulgaris (Thymus zygis), the northern Rhizoma et radix valerianae of Valerianaceae valeriana (Valeriana fauriei), tender stem Rhizoma et radix valerianae (Valerianaflaccidissima), Rhizoma et radix valerianae (Valeriana officinalis) etc.

As the plant that contains Kessazulen (CAS:489-84-9), enumerate the Absinthium (Artemisia absinthium) of Compositae artemisia, Herba Artemisiae Annuae (Artemisia apiacea), Herba Artemisiae Scopariae (Artemisia capillaris), tarragon (Artemisia dracunculus), Artemisia maritime, Folium Artemisiae Argyi (Artemisia princeps), the Australia cypress (Callitris calcarata) that the Cupressaceae cypress belongs to, Bai Song (Callitris cupressiformis), cloudy fragrant (Cinnamomum burmanni), Camphor tree (Cinnamomum camphora), Cortex Cinnamomi (Cinnamomum cassia), Cinnamomum japonica (Cinnamomum japonica), yellow Camphor tree (Cinnamomum parthenoxylon), Cortex Cinnamomi (Cinnamomumseiboldii), Cinnamomum zeylanicum Bl. (Cinnamomum verum), the Eugenia banderensis of Myrtaceae Genus Syringa, the fragrant Flos Pelargonii (Geranium macrorrhizum) of the coconut palm of Mang cattle Seedling section Geranium, nepal cranesbill (Geraniumnepalense), the Laurencia obtuse that is subordinate to the Rhodomelaceae Laurencia, the Flos Matricariae chamomillae of Compositae Anthemis (Matricaria chamomilla), the fragrant peafowl flower (Pavonia odorata) of Malvaceae Hibiscus etc.

As the plant that contains dihydroconiferyl alcohol (CAS:2305-13-7), enumerate Pinus densiflora (Pinus densiflora), Pinus koraiensis (Pinus koraiensis), siberian dwarf pine (Pinus pumila), the Lapland pine (Pinus sylvestris) of Pinaceae Pinus, golden larch (Pseudolarix amabilis) of Pinaceae Pseudolarix etc.

As the plant that contains stevioside (CAS:57817-89-7), enumerate this Flos Chrysanthemi of Compositae (Stevia rebaudiana) etc. than subgenus.

As the plant that contains DL-australene (CAS:2437-95-8), enumerate Myrtaceae Eucalyptus Semen Armeniacae Amarum eucalyptus globulus (Eucalyptus amygdalina), grey eucalyptus globulus (Eucalyptuscinera), Eucalyptus globulus Labill (Eucalyptus globulus), white gum (Eucalyptusleucoxylon), Eucalyptus regnans and, as plant of the raw material of quintessence oil etc.

Verbenalin (CAS:548-37-8) is the chemical compound that another name is called Cornin.As the plant that contains verbenalin, enumerate the head Dendronenthamia japonica var.chinensis (Cornus capitata) of Cornaceae Macrocarpium, Canada's tatarian dogwood (Cornus canadensis), Fructus Corni (Cornus controversa), the Fructus Corni (Cornus florida) of blooming, Chinese kouse dogwood (Cornus kousa), strain wood (Cornus macrophylla), the West Fructus Corni (Cornus mas), Fructus Corni (Cornus officinalis), the beard-tongue (Pentstemon frutescens) that the Scrophulariaceae beard-tongue belongs to, beard-tongue (Pentstemongloxinioides), Pentstemon nitidus, the beauty cherry of Verenaceae Verbena (Verbena hybrida), Herba Verbenae (Verbena officinalis), Verbenaperuviana, rough leaf beauty cherry (Verbena rigida), Verbena stricta, spire beauty cherry (Verbena tenera) etc.

As the plant that contains 1-heptacosanol (CAS:2004-39-9), enumerate the Folium Agaves variegatae (Agave americana) that goat encyclopaedia goat hundred belongs to, Agave cantula Roxb (Agave cantala), imperial concubine's silk (Agave filifera), Herba Tephrosiae purpureae Folium Agaves Sisalanae (Agavefourcroydes), Thunder God (Agave potatorum), the snow (Agave victoriae-reginae) of fine jade fiber crops (Agave sisalana) Bamboo grass, the coloquintida of melon section Citrullus (Citrullus colocynthis), Citrullus vulgaris (Citrullus lanatus), the Da Ye of pulse family beggar-ticks takes body (Desmodium laxiflorum), leatherleaf Herba podocarpii oxyphylli (Desmodium oldhamii), Desmodium paniculatum, Desmodiumpadocarpum, the Fructus Chaenomelis banyan of Moraceae Ficus (Ficus auriculata), Vegetarian gelatin (Ficus awkeotsang), Indian ficus (Ficus bengalensis), banyan (Ficus benjamina), Fructus Fici (Ficus carica), triangle banyan (Ficusdiversifolia), rubberplant (Ficus elastoca), short and small Fructus Fici Beecheyanae (Ficuserecta), qin leaf rubber tree (Ficus lyrata), Ficus microcarpa Linn. f (Ficus microcarpa), Ficus oxyphylla, Caulis fici pumilae (Fructus Fici Pumilae) (Ficus pumila), bodhi tree (Ficus religiosa), red fruit fig-tree (Ficus superba), Xi Kemo Fructus Fici (Ficus sycomorus); the siegesbeckia glabrescens Makino (Siegesbeckia glabrescens) that chrysanthemum section Herba Siegesbeckiae belongs to; Herba Siegesbeckiae (Siegesbeckia orientalis) etc.

As the microorganism that contains 4 hydroxy coumarin (CAS:1076-38-6), enumerate Penicillium penicillium camembertii (Penicillium camembertii), penicillium (Penicillium jensenii), select penicillium sp (Penicillium notatum), cheese fungus (Penicillium roquefortii) etc.As the plant that contains 4 hydroxy coumarin, enumerate the Agelaea nitida that the Connaraceae Agelaca trinervis (Llanos) Merr. belongs to, Agelea obligua, Ageleatrifolia, the Byrsocarpus coccineus that Connaraceae Byrsocarpus belongs to, the Cnestis corniculatus that the Connaraceae stinging hair really belongs to, Cnestis ferruginea, the Ferula arrigonii of celery section Foeniculum, grass of Resina Ferulae (Ferula assafoetida), Fructus Foeniculi (Ferula communis), Ferula involucrata, Ferula kuhistanica, many stone Resina Ferulae (Ferula lapidosa), Ferula latipinna, Ferula leucographa, woods gram Resina Ferulae (Ferula linkii), Ferula sinaica, accurate Ge Er Resina Ferulae (Ferulasoongarcia), Ferula tenuiesta, Ferula ugamica, the Syria Folium Symplocoris Caudatae (Ruta chalepensis) that the Rutaceae Folium Symplocoris Caudatae belongs to, Folium Symplocoris Caudatae (Ruta graveolens) etc.

Cholic acid (CAS:81-25-4) is contained in most mammiferous bile.

As the microorganism that contains cholesteryl oleate (CAS:303-43-5), enumerate Anderson prickle Ticks (Dermacentor andersoni) etc.

As the plant that contains fraxin (CAS:524-30-1), enumerate the Acer aidzuense that the Aceraceae maple belongs to, big red autumnal leaves maple (Acer amoenum), sharp mouth maple (Acer argutum), Taiwan Acer buergerianum Miq (Acerbuergerianum), Taiwan Acer buergerianum Miq (Acer campestre), manger's leaf maple (Acer carpinifolium), tie up strange Folium Crataegi maple (Acer crataegifolium), ghost maple (Acer diabolicum), two lean on maple (Acer distylum), Acer ginnala Maxim. (Acer ginnala), quince (Acerjaponica), maple wood (Acer mono) Ash leaf maple (Acer negundo), black maple (Acer nigrum), comospore maple (Acer nikoense), Japan maple (Acernipponicum), Acer palmatum (Acer palmatum), Norway maple (Acerplatanoides), rock maple (Acer pseudoplatanus), close colored maple (Acerpycnanthum), brown arteries and veins maple (Acer rufinerve), Acer saccharum Marsh. (Acer saccharum), lobule is executed maple (Acer sieboldianum), regular script in small characters maple (Acer tschonoskii), flower pattern maple (Acer ukurunduense), the Aesculus chinensis Bunge of Hippocastanaceae Aesculus (Aesculus chinensis), Aesculus hippocastanum L. (Aesculus hippocastanum), Aesculus chinensis Bunge (Aesculus turbinate), U.S.'s white wax of Oleaceae Fraxinus (Fraxinus americana), white beeswax (Fraxinus chinensis), gold leaf Ou Zhou Ash tree (Fraxinus excelsior), Cold boiled chicken oil (Fraxinus griffithii), Da Ye wax tree (Fraxinus hynchophylla), Japan's Ash (Fraxinus japonica), Fraxinus lanuginose, Cortex Fraxini mandshuricae (Fraxinus mandshurica), grey cured wood (Fraxinus ornus), Fraxinus oxyphylla, Japan's Honshu Ash (Fraxinus spaethiana), Fraxinus sieboldiana, the narrow leaf Pericarpium Citri tangerinae (Vaccinium angustifolium) of Ericaceae Vaccinium, wild lagophthalmos Pericarpium Citri tangerinae (Vaccinium ashei), Vaccinium australe, Fructus Vaccinii Bracteati (Vacciniumbracteatum), Fructus Pyracanthae Pericarpium Citri tangerinae (Vaccinium hirtum), bar Pericarpium Citri tangerinae (Vaccinium japonicum), big fruit Pericarpium Citri tangerinae (Vaccinium macrocarpon), Vaccinium myrtillus L. (Vaccinium myrtillus), gland tooth Pericarpium Citri tangerinae (Vaccinium oldhami), crowberry (Vaccinium oxycoccus), cherry blueberry (Vacciniumpraestans), Vaccinium smallii, bog bilberry (Vacciniumuliginosumu), Pericarpium Citri tangerinae (Vaccinium vitis-idaea), the weigela florida (Weigela florida) that Caprifoliaceae brocade band belongs to etc.

As the plant that contains bergaptol (CAS:486-60-2), enumerate the LAIMENG (Citrus aurantifolia) of Citrus section Citrus, Citrus aurantium Linn. (Citrus aurantium), Fructus Citri Sarcodactylis (Citrus bergamia), pomelo (Citrus grandis), eight the first day of the lunar month (Citrushassaku), she gives Citrus chachiensis Hort. (Citrus iyo), Fructus Citri grandis (Citrus junos), breast Fructus Citri tangerinae (Citruskinokuni), Fructus Citri Limoniae (Citrus limon), Fructus Citri (Citrus medica), Xia Cheng (Citrus natsudaidai), grapefruit (Citrus paradise), early red (Citrusreticulate), Fructus Citri sinensis (Citrus sinensis), bitter orange (Citrus sphaerocarpa), acid Fructus Citri tangerinae (Citrus sudachi), three essentials-essence Citrus chachiensis Hort. (Citrus sulcata), upright flower Fructus Citri tangerinae (Citrustachibana), Wenzhou Citrus (Citrus unshiu), the thick leaf disc floret wood (Dorstenia contrajerva) of the smelly Morus of Moraceae, Dorstenia elliptica, the Fructus Chaenomelis banyan of Moraceae Ficus (Ficus auriculata), Vegetarian gelatin (Ficus awkeotsang), Indian ficus (Ficus bengalensis), banyan (Ficus benjamina), Fructus Fici (Ficus carica), triangle banyan (Ficus diversifolia), rubberplant (Ficuselastoca), short and small Fructus Fici Beecheyanae (Ficus erecta), qin leaf rubber tree (Ficus lyrata), Ficus microcarpa Linn. f (Ficus microcarpa), Ficus oxyphylla, Caulis fici pumilae (Fructus Fici Pumilae) (Ficus pumila), bodhi tree (Ficus religiosa), red fruit fig-tree (Ficus superba), Xi Kemo Fructus Fici (Ficus sycomorus), the Radix et Rhizoma Notopterygii of Umbelliferae Notopterygium (notopterygiumfranchetii), plant Rhizoma Et Radix Notopterygii (notopterygiuminci sium), Rhizoma Et Radix Notopterygii (notopterygium rhizomes) etc.

Agent for promoting vitamin C transporter production of the present invention can use somewhat-white magnolia Drymotaenium miyoshianum (Mak.) Mak., Semen Armeniacae Amarum, sweet tea, grapefruit, Radix Ophiopogonis, Caulis Hederae Sinensis, Saponaria officinalis, Herba Houttuyniae, Flos Matricariae chamomillae, gambir, Radix Hamamelidis Mollis, Mulberry, Testa oryzae, Herba Equiseti Arvinsis etc.These can use a kind separately, in addition, and use more than 2 kinds also capable of being combined.In addition, these plants can be used any of its ground product, its extract or extraction hydrolysate.

Somewhat-white magnolia Drymotaenium miyoshianum (Mak.) Mak. (Magnolia obovata) is meant following plant: the deciduous tree of Magnoliaceae Magnolia, be distributed in from Hokkaido to nine divisions of China in remote antiquity and China, and grow in the mountain region, planted into flower garden tree, park tree building material etc.Refer to that also formal name used at school is Magnoliahypoleuca.Contain magnolol, magnolol of alkaloidal magnocurarine, magnoflorine, lignan class etc. in the bark of medicinal part, be used for anesis such as stomachache, diarrhoea, vomiting.

Almond (Prunus dulcis) is meant following plant: the deciduous tree of Rosaceae Prunus, and be grown in the few region of rain in summer and be suitable for warm water and soil, now, littoral various countries, Mediterranean and California, USA are main product ground.Refer to that also formal name used at school is Prunusamygdalus, Prunus communis, Amygdalus dulcis, Amygdaluscommunis, Amygdalus sativus.The oil that obtains of squeezing seed be used for emulsifying agent, massage oil, spice, liqueur manufacturing, make cake etc.Semen pruni armeniacae is not owing to contain amygdalin as cyanogenic glycoside, thereby can eat raw, is used as nut with seasonings such as butter, salt and uses.

Sweet tea (Hydrangea macrophylla) is meant following plant: the machaka of Saxifragaceae hydrangea, and wooden or medicinal and cultivate as the front yard.Pleasantly sweet in the plant, the Saccharum Sinensis Roxb. purposes is used as an alternative.Sweet ingredient is known as leaf stripe, different leaf stripe, and dried leaves is used as sweet tea-drinking and uses.

Grapefruit (Citrus paradise) is meant: the plant of Citrus section Citrus is the aiphyllium in warm place cultivation.Sarcocarp softness, succulence, contain 1～2% citric acid, tart flavour strong slightly, because of limonin has bitterness, size and summer orange are quite.Yellowish pink because of kind different, for yellow to red.Redness is produced by lycopene.Be used to eat raw or fruit juice etc., but major part is processed to freeze concentration fruit juice.

Radix Ophiopogonis, (Ophiopogon jponicus) was meant following plant: the pteridophyte of Ophioglossaceae (Ophiogrossaceae) Ophioglossum (Ophiogrossum) is distributed in from Hokkaido to nine divisions of China in remote antiquity and Chinese, the Korea peninsula.Another name be also referred to as dragon must, the portion of expanding of root is called as Radix Ophiopogonis, that Radix Ophiopogonis is used to nourish is strong, antitussive, eliminate the phlegm etc.

Caulis Hederae Sinensis (Hedera helix) is meant following plant: be the Araliaceae Hedera, be distributed widely in the trailing plant in Europe, north African.Another name is also referred to as foreign Caulis Hederae Sinensis (English ivy) etc.Leaf is used to treat calculosis, yellow disease etc.

Saponaria officinalis (Saponiria officinalis) is meant: be the plant of Caryophyllaceae Soapwort, the former herba perennis that originates from Europe.Another name is also referred to as common Soapwort.In addition, formal name used at school also is expressed as Silence saponalia.As medicinal, use the combination of leaf and rhizome to be used in dermatosiss such as eczema.Replace soap to use since ancient times in Europe.

Herba Houttuyniae (Houttuynia cordata) is meant: the plant that Herba Houttuyniae section Herba Houttuyniae belongs to is distributed in the herba perennis of Honshu, four countries, nine divisions of China in remote antiquity, Okinawa and Taiwan, China, Himalaya, Java etc.The dry thing of the overground part of Herba Houttuyniae uses as analgesic, detoxifcation, diuresis, eczema, antibiotic medicine.Known, distinctive foul smell comes from aldehydes such as lauryl aldehyde or capraldehyde.Be used in the Asia medicinal, but the America and Europe also as view and admire with and cultivate.

Flos Matricariae chamomillae (Matricaria chamomilla) is meant: the former 1 year living plant of grass that originates from the Compositae Anthemis in Europe.Another name is also referred to as Germany Flos Matricariae chamomillae, German Flos Matricariae chamomillae, Chamomile.With medicinal purpose and with the warm area cultivation headed by the European various places, the head inflorescence of medicinal part is used to diaphoresis, anthelmintic purpose, the quintessence oil that extracts from the Flos Matricariae chamomillae odorant, perfume, the shampoo that are used to liqueur, medicated cigarette in addition.

Gambir (uncaria gambir) is the plant of Rubiaceae wild gambier, is distributed in the Malay Peninsula, Sumatera, Brunei etc., is the evergreen low wood of climing property in the cultivation of various places, Asia, the southeast.Use leaf and sprig as medicinal part, with mitigation have blood in stool, hematuria, dysentery be that purpose is used.In addition, also use as mouthful raw material of interior freshener.As same purpose, also use the heartwood of catechu (Acacia catechu).

U.S. Radix Hamamelidis Mollis (Hamamelis virginiana) is meant: the former plant that originates from Eastern North America to the Hamamelidaceae Hamamelis in south.Leaf has as medicinal tea by the history that American Indian uses, and is used for cathartic, hemorrhoid.Contain the Radix Hamamelidis Mollis tannin in known leaf and the bark.

Mulberry (Morus alba) is meant the plant of the Moraceae Morus that East Asia originates in, and is also claimed another name to be Tang Sang, Morus alba, Bai Shisang.In the traditional Chinese medical science sprout being called gentle branch, leaf is called the skin that gentle leaf, fruit be called gentle mulberry, root and is called gentle Rhizoma Euonymus.Gentle branch is used for rheumatism and neuralgia etc., and it is analgesic that gentle leaf is used for, and that gentle mulberry is used to nourish is strong, anemia, support hair, and gentle Rhizoma Euonymus is used for antiinflammatory diuresis, antitussive, hypertension.

Testa oryzae (Oryza sativa) is meant: the plant of this genus of grass family standing grain, and as the bran of the rice of the staple food of Japan.

Herba Equiseti Arvinsis (Equisetum arvense) is the pteridophyte of Equisetaceae Equisetum, and the nutrition stem is called as Herba Equiseti Arvinsis, and the sporozoite stem is called as small scouring rush.Herba Equiseti Arvinsis and small scouring rush bear from same subterraneous stem, and early spring, at first small scouring rush stretched out, and Herba Equiseti Arvinsis is launched then.Herba Equiseti Arvinsis in addition, is used for dermatosis, dermatitis rhus etc. as medicinal and bedding and clothing are used for diuresis, hemostasis, antitussive, analgesic as external.

The plant and the plant of the present invention that comprise the chemical compound of being found by above-mentioned the present invention of enumerating can be used leaf, stem, bud, flower, xylem, veneer portion overground parts such as (barks), and all sites such as underground part, seed, resin such as root, tuber.In addition, the microorganism that comprises the chemical compound of being found by above-mentioned the present invention of enumerating can be used for example liquid culture, solid culture, in the situation of mushroom, can be used for fungus strain body, sporophore etc. any.

The above-mentioned plant, mineral, microorganism, the animal of enumerating that comprise the chemical compound of being found by the present invention can be used as hydrolysate, the extract of substance, substance, the hydrolysate of extract uses.In addition, even if these chemical compounds also can use for the material of chemosynthesis.

As the extracting method that is used for obtaining chemical compound of the present invention from plant, mineral, microorganism, animal, for example under the situation of grapefruit, be listed below method: extract squeezing the juice of obtaining of the exsiccant dry thing of grapefruit, its ground product, squeezing grapefruit with appropriate solvent etc., according to circumstances implement hydrolysis process, remove insoluble part by filtering to wait, remove from this liquid desolvate after, use common purification scheme such as column chromatography to obtain the method for target compound.

As the solvent that is used to extract, enumerate alcohols such as methanol, ethanol, propanol, 1,3 butylene glycol, organic solvents such as ether, acetone, ethyl acetate, hexane, cyclohexane extraction, dichloromethane, chloroform, water etc.These solvents can use separately, also can mix more than 2 kinds and use.Extraction conditions is not particularly limited, and for example temperature is 5～95 ℃, is preferably 15～85 ℃, also can suitably extract at normal temperatures.Pressure can be any of normal pressure, pressurization, decompression.Extraction time is different because of selected solvent, is several minutes～a few hours, can be by the raising extraction efficiencies such as prolongation of extract repeatedly, vibrate extraction, extraction time.In addition, when being hydrolyzed, enumerate decomposition that alkali such as decomposition that acid such as decomposition that microorganisms such as decomposition, lactobacillus and yeast that the such enzyme of esterase carries out carry out, hydrochloric acid, sulphuric acid, acetic acid, phosphoric acid, citric acid, lactic acid, tartaric acid, fumaric acid, malic acid carry out, sodium hydroxide, potassium hydroxide carry out etc.The condition of hydrolysis is not particularly limited, and when using enzyme or microorganism, according to optimum temperature, the optimum pH of employed enzyme or microorganism, 10～55 ℃, pH3～8, carries out hydrolysis about 5～24 hours usually.When using acid or alkali to be hydrolyzed, ℃ carrying out hydrolysis about 0.5～24 hour under the concentration of 2～15 weight % in room temperature～60 usually.

The hydrolysate of these extracts or extract can obtain concentrated dry thing by known method such as lyophilization, distilling under reduced pressure, reduced vacuum drying, spray dryinges after adjusting pH as required.The method of purification processes is not particularly limited, and for example combinations such as the purification undertaken by positive and reversed-phase column chromatography, recrystallization, redeposition, decolouring processing, deodorize processing can be purified.

By extract and the cited chemical compound of the present invention of the cited plant of the extract that contains grapefruit, the present invention of said method extraction, in the analysis of using cell, can confirm significantly to have promoted the expression of vitamin C transporter.Hence one can see that, agent for promoting vitamin C transporter production of the present invention as vitamin C absorb with compositions, to whiten with compositions and collagen generation be useful with compositions.

" agent for promoting vitamin C transporter production " among the present invention improved sodium dependency vitamin C transporter 1 type (SVCT1) or any one of 2 types (SVCT2) or both expression, in other words, improved as any one or both of SLC23A1 or SLC23A2 gene expressed the expression of product.

" vitamin C absorption composition for promoting " among the present invention is meant: the material of the above combination of at least one of agent for promoting vitamin C transporter production and vitamin C or derivatives thereof, vitamin C, the vitamin C concentration high compositions of or derivatives thereof in the target internal organs absorbed by per os or coating.

As " vitamin C derivatives " among the present invention, except 2-O-α-D-glycopyranosyl-ascorbic acid, also can enumerate ascorbic acid monoalkyl esters such as L-ascorbic acid monostearate, L-ascorbic acid monopalmitate, L-ascorbic acid monoleate; L-ascorbic acid phosplate, L-ascorbic acid-ascorbic acid monoester derivates such as 2-sulphuric acid; Ascorbic acid dialkyl esters such as L-Ascorbic acid distearate, L-Vitamin C dipalmitate, L-ascorbic acid dioleate; Ascorbic acid trialkyl esters such as ascorbic acid diester derivs such as L-ascorbic acid bisphosphate, L-ascorbic acid tristearate, L-ascorbic acid tripalmitate, L-ascorbic acid trioleate; Ascorbic acid three ester derivants such as L-ascorbic acid triguaiacyl phosphate etc., but be not limited to these.As salt, can enumerate the salt of ascorbic acid and various alkali.For example alkali metal salt (for example sodium salt etc.), alkali earth metal salt (for example calcium salt, magnesium salt etc.) etc., but be not limited to these.

" whiten and use compositions " among the present invention is meant the compositions that original pigmented spots, freckle is shown the desalination effect.Pigmented spots, freckle are that vitamin C is the obstruction material that generates the tryrosinase that much relations are arranged with melanocyte owing to melanocyte generates result excessive and that deposition takes place, and also known its promoted the effect that becomes reduced form light color melanocyte from oxidized form heavy colour melanocyte.Reported the pigmentation (non-patent literature 7: Pro skin 1967.Vol 21 (7) .725-729) that improves existing pigmented spots, freckle etc. clinically.Of the present invention whitening is meant the compositions that strengthens ascorbic whitening function by the vitamin C concentration in the raising Skin Cell with compositions.As long as of the present invention whitening comprises vitamin C of the present invention with compositions and absorbs composition for promoting, and then, only otherwise hinder vitamin C absorption facilitation and then also can add additive adjustment separately.

" collagen produce use compositions " among the present invention is meant: by improving the compositions that vitamin C concentration strengthens the collagen generation facilitation that vitamin C has.For example, when collagen generation cell is Skin Cell, refers to and improve the moist compositions of skin, when collagen generation cell is osteoblast, refer to the compositions that strengthens bone strength.Collagen of the present invention produces and comprises vitamin C of the present invention with compositions and absorb composition for promoting and get final product, and then, only otherwise hinder collagen generation facilitation and then can also add additive adjustment separately.

" glucosidase agent " among the present invention is meant: himself have the material of glucosidase activity or make the activatory material of glucosidase activity.As the material that himself has glucosidase activity, enumerate for example yeast, bacillus bifidus, lactobacillus, and then, can also use the glucosidase of the microorganism that is derived from Streptococcus, Eurotium, streptomyces, Rhizopus, Fusobacterium, streptomyces etc., these are restriction not.In addition, as making the activatory material of glucosidase activity, can enumerate the Echinacea Species (patent documentations 1: TOHKEMY 2005-029546 communique), but be not limited to this such as (Echinacea purpurea, Echinaceaangustifolia, Echinacea pallida, Echinacea simulata, Echinaceaparadoxa, Echinacea atrorubens) of Compositae Echinacea.Glucosidase agent of the present invention can directly be pulverized use with above-mentioned raw material, in addition, can use above-mentioned raw-material extract, extract hydrolysate.

Of the present invention dose or compositions can be used as uses such as food, pharmaceuticals, cosmetics.As food, except common food, also can be used as uses such as dietary supplement, functional food, health food, specific food for health care, also can be coupled in the such beverage of fruit juice for example.

In the pharmaceuticals,, with effective ingredient oral administration, non-oral uptake the time, can mix, with the form administration of habitual pharmaceutical preparation with the solid that is suitable for medications such as drop rectum with drug, injection or the pharmaceutical non-toxic carrier of liquid about administration.As form, enumerate liquor, freeze-dried preparation of for example solid agents such as powder, powder, granule, tablet, capsule, solution, suspending agent, Emulsion etc. etc., these preparations can be modulated by conventional scheme.As above-mentioned pharmaceuticals non-toxic carrier, enumerate for example glucose, lactose, sucrose, starch, mannitol, dextrin, fatty glyceride, Polyethylene Glycol, hydroxyalkyl vinyl starch, ethylene glycol, polyoxyethylene sorbitan aliphatic ester, aminoacid, gelatin, albumin, water, normal saline solution etc.Also can suitably add additives such as stabilization agent, wetting agent, emulsifying agent, bonding agent, tonicity agent as required.

In of the present invention dose or the compositions, effective dosage of chosen chemical compound waits according to object person's age, body weight, symptom, administration path, administration schedule, preparation form and suitably selects decision, for example under the situation that per os is used, the dosage of stevioside is preferably per 1 day 1～5000mg, is preferably 10～1000mg especially.In addition, vitamin C is preferably per 1 day 10～2000mg, is preferably 100～1000mg especially.Can be with it by the administration for several times of 1 bu.

In of the present invention dose or the compositions, effective dosage of the ground product of chosen plant, animal, mineral, microorganism, dry thing, extract, extraction hydrolysate according to age of object person, body should, symptom, administration path, administration schedule, preparation form, raw-material activity intensity wait and suitably select decision, for example in the per os time spent, the dosage of Herba Houttuyniae extract is preferably per 1 day 1～50000mg, is preferably 10～1000mg especially.In addition, vitamin C is preferably per 1 day 10～2000mg, is preferably 100～1000mg especially.Can be with it by the administration for several times of 1 bu.

In addition, of the present invention dose or compositions can be made into so-called cosmetics in the medicine thing method, medicine part outer article, the medium contained product of pharmaceuticals as external preparation for skin.Be used for the such skin-protection product of emulsion, cream, lotion, ointment etc.Though be not particularly limited, this dosage form can obtain dosage form widely such as water solution system, solubilising system, emulsion type, powder system, fluid system, gel system, ointment system, Sol SYSTEMS, water-oily 2 series of strata, water-oil-powder 3 series of strata.

In of the present invention dose or the compositions, the content in the external preparation for skin of chosen chemical compound is suitably selected according to the difference of symptom, when for example being marrubilin, about 0.001～10 weight % for gross weight, be preferably 0.01～2 weight %.In addition, when vitamin C derivatives uses 2-O-α-D-glycopyranosyl-ascorbic acid, about 0.001～50 weight %, be preferably 0.1～10 weight % for gross weight.It is also harmless certainly by 1～4 time/day coating.

In of the present invention dose or the compositions, content is suitably selected according to the difference of symptom in the external preparation for skin of chosen plant, when for example being grapefruit extract, about 0.001～50 weight % for gross weight, be preferably 0.01～10 weight %.In addition, when vitamin C derivatives uses 2-O-α-D-glycopyranosyl-ascorbic acid, about 0.001～50 weight %, be preferably 0.1～10 weight % for gross weight.It is also harmless certainly by 1～4 time/day coating.

Below enumerate embodiment and specify, but be not limited to this.

Embodiment 1

(extraction of plant)

At somewhat-white magnolia Drymotaenium miyoshianum (Mak.) Mak. (Magnolia obovata), Semen Armeniacae Amarum (Prunus dulcis), sweet tea (Hydrangea macrophylla), grapefruit (Citrus paradise), Radix Ophiopogonis (Ophiopogon jponicus), Caulis Hederae Sinensis (Hedera helix), Saponaria officinalis (Saponiria officinalis), Herba Houttuyniae (Houttuynia cordata), Flos Matricariae chamomillae (Matricaria chamomilla), gambir (uncalia gambir), Radix Hamamelidis Mollis (Hamamelis virginiana), Mulberry (Morus alba), Testa oryzae (Oryza sativa), add 90% ethanol liquid 12L (liter) among the dry thing 1000g of Herba Equiseti Arvinsis (Equisetum arvense), 40 ℃ were stirred 5 hours down, reclaim filtrate with filter paper.Repeating 2 times should operate, and with vaporizer filtrate decompression was concentrated, and removed alcohol solvent.Its result obtains the dry weight of somewhat-white magnolia extract of magnolia 120.6g, Semen Armeniacae Amarum extract 150.4g, sweet tea extract 170.9g, grapefruit extract 127.3g, Radix Ophiopogonis extract 115.3g, Caulis Hederae Sinensis extract 204.8g, Saponaria officinalis extract 143.7g, Herba Houttuyniae extract 133.9g, Flos Matricariae chamomillae extract 193.2g, gambir extract 168.2g, Radix Hamamelidis Mollis extract 112.0g, Mulberry sleeve mountain thing 103.8g, Testa oryzae extract 184.7g, Herba Equiseti Arvinsis extract 102.7g.

Embodiment 2

(generation of the vitamin C transporter in the hepatocyte (SVCT) promotes active mensuration)

The culture dish (Off ア Le コ Application society system) that the HepG2 cell (Dainippon Pharmaceutical Co., Ltd's system) that is derived from human liver cancer is planted diameter 3.5cm makes and to become 5 * 10 ³Cells in containing the DMEM culture fluid (ギ Block コ society system) of 10% inactivated fetal bovine serum (FBS), cultivated 3 days in the presence of 37 ℃, 5% carbon dioxide.Postposition changed new culture fluid in 3 days, and as being tested the cell that material adds usefulness.Buy in from Off Na コ シ society as the marrubilin of being tested material, cholesteryl benzoate, ugaferine, lapiferine, geranyl acetate, nerolidol, dihydro jasmone, Carvacryl acetate, Kessazulen, dihydroconiferyl alcohol, stevioside, DL-australene, verbenalin, 1-heptacosanol, 4 hydroxy coumarin, cholic acid, cholesteryl oleate, fraxin, bergaptol.Tested substance dissolves in dimethyl sulfoxine (DMSO), added in the cell of being tested material interpolation usefulness to making that ultimate density is 100 μ M, after 6 hours, used TRIZOL reagent (イ Application PVC ト ロ ジ ェ Application society system), reclaimed full RNA by conventional method.The full RNA that reclaims uses First-StrandcDNAstrandcDNA Synthesis kit (ア マ シ ヤ system バ イ オ サ イ エ Application ス society system) to be transformed into cDNA, supplies in PCR in real time.PCR in real time is come modulation template according to QuantiTectSYBRGreen PCR Kit (キ ア ゲ Application society system), uses PCR in real time device (MJ リ サ one チ society system) to measure the activity of the expression that promotes SVCT1, SVCT2 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA.Known GAPDH gene is as house-keeping gene, all expressing consistently in the cells, therefore, is widely used as the crt gene of internal standard.In addition, quilt being tested material does not have interpolation (DMSO0.1%) and organizes in contrast.PCR is as follows with primer and PCR condition.

＜primer 〉

SVCT1 uses

5 '-TTC TGA TTGTGC TGC TGACC-3 ' (sequence table serial number 1)

5 '-ATG TCA CTGTTG GCA TGAGC-3 ' (sequence table serial number 2)

SVCT2 uses

5 '-GGA ACC TCTTTG TGC TTGGA-3 ' (sequence table serial number 3)

5 '-CCT GGG ATGGTG TTA TCCAG-3 ' (sequence table serial number 4)

GAPDH uses

5 '-CGA CCA CTTTGT CAA GCTCA-3 ' (sequence table serial number 5)

5 '-TTC CTC TTGTGC TCT TGCTG-3 ' (sequence table serial number 6)

The reaction condition of＜PCR 〉

Close and do SVCT1 and SVCT2

With (i) 95 ℃, 15 minutes → (ii) 94 ℃, 15 seconds → (iii) 55 ℃, 30 seconds → (iv) 72 ℃, 30 seconds be 1 circulation, and then repeat 35 times (ii)～(iv) after, preserve down at 4 ℃.

About GAPDH

With (i) 95 ℃, 15 fens kinds → (ii) 94 ℃, 15 seconds → (iii) 58 ℃, 30 seconds → (iv) 72 ℃, 30 seconds was 1 circulation, so repeat 35 times (ii)～(iv) after, preserve down at 4 ℃.

The correction of PCR primer is confirmed by the solubility curve of PCR product and the electrophoresis of RT-PCR product.Following calculating of the expression of target gene and evaluation:, and calculate the period (thresholdcycle:Ct value) that obtains from the intersection point of threshold value and PCR product amplification curve at the appropriate location setting threshold (threshold) of the exponential function amplification region of PCR product.Each following carrying out of evaluation of being tested the expression between material: the quilt of group is in contrast tested material not to be had the Ct value of mRNA of the SVCT1 of interpolations (DMSO0.1%) or SVCT2 the value of obtaining is made as 1 divided by the Ct value of GAPDH, the research of comparing of the expression when being tested the material interpolation with quilt.The results are shown in table 1 and table 2.

Its result as can be known, compare with matched group, the chemical compound that promotes SVCT1 and SVCT2 to express is marrubilin and cholesteryl benzoate, the chemical compound that only promotes SVCT1 to express is stevioside, DL-australene, verbenalin, 1-heptacosanol, 4 hydroxy coumarin, cholic acid, cholesteryl oleate, fraxin and bergaptol, and the chemical compound that only promotes SVCT2 to express is ugaferine, lapiferine, geranyl acetate, nerolidol, dihydro jasmone, Carvacryl acetate, Kessazulen and dihydroconiferyl alcohol.

In addition, use known dexamethasone with the effect that promotes that SVCT2 expresses to attempt carrying out active comparative evaluation with chemical compound of the present invention in osteoblast, dexamethasone be can't see the effect that promotes that SVCT2 expresses fully in hepatocyte.

Table 1

The generation of the vitamin C transporter in the hepatocyte (SVCT1) promotes active mensuration

Tested material	SVCT1/GAPDH (relative comparison ratio)
		Marrubilin	6.5
Cholesteryl benzoate	9.1
		Stevioside	5.0
The DL-australene	3.9
		Verbenalin	2.9
The 1-heptacosanol	2.9
		4 hydroxy coumarin	3.1
Cholic acid	3.3
		Cholesteryl oleate	3.9
Fraxin	5.7
		Bergaptol	3.8

Table 2

The generation of the vitamin C transporter in the hepatocyte (SVCT2) promotes active mensuration

Tested material

SVCT2/GAPDH (relative comparison ratio)

Marrubilin	4.3
		Cholesteryl benzoate	3.2
ugaferine	4.5
		lapiferine	3.7
Geranyl acetate	2.6
		Nerolidol	2.5
Dihydro jasmone	2.6
		Carvacryl acetate	2.5
Kessazulen	3.0
		Dihydroconiferyl alcohol	4.2

Embodiment 3

(generation of the vitamin C transporter in the melanocyte (SVCT) promotes active mensuration)

The culture dish (Off ア Le コ Application society system) that the NHEM cell (Network ラ ボ ウ society system) that is derived from human eumelanin cell is planted diameter 3.5cm makes and to become 5 * 10 ³Cells in containing the 254S culture fluid of special additive (Network ラ ボ ウ society system), cultivated 8 days in the presence of 37 ℃, 5% carbon dioxide.After 8 days, be replaced as new culture fluid and tested the cell that material adds usefulness as quilt.As being tested material, use the plant of extracting from embodiment 1, somewhat-white magnolia Drymotaenium miyoshianum (Mak.) Mak. (Magnolia obovata), Semen Armeniacae Amarum (Prunus dulcis), sweet tea (Hydrangea macrophylla), grapefruit (Citrusparadise), Radix Ophiopogonis (Ophiopogon jponicus), Caulis Hederae Sinensis (Hedera helix), Saponaria officinalis (Saponiria officinalis), Herba Houttuyniae (Houttuynia cordata), Flos Matricariae chamomillae (Matricaria chamomilla), gambir (uncalia gambir), Radix Hamamelidis Mollis (Hamamelis virginiana), Mulberry (Morus alba), Testa oryzae (Oryzasativa), Herba Equiseti Arvinsis (Equisetum arvense).Being tested material is dissolved into 50%1 again, making in the 3-butanediol water becomes 1% solid constituent, add to tested make in the cell that material adds usefulness that the ultimate density of this solution is 0.5%, 6 hour after, use TRIZOL reagent (イ Application PVC ト ロ ジ ェ Application society system), reclaim full RNA by conventional method.Used the expression evaluation of the PCR in real time of SVCT1, SVCT2 and GAPDH to implement by evaluation methodology similarly to Example 2.The results are shown in table 3.

Its result compares with matched group, and the plant extract that extracts from embodiment 1 has promoted the expression of SVCT2 as can be known.In addition, the known dexamethasone that has promotion SVCT2 expressional function in osteoblast be can't see the effect that promotes that SVCT2 expresses fully in melanocyte.In addition, the NHEM cell that is derived from human eumelanin cell that uses in the present embodiment is owing to can't see the expression of SVCT1, thereby can't be to the active implementation evaluation that promotes that SVCT1 expresses.

Table 3

The generation of the vitamin C transporter in the melanocyte (SVCT2) promotes active mensuration

Tested material (extract of embodiment 1)	SVCT2/GAPDH (relative comparison ratio)
		The somewhat-white magnolia extract of magnolia	1.4
The Semen Armeniacae Amarum extract	2.7
		Sweet tea extract	3.8
Grapefruit extract	1.9
		Radix Ophiopogonis extract	1.4
Caulis Hederae Sinensis extract	2.0
		The Saponaria officinalis extract	2.3
Herba Houttuyniae extract	1.5
		The Flos Matricariae chamomillae extract	2.4

The gambir extract	3.9
		The Radix Hamamelidis Mollis extract	2.3
The Mulberry extract	2.2
		The Testa oryzae extract	2.4
Herba Equiseti Arvinsis extract	2.5

Embodiment 4

(melanocyte generation inhibition test)

To be used in combination the plant that obtains among the present invention and vitamin C and to generate to the effect of whitening function and by following test determination melanocyte and suppress active in order to estimate.As the cell that generates melanocyte, use the B16-F10 melanoma cell that is derived from mice, plant and make in each holes of 48 orifice plates and become 2 * 10 ³Cells, add FBS so that ultimate density be 10% the minimum that contains glucosamine must culture fluid (MEM) in cultivation 5 days., culture fluid be exchanged for the MEM that contain 10%FBS, theophylline thereafter, and as being tested the cell that material adds usefulness.Tested material and used following material: 50%1, dissolve the somewhat-white magnolia Drymotaenium miyoshianum (Mak.) Mak., Semen Armeniacae Amarum, sweet tea, grapefruit, Radix Ophiopogonis, Caulis Hederae Sinensis, Saponaria officinalis, the Herba Houttuyniae that obtain among the embodiment 1 in the 3-butanediol water again, make that solid constituent is 1%.Add concentration according to the ultimate density of being tested material be 0.5% and simultaneously ascorbic ultimate density be that 100 μ g/ml add to and tested in the cell that material adds usefulness, cultivated 3 days, generate the sample of inhibition evaluation usefulness as melanocyte.The melanocyte amount is tried to achieve by following: by scraper handle with cell peel off, centrifugal after, by 1% sodium lauryl sulphate (SDS) solubilising, measure the absorbance of 475nm, 260nm and try to achieve.Melanocyte generates suppression ratio and calculates by following: with the absorbance of cultured cells in having added the culture fluid of being tested material of the absorbance of 475nm, 260nm as S ₄₇₅, S ₂₆₀, with the absorbance of cultured cells in not adding the culture fluid of being tested material of the absorbance of 475nm, 260nm as C ₄₇₅, C ₂₆₀, calculate by following formula 1.This results are shown in table 4.As shown in Table 4, add when being tested material separately, can't see melanocyte and generate the inhibition effect, but, then compare obvious inhibition melanocyte separately and generate with vitamin C by adding simultaneously with vitamin C.Hence one can see that, contains these and tested material and ascorbic compositions and have melanocyte and generate inhibit feature, Pear Power effect.

Formula 1

Melanocyte generates suppression ratio (%)=1-[(S ₄₇₅/ S ₂₆₀)/(C ₄₇₅/ C ₂₆₀)] * 100

Table 4

Melanocyte generates inhibition test

Embodiment 5

(melanocyte of three-dimensional skin model generates inhibition test)

In order to estimate the Echinacea Species extract that is used in combination the grapefruit extract that obtains among the present invention, uses effect, generate by following test determination melanocyte and suppress active whitening function as the 2-O-α-D-glycopyranosyl-ascorbic acid (AA2G) of vitamin C derivatives and as the glucoside enzyme activator.

As appraisement system, use the melanocytic three-dimensional skin model (MEL-300 test kit) that is derived from normal human subject that comprises of Network ラ ボ ウ society sale.Begin to cultivate according to the explanation of test kit, culture fluid and quilt are tested material and were exchanged for new material in per 2 days, carry out cultivating in 10 days.After 10 days, in phosphate buffer (PBS), behind the washing tissue, be recovered in the 1.5ml pipe, add distilled water 100 μ l, carry out ultrasonic disruption.And then, add 900 μ l Soluene-350 (with the pure medicine of light society system), left standstill 45 minutes at 100 ℃, make tissue and melanocyte solubilising, indissolvable component is removed by centrifugalize (10000rpm, 10 minutes).The mensuration of melanocyte is made standard curve by synthetic melanocyte (シ グ マ ア Le De リ Star チ society system) and is calculated by the absorbance of 500nm.

Tested material and used 50%1, dissolve the grapefruit that obtains among the embodiment 1 in the 3-butanediol water again so that solid constituent be 1% material (Gr), 2-O-α-D-glycopyranosyl-ascorbic acid (AA2G), with the Echinacea Species extracting solution (Ech) (containing 0.3% Echinacea Species extract (solid constituent)) of the dry thing of the leaf of 30% ethanol-water extraction Echinacea Species.The concentration of in three-dimensional skin model, adding according to the ultimate density of Gr be 0.5% or simultaneously the ultimate density of AA2G be 2% or simultaneously Ech 0.2% add, estimate respectively separately, the melanocyte when being used in combination generates and suppress active.It the results are shown in Fig. 1.

As shown in Figure 1 as can be known, grapefruit extracting solution (Gr), 2-O-α-D-glycopyranosyl-ascorbic acid (AA2G), Echinacea Species extracting solution (Ech) are not all compared difference that can't see significance with there being the matched group that adds separately the time, suppress active by being used in combination the melanocyte generation of seeing significance with 3.

Embodiment 6

(human skin is through the inhibition of the inflammation of ultraviolet radiation, the improvement test of pigmentation)

1. the tester uses the male's of health adult 10 (27～35 years old) back to experimentize.

2. test body and use, test body by the modulation of the composition shown in the following table 5 as the 2-O-α-D-glycopyranosyl-ascorbic acid (AA2G) of vitamin C derivatives with as the grapefruit extract (GFE) of agent for promoting vitamin C transporter production.

Below, use the body of testing of following composition to carry out evaluation test.

Table 5

	AA2G	GFE		1，3-BG	Purified water
						Embodiment
6	2.0％	0.003％	0.247％	Residue
					Comparative example 1	2.0％	0.0％	0.247％	Residue
Comparative example 2	0.0％	0.003％	0.247％	Residue
					Comparative example 3	0.0％	0.0％	0.247％	Residue

AA2G: the former system of woods 2-O-α-D-glycopyranosyl-ascorbic acid

CFE: the solid constituent among the ball Off ア Le コ ス system grapefruit fruit extract Off ア Le コ レ Star Network ス grapefruit B

1,3-BG:1,3-butanediol

3. test method

3.1 the inhibition Evaluation on effect of inflammation

To respectively test body at the back and set the dispensing area of 4 * 4cm, and be coated with and 8 weeks tested body.Per 1 the coating 3mL of coating weight, about 4 times of 1 week, 8 week about 32 times (28～35 times).After 8 weeks, measure the L at this position ^*a ^*b ^*The a of color specification system ^*Value, and then 1.2 times the solar ultraviolet of irradiation MED after 2 days, is measured a with the position ^*Value.The a of ultraviolet radiation after 2 days ^*Value deducts a before the ultraviolet radiation ^*Value is obtained Δ a ^*Value.Δ a ^*Be worth more for a short time, the inhibition effect of inflammation is high more.

3.2 pigmentation improve Evaluation on effect

To respectively test body at the back and set the dispensing area of 4 * 4cm, and measure and test the preceding L of body coating ^*a ^*b ^*The L of color specification system ^*Value.Then, 1.2 times the solar ultraviolet of irradiation MED after 2 days, is measured the L with the position ^*Value.From measuring the L after 2 days ^*Begin coating after the value and test body.Per 1 the coating 3mL of coating weight, about 4 times of 1 week, 8 week about 32 times (28～35 times).Ultraviolet radiation is measured the L with the position after 2,4,8 weeks ^*Value.Ultraviolet radiation is after 2 days and the L of ultraviolet radiation after 2,4,8 weeks ^*Value deducts the L that tests body coating preceding (before the ultraviolet radiation) ^*Value is obtained Δ L ^*Value.Δ L ^*The minus value of value is more little, and pigmentation improves more.

3.3 mensuration machine

Colour examining: MINOLTA system spectral photometric colour measuring meter CM-800D

Ultraviolet radiation: SOLAR LIGHT CO. system MODEL XPS 200

4. measurement result

4.1 the inhibition effect of inflammation is respectively tested the Δ a at body coating position ^*Value is shown in table 6, Fig. 2.

Table 6

Test body	Δa *Value
		Embodiment 6	0.82
Comparative example 1	1.33
		Comparative example 2	2.25
Comparative example 3	2.10

Only cooperate Δ a as the comparative example 2 of the GFE of agent for promoting vitamin C transporter production ^*Be worth Δ a with any comparative example that does not also cooperate 3 ^*Value is a value much at one.Therefore as can be known, only GFE then can't obtain the anti-inflammatory effect.On the other hand, be used in combination GFE and compare the anti-inflammatory effect that shows significance with comparative example 3 with the embodiment 6 of the AA2G of vitamin C derivatives.And, the Δ a of embodiment 6 ^*Value is reduced to the Δ a of the comparative example 1 that only cooperates AA2G ^*60% of value.Owing to can't see the anti-inflammatory effect of GFE self, thereby be used in combination GFE and compare with the comparative example 1 of independent cooperation AA2G with the embodiment 6 of AA2G, show excellent anti-inflammatory effect, this can be described as unpredictable significant effect.

4.2 pigmentation improve effect

Respectively test the Δ L at body coating position ^*Value is shown in table 7, Fig. 3.

Table 7

Test body	The UV pre-irradiation	The UV irradiation is after 2 days	After UV shone for 2 weeks	After UV shone for 4 weeks	After UV shone for 8 weeks
						Embodiment
6	0	-2.17	-0.95	-0.50	0.44
						Comparative example 1	0	-2.07	-1.57	-1.25	-0.09
Comparative example 2	0	-2.06	-2.23	-1.75	-0.15
						Comparative example 3	0	-2.02	-1.98	-1.92	-0.99

At ultraviolet radiation after 2 weeks, as the Δ L of the comparative example 2 of the GFE that only cooperates agent for promoting vitamin C transporter production ^*The Δ L of value and the comparative example 3 that does not cooperate any material ^*Value is compared minus value and is become big.Therefore as can be known, only GFE then can't see pigmentation and improves effect.On the other hand, the embodiment 6 that GFE and AA2G as vitamin C derivatives are used in combination compares with comparative example 3, shows that the pigmentation of significance improves effect.And, the Δ L of embodiment 6 ^*Value is reduced to the Δ L of the comparative example 1 that only cooperates AA2G ^*60% of the minus value of value is compared with comparative example 1, shows that the pigmentation of significance improves effect.Because pigmentation that can't see GFE self improves effect, thereby be used in combination GFE and compare with the comparative example 1 of independent cooperation AA2G with the embodiment 6 of AA2G and show that excellent pigmentation improves effect, this can be described as unpredictable significant effect.At ultraviolet radiation after 4 weeks, also obtain and ultraviolet radiation almost same effect after 2 weeks.After 8 weeks, it is also the most excellent that the pigmentation of embodiment 6 improves effect at ultraviolet radiation.Below enumerate Formulation Example and specify, but be not limited to this.

Embodiment 7

(generation of the vitamin C transporter of grapefruit extract (SVCT) promotes active mensuration)

Murine melanoma B16-F10 cell (Dainippon Pharmaceutical Co., Ltd's system) is planted make in the culture dish (Off ア Le コ Application society system) of diameter 3.5cm and become 5 * 10 ³Cells is in containing the DMEM culture fluid (ギ Block コ society system) of 10% inactivated fetal bovine serum (FBS), cultivate in the presence of 37 ℃, 5% carbon dioxide.Cultivate after 3 days, be replaced as new culture medium and interpolation as the grapefruit of being tested material (Citrusparadise) extract, make that the ultimate density in the culture medium is 0.6,1.8,6,18,60,180 μ g/ml, use TRIZOL reagent (イ Application PVC ト ロ ジ ェ Application society system) to reclaim full RNA after 6 hours by conventional method.As grapefruit extract, add the ball Off ア Le コ ス system grapefruit fruit extract Off ア Le コ レ Star Network ス grapefruit B that uses among the embodiment 6.This grapefruit extract is the following material that obtains: add 1 in the fresh fruit 5kg of grapefruit citrus paradise Macfadyen (Rutaceae), the 3-butanediol, dipping filters, in this filtrate 20kg, add purified water 20kg and mix, filter, thereby obtain.The concentration conversion of grapefruit extract is 0.6% (6mg/mL) in the grapefruit extracting solution during for solid component concentration.

The full RNA that reclaims supplies in real-time quantitative one step RT-PCR.According to QuantiTect SYBR Green PCR Kit (キ ア ゲ Application society system) modulation template, use PCR device (MJ リ サ one チ society system), measure the expression activity that promotes SVCT2 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA.PCR is as follows with primer and PCR condition.

＜primer 〉

SVCT2 uses

Mm_Slc23a2_1_SG QuantiTect Primer Assay (キ ア ゲ Application society system)

GAPDH uses

5 '-AAC TTT GGC ATT GTG GAA GG-3 ' (sequence table serial number 7)

5 '-ACA CAT TGG GGG TAG GAA CA-3 ' (sequence table serial number 8)

The reaction condition of＜PCR 〉

With (i) 50 ℃, 30 minutes → (ii) 95 ℃, 15 minutes → (iii) 94 ℃, 15 seconds → (iv) 55 ℃, 30 seconds → (v) 72 ℃, 30 seconds is 1 circulation, so repeat 40 times (ii)～(v), preserve down at 4 ℃.

The correction of PCR primer is confirmed by the solubility curve of PCR product and the electrophoresis of RT-PCR product.The following expression of the expression of target gene: at the appropriate location setting threshold of the exponential function amplification region of PCR product, and obtain period (Ct value) from the intersection point of threshold value and PCR product amplification curve, by calculating, with respect to numeric representation as the expression of the GAPDH of internal standard with following formula.

Formula 2

Rna expression amount Y=2 ^-(Ct)

Formula 3

Expression=Y of SVCT2 _(SVCT2)/ Y _(GAPDH)

The comparative evaluation of expression will be tested the expression of SVCT2 of matched group that material do not have an interpolation as 1 and compare by relative value.The results are shown in Fig. 4.Its result as can be known, the promotion of the expression of SVCT2 depends on the concentration of grapefruit extract.Consider error by the instrumentation value generation of Ct value on the principle of PCR, and then among the embodiment 3 SVCT to produce facilitation effect be thereby that plant extract more than 1.4 times generates in the inhibition test to add simultaneously to compare separately with vitamin C with vitamin C by the melanocyte in embodiment 4 and has the potentiation that melanocyte suppresses effect, therefore, the expression of SVCT is compared the material that increases more than 1.4 times with matched group, be judged as the SVCT facilitation effect.Its result in murine melanoma B16-F10 cell, has the effect that promotes that SVCT2 expresses as can be known more than the grapefruit extract 18 μ g/ml.

Embodiment 8

(generation of the vitamin C transporter of chemical compound (SVCT) promotes active mensuration)

The culture dish that the Caco2 cell (Dainippon Pharmaceutical Co., Ltd's system) that is derived from human colorectal cancer is planted diameter 3.5cm makes and to become 5 * 10 ³Cells is in containing the DMEM culture fluid of 10% inactivated fetal bovine serum (FBS), cultivate in the presence of 37 ℃, 5% carbon dioxide.Cultivate after 3 days, be replaced as new culture medium and add as the stevioside of being tested material (CAS:57817-89-7), geranyl acetate (CAS:105-87-3), nerolidol (CAS:7212-44-4), dihydro jasmone (CAS:1128-08-01), marrubilin (CAS:465-92-9), verbenalin (CAS:548-37-8), fraxin (CAS:524-30-1)), bergaptol (CAS:486-60-2), make that the ultimate density in the culture medium is 100 μ M.Used TRIZOL reagent to reclaim full RNA after 6 hours from testing the material interpolation by conventional method.The full RNA that reclaims supplies in real-time quantitative one stepRT-PCR.According to QuantiTectSYBR Green PCR Kit (キ ア ゲ Application society system) modulation template, use PCR device (MJ リ サ one チ society system) to measure the expression activity that promotes SVCT1, SVCT2 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA.PCR with primer use with embodiment 2 in the same material of material that uses.The PCR condition is as follows.

The reaction condition of＜PCR 〉

About SVCT1 and SVCT2

With (i) 50 ℃, 30 minutes → (ii) 95 ℃, 15 minutes → (iii) 94 ℃, 15 seconds → (iv) 55 ℃, 30 seconds → (v) 72 ℃, 30 seconds is 1 circulation, so repeat 40 times (ii)～(v), preserve down at 4 ℃.

About GAPDH

(i) 50 ℃, 30 minutes → (ii) 95 ℃, 15 minutes → (iii) 94 ℃, 15 seconds → (iv) 58 ℃, 30 seconds → (v) 72 ℃, 30 seconds be 1 circulation, so repeat 35 times (ii)～(iv) after, preserve down at 4 ℃.

The correction of PCR primer is confirmed by the solubility curve of PCR product and the electrophoresis of RT-PCR product, is carried out the comparison of the expression of genes of interest by calculation method similarly to Example 7.The results are shown in table 8.Similarly to Example 7, to compare with matched group has increased when the material conduct of the expression of SVCT has the SVCT facilitation effect more than 1.4 times, the chemical compound that only promotes SVCT1 to express is stevioside, geranyl acetate, dihydro jasmone, verbenalin, fraxin, bergaptol, and the chemical compound that only promotes SVCT2 to express is a marrubilin.Nerolidol promotes the two expression of SVCT1, SVCT2 as can be known.

Table 8

Embodiment 9

(using the generation of the vitamin C transporter (SVCT) of the chemical compound of three-dimensional hepatocyte model to promote active mensuration)

In order in approaching biological intravital environment, to estimate, use in doughnut human three-dimensional hepatocyte model TESTLIVER with the human hepatocytes dimensional culture ^TM(society's system is spun by Japan).Human three-dimensional hepatocyte model is in 12 orifice plates in the human hepatocytes serum-free medium (society's system is spun by Japan), in the presence of 37 ℃, 5% carbon dioxide, carry out shaken cultivation on the vibrating machine of setting.After the shaken cultivation 3 days, be replaced as new culture medium and interpolation as the stevioside of being tested material, geranyl acetate, nerolidol, dihydro jasmone, marrubilin, verbenalin, fraxin, bergaptol, make that the ultimate density in the culture medium is 100 μ M.Per exchange of carrying out culture medium in 24 hours all will be added at every turn and be tested material.Used TRIZOL reagent to reclaim full RNA after 3 days from testing the material interpolation by conventional method.Use the expression evaluation of the PCR in real time of SVCT2 and GAPDH to implement by evaluation methodology similarly to Example 8.The results are shown in table 9.

Similarly to Example 7, to compare the material that increases the expression of SVCT more than 1.4 times with matched group when having the SVCT facilitation effect, the chemical compound that only promotes SVCT1 to express is geranyl acetate, verbenalin, and the chemical compound that only promotes SVCT2 to express is a nerolidol.Promote that as can be known both chemical compounds of expression of SVCT1, SVCT2 are stevioside, dihydro jasmone, marrubilin, fraxin, bergaptol.

Table 9

Embodiment 10

(melanocyte of three-dimensional skin model generates inhibition test (Fontana-Masson dyeing))

Among the three-dimensional skin model MelanoDerm (Network ラ ボ ウ society system), melanocyte when adding grapefruit extract and vitamin C simultaneously generates and suppresses Evaluation on effect and melanocytic morphologic observation, carries out the dyeing by the painted melanocyte of Fontana-Masson.In addition, for having that the activity that makes alpha-glucosidase rises and also estimating when the Echinacea Species extract that 2-O-α-D-glycopyranosyl-ascorbic acid is transformed to the effect of active form L-ascorbic acid rapidly is used in combination.Three-dimensional skin model begins to cultivate according to the explanation of test kit, and culture medium and tested material and be exchanged for new material in per 2 days is tested material same be used in combination of material to use with embodiment 5.Wherein, grapefruit extract uses the extract that uses among the embodiment 7.Cultivate and carried out morphologic observation by microscope on the 10th day, behind PBS washing tissue, carry out Fontana-Masson dyeing by conventional method.The results are shown in Fig. 5,6.

Its result as can be known, separately and during both combinations of the Echinacea Species extract (ECE) of the grapefruit extract of 30 μ g/mL (GFE), 6 μ g/mL, the melanocyte activation, but by adding 2-O-α-D-glycopyranosyl-ascorbic acid (AA2G) of 2 quality %, then melanocyte shrinks, loses activity.And then as can be known, by the grapefruit extract (GFE) that is used in combination 30 μ g/mL, melanocyte produces the inhibition activity and obtains promoting.In addition as can be known, these three kinds of the Echinacea Species extracts (ECE) of the 2-O-α-D-glycopyranosyl-ascorbic acid (AA2G) by being used in combination 2 quality %, the grapefruit extract (GFE) of 30 μ g/mL, 6 μ g/mL, melanocyte generate that to suppress activity the highest.Grapefruit extract (GFE) uses the grapefruit extracting solution of record among the embodiment 7 to add.The Echinacea Species extract uses the Echinacea Species extracting solution of record among the embodiment 5 to add.

Embodiment 11

(mensuration of intake in the ascorbic cell)

The culture dish that murine melanoma B16-F10 cell and the Caco2 cell that is derived from human colorectal cancer are planted diameter 10cm respectively makes and becomes 1 * 10 ⁶Cells is in containing the DMEM culture fluid of 10% inactivated fetal bovine serum (FBS), cultivate in the presence of 37 ℃, 5% carbon dioxide.The cell that murine melanoma B16-F10 cell will be cultivated after 3 days is taken in the cell of test usefulness as vitamin C, and the cell that the Caco2 cell that is derived from human colorectal cancer will be cultivated after 1 week is taken in the cell of testing usefulness as vitamin C.The grapefruit extracting solution that uses embodiment 7 to be put down in writing adds grapefruit extract and makes ultimate density in the culture fluid be 30 μ g/ml and the cell after 24 hours is used for vitamin C takes in test.Culture fluid is being cultivated with buffer (15mM Hepes, 135mM NaCl, 5mM KCl, 1.8mM CaCl ₂, 0.8mM MgCl ₂) in carry out 2 washings, in the presence of 37 ℃, 5% carbon dioxide, carry out pre-cultivation in 1 hour then.Be replaced as new cultivation with buffer, add ascorbic acid (シ グ マ society system) 5mM, murine melanoma B16-F10 cell culture 3 hours, be derived from the Caco2 cell culture 1 hour of human colorectal cancer as vitamin C, with ice-cold PBS washing 3 times, reaction is stopped then.Stripping ascorbic acid in the 70%MeOH1ml that comprises 1mM EDTA, add 10mM dithiothreitol, DTT (Wako Pure Chemical Industries, Ltd.'s system) then as Reducing agent reaction 10 minutes, be transformed into the reduced form ascorbic acid, will be used for analyzing with the filtering material of 0.2 μ m filter (ア De バ Application テ Star Network society system).Analyze and use high performance liquid chromatograph (HPLC), post uses L-post ODS, and (4.6mm * 250mm) (chemical substance evaluation study mechanism system), mobile phase are used A phase (10mM TBAH solution/10mM potassium dihydrogen phosphate/0.5% methanol PH=6) and B (50% methanol) mutually.As elution requirement, apply 0～15 minute only A phase, 15～25 minutes from A to B phase, 25～35 minutes gradients from B to A phase, 35～60 minutes A phases only.The elution time of the reduced form ascorbic acid under this condition is 21 minutes.From the peak heights of this moment, use standard curve to calculate intracellular ascorbic acid concentrations, as intake in the ascorbic cell.Use cell to use Protein Extraction reagent (PIERCE society system) simultaneously from cell extraction protein.By using the albumen quality of using Lowry method instrumentation as the DC Protein Assay (BIO-RAD) of quantification of protein test kit, ascorbic acid is carried out revisal to intracellular intake, represent with ascorbic acid amount (μ mol)/albumen quality (mg protein).The results are shown in Fig. 7～10.Its result as can be known, by adding 30 μ g/ml grapefruit extract, vitamin C increases to about 2.4 times, is being derived from the Caco2 cell of human colorectal cancer and increases to about 1.5 times to intracellular intake in murine melanoma B16-F10 cell.

Embodiment 12

(quantitative analysis of the bergaptol in the grapefruit extract)

Concentration by the contained bergaptol of the grapefruit extract (solid constituent among the ball Off ア Le コ ス system grapefruit fruit extract Off ア Le コ レ Star Network ス grapefruit B) that uses among the HPLC method analysis embodiment 6,7,10,11.Its result, the bergaptol in the grapefruit extract is 0.015 quality %.

The HPLC analysis condition

(post: Capcell pak Cl84.6mm φ * 250mm (SHISEIDO)

Column temperature: 40 ℃

Detector: UV 315nm

Eluent: (A) 5% aqueous acetic acid: (B) acetonitrile=75:25

Flow velocity: 1.0mL/ minute,

Injection rate: 20 μ L,

Sample retarder thinner: DMSO/ ethanol=2:18 (V/V)).

Bergaptol dissolution time: 9.6 minutes

Embodiment 13

(melanocyte of chemical compound group generates inhibition test)

To be used in combination the chemical compound that obtains among the present invention and vitamin C effect in order estimating,, to measure melanocyte and generate and suppress active by following test to whitening function.

As the cell that generates melanocyte, use the B16-F10 melanoma cell that is derived from mice, in each holes of 6 orifice plates according to 1 * 10 ⁵The cells sowing was cultivated 24 hours in the culture fluid (ギ Block コ society system) of DMEM+10%FBS, made cytoadherence.Thereafter, [Nle4, the D-Phe7] α-MSH (シ グ マ ア Le De リ Star チ society system) with DMEM+10%FBS replaces culture fluid, interpolation 100nM induces melanocyte to produce.Simultaneously, as being tested material, add ugaferine25 μ M (containing 0.1%DMSO), marrubilin, lapiferine50 μ M (containing 0.1%DMSO), fraxin 100 μ M (containing 0.1%DMSO), contrast interpolation 0.1%DMSO.Tested material and organized separately, carry out the melanocyte generation and suppress to estimate cultivation in 48 hours thereafter.Tested material and added vitamin C (シ グ マ ア Le De リ Star チ society system) to ultimate density 4mM at per 24 hours thereafter, added 2 times (amount to and add 8mM), carry out the melanocyte generation and suppress to estimate with the ascorbic group that is used in combination.

The mensuration of melanocyte amount is undertaken by following: by trypsin treatment cell is peeled off, repeated 2 PBS washings, with the cell solubilising, absorbance is 475nm by 1%TritonX (with the pure medicine of light society system).Use synthetic melanocyte (シ グ マ ア Le De リ Star チ society system) to make standard curve and calculate the melanocyte amount.The melanocyte generation suppression ratio of effect by following formula 4 that be used in combination of being tested that material and vitamin C bring calculates.

(formula 4)

Be used in combination melanocyte that effect brings and generate suppression ratio (%)=(the melanocyte amount/quilt when the 1-vitamin C is used in combination is tested the melanocyte amount of material when independent) * 100

Wherein, melanocyte amount when the quilt of contrast is tested the melanocyte value of material when independent and is meant the dissolved solvent that is used for being tested material that adds ultimate density 0.1%, DMSO, the melanocyte value the when vitamin C of contrast is used in combination is meant the melanocyte amount when being used in combination 0.1%DMSO and vitamin C.In addition, the generation suppression ratio that effect brings that is used in combination of contrast is meant: the melanocyte amount when 0.1%DMSO is used in combination melanocyte amount when adding with respect to independent interpolation 0.1%DMSO with vitamin C generates the value that suppression ratio is represented as melanocyte.

The results are shown in table 10.When 0.1%DMSO is used in combination interpolation with vitamin C, it is 61.4% that the melanocyte of the melanocyte amount when adding separately with respect to 0.1%DMSO generates suppression ratio, when marrubilin, ugaferine, lapiferine, fraxin and vitamin C were used in combination, melanocyte generated suppression ratio and is increased to 69.7%, 64.6%, 68.1%, 68.4% respectively.This is to be used in combination the data of interpolation and marrubilin, ugaferine, lapiferine, the independent unpredictable significant synergy of data of fraxin with vitamin C from 0.1%DMSO.

This synergy can be speculated as and be produced by the SVCT of marrubilin, ugaferine, lapiferine, fraxin that facilitation effect brings.

Table 10

The melanocyte of marrubilin, ugaferine, lapiferine, fraxin generates inhibition test

Embodiment 14

(collagen of chemical compound group produces evaluation test)

For the chemical compound of estimating about obtaining among the present invention produces being used in combination effect of ability (being also referred to as the synthetic promotion ability of collagen) and carries out following test with ascorbic collagen, produce facilitation thereby measure collagen.Be derived from normal human subject skin the fibrous bud cell, be that NHDF cell (Cambrex Bio ScienceWalkersville society system) is planted 24 hole porous plates (Off ア Le コ Application society system) and made and to become 5 * 10 ⁴Cells/well in the culture fluid (ギ Block コ society system) of DMEM+10%FBS, cultivated 3 days in the presence of 37 ℃, 5% carbon dioxide.Be replaced as the culture fluid (the pure medicine of three light commercial firm system) of FBM (serumfree, serum-free), added marrubilin 50 μ M, stevioside, bergaptol 100 μ M as testing material, 0.1%DMSO is added in contrast, carries out 24 hours pre-cultivation.After pre-the cultivation, with FBM culture fluid washed cell, be replaced as the FBM culture fluid after, vitamin C (シ グ マ ア Le De リ Star チ society system) added to making in the culture fluid that ultimate density is 10 μ M, handled 1 hour, vitamin C is taken in the cell., with FBM culture fluid washed cell, be replaced as FBM culture fluid, after 24 hours, the conditioned medium (conditioned medium (CM)) of 1ml all reclaimed in each hole, make the sample that type i collagen is measured thereafter.For the CM of aforementioned recovery, measure collagen quantity (detectable limit concentration: 10ng/ml) with typel collagenC-peptide (PIP) EIA kit ( カ ラ society system).The anti-PIP monoclonal antibody 100 μ l that add peroxidase labelling in each hole of anti-PIP monoclonal antibody plate (96 hole) then, add and dilute each 20 μ l of CM of 20 times in advance with the PBS that contains 1% bovine serum albumin, cultivate 3 hours at 37 ℃., lose reactant liquor, after 200 μ l PBS washing 4 times, add each 100 μ l substrate TMBZ (3,3 ', 5,5 '-tetramethyl benzidine), at room temperature make its reaction 15 minutes thereafter.Add 1N sulphuric acid 100 μ l when reaction finishes, measure the absorbance of wavelength 450mm.

The measurement result of marrubilin is shown in table 11.Marrubilin has seen compared with the control that when vitamin C is used in combination collagen produces facilitation.

Stevioside, bergaptol the results are shown in table 12.Stevioside, bergaptol have seen compared with the control that when vitamin C is used in combination collagen produces facilitation.

Table 11

The collagen of marrubilin produces facilitation

	Tested material (ng collagen/10 when independent 5cells)	(ng collagen/10 when vitamin C is used in combination 5cells)	Ratio (when vitamin C is used in combination/tested material when independent)
				Contrast (0.1%DMSO)	1440	2242	1.56
Marrubilin (50 μ M)	1510	2567	1.70

Table 12

The collagen of stevioside, bergaptol produces facilitation

	Tested material (ng collagen/10 when independent 5cells)	(ng collagen/10 when vitamin C is used in combination 5cells)	Ratio (when vitamin C is used in combination/tested material when independent)
				Contrast (0.1%DMSO)	1610	2361	1.47
Stevioside (100 μ M)	1553	2724	1.75
				Bergaptol (100 μ M)	1714	2660	1.55

Formulation Example 1

[manufacturing of tablet]

Use Herba Houttuyniae extract and vitamin C, make the tablet of following composition by conventional method.

(composition) (prescription: weight %)

Herba Houttuyniae extract 14.39

Folium olive extracts hydrolysate 4.66

Cystine 16.00

Nicotiamide 1.2

Vitamin C 24.45

Cellulose 26.80

Starch 4.00

Edible egg-shell meal 6.00

Sucrose ester 2.50

Formulation Example 2

[manufacturing of fruit juice]

Use contains the Flos Chrysanthemi extract and the vitamin C of stevioside, makes the fruit juice of following composition by conventional method.

(composition) (prescription: weight %)

Fructose Glucose Liquid sugar 15.00

Citric acid 10.40

Vitamin C 0.50

Spice 0.02

Pigment 0.10

Flos Chrysanthemi extract 10.00

Purified water 63.98

Formulation Example 3

[manufacturing of ointment]

Use marrubilin and vitamin C, make the ointment of following composition by conventional method.

(composition) (prescription: weight %)

White vaseline 24.00

Propylene glycol 12.00

Stearyl alcohol 20.00

Methyl parahydroxybenzoate 0.05

Vitamin C 2.00

Marrubilin 2.00

Spice 0.10

Purified water 39.85

Formulation Example 4

[manufacturing of astringent]

Use grapefruit extract, 2-O-α-D-glycopyranosyl-ascorbic acid (AA2G), Echinacea Species extract, make the astringent of following composition by conventional method.

(composition) (prescription: weight %)

1,3 butylene glycol 5.00

Ethanol 4.50

Glycerol 10.00

Echinacea Species extract 0.50

AA2G 2.00

Grapefruit extract 0.50

Dipropylene glycol 2.00

Glycyrrhizic acid dipotassium salt 0.10

Potassium hydroxide 0.40

Purified water 75.0

Formulation Example 5

[manufacturing of facial film]

Use grapefruit extract, 2-O-α-D-glycopyranosyl-ascorbic acid (AA2G), Echinacea Species extract, make the facial film of following composition by conventional method.

(composition) (prescription: weight %)

1,3 butylene glycol 6.00

Ethanol 3.50

Glycerol 5.00

AA2G 2.00

Fungi plant glue (sclerotium gum) 0.30

Folium olive extracts hydrolysate 1.00

Echinacea Species extract 0.20

Sodium citrate 0.20

Citric acid 0.02

Grapefruit extract 0.5

Cystine 0.08

Carboxymethyl cellulose 0.30

Potassium hydroxide 0.40

Purified water 80.50

Formulation Example 6

[manufacturing of emulsion]

Use grapefruit extract, 2-O-α-D-glycopyranosyl-ascorbic acid (AA2G), Echinacea Species extract, make the emulsion of following composition by conventional method.

(composition) (prescription: weight %)

AA2G 2.00

Potassium hydroxide 0.38

Glycyrrhizic acid dipotassium salt 0.10

Xanthan gum 0.14

Alcaligenes produces polysaccharide body 0.07

Glycerol 2.00

Propylene Glycol 3.00

1,2-pentanediol 2.00

Lecithin 1.00

Batilol 0.10

Dimethicone 1.50

Ethanol 4.50

Echinacea Species extract 0.20

Jojoba leaf extract 0.10

Folium olive extracts hydrolysate 1.00

Grapefruit extract 0.50

Cystine 0.01

Citric acid 0.02

Sodium citrate 0.15

Purified water 81.23

Formulation Example 7

[manufacturing of astringent]

Use geranyl acetate, nerolidol, dihydro jasmone, bergaptol, 2-O-α-D-glycopyranosyl-ascorbic acid (AA2G), Echinacea Species extract, make the astringent of following composition by conventional method.

(composition) (prescription: weight %)

1,3 butylene glycol 5.00

Ethanol 4.50

Glycerol 10.00

Echinacea Species extract 0.50

AA2G 2.00

Geranyl acetate 0.01

Nerolidol 0.01

Dihydro jasmone 0.01

Bergaptol 0.01

Dipropylene glycol 2.00

Glycyrrhizic acid dipotassium salt 0.10

Potassium hydroxide 0.40

The purified water residue

Industrial utilizability

According to agent for promoting vitamin C transporter production of the present invention, in the analysis of using cell, can significantly promote the expression of the mRNA of SVCT1 and/or SVCT2, it can be used as skin-whitening composition or the synthetic composition for promoting of collagen.

Sequence table

＜110〉FANCL CORPORATION (FANCL Corporation)

＜120〉agent for promoting vitamin C transporter production

<130>PC27-027

10:4 120088-14

<150>JP 2006-036923

<151>2006-2-14

<150>JP 2006-244344

<151>2006-9-8

<150>JP 2006-332214

<151>2006-12-8

<160>8

<210>1

<211>20

<212>DNA

＜213〉artificial sequence

<400>1

<210>2

<211>20

<212>DNA

＜213〉artificial sequence

<400>2

<210>3

<211>20

<212>DNA

＜213〉artificial sequence

<400>3

<210>4

<211>20

<212>DNA

＜213〉artificial sequence

<400>4

<210>5

<211>20

<212>DNA

＜213〉artificial sequence

<400>5

<210>6

<211>20

<212>DNA

＜213〉artificial sequence

<400>6

<210>7

<211>20

<212>DNA

＜213〉artificial sequence

<400>7

<210>8

<211>20

<212>DNA

＜213〉artificial sequence

<400>8

Claims (13)

1. agent for promoting vitamin C transporter production, it is characterized in that, contain more than at least one that is selected from the chemical compound group that marrubilin, cholesteryl benzoate, ugaferine, lapiferine, geranyl acetate, nerolidol, dihydro jasmone, Carvacryl acetate, Kessazulen, dihydroconiferyl alcohol, stevioside, DL-australene, verbenalin, 1-heptacosanol, 4 hydroxy coumarin, cholic acid, cholesteryl oleate, fraxin, bergaptol form.

2. agent for promoting vitamin C transporter production, it comprises the hydrolysate of ground product, its extract or the extract of the plant that contains right and require 1 described arbitrary chemical compound, animal, mineral, microorganism.

3. vitamin C absorbs composition for promoting, and it combines claim 1 or 2 described agent for promoting vitamin C transporter production and vitamin C or vitamin C derivatives.

4. vitamin C according to claim 3 absorbs composition for promoting, and vitamin C derivatives is 2-O-α-D-glycopyranosyl-ascorbic acid.

5. whiten and use compositions, it comprises claim 3 or 4 described vitamin Cs absorb composition for promoting.

6. collagen synthesizes composition for promoting, and it comprises claim 3 or 4 described vitamin Cs absorb composition for promoting.

7. agent for promoting vitamin C transporter production, it is characterized in that, contain the hydrolysate of above ground product, its extract or extract of at least one that is selected from the group that somewhat-white magnolia Drymotaenium miyoshianum (Mak.) Mak., Semen Armeniacae Amarum, sweet tea, grapefruit, Radix Ophiopogonis, Caulis Hederae Sinensis, Saponaria officinalis, Herba Houttuyniae, Flos Matricariae chamomillae, gambir, Radix Hamamelidis Mollis, Mulberry, Testa oryzae, Herba Equiseti Arvinsis form.

8. vitamin C absorbs composition for promoting, it is characterized in that, the described agent for promoting vitamin C transporter production of claim 7 and vitamin C or vitamin C derivatives are combined.

9. vitamin C according to claim 8 absorbs composition for promoting, and vitamin C derivatives is 2-O-α-D-glycopyranosyl-ascorbic acid.

10. whiten and use compositions, it is characterized in that, it comprises claim 8 or 9 described vitamin Cs absorb composition for promoting.

11. collagen synthesizes composition for promoting, it is characterized in that, it comprises claim 8 or 9 described vitamin Cs absorb composition for promoting.

Use compositions 12. whiten, it is characterized in that, it contains claim 3,4,8 or 9 described vitamin Cs absorb composition for promoting and glucosidase agent.

13. according to claim 12 whitening used compositions, the glucosidase agent is to be selected from least one in the group that Echinacea Species, yeast, bacillus bifidus, lactobacillus form above ground product, its extract or to extract hydrolysate.

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