SI2367940T1 - Mutirane penicilin G acilaze - Google Patents
Mutirane penicilin G acilaze Download PDFInfo
- Publication number
- SI2367940T1 SI2367940T1 SI200931814T SI200931814T SI2367940T1 SI 2367940 T1 SI2367940 T1 SI 2367940T1 SI 200931814 T SI200931814 T SI 200931814T SI 200931814 T SI200931814 T SI 200931814T SI 2367940 T1 SI2367940 T1 SI 2367940T1
- Authority
- SI
- Slovenia
- Prior art keywords
- acylase
- penicillin
- mutated
- apa
- semi
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
- C12N9/84—Penicillin amidase (3.5.1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
- C12P35/04—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by acylation of the substituent in the 7 position
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
- C12P37/04—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin by acylation of the substituent in the 6 position
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
- C12Y305/01011—Penicillin amidase (3.5.1.11), i.e. penicillin-amidohydrolase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Claims (12)
- Mutirane penicilin G acilaze Patentni zahtevki1. Mutirana prokariontska penicilin G acilaza,ki ima zaporedje SEQ ID No: 1 ali ima zaporedje, ki ima od 80% do 100% enakost z zaporedjem SEQ ID No: 1, pridobljenim iz divjega tipa penicilin G acilaze, značilna po tem, da ima mutant alanin na položaju B24 in ima levcin na položaju B460, v skladu s številčenjem amino kislin Escherichia coli penicilin G acilaze, ki ima zaporedje prikazano v SEQ ID No: 1.
- 2. Mutirana prokariontska penicilin G acilaza po zahtevku 1, ki ima levcin na položaju A3 ali ima lizin na položaju A90 ali ima glutaminsko kislino na položaju A192 in/ali ima levcin na položaju B148.
- 3. Nukleinska kislina, ki kodira mutirane penicilin G acilazo po kateremkoli predhodnem zahtevku.
- 4. Ekspresijski vektor, ki vsebuje nukleinsko kislino po zahtevku 3.
- 5. Gostiteljska celica, ki vsebuje ekspresijski vektor po zahtevku 4.
- 6. Postopek izdelave mutirane penicilin G acilaze, po kateremkoli zahtevku od 1 do 2.
- 7. Postopek izdelave polsintetičnega β-laktam antibiotika, ki obsega encimsko spajanje aktivirane stranske verige z jedrom β-laktama, značilen po uporabi mutirane prokariontske penicilin G acilaze po kateremkoli zahtevku od 1 do 2.
- 8. Postopek po zahtevku 7, kjer je polsintetični β-laktam antibiotik izbran iz skupine, ki vsebuje polsintetične peniciline in polsintetične cefalosporine.
- 9. Postopek po zahtevku 8, kjer je polsintetični penicilin amoksicilin ali ampicilin.
- 10. Postopek po zahtevku 8, kjer je polsintetični cefalosporin cefaleksin, cefadroksil ali cefradin.
- 11. Postopek izdelave amoksicilina v prisotnosti acilaze, kot je opisana v kateremkoli zahtevku od 1 do 2, ki katalizira HPG v aktivirani obliki na 6-APA, kjer je molsko razmerje aktiviranega HPG na 6-APA < 3.0 in je donos amoksicilina na osnovi 6-APA (mol/mol) in merjen po reakciji encimskega spajanja >90%.
- 12. Postopek po zahtevku 11, kjer ima acilaza lastnost proizvodnje amoksicilina od 500 mmol na liter HPMG in 530 mmol na liter 6-APA in na koncu pretvorbe, ki poteka pri pogojih opisanih v testu A, nastane manj kot 5 mmol na liter 6-APA.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP08172831 | 2008-12-23 | ||
| EP09796734.3A EP2367940B1 (en) | 2008-12-23 | 2009-12-22 | Mutant penicillin G acylases |
| PCT/EP2009/067752 WO2010072765A2 (en) | 2008-12-23 | 2009-12-22 | Mutant penicillin g acylases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SI2367940T1 true SI2367940T1 (sl) | 2018-04-30 |
Family
ID=40585574
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SI200931814T SI2367940T1 (sl) | 2008-12-23 | 2009-12-22 | Mutirane penicilin G acilaze |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US8541199B2 (sl) |
| EP (1) | EP2367940B1 (sl) |
| JP (1) | JP2012513196A (sl) |
| KR (1) | KR101735126B1 (sl) |
| CN (2) | CN103642780B (sl) |
| BR (1) | BRPI0922510B8 (sl) |
| DK (1) | DK2367940T3 (sl) |
| HR (1) | HRP20180318T1 (sl) |
| HU (1) | HUE036302T2 (sl) |
| LT (1) | LT2367940T (sl) |
| MX (1) | MX2011006802A (sl) |
| NO (1) | NO2367940T3 (sl) |
| SG (1) | SG171964A1 (sl) |
| SI (1) | SI2367940T1 (sl) |
| WO (1) | WO2010072765A2 (sl) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2614066B1 (en) | 2010-09-07 | 2017-11-29 | DSM Sinochem Pharmaceuticals Netherlands B.V. | Process for the production of cephalosporins |
| US20150112057A1 (en) * | 2011-06-23 | 2015-04-23 | Dsm Sinochem Pharmaceuticals Netherlands B.V. | Novel crystalline cefoperazone intermediate |
| PL2723882T3 (pl) * | 2011-06-23 | 2020-01-31 | Centrient Pharmaceuticals Netherlands B.V. | Sposób wytwarzania 3’-tiopodstawionych cefalosporyn z zastosowaniem acylazy penicyliny |
| WO2012175587A2 (en) | 2011-06-23 | 2012-12-27 | Dsm Sinochem Pharmaceuticals Netherlands B.V. | Novel crystalline cefoperazone intermediate |
| WO2013057197A1 (en) | 2011-10-20 | 2013-04-25 | Dsm Sinochem Pharmaceuticals Netherlands B.V. | Process for the formylation of cefamandole |
| WO2013057196A1 (en) | 2011-10-20 | 2013-04-25 | Dsm Sinochem Pharmaceuticals Netherlands B.V. | Process for the preparation of cefamandole nafate |
| RU2564578C2 (ru) * | 2012-04-25 | 2015-10-10 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) | МУТАНТ ПЕНИЦИЛЛИНАЦИЛАЗЫ ИЗ E.coli С УЛУЧШЕННЫМИ СВОЙСТВАМИ |
| CN103074320B (zh) * | 2012-05-25 | 2018-06-08 | 石药集团中奇制药技术（石家庄）有限公司 | 含有一个或几个点突变的青霉素g酰化酶 |
| CN103667418B (zh) * | 2013-12-18 | 2015-03-25 | 华北制药河北华民药业有限责任公司 | 高活性β-内酰胺抗生素合成用酶的高通量筛选方法 |
| SG11201708356PA (en) * | 2015-05-07 | 2017-11-29 | Codexis Inc | Penicillin-g acylases |
| CN105087533B (zh) * | 2015-09-30 | 2018-03-27 | 湖南福来格生物技术有限公司 | 一种青霉素g酰化酶的突变体及其制备方法和应用 |
| CN105483105B (zh) * | 2016-02-23 | 2018-08-31 | 上海星维生物技术有限公司 | 一种青霉素g酰化酶突变体 |
| WO2017186864A1 (en) | 2016-04-27 | 2017-11-02 | Sandoz Ag | Enzymatic process for the production of beta-lactam antibiotics in the presence of particulate inoculum |
| US10745681B2 (en) * | 2017-01-05 | 2020-08-18 | Codexis, Inc. | Penicillin-G acylases |
| CN108660127B (zh) * | 2017-03-27 | 2021-09-21 | 珠海联邦制药股份有限公司 | 一种人工设计的青霉素g酰化酶原及其编码序列与应用 |
| CN107099523B (zh) * | 2017-06-15 | 2019-09-27 | 清华大学 | 头孢拉定合成酶突变体及其编码基因 |
| KR101985911B1 (ko) * | 2017-12-28 | 2019-06-04 | 아미코젠주식회사 | Achromobacter sp. CCM 4824 유래 페니실린 G 아실라제 변이체 및 이의 이용 |
| KR102363768B1 (ko) * | 2019-11-15 | 2022-02-16 | 아미코젠주식회사 | 세파졸린 생산성이 증가된 페니실린 g 아실라제 변이체 및 이의 이용 |
| KR20220125219A (ko) * | 2020-01-08 | 2022-09-14 | 페르멘타 바이오테크 리미티드 | 아크로모박터 ccm4824의 돌연변이 페니실린 g 아실라아제 |
| CN111500564B (zh) * | 2020-06-02 | 2022-01-18 | 南京工业大学 | 青霉素g酰化酶突变体及其在酶法合成头孢孟多中的应用 |
| WO2022195603A1 (en) * | 2021-03-18 | 2022-09-22 | Fermenta Biotech Limited | A ONE STEP PROCESS FOR THE ENZYMATIC SYNTHESIS OF SEMISYNTHETIC β-LACTAM ANTIBIOTICS |
| CN113567607A (zh) * | 2021-07-23 | 2021-10-29 | 河北冀安检测服务有限公司 | 一种头孢克洛中青霉素g酰基转移酶含量的检测方法 |
| CN113584008B (zh) * | 2021-07-30 | 2023-04-11 | 湖南福来格生物技术有限公司 | 一种青霉素g酰化酶突变体及其应用 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT83746B (pt) | 1985-11-15 | 1988-08-17 | Gist Brocades Nv | Processo para a preparacao de novos biocatalisadores imobilizados e para a producao de etanol por fermentacao |
| ATE189477T1 (de) * | 1994-08-12 | 2000-02-15 | Dsm Nv | Mutierte penicillin-g-acylase gene |
| CN1572873A (zh) * | 1995-07-18 | 2005-02-02 | 吉斯特·布罗卡迪斯股份有限公司 | 一种改进的固定化青霉素g酰基转移酶 |
| IL129055A (en) * | 1996-11-05 | 2005-06-19 | Bristol Myers Squibb Co | Mutant penicillin g acylases |
| US6562958B1 (en) * | 1998-06-09 | 2003-05-13 | Genome Therapeutics Corporation | Nucleic acid and amino acid sequences relating to Acinetobacter baumannii for diagnostics and therapeutics |
| CZ291154B6 (cs) | 2001-08-03 | 2002-12-11 | Mikrobiologický Ústav Av Čr | Kmen mikroorganismu Comamonas testosteroni CCM 4824 |
| BR0215329A (pt) * | 2001-12-27 | 2004-11-16 | Dsm Ip Assets Bv | Processo para preparação de um antibiótico de b-lactama |
| DE10251747A1 (de) | 2002-11-05 | 2004-05-19 | Tuhh-Technologie-Gmbh | Gentechnisch veränderte Penicillinamidase und Verfahren zu ihrer Herstellung |
| LT1641933T (lt) * | 2003-07-03 | 2018-04-10 | Dsm Sinochem Pharmaceuticals Netherlands B.V. | Cefradino gamybos būdas |
| CN100507000C (zh) * | 2003-07-03 | 2009-07-01 | 帝斯曼知识产权资产管理有限公司 | 制备头孢拉定的方法 |
| JP5022913B2 (ja) * | 2004-12-27 | 2012-09-12 | ディーエスエム シノケム ファーマシューティカルズ ネザーランズ ビー．ヴイ． | セファクロルの合成方法 |
| CN101177688B (zh) | 2006-11-08 | 2010-12-01 | 中国科学院上海生命科学研究院 | 突变青霉素g酰化酶、其重组表达质粒及转化的工程菌株 |
| US8497088B2 (en) | 2007-03-09 | 2013-07-30 | Dsm Sinochem Pharmaceuticals Netherlands B.V. | Process for the preparation of beta-lactam compounds |
-
2009
- 2009-12-22 HU HUE09796734A patent/HUE036302T2/hu unknown
- 2009-12-22 US US13/141,877 patent/US8541199B2/en active Active
- 2009-12-22 CN CN201310325865.0A patent/CN103642780B/zh active Active
- 2009-12-22 CN CN2009801524656A patent/CN102264904B/zh active Active
- 2009-12-22 JP JP2011541511A patent/JP2012513196A/ja active Pending
- 2009-12-22 SI SI200931814T patent/SI2367940T1/sl unknown
- 2009-12-22 DK DK09796734.3T patent/DK2367940T3/en active
- 2009-12-22 NO NO09796734A patent/NO2367940T3/no unknown
- 2009-12-22 LT LTEP09796734.3T patent/LT2367940T/lt unknown
- 2009-12-22 KR KR1020117017104A patent/KR101735126B1/ko active IP Right Grant
- 2009-12-22 MX MX2011006802A patent/MX2011006802A/es active IP Right Grant
- 2009-12-22 WO PCT/EP2009/067752 patent/WO2010072765A2/en active Application Filing
- 2009-12-22 SG SG2011040888A patent/SG171964A1/en unknown
- 2009-12-22 EP EP09796734.3A patent/EP2367940B1/en active Active
- 2009-12-22 BR BRPI0922510A patent/BRPI0922510B8/pt active IP Right Grant
-
2018
- 2018-02-21 HR HRP20180318TT patent/HRP20180318T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2010072765A2 (en) | 2010-07-01 |
| LT2367940T (lt) | 2018-03-26 |
| KR20110099137A (ko) | 2011-09-06 |
| JP2012513196A (ja) | 2012-06-14 |
| EP2367940A2 (en) | 2011-09-28 |
| US8541199B2 (en) | 2013-09-24 |
| SG171964A1 (en) | 2011-07-28 |
| CN103642780A (zh) | 2014-03-19 |
| BRPI0922510B8 (pt) | 2021-05-25 |
| BRPI0922510B1 (pt) | 2021-01-19 |
| DK2367940T3 (en) | 2018-03-05 |
| CN102264904B (zh) | 2013-10-23 |
| BRPI0922510A2 (pt) | 2015-08-04 |
| US20110256585A1 (en) | 2011-10-20 |
| HUE036302T2 (hu) | 2018-07-30 |
| HRP20180318T1 (hr) | 2018-03-23 |
| EP2367940B1 (en) | 2017-11-22 |
| KR101735126B1 (ko) | 2017-05-12 |
| NO2367940T3 (sl) | 2018-04-21 |
| CN103642780B (zh) | 2017-06-20 |
| WO2010072765A3 (en) | 2010-08-26 |
| MX2011006802A (es) | 2011-07-20 |
| CN102264904A (zh) | 2011-11-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SI2367940T1 (sl) | Mutirane penicilin G acilaze | |
| US5891703A (en) | Mutated penicillin G acylase genes | |
| KR100193802B1 (ko) | 돌연변이된 베타-락탐 아실라아제 유전자 | |
| KR20040075042A (ko) | β-락탐 항생제의 제조 방법 | |
| EP0961825B1 (en) | Mutant penicillin g acylases | |
| WO2017143944A1 (zh) | 一种青霉素g酰化酶突变体 | |
| Becka et al. | Penicillin G acylase from Achromobacter sp. CCM 4824 | |
| CN106399174B (zh) | 一株青霉素酰化酶及其编码基因、产生菌和应用 | |
| EP1186668A1 (en) | An enzymatic process for preparing Beta-lactam compounds | |
| US20150240282A1 (en) | Mutated cephalosporin hydroxylase and its application in deacetylcephalosporanic acid synthesis | |
| WO2004111241A1 (en) | Penicillin acylase | |
| HUP0301202A2 (hu) | Glutaril-cefalosporin amidáz Pseudomonas diminuta BS-203-ból | |
| EP0638649A2 (en) | New use of alcaligenes faecalis penicillin G acylase | |
| US20030044884A1 (en) | Synthesis of beta-lactam antibacterials using soluble side chain esters and enzyme acylase | |
| Samanta | Enzymatic synthesis of ß-lactams: Constraints and control | |
| RU2537845C2 (ru) | Способ синтеза пептидов, в том числе бета-лактамных антибиотиков, при использовании варианта пенициллинацилазы | |
| CN115851786A (zh) | 一种编码青霉素g酰化酶的基因及表达的突变后青霉素g酰化酶在制备头孢氨苄中应用 | |
| EP0918879A1 (en) | A METHOD FOR PREPARING A $g(b)-LACTAM ANTIBIOTIC | |
| GB1480850A (en) | Enzymatic hydrolysis and synthesis | |
| EP1538205A1 (en) | Glutaryl amidases and their uses | |
| Grulich et al. | Helena Marešová, Martina Plačková | |
| KANG et al. | One-step Purification of Poly-His Tagged Penicillin G Acylase Expressed inE. coli | |
| MXPA98000406A (en) | An acilase of penicillin g immobilized, better |