SG11201705695QA - Differentiation of macrophages from pluripotent stem cells - Google Patents

Differentiation of macrophages from pluripotent stem cells

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Publication number
SG11201705695QA
SG11201705695QA SG11201705695QA SG11201705695QA SG11201705695QA SG 11201705695Q A SG11201705695Q A SG 11201705695QA SG 11201705695Q A SG11201705695Q A SG 11201705695QA SG 11201705695Q A SG11201705695Q A SG 11201705695QA SG 11201705695Q A SG11201705695Q A SG 11201705695QA
Authority
SG
Singapore
Prior art keywords
macrophages
differentiation
stem cells
pluripotent stem
pluripotent
Prior art date
Application number
SG11201705695QA
Inventor
Hwei-In Shawn Lim
Tara Huber
Florent Ginhoux
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Agency Science Tech & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Publication of SG11201705695QA publication Critical patent/SG11201705695QA/en

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    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0645—Macrophages, e.g. Kuepfer cells in the liver; Monocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/15—Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cells; Myeloid precursor cells; Antigen-presenting cells, e.g. dendritic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/461—Cellular immunotherapy characterised by the cell type used
    • A61K39/4614—Monocytes; Macrophages
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/462—Cellular immunotherapy characterized by the effect or the function of the cells
    • A61K39/4621—Cellular immunotherapy characterized by the effect or the function of the cells immunosuppressive or immunotolerising
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/462—Cellular immunotherapy characterized by the effect or the function of the cells
    • A61K39/4622—Antigen presenting cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643—Vertebrate antigens
    • A61K39/46433—Antigens related to auto-immune diseases; Preparations to induce self-tolerance
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/5055—Cells of the immune system involving macrophages
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5058—Neurological cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K39/46
    • A61K2239/31—Indexing codes associated with cellular immunotherapy of group A61K39/46 characterized by the route of administration
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    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2303—Interleukin-3 (IL-3)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/2306—Interleukin-6 (IL-6)
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    • C12N2501/415—Wnt; Frizzeled
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
SG11201705695QA 2015-01-16 2016-01-18 Differentiation of macrophages from pluripotent stem cells SG11201705695QA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SG10201500366V 2015-01-16
PCT/SG2016/050018 WO2016114723A1 (en) 2015-01-16 2016-01-18 Differentiation of macrophages from pluripotent stem cells

Publications (1)

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SG11201705695QA true SG11201705695QA (en) 2017-08-30

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US (2) US10724003B2 (en)
EP (1) EP3245289B1 (en)
JP (1) JP6774952B2 (en)
CN (1) CN107208062B (en)
SG (1) SG11201705695QA (en)
WO (1) WO2016114723A1 (en)

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WO2014100779A1 (en) * 2012-12-21 2014-06-26 Advanced Cell Technology, Inc. Methods ofr production of platelets from pluripotent stem cells and compositions thereof
EP3245289B1 (en) * 2015-01-16 2021-07-07 Agency For Science, Technology And Research Differentiation of macrophages from pluripotent stem cells
US11285174B2 (en) 2016-09-19 2022-03-29 The University Court Of The University Of Edinburgh Macrophage-based therapy for use in the treatment of liver injury
EP3399027A1 (en) 2017-05-04 2018-11-07 Medizinische Hochschule Hannover Stem-cell derived myeloid cells, generation and use thereof
US11629331B2 (en) 2017-07-19 2023-04-18 Seoul National University R & Db Foundation Method of differentiation of human induced pluripotent stem cell to dermal papilla precursor cell and use thereof
WO2019017691A2 (en) * 2017-07-19 2019-01-24 서울대학교 산학협력단 Method of differentiation of human induced pluripotent stem cell to dermal papilla precursor cell and use thereof
CN107807234A (en) * 2017-10-30 2018-03-16 柏基香 A kind of method for detecting intracerebral microglia phagocytic activity
CN107630000B (en) * 2017-11-06 2021-07-13 中国农业大学 Kit for separating and culturing peripheral blood-derived macrophages of livestock
WO2019117444A1 (en) * 2017-12-11 2019-06-20 대한민국(관리부서 질병관리본부장) Differentiation of immunocytes using pluripotent stem cells
EP3740566A4 (en) * 2018-01-18 2021-10-27 Agency for Science, Technology and Research Method for differentiation of human pluripotent stem cell lines in suspension culture
CN108300695B (en) * 2018-02-07 2021-08-06 清华大学 Method for differentiating human pluripotent stem cells into hematopoietic stem cells and culture additive
CN109082411B (en) * 2018-09-07 2022-06-07 赛元生物科技(杭州)有限公司 Method for obtaining phagocytic macrophage by differentiation of pluripotent stem cells
CA3109546A1 (en) * 2018-09-07 2020-03-12 Wisconsin Alumni Research Foundation Generation of hematopoietic progenitor cells from human pluripotent stem cells
CN113166219A (en) * 2018-09-28 2021-07-23 纪念斯隆-凯特琳癌症中心 Stem cell derived human microglia, methods of making, and methods of use
CN109266618B (en) * 2018-10-18 2021-04-23 赛元生物科技(杭州)有限公司 Macrophage capable of targeting tumor cells and preparation method thereof
CN113924362A (en) * 2019-03-29 2022-01-11 新加坡科技研究局 Microglial-rich brain organoids
CA3227202A1 (en) * 2021-08-19 2023-02-23 Cedars-Sinai Medical Center Ipsc-derived immune cells in prophylaxis and treatment of age-associated and neurodegenerative diseases
WO2024034656A1 (en) * 2022-08-09 2024-02-15 Ａｇｃ株式会社 METHOD FOR PRODUCING PROLIFERATIVE MACROPHAGE-LIKE CELLS (pMAC)
CN115433715B (en) * 2022-08-15 2023-04-14 呈诺再生医学科技(北京)有限公司 Culture medium and method for inducing iPSC differentiation to obtain macrophages and application of culture medium
WO2024054769A1 (en) * 2022-09-07 2024-03-14 Deverra Therapeutics Inc. Methods for the generation of a monocyte/macrophage cell product from expanded cord blood cd34+ hematopoietic stem and progenitor cells
CN116731967B (en) * 2023-08-16 2023-11-17 南京大学 Method for preparing macrophage from multipotent stem cells by induced differentiation
CN116987667B (en) * 2023-09-26 2024-01-09 天海元祺生物科技(天津)有限公司 Method for differentiating pluripotent stem cells into macrophages or neutrophils

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EP1463803B1 (en) * 2001-12-07 2018-02-14 Asterias Biotherapeutics, Inc. Hematopoietic cells from human embryonic stem cells
JP2012517822A (en) * 2009-02-19 2012-08-09 ガラパゴス・ナムローゼ・フェンノートシャップ Identification method and compound useful for diagnosis and treatment of diseases including inflammation
AU2010217739B2 (en) 2009-02-27 2015-09-03 FUJIFILM Cellular Dynamics, Inc. Differentiation of pluripotent cells
EP2598882B1 (en) * 2010-07-30 2017-07-26 AC Immune S.A. Safe and functional humanized antibodies for use in treating an amyloidosis
WO2014012933A1 (en) * 2012-07-16 2014-01-23 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Method for the generation of non-transformed macrophage cell lines
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US10081792B2 (en) * 2014-12-31 2018-09-25 Wisconsin Alumni Research Foundation Derivation of human microglia from pluripotent stem cells
EP3245289B1 (en) * 2015-01-16 2021-07-07 Agency For Science, Technology And Research Differentiation of macrophages from pluripotent stem cells
CA2975780C (en) * 2015-02-11 2023-08-01 The Hospital For Sick Children Alveolar-like macrophages and method of generating same

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JP6774952B2 (en) 2020-10-28
JP2018503375A (en) 2018-02-08
US20180010096A1 (en) 2018-01-11
US11618886B2 (en) 2023-04-04
EP3245289B1 (en) 2021-07-07
CN107208062A (en) 2017-09-26
WO2016114723A1 (en) 2016-07-21
CN107208062B (en) 2021-03-09
US20200399601A1 (en) 2020-12-24
EP3245289A4 (en) 2018-06-27
EP3245289A1 (en) 2017-11-22
US10724003B2 (en) 2020-07-28

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