KR101512606B1 - Cosmetic composition for improving wrinkle and atopic dermatitis and moisturizing - Google Patents

Abstract

The present invention relates to a cosmetic composition improving wrinkle and atopic dermatitis and moisturizing skin. The present invention includes alligator oil as an active ingredient. The present invention is capable of including 0.1 to 20 wt % of alligator oil with respect to the 100 wt % of total composition and is capable of including saturated fatty acid, monounsaturated fatty acid, and polyunsaturated fatty acids. Moreover, the alligator oil can be oil extracted from a crocodylus porosus.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for improving wrinkles and atopy and for moisturizing the skin. BACKGROUND OF THE INVENTION 1. Field of the Invention [0001]

The present invention relates to a functional cosmetic composition which improves wrinkles and atopic dermatitis and has a skin moisturizing effect.

As society develops, a healthy and laid-back life is demanded, and people's interest in skin care is rising.

The wrinkles can be said to be the changes in the shape and structure of the skin that are locally formed and fixed at the site of chronic deformation due to the muscle movement. Not only the physiological changes but also the changes in the internal structure of the skin, It is also important to study the mechanism of formation to understand the actual wrinkling mechanism.

In this regard, changes in internal structures of the skin due to photoaging have been reported, such as epidermal and dermal hyperplasia, abnormal accumulation of elastic fibrous materials, or changes in the steric structure of elastic fibers. In addition, collagen fibers, which are the main constituents of the skin structure, are known to be microfibrillated with collagen fibers and weakened in fiber form (Zheng P. and Kligman LH, uvA-induced ultrastructural changes in hairless mouse skin: A comparison to uvB-induced damage., J. Invest. Dermatol., 100, 194-199 (1993)).

However, it is known that bundles of collagen fibers, which are bundled and formed, have a great influence on the toughness and the like of the skin through research on the wound treatment process of the skin. However, the structure change due to photoaging, changes in skin properties, Have not been sufficiently examined to date.

The interest of skin care has been increasing with age, and as the age of 25 years old, it is observed that age and fine wrinkles are increasingly aging at the age of 35, and the increase of spots, freckles, fine wrinkles and skin darkness increase rapidly After passing through age, large wrinkles begin to appear, and the movement of the skin generally falls through about 55 years of age. The cause of aging is known to be caused by a decrease in water retention ability and sebaceous secretion ability, accumulation of harmful substances on the skin due to drying, ultraviolet rays, oxidation, and the reduction of skin cell movement occurring inside the skin. Some skin-care methods that can cope with changes in age are to improve water retention, prevent the removal of harmful substances from the skin, and increase skin cell activity.

In addition, atopic dermatitis is caused by increased immunoglobulin E, lymphocyte increase, increase of mast cell, increase of pH, increase of transdermal water loss, reduction of ceramide due to expansion of cells and cells, etc. And the barrier function of the epidermis is deteriorated. In addition, skin diseases such as atopic dermatitis, a recurrent chronic dermatitis with a severe sense of well-being, are still known to be an incurable disease to be solved by mankind together with cancer, AIDS and dementia. In other words, according to approach by allergic reaction, access to skin barrier function, approach by neurological stress, 1) detection of cause and exacerbation factor, removal of cause, 2) correction of skin function by skin care, 3) Anti-steroids, antivitamins, and antiallergic drugs have been selected for drug treatment, but the cause of atopic dermatitis has not yet been clarified. .

Atopic dermatitis is called as fever, and it is accompanied by allergic diseases such as asthma and hay fever which are chronic diseases of skin. Allergy is one of the factors that can cause atopic dermatitis. In other words, when a skin reaction test for a protein antigen is performed, urticaria reactions to a large number of antigens appear well, and when the body is exposed to these antigen, anaphylactic shock, urticaria and angioedema, It causes familial reactions such as difficulty, nasal congestion, runny nose, sneezing, conjunctival hyperemia and edema, vomiting, diarrhea, abdominal pain, and other types of immediate reactions in the skin, respiratory, eye, and mucous membranes. What can aggravate atopic dermatitis include skin irritation caused by dry skin, ambient temperature and humidity, severe exercise and sweat, wool and fibers, food, drugs, house dust, animal hair, irritant chemicals, . In addition, it has been reported that the germs and fungi present in the skin are exacerbated (Sugimoto et al., 45th Japanese Allergy Society, Lecture Book No.381p), and deeply involved in atopic dermatitis (Kanda et al., The 51st Annual Meeting of the Japanese Society of Allergy, Lecture Book. No. 20).

These atopic dermatitis is recurrent chronic dermatitis with severe itching. It is reported that about 10 to 15% of children have atopic dermatitis, and 90% of them are self-healing within 5 years. In addition, these atopic dermatoses are generally improved in adulthood, and about 30 to 40% of the dermatitis do not recur in appearance, while the rest are known to cause severe dermatitis such as skin irritation by skin irritation, This proportion is increasing along with the progress of industrialization and the increase of environmental pollution.

In addition, dry and sensitive skin has low water retention ability and resilience, and it is likely to cause skin keratinization and itching. In severe cases, it develops as dermatitis such as atopic dermatitis. Other causes of atopic dermatitis should be cured by other means, mostly with some overlapping. In particular, when atopic fever can not be diverted, it can cause rash and itching by accumulating in the skin. It is a skin disease that the immune function is extremely reduced by the use of steroid ointment and antihistamine.

Several compositions have been proposed to treat skin dryness and itching such as atopic dermatitis and the like. However, most of these pharmaceutical compositions contain a steroid agent or an antibiotic as an active ingredient. Therefore, when used for long-term skin, Or cause serious side effects such as causing increase or expansion of the thickness of the stratum corneum. In addition, cosmetic compositions containing white roots, cloves, gardenia, sculptors, herbicides and the like as active ingredients are known to be associated with cosmetic compositions for atopic dermatitis. However, most of these plant natural products themselves are heated by heating at high temperature or using organic solvents Therefore, there is a problem that the active ingredient is destroyed and irritation occurs to the skin. Further, there are more problems due to the preservative used for preventing the corruption of the extracts.

 Zheng P. and Kligman L. H., uvA-induced ultrastructural changes in hairless mouse skin: A comparison to uvB-induced damage, J.Invest.dermatol., 100, 194-199 (1993)  Sugimoto et al. The 45th Japanese Allergy Society, Lecture Collection No.381p  Kanda et al. The 51st Annual Meeting of the Japan Allergy Association. No.20

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a cosmetic composition containing crocodilla oil as an active ingredient to improve wrinkles and atopic dermatitis and to obtain skin moisturizing effect.

These and other objects and advantages of the present invention will become more apparent from the following description of a preferred embodiment thereof.

The above object is achieved by a cosmetic composition characterized by containing crocodilla oil as an active ingredient.

Preferably, the crocodile oil may comprise 0.1 to 20% by weight based on 100% by weight of the total composition.

Preferably, the crocodile oil may comprise saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids.

Preferably, the saturated fatty acid may comprise at least one of palmitic acid, stearic acid and myristic acid.

Preferably, the monounsaturated fatty acid may comprise at least one of oleic acid and palmitoleic acid.

Preferably, the polyunsaturated fatty acids are selected from the group consisting of linoleic acid, gamma-linolenic acid, alpha-linolenic acid, docosahexaenoic acid and icosatrienoic acid eicosatrienoic acid).

Preferably, the crocodile oil may be the oil extracted from the sea crocodile (Crocodylus Porosus).

Preferably, the cosmetic composition may be a formulation selected from the group consisting of softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack and shampoo.

The cosmetic composition of the present invention includes crocodile oil, proliferates fibroblasts, improves wrinkles and atopic dermatitis, and has excellent skin moisturizing effect. In addition, it does not irritate the skin, so it has excellent safety, and there are no side effects due to use, so anyone of all ages can use it safely.

However, the effects of the present invention are not limited to the above-mentioned effects, and other effects not mentioned can be clearly understood by those skilled in the art from the following description.

Hereinafter, the present invention will be described in detail with reference to examples of the present invention. It will be apparent to those skilled in the art that these embodiments are merely illustrative of the present invention in order to more particularly illustrate the present invention and that the scope of the present invention is not limited by these embodiments .

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, including definitions, will control.

The cosmetic composition according to one aspect of the present invention contains croc oil as an active ingredient.

In one embodiment, crocodile oil is extracted and purified from adipose tissue of a crocodile. Crocodile oil has been known to treat many intractable and chronic skin diseases for centuries. In modern clinical trials Crocodile oil has been proven to have skin permeability, antibacterial activity and blood circulation enhancement. , Burns, eczema, abrasions, cuts, ulcers, pressure ulcers, contusions, and acne. Crocodile oil has little scent and very little skin irritation. It is suitable for all ages. It can be applied immediately to the skin, and is especially effective in improving eczema, wrinkles and atopic dermatitis.

In one embodiment, the crocodile oil is included in an amount of 0.1 to 20% by weight, preferably 1 to 10% by weight, based on 100% by weight of the total amount of the cosmetic composition. If the content of the crocodile oil is less than 0.1% by weight, the effect of improving wrinkles and atopic dermatitis and the skin moisturizing effect are hardly obtained. When the amount of the allicum oil is more than 20% by weight, the effect of improving wrinkles and atopic dermatitis, It is inefficient.

In one embodiment, the crocodile oil comprises saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids.

In one embodiment, the saturated fatty acid comprises at least one of palmitic acid, stearic acid, and myristic acid.

In one embodiment, the monounsaturated fatty acid comprises at least one of oleic acid and palmitoleic acid.

In one embodiment, the polyunsaturated fatty acids are selected from the group consisting of linoleic acid, gamma-linolenic acid, alpha-linolenic acid, docosahexaenoic acid, And an acid (eicosatri e noic acid).

In one embodiment, the alligator oil may use oils extracted from alligators, crocodiles, and alligators belonging to the genus Gaviella, preferably using oils extracted from Crocodylus Porosus.

In one embodiment, the cosmetic composition may be used in all cosmetics relating to the effect of improving wrinkles and atopic dermatitis, moisturizing effect, and selected from the group consisting of softening agents, nutritional lotion, nutritional cream, massage cream, essence, pack and shampoo Or < / RTI >

The cosmetic composition according to one aspect of the present invention may include a surfactant, an emulsifier, a skin conditioner, an increasing agent, and a pH adjuster.

In one embodiment, the surfactant is at least one of polysorbate-60 and sorbitan stearate.

In one embodiment, the emulsifier is at least one of stearine acid, cetostearyl alcohol, and cetyl octanoate.

In one embodiment, the skin conditioner is at least one of squalane and glycerin.

In one embodiment, the builder is a carbomer.

In one embodiment, the pH adjusting agent is triethanol amine.

Hereinafter, the structure and effect of the present invention will be described in more detail with reference to examples and comparative examples. However, this embodiment is intended to explain the present invention more specifically, and the scope of the present invention is not limited to these embodiments.

Crocodile oil used in the examples according to the present invention is generally commercially available and purchased from crocodile oil (Australia) for artificial farms which does not violate the CITES (Endangered Species Convention) Respectively.

[Examples 1 to 5]

Ingredients listed in Table 1 were placed in a homomixer, and the whole composition was adjusted to 100 g with purified water and stirred, followed by heating and cooling to prepare uniformly mixed cosmetic compositions, which were Examples 1 to 5.

[Comparative Examples 1 and 2]

The ingredients listed in Table 1 were placed in a homomixer, and the whole composition was adjusted to 100 g with purified water, stirred and heated and cooled to prepare a uniformly mixed cosmetic composition, which was designated Comparative Examples 1 and 2. In Comparative Example 1, no croc oil was added.

[Table 1]

[Experimental Example 1: Comparison test of fibroblast proliferation ability]

Human fibroblasts derived from human skin were used to test their cell proliferating ability. Fibroblasts were used to proliferate cells stored in liquid nitrogen. The fibroblasts cultured in T-75 flasks were washed 2 to 3 times with PBS, adhered cells were removed using trypsin, and the cells were collected by centrifugation (Heraeus, Labofuge 400R) at 1,500 rpm for 5 minutes. After centrifugation, the supernatant was discarded and 10 ml of DMEM (10% FBS) was added to the precipitated cells. Cells were counted using a hemocyte counter and cells of 10 ⁴ ea / well were dispensed in 200 μl of each well of 96 wells. When 50% of the cells were grown at 37 ° C and 5% CO ₂ , the medium was exchanged with fresh DMEM (FBS 10%). , And the cells were cultured at 37 ° C and 5% CO ₂ at each time point.

MTT (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide, Sigma) was dissolved in PBS to 2 mg / ml and stored in the refrigerator. MTT reagent / Ml in PBS) and incubate for another 2 hours. Discard the supernatant, add 150 μl of DMSO, and mix well. After all steps, measure at 560 nm using an ELISA leader (μQuant, Bio-Tek instrument INC.) (Reference: 650 nm)

The effect of crocodile oil showed cell proliferation effect of about 85% when compared with 0% control at 48 hours after treatment at 0.1%.

[Experimental Example 2: Skin elasticity measurement test]

Sixty healthy women (mean age 29.5 years) aged 20 years or older at a temperature of 22 to 24 DEG C and a relative humidity of 55% were divided into six groups and Example 3 and Comparative Example 1 were compared to groups of six eyes And skin elasticity was measured using a skin elasticity tester (Cutometer SEM 575, C + K Electronic Co., Germany) after 12 weeks of application (2 times / day). The experimental results are shown in the following Table 2 as ΔR5 value [R5 (12 weeks) -R5 (0 weeks)], which is an average value of each test group of Cutometer SEM 575. R5 value is a value It shows that the elasticity is better than the ground.

The degree of improvement was calculated by the following equation (1).

(1)

Improvement degree (%) = (? R5 / before application) X 100

[Table 2]

According to the above Table 2, when the crocodile oil-containing Example 3 was used, skin elasticity was increased by 42.68% as compared with Comparative Example 1. Thus, it can be seen that the cosmetic composition containing crocodile oil effectively enhances skin elasticity when using the constituent of NMF (Natural Moisturizing Factor) (when a cosmetic composition containing no crocodile oil is used).

[Experimental Example 3: Experimental measurement of skin moisture]

The subjects were divided into 7 groups of 10 healthy subjects (average age 29.5 years) who were healthy 22 ~ 24 ℃, 55% relative humidity and no air flow. The cosmetic composition of Example 4 and Comparative Example 1 was applied to the whole face for about 6 weeks twice a day around the eyes of each group. The moisture content of the ball area was measured before and after coating using Skicon-200 (IBS Co. Japan), and the persistence of the moisturizing effect was measured. The results are shown in Table 3 as average values for each test group.

The degree of improvement was calculated according to the following equation (2).

(2)

[Table 3]

According to the above Table 3, it can be seen that when the cosmetic composition comprising 0.1 to 20% by weight of croc oil in the cosmetic composition is used, the skin moisturizing effect is excellent.

[Experimental Example 4: Experiment for Measuring Water Loss on Skin Surface]

In the above Examples 1 to 5 and Comparative Example 1, the water loss of the hard skin was measured and compared. The amount of water loss in the epidermis was measured in 10 non - lesional adults (20 ~ 30) and 10 lesions (20 ~ 30). The experimental method is as follows. The skin conductivity was measured using a skin integrator (MPA-5, Courage + Khazaka, Cologne, Germany) at room temperature and humidity conditions (24 ° C and 40% As a default, 9 regions were arbitrarily selected and the changes of skin conductivity were measured at 0 weeks, 4 weeks, and 8 weeks after application of the uncoated region, Examples 1 to 5 and Comparative Example 1 at 2 mg / The amount of water loss on the skin surface was evaluated and the results are shown in Table 4.

[Table 4]

As can be seen from Table 4, in Examples 1 to 5, no significant difference was observed in the water loss of the skin surface, but it was confirmed that the amount of water loss on the skin surface was significantly reduced in the lesion. Therefore, when a cosmetic composition containing crocodile oil is used, the ability to suppress moisture loss on the skin surface tends to be greatly increased.

[Experimental Example 5: Human Patch Experiment]

In order to confirm that the cosmetic composition including crocodile oil has no skin irritation and other side effects, a patch test was performed on the human body using the cream formulation of the cosmetic composition of Example 2, Example 5 and Comparative Example 1.

The test was carried out by using a finn chamber in the back of a healthy adult male and female. The skin was placed in an aluminum disc or put on a paper disc and treated with 40 μl of the sample. The skin was scanned with a scanpore tape After 24 hours, the patches were removed, and the results were judged after 4 hours.

The results are expressed in terms of the degree of erythema and edema, and the criteria are based on the criteria of the International Contact Dermatitis Research Group (ICDRG) (Wooding et al, 1967; Rietschell, 1982; Fischer & Maibach, 1984; Aberer et al, 1993).

0: No flare

1: erythema

2: redness and papules

3: redness, pus and vesicles

4: severe edema and minor depression

Based on the score determined according to the above criteria, the degree of stimulation was calculated according to the following equation (3), and the results are shown in Table 5 below.

(3)

(%) = (Reactivity showing positive / reactivity of own population) × 100

[Table 5]

As can be seen from the results of Table 5, there were no skin inflammation or other side effects in Examples 2 and 5, unlike Comparative Example 1. [

[Experimental Example 6: Experiment for Measuring Moisturizing Effect]

A 2 × 2 cm2 area was set on both arms of a healthy male and female subjects. An aluminum foil was placed on the arm so that only the test site was irradiated with ultraviolet rays, and a UVGL-58 (Upland, USA) And irradiated three times in a row once a day at a dose of 0.5 × 10 ^-7 erg / cm 3 per day. Before the irradiation, the test site was washed well with a 70% aqueous solution of alcohol. After irradiation, Examples 1 to 5 containing a moisturizing peptide were applied to the UV-irradiated region three times a day for 3 weeks. After three weeks of application, the degree of pigmentation was judged visually, and the degree of inhibition of pigmentation compared to Comparative Example 1 was evaluated in three stages: effective, normal, and no effect. The results are shown in Table 6.

[Table 6]

As can be seen from Table 6, it was confirmed that the cosmetic composition containing 0.1% by weight to 20% by weight of crocodile oil (Examples 1 to 5) had a much better moisturizing effect than Comparative Example 1, When it is more than 20% by weight (Comparative Example 2), it can be confirmed that an excellent effect can not be obtained compared with an increased use amount.

[Experimental Example 7: Measurement of change in pH value of skin surface]

To measure the pH change of the skin, 20 healthy male and female subjects were measured by using Skin pH Meter 900PC.

[Table 7]

For healthy skin, the pH is usually maintained at 5 to 5.5, while when using Examples 1 to 5, the pH was maintained at 5 for every 8 weeks. However, in the case of Comparative Example 1, the pH did not reach 5, and there was almost no change compared with the initial pH.

[Experimental Example 8: Experiment to confirm the improvement effect of atopic dermatitis using the in-vivo evaluation method]

In order to evaluate the improvement effect of atopic dermatitis according to Example 3 above, clinical tests were conducted on infants 1 to 10 years old and children 30 years or younger who have atopic skin. Atopic dermatitis was classified into three stages according to the symptom severity, and the ratio of severity of atopic disease was severely: normal: weak = 1: 2: 2 ratio. At this time, Example 3 prepared as a cream formulation was gently stretched by 0.5 to 2 g / s twice twice a day according to the site. After 8 weeks of use, the subjects were assessed with the following five points of satisfaction scale (5 points of satisfaction, 4 points of satisfaction, 3 points of dissatisfaction, 2 points of dissatisfaction, 1 point of dissatisfaction). The results are shown in Table 8.

[Table 8]

As can be seen from Table 8, when Example 3 was used, it was confirmed that it exerts excellent effects on keratin skin, itching, and inflammation, which are typical symptoms of atopic dermatitis.

It is to be understood that the present invention is not limited to the above embodiments and various changes and modifications may be made by those skilled in the art without departing from the spirit and scope of the invention.

Claims (8)

A crocodile oil containing a saturated fatty acid, a monounsaturated fatty acid and a polyunsaturated fatty acid as an active ingredient,
Wherein the crocodile oil contains 5% by weight based on 100% by weight of the whole composition. delete delete The method according to claim 1,
Wherein the saturated fatty acid comprises at least one of palmitic acid, stearic acid, and myristic acid. The method according to claim 1,
Wherein the monounsaturated fatty acid comprises at least one of oleic acid and palmitoleic acid. The method according to claim 1,
The polyunsaturated fatty acids may be selected from the group consisting of linoleic acid, gamma-linolenic acid, alpha-linolenic acid, docosahexaenoic acid and eicosatrienoic acid. Wherein the at least one cosmetic composition comprises at least one of the following ingredients: The method according to claim 1,
The crocodile oil is an oil extracted from Crocodylus Porosus, which is a cosmetic composition for improving atopic dermatitis. The method according to claim 1,
Wherein the cosmetic composition is a formulation selected from the group consisting of softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, and shampoo.