WO2011056017A3 - 모낭 줄기세포의 대량 증식 방법 - Google Patents

모낭 줄기세포의 대량 증식 방법 Download PDF

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Publication number
WO2011056017A3
WO2011056017A3 PCT/KR2010/007794 KR2010007794W WO2011056017A3 WO 2011056017 A3 WO2011056017 A3 WO 2011056017A3 KR 2010007794 W KR2010007794 W KR 2010007794W WO 2011056017 A3 WO2011056017 A3 WO 2011056017A3
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WO
WIPO (PCT)
Prior art keywords
stem cells
hair follicle
follicle stem
culture medium
present
Prior art date
Application number
PCT/KR2010/007794
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English (en)
French (fr)
Other versions
WO2011056017A2 (ko
Inventor
라정찬
강성근
우상규
김미애
윤은지
Original Assignee
주식회사 알앤엘바이오
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 주식회사 알앤엘바이오 filed Critical 주식회사 알앤엘바이오
Priority to CN2010800598509A priority Critical patent/CN102712896A/zh
Priority to US13/505,127 priority patent/US20120269781A1/en
Publication of WO2011056017A2 publication Critical patent/WO2011056017A2/ko
Publication of WO2011056017A3 publication Critical patent/WO2011056017A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0627—Hair cells
    • C12N5/0628—Hair stem cells; Hair progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/14—Drugs for dermatological disorders for baldness or alopecia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes

Abstract

본 발명은 높은 수율로 모낭 줄기세포를 증식시키는 방법에 관한 것으로, 더욱 자세하게는 모낭 줄기세포 배양시 특정 농도의 ROCK 저해제(Rho-associated kinase inhibitor)를 첨가한 특정 배지를 사용하여 대량으로 모낭 줄기세포를 수득하는 방법 및 이에 사용되는 배지에 관한 것이다. 본 발명에 따른 모낭 줄기세포 배양용 배지는 두피 조직으로부터 분리된 모낭 줄기세포를 임상에 적용할 수 있을 정도의 양으로 증식 시킬 수 있어, 상기 모낭 줄기세포를 탈모증 및 무모증 등을 위한 발모용 세포치료제로 유용하게 사용될 수 있다.
PCT/KR2010/007794 2009-11-06 2010-11-05 모낭 줄기세포의 대량 증식 방법 WO2011056017A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN2010800598509A CN102712896A (zh) 2009-11-06 2010-11-05 用于增殖毛囊干细胞的方法
US13/505,127 US20120269781A1 (en) 2009-11-06 2010-11-05 Method for proliferating hair follicle stem cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2009-0107184 2009-11-06
KR20090107184 2009-11-06

Publications (2)

Publication Number Publication Date
WO2011056017A2 WO2011056017A2 (ko) 2011-05-12
WO2011056017A3 true WO2011056017A3 (ko) 2011-11-03

Family

ID=43970568

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2010/007794 WO2011056017A2 (ko) 2009-11-06 2010-11-05 모낭 줄기세포의 대량 증식 방법

Country Status (4)

Country Link
US (1) US20120269781A1 (ko)
KR (1) KR101255649B1 (ko)
CN (1) CN102712896A (ko)
WO (1) WO2011056017A2 (ko)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5885233B2 (ja) * 2011-06-01 2016-03-15 重昭 石坂 毛包幹細胞の培養方法
US9655930B2 (en) 2012-10-01 2017-05-23 Aderans Research Institute, Inc. Compositions and methods for producing reconstituted skin
NL2010222C2 (en) * 2013-02-01 2014-08-04 Conradus Ghosal Gho Composition and method for generating a desired cell type and/or tissue type from hair follicular stem cells.
FR3041656A1 (fr) * 2015-09-29 2017-03-31 Oreal Utilisation des cellules de la matrice pour la preparation d'un microfollicule pileux
KR101658333B1 (ko) * 2016-03-31 2016-09-20 주식회사 뮤제코스메틱 Bca 복합 발효물 및 모낭줄기세포 추출물을 함유하는 탈모방지 및 모발성장 조성물
FR3061207B1 (fr) * 2016-12-23 2023-03-10 Oreal Utilisation des cellules germinales pour la preparation d’un microfollicule pileux
EP3567096A4 (en) * 2017-01-06 2020-10-21 Olympus Corporation CELL OBSERVATION SYSTEM
CN106854640A (zh) * 2017-01-16 2017-06-16 广东万海细胞生物科技有限公司 一种毛囊干细胞的无血清培养基及其制备方法
CN106635967A (zh) * 2017-01-16 2017-05-10 广东万海细胞生物科技有限公司 一种毛囊干细胞的移植方法
CN110484490A (zh) * 2019-07-31 2019-11-22 杭州荣泽生物科技有限公司 一种毛囊干细胞的移植方法
WO2021162206A1 (ko) * 2020-02-10 2021-08-19 주식회사 마이크로바이오틱스 모낭조직 배양액을 포함하는 탈모의 예방 또는 치료용 조성물
CN114317404A (zh) * 2021-12-17 2022-04-12 上海纳米技术及应用国家工程研究中心有限公司 适用于毛囊干细胞体外培养的培养基配方和用于体外3d毛囊干细胞的培养方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100771171B1 (ko) * 2006-07-05 2007-10-29 재단법인서울대학교산학협력재단 모낭 줄기세포의 분리, 증식 및 분화 방법 및 대머리치료용 조성물
KR20080097593A (ko) * 2007-05-02 2008-11-06 라정찬 인간 지방조직 유래 다분화능 줄기세포 및 모낭세포를함유하는 세포치료제

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6824973B2 (en) * 2000-02-03 2004-11-30 Kirin Beer Kabushiki Kaisha Method of promoting stem cell proliferation or survival by contacting a cell with a stem cell factor-like polypeptide
US20080268054A1 (en) * 2000-12-04 2008-10-30 Eugene Bell Dermal derived human stem cells and compositions and methods thereof
DE10224982A1 (de) * 2002-06-05 2003-12-24 Rolf Hoffmann Mesenchymale Stammzellen des Haarfollikels und deren Verwendung
US20050042595A1 (en) * 2003-08-14 2005-02-24 Martin Haas Banking of multipotent amniotic fetal stem cells
WO2008002662A2 (en) * 2006-06-28 2008-01-03 The Univeristy Of Kansas Differentiation of stem cells from umbilical cord matrix into hepatocyte lineage cells
JP5419279B2 (ja) * 2007-01-17 2014-02-19 ウィスコンシン アラムニ リサーチ ファンデーション 改良された幹細胞の培養

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100771171B1 (ko) * 2006-07-05 2007-10-29 재단법인서울대학교산학협력재단 모낭 줄기세포의 분리, 증식 및 분화 방법 및 대머리치료용 조성물
KR20080097593A (ko) * 2007-05-02 2008-11-06 라정찬 인간 지방조직 유래 다분화능 줄기세포 및 모낭세포를함유하는 세포치료제

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WATANABE, K. ET AL.: "A ROCK inhibitor permits survival of dissociated human embryonic stem cells", NATURE BIOTECHNOLOGY, vol. 25, no. 6, 27 May 2007 (2007-05-27), pages 681 - 686, XP002478043, DOI: doi:10.1038/nbt1310 *

Also Published As

Publication number Publication date
CN102712896A (zh) 2012-10-03
US20120269781A1 (en) 2012-10-25
KR101255649B1 (ko) 2013-04-16
WO2011056017A2 (ko) 2011-05-12
KR20110050377A (ko) 2011-05-13

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