SG11201810871WA - Methods of producing modified natural killer cells and methods of use - Google Patents

Methods of producing modified natural killer cells and methods of use

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Publication number
SG11201810871WA
SG11201810871WA SG11201810871WA SG11201810871WA SG11201810871WA SG 11201810871W A SG11201810871W A SG 11201810871WA SG 11201810871W A SG11201810871W A SG 11201810871WA SG 11201810871W A SG11201810871W A SG 11201810871WA SG 11201810871W A SG11201810871W A SG 11201810871WA
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Singapore
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cells
methods
international
bethesda
population
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SG11201810871WA
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English (en)
Inventor
Richard Childs
Dhanalakshmi Chinnasamy
David Allan
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Us Health
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Publication of SG11201810871WA publication Critical patent/SG11201810871WA/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0646—Natural killers cells [NK], NKT cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/15—Natural-killer [NK] cells; Natural-killer T [NKT] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N5/0068—General culture methods using substrates
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
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    • C12N2502/99—Coculture with; Conditioned medium produced by genetically modified cells
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
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    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
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    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

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SG11201810871WA 2016-07-25 2017-07-25 Methods of producing modified natural killer cells and methods of use SG11201810871WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662366493P 2016-07-25 2016-07-25
PCT/US2017/043774 WO2018022646A1 (en) 2016-07-25 2017-07-25 Methods of producing modified natural killer cells and methods of use

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Publication Number Publication Date
SG11201810871WA true SG11201810871WA (en) 2019-01-30

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SG11201810871WA SG11201810871WA (en) 2016-07-25 2017-07-25 Methods of producing modified natural killer cells and methods of use
SG11202003201QA SG11202003201QA (en) 2016-07-25 2018-11-01 Methods of producing modified natural killer cells and methods of use

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Country Status (7)

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US (2) US11293010B2 (https=)
EP (2) EP3487991B1 (https=)
JP (2) JP7086928B2 (https=)
CN (2) CN109844099B (https=)
IL (1) IL273979B2 (https=)
SG (2) SG11201810871WA (https=)
WO (2) WO2018022646A1 (https=)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015259877B2 (en) 2014-05-15 2021-02-25 National University Of Singapore Modified natural killer cells and uses thereof
US10813952B2 (en) * 2014-11-14 2020-10-27 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services NK cells with an increased antibody-dependent cellular toxicity (ADCC) against tumors
CN109844099B (zh) * 2016-07-25 2024-01-02 美国政府(由卫生和人类服务部的部长所代表) 产生经修饰的自然杀伤细胞的方法及使用方法
US12516292B2 (en) 2016-07-25 2026-01-06 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods of producing modified natural killer cells and methods of use
WO2018104554A1 (en) * 2016-12-09 2018-06-14 Onkimmune Limited Improved nk-based cell therapy
CN110546265A (zh) 2017-02-09 2019-12-06 因达普塔治疗公司 工程化自然杀伤(nk)细胞及其组合物和方法
MX2019011570A (es) 2017-03-27 2019-11-18 Nat Univ Singapore Lineas celulares estimuladoras para expansion y activacion ex vivo de celulas asesinas naturales.
KR102660336B1 (ko) 2017-03-27 2024-04-26 내셔널 유니버시티 오브 싱가포르 절단된 nkg2d 키메라 수용체 및 자연 살해 세포 면역요법에서의 그의 용도
EP3746095A4 (en) 2018-02-01 2021-04-21 Nkmax Co., Ltd. Method of producing natural killer cells and composition for treating cancer
AU2019219454A1 (en) * 2018-02-09 2020-08-27 National University Of Singapore Activating chimeric receptors and uses thereof in natural killer cell immunotherapy
PT3755347T (pt) * 2018-02-21 2025-07-25 Univ Texas Células de apresentação de antigénio universais e utilizações destas
JP7273421B2 (ja) 2018-02-21 2023-05-15 ボード オブ リージェンツ，ザ ユニバーシティ オブ テキサス システム ナチュラルキラー細胞の活性化および拡大のための方法ならびにその使用
GB201804701D0 (en) * 2018-03-23 2018-05-09 Gammadelta Therapeutics Ltd Lymphocytes expressing heterologous targeting constructs
CN112055717B (zh) 2018-04-02 2024-04-26 新加坡国立大学 用在免疫细胞中表达的膜结合抗细胞因子非信号传导粘合剂中和人细胞因子
IL278723B2 (en) * 2018-05-16 2024-07-01 Res Inst Nationwide Childrens Hospital Generation of knock-out primary and expanded human nk cells using cas9 ribonucleoproteins
AU2019275067A1 (en) * 2018-05-23 2020-12-17 Manysmart Therapeutics, Inc. Bispecific T cell engager and uses thereof
JP2021525066A (ja) * 2018-05-30 2021-09-24 グリコステム セラピューティクス ベスローテン フェンノートシャップ Ｃａｒ ｎｋ細胞
US20210230542A1 (en) * 2018-06-06 2021-07-29 Stemcell Technologies Canada Inc. Kits, compositions and methods for cell enrichment
WO2020028656A1 (en) 2018-08-01 2020-02-06 Nantkwest, Inc. A quadricistronic system comprising a homing receptor or a cytokine, and chimeric antigen receptor for genetic modification of immunotherapies
CA3110922A1 (en) * 2018-08-28 2020-03-05 Immunotech Biopharm Co., Ltd. Improved therapeutic t cell
WO2020042648A1 (zh) * 2018-08-28 2020-03-05 法罗斯疫苗株式会社 改进的慢病毒载体
EP3844186A4 (en) 2018-08-29 2022-08-17 National University of Singapore METHOD OF SPECIFIC STIMULATION OF SURVIVAL AND EXPANSION OF GENETICALLY MODIFIED IMMUNE CELLS
CN110511912B (zh) * 2018-08-30 2024-03-22 浙江煦顼技术有限公司 免疫细胞的功能调节
WO2020077091A1 (en) * 2018-10-10 2020-04-16 Wisconsin Alumni Research Foundation Kir 7.1 gene therapy vectors and methods of using the same
CN109402053A (zh) * 2018-10-17 2019-03-01 广州元帅生物科技有限公司 一种外周血来源单个核细胞的分离及诱导培养方法
US12410402B2 (en) * 2018-11-21 2025-09-09 Indapta Therapeutics, Inc. Methods for expansion of natural killer (NK) cell subset and related compositions and methods
US12590148B2 (en) 2018-11-26 2026-03-31 Nkarta, Inc. Methods for the simultaneous expansion of multiple immune cell types, related compositions and uses of same in cancer immunotherapy
EP3927730A4 (en) * 2019-02-20 2022-12-21 Rutgers, The State University of New Jersey EXPANSION OF NATURAL KILLER CELLS AND CHIMERIC ANTIGEN RECEPTOR MODIFIED CELLS
AU2020232691B2 (en) 2019-03-05 2023-06-29 Nkarta, Inc. CD19-directed chimeric antigen receptors and uses thereof in immunotherapy
WO2020190771A1 (en) 2019-03-15 2020-09-24 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Chimeric adaptor and kinase signaling proteins and their use in immunotherapy
KR20200110576A (ko) * 2019-03-15 2020-09-24 재단법인대구경북과학기술원 사이토카인 기반 면역세포 및 그의 면역 치료 용도
EP3712257A1 (en) * 2019-03-21 2020-09-23 ONK Therapeutics Limited Modified natural killer cells with increased resistance to cell death
AU2020242305A1 (en) * 2019-03-21 2021-09-30 Onk Therapeutics Limited Modified immune effector cells with increased resistance to cell death
CN110079529A (zh) * 2019-04-28 2019-08-02 成都美杰赛尔生物科技有限公司 用于靶向敲除人NKG2A/KLRC1基因的sgRNA、表达载体、试剂盒及其用途
CN110106202A (zh) * 2019-05-07 2019-08-09 杭州师范大学 抗肿瘤nk细胞的制备方法及其细胞与应用
MX2021015448A (es) * 2019-06-14 2022-06-08 G Tech Bio Llc Células linfocíticas activadas y métodos para usarlas para tratar cáncer y afecciones infecciosas.
CN110205293A (zh) * 2019-06-25 2019-09-06 中冠赛尔生物科技(北京)有限公司 一种加强型高效治疗肺癌的nk免疫细胞的制备方法及应用
US20210077527A1 (en) * 2019-09-13 2021-03-18 The Research Institute At Nationwide Children's Hospital Universal donor selection method to identify nk-cell-donors
WO2021051088A1 (en) * 2019-09-13 2021-03-18 Ohio State Innovation Foundation Nk cell immunotherapy compositions, methods of making and methods of using same
US11230699B2 (en) 2020-01-28 2022-01-25 Immunitybio, Inc. Chimeric antigen receptor-modified NK-92 cells targeting EGFR super-family receptors
KR20220165254A (ko) 2020-03-26 2022-12-14 더 유나이티드 스테이츠 오브 어메리카, 애즈 리프리젠티드 바이 더 세크러테리, 디파트먼트 오브 헬쓰 앤드 휴먼 서비씨즈 Cd28h 도메인을 함유하는 키메라 항원 수용체 및 그 사용 방법
CN113528452B (zh) * 2020-07-06 2023-06-06 上海鑫湾生物科技有限公司 共表达IL-21和hrCD16嵌合受体的免疫细胞及其应用
CN116568805A (zh) * 2020-09-02 2023-08-08 恩卡尔塔公司 工程化自然杀伤细胞的增强扩增和细胞毒性及其用途
EP3964265A1 (en) * 2020-09-07 2022-03-09 Fundacion Instituto De Investigacion Sanitaria Fundacion Jimenez Diaz Mesenchymal stem cells co-expressing cxcr4 and il-10 and uses thereof
CN112029721A (zh) * 2020-09-09 2020-12-04 广东昭泰体内生物医药科技有限公司 一种活性增强型nk细胞的制备方法
KR102581230B1 (ko) * 2020-10-16 2023-09-21 의료법인 성광의료재단 항암 관련 유전자 발현이 조절된 자연살해세포 및 이의 용도
US20240009236A1 (en) 2020-11-04 2024-01-11 The United States Of America, As Represented By The Secretary, Dept. Of Health And Human Services Modified nk cells with reduced ccr5 expression and methods of their use
WO2022226130A1 (en) * 2021-04-21 2022-10-27 Indapta Therapeutics, Inc. Methods of treatment and dosing of natural killer cell compositions
CN115505572B (zh) * 2021-06-22 2025-11-14 康立泰生物医药(青岛)有限公司 一种增强型抗肿瘤nk细胞及其制备方法和应用
EP4395838A1 (en) * 2021-08-30 2024-07-10 CarryGenes Bioengineering, LLC tCD34 AND OTHER MARKERS FOR IDENTIFICATION AND SORTING OF CELLS AND FOR USE ASIN VIVO TRACKING ASSISTANT
WO2023044304A1 (en) 2021-09-15 2023-03-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Chimeric adaptor and kinase signaling proteins and their use in immunotherapy
CN114306606B (zh) * 2021-11-29 2023-05-26 江苏省人民医院（南京医科大学第一附属医院） cGAS抑制剂在制备治疗T细胞淋巴瘤的药物中的应用
CN114381434A (zh) * 2022-01-17 2022-04-22 华东师范大学 一种趋化型car-nk细胞及其制备方法和应用
CN114807237A (zh) * 2022-05-12 2022-07-29 广东普罗凯融生物医药科技有限公司 一种过表达CD16a的NK细胞的制备方法及其应用
CN115074325A (zh) * 2022-07-07 2022-09-20 广州希灵生物科技有限公司 Herceptin联合4-1BBL体外扩增NK方法
CN115466726B (zh) * 2022-09-05 2023-09-19 北京景达生物科技有限公司 一种nk细胞的高效基因转导方案
CN116178566A (zh) * 2022-09-30 2023-05-30 深圳博雅感知药业有限公司 靶向cd33的嵌合抗原受体和制备b16-cd33 car-nk细胞的方法
CN115975922B (zh) * 2022-12-15 2024-06-28 杭州师范大学 Bx795在制备nk-92细胞激活剂上的应用
WO2024131685A1 (zh) * 2022-12-19 2024-06-27 苏州沙砾生物科技有限公司 一种细胞培养方法及其用途
AU2024311300A1 (en) 2023-06-20 2026-01-15 Zelluna Immunotherapy As Methods of enhancing or modifying nk cells
WO2025232819A1 (en) * 2024-05-08 2025-11-13 Qihan Hong Kong Limited Immunotherapies using engineered cells for enhanced bone marrow homing
EP4692325A1 (en) * 2024-08-07 2026-02-11 Miltenyi Biotec B.V. & Co. KG Method for generation of a composition comprising natural killer cells

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020177551A1 (en) * 2000-05-31 2002-11-28 Terman David S. Compositions and methods for treatment of neoplastic disease
DE10019075B4 (de) * 2000-04-18 2007-01-18 Vision 7 Gmbh Verwendung von CD34 oder einem davon abgeleiteten Polypeptid als Zell-Oberflächen- bzw. Gentransfer-Marker
US20130266551A1 (en) * 2003-11-05 2013-10-10 St. Jude Children's Research Hospital, Inc. Chimeric receptors with 4-1bb stimulatory signaling domain
JP5525689B2 (ja) 2004-11-02 2014-06-18 ザ ガバメント オブ ザ ユナイテッド ステイツ オブ アメリカ アズ リプレゼンティッド バイ ザ セクレタリー デパートメント オブ ヘルス アンド ヒューマン サービシーズ 過剰増殖性障害を処置するための組成物および方法
CN101356679A (zh) * 2005-10-31 2009-01-28 昂考梅德药品有限公司 用于诊断和治疗癌症的组合物和方法
US20070264687A1 (en) * 2005-12-15 2007-11-15 Min-Yuan Chou Recombinant triplex scaffold-based polypeptides
CN101684456A (zh) * 2008-09-28 2010-03-31 江门罗森生物制药有限公司 一种体外培养条件下扩增人nk细胞的方法
DK2443242T3 (en) * 2009-06-17 2017-01-30 Tocagen Inc Production cells for replicative retroviral vectors
AU2013327136A1 (en) * 2012-10-02 2015-04-16 Memorial Sloan-Kettering Cancer Center Compositions and methods for immunotherapy
EP2793026A1 (en) * 2013-04-18 2014-10-22 Centre National de la Recherche Scientifique (CNRS) New method for monitoring cancer and/or inflammatory reaction based on relb phosphorylation
CN115028735A (zh) * 2013-05-03 2022-09-09 俄亥俄州创新基金会 Cs1-特异性嵌合抗原受体工程化的免疫效应细胞
US20170029777A1 (en) * 2014-01-27 2017-02-02 St. Jude Children's Research Hospital, Inc. Methods of expanding ex vivo natural killer t (nkt) cells and therapeutic uses thereof
WO2016073595A1 (en) 2014-11-05 2016-05-12 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services T cells and dendritic cells for polyomavirus therapy
US10813952B2 (en) 2014-11-14 2020-10-27 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services NK cells with an increased antibody-dependent cellular toxicity (ADCC) against tumors
WO2016085946A1 (en) * 2014-11-24 2016-06-02 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Active cxcr4+ immune cells and methods for their production and use
US12533338B2 (en) * 2015-09-30 2026-01-27 Vycellix, Inc. Enhanced gene delivery to natural killer cells, hematopoietic stem cells and macrophages
CN105567634A (zh) * 2016-01-27 2016-05-11 上海润泉生物技术有限公司 一种用于nk细胞体外扩增的培养基及nk细胞体外扩增的方法
US12516292B2 (en) * 2016-07-25 2026-01-06 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods of producing modified natural killer cells and methods of use
CN109844099B (zh) 2016-07-25 2024-01-02 美国政府(由卫生和人类服务部的部长所代表) 产生经修饰的自然杀伤细胞的方法及使用方法
CN106350487B (zh) * 2016-09-13 2019-01-25 北京多赢时代转化医学研究院 联合制备car-nk细胞和car-nkt细胞的方法
KR20240024328A (ko) * 2016-11-22 2024-02-23 알로플렉스 바이오테라퓨틱스 동종이형 종양 세포 백신

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