SK286095B6 - Spôsob súčasného testovania množstva vzoriek rôznych látok - Google Patents
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| PCT/US1998/026465 WO1999030154A2 (en) | 1997-12-12 | 1998-12-11 | Continuous format high throughput screening |
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| SK8362000A3 SK8362000A3 (en) | 2000-10-09 |
| SK286095B6 true SK286095B6 (sk) | 2008-03-05 |
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| SK836-2000A SK286095B6 (sk) | 1997-12-12 | 1998-12-11 | Spôsob súčasného testovania množstva vzoriek rôznych látok |
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| PL186473B1 (pl) | 1996-01-26 | 2004-01-30 | Virco Nv | Sposób oceny in vitro chemioterapii HIV u pacjentów HIV dodatnich oraz sposób oznaczania fenotypowej wrażliwości lekowej poszczególnych szczepów HIV u pacjenta na inhibitory co najmniej dwóch enzymówkodowanych przez gen pol HIV |
| US6790652B1 (en) * | 1998-01-08 | 2004-09-14 | Bioimage A/S | Method and apparatus for high density format screening for bioactive molecules |
| US6410332B1 (en) | 1998-09-08 | 2002-06-25 | Symyx Technologies, Inc. | Sampling and analysis of reactions by trapping reaction components on a sorbent |
| CN1315001A (zh) * | 1998-09-08 | 2001-09-26 | 泰博特克有限公司 | 快速筛选分析物的方法 |
| AU2368900A (en) * | 1998-12-18 | 2000-07-03 | Symyx Technologies, Inc. | Apparatus and method for characterizing libraries of different materials using x-ray scattering |
| US20040185432A1 (en) * | 1999-01-08 | 2004-09-23 | Bioimage A/S | Method and apparatus for high density format screening for bioactive molecules |
| US6690399B1 (en) * | 1999-05-07 | 2004-02-10 | Tropix, Inc. | Data display software for displaying assay results |
| US6800437B1 (en) | 1999-08-06 | 2004-10-05 | Tibotec Bvba | Method of monitoring a biological system by labeling with an apo metal binding protein |
| US6544480B1 (en) | 1999-10-26 | 2003-04-08 | Tibotec Bvba | Device and related method for dispensing small volumes of liquid |
| US20010044112A1 (en) * | 2000-03-24 | 2001-11-22 | Lyles Mark B. | High throughput screening array containing porous material |
| US20030031675A1 (en) | 2000-06-06 | 2003-02-13 | Mikesell Glen E. | B7-related nucleic acids and polypeptides useful for immunomodulation |
| WO2001096597A2 (en) * | 2000-06-13 | 2001-12-20 | Glaxo Group Limited | High throughput screening of compounds for biological activity |
| US7087375B1 (en) | 2000-06-23 | 2006-08-08 | Johan Lennerstrand | Method for determining the mechanism of HIV RT inhibitors |
| US7052659B1 (en) | 2000-07-18 | 2006-05-30 | General Electric Company | Sequential high throughput screening method and system |
| US6653124B1 (en) | 2000-11-10 | 2003-11-25 | Cytoplex Biosciences Inc. | Array-based microenvironment for cell culturing, cell monitoring and drug-target validation |
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| US20030060695A1 (en) * | 2001-03-07 | 2003-03-27 | Connelly Patrick R. | Implantable artificial organ devices |
| US7244232B2 (en) * | 2001-03-07 | 2007-07-17 | Biomed Solutions, Llc | Process for identifying cancerous and/or metastatic cells of a living organism |
| US20020142304A1 (en) * | 2001-03-09 | 2002-10-03 | Anderson Daniel G. | Uses and methods of making microarrays of polymeric biomaterials |
| US20080009019A1 (en) * | 2001-06-13 | 2008-01-10 | Smithkline Beecham Corporation | High throughput method for screening candidate compounds for biological activity |
| DE10138766A1 (de) * | 2001-08-07 | 2003-02-20 | Bayer Cropscience Ag | Verfahren zum Identifizieren von makrocyclischen Polyketiden |
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| EP1298433A1 (en) * | 2001-09-28 | 2003-04-02 | EMC microcollections GmbH | Device and method for the generation of arrays of substance mixtures by diffusion |
| JP2005511066A (ja) * | 2001-12-07 | 2005-04-28 | ダイアックス、コープ | 磁気応答性粒子を洗浄する方法及び装置 |
| EP1463807A4 (en) * | 2001-12-19 | 2006-04-12 | Bristol Myers Squibb Co | FORMATHYDROGENASE FROM PICHIA PASTORIS AND USES THEREOF |
| US20050054118A1 (en) * | 2002-02-27 | 2005-03-10 | Lebrun Stewart J. | High throughput screening method |
| AU2003212471A1 (en) * | 2002-02-27 | 2003-09-09 | Miragene, Inc. | Improved substrate chemistry for protein immobilization on a rigid support |
| US20040072274A1 (en) * | 2002-05-09 | 2004-04-15 | Lebrun Stewart J. | System and method for visualization and digital analysis of protein and other macromolecule microarrays |
| EP1534755B1 (en) | 2002-05-10 | 2011-10-12 | Bio-Layer Pty Limited | Generation of surface coating diversity |
| EP1900827A3 (en) | 2002-05-21 | 2008-04-16 | Bayer HealthCare AG | Methods and compositions for the prediction, diagnosis, prognosis, prevention and treatment of malignant neoplasia |
| US20040028804A1 (en) * | 2002-08-07 | 2004-02-12 | Anderson Daniel G. | Production of polymeric microarrays |
| USD480815S1 (en) | 2002-08-13 | 2003-10-14 | Discovery Partners International | Card carrying an array of compounds |
| AU2003255253A1 (en) * | 2002-08-13 | 2004-02-25 | Discovery Partners International | Spotting pattern for placement of compounds in an array |
| US20040032058A1 (en) * | 2002-08-13 | 2004-02-19 | Neeper Robert K. | Systems and methods for casting and handling assay matrices |
| US8557743B2 (en) * | 2002-09-05 | 2013-10-15 | Dyax Corp. | Display library process |
| US20040058327A1 (en) * | 2002-09-20 | 2004-03-25 | Pan Jeffrey Y | Method for using a blank matrix in a continuous format high throughput screening process |
| US20050008212A1 (en) * | 2003-04-09 | 2005-01-13 | Ewing William R. | Spot finding algorithm using image recognition software |
| US7025935B2 (en) * | 2003-04-11 | 2006-04-11 | Illumina, Inc. | Apparatus and methods for reformatting liquid samples |
| WO2004106934A2 (en) * | 2003-05-28 | 2004-12-09 | Bayer Healthcare Ag | Diagnostics and therapeutics for diseases associated with 5-hydroxytryptamine (serotonin) receptor 7 (5-ht7) |
| JP2005046121A (ja) * | 2003-07-31 | 2005-02-24 | Japan Science & Technology Agency | オンチップバイオアッセイ方法及びキット |
| US20050136536A1 (en) * | 2003-09-15 | 2005-06-23 | Anderson Daniel G. | Embryonic epithelial cells |
| US20050069462A1 (en) * | 2003-09-30 | 2005-03-31 | International Business Machines Corporation | Microfluidics Packaging |
| US20050069949A1 (en) * | 2003-09-30 | 2005-03-31 | International Business Machines Corporation | Microfabricated Fluidic Structures |
| EP1892306A3 (en) | 2003-10-06 | 2008-06-11 | Bayer HealthCare AG | Methods and kits for investigating cancer |
| US20060127963A1 (en) * | 2003-11-21 | 2006-06-15 | Lebrun Stewart J | Microarray-based analysis of rheumatoid arthritis markers |
| JP2007516704A (ja) | 2003-12-02 | 2007-06-28 | ビーエーエスエフ アクチェンゲゼルシャフト | 除草剤の標的としての２−メチル−６−ソラニルベンゾキノン・メチルトランスフェラーゼ |
| US20050124017A1 (en) * | 2003-12-05 | 2005-06-09 | Stewart Lebrun | Quantitative alkaline-phosphatase precipitation reagent and methods for visualization of protein microarrays |
| AU2004296412B2 (en) * | 2003-12-12 | 2011-03-10 | Anteo Technologies Pty Ltd | A method for designing surfaces |
| CA2553468A1 (en) * | 2004-01-21 | 2005-08-04 | Istituto Di Ricerche Di Biologia Molecolare P. Angeletti Spa | Inhibitors of mammalian hdac 11 useful for treating hdac 11 mediated disorders |
| EP1564556A1 (de) * | 2004-02-17 | 2005-08-17 | DST Diagnostic Science & Technology GmbH | Verfahren und Vorrichtung zur Bestimmung mehrerer Analyten mit simultaner interner Kontrolle |
| US8101431B2 (en) | 2004-02-27 | 2012-01-24 | Board Of Regents, The University Of Texas System | Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems |
| US8105849B2 (en) | 2004-02-27 | 2012-01-31 | Board Of Regents, The University Of Texas System | Integration of fluids and reagents into self-contained cartridges containing sensor elements |
| EP1773866B1 (en) | 2004-07-02 | 2013-06-19 | Bio-Layer Pty Limited | Use of metal complexes |
| US20080206855A1 (en) * | 2005-05-06 | 2008-08-28 | Kalypsys, Inc. | Automated Compound Screening Using Gel-Permeation Matrix and Pin-Based Sample Transfer |
| US7629115B2 (en) * | 2005-05-13 | 2009-12-08 | Honeywell International Inc. | Cell-based platform for high throughput screening |
| AU2006309284B2 (en) | 2005-05-31 | 2012-08-02 | Board Of Regents, The University Of Texas System | Methods and compositions related to determination and use of white blood cell counts |
| ES2389111T3 (es) | 2005-12-02 | 2012-10-23 | Sirtris Pharmaceuticals, Inc. | Ensayos de espectrometría de masas para la actividad acetiltransferasa/desacetilasa |
| WO2007076580A1 (en) * | 2005-12-30 | 2007-07-12 | Bio-Layer Pty Limited | Binding of molecules |
| US8715665B2 (en) | 2007-04-13 | 2014-05-06 | The General Hospital Corporation | Methods for treating cancer resistant to ErbB therapeutics |
| US20090118135A1 (en) * | 2007-06-08 | 2009-05-07 | The Burnham Institute | Methods and compounds for regulating apoptosis |
| US20090272657A1 (en) * | 2008-05-01 | 2009-11-05 | Massachusetts Institute Of Technology | Devices and processes for analyzing nucleic acid damage and repair using electrophoresis |
| US9594054B2 (en) * | 2012-07-25 | 2017-03-14 | Bio-Rad Laboratories, Inc. | Targeted delivery of reagents to spots on a planar support through patterned transfer sheets |
| US9447135B2 (en) | 2012-12-14 | 2016-09-20 | University Of Kentucky Research Foundation | Semi-synthetic mithramycin derivatives with anti-cancer activity |
| GB201318814D0 (en) | 2013-10-24 | 2013-12-11 | Petroleum & Chemical Corp | Microfluidic devices and arrangements for supplying such devices with reagents and biological samples |
| EP3087391B1 (en) | 2013-12-23 | 2021-05-19 | Basf Se | Methods for determining modulators of insect transient receptor potential v (trpv) channel |
| WO2019023559A1 (en) | 2017-07-27 | 2019-01-31 | University Of Southern California | OTOPETRIN PROTON CHANNELS AS TARGETS FOR PHARMACEUTICAL DISCOVERY |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4447526A (en) * | 1981-04-20 | 1984-05-08 | Miles Laboratories, Inc. | Homogeneous specific binding assay with carrier matrix incorporating specific binding partner |
| EP0063810B1 (en) * | 1981-04-29 | 1986-03-05 | Ciba-Geigy Ag | New devices and kits for immunological analysis |
| US4591570A (en) * | 1983-02-02 | 1986-05-27 | Centocor, Inc. | Matrix of antibody-coated spots for determination of antigens |
| JP2564181B2 (ja) * | 1988-11-24 | 1996-12-18 | コニカ株式会社 | 特定成分の分析方法 |
| DE4208645A1 (de) * | 1992-03-18 | 1993-09-23 | Bayer Ag | Optischer festphasenbiosensor auf basis fluoreszenzfarbstoffmarkierter polyionischer schichten |
| WO1994002515A1 (en) * | 1992-07-21 | 1994-02-03 | Bunsen Rush Laboratories Inc. | Oligomer library formats and methods relating thereto |
| CA2130947C (en) * | 1993-11-12 | 2001-02-06 | Robert E. Emmons | Dry elements, test devices, test kits and methods for chemiluminescent detection of analytes using peroxidase-labeled reagents |
| EP0758313A4 (en) * | 1994-05-06 | 1999-09-15 | Pharmacopeia Inc | COMBINATORIAL LIBRARY OF DIHYDROBENZOPYRANES |
| US5856083A (en) * | 1994-05-06 | 1999-01-05 | Pharmacopeia, Inc. | Lawn assay for compounds that affect enzyme activity or bind to target molecules |
| AU2986395A (en) * | 1994-07-22 | 1996-02-22 | Zeneca Limited | Method for screening compound libraries |
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