SG10201913687VA - Generation of midbrain-specific organoids from human pluripotent stem cells - Google Patents
Generation of midbrain-specific organoids from human pluripotent stem cellsInfo
- Publication number
- SG10201913687VA SG10201913687VA SG10201913687VA SG10201913687VA SG10201913687VA SG 10201913687V A SG10201913687V A SG 10201913687VA SG 10201913687V A SG10201913687V A SG 10201913687VA SG 10201913687V A SG10201913687V A SG 10201913687VA SG 10201913687V A SG10201913687V A SG 10201913687VA
- Authority
- SG
- Singapore
- Prior art keywords
- midbrain
- generation
- stem cells
- pluripotent stem
- human pluripotent
- Prior art date
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0062—General methods for three-dimensional culture
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- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
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- C12N5/0619—Neurons
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
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- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
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- Biotechnology (AREA)
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- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Neurology (AREA)
- Neurosurgery (AREA)
- Developmental Biology & Embryology (AREA)
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Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SG10201601965P | 2016-03-14 |
Publications (1)
Publication Number | Publication Date |
---|---|
SG10201913687VA true SG10201913687VA (en) | 2020-03-30 |
Family
ID=59851957
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201807487WA SG11201807487WA (en) | 2016-03-14 | 2017-03-14 | Generation of midbrain-specific organoids from human pluripotent stem cells |
SG10201913687VA SG10201913687VA (en) | 2016-03-14 | 2017-03-14 | Generation of midbrain-specific organoids from human pluripotent stem cells |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201807487WA SG11201807487WA (en) | 2016-03-14 | 2017-03-14 | Generation of midbrain-specific organoids from human pluripotent stem cells |
Country Status (5)
Country | Link |
---|---|
US (1) | US20190169576A1 (fr) |
EP (1) | EP3430132A4 (fr) |
CN (1) | CN109996870A (fr) |
SG (2) | SG11201807487WA (fr) |
WO (1) | WO2017160234A1 (fr) |
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US9719068B2 (en) | 2010-05-06 | 2017-08-01 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into intestinal tissues through directed differentiation |
CA2949834A1 (fr) | 2014-05-28 | 2015-12-03 | James Macormack Wells | Procedes et systemes de conversion de cellules precurseurs en tissus gastriques par differenciation dirigee |
JP6804438B2 (ja) | 2014-10-17 | 2020-12-23 | チルドレンズ ホスピタル メディカル センター | 多能性幹細胞を使用するヒト小腸のin vivoモデル、並びにそれを作製、及び使用する方法 |
CA3016641A1 (fr) | 2016-05-05 | 2017-11-09 | Children's Hospital Medical Center | Procedes de fabrication in vitro de tissu de fundus d'estomac et compositions associees a celui-ci |
US10583286B2 (en) * | 2016-08-23 | 2020-03-10 | Boston Scientific Neuromodulation Corporation | Methods and devices for neuromodulation of the adrenal gland |
CN110062764A (zh) | 2016-12-05 | 2019-07-26 | 儿童医院医学中心 | 结肠类器官及其制备和使用方法 |
CN108456659B (zh) * | 2018-03-14 | 2020-05-05 | 浙江霍德生物工程有限公司 | 3d大脑类器官的制备方法 |
CN110295145B (zh) * | 2018-03-22 | 2020-10-16 | 苏州麦迪耐斯医药科技有限公司 | 一种体外培养脑肿瘤细胞的培养液及培养方法 |
CA3111582A1 (fr) * | 2018-09-11 | 2020-03-19 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Generation et analyse automatisees d'organoides |
CN112996906A (zh) * | 2018-11-15 | 2021-06-18 | Jsr株式会社 | 脑类器官的制造方法 |
EP3924468A1 (fr) * | 2019-02-11 | 2021-12-22 | Miltenyi Biotec B.V. & Co. KG | Génération de structures de tissu artificiel dérivées de cellules souches pluripotentes humaines sans matrices tridimensionnelles |
JP7366332B2 (ja) * | 2019-06-05 | 2023-10-23 | 公立大学法人奈良県立医科大学 | 脳幹オルガノイドの作製方法 |
CN114026222A (zh) * | 2019-07-05 | 2022-02-08 | 学校法人庆应义塾 | 脑类器官的制造方法 |
KR102228400B1 (ko) * | 2019-09-24 | 2021-03-16 | 고려대학교 산학협력단 | 뇌 오가노이드 제조 방법 |
US20220389378A1 (en) * | 2019-11-22 | 2022-12-08 | Novo Nordisk A/S | Spin-aggregated neural microspheres and the application thereof |
IL294565A (en) * | 2020-01-13 | 2022-09-01 | Aspen Neuroscience Inc | Method for Differentiating Neurons and Related Compositions and Methods of Use |
US20230165909A1 (en) * | 2020-04-21 | 2023-06-01 | Aspen Neuroscience, Inc. | Gene editing of lrrk2 in stem cells and method of use of cells differentiated therefrom |
WO2021216622A1 (fr) * | 2020-04-21 | 2021-10-28 | Aspen Neuroscience, Inc. | Édition génique de gba1 dans des cellules souches et procédé d'utilisation de cellules différenciées à partir de celles-ci |
WO2021232234A1 (fr) * | 2020-05-19 | 2021-11-25 | 武汉睿健医药科技有限公司 | Procédé d'induction sans sérum pour cellule neuronale sensorielle |
WO2021251820A1 (fr) * | 2020-06-08 | 2021-12-16 | Technische Universiteit Eindhoven | Procédés de production d'un tissu neuronal (tridimensionnel) |
WO2022005023A1 (fr) * | 2020-06-29 | 2022-01-06 | 주식회사 오간팩토리 | Organoïde de mésencéphale, son procédé de production rapide en grandes quantités, et procédé de criblage de neurotoxines et procédé de criblage de médicaments pour une maladie associée aux neurones dopaminergiques l'utilisant |
KR102346243B1 (ko) * | 2020-06-29 | 2022-01-04 | 주식회사 오간팩토리 | 중뇌 오가노이드, 이의 고속 및 대량 제조 방법, 이를 이용한 신경독성물질 스크리닝 방법 및 도파민성 신경세포 관련 질환 치료제 스크리닝 방법 |
EP3992280A1 (fr) * | 2020-10-30 | 2022-05-04 | Kugelmeiers Ltd. | Procédé de génération de neurosphères |
CN114736864B (zh) * | 2020-12-23 | 2024-01-26 | 武汉睿健医药科技有限公司 | 一种神经干细胞诱导分化培养基及诱导分化方法 |
CN113046309B (zh) * | 2021-03-26 | 2023-05-05 | 成都云测医学生物技术有限公司 | 悬浮培养脑类器官的培养基及其应用 |
CN113249319B (zh) * | 2021-05-19 | 2022-11-11 | 北京大学 | 一种脑脊液类器官的培养基及其培养方法 |
CN113789301B (zh) * | 2021-08-27 | 2022-05-10 | 广州瑞臻再生医学科技有限公司 | 一种制备高纯度的诱导性多能干细胞来源的人脑多巴胺能神经元的方法 |
CN113493768B (zh) * | 2021-09-06 | 2021-12-24 | 中国科学院动物研究所 | 神经类器官及其制备方法 |
KR20230081737A (ko) * | 2021-11-29 | 2023-06-08 | 주식회사 오간팩토리 | 간 오가노이드, 이의 고속 및 대량 제조 방법, 이를 이용한 약물의 효능 및 독성 스크리닝 방법 |
CN115404218A (zh) * | 2022-01-13 | 2022-11-29 | 南华大学附属第一医院 | 一种包含胶质细胞的3d人脑类器官培养方法 |
WO2023137200A2 (fr) * | 2022-01-13 | 2023-07-20 | The Board Of Trustees Of The Leland Stanford Junior University | Génération d'organoïdes d'organisateur neuronal et d'assembloïdes de ligne médiane à partir de cellules souches pluripotentes humaines |
WO2023201046A1 (fr) * | 2022-04-14 | 2023-10-19 | Lieber Institute, Inc. | Compositions et méthode de traitement d'une dystonie-parkinsonisme liée à l'x |
EP4286513A1 (fr) | 2022-06-03 | 2023-12-06 | IMBA-Institut für Molekulare Biotechnologie GmbH | Fusion de culture de tissus triple |
WO2024017538A1 (fr) * | 2022-07-19 | 2024-01-25 | Medizinische Hochschule Hannover | Procédé de production d'organoïdes à partir de cellules de mammifère |
EP4310177A1 (fr) * | 2022-07-19 | 2024-01-24 | Medizinische Hochschule Hannover | Procédé de production d'organoïdes à partir de cellules de mammifères |
CN115044539A (zh) * | 2022-08-16 | 2022-09-13 | 中义(北京)健康研究院 | 一种鲨鱼肝细胞及其培养方法 |
CN117778313B (zh) * | 2024-02-23 | 2024-05-24 | 成都云测医学生物技术有限公司 | 脑类器官获得间充质干细胞分化方法和应用 |
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Publication number | Priority date | Publication date | Assignee | Title |
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GB201111244D0 (en) * | 2011-06-30 | 2011-08-17 | Konink Nl Akademie Van Wetenschappen Knaw | Culture media for stem cells |
MX368315B (es) * | 2012-04-24 | 2019-09-27 | Int Stem Cell Corporation | Derivación de células madre neurales y neuronas dopaminérgicas a partir de células madre pluripotentes humanas. |
EP2743345A1 (fr) * | 2012-12-13 | 2014-06-18 | IMBA-Institut für Molekulare Biotechnologie GmbH | Culture de tissu différencié hétérogène tridimensionnel |
KR101696874B1 (ko) * | 2013-07-31 | 2017-01-16 | 한국생명공학연구원 | 직접 리프로그래밍을 통한 유도 도파민성 전구세포 제조방법 |
DE102014003465A1 (de) * | 2014-03-11 | 2015-09-17 | NeuroProof GmbH | Gewinnung von Gehirnregion-spezifischen neuronalen Kulturen aus dreidimensionalen Gewebekulturen von Stammzellen |
DK3119881T3 (da) * | 2014-03-21 | 2023-04-03 | Fujifilm Cellular Dynamics Inc | Production of midbrain dopaminergic neurons and methods for the use thereof |
CN107427537A (zh) * | 2015-03-03 | 2017-12-01 | 哈佛学院院长及董事 | 产生功能性人体组织的方法 |
LU92845B1 (en) * | 2015-10-08 | 2017-05-02 | Univ Du Luxembourg Campus Belval | Means and methods for generating midbrain organoids |
EP3190176A1 (fr) * | 2016-01-11 | 2017-07-12 | IMBA-Institut für Molekulare Biotechnologie GmbH | Procédé de développement de culture tissulaire sur échafaudage et culture de tissus différenciés |
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2017
- 2017-03-14 EP EP17767082.5A patent/EP3430132A4/fr active Pending
- 2017-03-14 SG SG11201807487WA patent/SG11201807487WA/en unknown
- 2017-03-14 US US16/085,553 patent/US20190169576A1/en active Pending
- 2017-03-14 SG SG10201913687VA patent/SG10201913687VA/en unknown
- 2017-03-14 CN CN201780029142.2A patent/CN109996870A/zh active Pending
- 2017-03-14 WO PCT/SG2017/050126 patent/WO2017160234A1/fr active Application Filing
Also Published As
Publication number | Publication date |
---|---|
SG11201807487WA (en) | 2018-09-27 |
CN109996870A (zh) | 2019-07-09 |
EP3430132A1 (fr) | 2019-01-23 |
US20190169576A1 (en) | 2019-06-06 |
EP3430132A4 (fr) | 2019-12-25 |
WO2017160234A1 (fr) | 2017-09-21 |
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