FR3061207B1 - Utilisation des cellules germinales pour la preparation d’un microfollicule pileux - Google Patents

Utilisation des cellules germinales pour la preparation d’un microfollicule pileux Download PDF

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Publication number
FR3061207B1
FR3061207B1 FR1663324A FR1663324A FR3061207B1 FR 3061207 B1 FR3061207 B1 FR 3061207B1 FR 1663324 A FR1663324 A FR 1663324A FR 1663324 A FR1663324 A FR 1663324A FR 3061207 B1 FR3061207 B1 FR 3061207B1
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FR
France
Prior art keywords
preparation
germ cells
microfollicle
hair
hair microfollicle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1663324A
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English (en)
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FR3061207A1 (fr
Inventor
Theebah Sellathurai
Khalid Bakkar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LOreal SA
Original Assignee
LOreal SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR1663324A priority Critical patent/FR3061207B1/fr
Application filed by LOreal SA filed Critical LOreal SA
Priority to KR1020197017361A priority patent/KR102573452B1/ko
Priority to ES17816746T priority patent/ES2959972T3/es
Priority to KR1020217024611A priority patent/KR20210100206A/ko
Priority to CN201780078701.9A priority patent/CN110088271A/zh
Priority to PCT/EP2017/082548 priority patent/WO2018114504A1/fr
Priority to JP2019533392A priority patent/JP6921960B2/ja
Priority to EP17816746.6A priority patent/EP3559211B1/fr
Priority to US16/462,346 priority patent/US20200181571A1/en
Publication of FR3061207A1 publication Critical patent/FR3061207A1/fr
Application granted granted Critical
Publication of FR3061207B1 publication Critical patent/FR3061207B1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0627—Hair cells
    • C12N5/0628—Hair stem cells; Hair progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/14—Drugs for dermatological disorders for baldness or alopecia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0666—Mesenchymal stem cells from hair follicles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/092—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells hair cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1352—Mesenchymal stem cells
    • C12N2502/1376—Mesenchymal stem cells from hair follicles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Rheumatology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Cosmetics (AREA)
FR1663324A 2016-12-23 2016-12-23 Utilisation des cellules germinales pour la preparation d’un microfollicule pileux Active FR3061207B1 (fr)

Priority Applications (9)

Application Number Priority Date Filing Date Title
FR1663324A FR3061207B1 (fr) 2016-12-23 2016-12-23 Utilisation des cellules germinales pour la preparation d’un microfollicule pileux
ES17816746T ES2959972T3 (es) 2016-12-23 2017-12-13 Uso de células germinales para preparar un microfolículo piloso
KR1020217024611A KR20210100206A (ko) 2016-12-23 2017-12-13 미세 모낭의 제조에 있어서의 배 세포의 용도
CN201780078701.9A CN110088271A (zh) 2016-12-23 2017-12-13 芽细胞用于制备微毛囊的用途
KR1020197017361A KR102573452B1 (ko) 2016-12-23 2017-12-13 미세 모낭의 제조에 있어서의 배 세포의 용도
PCT/EP2017/082548 WO2018114504A1 (fr) 2016-12-23 2017-12-13 Utilisation de cellules germinales pour la préparation d'un micro-follicule pileux
JP2019533392A JP6921960B2 (ja) 2016-12-23 2017-12-13 微小毛包を調製するための胚細胞の使用
EP17816746.6A EP3559211B1 (fr) 2016-12-23 2017-12-13 Utilisation de cellules germinales pour la préparation d'un micro-follicule pileux
US16/462,346 US20200181571A1 (en) 2016-12-23 2017-12-13 Use of germ cells for preparing a micro hair follicle

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1663324 2016-12-23
FR1663324A FR3061207B1 (fr) 2016-12-23 2016-12-23 Utilisation des cellules germinales pour la preparation d’un microfollicule pileux

Publications (2)

Publication Number Publication Date
FR3061207A1 FR3061207A1 (fr) 2018-06-29
FR3061207B1 true FR3061207B1 (fr) 2023-03-10

Family

ID=59070717

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1663324A Active FR3061207B1 (fr) 2016-12-23 2016-12-23 Utilisation des cellules germinales pour la preparation d’un microfollicule pileux

Country Status (8)

Country Link
US (1) US20200181571A1 (fr)
EP (1) EP3559211B1 (fr)
JP (1) JP6921960B2 (fr)
KR (2) KR102573452B1 (fr)
CN (1) CN110088271A (fr)
ES (1) ES2959972T3 (fr)
FR (1) FR3061207B1 (fr)
WO (1) WO2018114504A1 (fr)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5556783A (en) * 1991-03-27 1996-09-17 Trustees Of Univ. Of Penna Methods of culturing and modulating the growth of hair follicular stem cells
GB0605450D0 (en) * 2006-03-17 2006-04-26 Intercytex Ltd Cell co-culture
CN101479376A (zh) * 2006-06-27 2009-07-08 株式会社资生堂 含有源自身体的多个细胞种类的、能形成原始的器官样的细胞块
FR2903702B1 (fr) * 2006-07-13 2012-10-19 Oreal Equivalent d'epiderme capable de se pigmenter obtenu a partir de cellules de la matrice, procede de preparation et utilisation
EP2105499A1 (fr) 2008-03-28 2009-09-30 Technische Universität Berlin Procédés de production de nouvelles papilles et microfollicules capillaires et leur utilisation dans des tests in vitro et implantations in vivo
CN102712896A (zh) * 2009-11-06 2012-10-03 Rnl生物技术株式会社 用于增殖毛囊干细胞的方法
US9321998B2 (en) * 2011-02-24 2016-04-26 Organ Technologies Inc. Method of preparing regenerated hair follicle germ for transplantation in which hair color is controlled, composition including regenerated hair follicle germ for transplantation, and method of transplanting regenerated hair follicle germ
JP5885233B2 (ja) * 2011-06-01 2016-03-15 重昭 石坂 毛包幹細胞の培養方法
JP6218238B2 (ja) * 2014-07-10 2017-10-25 国立大学法人大阪大学 人工皮膚及びその製造方法

Also Published As

Publication number Publication date
EP3559211C0 (fr) 2023-07-19
KR102573452B1 (ko) 2023-08-31
JP6921960B2 (ja) 2021-08-18
EP3559211B1 (fr) 2023-07-19
FR3061207A1 (fr) 2018-06-29
JP2020501578A (ja) 2020-01-23
KR20190080939A (ko) 2019-07-08
ES2959972T3 (es) 2024-02-29
KR20210100206A (ko) 2021-08-13
EP3559211A1 (fr) 2019-10-30
US20200181571A1 (en) 2020-06-11
WO2018114504A1 (fr) 2018-06-28
CN110088271A (zh) 2019-08-02

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