SG185453A1 - Biologic female contraceptives - Google Patents
Biologic female contraceptives Download PDFInfo
- Publication number
- SG185453A1 SG185453A1 SG2012081931A SG2012081931A SG185453A1 SG 185453 A1 SG185453 A1 SG 185453A1 SG 2012081931 A SG2012081931 A SG 2012081931A SG 2012081931 A SG2012081931 A SG 2012081931A SG 185453 A1 SG185453 A1 SG 185453A1
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- SG
- Singapore
- Prior art keywords
- sperm
- engineered
- bacterium
- engineered bacterium
- female
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/18—Feminine contraceptives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2210/00—Anatomical parts of the body
- A61M2210/14—Female reproductive, genital organs
- A61M2210/1475—Vagina
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US33189210P | 2010-05-06 | 2010-05-06 | |
IL208820A IL208820A0 (en) | 2010-10-19 | 2010-10-19 | Biologic female contraceptives |
PCT/IL2011/000352 WO2011138776A2 (en) | 2010-05-06 | 2011-05-04 | Biologic female contraceptives |
Publications (1)
Publication Number | Publication Date |
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SG185453A1 true SG185453A1 (en) | 2012-12-28 |
Family
ID=44718589
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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SG2012081931A SG185453A1 (en) | 2010-05-06 | 2011-05-04 | Biologic female contraceptives |
Country Status (21)
Country | Link |
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US (1) | US8668903B2 (ja) |
EP (1) | EP2566490B1 (ja) |
JP (3) | JP2013527763A (ja) |
KR (1) | KR101927747B1 (ja) |
CN (2) | CN102971007A (ja) |
AP (1) | AP2012006598A0 (ja) |
AU (2) | AU2011249472B2 (ja) |
BR (1) | BR112012028427A2 (ja) |
CA (1) | CA2797082C (ja) |
CL (1) | CL2012003105A1 (ja) |
CO (1) | CO6640222A2 (ja) |
EA (1) | EA201291177A1 (ja) |
ES (1) | ES2671345T3 (ja) |
IL (2) | IL208820A0 (ja) |
MX (1) | MX2012012576A (ja) |
NZ (1) | NZ603450A (ja) |
PE (1) | PE20130577A1 (ja) |
SG (1) | SG185453A1 (ja) |
TR (1) | TR201807468T4 (ja) |
WO (1) | WO2011138776A2 (ja) |
ZA (1) | ZA201208328B (ja) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9616114B1 (en) | 2014-09-18 | 2017-04-11 | David Gordon Bermudes | Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
US20230002785A1 (en) * | 2019-10-28 | 2023-01-05 | Georgia Tech Research Corporation | Mrna-encoded antibodies for contraception |
CN110934895A (zh) * | 2019-12-26 | 2020-03-31 | 南昌大学 | 卷曲乳酸杆菌用于制备女性避孕药物的应用 |
Family Cites Families (66)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NL154600B (nl) | 1971-02-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen. |
NL154598B (nl) | 1970-11-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking. |
NL154599B (nl) | 1970-12-28 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking. |
US3901654A (en) | 1971-06-21 | 1975-08-26 | Biological Developments | Receptor assays of biologically active compounds employing biologically specific receptors |
US3853987A (en) | 1971-09-01 | 1974-12-10 | W Dreyer | Immunological reagent and radioimmuno assay |
US3867517A (en) | 1971-12-21 | 1975-02-18 | Abbott Lab | Direct radioimmunoassay for antigens and their antibodies |
NL171930C (nl) | 1972-05-11 | 1983-06-01 | Akzo Nv | Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen. |
US3850578A (en) | 1973-03-12 | 1974-11-26 | H Mcconnell | Process for assaying for biologically active molecules |
US4179337A (en) | 1973-07-20 | 1979-12-18 | Davis Frank F | Non-immunogenic polypeptides |
US3935074A (en) | 1973-12-17 | 1976-01-27 | Syva Company | Antibody steric hindrance immunoassay with two antibodies |
US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
US4034074A (en) | 1974-09-19 | 1977-07-05 | The Board Of Trustees Of Leland Stanford Junior University | Universal reagent 2-site immunoradiometric assay using labelled anti (IgG) |
US3984533A (en) | 1975-11-13 | 1976-10-05 | General Electric Company | Electrophoretic method of detecting antigen-antibody reaction |
US4098876A (en) | 1976-10-26 | 1978-07-04 | Corning Glass Works | Reverse sandwich immunoassay |
US4322399A (en) | 1979-10-19 | 1982-03-30 | Ortho Pharmaceutical Corporation | Vaginal suppository |
US4444887A (en) | 1979-12-10 | 1984-04-24 | Sloan-Kettering Institute | Process for making human antibody producing B-lymphocytes |
US4879219A (en) | 1980-09-19 | 1989-11-07 | General Hospital Corporation | Immunoassay utilizing monoclonal high affinity IgM antibodies |
US4693705A (en) | 1984-04-09 | 1987-09-15 | Gero Ilona B | Vaginal contraceptive system |
US5011771A (en) | 1984-04-12 | 1991-04-30 | The General Hospital Corporation | Multiepitopic immunometric assay |
US4666828A (en) | 1984-08-15 | 1987-05-19 | The General Hospital Corporation | Test for Huntington's disease |
JPS61210285A (ja) | 1985-03-14 | 1986-09-18 | Toshiba Corp | 回転式圧縮機 |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4801531A (en) | 1985-04-17 | 1989-01-31 | Biotechnology Research Partners, Ltd. | Apo AI/CIII genomic polymorphisms predictive of atherosclerosis |
US5227160A (en) | 1988-07-15 | 1993-07-13 | The Biomembrane Institute | Monoclonal antibody nuh2 capable of inactivating motility of human sperm, antigen defined by said monoclonal antibody and methods of using said monoclonal antibody and antigen |
US4922928A (en) | 1988-08-17 | 1990-05-08 | Michael Burnhill | Vaginal device |
US5272057A (en) | 1988-10-14 | 1993-12-21 | Georgetown University | Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase |
US5602005A (en) | 1989-03-03 | 1997-02-11 | The University Of Virginia Alumni Patents Foundation | Primate intra-acrosomal sperm antigen for use in a contraceptive vaccine |
US5413923A (en) | 1989-07-25 | 1995-05-09 | Cell Genesys, Inc. | Homologous recombination for universal donor cells and chimeric mammalian hosts |
US5192659A (en) | 1989-08-25 | 1993-03-09 | Genetype Ag | Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes |
DE69120146T2 (de) | 1990-01-12 | 1996-12-12 | Cell Genesys Inc | Erzeugung xenogener antikörper |
GB9015198D0 (en) | 1990-07-10 | 1990-08-29 | Brien Caroline J O | Binding substance |
US5633425A (en) | 1990-08-29 | 1997-05-27 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
US5545806A (en) | 1990-08-29 | 1996-08-13 | Genpharm International, Inc. | Ransgenic non-human animals for producing heterologous antibodies |
US5814318A (en) | 1990-08-29 | 1998-09-29 | Genpharm International Inc. | Transgenic non-human animals for producing heterologous antibodies |
US5661016A (en) | 1990-08-29 | 1997-08-26 | Genpharm International Inc. | Transgenic non-human animals capable of producing heterologous antibodies of various isotypes |
US5625126A (en) | 1990-08-29 | 1997-04-29 | Genpharm International, Inc. | Transgenic non-human animals for producing heterologous antibodies |
DK0814159T3 (da) | 1990-08-29 | 2005-10-24 | Genpharm Int | Transgene, ikke-humane dyr, der er i stand til at danne heterologe antistoffer |
ES2313867T3 (es) | 1991-12-02 | 2009-03-16 | Medical Research Council | Produccion de anticuerpos anti-auto de repertorios de segmentos de anticuerpo expresados en la superficie de fagos. |
JP3626181B2 (ja) * | 1992-03-13 | 2005-03-02 | ザ ロックフェラー ユニバーシティー | グラム陽性菌の表面上のハイブリッド表面タンパク質の供給及び発現 |
US5393528A (en) | 1992-05-07 | 1995-02-28 | Staab; Robert J. | Dissolvable device for contraception or delivery of medication |
HU218069B (hu) | 1992-06-09 | 2000-05-28 | Hoppe Ag. | Kilincses zárrendszer |
US5281521A (en) | 1992-07-20 | 1994-01-25 | The Trustees Of The University Of Pennsylvania | Modified avidin-biotin technique |
ZA947572B (en) | 1993-09-29 | 1995-09-28 | Hampton Roads Medical College | Contraceptive compositions |
US5733540A (en) | 1995-03-08 | 1998-03-31 | Lee; Peter Poon-Hang | Protection from viral infection via colonization of mucosal membranes with genetically modified bacteria |
EP1709970A1 (en) | 1995-04-27 | 2006-10-11 | Abgenix, Inc. | Human antibodies against EGFR, derived from immunized xenomice |
AU2466895A (en) | 1995-04-28 | 1996-11-18 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
US5830472A (en) | 1996-06-28 | 1998-11-03 | The University Of Virginia Patent Foundation | Purified sperm surface antigen, monoclonal antibody therefor and applications therefor |
US5916771A (en) | 1996-10-11 | 1999-06-29 | Abgenix, Inc. | Production of a multimeric protein by cell fusion method |
CA2273194C (en) | 1996-12-03 | 2011-02-01 | Abgenix, Inc. | Transgenic mammals having human ig loci including plural vh and vk regions and antibodies produced therefrom |
US6093394A (en) | 1997-04-11 | 2000-07-25 | Gynelogix, Inc. | Vaginal lactobacillus medicant |
TR199902553T2 (xx) | 1997-04-14 | 2000-03-21 | Micromet Gesellschaft F�R Biomedizinische Forschung Mbh | �nsan v�cuduna kar�� antijen resept�rlerinin �retimi i�in yeni metod ve kullan�mlar�. |
US6207384B1 (en) | 1998-03-27 | 2001-03-27 | The General Hospital Corporation | Systematic identification of essential genes by in vitro transposon mutagenesis |
ATE423215T1 (de) | 1998-03-31 | 2009-03-15 | Takara Bio Inc | Verfahren zur herstellung von lysosphingolipiden |
JP2002526425A (ja) * | 1998-10-05 | 2002-08-20 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 粘膜免疫を刺激するための方法およびアジュバント |
WO2000078922A2 (en) | 1999-06-21 | 2000-12-28 | North Carolina State University | Acid-inducible promoters for gene expression |
WO2001007083A1 (en) * | 1999-07-23 | 2001-02-01 | University Of Virginia Patent Foundation | Recombinant antibody directed against human sperm antigen |
WO2004007695A2 (en) * | 2002-03-08 | 2004-01-22 | Osel, Inc. | Lactobacilli expressing biologically active polypeptides and uses thereof |
EP1590443A4 (en) * | 2003-01-29 | 2009-01-07 | Osel Inc | SURFACE EXPRESSION OF BIOLOGICALLY ACTIVE PROTEINS IN BACTERIA |
US20050191318A1 (en) * | 2004-02-27 | 2005-09-01 | Kitto G. B. | DNA immunocontraceptive vaccines and uses thereof |
WO2005112567A2 (en) | 2004-05-03 | 2005-12-01 | University Of Maryland Biotechnology Institute | Vaginal microbicide |
EP1786445B1 (en) * | 2004-08-25 | 2011-06-08 | The Government of the United States of America as represented by The Secretary of the Department of Health and Human Services | Live microbial microbicides |
JP2008533986A (ja) * | 2005-03-25 | 2008-08-28 | ラモト アット テル アヴィヴ ユニヴァーシティ リミテッド | 変異型p53の共通エピトープに対するヒト合成単鎖抗体およびその使用 |
EA014527B1 (ru) * | 2005-03-31 | 2010-12-30 | Глаксосмитклайн Байолоджикалс С.А. | Вакцины против хламидиоза |
WO2009062034A1 (en) * | 2007-11-07 | 2009-05-14 | Auburn University | Methods, compositions, and sequences of zp-binding peptides for immunocontraception of dogs and other animals |
CN103293689B (zh) | 2013-05-31 | 2015-05-13 | 京东方科技集团股份有限公司 | 一种可在不同显示模式之间切换的方法和显示装置 |
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- 2011-05-04 PE PE2012002123A patent/PE20130577A1/es not_active Application Discontinuation
- 2011-05-04 CA CA2797082A patent/CA2797082C/en active Active
- 2011-05-04 WO PCT/IL2011/000352 patent/WO2011138776A2/en active Application Filing
- 2011-05-04 BR BR112012028427A patent/BR112012028427A2/pt not_active IP Right Cessation
- 2011-05-04 AU AU2011249472A patent/AU2011249472B2/en not_active Ceased
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- 2011-05-04 ES ES11777356.4T patent/ES2671345T3/es active Active
- 2011-05-04 US US13/256,690 patent/US8668903B2/en active Active
- 2011-05-04 EP EP11777356.4A patent/EP2566490B1/en active Active
- 2011-05-04 JP JP2013508611A patent/JP2013527763A/ja active Pending
- 2011-05-04 TR TR2018/07468T patent/TR201807468T4/en unknown
- 2011-05-04 SG SG2012081931A patent/SG185453A1/en unknown
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