SG169359A1 - A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses - Google Patents
A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetusesInfo
- Publication number
- SG169359A1 SG169359A1 SG201100658-2A SG2011006582A SG169359A1 SG 169359 A1 SG169359 A1 SG 169359A1 SG 2011006582 A SG2011006582 A SG 2011006582A SG 169359 A1 SG169359 A1 SG 169359A1
- Authority
- SG
- Singapore
- Prior art keywords
- condition
- cardiomyocytes
- stem cells
- supplemented
- fetuses
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0657—Cardiomyocytes; Heart cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/02—Atmosphere, e.g. low oxygen conditions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
- C12N2500/14—Calcium; Ca chelators; Calcitonin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/34—Sugars
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/44—Thiols, e.g. mercaptoethanol
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/99—Serum-free medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/235—Leukemia inhibitory factor [LIF]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2006023770 | 2006-01-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG169359A1 true SG169359A1 (en) | 2011-03-30 |
Family
ID=38327445
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG201100658-2A SG169359A1 (en) | 2006-01-31 | 2007-01-31 | A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US9115342B2 (zh) |
| EP (2) | EP2468851A1 (zh) |
| JP (2) | JP5312804B2 (zh) |
| KR (2) | KR101348325B1 (zh) |
| CN (2) | CN103409368B (zh) |
| AU (1) | AU2007210580B2 (zh) |
| BR (1) | BRPI0706801B8 (zh) |
| CA (1) | CA2640644C (zh) |
| IL (1) | IL193095A (zh) |
| RU (1) | RU2426784C2 (zh) |
| SG (1) | SG169359A1 (zh) |
| WO (1) | WO2007088874A1 (zh) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3354723B1 (en) | 2005-08-29 | 2023-12-13 | Technion Research & Development Foundation Ltd. | Media for culturing stem cells |
| CA2640644C (en) * | 2006-01-31 | 2013-11-26 | Asubio Pharma Co., Ltd. | A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses |
| DK3441459T3 (da) | 2006-08-02 | 2021-06-07 | Technion Res & Dev Foundation | Fremgangsmåder til ekspansion af embryonale stamceller i en suspensionskultur |
| CN102449139B (zh) | 2009-03-30 | 2016-08-24 | 第一三共株式会社 | 对多能性干细胞和心肌细胞以外的分化细胞诱导细胞死亡的方法 |
| EP4166652A1 (en) | 2009-11-12 | 2023-04-19 | Technion Research & Development Foundation Ltd. | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
| CA2802525C (en) * | 2010-06-18 | 2021-06-22 | Cellular Dynamics International, Inc. | Cardiomyocyte medium with dialyzed serum |
| KR101364965B1 (ko) * | 2010-10-06 | 2014-02-18 | (주)차바이오앤디오스텍 | 인간 배아줄기세포 유래 심근세포의 배양 및 정제 방법 |
| JP5840855B2 (ja) | 2011-03-30 | 2016-01-06 | 学校法人東京女子医科大学 | 胚性幹細胞から心筋シートを製造する方法 |
| DK2737056T3 (en) * | 2011-07-29 | 2018-05-22 | Cellular Dynamics Int Inc | Metabolic maturation in stem cell-derived tissue cells |
| ES2523903B1 (es) * | 2013-04-30 | 2015-09-09 | Fundación Pública Andaluza Progreso Y Salud | Método de obtención de datos útiles para el diagnóstico diferencial de la fibrosis hepática |
| CN105039399A (zh) * | 2014-04-23 | 2015-11-11 | 复旦大学 | 多能干细胞-遗传性心肌病心肌细胞及其制备方法 |
| KR102241348B1 (ko) | 2014-07-16 | 2021-04-15 | 하트 시드 가부시키가이샤 | 신규 미분화 줄기세포 제거 및 심근 순화 정제 배지 |
| EP3196297B1 (en) | 2014-09-16 | 2020-11-04 | Osaka University | Method for producing cardiomyocyte population from pluripotent stem cells |
| SG10201903910RA (en) * | 2014-11-07 | 2019-05-30 | Univ Osaka | Differentiation-induced cell population from which undifferentiated cells have been removed, use of same, and method for producing same |
| JP6622007B2 (ja) * | 2015-05-29 | 2019-12-18 | オリンパス株式会社 | 細胞評価方法 |
| US11390851B2 (en) | 2016-06-01 | 2022-07-19 | Miltenyi Biotec B.V. & Co. KG | Process for generation, identification and isolation of human pluripotent stem cell-derived cardiomyocytes and cardiomyocyte subpopulations |
| CN106244527B (zh) * | 2016-08-29 | 2019-10-08 | 广东依浦赛生物科技有限公司 | 人源iPS干细胞体外定向分化为心肌细胞的试剂盒及方法 |
| AU2017346898B2 (en) * | 2016-10-17 | 2021-04-01 | Keio University | Undifferentiated stem cell-removing agent, and method for removing undifferentiated stem cells |
| CN109153963B (zh) | 2016-11-01 | 2022-06-24 | 泰尔茂株式会社 | 粘附状态的细胞培养物的改变方法 |
| CN106754663A (zh) * | 2016-11-30 | 2017-05-31 | 中国人民解放军第三军医大学第三附属医院 | 促进成年哺乳动物心肌细胞存活的体外培养方法及监测成年哺乳动物心肌细胞增殖的方法 |
| JP2018093823A (ja) | 2016-12-15 | 2018-06-21 | Ｈｅａｒｔｓｅｅｄ株式会社 | 未分化幹細胞除去剤及び未分化幹細胞除去方法 |
| CN110506109B (zh) | 2017-06-05 | 2024-03-12 | 泰尔茂株式会社 | 细胞培养物的制造方法 |
| CN107828721A (zh) * | 2017-11-10 | 2018-03-23 | 山东农业大学 | 一种鸡胚心肌细胞的培养及免疫组织化学鉴定方法 |
| JPWO2020067436A1 (ja) * | 2018-09-27 | 2021-08-30 | 国立大学法人大阪大学 | 多能性幹細胞由来細胞の移植片形成方法 |
| WO2020067438A1 (ja) | 2018-09-27 | 2020-04-02 | 国立大学法人大阪大学 | 多能性幹細胞由来細胞のシート化方法 |
| JPWO2020067435A1 (ja) * | 2018-09-27 | 2021-09-09 | 国立大学法人大阪大学 | 多能性幹細胞由来細胞のシート化方法 |
| CN110904024A (zh) * | 2019-11-13 | 2020-03-24 | 武汉济源高科技有限公司 | 一种悬浮细胞培养中去除死细胞的方法 |
| CN111621470B (zh) * | 2020-06-08 | 2023-06-27 | 广东源心再生医学有限公司 | 一种高效低毒的心肌纯化培养基及方法 |
| WO2024008773A1 (en) | 2022-07-05 | 2024-01-11 | Repairon Gmbh | Differentiation of ipscs in bioreactors |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL47012C (zh) | 1933-03-23 | |||
| GB9903804D0 (en) | 1999-02-20 | 1999-04-14 | Univ Sheffield | Pluripotential cells-2 |
| AU784618B2 (en) | 1999-12-28 | 2006-05-18 | Kyowa Hakko Kogyo Co. Ltd. | Cells capable of differentiating into heart muscle cells |
| CA2430627A1 (en) * | 2000-11-30 | 2002-12-27 | Stemron, Inc. | Isolated homozygous stem cells differentiated cells derived therefrom and materials and methods for making and using same |
| US7732199B2 (en) * | 2001-07-12 | 2010-06-08 | Geron Corporation | Process for making transplantable cardiomyocytes from human embryonic stem cells |
| CA2453438C (en) * | 2001-07-12 | 2016-04-05 | Geron Corporation | Cells of the cardiomyocyte lineage produced from human pluripotent stem cells |
| EP1725654B1 (en) * | 2004-03-19 | 2019-05-01 | Asterias Biotherapeutics, Inc. | Method for making high purity cardiomyocyte preparations suitable for regenerative medicine |
| CA2569242A1 (en) * | 2004-06-01 | 2005-12-15 | Es Cell International Pte Ltd | Improved cardiomyocyte differentiation |
| US20080254003A1 (en) | 2004-12-22 | 2008-10-16 | Robert Passier | Differentiation of Human Embryonic Stem Cells and Cardiomyocytes and Cardiomyocyte Progenitors Derived Therefrom |
| CA2640644C (en) * | 2006-01-31 | 2013-11-26 | Asubio Pharma Co., Ltd. | A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses |
-
2007
- 2007-01-31 CA CA2640644A patent/CA2640644C/en active Active
- 2007-01-31 EP EP20120160317 patent/EP2468851A1/en not_active Withdrawn
- 2007-01-31 CN CN201310348017.1A patent/CN103409368B/zh active Active
- 2007-01-31 EP EP07713734.7A patent/EP1983042B1/en active Active
- 2007-01-31 BR BRPI0706801A patent/BRPI0706801B8/pt active IP Right Grant
- 2007-01-31 SG SG201100658-2A patent/SG169359A1/en unknown
- 2007-01-31 WO PCT/JP2007/051563 patent/WO2007088874A1/ja active Application Filing
- 2007-01-31 US US12/162,684 patent/US9115342B2/en active Active
- 2007-01-31 AU AU2007210580A patent/AU2007210580B2/en active Active
- 2007-01-31 RU RU2008135313A patent/RU2426784C2/ru active
- 2007-01-31 CN CNA200780003957XA patent/CN101374942A/zh active Pending
- 2007-01-31 KR KR1020087020152A patent/KR101348325B1/ko active IP Right Grant
- 2007-01-31 JP JP2007556880A patent/JP5312804B2/ja active Active
- 2007-01-31 KR KR20127027002A patent/KR101455144B1/ko active IP Right Grant
-
2008
- 2008-07-28 IL IL193095A patent/IL193095A/en active IP Right Grant
-
2013
- 2013-04-30 JP JP2013095524A patent/JP5756145B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2013143968A (ja) | 2013-07-25 |
| KR101455144B1 (ko) | 2014-10-27 |
| EP1983042B1 (en) | 2017-03-15 |
| WO2007088874A1 (ja) | 2007-08-09 |
| CN103409368B (zh) | 2016-05-25 |
| AU2007210580A1 (en) | 2007-08-09 |
| IL193095A (en) | 2015-06-30 |
| KR20120123608A (ko) | 2012-11-08 |
| RU2426784C2 (ru) | 2011-08-20 |
| KR20080097197A (ko) | 2008-11-04 |
| BRPI0706801A2 (pt) | 2011-04-05 |
| BRPI0706801B8 (pt) | 2021-05-25 |
| BRPI0706801B1 (pt) | 2020-04-07 |
| EP1983042A1 (en) | 2008-10-22 |
| CA2640644C (en) | 2013-11-26 |
| AU2007210580B2 (en) | 2012-10-11 |
| US9115342B2 (en) | 2015-08-25 |
| KR101348325B1 (ko) | 2014-01-08 |
| JPWO2007088874A1 (ja) | 2009-06-25 |
| CN103409368A (zh) | 2013-11-27 |
| EP1983042A4 (en) | 2009-06-17 |
| RU2008135313A (ru) | 2010-03-20 |
| US20090275132A1 (en) | 2009-11-05 |
| CN101374942A (zh) | 2009-02-25 |
| IL193095A0 (en) | 2009-02-11 |
| EP2468851A1 (en) | 2012-06-27 |
| CA2640644A1 (en) | 2007-08-09 |
| JP5312804B2 (ja) | 2013-10-09 |
| JP5756145B2 (ja) | 2015-07-29 |
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