JP7086928B2 - 改変されたナチュラルキラー細胞を生成する方法および使用方法 - Google Patents

改変されたナチュラルキラー細胞を生成する方法および使用方法 Download PDF

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JP7086928B2
JP7086928B2 JP2019503538A JP2019503538A JP7086928B2 JP 7086928 B2 JP7086928 B2 JP 7086928B2 JP 2019503538 A JP2019503538 A JP 2019503538A JP 2019503538 A JP2019503538 A JP 2019503538A JP 7086928 B2 JP7086928 B2 JP 7086928B2
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リチャード ダブリュー. チャイルズ,
ダナラクシュミ チナサミー,
デイビッド エス.ジェイ. アラン,
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JP2019503538A 2016-07-25 2017-07-25 改変されたナチュラルキラー細胞を生成する方法および使用方法 Active JP7086928B2 (ja)

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US201662366493P 2016-07-25 2016-07-25
US62/366,493 2016-07-25
PCT/US2017/043774 WO2018022646A1 (en) 2016-07-25 2017-07-25 Methods of producing modified natural killer cells and methods of use

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EP (2) EP3487991B1 (https=)
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IL (1) IL273979B2 (https=)
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Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3143134T3 (da) 2014-05-15 2021-01-04 Nat Univ Singapore Modificerede, naturlige dræberceller og anvendelser deraf
US10813952B2 (en) * 2014-11-14 2020-10-27 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services NK cells with an increased antibody-dependent cellular toxicity (ADCC) against tumors
US12516292B2 (en) 2016-07-25 2026-01-06 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods of producing modified natural killer cells and methods of use
JP7086928B2 (ja) 2016-07-25 2022-06-20 ザ ユナイテッド ステイツ オブ アメリカ, アズ リプレゼンテッド バイ ザ セクレタリー, デパートメント オブ ヘルス アンド ヒューマン サービシーズ 改変されたナチュラルキラー細胞を生成する方法および使用方法
US20180344768A1 (en) * 2016-12-09 2018-12-06 Onkimmune Limited Nk cell-based therapy
CN110546265A (zh) 2017-02-09 2019-12-06 因达普塔治疗公司 工程化自然杀伤(nk)细胞及其组合物和方法
BR112019019917A2 (pt) 2017-03-27 2020-04-22 Nat Univ Singapore receptores quiméricos nkg2d truncados e seus usos em imunoterapia de células exterminadoras naturais
CA3056591A1 (en) 2017-03-27 2018-10-04 National University Of Singapore Stimulatory cell lines for ex vivo expansion and activation of natural killer cells
IL276365B2 (en) 2018-02-01 2023-10-01 Nkmax Co Ltd A method for the production of natural killer cells and a preparation for cancer treatment
EP3749685A4 (en) * 2018-02-09 2021-12-22 National University of Singapore ACTIVATOR CHEMERIC RECEPTORS AND THEIR USES IN NATURAL KILLING CELL IMMUNOTHERAPY
EP3755347B1 (en) * 2018-02-21 2025-06-25 Board of Regents, The University of Texas System Universal antigen presenting cells and uses thereof
US12473336B2 (en) 2018-02-21 2025-11-18 Board Of Regents, The University Of Texas System Methods for activation and expansion of natural killer cells and uses thereof
GB201804701D0 (en) * 2018-03-23 2018-05-09 Gammadelta Therapeutics Ltd Lymphocytes expressing heterologous targeting constructs
JP7334985B2 (ja) 2018-04-02 2023-08-29 ナショナル ユニヴァーシティー オブ シンガポール 免疫細胞で発現される膜結合抗サイトカイン非シグナル伝達バインダーによるヒトサイトカインの中和
US20210228630A1 (en) * 2018-05-16 2021-07-29 Research Institute At Nationwide Children's Hospital Generation of knock-out primary and expanded human nk cells using cas9 ribonucleoproteins
US12269863B2 (en) 2018-05-23 2025-04-08 Manysmart Therapeutics, Inc. Bispecific T cell engager and uses thereof
KR20210016567A (ko) 2018-05-30 2021-02-16 글리코스템 떼라퓨틱스 비.브이. Car nk 세포
WO2019232631A1 (en) * 2018-06-06 2019-12-12 Stemcell Technologies Canada Inc. Kits, compositions and methods for myeloid-derived suppressor cell enrichment
JP7241161B2 (ja) 2018-08-01 2023-03-16 イミュニティーバイオ、インコーポレイテッド 細胞免疫療法の安定した遺伝子改変のための、ホーミング受容体又はサイトカインとキメラ抗原受容体とを含むクアドリシストロニックシステム
SG11202102012SA (en) * 2018-08-28 2021-04-29 Pharos Vaccine Inc Improved lentiviral vector
US20210213119A1 (en) * 2018-08-28 2021-07-15 Immunotech Biopharm Co., Ltd. Improved therapeutic t cell
EP3844186A4 (en) 2018-08-29 2022-08-17 National University of Singapore METHOD OF SPECIFIC STIMULATION OF SURVIVAL AND EXPANSION OF GENETICALLY MODIFIED IMMUNE CELLS
CN110511912B (zh) * 2018-08-30 2024-03-22 浙江煦顼技术有限公司 免疫细胞的功能调节
WO2020077091A1 (en) * 2018-10-10 2020-04-16 Wisconsin Alumni Research Foundation Kir 7.1 gene therapy vectors and methods of using the same
CN109402053A (zh) * 2018-10-17 2019-03-01 广州元帅生物科技有限公司 一种外周血来源单个核细胞的分离及诱导培养方法
EP3884041A2 (en) * 2018-11-21 2021-09-29 Indapta Therapeutics, Inc. Methods for expansion of natural killer (nk) cell subset and related compositions and methods
CA3120563A1 (en) 2018-11-26 2020-06-04 Nkarta, Inc. Methods for the simultaneous expansion of multiple immune cell types, related compositions and uses of same in cancer immunotherapy
EP3927730A4 (en) 2019-02-20 2022-12-21 Rutgers, The State University of New Jersey EXPANSION OF NATURAL KILLER CELLS AND CHIMERIC ANTIGEN RECEPTOR MODIFIED CELLS
EP3773918A4 (en) 2019-03-05 2022-01-05 Nkarta, Inc. CD19 DIRECTED CHIMERIC ANTIGEN RECEPTORS AND THEIR USES IN IMMUNOTHERAPY
CN113710697A (zh) 2019-03-15 2021-11-26 美国政府(由卫生和人类服务部的部长所代表) 嵌合衔接子和激酶信号传导蛋白及其在免疫疗法中的用途
KR20200110576A (ko) * 2019-03-15 2020-09-24 재단법인대구경북과학기술원 사이토카인 기반 면역세포 및 그의 면역 치료 용도
JP2022526504A (ja) * 2019-03-21 2022-05-25 オーエヌケー セラピューティクス リミテッド 細胞死に対する耐性が増大した改変免疫エフェクター細胞
EP3712257A1 (en) * 2019-03-21 2020-09-23 ONK Therapeutics Limited Modified natural killer cells with increased resistance to cell death
CN110079529A (zh) * 2019-04-28 2019-08-02 成都美杰赛尔生物科技有限公司 用于靶向敲除人NKG2A/KLRC1基因的sgRNA、表达载体、试剂盒及其用途
CN110106202A (zh) * 2019-05-07 2019-08-09 杭州师范大学 抗肿瘤nk细胞的制备方法及其细胞与应用
AU2020290969A1 (en) * 2019-06-14 2022-02-03 G Tech Bio Llc Activated lymphocytic cells and methods of using the same to treat cancer and infectious conditions
CN110205293A (zh) * 2019-06-25 2019-09-06 中冠赛尔生物科技(北京)有限公司 一种加强型高效治疗肺癌的nk免疫细胞的制备方法及应用
US20220347216A1 (en) * 2019-09-13 2022-11-03 Ohio State Innovation Foundation Nk cell immunotherapy compositions, methods of making and methods of using same
US20210077527A1 (en) * 2019-09-13 2021-03-18 The Research Institute At Nationwide Children's Hospital Universal donor selection method to identify nk-cell-donors
US11230699B2 (en) 2020-01-28 2022-01-25 Immunitybio, Inc. Chimeric antigen receptor-modified NK-92 cells targeting EGFR super-family receptors
BR112022017512A2 (pt) 2020-03-26 2022-10-18 Us Health Receptores de antígeno quiméricos contendo domínio cd28h e métodos de uso dos mesmo
WO2022007796A1 (zh) * 2020-07-06 2022-01-13 上海鑫湾生物科技有限公司 共表达IL-21和hrCD16嵌合受体的免疫细胞及其应用
IL300903A (en) * 2020-09-02 2023-04-01 Nkarta Inc Expansion and enhanced cytotoxicity of natural killer cells and their uses
EP3964265A1 (en) * 2020-09-07 2022-03-09 Fundacion Instituto De Investigacion Sanitaria Fundacion Jimenez Diaz Mesenchymal stem cells co-expressing cxcr4 and il-10 and uses thereof
CN112029721A (zh) * 2020-09-09 2020-12-04 广东昭泰体内生物医药科技有限公司 一种活性增强型nk细胞的制备方法
KR102581230B1 (ko) * 2020-10-16 2023-09-21 의료법인 성광의료재단 항암 관련 유전자 발현이 조절된 자연살해세포 및 이의 용도
WO2022098718A1 (en) 2020-11-04 2022-05-12 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Modified nk cells with reduced ccr5 expression and methods of their use
US20240197783A1 (en) * 2021-04-21 2024-06-20 Indapta Therapeutics, Inc. Methods of treatment and dosing of natural killer cell compositions
CN115505572B (zh) * 2021-06-22 2025-11-14 康立泰生物医药(青岛)有限公司 一种增强型抗肿瘤nk细胞及其制备方法和应用
AU2022337186A1 (en) * 2021-08-30 2025-03-06 Carrygenes Bioengineering, Llc Use of chemokine receptors in cellular homing
WO2023044304A1 (en) 2021-09-15 2023-03-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Chimeric adaptor and kinase signaling proteins and their use in immunotherapy
CN114306606B (zh) * 2021-11-29 2023-05-26 江苏省人民医院(南京医科大学第一附属医院) cGAS抑制剂在制备治疗T细胞淋巴瘤的药物中的应用
CN114381434A (zh) * 2022-01-17 2022-04-22 华东师范大学 一种趋化型car-nk细胞及其制备方法和应用
CN114807237A (zh) * 2022-05-12 2022-07-29 广东普罗凯融生物医药科技有限公司 一种过表达CD16a的NK细胞的制备方法及其应用
CN115074325A (zh) * 2022-07-07 2022-09-20 广州希灵生物科技有限公司 Herceptin联合4-1BBL体外扩增NK方法
CN115466726B (zh) * 2022-09-05 2023-09-19 北京景达生物科技有限公司 一种nk细胞的高效基因转导方案
CN116178566A (zh) * 2022-09-30 2023-05-30 深圳博雅感知药业有限公司 靶向cd33的嵌合抗原受体和制备b16-cd33 car-nk细胞的方法
CN115975922B (zh) * 2022-12-15 2024-06-28 杭州师范大学 Bx795在制备nk-92细胞激活剂上的应用
CN120344655A (zh) * 2022-12-19 2025-07-18 苏州沙砾生物科技有限公司 一种细胞培养方法及其用途
WO2024261045A1 (en) 2023-06-20 2024-12-26 Zelluna Immunotherapy As Methods of enhancing or modifying nk cells
WO2025232819A1 (en) * 2024-05-08 2025-11-13 Qihan Hong Kong Limited Immunotherapies using engineered cells for enhanced bone marrow homing
EP4692325A1 (en) * 2024-08-07 2026-02-11 Miltenyi Biotec B.V. & Co. KG Method for generation of a composition comprising natural killer cells

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020177551A1 (en) * 2000-05-31 2002-11-28 Terman David S. Compositions and methods for treatment of neoplastic disease
DE10019075B4 (de) * 2000-04-18 2007-01-18 Vision 7 Gmbh Verwendung von CD34 oder einem davon abgeleiteten Polypeptid als Zell-Oberflächen- bzw. Gentransfer-Marker
US20130266551A1 (en) * 2003-11-05 2013-10-10 St. Jude Children's Research Hospital, Inc. Chimeric receptors with 4-1bb stimulatory signaling domain
JP5525689B2 (ja) 2004-11-02 2014-06-18 ザ ガバメント オブ ザ ユナイテッド ステイツ オブ アメリカ アズ リプレゼンティッド バイ ザ セクレタリー デパートメント オブ ヘルス アンド ヒューマン サービシーズ 過剰増殖性障害を処置するための組成物および方法
CN101356679A (zh) * 2005-10-31 2009-01-28 昂考梅德药品有限公司 用于诊断和治疗癌症的组合物和方法
US20070264687A1 (en) * 2005-12-15 2007-11-15 Min-Yuan Chou Recombinant triplex scaffold-based polypeptides
CN101684456A (zh) * 2008-09-28 2010-03-31 江门罗森生物制药有限公司 一种体外培养条件下扩增人nk细胞的方法
JP5923037B2 (ja) * 2009-06-17 2016-05-24 トカジェン インコーポレーテッド 複製可能レトロウイルスベクターの産生細胞
MX370148B (es) * 2012-10-02 2019-12-03 Memorial Sloan Kettering Cancer Center Composiciones y su uso para inmunoterapia.
EP2793026A1 (en) * 2013-04-18 2014-10-22 Centre National de la Recherche Scientifique (CNRS) New method for monitoring cancer and/or inflammatory reaction based on relb phosphorylation
CN105377897A (zh) * 2013-05-03 2016-03-02 俄亥俄州创新基金会 Cs1-特异性嵌合抗原受体工程化的免疫效应细胞
WO2015112793A2 (en) * 2014-01-27 2015-07-30 St. Jude Children's Research Hospital, Inc. Methods of expanding ex vivo natural killer t (nkt) cells and therapeutic uses thereof
WO2016073595A1 (en) 2014-11-05 2016-05-12 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services T cells and dendritic cells for polyomavirus therapy
US10813952B2 (en) 2014-11-14 2020-10-27 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services NK cells with an increased antibody-dependent cellular toxicity (ADCC) against tumors
US10815457B2 (en) * 2014-11-24 2020-10-27 University Of Pittsburgh Of The Commonwealth Systems Of Higher Education Active CXCR4+ immune cells and methods for their production and use
JP7034065B2 (ja) * 2015-09-30 2022-03-11 ヴィセリックス・インコーポレーティッド ナチュラルキラー細胞、造血幹細胞及びマクロファージへの強化された遺伝子送達
CN105567634A (zh) * 2016-01-27 2016-05-11 上海润泉生物技术有限公司 一种用于nk细胞体外扩增的培养基及nk细胞体外扩增的方法
US12516292B2 (en) * 2016-07-25 2026-01-06 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods of producing modified natural killer cells and methods of use
JP7086928B2 (ja) 2016-07-25 2022-06-20 ザ ユナイテッド ステイツ オブ アメリカ, アズ リプレゼンテッド バイ ザ セクレタリー, デパートメント オブ ヘルス アンド ヒューマン サービシーズ 改変されたナチュラルキラー細胞を生成する方法および使用方法
CN106350487B (zh) * 2016-09-13 2019-01-25 北京多赢时代转化医学研究院 联合制备car-nk细胞和car-nkt细胞的方法
KR20240024328A (ko) * 2016-11-22 2024-02-23 알로플렉스 바이오테라퓨틱스 동종이형 종양 세포 백신

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Cancer Immunol Immunother, 2010, vol.59,pp.851-862
Frontiers in Immunology, 2016, vol.7,Article 152
Hematology, 2013, Vol.1, pp.234-246
Molecular Therapy, 2008, vol.16,Supplement 1, pp.S286, S287

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