KR100822835B1 - Plant extracts and cosmetic compositions for improvement of scalp and hair - Google Patents

Abstract

An extract of a mixture of herb medicines is provided to show excellent hair growth, hair loss preventing effect, antibacterial, anti-inflammatory, anti-itching, and scalp and hair moisturizing effect without inducing any side effect such as skin irritation. A cosmetic composition for scalp and hair comprises an extract of a mixture of herb medicines consisting of 1-5 parts by weight of Angelica gigantis radix, 1-5 parts by weight of Psoralea corylifolia, 1-5 parts by weight of Thuja Folium et Ramulus, 1-5 parts by weight of Eclipta prostrata, 0.5-6 parts by weight of Lycium chinenses Mill., 0.5-6 parts by weight of Rubus Coreanus, 0.5-6 parts by weight of Mori Cotex Radicis, 0.5-6 parts by weight of Rehmanniae Radix Preparata, 0.5-6 parts by weight of Ligustrum japonicum, 0.5-6 parts by weight of Polygonum multiflorum, 0.5-6 parts by weight of black sesame, 1-20 parts by weight of Sophora flavescens, 1-20 parts by weight of Angelica dahurica, 1-20 parts by weight of Leonurus sibiricus, 0.5-4 parts by weight of Salvia miltiorrhiza, 0.5-4 parts by weight of Persicae Semen, 0.5-4 parts by weight of myrrh, 0.5-4 parts by weight of Chrysanthemum indicum, 0.5-4 parts by weight of frankincense, 0.5-4 parts by weight of ginseng, 0.5-4 parts by weight of Cnidium officinale, 0.5-4 parts by weight of Albizzia julibrissin and 0.5-4 parts by weight of Corydalis turtschaninovii, wherein the extract is obtained by using a solvent such as water, ethanol, glycerin, butylene glycol and propylene glycol. Further, the composition is a form selected from a group consisting of lotion, cream and shampoo.

Description

Plant extracts and cosmetic compositions for improvement of scalp and hair}

The present invention relates to an herbal extract for improving the scalp and hair and scalp and hair improving composition using the same, more specifically, a medicine having the effect of preventing hair loss and hair loss, a medicine having the effect of preventing baldness, having a soothing effect It relates to herbal medicine extracts, including herbal medicines, such as to improve the blood circulation and hypoallergenic scalp and hair improvement cosmetic composition using the same.

Generally, the causes of hair loss include the effects of male hormones, decreased metabolic function in the hair roots due to poor blood circulation, malnutrition, side effects from drugs, damage to the scalp by dandruff or bacteria, and genetic factors. Known. In addition, the scalp and hair due to excessive shampoo, drying, hair styling and the like and the natural moisturizing component of the scalp by the chemical cleansing ingredients are removed to damage the scalp and hair. In particular, in recent years, a growing number of people who suffer from hair loss and hair damage due to excessive stress due to changes in the social environment, etc., and the age and gender restrictions and boundaries are also expanding.

Therefore, researches on materials and ingredients that can improve the condition of the scalp and hair, including prevention of hair loss, have been actively conducted, and as such materials and ingredients, Korean Patent Nos. 10-0038819, 10-0026222, and 10- As introduced in 0002207, 10-0046064, etc., anti-male hormones, anti-seborrheic agents, keratin-dissolving agents and removing agents, dandruff disinfectants and amino acids, vitamins, and the like have been used. However, these existing materials and ingredients are not excellent in hair loss prevention and hair improvement effect, it is pointed out that there is a limit to the content as a problem for safety and skin irritation.

Unlike direct pharmacological prescriptions such as chemicals, traditional Chinese medicine has traditionally been associated with the improvement of scalp and hair, and the enhancement of hepatic and renal function associated with the skin, increased blood flow and blood circulation in the scalp due to improved blood and blood circulation. The focus has been on indirect treatment and prevention through herbal remedies that can lead to the development of medicinal products.

Recently, as scientific evaluation methods have been studied, hair growth effect, wool effect, antibacterial effect, skin of herbal extracts such as Korean Patent Registration Nos. 10-0298647, 10-0524291, 10-0534483, 10-0509910, etc. With scientific verification of soothing effects, various scalp and hair improvement herbal extracts that can compensate for the shortcomings of the chemical preparations have been developed. However, the need for scalp and hair cosmetic compositions that are all excellent and hypoallergenic in terms of safety are still steadily being raised, such as anti-hair and hair loss effect, hair growth and wool effect, anti-itch effect, moisturizing effect, and crack prevention effect. have.

In the present invention, excellent hair growth and hair loss prevention effect, hair growth and wool effect, antibacterial effect against dandruff, without causing skin irritation, scalp and hair damage caused by the removal of natural moisturizing ingredients, which are disadvantages of existing scalp and hair improvement composition, It is an object of the present invention to provide an herbal extract having an anti-itch effect, moisturizing effect, hair flexibility improvement effect and anti-cracking effect and a composition containing the same.

In order to achieve the above object of the present invention, the present invention is Angelica, Bogolji, baekbaekye, nasturtium, wolfberry, Bokbunja, Sangbaekpi, Sukjiwang, Jojeongsil, Drip Shou, Black Jim, Gosam, White Paper, Motherwort, Dansam, Doin, Myrrh Provides a composition for scalp and hair, characterized in that it comprises a mixed herbal extract of gamguk, frankincense, ginseng, cheongung, haphwanpi and Hyunho color.

More preferably, the mixed herbal medicine in the composition is 1 to 5 parts by weight, respectively, Angelica, golgol, baekryeop and nasturtium; 0.5 to 6 parts by weight of Gugija, Bokbunja, Sangbaekpi, Sukjihwang, Journey, Red Sewage, and Black Horse; 1 to 20 parts by weight of red ginseng, white paper and motherwort, respectively; And it provides a composition for scalp and hair, characterized in that it contains 0.5 to 4 parts by weight, respectively, Dansam, Doin, myrrh, persimmon soup, frankincense, ginseng, Cheongung, Haphwanpi and Hyunho colors.

The cosmetic composition according to the present invention is excellent in hair growth and hair loss preventing effect, wool effect, antibacterial effect, anti-itch effect, and moisturizing effect of the scalp and hair and does not cause side effects such as skin irritation.

Hereinafter, the herbal extract for improving scalp and hair and the cosmetic composition using the same according to the present invention will be described in detail.

First, the components of the herbal extracts contained in the cosmetic composition according to the present invention will be described in more detail.

Angelica gigas Nakai of the herbal medicine according to the present invention contains a lot of coumarin in the root and outpost. The main constituents of coumarin are known as decursin, decursinol, ostol, umbellininine and the like. Root also contains essential oils, resins, etc., and is known as an excellent medicine for improving blood circulation, antibacterial action and anti-inflammatory action.

Among the herbal medicines according to the present invention, golgol ( Psoralea corylifolia Linne) contains a resin, essential oil, bakuchiol, psoralen, angelicin, bavachin, isobavachin, etc., the shell contains psoralidin, corylifolin as the main ingredients and improves kidney function It is known as an excellent anti-inflammatory and analgesic medicine.

Cheukbaekyeop (Biota orientalis Endllcher) of saengyakjae according to the invention comprises a juniperic acid, thujone, mayurone, sabinic acid, etc. as a main component, and there is known to be excellent medicines such as anti-inflammatory action, antibacterial action.

Among the herbal medicines according to the present invention, Eclipta prostrata Linne includes nicotine, anabasine, acacetin-7-O-rutinoside, 3-O-angeloylhamaudol, 2-methylpropanoyl, kalopanax saponin, acertannin, vitamin A, thiophene, etc. It is known as an excellent hemostatic effect.

Wolfberry ( Lycium) among herbal medicines according to the present invention chinense Miller) contains lipids, proteins, sugars, fibers and carotenoids, zeaxanthin, 18 amino acids, polysaccharides, pisalien, scopoline, etc. It is known as this excellent medicine.

Bokbunja ( Rubus) among herbal medicines according to the present invention coreanus Miquel) contains coreanogenoic acid, coreanoside F1, nigaichigoside F1, suavissimoside R1, essential oils, Saccharide and vitamin C as main ingredients, and is known as an excellent anti-oxidant agent for antipyretic, cardiovascular, diuretic and skin cell damage have.

Morus alba in herbal medicine according to the present invention alba Linne) contains umberrin, umberlobogen, cyclomulberin, and coumarin derivatives such as scopoline, umbeliferon, and triterpenoids such as amiline and betulinic acid. It is known as an excellent medicine for action, anti-inflammatory action, antioxidant effect.

Sukjiwang ( Rehmannia) among herbal medicines according to the present invention glutinosa Liboschitz var . purpurea Makino) contains iridoid glycosides such as catalpol and lehmanine, as well as mannitol, stakiose, glucose, and amino acids as its main ingredients. .

Among the herbal medicines according to the present invention ( Ligustrum lucidum Aiton) contains d-mannit, oleanolic acid, oleic acid, linoleic acid and the like as the main ingredients to protect the liver and kidneys, black hair and is known as an excellent anti-inflammatory effect.

Drip Sewage ( Polygonum) among herbal medicines according to the present invention multiflorum Thunberg) contains polyoxypregnan glycosides, contains Siberia coside D glycosides, sugars, starches, fats, etc. as main ingredients, and is known as an excellent medicinal herb.

Among the herbal medicines according to the present invention, black jima ( Sesamum indicum Linne) contains fatty oils containing oleic acid, linoleic acid, palmitic acid, etc. as main ingredients, and is known as an effective medicine for anti-inflammatory action and blacking hair while protecting liver and kidney.

Ginseng among herbal medicines according to the present inventionSophora angustifolia Siebold et Zuccarini) contains the main components of matrin, oxymatrine, sophoranol, methylcytizin, anagiline, allomatrine, isohydrokaritin, nor-anhydroikaritin, and anti-inflammatory, antifungal and antibacterial activity And it is known as an excellent drug for antiallergic action.

White paper of herbal medicine according to the present invention dahurica Bentham et Hooker) is a coumarin derivative, which contains imperatorin, pelopterin, xanthotoxin, biaxangelacin, biaxangelicol, and essential oils as main ingredients, and has anti-inflammatory and anti-allergic properties. Known.

Motherwort ( Leonurus) among herbal medicines according to the present invention sibiricus Linne) contains leonurine, stachydrine, leonuridine, rutin, benzoic acid as main ingredients, and is known as an excellent drug for diuretic and blood circulation improvement.

Salvia miltiorrhiza Bunge of the herbal medicine according to the present invention contains tanshinone, dihydrotanshinone, cryptotanshinone, methyl tanshinonate, methylene tanshiquinone, beta-sitosterol as main ingredients, and improves blood circulation, removes blood, anti-inflammatory and antibacterial drugs. Known.

Among the herbal medicines according to the present invention ( Prunus persica Batsch) contains amigdaline, emulsine, oleic acid, linoleic acid, palmitic acid and cytosterol as main ingredients and is known for its excellent blood circulation, anti-inflammatory and skin moisturizing effects.

Myrrh of the herbal medicine according to the present invention ( Commyphora molmol Engler) contains resins, essential oils, handing, terpene, sesquiterpene, cinnamic aldehyde as the main ingredients, and is known as an excellent medicine for improving blood circulation, anti-inflammatory, sterilization and disinfection.

Chrysanthemum indicum Linne of the herbal medicine according to the present invention contains essential oils such as Borneol, Bornyl Aceteatel Campa, Tetracozan, Hexacozan and adenine, Choline, Vitamin A, Vitamin B1, etc. It is known as an excellent medicine for lowering blood pressure, anti-inflammatory, antibacterial action.

Frankincense ( Boswellia carterii Birdwood) of the herbal medicine according to the present invention contains alpha-boswellic acid, beta-boswellic acid, olibanorescene, beta-pinene, dipentene as the main ingredients and is known as an excellent anti-inflammatory and anti-inflammatory drugs.

Ginseng ( Panax) among herbal medicines according to the present invention ginseng CA Meyer) is a saponin glycoside, which is panaxanadiol, panaxanthol, beta-cysterol, oleanoic acid, and panacene, panaxinol, camphorol, tripolim, panacenoids and vitamins, nicotinic acid, pantothenic acid, folic acid, biotin Its main ingredients include antistress, lowering blood pressure, improving blood circulation, and tonic are known as excellent medicines.

Cheongung of the herbal medicine according to the present invention (Cnidium officinale Makino) contains major ingredients such as condylide, neocidelide, ligustilide, knidium acid, knidium acid ether, sedananoic acid, coumarin, and mannitol, and has excellent blood circulation and antibacterial effects. Known.

The herbal medicine Albizzia julibrissin Durazzini according to the present invention contains albigenic acid, echinocystic acid, acacic acid, quercetin, tirterpenoid, albizzin, vitamin C, acertannin, julibroside, julibrin, etc. It is known as a medicine.

Corydalis ( Corylais) among herbal medicines according to the present invention ternata Nakai) contains corridin, alkaloid, berberine, 1-canadine, protopine, 1-tetrahydrocoptisine as main ingredients, and is known as an excellent medicine for removing blood, improving blood circulation, and sedating.

The mixed herbal medicine extract for improving scalp and hair provided to achieve the object of the present invention is (i) a method of extracting the medicinal herb mixture by dipping the extractant at 20 to 40 ° C, and (ii) the extractant at 60 to 90 ° C. A method of extracting the mixture of the herbal medicines by reflux, (iii) A method of extracting the herbal medicines mixture by extracting ultrasonic waves at 20 to 70 ℃ extraction solvent, (i) Each of the 23 kinds of herbal medicines (i), (ii) Or it may include a method of separately extracting by the method of (iii) and mixing each extract according to a certain ratio, but is not limited thereto.

As the extraction solvent, solvents such as lower alcohols such as water, ethanol, methanol and isopropanol, ethyl acetate and acetone may be used. In addition, polyhydric alcohols such as glycerin, butylene glycol and propylene glycol may also be extracted. It may be included as a solvent. However, as the extraction solvent of the herbal medicine mixture according to the present invention, water, ethanol or a mixed solvent thereof is preferable, and the extraction solvent is extracted by using 90 to 50% by weight relative to the total weight of the extraction solvent and the herbal medicine mixture. desirable.

The scalp and hair composition of the present invention preferably contains 1 to 80% by weight, and more preferably 10 to 50% by weight of the herbal medicine extract that is an ingredient exhibiting the effect according to the present invention.

The cosmetic composition of the present invention is a moisturizer, thickener, surfactant, emulsifier, preservative, antioxidant, alcohol, flavoring, pH adjusting agent, natural within the range that does not impair the effects of the present invention in addition to the herbal extract for the improvement of the scalp and hair. As well as extracts, the scalp and hair improvement components of the chemical components for doubling the scalp and hair improvement effect can be blended in an appropriate amount.

On the other hand, the cosmetic composition prepared by containing the scalp and hair improvement herbal medicine extract may be prepared in a variety of formulations, such as lotions, lotions, creams, shampoos, specifically, emulsion, cream, paste It can be applied in the form and can be prepared using a conventional cosmetic preparation method. In particular, the aforementioned scalp and hair composition is more preferably used as a cosmetic for improving the scalp and hair.

The present invention provides an herbal extract-containing composition excellent in hair growth and hair loss prevention effect, wool effect, antibacterial effect, itching prevention effect, moisturizing effect of the scalp and hair. The composition according to the present invention has been shown to have excellent hair growth and hair loss prevention effect, wool effect, excellent antibacterial and bactericidal effect against dandruff, and safe and excellent feeling when applied to the scalp and hair compared to general hair composition. . Therefore, it has an excellent effect on scalp and hair improvement, and is expected to be able to exert an excellent effect on the commercialization or commercialization of the product as a product for scalp and hair without skin irritation, especially a hair cosmetic product.

Hereinafter, specific examples such as examples, formulation examples, etc. will be described in order to specifically describe the present invention. However, embodiments or formulations according to the present invention can be modified in many different forms, the scope of the invention is not to be construed as limited to the examples described below. Examples and formulations according to the present invention are provided only to more fully explain the present invention to those skilled in the art.

<Example 1 and Comparative Examples 1 to 4> Preparation of the scalp and hair improvement herbal medicine extract

In order to measure the scalp and hair improvement effect of the herbal extracts were prepared herbal extracts as described in Table 1 below. At this time, the unit of component content is weight%. Each composition was mixed in the ratio of the herbal mixture and the extraction solvent in a 1: 5 ratio, and prepared by the ultrasonic extraction method. Comparative Example 1 is a group of medicinal herbs classified as having hair growth and hair loss prevention effect, Comparative Example 2 is a group of medicinal herbs classified as having a baldness efficacy, Comparative Example 3 is a group of medicinal herbs classified as having a skin soothing effect, Comparative Example 4 extracted a group of medicinal herbs classified as having a blood circulation improving effect, Example 1 was extracted by mixing the whole medicinal herbs as a herbal composition of the present invention.

turn Medicinal name Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 One Donkey 0.34 3.0 - - - 2 Golgol 0.34 4.6 - - - 3 Temporal lobe 0.50 4.6 - - - 4 Nasturtium 0.50 4.6 - - - 5 Wolfberry 0.67 - 2.2 - - 6 Bokbunja 1.00 - 3.4 - - 7 White skin 1.00 - 3.4 - - 8 Hungjiwang 0.67 - 2.2 - - 9 Journey Room 0.50 - 1.7 - - 10 Droopy 0.67 - 2.2 - - 11 Don't be black 0.50 - 1.7 - - 12 Gosam 2.69 - - 6.8 - 13 blank 2.02 - - 5.0 - 14 motherwort 2.02 - - 5.0 - 15 Salvia 0.50 - - - 2.5 16 Doin 0.50 - - - 2.5 17 Myrrh 0.34 - - - 1.6 18 Country 0.34 - - - 1.7 19 frankincense 0.34 - - - 1.7 20 Ginseng 0.34 - - - 1.7 21 Cheongung 0.34 - - - 1.7 22 Blood 0.34 - - - 1.7 23 Corydalis 0.34 - - - 1.7 24 Purified water 20.8 20.8 20.8 20.8 20.8 25 ethanol 62.4 62.4 62.4 62.4 62.4

Experimental Example 1 Scalp and Hair Improvement Effect Animal Test

The scalp and hair improvement effects were measured using a hair growth test using C57BL mice, a growth test of hair follicle cells in mouse tissues, and a test for inflammatory cell expression.

Six mice were used for each of the compositions prepared in Table 1, and the C57BL mice used for the measurements were 6 week old of resting hairs with pink dorsal skin color and carefully shaved, mowed 0.2 ml of the Example and Comparative Example samples were sufficiently applied to each silver site using a brush, and then massaged for about 3 minutes, followed by rinsing with distilled water. Sample treatment was performed once daily, and hair growth promoting conditions were observed at 4, 7, 10, and 14 days, respectively, for 2 weeks, when the hairs of the mice were completely covered with hair growth.

Hair follicle expression evaluation and inflammatory cell expression evaluation was carried out as follows. During the hair growth promotion evaluation test, three animals were examined at the end of the 7 days after the start of the test and three at the end of the test, and the sample application sites were dissected and fixed with formalin. After dehydration with alcohol and xylene step by step, embedded with paraffin, 5 μm sections were made using a tissue slicer, paraffin was removed again with alcohol and xylene, and hair follicle expression was evaluated by H & E staining. Histologic changes were observed, and inflammatory cell expression was evaluated by toluidine blue staining and histological changes of inflammatory cells (mast cells) by light microscopy.

Among the results of the test, hair growth promoting evaluation was evaluated according to the evaluation criteria shown in Table 2 below, hair follicle expression evaluation and inflammatory cell expression evaluation was compared with the photograph taken the results observed by optical microscope and the evaluation shown in Table 4 below Evaluation was made based on the criteria. The results of hair growth promoting evaluation are shown in Table 3 and FIG. 1, hair follicle expression evaluation is shown in Table 5 and FIG. 2, and inflammatory cell expression evaluation is shown in Table 6 and FIG. 3.

Hair growth Scoring Judgment 0 to 20% - no effect 20 to 40% ± weak 40 to 60% + Good 60 to 80% ++ Excellent 80% or more +++ Very good

Evaluation date division Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 4 days after application Hair growth 8 8 8 5 8 Scoring - - - - - Judgment no effect no effect no effect no effect no effect 7 days after application Hair growth 10 10 15 15 15 Scoring - - - - - Judgment no effect no effect no effect no effect no effect 10 days after application Hair growth 55 14 32 75 52 Scoring + - ± ++ + Judgment Good no effect weak Excellent Good 14 weeks after application Hair growth 90 25 75 93 80 Scoring +++ ± ++ +++ ++ Judgment Very good weak Excellent Very good Excellent

As shown in Table 3, most of the samples showed no hair growth effect after 7 days of application of the sample, but after 10 days, the hair growth effects of Example 1, Comparative Example 3, and Comparative Example 4 were good. It appeared to appear. In addition, after 14 days, it was confirmed that most of the samples had excellent hair growth effects except for Comparative Example 1. In particular, in Example 1 and Comparative Example 3, the hair growth effect was very excellent. On the other hand, in Comparative Example 1 it was confirmed that the hair growth effect is significantly lower as well as erythema produced by the stimulation. As a result of Comparative Example 1, a group of medicinal herbs known to have a hair regrowth effect, it was possible to confirm the effect of skin stimulation on the hair growth along with the hair regrowth effect, and these results could also be confirmed as a result of Example 1. In the case of Example 1, the herbal medicines included in Comparative Example 1 were included, but the whole herbal extracts were not accompanied by skin irritation, and thus, did not inhibit the hair growth. The hair growth effect of Example 1 and Comparative Examples 1 to 4 can also be confirmed through a photograph of the mouse used for the test as shown in FIG. 1. It was confirmed that the hair growth effect of the herbal extracts of the remaining samples except Comparative Example 1 was superior to the control group did not apply the sample.

As shown in Table 5 and Figure 2, hair follicle expression evaluation, in the case of Comparative Example 1 as in the hair growth effect test results, it was confirmed that the development of hair follicle cells is delayed due to stress caused by skin stimulation, Comparative Example In case of 3, hair follicle cells were partially developed. In particular, in the case of Example 1, the herbal extract of the present invention, it was confirmed that the development of hair follicle cells was very excellent. In the hair growth test, a good result was similarly obtained in Example 1 and Comparative Example 3, but it was confirmed that the expression of hair follicle cells showed the best result in the mouse coated with Example 1. The expression of hair follicle cells is the basis of hair growth, in particular, and can be said to be an important criterion for hair growth effects.

Cell expression Scoring Judgment 0 to 10% - No expression 10 to 30% ± Partial cell expression 30 to 60% + Good cell expression More than 60% ++ Excellent cell expression

Evaluation date division Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 7 days after application Hair root cell expression 15 5 8 15 8 Scoring ± - - ± - Judgment Partial expression No expression No expression Partial expression No expression 14 days after application Hair root cell expression 75 10 15 55 20 Scoring ++ - ± + ± Judgment Excellent expression No expression Partial expression Good expression Partial expression

Inflammatory cell expression evaluation is to compare the degree of expression of inflammatory cells by skin irritation or stress, as shown in Table 6 and Figure 3, in the case of Comparative Example 1 in which severe skin irritation and erythema appeared severely more inflammatory cells Expression could be confirmed.

As a result of the scalp and hair improvement animal test of Examples 1 and Comparative Examples 1 to 4, the most important matter in all hair promoting compositions should be a safe material that does not cause irritation and inflammation, and the primary hair root cell growth and 2 Proper formulation of the primary hair growth promoting material is expected to exhibit greater hair growth promoting effect, and it was confirmed that the mixed herbal medicine extract according to the present invention has the above-described effect.

Evaluation date division Preparation Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 7 days after application Inflammatory cell expression 0 5 2 0 2 Scoring - - - - - Judgment No expression No expression No expression No expression No expression 14 days after application Inflammatory cell expression 8 25 10 8 15 Scoring - ± - - ± Judgment No expression Partial expression No expression No expression Partial expression

Experimental Example 2 Anti-inflammatory Effect Test

In order to measure the anti-inflammatory effect of the scalp and hair improvement herbal extracts presented in the present invention, the lipoxygenase inhibitory effect was measured. 5-lipoxygenase (5-LO) is an enzyme that acts on the first reaction of leukotrienes biosynthesis involved in various inflammatory and allergic diseases. Therefore, the anti-inflammatory effect was confirmed by measuring the inhibitory effect of lipoxygenase using SLO (soybean lipoxygenase) similar to 5-LO structurally and mechanism. The procedure for measuring the inhibitory effect of lipoxygenase is as follows. To 1 ml of 1 mM linoleic acid, herbal extracts were added at concentrations of 0.3, 1.3, and 2.5% (v / v), and 0.9 ml of 50,000 units / ml lipoxygenase dissolved in 0.1 M sodium phosphate buffer was added. It was made to react for 15 minutes on the water bath of ° C. 0.5 ml of 20% (w / v) trichloroacetic acid was added to terminate the reaction, and 1 ml of 0.6% (w / v) TBA was added and developed in boiling water for 15 minutes. After cooling for 2 minutes in iced water, 2 ml of n-butanol was added and shake-mixed, and centrifuged for 5 minutes at 3,500 rpm. The anti-inflammatory effect (%) was evaluated by taking the upper layer (n-butanol layer) and measuring the absorbance at 535 nm. The control group used a solvent instead of the mixed extract. The blank used 0.1 M sodium phosphate buffer instead of lipoxygenase. The lipoxygenase inhibitory effect was calculated by the following Equation 1 and the measurement results are shown in FIG. 4.

As shown in Figure 4, the anti-inflammatory effect of the scalp and hair improvement herbal medicine extract provided in the present invention showed an anti-inflammatory effect of 7.2% at a concentration of 0.3% (v / v) of the herbal medicine extract, 1.3% (v / v) Showed an anti-inflammatory effect of 25.3%. In addition, IC _{50 with} 50% lipoxygenase inhibitory effect In the case of value, the anti-inflammatory effect of the scalp and hair improvement herbal medicine extract provided by the present invention was confirmed by checking the concentration of the herbal extract 2.5% (v / v).

Formulation Example  1 and its Comparative example  One

The composition of the shampoo comprising the scalp and hair improvement herbal medicine extract of the present invention was prepared by configuring as shown in Table 7. At this time, the unit of component content is weight%. Among the components identified by each component number in Table 7 below, component 11 was first heated at a temperature of 70 ° C., and the other components were added to dissolve uniformly. Then, component 12 was added thereto, stirred, and cooled to room temperature. The formulation of Comparative Example 1 for Formulation Example 1, except for the component 12, the composition of the other components or the preparation method was prepared in the same manner.

number Ingredient Name Formulation Example 1 Formulation Comparative Example 1 One Sodium laureth sulfate 30.0 30.0 2 Potassium cocoyl glycinate 5.0 5.0 3 Cocamide Diei 2.0 2.0 4 Cocamidopropyl Betaine 5.0 5.0 5 glycerin 1.0 1.0 6 Spices a very small amount a very small amount 7 Pigment Quantity Quantity 8 antiseptic a very small amount a very small amount 9 Metal Ion Blocker Quantity Quantity 10 pH regulator Quantity Quantity 11 Purified water Remaining amount Remaining amount 12 Scalp and Hair Improvement Herbal Extract 20.0 -

Formulation Example  2-3 and its Comparative example  2

The composition of the hair treatment of the creams including the scalp and hair improvement herbal medicine extract of the present invention was prepared as shown in Table 8. At this time, the unit of component content is weight%. Among the components identified by each component number in Table 8, components 1 to 5 were first dissolved by heating at a temperature of 80 ° C., and then components 6 to 14 were dissolved by heating, adjusted to 80 ° C., and mixed with the components 1 to 5 mixture with stirring. After emulsification with a homomixer. Thereafter, the emulsified product was cooled to a temperature of 30 ° C., and then components 13 and 14 were added and stirred until uniform. The formulation of Comparative Example 2 for Formulation Examples 2 and 3 except for component 14, the composition or preparation method was the same.

number Ingredient Name Formulation Example 2 Formulation Example 3 Formulation Comparative Example 2 One Cetearyl Alcohol 5.0 5.0 5.0 2 Self-emulsifying glyceryl stearate 1.0 1.0 1.0 3 Liquid paraffin 2.0 2.0 2.0 4 antiseptic a very small amount a very small amount a very small amount 5 Spices a very small amount a very small amount a very small amount 6 Purified water Remaining amount Remaining amount Remaining amount 7 Carboxy Vinyl Polymer 0.1 0.1 0.1 8 Hydroxyethyl cellulose 0.2 0.2 0.2 9 glycerin 2.0 2.0 2.0 10 Stearyltrimethylammonium chloride 1.0 1.0 1.0 11 Quaternium-18 2.0 2.0 2.0 12 Stearamidopropyl Dimethylamine 2.0 2.0 2.0 13 Metal Ion Blocker Quantity Quantity Quantity 14 Pigment Quantity Quantity Quantity 15 pH regulator Quantity Quantity Quantity 16 Scalp and Hair Improvement Herbal Extract 10.0 20.0 -

Experimental Example 3 Antimicrobial Effect of the Scalp and Hair Improvement Cosmetic Composition

Antibacterial effect of the scalp and hair improvement cosmetic composition according to the present invention is dandruff ( Malassezia furfur , KCTC 7546) was evaluated using the paper disk method to evaluate the size of the clear zone (clear zone) of the sterilization range around the disk.

Formulation Example 1 and Formulation Comparative Example 1 prepared in Table 7 were used as samples, and purified water was used as a control. After diluting the cultured dandruff by 1/10 to the medium, 0.2 ml is evenly applied to the plate and soaked well, and then 50 ul of water of Formulation Example 1 and Comparative Example 1, and the control water on a 10 mm size paper disk. The disc was planted in the center of the plate and incubated at 35 ° C to observe the condition. The sample was applied to the paper disk and the results were compared.

As shown in FIG. 5, the antimicrobial effect of the scalp and hair improvement cosmetic composition provided by the present invention on the dandruff was not clear zone in the purified water treated sample as a control, and in the case of the sample treated with Comparative Example 1, the clear zone was Hardly formed. On the other hand, the formulation of Example 1, which is a scalp and hair improvement cosmetic composition provided by the present invention, when a sample having a clear zone of about 30 mm appears, the antimicrobial activity of the scalp and hair improvement cosmetic composition provided by the present invention against dandruff. The effect was confirmed to be much superior to the comparative example.

Experimental Example 4 Wool Effect of Scalp and Hair Improvement Cosmetic Composition

The wool effect of the scalp and hair improvement cosmetic composition according to the present invention was evaluated by using the hair tensile strength measurement method, which is one of the methods for measuring the health of hair. The tensile strength of hair is measured by applying force to the hair and calculating the relationship between weight and extension at the unit area of hair.

Formulation Example 2 and Formulation Comparative Example 2 prepared in Table 8 were used, and a sample hair cut at 30 cm of a female hair of about 15 years old was used as a sample hair by Rheometer (Compac-100, Sun Science Co.). Hair tensile strength was measured. 0.4 ml of Formulation Example 2 and Formulation Comparative Example 2 were evenly applied to the sample hair, then massaged for 1 minute, rinsed thoroughly with water, and the hair was dried by cold wind of hair dryer to measure the tensile strength of the hair 10 times per formulation. . Water was used as a control. The process was repeated one cycle (one cycle per day), and a total of three cycles were repeated to measure the change in tensile strength.

As a result of the evaluation of the tensile strength of the hair, as shown in FIG. 6, after 3 cycles in the case of Formulation Example 2, which is the scalp and the hair improvement cosmetic composition provided in the present invention, the tensile strength was confirmed to remain substantially constant. When Formulation Example 2 was applied, it was confirmed that the breaking weight after 1 cycle was 136 g, the breaking weight after 2 cycles was 136 g, and the breaking weight after 3 cycles was maintained at approximately constant 140 g. On the other hand, in Comparative Example 2 and the control it was confirmed that the tensile strength gradually decreased. In the case of the control group coated with water, the tensile strength was decreased to 137 g after 1 cycle, 127 g after 2 cycles, and 123 g after 3 cycles, and 1 cycle when the formulation of Comparative Example 2 was applied. After 137 g, after 2 cycles 123 g, after 3 cycles was confirmed to significantly decrease to 114 g. If the tensile strength is maintained or increased after the hair product treatment, the wool effect of the sample used may be judged to be good. On the other hand, when the tensile strength decreases after treating the sample as in the case of Comparative Example 2 or the control, it can be determined that the damage of the hair is increased. As described above, it was confirmed that the wool effect of the scalp and the hair improvement cosmetic composition provided by the present invention was good through the measurement of the tensile strength of the hair.

Experimental Example 5 Half-head Test of Scalp and Hair Improvement Cosmetic Composition

In order to evaluate the usability of the scalp and hair improvement cosmetic composition containing the scalp and hair improvement herbal medicine extract, the hair treatment cosmetic composition prepared in Examples 2 and 3 of Table 8, and Comparative Example 2 Half A head test (C. Robbins. Chemical and physical behavior of human hair, Von Nostrand Reinhold, New York, 102-110, 1979) was performed.

After applying the hair treatment cosmetic composition of the formulations Examples 2 and 3 and Comparative Example 2 to the hair according to the general usage for 20 women aged 25 to 35 years, foaming, shine, smoothness, touch when using, Satisfaction with touch after use, touch after drying and combing after drying were evaluated. The sensory evaluation was based on the five-point method: very good (A, 5 points), good (B, 3 points), normal (C, 0), bad (D, -3 points), very bad (E, -5 points). Based on the calculation score was calculated by the following equation (2). The evaluation results are shown in Table 9 below.

division Formulation Example 2 Formulation Example 3 Formulation Comparative Example 2 Bubble 3.75 3.85 3.50 burnish 3.30 3.70 2.70 lubricity 3.25 3.60 3.05 Feel when used 3.80 3.80 3.65 Feel after use 3.40 3.45 3.15 Feel after drying 3.30 3.40 3.20 Combing after drying 3.95 4.05 3.65

As shown in Table 9, Formulation Examples 2 and 3, which is a cosmetic composition including the scalp and hair improvement herbal medicine extract, are very satisfied with each evaluation item compared to Comparative Formulation 2, which does not include the herbal medicine extract. This result is because the scalp and hair improvement herbal extracts have an excellent effect on skin soothing effect, wool effect, moisturizing effect and the like.

1 is a photograph of the hair growth test using a C57BL mouse as a result showing the hair growth effect of the scalp and hair composition according to an embodiment of the present invention.

Figure 2 is a photograph of the hair follicle cells taken by optical microscopy after cutting and staining the mouse skin in the hair growth test using a C57BL mouse as a result showing the hair follicle cell expression effect of the scalp and hair composition according to an embodiment of the present invention. .

3 is a photograph of inflammatory cells taken with an optical microscope after cutting and staining mouse skin in a hair growth test using a C57BL mouse as a result showing the inflammatory cell expression effect of the scalp and hair composition according to an embodiment of the present invention. .

Figure 4 is a chart showing the lipoxygenase inhibitory effect according to the concentration of the herbal extracts as a result showing the anti-inflammatory effect of the scalp and hair composition according to an embodiment of the present invention.

Figure 5 is a diagram showing the tensile effect of the hair treated with the cosmetic composition as a diagram showing the effect of the wool composition of the cosmetic composition including the scalp and hair herbal extract according to an embodiment of the present invention.

Figure 6 is a photograph showing the antimicrobial activity against dandruff as a result showing the antimicrobial effect of the cosmetic composition comprising the herbal extract for scalp and hair according to an embodiment of the present invention.

Claims (4)

Angelica, golgoji, white leaf, nasturtium, gojija, bokbunja, sangbaekpi, sukjiwang, yeojeongsil, red sesame, black jima, red ginseng, white paper, motherwort, sweet ginseng, ginseng, myrrh, gamguk, frankincense, ginseng, cheongung, haphwanpi Cosmetic composition for scalp and hair comprising a mixed herbal medicine extract. According to claim 1, wherein the mixed herbal medicines 1 to 5 parts by weight of Angelica, Bogolgol, baekryeop and nasturtium, respectively; 0.5 to 6 parts by weight of Gugija, Bokbunja, Sangbaekpi, Sukjihwang, Journey, Red Sewage, and Black Horse; 1 to 20 parts by weight of red ginseng, white paper and motherwort, respectively; And 0.5 to 4 parts by weight of sweet ginseng, cabbage, myrrh, gamguk, frankincense, ginseng, Cheongung, Haphwanpi and Hyunho colors, respectively. According to claim 1, wherein the extract is scalp and hair cosmetic composition, characterized in that extracted with one or more solvents selected from the group consisting of water, ethanol, glycerin, butylene glycol and propylene glycol. According to claim 1, wherein the composition is a cosmetic composition for scalp and hair, characterized in that any one selected from the group consisting of lotion, lotion, cream and shampoo.

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