CN115006468A

CN115006468A - Compound Chinese herbal medicine hair tonic and preparation method thereof

Info

Publication number: CN115006468A
Application number: CN202210752889.3A
Authority: CN
Inventors: 崔宁; 上官静波; 许宁侠; 吴康
Original assignee: Xian International University
Current assignee: Guangzhou Huaxia Biopharmaceutical Co ltd
Priority date: 2022-06-28
Filing date: 2022-06-28
Publication date: 2022-09-06
Anticipated expiration: 2042-06-28
Also published as: CN115006468B

Abstract

The invention provides a compound Chinese herbal medicine hair tonic, which comprises an A phase, a B phase and a C phase, wherein the total mass percentage is as follows: the phase A comprises: 1, 3-butanediol 12%, glycerol 5%, transparent xanthan gum 0.1% and deionized water to 100%; the phase B comprises: composite Chinese herbal medicine extract 30 percent of panthenol and 0.5 percent of nicotinamide; the phase C comprises: 0.5 percent of phenoxyethanol. The compound Chinese herbal medicine hair restorer disclosed by the invention can promote the hair regeneration speed through verification, so that the grown hair is more smooth and glossy. The invention also provides a preparation method of the compound Chinese herbal medicine hair tonic.

Description

Compound Chinese herbal medicine hair tonic and preparation method thereof

Technical Field

The invention relates to a compound Chinese medicinal preparation, in particular to a compound Chinese herbal medicine hair tonic.

Background

According to statistics, the number of existing alopecia patients in China reaches 1.68 hundred million, wherein androgenetic alopecia and alopecia areata are taken as main alopecia types, and account for more than 90 percent of alopecia. Alopecia affects the appearance and brings stress and trouble to patients, and the patients with alopecia are also easy to have nail defects and have complications such as eye diseases, leucoderma, hypothyroidism and the like. The number of patients with alopecia is increased year by year and the patients tend to be younger, the market demand of hair growth products is increased day by day, and the market value can reach about 100 hundred million. In the treatment of alopecia, currently, the western medicines commonly used in clinic are finasteride and minoxidil. However, the two medicines have low cure rate and are easy to relapse after stopping taking the medicines, and side effects with different degrees, such as scalp pruritus, dry hair, dizziness, cold sweat, anorexia, myocardial infarction and the like, can also appear after long-term use. Therefore, the development of safer and more efficient drugs for treating alopecia has become a hot research point.

The invention aims to provide a compound Chinese herbal medicine hair tonic.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention provides a compound Chinese herbal medicine hair growth promoting liquid which can effectively promote hair growth and improve alopecia.

In order to achieve the purpose, the compound Chinese herbal medicine hair tonic of the invention comprises an A phase, a B phase and a C phase, wherein the total mass percentage is as follows:

the phase A comprises:

1, 3-butanediol 12%

Glycerol 5%

0.1 percent of transparent xanthan gum

Deionized water to 100%;

the phase B comprises:

30 percent of compound Chinese herbal medicine extract

Panthenol 2%

0.5 percent of nicotinamide;

the phase C comprises:

0.5 percent of phenoxyethanol.

Further, the compound Chinese herbal medicine extract is extracted from mixed Chinese medicinal materials, and the mixed Chinese medicinal materials comprise the following components in parts by weight:

prepared fleece flower root 6 parts

6 portions of salvia miltiorrhiza

6 portions of Szechuan lovage rhizome

Cacumen biotae 3 parts

3 portions of liquorice

3 parts of black sesame

Prepared rehmannia root 6 parts

6 parts of glossy privet fruit.

Further, the compound Chinese herbal medicine extract is obtained by extracting the mixed Chinese herbal medicines by ethanol.

Further, the compound Chinese herbal medicine extracting solution also comprises:

melittin 200ng/ml

Sodium thiosulfate 4% (w/w).

The invention also provides a preparation method of the compound Chinese herbal medicine hair tonic, which comprises the following steps:

s1: weighing a specified amount of traditional Chinese medicinal materials including radix polygoni multiflori preparata, salvia miltiorrhiza, ligusticum chuanxiong hort, cacumen biotae, liquorice, black sesame, prepared rehmannia root and glossy privet fruit respectively, mixing, cleaning for 2-3 times by using clear water, and airing for later use;

s2: adding 75% ethanol into the above mixed Chinese medicinal materials, ultrasonic extracting for 30min, filtering, collecting filtrate, and repeating once;

s3: mixing the two filtrates, concentrating under reduced pressure, mixing, centrifuging, and filtering to obtain compound Chinese medicinal extractive solution;

s4: accurately weighing each substance in phase A, placing in a beaker with proper size, heating in a constant temperature water bath kettle at 85 deg.C for 30min, taking out, stirring with a glass rod, cooling to 40 deg.C, sequentially adding phase B and phase C, and mixing completely;

s5: adding melittin and sodium thiosulfate, and mixing to obtain the final product.

Radix Polygoni Multiflori Preparata and fructus Ligustri Lucidi have effects of nourishing yin, invigorating kidney, promoting hair follicle development and hair growth without leaving blood supply of capillary vessel of hair papilla. The active ingredients of the cacumen biotae mainly comprise essential oil and yellow wine, and the active ingredients of the cacumen biotae have obvious anti-inflammatory effect and have inhibition effect on staphylococcus aureus, escherichia coli and tetragenococcus. Tannin in folium Platycladi has antioxidant, free radical scavenging and antitumor activities. The arborvitae tops alcohol extract and the volatile oil thereof can delay hair loss to a certain extent. Fructus Ligustri Lucidi is fruit of Ligustrum lucidum belonging to Ligustrum of Oleaceae, has cool, dry and slightly bitter taste, and has effects of improving eyesight, blackening hair, and nourishing liver and kidney, and can be used for treating diseases such as liver and kidney yin deficiency, giddiness tinnitus, soreness of waist and knees, and early white beard and hair. The fructus Ligustri Lucidi alcohol extract has effect in promoting hair growth for alopecia caused by androgen. The prepared rehmannia root has the effects of tonifying kidney, promoting hair growth, nourishing yin, enriching blood, promoting blood circulation to remove blood stasis, clearing heat, promoting diuresis, dispelling wind and removing dampness.

The compound Chinese herbal medicine hair restorer has the effect of promoting hair growth, can improve the hair regeneration speed and enables the hair to be more smooth and glossy.

Drawings

The present invention will be further described and illustrated with reference to the following drawings.

Fig. 1 is a graph of staged hair growth in mice.

FIG. 2 is a graph of mouse hair growth scores

FIG. 3 is a section of mouse hair growth and hair follicle tissue under light microscope.

Detailed Description

The technical solution of the present invention will be more clearly and completely explained by the description of the preferred embodiments of the present invention with reference to the accompanying drawings.

Example 1: the preparation of the compound Chinese herbal medicine hair tonic comprises the following steps:

s1: weighing prescribed amount of traditional Chinese medicinal materials including 6g of radix polygoni multiflori preparata, 6g of salvia miltiorrhiza, 6g of ligusticum chuanxiong hort, 3g of cacumen biotae, 3g of liquorice, 3g of black sesame, 6g of prepared rehmannia root and 6g of glossy privet fruit respectively, mixing, cleaning with clear water for 2-3 times, and airing for later use;

s2: adding 400ml of 75% ethanol into the mixed Chinese medicinal materials, performing ultrasonic extraction for 30min, filtering, collecting filtrate, and repeating the above steps once;

s3: mixing the two filtrates, concentrating under reduced pressure to 470ml, mixing, centrifuging, and filtering to obtain compound Chinese herbal medicine extractive solution;

s4: accurately weigh phases A, B and C, each matching the ratio as in Table 1 below

TABLE 1 compatibility of compound Chinese herbal medicine hair restorer

Putting the substances in the phase A into a beaker with a proper size, putting the beaker in a constant-temperature water bath kettle at 85 ℃, heating the beaker for 30min, taking the heated beaker out, stirring the heated beaker by a glass rod, cooling the heated beaker to 40 ℃, sequentially adding the phase B and the phase C, and fully mixing the phases;

s5: adding melittin to a concentration of 200ng/ml and sodium thiosulfate to 4% (w/w), and mixing to obtain the final product.

Example 2: the difference between the preparation of the compound Chinese herbal medicine hair tonic and the example 1 is that in the step S3, the two filtrates are combined and concentrated to 235ml under reduced pressure.

Example 3: the difference between the preparation of the compound Chinese herbal medicine hair tonic and the example 1 is that in the step S3, the two filtrates are combined and concentrated to 160ml under reduced pressure.

The effect of the compound Chinese herbal medicine hair tonic is verified:

1. establishing an alopecia animal model:

30 male C57BL/6 mice were acclimatized for 1 week, then divided into 5 groups (blank group, MNX 2%, example 1, example 2 and example 3) by weight and using Excel software, and the initial color of each back of each group was photographed and recorded in detail, and depilatory cream was used to depilate the back of each group about 3X 4cm ² The skin was pink and unbroken, confirming that the hair follicles in the mice were in resting stage. The feed is fed regularly and quantitatively every day, and each mouse can drink water freely by 6g of common feed per day on average.

2. Use of group mouse drugs

Minoxidil is currently used as an externally applied medicine for treating alopecia in clinic, and is used as a positive control of an externally applied hair growth promoting effect experiment in the experiment. Specific administration methods are shown in table 2:

table 2 use of group mouse drugs

3. Observation of Hair growth

The skin and hair growth conditions of the hair removal area of each group of mice were observed every day, and the time when the skin color of the hair removal area of each mouse changed from pink to black and the time when hair growth began were recorded, and the effect of the drug on the hair growth cycle of the mice was evaluated. At the same time, the mice in each group were photographed at

day

1, 14, 21 and 28, and the mice were subjected to the periodic hair growth as shown in fig. 1.

As can be seen from fig. 1, after 1d, there was no significant change in the mice of each group, and the skin remained pink; after 14d of the experiment, the skin color of the shaved area on the back of the mouse becomes black, wherein in the treatment group of the example 3, the skin color of the mouse is relatively uniformly restored, and black villi grow in a small area; after 21d of the experiment, the basic skin of each group of mice completely turns black, but the hair growth of the compound Chinese herbal medicine hair-restorer treatment group is more uniform than that of the blank group and the minol treatment group; after 28 days of the experiment, the hair of each group of mice is visible, wherein the hair length of individual mice in the treatment group of the example 3 has no obvious difference from other parts, and the hair of the mice in the treatment group of the example 2 grows more uniformly. In appearance, the new hair of the mice treated by the compound Chinese herbal medicine hair restorer is darker and brighter than the hair of other parts.

Statistics for the observation of hair growth status in mice are shown in table 3.

TABLE 3 time table for skin discoloration and hair growth

The hair growth of each group of mice was scored on

days

1, 7, 14, 21, and 28. Grading standard: 0 point (the skin color of the depilatory area is pink and no hair grows); score 1 (skin color gray and hair growth less than 20% in the depilated area); 2 points (the skin color of the depilated area is gray black and the hair growth degree is more than 20 percent and less than 40 percent); 3 points (the skin color of the depilated area is light black and the hair growth degree is more than 40 percent and less than 60 percent); 4, dividing (the skin color of the depilatory area is black and the hair growth degree is more than 60 percent and less than 80 percent); 5 points (the hair in the depilating area is basically full and the hair growth degree is more than 80 percent).

As shown in fig. 2, the mouse score was linear with time. It is clear that the difference in scores among the groups of mice is not very large over the first 7 days of growth, whereas the scores among the groups of mice are clearly divided from day 14 onwards.

The score of example 2 was significantly higher than the other groups, while the blank group was also significantly lower than the other groups starting at day 14.

4. Determination of mouse hair length and hair weight and calculation of hair density

The compound Chinese herbal medicine hair-restorer and minoxidil are topically applied to dorsal skin of mice 28 d. After the experiment, the mice were sacrificed by cervical dislocation, and the skin of the mouse depilation region was cut off at the same position of each group of mice by 3X 4cm ² Scraping off the hair on the skin patch by using a scalpel, weighing the skin patch on an analytical balance, and calculating the average value of the hair weight of the new hair of the mouse; measuring the length of the hair by a digital display caliper and calculating a mean value; and the wool density per unit area was calculated as wool weight/wool length. And meanwhile, analyzing by using a statistical method, and checking to judge whether significant differences exist, wherein the data are expressed by mean standard deviation (S.D.).

The results of the collection and measurement of the hair weight and the hair length of the obtained mice are shown in Table 4.

Table 4 the hair length and hair weight of each group of mice (n-6,

)

it can be seen that the hair growth of mice smeared with the compound Chinese herbal hair-restorer and minoxidil was significantly increased compared to the blank group. Among them, the data of the hair length, the hair weight and the hair density of the mice in the treatment group of example 2 are better.

5. Histological observation

The compound Chinese herbal medicine hair-restorer is smeared on the back of a mouse for 28 days by using a method of a table 2, the mouse is killed by a cervical vertebra dislocation method after the experiment is finished, long strip-shaped unhaired skin with the width of 1cm is cut parallel to the spine of the mouse and is lightly pasted on a hard board, wrinkles and clamp marks of tweezers are avoided, and at the moment, the paraformaldehyde fixing liquid is rapidly used for fixing for 48 hours. Then, washing with water, trimming, dehydrating, waxing, embedding, and slicing along the direction of hair growth (6 μm); then, the prepared roast slices were subjected to hematoxylin and eosin staining and mounting. Finally, histological changes of the hair follicles of the skin of the mice were observed under an optical microscope, recorded by photography, counted and measured in diameter. The hair growth and the histological appearance of the follicles of the mice under the light microscope are shown in fig. 3.

As can be seen from FIG. 3, the histological appearance of all the mice in the drug group is obviously different from that of the mice in the blank group, and the hair follicles of the mice using the compound Chinese herbal medicine extract and minoxidil are obviously enlarged and increased, so that the melanin in hair bulbs is also accumulated more. The blank group had thinner skin and the lowest thickness of subcutaneous tissue, almost all hair follicles grew in the dermis, and the color in the hair bulb was lighter, indicating that the hair of the mice in the blank group was still in the resting stage and had less melanin accumulation. The hair of the hair restorer group of compound Chinese herbal medicine has thicker fat cell layer around the hair, especially the fat cell layer of the embodiment 2 is thickest, the size of the vacuolated fat cells is uniform, the shape is clear and visible, the cells are arranged compactly and tidily along the hair growth direction, the fat cell layer extends to the dermis layer, the hair of the group grows uniformly and ideally, and meanwhile, the epidermis layer, the dermis layer and the muscle layer are arranged neatly and tightly.

In conclusion, the compound Chinese herbal medicine hair growth promoting liquid has the effect of promoting hair growth, can improve the hair regeneration speed, and enables the hair to be more smooth and glossy.

The above detailed description merely describes preferred embodiments of the present invention and does not limit the scope of the invention. Without departing from the spirit and scope of the present invention, it should be understood that various changes, substitutions and alterations can be made herein by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims and their equivalents. The scope of the invention is defined by the claims.

Claims

1. The compound Chinese herbal medicine hair tonic is characterized by comprising an A phase, a B phase and a C phase, wherein the total mass percentage is as follows:

the phase A comprises:

1, 3-butanediol 12%

Glycerol 5%

0.1 percent of transparent xanthan gum

Deionized water to 100%;

the phase B comprises:

30 percent of compound Chinese herbal medicine extract

Panthenol 2%

0.5 percent of nicotinamide;

the phase C comprises:

0.5 percent of phenoxyethanol.

2. The compound Chinese herbal medicine hair tonic according to claim 1, wherein the compound Chinese herbal medicine extracting solution is extracted from mixed Chinese herbal medicines, and the mixed Chinese herbal medicines comprise the following components in parts by weight:

prepared fleece flower root 6 parts

6 portions of salvia miltiorrhiza

6 portions of Szechuan lovage rhizome

Cacumen biotae 3 parts

3 portions of liquorice

3 parts of black sesame

Prepared rehmannia root 6 parts

6 parts of glossy privet fruit.

3. The compound Chinese herbal medicine hair tonic according to claim 2, wherein the compound Chinese herbal medicine extract is obtained by extracting mixed Chinese herbal medicines with ethanol.

4. The compound Chinese herbal medicine hair tonic according to claim 2, characterized in that the compound Chinese herbal medicine extract further comprises:

melittin 200ng/ml

Sodium thiosulfate 4% (w/w).

5. The preparation method of the compound Chinese herbal medicine hair tonic according to any one of claims 1 to 4, characterized by comprising the following steps: