CN109260121B - Raw material formula for preventing hair loss and growing hair - Google Patents

Abstract

A raw material formula for preventing hair loss and growing hair adopts plant extracts, and the effects of preventing hair loss and fixing hair of the plant extracts are represented by increasing the expression of hair hyalin and keratin, reshaping hair root structure, preventing scalp aging and minimizing hair loss; the expression of collagen is increased, and the environment of hair follicles is changed, so that the beautiful appearance of the hairs is restored, and the hair follicles are healthier; the extract can also help maintain the hair growth cycle, optimize cell-to-cell and cell-to-matrix adhesion, increase protein expression, and promote hair growth and hair follicle renewal.

Description

Raw material formula for preventing hair loss and growing hair

Technical Field

The invention relates to the technical field of cosmetic raw materials, in particular to a raw material formula for preventing alopecia and growing hair.

Background

In cosmetics, a plurality of raw materials can be used for preventing hair loss and nourishing hair under the permission of laws and regulations, most of the raw materials are used for preventing hair loss and nourishing hair on the basis of one single or two or three mechanisms, the functional effect of the raw materials applied to the cosmetics is not satisfactory, otherwise, the raw materials have the defects or the defects, such as unstable raw materials or difficult addition to a formula system, great confusion to a formulator, easy color change, or great irritation to the scalp, high allergy rate, no effect when the raw materials are used by a consumer, and the like. Different raw materials of different manufacturers are tried to be refined into a perfect functional raw material through different experimental researches and verifications. The whole idea is as follows: first, the alopecia is analyzed in combination with the knowledge in medical field, and the following description about the alopecia is obtained:

alopecia is a common disease in dermatology and frequently occurs. Clinically, the alopecia areata, senile alopecia and chemotherapy alopecia can be classified into seborrheic alopecia, alopecia areata, senile alopecia and the like, and the incidence rate of alopecia areata and seborrheic alopecia is the highest. The cause of alopecia is explained in many ways in the international medical community. The result is the same regardless of the etiology, namely atrophy and degeneration of hair follicles, the atrophic and degenerated hair follicles do not participate in the circulation of the body, and cannot absorb nutrients of the surrounding scalp, and the hairs do not grow naturally. Therefore, it is critical to allow the hair follicle to regain its function. The atrophy and degeneration of the hair follicle is represented by the reduction or cessation of the proliferation of hair follicle cells (mainly, Outer root follicle cells or ORS), which deteriorates the hair growth environment in the entire hair follicle, inhibits the division of hair mother cells to prevent the synthesis of hair, keeps the hair follicle in a resting stage, and gradually causes permanent (irreversible) death. With the development of science and technology, the development of western biology has more and more comprehensive understanding on the mechanism and action factors of hair growth, and the hair regrowth has feasibility by regulating the proliferation of scalp hair follicle cells through biotechnology.

Meanwhile, the traditional Chinese medicine treats alopecia on the basis of dialectical treatment, and various functional natural active ingredients are determined and applied to the field of alopecia prevention and hair growth along with the development of extraction and separation technologies on the basis of natural plant traditional Chinese medicine theory. The modern tissue engineering theory and the biological technology can explain the action mechanism of the active ingredients, so as to screen the functional ingredients for treating alopecia more effectively. In contrast, the synthetic additive has faster and more obvious effect, but also has more side effects, so the natural active hair-growing ingredient is a hot spot of current research.

The above is some discussions about alopecia, and in our country, cosmetics are prohibited to add any western medicine component to achieve the purpose of preventing alopecia. Most of the existing anti-drop raw materials aim at one or two symptoms, so the effect is not obvious in practical application. In medical terms, there are times when medication is not prescribed symptomatically.

Disclosure of Invention

In order to make up the defects of the prior art, the invention aims to provide a raw material formula for preventing hair loss and growing hair, which overcomes the defects of singleness, color change, large scalp irritation, difficult operation, unobvious effect and the like of the existing raw material for preventing hair loss and growing hair.

The technical solution adopted by the invention is as follows: the raw material formula for preventing hair loss and growing hair comprises the following components in percentage by mass: 2-8% of ginger rhizome extract, 2-8% of ginkgo leaf extract, 2-8% of ginseng extract, 2-8% of coix seed extract, 7-13% of Chinese arborvitae twig extract, 2-8% of ligusticum wallichii extract, 2-8% of pepper fruit extract, 2-8% of tea leaf extract, 2-8% of gleditsia sinensis extract, 7-13% of Chinese angelica extract, 25-11% of oligopeptide, 2-8% of salvia officinalis extract, 1-4% of menthol, 7-13% of ampelopsis japonica extract and 7-13% of radix sileris extract.

The raw material formula also comprises alcohol, hexanediol, a caprylyl hydroximic acid/glycerin caprylate/propylene glycol mixture and p-hydroxyacetophenone.

The formula consists of the following components in percentage by mass:

2-8% of ginger rhizome extract;

2-8% of ginkgo leaf extract;

2-8% of ginseng extract;

2-8% of coix seed extract;

7-13% of cacumen biotae extract;

2-8% of ligusticum wallichii extract;

2-8% of zanthoxylum bungeanum fruit extract;

2-8% of tea extract;

2-8% of gleditsia sinensis extract;

7-13% of angelica sinensis extract;

oligopeptide-25-11%;

2-8% of sage extract;

1-4% of menthol;

7-13% of ampelopsis japonica extract;

7-13% of radix sileris extract;

2-8% of alcohol;

0.1-0.3% of hexanediol;

0.5-0.7% of caprylhydroxamic acid/glyceryl caprylate/propylene glycol mixture;

0.1 to 0.3 percent of p-hydroxyacetophenone.

Preferably, the formula consists of the following components in percentage by mass:

5% of ginger rhizome extract;

5% of ginkgo leaf extract;

5% of ginseng extract;

5% of coix seed extract;

10% of cacumen biotae extract;

5% of ligusticum wallichii extract;

5% of zanthoxylum bungeanum fruit extract;

5% of tea extract;

5% of gleditschia horrida extract;

10% of angelica extract;

oligopeptide-28%;

5% of sage extract;

1% of menthol;

10% of ampelopsis japonica extractive;

10% of radix sileris extract;

5% of alcohol;

0.2% of hexanediol;

0.6% caprylhydroxamic acid/glyceryl caprylate/propylene glycol mixture;

0.2 percent of p-hydroxyacetophenone.

The ginger rhizome extract, the ginkgo leaf extract, the ginseng extract, the coix seed extract, the Chinese arborvitae twig extract, the ligusticum wallichii extract, the zanthoxylum bungeanum fruit extract, the tea leaf extract, the gleditsia sinensis extract, the angelica sinensis extract, the salvia officinalis extract, the ampelopsis japonica extract and the radix sileris extract are all water extracts.

The raw material formula can be used for cosmetic compositions.

The invention has the beneficial effects that: the invention provides a raw material formula for preventing hair loss and growing hair, which adopts plant extracts as raw materials, and the anti-hair loss and hair-fixing effects of the plant extracts are represented by increasing the expression of hair hyalin and keratin, reshaping hair root structures, preventing scalp aging and minimizing hair loss; the expression of collagen is increased, and the environment of hair follicles is changed, so that the beautiful appearance of the hairs is restored, and the hair follicles are healthier; the extract can also help maintain the hair growth cycle, optimize cell-to-cell and cell-to-matrix adhesion, increase protein expression, and promote hair growth and hair follicle renewal.

Drawings

Fig. 1 is a subcutaneous blood vessel map of the back skin of each group of mice on the 4 th day of the experiment on the influence of the essence of the 8% anti-hair-loss and hair-growth raw material of the invention on the hair growth cycle, wherein a is a positive control group, B is an experimental group, C is a matrix group, and D is a negative control group.

Fig. 2 shows the effect of the essence of 8% hair loss prevention and growth promotion raw material on the hair growth cycle at 12 th and 20 th days, and the back skin, paraffin sections and HE staining images of each group of mice are cut, wherein a is an experimental group, B is a positive control group, C is a matrix group, and D is a negative control group.

FIG. 3 is a graph showing the results of the in vitro assay of the present invention.

Detailed Description

In order to more clearly illustrate the present invention, specific examples are described below, which do not limit the scope of the present invention.

The raw material formula for preventing hair loss and growing hair is characterized by comprising the following components: ginger rhizome extract, ginkgo leaf extract, ginseng extract, coix seed extract and biota orientalis extract, ligusticum wallichii extract and zanthoxylum bungeanum fruit extract, tea leaf extract, gleditsia sinensis extract, angelica sinensis extract and oligopeptide-2, sage extract, menthol, ampelopsis japonica extract and saposhnikovia divaricata root extract, alcohol, hexanediol, caprylyl hydroximic acid/caprylic acid ester/propylene glycol mixture and p-hydroxyacetophenone.

The raw material formula for preventing alopecia and growing hair comprises the following components in parts by mass:

2-8% of ginger rhizome extract;

2-8% of ginkgo leaf extract;

2-8% of ginseng extract;

2-8% of coix seed extract;

7-13% of cacumen biotae extract;

2-8% of ligusticum wallichii extract;

2-8% of zanthoxylum bungeanum fruit extract;

2-8% of tea extract;

2-8% of gleditsia sinensis extract;

7-13% of angelica sinensis extract;

oligopeptide-25-11%;

2-8% of sage extract;

1-4% of menthol;

7-13% of ampelopsis japonica extract;

7-13% of radix sileris extract;

2-8% of alcohol;

0.1-0.3% of hexanediol;

0.5-0.7% of caprylhydroxamic acid/glyceryl caprylate/propylene glycol mixture;

0.1 to 0.3 percent of p-hydroxyacetophenone.

Example 1

Preferably, the raw material formula for preventing hair loss and growing hair consists of the following components in percentage by mass:

5% of ginger rhizome extract;

5% of ginkgo leaf extract;

5% of ginseng extract;

5% of coix seed extract;

10% of cacumen biotae extract;

5% of ligusticum wallichii extract;

5% of zanthoxylum bungeanum fruit extract;

5% of tea extract;

5% of gleditschia horrida extract;

10% of angelica extract;

oligopeptide-28%;

5% of sage extract;

1% of menthol;

10% of ampelopsis japonica extractive;

10% of radix sileris extract;

5% of alcohol;

0.2% of hexanediol;

0.6% caprylhydroxamic acid/glyceryl caprylate/propylene glycol mixture;

0.2 percent of p-hydroxyacetophenone.

Example 2

The raw material formula for preventing alopecia and growing hair comprises the following components in parts by mass:

2% of ginger rhizome extract;

8% of ginkgo leaf extract;

2% of ginseng extract;

8% of coix seed extract;

7% of cacumen biotae extract;

2% of ligusticum wallichii extract;

8% of zanthoxylum bungeanum fruit extract;

2% of tea extract;

8% of gleditsia sinensis extract;

13% of angelica extract;

oligopeptide-25%;

2% of sage extract;

4% of menthol;

7% of ampelopsis japonica extract;

13% of radix sileris extract;

8% of alcohol;

0.3% of hexanediol;

0.5% caprylhydroxamic acid/glyceryl caprylate/propylene glycol mixture;

0.2 percent of p-hydroxyacetophenone.

Example 3

The raw material formula for preventing alopecia and growing hair comprises the following components in parts by mass:

ginger rhizome extract 8%;

2% of ginkgo leaf extract;

8% of ginseng extract;

2% of coix seed extract;

13% of cacumen biotae extract;

8% of ligusticum wallichii extract;

2% of zanthoxylum bungeanum fruit extract;

8% of tea extract;

2% of gleditschia horrida extract;

13% of angelica extract;

oligopeptide-25%;

salvia officinalis extract 8%;

4% of menthol;

13% of ampelopsis japonica extract;

7% of radix sileris extract;

2% of alcohol;

0.2% of hexanediol;

0.5% caprylhydroxamic acid/glyceryl caprylate/propylene glycol mixture;

0.3 percent of p-hydroxyacetophenone.

The ginger rhizome extract, the ginkgo leaf extract, the ginseng extract, the coix seed extract, the Chinese arborvitae twig extract, the ligusticum wallichii extract, the zanthoxylum bungeanum fruit extract, the tea leaf extract, the gleditsia sinensis extract, the angelica sinensis extract, the oligopeptide-2, the sage extract, the ampelopsis japonica extract and the radix sileris extract are all water extracts.

The hair-loss preventing and hair-growing material consists of 19 kinds of material, including 15 active components.

The raw materials for preventing alopecia and growing hair are started from seven aspects according to the mechanism of alopecia:

promoting scalp blood circulation, and contains rhizoma Zingiberis recens (Zingiber OFFICINALE) root extract and folium Ginkgo (Ginkgo BILOBA) extract; they have the function of promoting scalp blood circulation;

the chemical components of ginger are complex, more than 100 are found, and the ginger can be classified into three categories of volatile oil, gingerol and diphenyl heptane. Gingerol is the main active substance of ginger, and has the biological activities of resisting oxidation, eliminating free radicals, inhibiting prostaglandin synthesis, resisting blood coagulation, inhibiting thrombosis, preventing and treating cardiovascular diseases, resisting tumor, resisting inflammation, resisting ulcer and the like.

Folium Ginkgo has effects of promoting blood circulation, relieving pain, removing blood stasis, dredging collaterals, astringing lung, and relieving asthma, and can be used for treating blood stasis. The components of ginkgo leaf include specific flavonoids, terpenes and ginkgolic acids which can cause allergic reactions. The flavonoids include more than 20 kinds of other flavonoids besides glycosides such as quercetin, kaempferol and isorhamnetin. Terpenes include bilobalide A, B, C and bilobalide, both of which have unique stereo structures not possessed by other plant terpenes. A large number of experimental research and clinical research data show that the ginkgo biloba extract can improve the blood circulation of the heart and the brain, expand the blood vessels of the heart and the brain, improve the blood rheology and eliminate free radicals, and has better curative effect on cardiovascular system diseases and nervous system diseases.

The components for improving hair follicle growth and development are Ginseng radix (PANAX GINSENG) extract, Coicis semen (Coix LACRYMA-JOBI) extract and Chinese arborvitae (Platysacus ORIENTALIS) leaf extract: ginseng contains complex chemical components such as ginsenoside, octaside, panaxadiol, triol, ginseng acid, ginsennin, essential oil, vitamins and glycoside. The ginsenoside has excellent bioactivity, and can promote cell metabolism, dilate capillary vessel, improve blood circulation, provide hair follicle tissue with reasonable nutrition, effectively improve scalp disease resistance, and protect and promote hair growth.

The Coicis semen extract has high protein, amino acids, vitamins and other active ingredients, and can improve activity of hair mother cell, and the extractive solution can be made into hair cosmetic for improving hair luster and promoting hair growth. Extract of leaves of biota ORIENTALIS (PLATYCLADUS ORIENTALIS): contains volatile oil mainly including fenchone, camphor, bornyl acetate and terpineol, and contains juninic acid quercetin, chrysin, kaempferide, hinokiflavone, wax, etc. The folium Platycladi contains volatile oil 0.26%, the main components of the oil are alpha-thujone, thujene, fenchone, etc., other components also contain flavonoids such as fructus Citri Junoris essence, quercetin, myricetin, hinokiflavone, etc., and the leaves also contain trace elements such as potassium, sodium, nitrogen, phosphorus, calcium, magnesium, manganese, zinc, etc. Has effects in cooling blood, stopping bleeding, promoting hair growth, and blackening hair.

Has antibacterial effect, and contains rhizoma Ligustici Chuanxiong (Ligusticum CHUANXIONG) extract and fructus Zanthoxyli (Zanthoxylum BUNGEANUM) extract.

Rhizoma Ligustici Chuanxiong (Ligusticum CHUANXIONG) extract has effects of promoting blood circulation and removing blood stasis, and is suitable for treating various diseases due to blood stasis, and the rhizome contains volatile oil about 1%. The oil is identified to have 40 components accounting for 93.64 percent of the volatile oil, wherein the main components are ligustilide accounting for 58 percent, 3-butylphthalide accounting for 5.29 percent and sabinene accounting for 6.08 percent, and the ligusticum wallichii has the inhibiting effect on large intestine, dysentery (Sonneratia), deformation, pyocyanea, typhoid fever, paratyphoid bacillus, vibrio cholerae and the like in vitro tests such as antibacterial effect. The rhizoma Ligustici Chuanxiong water infusion (1:3) also has inhibitory effect on some pathogenic dermatophytes in vitro.

Zanthoxylum BUNGEANUM (ZANTHOXYLUM BUNGEANUM) fruit extract: the chemical components of the pepper mainly comprise volatile oil, alkaloid, amide, coumarin, lignin, flavone, triterpene, sterol, hydrocarbon, fatty acid and the like, and the research on the pharmacological action mainly focuses on the 3 substances of the volatile oil, the alkaloid and the amide. The pepper has obvious inhibition effects on 10 gram positive bacteria such as anthrax, diphtheria, pneumococcus, hemolytic streptococcus, staphylococcus aureus, lemon and white staphylococcus and the like, and intestinal pathogenic bacteria such as large intestine, distortion, pyocyanea, typhoid fever, paratyphoid fever, vibrio cholerae and the like, has certain bacteriostasis and sterilization effects on 11 dermatophytes and 4 deep fungi, and is particularly sensitive to some deep fungi (such as microsporum lanosum, trichophyton rubrum and the like). Has stronger inhibiting and killing effect on mites of human bodies, and the inhibiting and killing effect on sebaceous Demodex mites is obviously stronger than that of follicular Demodex mites. The alpha-sanshool in the pepper has lethal toxicity to ascarid.

Cleaning, dandruff removing, and itching relieving effects, and the effective components are tea (CAMELLIA SINENSIS) extract and Gleditsia sinensis (GLEDITSIA SINENSIS) extract: tea (CAMELLIA SINENSIS) extract: has effects in cleaning hair, removing dandruff, relieving itching, regulating hair growth, and preventing alopecia, and phenolic hydroxyl group in tea extract has strong affinity and coagulation for keratin in hair and scalp. The tea leaf contains rich vitamins, mineral elements, amino acids and other biochemical components over 500, and the tea polyphenol and tannin in the tea extract can denature protein precipitate, so that it has the functions of resisting bacteria and diminishing inflammation. Meanwhile, tea polyphenol and other flavonoids have strong adsorption capacity. The tea saponin has the same properties as other plant saponin, such as surface activity and emulsification, and has no toxic and side effects, and is safe to use.

Gleditsia sinensis (GLEDITSIA SINENSIS) extract: the chemical components of the gleditsia sinensis are mainly terpenes, flavones, phenolic acids, steroids and the like, and terpenoids contained in the gleditsia sinensis plant are mainly pentacyclic triterpenes and glycoside compounds thereof, and the content of the terpenoids is 17% -20%. The total flavonoids of the honey locust spine have obvious inhibition effect on the release of Tumor Necrosis Factor (TNF) by macrophages in abdominal cavities of ICR mice, the inhibition effect on the TNF is enhanced along with the increase of the concentration of the total flavonoids of the honey locust spine, and the adjustment effect of the total flavonoids of the honey locust spine on cytokines is supposed to be the action mechanism of the anticancer activity of the honey locust spine. It has excellent anti-inflammatory activity, probably by a mechanism of reduced histamine release.

The hair growth period and rest period are prolonged, and the active ingredients are angelica (ANGELICA SINENSIS) extract and oligopeptide-2: the Angelica sinensis (ANGELICA SINENSIS) extract contains VVA, VB, palmitic acid, oleic acid, linoleic acid, myristic acid and beta-sitosterol. The active ingredients have effects of promoting blood circulation and cell growth, nourishing hair and skin, moistening hair, and preventing alopecia.

Oligopeptide-2: reactivation and re-concentration are carried out, 150 days, and 21, 480 new hairs grow out at most; hair loss was reduced by up to 76% after combing and washing;

reducing scalp oil secretion, and comprises extracts of Salvia officinalis (Salvia JAPONICA) and Mentholum: salvia officinalis (Salvia JAPONICA) extract, a chemical component of a plant of the genus Salvia, can be mainly divided into two types: phenolic compounds (phenolic aromatic acids, flavones, tannins, anthocyanins, and the like) and terpenes (monoterpenes, sesquiterpenes, diterpenes, and triterpenes). Other components include sterol, sugar, alkane, etc. The main components contained in the seeds include amino acids, fatty acids, lignans, and the like. Has good oil control activity, and the mechanism of the oil control activity is probably the efficacy exerted by active ingredients of flavonoid substances, tanshinone type phenanthrenequinone and rosinone type phenanthrenequinone.

Menthol: menthol is used as a main component, has special mint plant fragrance, has stimulation effect on cortex or mucosal receptors, and promotes the contraction of blood vessels so as to achieve the purpose of treatment; treating dandruff, excessive hair fat, etc.

Reducing sensitivity of male hormone, and comprises radix Ampelopsis (Ampelopsis JAPONICA) extract and radix Saposhnikoviae (Ledebouriella DIVARICATA) root extract: radix Ampelopsis (Ampelopsis JAPONICA) extract contains complex chemical components of radix Ampelopsis, and mainly contains various chemical components such as flavonoids, sterols, anthraquinones, phenolic acids and their glycosides, triterpenes, and lignans. The pharmacological action is as follows: has antibacterial, antitumor, exciting, immunity regulating, wound healing, granulation promoting, and ulcer healing effects. Can be applied to hair product for preventing alopecia caused by sexual hormone exuberance and promoting hair growth. Radix Saposhnikoviae (Ledebouriella DIVARICATA) root extract: the radix Saposhnikoviae contains small amount of volatile components, chromone component, coumarin component, polysaccharide component, organic acid component, glyceride, beta-sitosterol, daucosterol, D-mannitol, lignoceric acid, butadiene butyrate, adenosine, and trace elements Se, Mo, etc. Its pharmacological actions include antipyretic, analgesic, sedative, antiinflammatory, antitumor, immune function, antiallergic, antibacterial, antiviral, anticoagulant, hemostatic and anticonvulsant effects. Can be used in hair products for reducing androgen sensitivity and promoting hair growth.

The hair loss preventing and nourishing raw material of the invention can activate and nourish degenerated hair, re-compact the hair, recover and protect tenaciously, and refresh and beautify the hair quality and vitality

Experimental data:

in modern functional cosmetics, raw materials with specific efficacies are sometimes required to be added to achieve efficacy requirements in order to achieve a specific purpose or requirement. The hair loss preventing and hair nourishing material DepilationFair is added into the cosmetics to enable the cosmetics to meet the requirements required. The invention researches whether the anti-hair-loss and hair-nourishing raw material added into the cosmetics can achieve the expected effect to activate and nourish degenerated hair, re-compact the hair, recover and protect tenaciously and beautifully, and has beautiful hair quality and vitality.

Anti-drop effect

The method comprises the following steps:

establishing a mouse alopecia model: mixing rosin and paraffin wax 1:1, heating to melt, uniformly coating the mixture on the back of a mouse, wherein the coating area is about 2cm multiplied by 3cm, cooling to solidify, and removing the back hair. The anagen phase of the hair was confirmed to be in the telogen phase.

Animal grouping: the next day of depilation, selecting mice with smooth and undamaged depilation regions, randomly dividing the mice into 4 groups, counting 6 animals in each group, and respectively forming experimental groups: adding 8% of essence of hair loss preventing and hair growth promoting materials; positive control group: a serum of 5% minoxidil was administered; ③ group of matrices: the essence is given; fourthly, negative control group: no smearing of the product.

Smearing: the product is applied to local skin directly with cotton swab, 3 times daily, each time with a dose of 0.1ml, and continuously for 20 days.

And observing and recording results: observing the change of the skin and the hair color of the mice every day, and recording the time from smearing to blackening of the skin color of the hair-removed area of the back of each animal; the negative control group recorded the time from day 1 after depilation to the beginning of skin darkening in the depilated area; the hair removal zone begins to darken to full hair. On days 4, 12 and 20, skin tissue blocks at the same positions on the backs of the mice of each group are taken, fixed by 10% formalin, cut into sections embedded by paraffin, the sections are about 4-5 microns thick, stained by HE, and observed under a staining light microscope. The observation indexes are as follows: general histological examination: observing the skin integrity, whether reactions and degrees of congestion, edema, inflammatory cell infiltration and the like exist; ② counting hair follicles, counting hair follicles of 3 high power fields (x 400) per case, taking the mean value and performing statistical treatment.

Statistical analysis: the t-test of the mean of the two samples was performed using sps.11 statistical software.

Results

Effect of 8% essence of hair loss prevention and hair growth promoting material on hair growth cycle: firstly, the local skin of the experimental group of mice gradually appears black in 5-6 days after epilation, the hair enters the growth period, new hair grows out after 10 days, the skin color changes into pink (rest period) through gray (catagen) in 20 days, and the skin black and gray last for about 15 days; secondly, local skin of the positive control group of mice gradually becomes black on the 5 th day after epilation, hairs grow into a growth period, new hairs grow out after 9 days, the skin color is changed into pink (rest period) through gray (catagen) at 20 days, and the skin black and gray last for 15 days; ③ the mice in the substrate group and the negative control group are all changed from pink to black on the local skin 6 days after epilation, new hair grows out after 10 days, the skin color is changed from pink to pink through gray at 19 days, and the skin black and gray are continuously maintained for 13 days.

Histological examination:

on the 4 th day of the experiment, the back skin of each group of mice is cut, and the subcutaneous blood vessels of the experimental group and the positive control group are rich and thick; this was not observed in the matrix group and the negative control group, as shown in FIG. 1

On the 12 th and 20 th days of the experiment, the back skin of each group of mice is cut, and is sliced by paraffin, and observed by HE staining: the skin structure of each group of mice is complete, the covered squamous epithelium is complete, the cuticle layer is not thickened, and inflammatory cells are not infiltrated in the dermis. The hair follicles of the animal skin of the experimental group and the positive control group are developed more completely and more in quantity, and melanin is more obvious than that of the substrate group and the negative control group. There were no significant differences in the morphology of the hair follicles in each group, as shown in FIG. 2.

The effect of the essence of 8% hair loss prevention and hair growth promoting raw material on the density of hair follicles: the number of hair follicles in each group of mice on day 20 of the experiment is shown in table 1. The mice of the experimental group, the positive control group and the negative control group have very significant difference statistically, P is less than 0.001, and the mice of the matrix group have no significant difference compared with the negative control group, wherein P is more than 0.05. And compared with the positive control group, the experimental group has no significant difference, and P & gt is 0.05.

TABLE 1 Effect of Hair follicle count in groups of mice

Group of	Smearing essence	Number of follicles (number/high power field)
			Experimental group	Essence of 8% hair loss prevention and hair growth promotion raw material DepilationFair	19.17±2.12
Positive control group	Essence of 5% minoxidil	20.25±2.01
			Substrate group	Essence liquid	11.67±1.23
Negative control group	Non-smearing product	11.33±1.54

Mice are an animal model for studying the hair cycle, with periodic growth of hair, a meristematic, a catagen and a telogen phase. The hair of the mouse is black, melanocytes in the skin are uniformly distributed on hair follicles, the melanocytes are synthesized and secreted only in the hair growth period, and the melanocytes are transferred to keratinocytes, so that the skin is black; during the hair anaplastic phase, melanin synthesis is reduced and the skin gradually changes from black to grey; there is no melanin synthesis during telogen and the skin is pink. Histologically, the change in skin color in the mice reflected different periods of the hair growth cycle. When the mouse is bred in a good state for 6-8 weeks, all hair follicles enter a resting state (skin pink) from a growth phase (skin black), and do not automatically enter the growth phase under a physiological state, but can be induced to enter the growth phase after artificial hair removal, the skin color turns black after 5-6 days of hair removal, the black color turns grey again after 12 days of hair removal (a regression phase), and the skin turns pink after 2 days of hair removal (a resting state).

Minoxidil is a medicine for treating various alopecia and has been proved by research, and can make hair enter anagen in advance, prolong anagen period, shorten telogen period and enlarge hair follicle. Therefore, 5% minoxidil essence is selected as a positive control in the study.

The experiment finds that compared with a negative control group mouse, the skin color of the experimental group mouse is changed from pink to black in advance, 24H is changed from black to gray in delay, the hair follicle growth period time is about 72H longer than that of the negative control group, and the result is similar to that of the positive control group. The essence of 8 percent of the hair loss preventing and hair growing raw material DepilationFair is consistent with minoxidil, so that the hair follicle can be induced to enter a growth phase, the growth phase time is prolonged, and the hair follicle is delayed to enter a catagen phase. We also found that on day 4 of the experiment, the skin of the back of each group of mice was excised, and that the subcutaneous blood vessel network of the experimental group and the positive control group was abundant and the blood vessels were thick; this was not observed in the matrix group and the negative control group. It is presumed that the essence of hair loss preventing and hair growth promoting material depilationFair has the same effect of promoting the vascular proliferation as minoxidil. Meanwhile, on the 20 th day of the experiment, the numbers of the hair follicles of the mice of the experimental group, the positive control group and the negative control group are observed to have very significant difference statistically, and P is less than 0.001; the stroma group has no significant difference compared with the negative control group, and P & gt is 0.05; compared with the positive control group, the experimental group has no significant difference, and P & gt is 0.05. Therefore, the hair loss preventing and hair restoring material DepilationFair has the effect of promoting the growth of hair follicles, and the effect is equivalent to that of minoxidil.

Hair growth and restoration effects

In vitro testing:

the method comprises the following steps: 1 female volunteer of 45 years old smears essence of 8 percent of hair loss prevention and growth promotion raw material DepilationFair every day for three times every day for lasting use for 90 days, and the effect is shown in figure 3: the results show that: the hair is obviously strengthened and thickened, which shows that the hair loss preventing and growing raw material has the hair growing and repairing effect.

The research confirms that the hair loss preventing and hair growing raw material DepilationFair for the experiment consists of 16 active ingredients, and the functions and the efficacies of the active ingredients are also proved. According to the formula of the raw materials for preventing hair loss and nourishing hair, the raw materials adopt the plant extract, and the hair loss preventing and fixing effects of the plant extract are represented by increasing the expression of the hair hyalin and the keratin, reshaping the hair root structure, preventing scalp aging and minimizing hair loss; the expression of collagen is increased, and the environment of hair follicles is changed, so that the beautiful appearance of the hairs is restored, and the hair follicles are healthier; the extract can also help maintain the hair growth cycle, optimize cell-to-cell and cell-to-matrix adhesion, increase protein expression, and promote hair growth and hair follicle renewal.

The skilled person should understand that: although the invention has been described in terms of the above specific embodiments, the inventive concept is not limited thereto and any modification applying the inventive concept is intended to be included within the scope of the patent claims.

The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the invention may occur to those skilled in the art without departing from the principle of the invention, and are considered to be within the scope of the invention.

Claims (4)

1. The cosmetic composition for preventing alopecia and nourishing hair is characterized by comprising the following components in percentage by mass: 2-8% of ginger rhizome extract, 2-8% of ginkgo leaf extract, 2-8% of ginseng extract, 2-8% of coix seed extract, 7-13% of Chinese arborvitae leaf extract, 2-8% of ligusticum wallichii extract, 2-8% of zanthoxylum bungeanum fruit extract, 2-8% of tea leaf extract, 2-8% of gleditsia sinensis extract, 7-13% of Chinese angelica root extract, 25-11% of oligopeptide, 2-8% of salvia officinalis extract, 1-4% of menthol, 7-13% of ampelopsis japonica extract and 7-13% of saposhnikovia divaricata root extract, wherein the ginger rhizome extract, the ginkgo leaf extract, the ginseng extract, the coix seed extract, the Chinese arborvitae leaf extract, the ligusticum wallichii extract, the zanthoxylum bungeanum fruit extract, the tea leaf extract, the gleditsia sinensis extract, the Chinese angelica root extract, the salvia officinalis extract, the Chinese arborvitae extract, the Chinese magnoliaextract, the extract, the Chinese magnoliavine extract, the Chinese magnoliavine extract, the Chinese magnoliavine extract, the Chinese magnoliavine extract, the Chinese magnoliavine extract, the Chinese magnol, The ampelopsis japonica extract and the saposhnikovia divaricata root extract are both aqueous extracts.

2. The anti-hair loss and hair growth cosmetic composition as claimed in claim 1, further comprising alcohol, hexanediol, caprylhydroxamic acid/glyceryl caprylate/propylene glycol mixture, p-hydroxyacetophenone.

3. The anti-alopecia and hair-nourishing cosmetic composition according to claim 2,

the cosmetic composition comprises the following components in parts by mass:

2-8% of ginger rhizome extract;

2-8% of ginkgo leaf extract;

2-8% of ginseng extract;

2-8% of coix seed extract;

7-13% of cacumen biotae extract;

2-8% of ligusticum wallichii extract;

2-8% of zanthoxylum bungeanum fruit extract;

2-8% of tea extract;

2-8% of gleditsia sinensis extract;

7-13% of angelica sinensis extract;

oligopeptide-25-11%;

2-8% of sage extract;

1-4% of menthol;

7-13% of ampelopsis japonica extract;

7-13% of radix sileris extract;

2-8% of alcohol;

0.1-0.3% of hexanediol;

0.5-0.7% of caprylhydroxamic acid/glyceryl caprylate/propylene glycol mixture;

0.1 to 0.3 percent of p-hydroxyacetophenone.

4. The anti-alopecia and hair-nourishing cosmetic composition according to claim 3,

the cosmetic composition comprises the following components in parts by mass:

5% of ginger rhizome extract;

5% of ginkgo leaf extract;

5% of ginseng extract;

5% of coix seed extract;

10% of cacumen biotae extract;

5% of ligusticum wallichii extract;

5% of zanthoxylum bungeanum fruit extract;

5% of tea extract;

5% of gleditschia horrida extract;

10% of angelica extract;

oligopeptide-28%;

5% of sage extract;

1% of menthol;

10% of ampelopsis japonica extractive;

10% of radix sileris extract;

5% of alcohol;

0.2% of hexanediol;

0.6% caprylhydroxamic acid/glyceryl caprylate/propylene glycol mixture;

0.2 percent of p-hydroxyacetophenone.

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