TR201903889T4 - Korneal endotelyal hücreler üreten yönlendirilmiş diferansiyasyona yönelik yöntem. - Google Patents

Korneal endotelyal hücreler üreten yönlendirilmiş diferansiyasyona yönelik yöntem. Download PDF

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TR201903889T4
TR201903889T4 TR2019/03889T TR201903889T TR201903889T4 TR 201903889 T4 TR201903889 T4 TR 201903889T4 TR 2019/03889 T TR2019/03889 T TR 2019/03889T TR 201903889 T TR201903889 T TR 201903889T TR 201903889 T4 TR201903889 T4 TR 201903889T4
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endothelial cells
directed differentiation
corneal endothelial
cells
corneal
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TR2019/03889T
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English (en)
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L Mccabe Kathryn
Lu Shi-Jiang
Lanza Robert
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Astellas Inst For Regenerative Medicine
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Abstract

Bu tarifname genellikle, kornea bozuklukları dahil olmak üzere görsel bozuklukların tedavisine yönelik hücre esaslı terapiler ile ilgilidir. Yöntemler, korneal hücrelerin kök hücrelerden yönlendirilmiş diferansiyasyonu örneklendirmektedir. Korneal endotelyal hücrelerin bileşimleri ve bunların kullanımı da sağlanmaktadır. Örnek niteliğindeki bileşimler, iyileştirilmiş hücre yoğunluğu ve/veya bağışlanan dokudan elde edilen hücrelere göre daha "genç" gen ifadesini sergilemektedir.
TR2019/03889T 2011-12-06 2012-12-06 Korneal endotelyal hücreler üreten yönlendirilmiş diferansiyasyona yönelik yöntem. TR201903889T4 (tr)

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US201161567479P 2011-12-06 2011-12-06

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US (2) US9752118B2 (tr)
EP (2) EP2788472B1 (tr)
CA (2) CA2858173C (tr)
CY (1) CY1121383T1 (tr)
DK (1) DK2788472T3 (tr)
ES (1) ES2716200T3 (tr)
HR (1) HRP20190460T1 (tr)
HU (1) HUE041929T2 (tr)
LT (1) LT2788472T (tr)
PL (1) PL2788472T3 (tr)
PT (1) PT2788472T (tr)
RS (1) RS58526B1 (tr)
SI (1) SI2788472T1 (tr)
TR (1) TR201903889T4 (tr)
WO (1) WO2013086236A2 (tr)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10842669B2 (en) * 2008-11-13 2020-11-24 Gholam A. Peyman Ophthalmic drug delivery method
JP6519851B2 (ja) * 2012-07-06 2019-05-29 京都府公立大学法人 眼細胞の分化マーカーおよび分化制御
WO2014179716A2 (en) * 2013-05-03 2014-11-06 Emmetrope Ophthalmics Llc IDENTIFICATION AND ISOLATION OF HUMAN CORNEAL ENDOTHELIAL CELLS (HCECs)
WO2015016371A1 (ja) 2013-07-30 2015-02-05 京都府公立大学法人 角膜内皮細胞マーカー
US11730722B2 (en) 2013-07-30 2023-08-22 Kyoto Prefectural Public University Corporation Corneal endothelium ECM therapeutic medicaments
EP3031906A4 (en) * 2013-08-06 2017-01-25 Riken Method for producing anterior eye segment tissue
WO2015041606A1 (en) * 2013-09-17 2015-03-26 Singapore Health Services Pte Ltd Corneal stromal keratocyte culture
US11382904B2 (en) 2013-10-31 2022-07-12 Kyoto Prefectural Public University Corporation Therapeutic drug for diseases related to endoplasmic reticulum cell death in corneal endothelium
WO2015080297A1 (en) * 2013-11-27 2015-06-04 Kyoto Prefectural Public University Corporation Application of laminin to corneal endothelial cell culture
EP3213761B1 (en) 2014-10-31 2021-05-19 Kyoto Prefectural Public University Corporation Novel treatment of retina using laminin
JP6925805B2 (ja) * 2014-10-31 2021-08-25 京都府公立大学法人 ラミニンによる角膜の新規治療
IL303401A (en) 2015-03-23 2023-08-01 Astellas Inst For Regenerative Medicine Improved assays for the potential of retinal pigment epithelium (RPE) cells and photoreceptor progenitors
AU2016308818B2 (en) 2015-08-18 2022-04-21 Astellas Institute For Regenerative Medicine Clinical formulations
US11781110B2 (en) 2016-04-29 2023-10-10 The Regents Of The University Of California Induction of corneal endothelial cells
US20200032202A1 (en) * 2017-04-04 2020-01-30 The Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College A modified cell culture medium and uses thereof
US11433090B2 (en) 2017-06-16 2022-09-06 The Doshisha mTOR-inhibitor-containing medicine for treating or preventing ophthalmic symptoms, disorders, or diseases, and application thereof
US20200121652A1 (en) * 2017-06-16 2020-04-23 The Doshisha Compounds having caspase inhibitory activity, pharmaceutical agent containing said compounds and for treating or preventing corneal endothelial symptoms, disorders, or diseases, and application of said pharmaceutical agent
CN107475200B (zh) * 2017-08-08 2020-12-22 浙江大学 一种背根神经节源的神经嵴干细胞的分离、培养与分化方法
KR102010478B1 (ko) * 2017-11-01 2019-08-13 차의과학대학교 산학협력단 줄기 세포의 신경 능선 세포로의 분화용 조성물, 키트, 및 이를 이용한 방법
AU2018371437A1 (en) * 2017-11-24 2020-07-02 Sumitomo Chemical Company, Limited Production method for cell mass including neural cells/tissue and non-neural epithelial tissue, and cell mass from same
KR102114787B1 (ko) * 2018-04-02 2020-05-26 중앙대학교 산학협력단 리보핵산분해효소 5 고발현된 각막 내피세포를 포함하는 3차원 각막 내피 이식체 제조방법
JP7474520B2 (ja) * 2019-04-05 2024-04-25 ザ・ユニバーシティ・オブ・ホンコン 哺乳類の拡大ポテンシャル幹細胞のための培地、その組成物および方法
WO2020213969A2 (ko) * 2019-04-17 2020-10-22 연세대학교 산학협력단 각막 내피 세포의 기능 부전 진단용 바이오마커
AU2022270611A1 (en) 2021-05-03 2023-10-12 Astellas Institute For Regenerative Medicine Methods of generating mature corneal endothelial cells
CN113355424B (zh) * 2021-07-14 2022-03-25 江苏先声医学诊断有限公司 Pcdh11x突变在预测非小细胞肺癌患者对免疫检查点抑制剂疗法敏感性中的应用

Family Cites Families (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3421217A (en) 1967-06-29 1969-01-14 Schick Electric Inc Hair receptacle for electric shaver
US5166048A (en) 1981-03-02 1992-11-24 Soll David B Protection of human corneal endothelial cells
US4678783B1 (en) 1983-11-04 1995-04-04 Asahi Chemical Ind Substituted isoquinolinesulfonyl compounds
US4959319A (en) 1985-08-01 1990-09-25 Skelnik Debra L Process of corneal enhancement
US5269812A (en) 1986-05-16 1993-12-14 White Thomas C Methods and devices employed in replacement of the corneal endothelium
AU8532691A (en) 1990-08-01 1992-03-02 Chiron Ophthalmics Inc. Method for treating corneal endothelial wounds
US5589451A (en) 1992-09-21 1996-12-31 Board Of Regents, The University Of Texas System Methods and treaments for corneal healing with hepatocyte and keratinocyte growth factors
US5703047A (en) 1992-09-21 1997-12-30 Board Of Regents, The University Of Texas System Methods and treatments for corneal healing with growth factors
US5649944A (en) 1994-08-12 1997-07-22 Collins; Joseph Patrick Apparatus for preparing cornea material for tabbed (sutureless) transplantation
US5584881A (en) 1994-08-12 1996-12-17 Rowsey; J. James Sutureless corneal transplantation apparatus and method
US5755785A (en) 1994-08-12 1998-05-26 The University Of South Florida Sutureless corneal transplantation method
US5686414A (en) 1995-11-14 1997-11-11 Xoma Corporation Methods of treating conditions associated with corneal transplantation
JP2001508302A (ja) 1997-01-10 2001-06-26 ライフ テクノロジーズ，インコーポレイテッド 胚性幹細胞血清置換
WO1999020620A1 (fr) 1997-10-22 1999-04-29 Nippon Shinyaku Co Ltd Derive d'isoquinoleine et medicament
WO1999061403A1 (fr) 1998-05-25 1999-12-02 Santen Pharmaceutical Co., Ltd. Nouveaux derives vinylbenzene
US6541256B1 (en) 1999-02-11 2003-04-01 The Schepens Eye Research Institute, Inc. Growth medium for human corneal endothelial cells
US6548059B1 (en) 1999-07-22 2003-04-15 The Schepens Eye Research Institute, Inc. Promotion of proliferation of adult corneal endothelial cells
AUPR069500A0 (en) * 2000-10-11 2000-11-09 Coster, Douglas John Tissue modification
AU2002245709A1 (en) 2001-03-23 2002-10-08 Bayer Corporation Rho-kinase inhibitors
AR035791A1 (es) 2001-03-23 2004-07-14 Bayer Corp Compuesto n,n-diheterociclico de amina, inhibidor de la rho-quinasa, su uso para la fabricacion de un medicamento y proceso para la preparacion del compuesto
EP1403255A4 (en) 2001-06-12 2005-04-06 Sumitomo Pharma INHIBITORS OF RHO KINASE
AU2003202263A1 (en) 2002-01-10 2003-07-30 Bayer Healthcare Ag Roh-kinase inhibitors
US6943172B2 (en) 2002-01-23 2005-09-13 Bayer Pharmaceuticals Corporation Rho-kinase inhibitors
WO2003092762A1 (fr) * 2002-04-30 2003-11-13 Amniotec Inc. Feuille de type endothelium corneen et procede permettant de la produire
CN100354264C (zh) 2002-07-22 2007-12-12 旭化成制药株式会社 5－取代异喹啉衍生物及含有它们的药物
DE10237945A1 (de) 2002-08-20 2004-03-11 Quintis Gmbh Laserbasierte Vorrichtung zur nichtmechanischen, dreidimensionalen Trepanation bei Hornhauttransplantationen
CA2400996A1 (en) 2002-09-03 2004-03-03 Lisa Mckerracher 1,4-substituted cyclohexane derivatives
EP2230298A1 (en) 2003-02-06 2010-09-22 Cellseed Inc. Anterior ocular segment related cell sheets, three-dimensional structures, and processes for producing the same
EP1600177B1 (en) 2003-02-20 2016-05-25 Cellseed Inc. Endothelial cell sheet for cornea regeneration, method of producing the same and method of using the same
WO2004106378A2 (en) 2003-05-28 2004-12-09 Regeneron Pharmaceuticals, Inc. Method of treating corneal transplant rejection by using vegf antagonists
US7300654B2 (en) 2003-05-28 2007-11-27 Regeneron Pharmaceuticals, Inc. Method of treating corneal transplant rejection in high risk keratoplasty patients
US7160894B2 (en) 2003-06-06 2007-01-09 Asahi Kasei Pharma Corporation Tricyclic compound
JP2007516196A (ja) 2003-07-02 2007-06-21 ガラパゴス エヌブイ Ｒｈｏキナーゼ阻害剤としてのピラジン及びピラリジン誘導体
US7147648B2 (en) 2003-07-08 2006-12-12 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Device for cutting and holding a cornea during a transplant procedure
US20050182061A1 (en) 2003-10-02 2005-08-18 Jeremy Green Phthalimide compounds useful as protein kinase inhibitors
WO2005037197A2 (en) 2003-10-06 2005-04-28 Glaxo Group Limited Preperation of 1,6-disubstituted azabenzimidazoles as kinase inhibitors
EP1689393A4 (en) 2003-10-06 2008-12-17 Glaxo Group Ltd PREPARATION OF 1,7-DISUBSTITUTED AZABENZIMIDAZOLES AS KINASE INHIBITORS
EP1670466A4 (en) 2003-10-06 2007-04-25 Glaxo Group Ltd PREPARATION OF 1,6,7-TRISUBSTITUTED AZABENIC ZIMIDAZOLES AS KINASE-INHIBITORS
US20070275365A1 (en) 2003-10-10 2007-11-29 Lui Ge M Human Corneal Endothelial Cells and Methods of Obtaining and Culturing Cells for Corneal Cell Transplantation
JP2007509643A (ja) 2003-10-10 2007-04-19 ゲ・ミン・ルイ 角膜内皮および関連細胞のバイオポリマー上での増殖のための方法および組成物ならびに人工角膜移植片の作成
JP2007015928A (ja) 2003-10-15 2007-01-25 Ube Ind Ltd 新規オレフィン誘導体
JP4110324B2 (ja) 2003-10-15 2008-07-02 宇部興産株式会社 新規インダゾール誘導体
JP2007008816A (ja) 2003-10-15 2007-01-18 Ube Ind Ltd 新規イソキノリン誘導体
US7087369B2 (en) 2003-12-17 2006-08-08 The Regents Of The University Of California Cornea preservation medium
JP4834802B2 (ja) 2004-02-18 2011-12-14 聡 山上 ヒト角膜内皮細胞の培養物層積層体及びその作製方法
CN1980929A (zh) 2004-02-24 2007-06-13 比奥阿克松医疗技术股份有限公司 4－取代哌啶衍生物
US20060216821A1 (en) * 2004-02-26 2006-09-28 Reliance Life Sciences Pvt. Ltd. Pluripotent embryonic-like stem cells derived from corneal limbus, methods of isolation and uses thereof
KR20070002081A (ko) 2004-04-02 2007-01-04 버텍스 파마슈티칼스 인코포레이티드 Ｒｏｃｋ 및 기타 단백질 키나아제의 억제제로서 유용한아자인돌
JP2006006249A (ja) * 2004-06-28 2006-01-12 Hiroshima Univ 羊膜由来細胞の培養方法及びその利用
BRPI0513897A (pt) * 2004-07-29 2008-05-20 Stem Cell Innovations Inc diferenciação de células-tronco
JPWO2006057270A1 (ja) 2004-11-26 2008-06-05 旭化成ファーマ株式会社 含窒素３環化合物
EP1835023A4 (en) * 2004-12-09 2008-09-10 Amano Shiro PRECIPITATING CELL DERIVED FROM HUMAN HORNHAIL ENTHELIAL CELLS, OR DERIVED CELL ASSOCIATION, METHOD FOR THE MANUFACTURE THEREOF, AND METHOD FOR TRANSPLANTING THE PRECURSOR CELL OR THE CELL ASSOCIATION
US9999497B2 (en) 2005-01-31 2018-06-19 Yichieh Shiuey Corneal implants and methods and systems for placement
US7601487B2 (en) 2005-04-12 2009-10-13 Cleo Cosmetic And Pharmaceutical Co., Llc Composition using HMW dextran and method for in vitro preservation of corneal tissues
CN101253166B (zh) 2005-08-30 2013-07-10 旭化成制药株式会社 磺酰胺化合物
EP1945759A1 (en) 2005-10-12 2008-07-23 Cellular Bioengineering, Inc. Resorbable cornea button
US20070128722A1 (en) * 2005-12-05 2007-06-07 Industrial Technology Research Institute Human mesenchymal stem cells and culturing methods thereof
WO2007083685A1 (ja) 2006-01-19 2007-07-26 Senju Pharmaceutical Co., Ltd. 生体内で細胞増殖可能な角膜内皮製剤
TWI407967B (zh) 2006-04-28 2013-09-11 Ray Jui Fang Tsai 人類角膜內皮幹細胞之擴增方法
US20080050423A1 (en) 2006-08-23 2008-02-28 National Tsing Hua University Biopolymer-bioengineered cell sheet construct
JP5391488B2 (ja) * 2006-09-28 2014-01-15 ティシューテック・インコーポレイテッド 接着結合を持つ細胞の増殖を刺激するＲＮＡｉ方法および組成物
US20100022517A1 (en) * 2006-12-18 2010-01-28 Richards Lori A Ophthalmic formulation of rho kinase inhibitor compound
US8071779B2 (en) * 2006-12-18 2011-12-06 Inspire Pharmaceuticals, Inc. Cytoskeletal active rho kinase inhibitor compounds, composition and use
EP2177602B1 (en) * 2007-07-13 2013-09-11 Mitsubishi Tanabe Pharma Corporation Method for isolation of cell, serum-free culture medium for cell, and method for culture of cell
RU2473685C2 (ru) * 2007-07-31 2013-01-27 Лайфскен, Инк. Дифференцировка человеческих эмбриональных стволовых клеток
CA2697895C (en) 2007-08-29 2017-10-31 Senju Pharmaceutical Co., Ltd. Agent for promoting corneal endothelial cell adhesion
US20100297234A1 (en) * 2007-10-19 2010-11-25 Ilene Sugino Method of using an extracellular matrix to enhance cell transplant survival and differentiation
EP2209471A4 (en) 2007-10-30 2010-11-10 Univ Louisiana State PROTECTION OF LIPOXIN A4 FOR ENDOTHELIAL CORNEAL CELLS
WO2009132212A2 (en) 2008-04-25 2009-10-29 The Regents Of The University Of California Device to store and inject corneal graft
JP5305066B2 (ja) * 2008-05-09 2013-10-02 国立大学法人大阪大学 組織幹細胞／組織前駆細胞からの角膜内皮細胞の生成方法
US20100184033A1 (en) * 2008-07-16 2010-07-22 West Michael D Methods to accelerate the isolation of novel cell strains from pluripotent stem cells and cells obtained thereby
WO2008155748A2 (en) 2008-09-04 2008-12-24 Massimo Busin A new technique for preparing, storing and transplanting endothelial grafts
WO2010084970A1 (ja) * 2009-01-23 2010-07-29 国立大学法人大阪大学 標的細胞誘導用フィーダー細胞
US8642334B2 (en) * 2009-02-17 2014-02-04 Memorial Sloan Kettering Cancer Center Methods of neural conversion of human embryonic stem cells
WO2010099310A1 (en) 2009-02-26 2010-09-02 Wake Forest University Health Sciences Endothelial scaffolds
WO2010108126A2 (en) * 2009-03-19 2010-09-23 Fate Therapeutics, Inc. Reprogramming compositions and methods of using the same
EP2468312A4 (en) * 2009-08-19 2014-05-14 Univ Tohoku SHEET FOR KERATOPLASTIES
US20120219535A1 (en) * 2009-10-02 2012-08-30 University Of Southern California Cranial neural crest stem cells and culture condition that supports their growth
US20110143415A1 (en) * 2009-12-14 2011-06-16 Sergei Paylian Reprogramming normal and cancerous human cell lines into human induced poluripotent stem cells by co-electroporation with living xenopus laevis frog oocytes
WO2011080984A1 (en) * 2009-12-29 2011-07-07 Senju Pharmaceutical Co., Ltd. Therapeutic agent (y - 39983 ) for corneal endothelial dysfunction
WO2011096593A1 (ja) * 2010-02-05 2011-08-11 財団法人先端医療振興財団 角膜内皮細胞の培養方法、移植用角膜内皮細胞シートの製造方法および角膜内皮細胞培養キット
WO2011142364A1 (ja) * 2010-05-10 2011-11-17 独立行政法人理化学研究所 細胞シート作製方法
PL2577318T3 (pl) * 2010-05-25 2019-12-31 Memorial Sloan-Kettering Cancer Center Sposób różnicowania ludzkich embrionalnych komórek macierzystych do nocyceptorów i ich zastosowania
CA2806127C (en) 2010-07-23 2021-12-21 Advanced Cell Technology, Inc. Methods for detection of rare subpopulations of cells and highly purified compositions of cells
US20120028933A1 (en) * 2010-07-28 2012-02-02 Baust John M Cell Culture Media Supplement and Method of Molecular Stress Control
WO2012135621A2 (en) 2011-03-30 2012-10-04 Cellular Dynamics International. Inc Priming of pluripotent stem cells for neural differentiation
WO2013012087A1 (ja) * 2011-07-15 2013-01-24 国立大学法人大阪大学 角膜内皮細胞の調製方法
US9347042B2 (en) * 2011-10-06 2016-05-24 Keio University Method for producing corneal endothelial cell

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