SE459816B - Vektor med stark genexpression - Google Patents

Vektor med stark genexpression

Info

Publication number
SE459816B
SE459816B SE8303068A SE8303068A SE459816B SE 459816 B SE459816 B SE 459816B SE 8303068 A SE8303068 A SE 8303068A SE 8303068 A SE8303068 A SE 8303068A SE 459816 B SE459816 B SE 459816B
Authority
SE
Sweden
Prior art keywords
gene
dna
dna fragment
polypeptide
transformation products
Prior art date
Application number
SE8303068A
Other languages
English (en)
Swedish (sv)
Other versions
SE8303068L (sv
SE8303068D0 (sv
Inventor
A Nakata
H Shinagawa
Original Assignee
Univ Osaka Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Osaka Res Found filed Critical Univ Osaka Res Found
Publication of SE8303068D0 publication Critical patent/SE8303068D0/xx
Publication of SE8303068L publication Critical patent/SE8303068L/xx
Publication of SE459816B publication Critical patent/SE459816B/sv

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/61Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales
    • Y10S435/848Escherichia
    • Y10S435/849Escherichia coli

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
SE8303068A 1982-06-04 1983-05-31 Vektor med stark genexpression SE459816B (sv)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP57096775A JPS592689A (ja) 1982-06-04 1982-06-04 強力な遺伝子発現能を有する新規レプリコンの作成法

Publications (3)

Publication Number Publication Date
SE8303068D0 SE8303068D0 (sv) 1983-05-31
SE8303068L SE8303068L (sv) 1983-12-05
SE459816B true SE459816B (sv) 1989-08-07

Family

ID=14174007

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8303068A SE459816B (sv) 1982-06-04 1983-05-31 Vektor med stark genexpression

Country Status (9)

Country Link
US (1) US4703005A (ja)
JP (1) JPS592689A (ja)
BE (1) BE896957A (ja)
CA (1) CA1202258A (ja)
DE (1) DE3320339A1 (ja)
FR (1) FR2528070B1 (ja)
GB (1) GB2124232B (ja)
NL (1) NL189208C (ja)
SE (1) SE459816B (ja)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0699580B2 (ja) * 1986-05-28 1994-12-07 花王株式会社 多孔性フイルム
FR2599380A1 (fr) * 1986-05-29 1987-12-04 Centre Nat Rech Scient Vecteur d'exportation de proteines chez escherichiacoli, bacteries transformees et procede pour la preparation de proteines
JPH0699581B2 (ja) * 1986-06-09 1994-12-07 花王株式会社 多孔性フイルム
JPH0768394B2 (ja) * 1987-12-01 1995-07-26 花王株式会社 多孔性フィルム及びその製造方法
US5139954A (en) * 1988-03-29 1992-08-18 E. I. Du Pont De Nemours And Company DNA promoter fragments from wheat
NZ228320A (en) 1988-03-29 1991-06-25 Du Pont Nucleic acid promoter fragments of the promoter region homologous to the em gene of wheat, dna constructs therefrom and plants thereof
HU218717B (hu) * 1989-03-17 2000-11-28 E. I. Du Pont De Nemours And Co. Nukleinsav-termelést fokozó növényi eredetű génfragmentek és eljárás előállításukra
US5304472A (en) * 1992-11-20 1994-04-19 Genentech, Inc. Method of controlling polypeptide production in bacterial cells
US5965402A (en) * 1996-02-20 1999-10-12 Smithkline Beecham Corporation DNA encoding phoH polypeptides
JP4229341B2 (ja) * 1996-03-23 2009-02-25 財団法人阪大微生物病研究会 破傷風毒素の機能的フラグメント抗原及び破傷風ワクチン
CN107532163A (zh) * 2015-04-24 2018-01-02 味之素株式会社 蛋白质的分泌生产方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4237224A (en) * 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
GB2023612B (en) * 1978-06-01 1982-09-15 Hopwood D A Inc of nucleic acid into cellular systems streptomyces plasmids
US4411994A (en) * 1978-06-08 1983-10-25 The President And Fellows Of Harvard College Protein synthesis
FR2441659A1 (fr) * 1978-11-14 1980-06-13 Anvar Nouveaux plasmides hybrides et microorganismes les contenant
US4273875A (en) * 1979-03-05 1981-06-16 The Upjohn Company Plasmid and process of isolating same
AU542264B2 (en) * 1979-06-01 1985-02-14 G.D. Searle & Co. Plasmid vectors
DE2931999A1 (de) * 1979-08-03 1981-02-26 Schering Ag Herstellung und anwendung von neukombinierten plasmiden mit genen fuer alkalische phosphatasen
NO159863C (no) * 1980-01-07 1989-02-15 Univ Rochester Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme.
US4374927A (en) * 1981-02-24 1983-02-22 The Board Of Trustees Of The Leland Stanford Jr. University Extrachromosomal regulation of expression

Also Published As

Publication number Publication date
DE3320339C2 (ja) 1990-08-23
NL189208B (nl) 1992-09-01
FR2528070A1 (fr) 1983-12-09
NL189208C (nl) 1993-02-01
GB2124232B (en) 1985-12-18
GB8315278D0 (en) 1983-07-06
BE896957A (fr) 1983-12-05
DE3320339A1 (de) 1984-01-19
NL8301986A (nl) 1984-01-02
SE8303068L (sv) 1983-12-05
FR2528070B1 (fr) 1987-07-31
US4703005A (en) 1987-10-27
CA1202258A (en) 1986-03-25
JPH0116159B2 (ja) 1989-03-23
JPS592689A (ja) 1984-01-09
SE8303068D0 (sv) 1983-05-31
GB2124232A (en) 1984-02-15

Similar Documents

Publication Publication Date Title
Dworkin et al. Cell interactions in myxobacterial growth and development
EP0449770B1 (en) Bacterial vectors
JPH0665305B2 (ja) 組換えdna含有宿主細胞の安定化法
Dougan et al. 12 The Minicell System as a Method for Studying Expression from Plasmid DNA
SE459816B (sv) Vektor med stark genexpression
CA1150169A (en) Broad host range small plasmid rings as cloning vehicles
CN116286931B (zh) 用于富养罗尔斯通氏菌快速基因编辑的双质粒系统及应用
CA1314831C (en) Extraction of human interleukin-4 from bacteria
US7405059B2 (en) Modified Shine-Dalgarno sequences and methods of uses thereof
US20040014221A1 (en) Plasmid originated from bifidobacterium, recombinant expression vector using the plasmid and transformation method
JP2551746B2 (ja) 改良プラスミドベクタ−およびその利用
US7030233B2 (en) Ketogulonigenium endogeneous plasmids
US20040175705A1 (en) Systems for expression of heterologous proteins in m capsulatus
US4418194A (en) DNA Fragments for forming plasmids
JP2003235565A (ja) 乳酸菌用シャトルベクター
US7033824B2 (en) Ketogulonigenium shuttle vectors
JP2007228934A (ja) ロドコッカス属に属する細菌の形質転換方法
US20020064871A1 (en) Endogenous ketogulonigenium plasmid
Oudega et al. The use of minicells and maxicells to detect the expression of cloned genes
JP2590422B2 (ja) ラン藻を用いた大腸菌の遺伝子産物の製造方法
JP3876310B2 (ja) リゾチーム感受性新規微生物
CN101743312A (zh) 克隆核酸序列的装置和方法
RU2229517C1 (ru) РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК PZIFN 2α, КОДИРУЮЩАЯ СИНТЕЗ АЛЬФА-2B-ИНТЕРФЕРОНА ЧЕЛОВЕКА, И ШТАММ ESCHERICHIA COLI - ПРОДУЦЕНТ АЛЬФА-2B-ИНТЕРФЕРОНА ЧЕЛОВЕКА
JP3520322B2 (ja) 低温細菌由来の新規なプラスミドpPS1M2及びその誘導体
RU2188233C2 (ru) Штамм бактерий bacillus subtilis pbcole2-продуцент гибридного колицина e2, используемый для получения ветеринарного препарата

Legal Events

Date Code Title Description
NAL Patent in force

Ref document number: 8303068-4

Format of ref document f/p: F

NUG Patent has lapsed