CN115006468B

CN115006468B - Compound Chinese herbal medicine hair-growing liquid and preparation method thereof

Info

Publication number: CN115006468B
Application number: CN202210752889.3A
Authority: CN
Inventors: 崔宁; 上官静波; 许宁侠; 吴康
Original assignee: Xian International University
Current assignee: Guangzhou Huaxia Biopharmaceutical Co ltd
Priority date: 2022-06-28
Filing date: 2022-06-28
Publication date: 2023-06-06
Anticipated expiration: 2042-06-28
Also published as: CN115006468A

Abstract

The invention provides a compound Chinese herbal medicine hair growth liquid, which comprises a phase A, a phase B and a phase C, wherein the total mass percentage of the liquid is as follows: the phase A comprises: 1, 3-butanediol 12 percent glycerol 5 percent transparent xanthan gum 0.1 percent deionized water to 100 percent; the phase B comprises: 0.5% of compound Chinese herbal medicine extract 30% panthenol 2% nicotinamide; the phase C comprises: 0.5% of phenoxyethanol. Proved by verification, the compound Chinese herbal medicine hair-growing liquid can promote the hair regeneration speed, so that the grown hair is more smooth and glossy. The invention also provides a preparation method of the compound Chinese herbal medicine hair tonic.

Description

Compound Chinese herbal medicine hair-growing liquid and preparation method thereof

Technical Field

The invention relates to a compound traditional Chinese medicine preparation, in particular to a compound Chinese herbal medicine hair-growing liquid.

Background

According to statistics, the existing alopecia patients in China reach 1.68 hundred million people, wherein androgenetic alopecia and alopecia areata are taken as main alopecia types, and the total loss is more than 90%. Alopecia affects appearance, brings pressure and trouble to patients, and patients suffering from alopecia are also prone to nail defect, and complications such as eye diseases, vitiligo, hypothyroidism and the like are affected. Hair loss patients are growing year by year and tend to be younger, the market demand of hair growth products is increasing, and the market value can reach about 100 hundred million. In the treatment of alopecia, the western medicines currently used clinically are finasteride and minoxidil. However, the cure rate of the two medicines is not high, the medicines are easy to relapse after stopping taking, and side effects with different degrees can appear after long-term use, such as scalp itching, hair dryness, dizziness, cold sweat, anorexia, myocardial infarction and the like. Therefore, the development of safer and more efficient drugs for treating alopecia becomes a research hotspot.

The invention aims at providing a compound Chinese herbal medicine hair-growing liquid.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention provides a compound Chinese herbal medicine hair tonic which can effectively promote hair growth and improve alopecia.

In order to achieve the purpose, the compound Chinese herbal medicine hair growth liquid comprises an A phase, a B phase and a C phase, and the weight percentages of the total weight are as follows:

the phase A comprises:

1, 3-butanediol 12%

Glycerol 5%

Transparent xanthan gum 0.1%

Deionized water to 100%;

the phase B comprises:

30% of composite Chinese herbal medicine extract

Panthenol 2%

0.5% of nicotinamide;

the phase C comprises:

0.5% of phenoxyethanol.

Further, the composite Chinese herbal medicine extract is extracted from mixed Chinese herbal medicine, and the mixed Chinese herbal medicine comprises the following components in parts by weight:

prepared fleece flower root 6 parts

6 parts of red sage root

Ligusticum wallichii 6 parts

3 parts of biota orientalis

3 parts of licorice

Black sesame 3 parts

Prepared rehmannia root 6 parts

And 6 parts of glossy privet fruit.

Further, the composite Chinese herbal medicine extract is obtained by extracting mixed Chinese herbal medicines with ethanol.

Further, the composite Chinese herbal medicine extract also comprises:

melittin 200 ng/ml

Sodium thiosulfate 4% (w/w).

The invention also provides a preparation method of the compound Chinese herbal medicine hair tonic, which comprises the following steps:

s1: respectively weighing prescribed amounts of Chinese medicinal materials including prepared fleece flower root, red sage root, szechuan lovage rhizome, cacumen biotae, liquorice, black sesame, prepared rehmannia root and glossy privet fruit, mixing, cleaning for 2-3 times by using clear water, and airing for standby;

s2: adding 75% ethanol into the above mixed Chinese medicinal materials, ultrasonic extracting for 30min, filtering, collecting filtrate, and repeating for one time;

s3: combining the two filtrates, concentrating under reduced pressure, mixing, centrifuging, and filtering to obtain compound Chinese herbal medicine extract;

s4: accurately weighing each substance in the phase A, placing in a beaker with proper size, heating for 30min in a constant-temperature water bath kettle with temperature of 85 ℃, taking out, stirring with a glass rod, cooling to 40 ℃, sequentially adding the phase B and the phase C, and fully mixing;

s5: adding melittin and sodium thiosulfate, and mixing to obtain the final product.

Prepared fleece-flower root and glossy privet fruit nourish yin and tonify kidney, develop hair follicles and grow hair without opening capillary blood supply of papilla parts. The active ingredients of the cacumen biotae mainly comprise essential oil and Huang Sizhi, and the active ingredients of the cacumen biotae have obvious anti-inflammatory effect and have inhibition effect on staphylococcus aureus, escherichia coli and tetranectia coccus. Tannins in cacumen Platycladi have antioxidant, free radical scavenging and antitumor effects. The alcohol extract of biota orientalis and its volatile oil can delay hair loss to a certain extent. Fructus Ligustri Lucidi is fruit of Ligustrum lucidum karst of Ligustrum of Oleaceae, has cool nature, dry taste, slightly bitter taste, and has effects of improving eyesight, blackening hair, and nourishing liver and kidney, and is commonly used for treating liver and kidney yin deficiency, giddiness tinnitus, soreness of waist and knees, premature gray hair, etc. The fructus Ligustri Lucidi ethanol extract has effect in promoting hair growth for alopecia caused by androgen. The prepared rehmannia root has the effects of nourishing yin and supplementing blood, tonifying kidney and promoting hair growth, nourishing yin and supplementing blood, activating blood and dissolving stasis, clearing heat and promoting diuresis, and dispelling wind and removing dampness.

The compound Chinese herbal medicine hair-growing liquid has the effect of promoting hair growth, can promote the hair regeneration speed, and enables the hair to be more smooth and glossy.

Drawings

The invention is further described and illustrated below with reference to the accompanying drawings.

FIG. 1 is a graph of the stepwise hair growth of mice.

FIG. 2 is a graph showing the hair growth scores of mice

Fig. 3 is a view of a section of mouse hair growth and hair follicle tissue under an optical microscope.

Description of the embodiments

The technical solution of the present invention will be more clearly and completely explained by the description of the preferred embodiments of the present invention with reference to the accompanying drawings.

Example 1: the preparation of the compound Chinese herbal medicine hair tonic comprises the following steps:

s1: respectively weighing prescribed amounts of Chinese medicinal materials including 6g of prepared fleece flower root, 6g of red sage root, 6g of szechuan lovage rhizome, 3g of biota orientalis, 3g of liquorice, 3g of black sesame, 6g of prepared rehmannia root and 6g of glossy privet fruit, mixing, cleaning for 2-3 times by clear water, and airing for standby;

s2: adding 400ml of 75% ethanol into the mixed traditional Chinese medicinal materials, ultrasonically extracting for 30min, filtering, collecting filtrate, and repeating the above process for one time;

s3: combining the two filtrates, concentrating under reduced pressure to 470ml, mixing, centrifuging, and filtering to obtain compound Chinese herbal medicine extract;

s4: accurately weighing the phase A, the phase B and the phase C, wherein the proportion of each phase is as follows in table 1:

table 1 compatibility of compound Chinese herbal medicine hair tonic

Placing all the substances in the phase A in a beaker with proper size, placing in a constant-temperature water bath kettle with the temperature of 85 ℃, heating for 30min, taking out, stirring with a glass rod, cooling to 40 ℃, sequentially adding the phase B and the phase C, and fully mixing;

s5: adding melittin to 200 ng/ml concentration, adding sodium thiosulfate to 4% (w/w), and mixing to obtain the final product.

Example 2: the preparation of the compound Chinese herbal medicine hair growth liquid is different from the embodiment 1 in that in the step S3, the two filtrates are combined and concentrated to 235ml under reduced pressure.

Example 3: the preparation of the compound Chinese herbal medicine hair growth liquid is different from the embodiment 1 in that in the step S3, the two filtrates are combined and concentrated to 160ml under reduced pressure.

Verification of the effect of the compound Chinese herbal medicine hair tonic solution:

1. building an alopecia animal model:

after 30 male C57BL/6 mice were adaptively bred for 1 week, they were numbered by mass and randomly divided into 5 groups (blank group, MNX2%, example 1, example 2 and example 3, respectively) according to mass by Excel software, and each group of mice was photographed and recorded in detail with initial color of each back, and dehairing was performed on the backs of the mice by about 3X 4cm using dehairing paste, and the skin was pink and unbroken, confirming that the hair follicles of the mice were in resting stage. The mice are fed with the common feed at regular time and quantity every day, and each mouse can drink water freely every day with 6g of common feed.

2. Use of group mouse drugs

Minoxidil is an external medicine which is clinically used for treating alopecia at present, and the minoxidil is adopted as a positive control of an external hair growth promotion effect experiment. The specific administration methods are shown in table 2:

TABLE 2 grouping use of mouse drugs

3. Observation of Hair growth Condition

The skin and hair growth conditions of the hair removal sites of each group of mice were observed daily, and the time for changing the skin color of the hair removal site of each mouse from pink to black and the time for starting hair growth were recorded, and the effect of the drug on the hair growth cycle of the mice was evaluated. At the same time, a stepwise photograph was taken of each group of mice on

days

1, 14, 21, and 28, and the stepwise hair growth of the mice was shown in fig. 1.

As can be seen from fig. 1, after 1d, the mice in each group had no significant change and the skin was still pink; after experiment 14d, the skin color of the shaved area on the back of the mice became black, wherein in the treatment group of example 3, the skin color recovery of the mice was relatively uniform, and black fluff had grown in a part of the small area; after experiment 21d, the basic skin of each group of mice is completely blackened, but the hair growth of the compound Chinese herbal medicine hair growth liquid treatment group is more uniform than that of the blank group and the minox treatment group; after 28 days of the experiment, the hair of each group of mice was visible, wherein the hair length of the individual mice in the treatment group of example 3 was not significantly different from other parts, and the hair of the mice in the treatment group of example 2 was uniform in overall growth. From the appearance, the newly grown hair of the mice treated by the compound Chinese herbal medicine hair growth liquid is darker and brighter than the hair of other parts.

The statistics of observation of the hair growth status of the mice are shown in Table 3:

TABLE 3 skin discoloration time, long hair time schedule

The hair growth of each group of mice was scored on day 1, day 7, day 14, day 21, and day 28. Scoring criteria: 0 minutes (depilatory area skin color pink and no hair growth); 1 score (depilatory area skin color grey and hair growth less than 20%); 2 minutes (the depilatory area skin color appears gray black and the hair growth level is greater than 20% and less than 40%); 3 minutes (the skin of the dehairing area is light black and the hair growth degree is more than 40% and less than 60%); 4 minutes (the depilatory area skin color is black and the hair growth level is greater than 60% and less than 80%); 5 minutes (the hair is substantially full length in the depilation region and the extent of hair growth is greater than 80%).

As shown in fig. 2, the mouse score was linear with time. It can be clearly seen that the differences in scores of the mice in each group were not very large over the first 7 days of growth, but the scores of the mice in each group were clearly divided from day 14. The score of example 2 was significantly higher than the other groups, while the blank group was also significantly lower from day 14.

4. Determination of mouse hair length and weight and calculation of hair density

The compound Chinese herbal medicine hair tonic and minoxidil are topically applied to the back skin 28d of the mice. After the experiment is finished, killing the mice by a cervical dislocation method, taking the skin of the dehairing area of the mice, cutting 3X 4cm pieces of skin at the same position of each group of mice, scraping the hairs on the skin pieces by using a scalpel, weighing on an analytical balance, and calculating the average value of the weight of the newly-grown hairs of the mice; measuring the length of the hair by using a digital caliper and calculating the average value; and the hair density per unit area was calculated as the hair weight/hair length. At the same time, analysis is performed by a statistical method, whether significant differences exist or not is judged by inspection, and the data are expressed as mean + -standard deviation (S.D.).

The obtained collection and measurement results of the weight and length of the mouse are shown in table 4:

it can be seen that the hair growth of mice coated with the composite chinese herbal hair tonic and minoxidil was significantly increased compared to the blank group. Among them, the mice of the treatment group of example 2 had better hair length, hair weight and hair density data.

5. Histological observations

The compound Chinese herbal medicine hair growth liquid is smeared on the back of a mouse for 28 days by the method of Table 2, the cervical dislocation method of the mouse is killed after the experiment is finished, the long strip-shaped dehaired skin with the width of 1cm is cut parallel to the spinal column of the mouse, the long strip-shaped dehaired skin is lightly stuck on a cardboard, wrinkles and tweezer clamp marks are avoided, and the rat is quickly fixed by using paraformaldehyde fixing liquid for 48 hours. Subsequently, washing with water, trimming, dehydrating, immersing in wax, embedding, and slicing (6 μm) along the hair growth direction; then, the prepared baked sheet was subjected to hematoxylin eosin staining and sealing. Finally, histological changes, photographic recordings, counts and diameter measurements of the skin follicles of the mice were observed under an optical microscope. The histological appearance of the hair growth and hair follicle of the mice under light microscopy is shown in fig. 3.

As can be seen from fig. 3, the histological appearance of all drug group mice was significantly different from that of the blank group mice, and the hair follicles of mice using the compound chinese herbal medicine extract and minoxidil were significantly increased, and melanin accumulation in hair bulb was also more. The blank group has thinner skin, the subcutaneous tissue has the thinnest thickness, almost all hair follicles grow in dermis, the color in hair bulb is lighter, which indicates that the blank group mice hair is still in resting stage and melanin accumulation is less. The hair of the compound Chinese herbal medicine hair tonic treatment group is provided with a thicker fat cell layer, especially the fat cell layer in the embodiment 2 is thickest, the size of the vacuolated fat cells is uniform, the shape is clear and visible, the cells are compactly and orderly arranged along the hair growth direction, the fat cell layer extends to the dermis layer all the time, the hair growth of the group is more uniform and ideal, and meanwhile, the epidermis layer, the dermis layer and the muscle layer are orderly and compact.

In conclusion, the compound Chinese herbal medicine hair-growing liquid has the effect of promoting hair growth, can promote the hair regeneration speed, and enables the hair to be more smooth and glossy.

The above detailed description is merely illustrative of the preferred embodiments of the present invention and is not intended to limit the scope of the present invention. Various modifications, substitutions and improvements of the technical scheme of the present invention will be apparent to those skilled in the art from the description and drawings provided herein without departing from the spirit and scope of the invention. The scope of the invention is defined by the claims.

Claims

1. A compound Chinese herbal medicine hair tonic is characterized by comprising phase A, phase B and phase C, 200 ng/ml melittin and 4% sodium thiosulfate by weight percent;

in percent of the total mass, wherein:

the composition of the phase A is as follows:

1, 3-butanediol 12%

Glycerol 5%

Transparent xanthan gum 0.1%

Deionized water to 100%;

the composition of the phase B is as follows:

30 percent of composite Chinese herbal medicine extract and 2 percent of panthenol

0.5% of nicotinamide; the phase C is:

0.5% of phenoxyethanol;

the composite Chinese herbal medicine extract is prepared by extracting mixed Chinese herbal medicines with 75% ethanol, wherein the mixed Chinese herbal medicines comprise the following components: