SE449003B - Sett att in vitro avskilja spermier med egen rorelseformaga medelst penetrationsmedium i vilket ingar hyaluronsyrasalt - Google Patents
Sett att in vitro avskilja spermier med egen rorelseformaga medelst penetrationsmedium i vilket ingar hyaluronsyrasaltInfo
- Publication number
- SE449003B SE449003B SE8504872A SE8504872A SE449003B SE 449003 B SE449003 B SE 449003B SE 8504872 A SE8504872 A SE 8504872A SE 8504872 A SE8504872 A SE 8504872A SE 449003 B SE449003 B SE 449003B
- Authority
- SE
- Sweden
- Prior art keywords
- sperm
- medium
- penetration
- layer
- hyaluronic acid
- Prior art date
Links
- 230000035515 penetration Effects 0.000 title claims abstract description 53
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical class CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 title claims abstract description 13
- 238000000338 in vitro Methods 0.000 title claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 22
- 230000004720 fertilization Effects 0.000 claims abstract description 13
- 229920002674 hyaluronan Polymers 0.000 claims abstract description 12
- 229960003160 hyaluronic acid Drugs 0.000 claims abstract description 12
- 150000003839 salts Chemical class 0.000 claims description 15
- 230000004899 motility Effects 0.000 claims description 11
- 101100366942 Mus musculus Ston1 gene Proteins 0.000 abstract 1
- 239000000126 substance Substances 0.000 description 12
- WCDDVEOXEIYWFB-VXORFPGASA-N (2s,3s,4r,5r,6r)-3-[(2s,3r,5s,6r)-3-acetamido-5-hydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-4,5,6-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@@H]1C[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O)[C@H](O)[C@H]1O WCDDVEOXEIYWFB-VXORFPGASA-N 0.000 description 8
- 229940014041 hyaluronate Drugs 0.000 description 8
- 239000000243 solution Substances 0.000 description 7
- 150000002500 ions Chemical class 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 102000009027 Albumins Human genes 0.000 description 5
- 108010088751 Albumins Proteins 0.000 description 5
- 239000000654 additive Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 229920002385 Sodium hyaluronate Polymers 0.000 description 4
- 229940010747 sodium hyaluronate Drugs 0.000 description 4
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 4
- 239000000872 buffer Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 230000002950 deficient Effects 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 230000035558 fertility Effects 0.000 description 3
- 238000013508 migration Methods 0.000 description 3
- 230000005012 migration Effects 0.000 description 3
- 210000002381 plasma Anatomy 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 159000000000 sodium salts Chemical class 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 229910001414 potassium ion Inorganic materials 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 229910001415 sodium ion Inorganic materials 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 101100378191 Caenorhabditis elegans aco-2 gene Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000282461 Canis lupus Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000008100 Human Serum Albumin Human genes 0.000 description 1
- 108091006905 Human Serum Albumin Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- OSWRVYBYIGOAEZ-UHFFFAOYSA-N acetic acid;2-hydroxypropanoic acid Chemical compound CC(O)=O.CC(O)C(O)=O OSWRVYBYIGOAEZ-UHFFFAOYSA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 150000003841 chloride salts Chemical class 0.000 description 1
- 210000001520 comb Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000027758 ovulation cycle Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000019100 sperm motility Effects 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Reproductive Health (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Virology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Surgical Instruments (AREA)
- Media Introduction/Drainage Providing Device (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8504872A SE449003B (sv) | 1985-10-18 | 1985-10-18 | Sett att in vitro avskilja spermier med egen rorelseformaga medelst penetrationsmedium i vilket ingar hyaluronsyrasalt |
| JP61505485A JPS63501056A (ja) | 1985-10-18 | 1986-10-17 | 運動性精子の選択方法および手段 |
| DE8686906029T DE3684462D1 (de) | 1985-10-18 | 1986-10-17 | Trennverfahren fuer motile saatzellen in vitro, befruchtungsverfahren und durchdringungsmedium fuer saatzellen. |
| PCT/SE1986/000484 WO1987002382A1 (en) | 1985-10-18 | 1986-10-17 | Process for separating motile sperm cells in vitro, fertilization method and penetration medium for sperm cells |
| EP86906029A EP0243397B1 (de) | 1985-10-18 | 1986-10-17 | Trennverfahren für motile saatzellen in vitro, befruchtungsverfahren und durchdringungsmedium für saatzellen |
| US07/060,360 US4804537A (en) | 1985-10-18 | 1986-10-17 | Sperm selection process using a salt of hyaluronic acid |
| AT86906029T ATE73843T1 (de) | 1985-10-18 | 1986-10-17 | Trennverfahren fuer motile saatzellen in vitro, befruchtungsverfahren und durchdringungsmedium fuer saatzellen. |
| AU64751/86A AU589702B2 (en) | 1985-10-18 | 1986-10-17 | Process for separating motile sperm cells in vitro, fertilization method and penetration medium for sperm cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8504872A SE449003B (sv) | 1985-10-18 | 1985-10-18 | Sett att in vitro avskilja spermier med egen rorelseformaga medelst penetrationsmedium i vilket ingar hyaluronsyrasalt |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SE8504872D0 SE8504872D0 (sv) | 1985-10-18 |
| SE449003B true SE449003B (sv) | 1987-03-30 |
Family
ID=20361798
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE8504872A SE449003B (sv) | 1985-10-18 | 1985-10-18 | Sett att in vitro avskilja spermier med egen rorelseformaga medelst penetrationsmedium i vilket ingar hyaluronsyrasalt |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US4804537A (de) |
| EP (1) | EP0243397B1 (de) |
| JP (1) | JPS63501056A (de) |
| AT (1) | ATE73843T1 (de) |
| AU (1) | AU589702B2 (de) |
| DE (1) | DE3684462D1 (de) |
| SE (1) | SE449003B (de) |
| WO (1) | WO1987002382A1 (de) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE8901127D0 (sv) * | 1989-03-31 | 1989-03-31 | Aa H L Pousette | Reagenssats och dess anvaending foer att bilda (spap-anti-spap) -immunkomplex samt diagnostiskt foerfarand vid vilket spap detekteras |
| US5102783A (en) * | 1990-01-12 | 1992-04-07 | Vetrepharm, Inc. | Composition and method for culturing and freezing cells and tissues |
| EP0446509A1 (de) * | 1990-03-15 | 1991-09-18 | Panayiotis M. Zavos | Sperma-Säulenfilter |
| CA2061703C (en) * | 1992-02-20 | 2002-07-02 | Rudolf E. Falk | Formulations containing hyaluronic acid |
| US5910489A (en) * | 1990-09-18 | 1999-06-08 | Hyal Pharmaceutical Corporation | Topical composition containing hyaluronic acid and NSAIDS |
| US5990096A (en) * | 1990-09-18 | 1999-11-23 | Hyal Pharmaceutical Corporation | Formulations containing hyaluronic acid |
| US5824658A (en) * | 1990-09-18 | 1998-10-20 | Hyal Pharmaceutical Corporation | Topical composition containing hyaluronic acid and NSAIDS |
| US5977088A (en) * | 1991-07-03 | 1999-11-02 | Hyal Pharmaceutical Corporation | Formulations containing hyaluronic acid |
| US5792753A (en) * | 1991-07-03 | 1998-08-11 | Hyal Pharmaceutical Corporation | Compositions comprising hyaluronic acid and prostaglandin-synthesis-inhibiting drugs |
| US6103704A (en) * | 1991-07-03 | 2000-08-15 | Hyal Pharmaceutical Corporation | Therapeutic methods using hyaluronic acid |
| US6218373B1 (en) | 1992-02-20 | 2001-04-17 | Hyal Pharmaceutical Corporation | Formulations containing hyaluronic acid |
| US5767106A (en) * | 1992-02-21 | 1998-06-16 | Hyal Pharmaceutical Corporation | Treatment of disease and conditions associated with macrophage infiltration |
| ES2044778B1 (es) * | 1992-04-02 | 1994-09-01 | Esteve Carmen Blanco | Metodo analitico para la determinacion de la capacidad fecundante de una muestra de semen humano. |
| US5744366A (en) * | 1992-05-01 | 1998-04-28 | Trustees Of The University Of Pennsylvania | Mesoscale devices and methods for analysis of motile cells |
| US5801058A (en) * | 1994-06-20 | 1998-09-01 | Blanco-Esteve; Carmen | Analytic method to determine the fertilizing capacity of a human semen sample |
| US5935800A (en) * | 1994-10-31 | 1999-08-10 | Beth Israel Deaconess Medical Center | Assays and kits for determining male fertility |
| US5895749A (en) * | 1994-10-31 | 1999-04-20 | Beth Israel Deaconess Medical Center | Male fertility and contraception home test kits |
| WO1996013225A2 (en) * | 1994-10-31 | 1996-05-09 | Beth Israel Hospital Association | Assays, devices and kits for determining male fertility |
| AU723170B2 (en) * | 1995-10-19 | 2000-08-17 | Bio-Origyn Llc | Methods and compositions to improve germ cell and embryo survival and function |
| US6129214A (en) * | 1997-12-19 | 2000-10-10 | Bar-Ami; Shalom | Sperm strainer system |
| US5897988A (en) * | 1998-01-21 | 1999-04-27 | Yale University | Process and system for selection of mature sperm by surface membrane determinants for assisted reproduction |
| GB9817795D0 (en) * | 1998-08-14 | 1998-10-14 | Genosis Inc | A method and device for separation and detection of spermatozoa in a sample |
| WO2000032140A1 (en) * | 1998-11-30 | 2000-06-08 | Ivf Sciences Colorado, Inc. | System and sequential culture media for in vitro fertilization |
| US6762053B2 (en) * | 2000-06-09 | 2004-07-13 | Vitrolife, Inc. | Mammalian gamete and embryo culture media and culture media supplements |
| ES2259566B1 (es) * | 2005-03-29 | 2007-08-01 | Instituto Nacional De Investigacion Y Tecnologia Agraria Y Alimentaria-Inia | Suplementacion para los medios de manipulacion embrionaria y/o celular. |
| CN101487841B (zh) * | 2009-02-13 | 2014-06-04 | 深圳市人民医院 | 一种包被载体及其在检测精子成熟度和无创分离成熟精子方法中的应用 |
| ES2387903B1 (es) * | 2010-07-16 | 2013-08-13 | María Albiñana Blanco | Procedimiento y kit para verificar, en una muestra de semen humano o animal, la existencia de espermatozoides fecundantes. |
| CN102323398B (zh) * | 2011-05-19 | 2014-03-12 | 国家人口计生委科学技术研究所 | 一种用于精子检测的溶液 |
| RU2662080C1 (ru) * | 2017-09-18 | 2018-07-23 | Общество с ограниченной ответственностью "Генетико-селекционный исследовательский центр "Генология-МГУ" | Способ отбора генетически полноценных сперматозоидов сельскохозяйственных животных |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4327177A (en) * | 1969-04-10 | 1982-04-27 | Wallace Shrimpton | Method and means for controlling the sex of mammalian offspring and product therefor |
| US4009260A (en) * | 1973-04-19 | 1977-02-22 | Schering Aktiengesellschaft | Fractionation of sperm |
| US4007087A (en) * | 1975-10-17 | 1977-02-08 | Gametrics Limited | Sperm fractionation and storage |
| US4339434A (en) * | 1981-08-17 | 1982-07-13 | Gametrics Limited | Method of increasing the incidence of female offspring |
-
1985
- 1985-10-18 SE SE8504872A patent/SE449003B/sv not_active IP Right Cessation
-
1986
- 1986-10-17 WO PCT/SE1986/000484 patent/WO1987002382A1/en not_active Ceased
- 1986-10-17 US US07/060,360 patent/US4804537A/en not_active Expired - Lifetime
- 1986-10-17 AU AU64751/86A patent/AU589702B2/en not_active Expired
- 1986-10-17 AT AT86906029T patent/ATE73843T1/de not_active IP Right Cessation
- 1986-10-17 JP JP61505485A patent/JPS63501056A/ja active Granted
- 1986-10-17 DE DE8686906029T patent/DE3684462D1/de not_active Expired - Lifetime
- 1986-10-17 EP EP86906029A patent/EP0243397B1/de not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| AU589702B2 (en) | 1989-10-19 |
| JPH0317492B2 (de) | 1991-03-08 |
| EP0243397A1 (de) | 1987-11-04 |
| EP0243397B1 (de) | 1992-03-18 |
| AU6475186A (en) | 1987-05-05 |
| JPS63501056A (ja) | 1988-04-21 |
| WO1987002382A1 (en) | 1987-04-23 |
| US4804537A (en) | 1989-02-14 |
| ATE73843T1 (de) | 1992-04-15 |
| SE8504872D0 (sv) | 1985-10-18 |
| DE3684462D1 (de) | 1992-04-23 |
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