SG11201708359TA - Generation of functional cells from stem cells - Google Patents

Generation of functional cells from stem cells

Info

Publication number
SG11201708359TA
SG11201708359TA SG11201708359TA SG11201708359TA SG11201708359TA SG 11201708359T A SG11201708359T A SG 11201708359TA SG 11201708359T A SG11201708359T A SG 11201708359TA SG 11201708359T A SG11201708359T A SG 11201708359TA SG 11201708359T A SG11201708359T A SG 11201708359TA
Authority
SG
Singapore
Prior art keywords
cells
generation
functional
stem cells
stem
Prior art date
Application number
SG11201708359TA
Inventor
Xuyang Sun
Bing Lim
Original Assignee
Agency Science Tech & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Publication of SG11201708359TA publication Critical patent/SG11201708359TA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/65—MicroRNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/08—Coculture with; Conditioned medium produced by cells of the nervous system
    • C12N2502/086—Coculture with; Conditioned medium produced by cells of the nervous system glial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
SG11201708359TA 2015-04-10 2016-04-11 Generation of functional cells from stem cells SG11201708359TA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SG10201502869T 2015-04-10
PCT/SG2016/050176 WO2016163958A1 (en) 2015-04-10 2016-04-11 Generation of functional cells from stem cells

Publications (1)

Publication Number Publication Date
SG11201708359TA true SG11201708359TA (en) 2017-11-29

Family

ID=57071959

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201708359TA SG11201708359TA (en) 2015-04-10 2016-04-11 Generation of functional cells from stem cells

Country Status (6)

Country Link
US (1) US20180072988A1 (en)
EP (1) EP3280797A4 (en)
JP (1) JP2018513686A (en)
CN (1) CN107709549A (en)
SG (1) SG11201708359TA (en)
WO (1) WO2016163958A1 (en)

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CN109689860A (en) 2016-09-12 2019-04-26 哈佛学院院长及董事 The differentiation of transcription factor control stem cell
GB201619876D0 (en) 2016-11-24 2017-01-11 Cambridge Entpr Ltd Controllable transcription
JPWO2018155620A1 (en) * 2017-02-24 2020-01-09 剛士 田邊 Method for producing neural cells
JP2018143239A (en) * 2017-03-01 2018-09-20 エリクサジェン，エルエルシー． Methods for efficient differentiation of pluripotent stem cells into desired cell types
US20200377885A1 (en) * 2017-08-07 2020-12-03 Washington University Compositions and methods for the generation of neurons and uses thereof
EP3719133A4 (en) * 2017-11-30 2021-08-11 I Peace, Inc. Method for producing neural cells
WO2019108894A1 (en) * 2017-12-01 2019-06-06 President And Fellows Of Harvard College Methods and compositions for the production of oligodendrocyte progenitor cells
US11788131B2 (en) 2018-04-06 2023-10-17 President And Fellows Of Harvard College Methods of identifying combinations of transcription factors
US20220235104A1 (en) * 2018-07-06 2022-07-28 The Regents Of The University Of California Novel method to engineer translantable human tissues
CN108998453A (en) * 2018-07-18 2018-12-14 暨南大学 It is a kind of target OC-2 gene siRNA and its application
CN110857436B (en) * 2018-08-24 2021-06-29 中山大学中山眼科中心 Method for preparing retinal ganglion cells by in vitro reprogramming
IT201800009133A1 (en) * 2018-10-03 2020-04-03 Università Cattolica del Sacro Cuore IDENTIFICATION OF PREDICTIVE RESPONSE MOLECULAR BIOMARCATORS TO RADIOCHEMOTHERAPY TREATMENT IN CERVICAL CARCINOMA
AU2019368519B2 (en) * 2018-10-26 2022-11-03 Shandong University System and method for real-time screening and measurement of cellular specific photosensitive effect
US20220169990A1 (en) * 2019-04-09 2022-06-02 I Peace, Inc. Method for producing cardiomyocyte
KR102183208B1 (en) * 2019-07-25 2020-11-25 한국과학기술연구원 Methods for reprogramming astrocytes into neurons in spinal cord injury(SCI) animal model using Ngn2
WO2021039965A1 (en) * 2019-08-29 2021-03-04 学校法人慶應義塾 Method for producing parvalbumin-positive nerve cells, cell, and differentiation inducer
CN112704567A (en) * 2019-10-25 2021-04-27 复旦大学附属华山医院 Experimental method for determining anatomical functional boundary of lateral Subnucleus (SLD) of animal pons dorsum
KR102249776B1 (en) * 2019-11-25 2021-05-10 연세대학교 산학협력단 Improved reprogramming system in vivo and a method for cell conversion using thereof
US11788065B2 (en) 2019-12-23 2023-10-17 Boston Medical Center Corporation Human iPSC-based derivation of NK and T-cells using early Notch induction
CA3175071A1 (en) 2020-03-11 2021-09-16 Bit Bio Limited Method of generating hepatic cells
KR102534488B1 (en) * 2020-09-10 2023-05-22 고려대학교 산학협력단 Efficient and short-term method for generating functional astrocytes
WO2022055239A1 (en) * 2020-09-10 2022-03-17 고려대학교 산학협력단 Method for quickly and efficiently differentiating functional glial cells
CN111961650B (en) * 2020-10-20 2023-06-13 士泽生物医药（苏州）有限公司 Method for obtaining glial cells in vitro and application thereof
CN112305209B (en) * 2020-10-26 2021-11-23 南开大学 Non-contact adherent cell three-dimensional morphology measuring method and cell sealing method
WO2023191425A1 (en) * 2022-03-28 2023-10-05 주식회사 비엔에이치리서치 Electrophysiological screening method for drugs
WO2023192939A2 (en) * 2022-04-01 2023-10-05 President And Fellows Of Harvard College Methods and compositions for producing oogonia-like cells

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Publication number Priority date Publication date Assignee Title
US6602680B2 (en) * 2000-07-14 2003-08-05 The Regents Of The University Of California Production of gabaergic cells
US20070087437A1 (en) * 2005-10-14 2007-04-19 Jifan Hu Methods for rejuvenating cells in vitro and in vivo
US9365821B2 (en) * 2009-09-01 2016-06-14 Mcmaster University Transformed human pluripotent stem cells and associated methods
CA2796251C (en) * 2010-04-13 2019-05-14 Cellular Dynamics International, Inc. Hepatocyte production by forward programming
KR20140121317A (en) * 2013-04-06 2014-10-15 서울대학교산학협력단 Method for producing induced neural stem cell from non-neuronal cell, and induced neural stem cell produced by the same

Also Published As

Publication number Publication date
CN107709549A (en) 2018-02-16
EP3280797A1 (en) 2018-02-14
JP2018513686A (en) 2018-05-31
WO2016163958A1 (en) 2016-10-13
US20180072988A1 (en) 2018-03-15
EP3280797A4 (en) 2019-01-16

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