CN108066274B

CN108066274B - Hair blackening compound and preparation method and application thereof

Info

Publication number: CN108066274B
Application number: CN201810074583.0A
Authority: CN
Inventors: 张晓松; 许洋
Original assignee: Shenzhen Giant Crocodile Biotechnology Co ltd
Current assignee: Shenzhen Huayuan Biotechnology Co ltd
Priority date: 2018-01-25
Filing date: 2018-01-25
Publication date: 2021-03-30
Anticipated expiration: 2038-01-25
Also published as: CN108066274A

Abstract

The invention relates to a hair blackening compound and a preparation method and application thereof. The hair blackening compound comprises the following raw materials in parts by weight: 3 to 12 parts of crocodile oil, 2 to 8 parts of crocodile gall, 18 to 25 parts of fleece-flower root, 18 to 23 parts of black bean, 8 to 20 parts of black sesame, 5 to 15 parts of safflower, 6 to 18 parts of desertliving cistanche, 18 to 25 parts of purslane, 10 to 23 parts of dodder and 4 to 12 parts of glossy privet fruit. The hair blackening compound has the effects of relieving exterior syndrome, clearing heat, nourishing yin, removing yang, cooling blood, generating blood, tonifying deficiency and blackening hair, and has long action time and good hair blackening effect.

Description

Hair blackening compound and preparation method and application thereof

Technical Field

The invention relates to a hair blackening compound and a preparation method and application thereof.

Background

Under the background that the pace of modern life is increasingly accelerated, white hair is very common in daily life and often brings very heavy psychological stress to patients. White hair is mainly caused by reduced melanin formation in hair, reduced melanin formation by melanocytes, and reduced tyrosinase activity. The existing common hair blackening products adopt bleaching and dyeing to blacken hair, newly generated hair is still white along with the continuous metabolism of a human body, the problem of blackening the hair is not fundamentally solved, the temporary solution and the permanent solution are treated, and the existing partial hair dyes have great harm to the scalp and even contain carcinogenic substances and possibly cause lesion. At present, the hair blackening product with the traditional Chinese medicine components on the market has a certain effect, but the effect degree is not obvious, and most of white hair people have more hair dyeing.

Disclosure of Invention

Therefore, it is necessary to provide a hair blackening compound and a preparation method and application thereof aiming at the problem of poor effect of the traditional Chinese medicine hair blackening product.

The hair blackening compound comprises the following raw materials in parts by mass:

3 to 12 parts of crocodile oil, 2 to 8 parts of crocodile gall, 18 to 25 parts of fleece-flower root, 18 to 23 parts of black bean, 8 to 20 parts of black sesame, 5 to 15 parts of safflower, 6 to 18 parts of desertliving cistanche, 18 to 25 parts of purslane, 10 to 23 parts of dodder and 4 to 12 parts of glossy privet fruit.

The hair blackening compound does not contain artificial synthetic substances, comprises rich amino acids, polyunsaturated fatty acids, various mineral substances and trace elements, can increase the oxygen carrying function of scalp capillaries, activates hair follicles, enables the hair follicles to acquire the capability of continuously secreting tyrosinase, promotes the generation of hair follicle cells tyrosinase, and simultaneously promotes the hair papilla to secrete melanin. The hair blackening composition has the effects of relieving exterior syndrome, clearing heat, nourishing yin, removing yang, cooling blood, promoting hematopoiesis, tonifying deficiency and blackening hair; the hair blackening compound has the advantages of long effective action time, no toxic or side effect, wide application range and good hair blackening effect through experimental determination.

In one embodiment, the raw materials of the hair blackening compound comprise:

4 to 12 parts of crocodile oil, 3 to 8 parts of crocodile gall, 20 to 25 parts of fleece-flower root, 19 to 23 parts of black bean, 10 to 20 parts of black sesame, 7 to 15 parts of safflower, 8 to 18 parts of desertliving cistanche, 19 to 25 parts of purslane, 12 to 23 parts of dodder and 6 to 12 parts of glossy privet fruit.

In one embodiment, the raw materials of the hair blackening compound comprise:

6 to 12 parts of crocodile oil, 4 to 8 parts of crocodile gall, 21 to 25 parts of fleece-flower root, 20 to 23 parts of black bean, 12 to 20 parts of black sesame, 9 to 15 parts of safflower, 10 to 18 parts of desertliving cistanche, 20 to 25 parts of purslane, 15 to 23 parts of dodder and 8 to 10 parts of glossy privet fruit.

In one embodiment, the raw materials of the hair blackening compound comprise:

10 parts of crocodile oil, 7 parts of crocodile gall, 24 parts of polygonum multiflorum, 22 parts of black beans, 18 parts of black sesame, 14 parts of safflower, 16 parts of cistanche, 24 parts of purslane, 22 parts of semen cuscutae and 9 parts of glossy privet fruit.

A hair blackening composition comprises plant extract, crocodile oil and crocodile gall extract; the plant extract comprises, by mass, 18-25 parts of polygonum multiflorum, 18-23 parts of black beans, 8-20 parts of black sesame, 5-15 parts of safflower, 6-18 parts of cistanche, 18-25 parts of purslane, 10-23 parts of semen cuscutae, 4-12 parts of glossy privet fruits, 3-12 parts of alligator oil and 2-8 parts of alligator gall.

The preparation method of the hair blackening compound is characterized by comprising the following steps of:

mixing plant extracts obtained from the raw materials of the polygonum multiflorum, the black beans, the black sesame, the safflower, the cistanche, the purslane and the semen cuscutae, crocodile gall extracts obtained from the raw materials of crocodile gall and crocodile oil to obtain a hair blackening compound.

In one embodiment, the plant extract is prepared by the steps of: performing supercritical carbon dioxide extraction on the polygonum multiflorum, the black beans, the black sesame, the safflower, the cistanche, the purslane and the semen cuscutae to obtain plant extracts.

In one embodiment, the method comprises the following steps: and mixing the plant extract, the crocodile oil and the crocodile gall extract to obtain the hair blackening compound.

The application of the hair blackening compound in washing and caring products or preparing hair blackening medicines.

A hair cream, which is characterized by comprising the hair blackening compound.

Detailed Description

In order to facilitate an understanding of the present invention, the following description will be made more fully with reference primarily to the black hair compound and its method of preparation and use. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

The crocodile oil contains rich amino acids, polyunsaturated fatty acids (such as DHA, DPA and EPA), trace elements and the like, can stimulate human cells to secrete cell recombination factors, decompose aging damaged cells, promote metabolism and enhance cell viability, and meanwhile, has good permeability and is very easy to accept and absorb by the human body. The crocodile oil used as the raw material of the hair blackening compound has the effects of supplementing nutrient substances for hair follicles, increasing the oxygen carrying function of scalp capillaries, activating hair follicles and promoting the secretion of melanin from hair papillae. The alligator oil is from Siamese alligator, is yellow oily liquid, insoluble in water and inflammable, and is purchased from Guangdong Hongyi alligator industry Co.

The crocodile bile has the functions of clearing away heat, resisting inflammation, promoting granulation, promoting circulation and resisting oxidation. The crocodile bile is used as a raw material of the black hair compound, is beneficial to the metabolism of scalp cells, and has different degrees of inhibition effects on the growth of 10 cancer cells such as cervical cancer cells, osteogenic sarcoma cells, liver cancer cells, bile duct cancer cells, esophageal cancer, breast cancer, lung cancer cells, stomach cancer cells and the like. The crocodile gall of the embodiment is from Siamese crocodile, and the crocodile gall purchased in Thailand is Laura Meida group company.

Polygonum multiflorum, also known as Dijing, hong Nei Xiao, Polygonum multiflorum, Heyue and Ferro-stramonium, is the tuber of Polygonum multiflorum of Polygonum of Polygonaceae. The record of fleece-flower root in compendium of materia medica states that the material is bitter and astringent in taste, can nourish blood, benefit liver, secure essence, tonify kidney, strengthen bones and muscles, blacken hair on temples, and is a good nourishing medicine. The processed product of radix polygoni multiflori has the functions of nourishing liver and kidney, benefiting essence and blood, blackening beard and hair and strengthening bones and muscles. It can be used for treating blood deficiency, sallow complexion, giddiness, tinnitus, premature gray hair, soreness of waist and knees, metrorrhagia, metrostaxis, and leukorrhagia. The main chemical components of the polygonum multiflorum are stilbene, cymbidium ketone and phospholipid. Modern pharmacological research of polygonum multiflorum mainly focuses on the effects of protecting brain, resisting aging, reducing blood fat and protecting liver. As a raw material of the hair blackening compound, the polygonum multiflorum can mildly permeate into scalp, deeply nourish and beautify hair, and promote the continuous regeneration of melanin in hair mother cells. The polygonum multiflorum of the embodiment is prepared polygonum multiflorum, the producing area is Guangdong, and is purchased from Yuzhou area of Yulin city and is recorded as Chinese medicinal material Pujian.

The black beans are sweet in taste and neutral in nature, contain rich vitamins, wherein the vitamins of the E group and the B group are high in content, and the black beans have the effects of relieving exterior syndrome, clearing heat, nourishing blood, calming the liver, tonifying kidney, strengthening yin, tonifying deficiency and blackening hair. As a raw material of the black hair compound, the black soybean seed coat is black, contains anthocyanin, is a good antioxidant, can eliminate free radicals in vivo, and can preserve youthful looks, improve eyesight, blacken hair and the like as recorded in ancient pharmacopoeia. The black beans of the embodiment are produced in northeast and purchased from Shenzhen Shentian district Yi Jia Tong He.

The semen Sesami Niger is black seed of semen Sesami Niger of Pedaliaceae. The efficacy of black sesame in compendium of materia medica is described as tonifying liver, benefiting stomach, nourishing blood, moistening intestines, promoting fluid production, improving eyesight, blackening hair and reducing blood pressure, and is suitable for early white hair, anemia and chlorosis. In recent years, researches show that the black sesame has strong effects on the activation of tyrosinase and the generation promotion of melanin. The black sesame aqueous extract can promote the generation of melanin in B16 cells, and the generation amount of the black sesame aqueous extract is in positive correlation with the activity of tyrosinase; in addition, the gene expression and protein synthesis level of a transcription factor (MITF) related to microphthalmia and tyrosinase of normal human melanocytes can be improved, so that the activity of the tyrosinase can be activated, and the increase of the production amount of melanin can be promoted. As a raw material of the hair blackening compound, the black sesame can increase melanin in vivo and is beneficial to the growth of black hair. The black sesame of the present embodiment is produced in Jiangxi, and purchased from Shenzhen, Fu Tian district, Yi Jia Tong He.

The safflower is pungent in taste and warm in nature, has the effects of activating blood and stimulating menstrual flow, removing blood stasis and relieving pain, and has the effects of activating blood and stimulating menstrual flow, expanding blood vessels, improving microcirculation, eliminating free radicals, resisting inflammation and the like as a raw material of the hair blackening compound. The safflower of the embodiment is purchased from common people pharmacies.

The cistanche deserticola is sweet in taste, salty and warm in nature, has the effects of tonifying kidney yang, benefiting essence and blood and moistening intestinal tracts, and can promote metabolism and activate hair follicles as a raw material of the hair-blackening compound. Cistanche deserticola of the present embodiment is purchased from Yulin Yuzhou district and Chinese medicinal herbs are purchased as parts.

Purslane, also called Wuxing Cao, is recorded in Ben Cao Tu Jing, and is also characterized by its green leaves, red stalks, yellow flowers, white roots and black seeds. The purslane has more components, mainly contains triterpenes, flavonoids, various organic acid potassium salts, calcium salts and other components, has more pharmacological actions, and has the effects of clearing heat and removing toxicity, cooling blood and stopping bleeding. The purslane of the embodiment is purchased from Yulin Yuzhou district of Yulin province Hubei and is recorded as the Chinese medicinal material purchase part.

Semen Cuscutae, also known as HUANGSI, HUATENGZI, and DONGSHENG, is seed of Cuscuta chinensis of Convolvulaceae, and has effects of nourishing liver and kidney, stopping nocturnal emission, reducing urination, preventing miscarriage, improving eyesight, and relieving diarrhea. Can be used for treating spermatorrhea, dribbling urination, enuresis, frequent micturition, soreness of waist and knees, blurred vision, tinnitus, fetal leakage due to kidney deficiency, threatened abortion, and diarrhea due to spleen and kidney deficiency, and for treating paralysis due to wind-heat. The inner Mongolia of the cuscuta chinensis producing area of the embodiment is purchased from Yulin Yuzhou district, Yulin City and records of Chinese herbal medicine Puzhou.

The fructus Ligustri Lucidi is mature fruit of Ligustrum lucidum belonging to family Meliaceae. It is recorded in Shen nong Ben Cao Jing (Shen nong's herbal Jing), has the effects of tonifying liver and kidney, clearing heat and improving eyesight, and can be used for treating deficiency of liver-yin and kidney-yin, dizziness, tinnitus, fatigue and weakness of waist and knees, early white beard and hair, etc. The glossy privet fruit producing area Guangxi of the embodiment is purchased from Yulin Yuzhou district, Yulin City, and is recorded with Chinese medicinal material purchasing parts.

Furthermore, the raw materials of the hair blackening compound comprise 4 to 12 parts of crocodile oil, 3 to 8 parts of crocodile gall, 20 to 25 parts of fleece-flower root, 19 to 23 parts of black bean, 10 to 20 parts of black sesame, 7 to 15 parts of safflower, 8 to 18 parts of desertliving cistanche, 19 to 25 parts of purslane, 12 to 23 parts of dodder and 6 to 12 parts of glossy privet fruit.

Further, the raw materials of the hair blackening compound comprise 6 to 12 parts of crocodile oil, 4 to 8 parts of crocodile gall, 21 to 25 parts of fleece-flower root, 20 to 23 parts of black bean, 12 to 20 parts of black sesame, 9 to 15 parts of safflower, 10 to 18 parts of desertliving cistanche, 20 to 25 parts of purslane, 15 to 23 parts of dodder and 8 to 10 parts of glossy privet fruit

Furthermore, the raw materials of the hair blackening compound comprise 10 parts of crocodile oil, 7 parts of crocodile gall, 24 parts of polygonum multiflorum, 22 parts of black beans, 18 parts of black sesame, 14 parts of safflower, 16 parts of cistanche, 24 parts of purslane, 22 parts of semen cuscutae and 9 parts of glossy privet fruit.

The hair blackening compound does not contain artificial synthetic substances, comprises rich amino acids, polyunsaturated fatty acids, various mineral substances and trace elements, can increase the oxygen carrying function of scalp capillaries, activates hair follicles, enables the hair follicles to acquire the capability of continuously secreting tyrosinase, promotes the generation of hair follicle cells tyrosinase, and simultaneously promotes the hair papilla to secrete melanin. The hair blackening composition has effects of relieving exterior syndrome, clearing heat, nourishing yin, removing yang, cooling blood, promoting hematopoiesis, tonifying deficiency and blackening hair. The experiment tests show that said hair-blackening composition has long effective action time, no toxic side effect, extensive application range and good hair-blackening effect.

Another embodiment of a hair blackening composition comprises a plant extract, alligator oil, and an extract of alligator gall. The plant extract comprises, by mass, 18-25 parts of polygonum multiflorum, 18-23 parts of black beans, 8-20 parts of black sesame, 5-15 parts of safflower, 6-18 parts of cistanche, 18-25 parts of purslane, 10-23 parts of semen cuscutae and 4-12 parts of glossy privet fruits. 3-12 parts of crocodile oil and 2-8 parts of crocodile gall.

The preparation method of the hair blackening compound comprises the following steps:

s110, preparing a plant extract by taking polygonum multiflorum, black beans, black sesame, safflower, cistanche, purslane, glossy privet fruit and semen cuscutae as raw materials, and extracting the crocodile gall extract by taking crocodile gall as a raw material.

In one embodiment, the crocodile feed comprises, by mass, 3-12 parts of crocodile oil, 2-8 parts of crocodile gall, 18-25 parts of polygonum multiflorum, 18-23 parts of black beans, 8-20 parts of black sesame, 5-15 parts of safflower, 6-18 parts of cistanche, 18-25 parts of purslane, 10-23 parts of semen cuscutae and 4-12 parts of glossy privet fruit.

In the above raw materials, the parts by weight of black beans, black sesame, safflower, cistanche, purslane and glossy privet fruit refer to the parts by weight of a dry product, but in some embodiments, fresh raw materials (i.e., raw materials with a high water content and not sufficiently dried) may be used as long as the parts by weight after drying are the above-listed parts.

In one embodiment, the black bean, the black sesame, the safflower, the cistanche and the purslane are boiled with water to obtain a crude extract, and the crude extract is extracted by supercritical carbon dioxide to obtain an extract.

Specifically, the water boiling operation comprises the following steps: mixing black beans, black sesame, safflower, cistanche, purslane and 100-120 parts of water, heating to 100-120 ℃, boiling for 1-2 hours, and filtering to obtain a crude extract.

The supercritical carbon dioxide extraction has low extraction temperature, can effectively prevent the oxidation and the dissipation of heat-sensitive components, and completely retains the biological activity. The whole process of supercritical carbon dioxide extraction does not use organic solvent, has no residual organic solvent, and has the advantages of short extraction time and short production period, thereby being beneficial to ensuring and improving the product quality. Of course, the extract may be prepared by other extraction methods used in the art.

Specifically, the temperature of supercritical carbon dioxide extraction is 31-40 ℃, and the pressure is 18-33 Mpa.

The polygonum multiflorum, the dodder and the glossy privet fruit are crushed into fine powder of 200 meshes, 42 to 60 parts of 95 percent alcohol is added for processing for 24 hours, and the supernatant and the extract are mixed to obtain the plant extract.

In one embodiment, the crocodile gallbladder (dry product) is cut into small pieces (about 5 x 0.5cm), put into 95% alcohol, kept stand and extracted for 12 to 24 hours with the 95% alcohol according to the mass ratio of 1:25 to 1:30 until the crocodile gallbladder becomes a soft thin skin, and then the thin skin residue is discarded to obtain the crocodile gallbladder extraction stock solution. Diluting the crocodile gall extract stock solution by 10-12 times with distilled water to obtain the crocodile gall extract. It can be understood that the crocodile gall stoste can be directly used for preparing the black hair compound as long as the crocodile gall extract in the prepared black hair compound is equivalent to the part of the crocodile gall extract which is extracted and diluted by taking 2-8 parts of crocodile gall as raw materials. Of course, preservative can also be added into the crocodile gall extracting solution to prevent the crocodile gall extracting solution from being changed in quality.

It is understood that the plant extract and the crocodile gall extract are commercially available, that is, the step of S110 may be omitted. The content of the effective components in the purchased plant extract can be equivalent to that of the effective components in the plant extract prepared in the step, and the content of the effective components in the purchased crocodile gall extract can be equivalent to that of the crocodile gall extract prepared in the step.

It is to be understood that the plant extract is prepared by mixing the respective raw materials in the present embodiment, and in other embodiments, the extracts may be combined by extracting the respective raw materials individually.

And S120, mixing the plant extract, the crocodile gall extract and the crocodile oil to obtain a hair blackening compound.

In one embodiment, the hair blackening composition is prepared by mixing the first plant extract and the crocodile gall extract, and then adding crocodile oil.

Specifically, the plant extract and the crocodile gall extract are stirred and mixed for 30-45 min.

Further, adding crocodile oil, mixing, and vacuum homogenizing and emulsifying at 70-80 ℃ to obtain the black hair compound. Specifically, vacuum homogenizing and emulsifying for 45-60 min.

Further, after vacuum homogenization and emulsification, sterilization and subpackage are carried out to obtain the hair blackening compound.

The preparation method of the hair blackening compound can effectively ensure the biological activity of active substances in the raw materials, and has the advantages of environment-friendly preparation process, short production period and high production efficiency.

The daily use method of the black hair compound comprises applying the black hair compound to the roots of the white hair, so that the roots of the white hair are covered by the black hair compound; gently massaging with fingers for 10-25 min, or combing hair along head channels and collaterals and acupoints with a wooden comb (15-20 min), and then staying for 15-30 min to clean hair. The hair blackening composition is used at least three times per week.

The professional use method of the black hair compound comprises the steps of smearing 3 g-10 g of the black hair compound on the roots of white hair, and enabling the roots of the white hair to be covered by the black hair compound; after the introduction operation is carried out for 10 to 20 minutes by using an introduction instrument, steaming is carried out for 10 to 30 minutes by steam; massaging for 5-20 min, and cleaning hair after 1-3 hr.

The hair blackening composition can promote the generation of tyrosinase of hair follicle cells, promote the secretion of melanin of hair papilla, and promote the generation and growth of black hair.

A hair cream comprises the hair blackening compound.

Of course, other acceptable adjuvants for the care product may also be included in the hair cream.

DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION

The following detailed description is given with reference to specific examples.

The following examples, unless otherwise specified, do not include other components except for inevitable impurities.

In the following examples, Polygoni Multiflori radix, semen Sojae Atricolor, semen Sesami Niger, Carthami flos, Cistanchis herba, herba Portulacae, semen Cuscutae, Alligator oil, fructus Ligustri Lucidi and crocodile gallbladder are all cut dry products. Radix polygoni multiflori, herba cistanches, purslane, semen cuscutae and glossy privet fruit are purchased from Yulin Yuzhou district, and Chinese medicinal materials are purchased. Black bean and black sesame are purchased from Shantian district-Yi-Jia-Tonic business in Shenzhen city, safflower is purchased from pharmacy of China, crocodile oil is purchased from Guangdong Hongyi crocodile industry Limited company, and crocodile gall is purchased from Thailand is Lara Meida group company.

The preparation parameters of the embodiments 1 to 3 are shown in the table 1, and the specific steps are as follows:

1) weighing crocodile oil, crocodile gall, polygonum multiflorum, black beans, black sesame, safflower, cistanche, purslane, semen cuscutae and glossy privet fruit, wherein the specific parts by mass are shown in table 2.

2) Mixing black bean, black sesame, safflower, cistanche and purslane with 100 parts of water, boiling for 1 hours at T1 ℃, and filtering to obtain a crude extract.

3) Performing supercritical carbon dioxide extraction on the crude liquid prepared in the step 1), wherein the specific conditions of the supercritical carbon dioxide extraction are T2 ℃ and P1Mpa, and collecting the extract.

4) Pulverizing Polygoni Multiflori radix, semen Cuscutae and fructus Ligustri Lucidi into 200 mesh fine powder, adding 32 parts of 95% ethanol, processing for 24 hr, and mixing the supernatant with the extract to obtain plant extract.

5) Standing and extracting crocodile gall and 95% alcohol according to the mass ratio of M1 for t2 hours, and diluting by 10 times to obtain crocodile gall extracting solution.

6) Stirring and mixing the crocodile bile extract and the plant extract for t3min to obtain a first mixed solution.

7) And (3) homogenizing and emulsifying the alligator oil and the first mixed solution at T3 ℃ in vacuum for T4min, and irradiating by using cobalt 60 to obtain the black hair compound.

TABLE 1

Examples	1	2	3	4	5
						T1/℃	100	110	110	120	120
t1/h	1	2	1.5	1.5	2
						T2/℃	37	31	39	40	39
P1/Mpa	33	30	23	30	18
						M1	1:30	1:27	1:25	1:25	1:27
t2/h	14	18	20	24	24
						t3/min	30	35	40	45	45
T3/℃	72	75	78	75	81
						t4/min	50	45	45	55	60

TABLE 2

Comparative example

Comparative example 1

1) Weighing 22 parts of black beans, 18 parts of black sesame, 14 parts of safflower, 16 parts of cistanche, 24 parts of purslane and 22 parts of semen cuscutae, mixing with 100 parts of water, boiling for 1.5 hours at 120 ℃, and filtering to obtain a crude extract.

2) Extracting the crude liquid prepared in the step 1) by supercritical carbon dioxide, wherein the conditions of the supercritical carbon dioxide extraction are as follows: collecting extract at 40 deg.C under 30 Mpa.

3) Pulverizing Polygoni Multiflori radix, semen Cuscutae and fructus Ligustri Lucidi into 200 mesh fine powder, adding 32 parts of 95% ethanol, processing for 24 hr, and mixing the supernatant and the extract to obtain plant extract as hair blackening composition.

Comparative example 2

1) Weighing 10 parts of crocodile gall extract and 7 parts of crocodile oil, mixing, homogenizing and emulsifying at 75 ℃ in vacuum for 45min, and irradiating by cobalt 60 to obtain the hair blackening compound.

The hair blackening compositions obtained in examples 1 to 5 and comparative examples 1 to 2 were subjected to a population test. The using method of the crowd test comprises a using method of a professional store and a daily using method of home furnishing.

The using method of the professional store comprises the following steps:

the first step is as follows: the hair was cleaned with shampoo and the scalp was wiped dry.

The second step is that: directly and uniformly smearing 3-10 g of black hair compound liquid on the white hair according to the hair quantity, and completely covering the root of the white hair.

The third step: introducing with physical vapor instrument for 20 min.

The fourth step: steaming for 15min, stopping steaming for 3min, and washing with clear water.

The fifth step: the fingers were gently massaged for 10 minutes.

And a sixth step: after 3 hours, the shampoo is used for normally caring hair.

The household use method comprises the following steps:

the first step is as follows: after the head is cleaned, the scalp is wiped dry,

the second step is that: and (3) applying 3-10 g of hair compound liquid to the white hair part, and completely covering the root part of the white hair.

The third step: massaging for 15 minutes or combing 150 times along the middle axis and two sides of the head with a comb to make the product fully absorbed.

A fourth step of: cleaning with shampoo for 25min after combing.

If the method is used only in the home, the medicine is used at least 3 times per week.

Cases of disease

Case 1: zheng lady, a bank clerk, age 46, non-hereditary white hair, and no effect of using imperial hall's product once, later used the black hair compound of example 1, going to professional store three times a week, and used once a day at home according to the household procedure for the remaining four days a week. The effect is obvious after 60 days of use, and the hair root and the black hair are increased by 10 percent.

Case 2: aged women, a company accounting, 42 years old, non-hereditary white hair, no other product used, the black hair compound of example 1 was used, three professional shops per week, and once a day at home for the remaining four days per week according to a household procedure. The effect is obvious after 75 days of use, and the hair root and the black hair are increased by 12 percent.

Case 3: weekly women, a security officer, age 42, non-hereditary white hair, no other product used, use the black hair compound of example 1. Go to professional stores three times a week, and use once a day at home for the remaining four days a week according to a household schedule. The effect is obvious after 89 days of use, and the hair root and the hair blackness are increased by 13 percent.

Case 4: mr. Zhao, insurance company employee, 38 years old, non-hereditary white hair, no other product used, the black hair compound of example 2 was used. Go to professional stores twice a week, and use once a day at home according to the household procedure for the remaining five days a week. After 60 days of use, the effect is remarkable, and the hair root and the hair blackness are increased by 18 percent.

Case 5: mr. xu, West penguin and old age, 38 years old, non-hereditary white hair, no other product used, the black hair compound of example 2 was used. Go to professional stores twice a week, and use once a day at home according to the household procedure for the remaining five days a week. After 80 days of use, the effect is remarkable, and the hair root and the black hair are increased by 20 percent.

Case 6: mr. Weekly, a company's staff, 40 years old, non-hereditary white hair, no other product used, the black hair compound of example 2 was used. Go to professional stores twice a week, and use once a day at home according to the household procedure for the remaining five days a week. After 90 days of use, the effect is remarkable, and the hair root and the black hair are increased by 20 percent.

Case 7: mr. Ten, a company with a sales headquarters, age 44, non-hereditary white hair, no other product used, the black hair compound of example 3. Go to professional stores twice a week, and use once a day at home according to the household procedure for the remaining five days a week. After 30 days of use, the effect is remarkable, and the hair root and the hair blackness are increased by 28 percent.

Case 8: mr. Zhang, auxiliary company of medical instruments, 51 years old, non-hereditary white hair, Zhangguang 101 hair tonic, hair pose original skin nutrient solution, Yuanbai kusnezoff monkshood hair liquid 3 kinds of products, but ineffective. The black hair compound of example 3 was used, 3 times daily, and irradiated with red light to enhance absorption. After 45 days of use, the effect is remarkable, and the hair root and the black hair are increased by 33 percent.

Case 9: mr. zingiberis, counsel staff, age 39, non-hereditary white hair, no other product used, the black hair compound of example 3 was used. Go to professional stores twice a week, and use once a day at home according to the household procedure for the remaining five days a week. After 60 days of use, the effect is remarkable, and the hair root and the black hair are increased by 39 percent.

Case 10: miss Zhu, a research and development worker of a company, age 44, non-hereditary white hair, no other product used, the black hair complex of example 4 was used. Go to professional stores twice a week, and use once a day at home according to the household procedure for the remaining five days a week. After 90 days of use, the effect is remarkable, and the hair root and the black hair are increased by 52 percent.

Case 11: miss, accounting, 40 years old, non-hereditary white hair, no other product used, the black hair compound of example 4 was used. Go to professional stores twice a week, and use once a day at home according to the household procedure for the remaining five days a week. After 90 days of use, the effect is remarkable, and the hair root and the black hair are increased by 56 percent.

Case 12: mr. Ling, design company employee, 40 years old, non-hereditary white hair, no other products used, the black hair compound of example 5. Go to professional stores twice a week, and use once a day at home according to the household procedure for the remaining five days a week. After 75 days of use, the effect is remarkable, and the hair root and the hair blackness are increased by 44 percent.

Case 13: mr. zingiber, design company staff, 32 years old, non-hereditary white hair, no other products used, the black hair complex of example 5 was used. Go to professional stores twice a week, and use once a day at home according to the household procedure for the remaining five days a week. After 75 days of use, the effect is remarkable, and the hair root and the black hair are increased by 45 percent.

Case 14: mr. Sun, saler in medical instruments, age 45, non-hereditary white hair, no other product used, the black hair compound of comparative example 1 was used. Go to professional stores twice a week, and use once a day at home according to the household procedure for the remaining five days a week. After 60 days of use, the effect is not obvious, and the hair root and the hair blackness are increased by 3 percent.

Case 15: dawn, webstore owner, 38 years old, non-hereditary white hair, no other product used, the black hair compound of comparative example 2 was used. Go to professional stores twice a week, and use once a day at home according to the household procedure for the remaining five days a week. After 60 days of use, the effect is not obvious, and the hair root and the black hair are increased by 4 percent.

Case 16: miss, a company, after their cashier, 36 years old, non-hereditary white hair, no other product used, the black hair compound of comparative example 1 was used. Go to professional stores twice a week, and use once a day at home according to the household procedure for the remaining five days a week. After 60 days of use, the effect is not obvious, and the hair root and the black hair are increased by 4 percent.

Case 17: mr. Guo, medical Instrument company sales headquarters, 38 years old, non-hereditary white hair, no other products used, the black hair compound of comparative example 2 was used. Go to professional stores twice a week, and use once a day at home according to the household procedure for the remaining five days a week. After 80 days of use, the effect is not obvious, and the hair root and the hair blackness are increased by 5 percent.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. The hair blackening compound is characterized by being prepared from the following raw materials in parts by mass:

2. The hair blackening compound of claim 1, wherein the hair blackening compound is made from the following raw materials:

3. The hair blackening compound of claim 1, wherein the hair blackening compound is made from the following raw materials:

4. The hair blackening compound of claim 1, wherein the hair blackening compound is made from the following raw materials:

5. A hair blackening composition comprises a plant extract, an alligator oil and an alligator gall extract;

the plant extract comprises the following raw materials, by mass, 18-25 parts of polygonum multiflorum, 18-23 parts of black beans, 8-20 parts of black sesame, 5-15 parts of safflower, 6-18 parts of cistanche, 18-25 parts of purslane, 10-23 parts of semen cuscutae and 4-12 parts of glossy privet fruits; 3-12 parts of alligator oil; 2-8 parts of crocodile gallbladder.

6. The method for preparing the hair blackening complex according to claim 5, comprising the steps of:

and mixing the plant extract, the crocodile oil and the crocodile gall extract to obtain the hair blackening compound.

7. A method for preparing a hair blackening composition according to any of claims 1 to 4, which comprises the steps of:

mixing plant extracts obtained from the raw materials of the polygonum multiflorum, the black beans, the black sesame, the safflower, the cistanche, the purslane, the glossy privet fruit and the dodder seed, crocodile gall extracts obtained from the raw materials of crocodile gall and crocodile oil to obtain a black hair compound.

8. The method for preparing a hair-blackening complex according to claim 7, wherein the plant extract is prepared by the steps of:

performing supercritical carbon dioxide extraction on the black beans, the black sesame, the safflower, the cistanche and the purslane to obtain a first extract; and

soaking the polygonum multiflorum, the semen cuscutae and the glossy privet fruit in 95% alcohol, taking supernate and mixing the supernate with the first extract to obtain a plant extract.

9. Use of the black hair complex of any one of claims 1 to 5 in a wash care product or in the preparation of a medicament for black hair.

10. A hair cream comprising the hair blackening composition according to any one of claims 1 to 5.