SE448259B - Forfarande for bestemning av ligander i testprover och analytiskt reagens dertill - Google Patents
Forfarande for bestemning av ligander i testprover och analytiskt reagens dertillInfo
- Publication number
- SE448259B SE448259B SE8000843A SE8000843A SE448259B SE 448259 B SE448259 B SE 448259B SE 8000843 A SE8000843 A SE 8000843A SE 8000843 A SE8000843 A SE 8000843A SE 448259 B SE448259 B SE 448259B
- Authority
- SE
- Sweden
- Prior art keywords
- enzyme
- solution
- irreversible
- compound
- added
- Prior art date
Links
- 239000003446 ligand Substances 0.000 title claims description 37
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 28
- 238000000034 method Methods 0.000 title claims description 23
- 238000012360 testing method Methods 0.000 title claims description 16
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- 238000004458 analytical method Methods 0.000 claims description 4
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- GFFIJCYHQYHUHB-UHFFFAOYSA-N 2-acetylsulfanylethyl(trimethyl)azanium Chemical group CC(=O)SCC[N+](C)(C)C GFFIJCYHQYHUHB-UHFFFAOYSA-N 0.000 claims description 2
- 102000014914 Carrier Proteins Human genes 0.000 claims 1
- 230000002779 inactivation Effects 0.000 claims 1
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- 125000001475 halogen functional group Chemical group 0.000 description 4
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- WVWHRXVVAYXKDE-UHFFFAOYSA-N piperine Natural products O=C(C=CC=Cc1ccc2OCOc2c1)C3CCCCN3 WVWHRXVVAYXKDE-UHFFFAOYSA-N 0.000 description 1
- 235000019100 piperine Nutrition 0.000 description 1
- 229960005235 piperonyl butoxide Drugs 0.000 description 1
- 229920003217 poly(methylsilsesquioxane) Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 150000004804 polysaccharides Polymers 0.000 description 1
- 238000011176 pooling Methods 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 229910000027 potassium carbonate Inorganic materials 0.000 description 1
- 239000008057 potassium phosphate buffer Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000186 progesterone Substances 0.000 description 1
- 229960003387 progesterone Drugs 0.000 description 1
- NSETWVJZUWGCKE-UHFFFAOYSA-N propylphosphonic acid Chemical compound CCCP(O)(O)=O NSETWVJZUWGCKE-UHFFFAOYSA-N 0.000 description 1
- 150000003180 prostaglandins Chemical class 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 229960001404 quinidine Drugs 0.000 description 1
- MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 description 1
- 125000005493 quinolyl group Chemical class 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000013391 scatchard analysis Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229940076279 serotonin Drugs 0.000 description 1
- 238000010898 silica gel chromatography Methods 0.000 description 1
- 239000012312 sodium hydride Substances 0.000 description 1
- 229910000104 sodium hydride Inorganic materials 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229960002317 succinimide Drugs 0.000 description 1
- 229940124530 sulfonamide Drugs 0.000 description 1
- 150000003456 sulfonamides Chemical class 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-O sulfonium Chemical compound [SH3+] RWSOTUBLDIXVET-UHFFFAOYSA-O 0.000 description 1
- 229910052717 sulfur Chemical group 0.000 description 1
- 239000011593 sulfur Chemical group 0.000 description 1
- YBBRCQOCSYXUOC-UHFFFAOYSA-N sulfuryl dichloride Chemical compound ClS(Cl)(=O)=O YBBRCQOCSYXUOC-UHFFFAOYSA-N 0.000 description 1
- 229910021653 sulphate ion Inorganic materials 0.000 description 1
- 229960000278 theophylline Drugs 0.000 description 1
- 150000007970 thio esters Chemical class 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 229960004072 thrombin Drugs 0.000 description 1
- 229940034208 thyroxine Drugs 0.000 description 1
- XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- NLVFBUXFDBBNBW-PBSUHMDJSA-N tobramycin Chemical compound N[C@@H]1C[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N NLVFBUXFDBBNBW-PBSUHMDJSA-N 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 150000005691 triesters Chemical class 0.000 description 1
- ZMANZCXQSJIPKH-UHFFFAOYSA-O triethylammonium ion Chemical compound CC[NH+](CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-O 0.000 description 1
- ILWRPSCZWQJDMK-UHFFFAOYSA-N triethylazanium;chloride Chemical compound Cl.CCN(CC)CC ILWRPSCZWQJDMK-UHFFFAOYSA-N 0.000 description 1
- 229940035722 triiodothyronine Drugs 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 201000008297 typhoid fever Diseases 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000003039 volatile agent Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/28—Phosphorus compounds with one or more P—C bonds
- C07F9/38—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)]
- C07F9/40—Esters thereof
- C07F9/4071—Esters thereof the ester moiety containing a substituent or a structure which is considered as characteristic
- C07F9/4075—Esters with hydroxyalkyl compounds
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/825—Pretreatment for removal of interfering factors from sample
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Organic Chemistry (AREA)
- Steroid Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/009,007 US4273866A (en) | 1979-02-05 | 1979-02-05 | Ligand analog-irreversible enzyme inhibitor conjugates and methods for use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SE8000843L SE8000843L (sv) | 1980-08-06 |
| SE448259B true SE448259B (sv) | 1987-02-02 |
Family
ID=21735028
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE8000843A SE448259B (sv) | 1979-02-05 | 1980-02-01 | Forfarande for bestemning av ligander i testprover och analytiskt reagens dertill |
Country Status (15)
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56501583A (US06724976-20040420-M00002.png) * | 1979-11-19 | 1981-10-29 | ||
| DE3006709A1 (de) * | 1980-02-22 | 1981-08-27 | Hans A. Dipl.-Chem. Dr. 8000 München Thoma | Homogenes verfahren zur kompetitiven bestimmung von liganden |
| US4341865A (en) * | 1980-07-21 | 1982-07-27 | Abbott Laboratories | Determination of thyroxine binding globulin |
| US4849353A (en) * | 1980-09-30 | 1989-07-18 | Cornell Research Foundation, Inc. | Immunocapture of enzyme inhibitor, enzyme complexes and uses thereof |
| US4323647A (en) * | 1980-10-15 | 1982-04-06 | University Of Miami | Steric hindrance enzyme immunoassay |
| DE3100062A1 (de) * | 1981-01-02 | 1982-08-05 | Hans A. Dipl.-Chem. Dr. 8000 München Thoma | Homogenes verfahren zur bestimmung von liganden |
| DE3100061A1 (de) * | 1981-01-02 | 1982-08-05 | Hans A. Dipl.-Chem. Dr. 8000 München Thoma | Verfahren zur bestimmung von liganden mittels kompetitionsreaktion |
| EP0079489A1 (en) * | 1981-11-04 | 1983-05-25 | Miles Laboratories, Inc. | Methotrexate-labeled iodothyronine conjugates and method, reagent means and test device for the determination of iodothyronine in or iodothyronine uptake of a liquid sample |
| US4451652A (en) * | 1982-05-24 | 1984-05-29 | Abbott Laboratories | Substituted theophylline salts |
| US4472301A (en) * | 1982-05-27 | 1984-09-18 | Miles Laboratories, Inc. | Propranolol immunogen and antibodies |
| GB2135773B (en) * | 1983-01-31 | 1985-12-04 | Boots Celltech Diagnostics | Enzyme inhibitor labelled immunoassay |
| US4650751A (en) * | 1983-04-29 | 1987-03-17 | Technicon Instruments Corporation | Protected binding assay avoiding non-specific protein interference |
| US4550075A (en) * | 1983-06-22 | 1985-10-29 | Kallestad Laboratories, Inc. | Method for ligand determination utilizing an immunoassay monitorable by biotin-containing enzymes, and compositions therefor |
| JPS607362A (ja) * | 1983-06-27 | 1985-01-16 | Fujirebio Inc | 酵素を用いた抗原決定基具有物質の測定法 |
| US4629694A (en) * | 1983-07-12 | 1986-12-16 | Cornell Research Foundation, Inc. | Detecting and distinguishing between plasminogen activators |
| US4543412A (en) * | 1983-11-04 | 1985-09-24 | Abbott Laboratories | Phenobarbital enzyme inhibitors |
| IL73293A (en) * | 1983-11-04 | 1988-02-29 | Abbott Lab | Methyl 2-(alkanamidoalkylsulfonic)ethylthiophosphonate derivatives |
| US4559173A (en) * | 1983-11-04 | 1985-12-17 | Abbott Laboratories | N-substituted dibenz[b,f]azepine salts |
| DE4007048A1 (de) * | 1990-03-07 | 1991-09-12 | Hoechst Ag | N-acyl-aminoalkyl-2-hydroxyethylsulfide und ein verfahren zu ihrer herstellung |
| US5338663A (en) * | 1990-08-24 | 1994-08-16 | President And Fellows Of Harvard College | Method of identifying inhibitors of β-protein esterase activity |
| US5648462A (en) * | 1990-10-09 | 1997-07-15 | Setsuko Funakoshi | Peptide purification method using novel linker and solid-phase ligand |
| JP2552049B2 (ja) * | 1990-10-09 | 1996-11-06 | 舩越 節子 | 合成ペプチド精製方法およびこれに用いるリンカー、リンカー結合用固相担体 |
| JPH0737982B2 (ja) * | 1992-12-14 | 1995-04-26 | 元成 狩野 | 抗胆汁酸抗体およびこれを用いた糞便中の胆汁酸検査方法 |
| US5922703A (en) * | 1993-09-24 | 1999-07-13 | The Procter & Gamble Company | Urethane-containing aminosteroid compounds |
| EP0746557A4 (en) * | 1994-02-18 | 1997-05-02 | Cell Therapeutics Inc | INTRACELLULAR SIGNALING MEDIATORS |
| US6811998B2 (en) * | 1999-06-25 | 2004-11-02 | Roche Diagnostics Operations, Inc. | Conjugates of uncompetitive inhibitors of inosine monophosphate dehydrogenase |
| ES2298146T3 (es) * | 1999-06-25 | 2008-05-16 | Roche Diagnostics Operations, Inc. | Inmunoensayo de inhibicion de enzimas. |
| US6524808B1 (en) * | 1999-06-25 | 2003-02-25 | Roche Diagnostics Corporation | Enzyme inhibition immunoassay |
| US8642273B2 (en) * | 2002-04-16 | 2014-02-04 | The Regents Of The University Of California | Ligand sensing fluorescent acetylcholinesterase for detection of organophosphate activity |
| CN103980338B (zh) | 2010-01-15 | 2017-04-26 | 苏州润新生物科技有限公司 | 蟾蜍灵衍生物、其药物组合物及用途 |
| WO2012027957A1 (en) | 2010-08-28 | 2012-03-08 | Suzhou Neupharma Co., Ltd. | Bufalin derivatives, pharmaceutical compositions and use thereof |
| EP2670763B1 (en) | 2011-02-02 | 2018-08-01 | Suzhou Neupharma Co., Ltd | Certain chemical entities, compositions, and methods |
| WO2013165924A1 (en) | 2012-04-29 | 2013-11-07 | Neupharma, Inc. | Certain chemical entities, compositions, and methods |
| CN112114127A (zh) * | 2020-09-09 | 2020-12-22 | 武汉生之源生物科技股份有限公司 | 一种甘胆酸均相酶免疫测定试剂盒及其制备方法和应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IN142734B (US06724976-20040420-M00002.png) * | 1975-04-28 | 1977-08-20 | Miles Lab | |
| NL7512939A (nl) * | 1975-11-04 | 1977-05-06 | Tno | Werkwijze en inrichtingen voor het aantonen en/of bepalen van alkylerende verbindingen, zoals mosterdgassen. |
| US4134792A (en) | 1976-12-06 | 1979-01-16 | Miles Laboratories, Inc. | Specific binding assay with an enzyme modulator as a labeling substance |
| GB1595101A (en) * | 1977-03-15 | 1981-08-05 | Hoffmann La Roche | Enzyme modifier immunoassay |
| IT1105734B (it) * | 1977-07-14 | 1985-11-04 | Syva Co | Prova di legame di competizione di antienzima omogeneo |
-
1979
- 1979-02-05 US US06/009,007 patent/US4273866A/en not_active Expired - Lifetime
-
1980
- 1980-01-15 CA CA000343676A patent/CA1137077A/en not_active Expired
- 1980-01-21 AU AU54763/80A patent/AU528592B2/en not_active Ceased
- 1980-01-22 ZA ZA00800371A patent/ZA80371B/xx unknown
- 1980-01-28 GB GB8002743A patent/GB2043245B/en not_active Expired
- 1980-02-01 JP JP1011780A patent/JPS55104896A/ja active Pending
- 1980-02-01 AT AT0056080A patent/AT365781B/de not_active IP Right Cessation
- 1980-02-01 SE SE8000843A patent/SE448259B/sv not_active IP Right Cessation
- 1980-02-04 CH CH87780A patent/CH641569A5/fr not_active IP Right Cessation
- 1980-02-04 DE DE3003959A patent/DE3003959C2/de not_active Expired
- 1980-02-04 ES ES488263A patent/ES488263A0/es active Granted
- 1980-02-04 IT IT19675/80A patent/IT1130254B/it active
- 1980-02-04 FR FR8002385A patent/FR2447966A1/fr active Granted
- 1980-02-04 NL NL8000698A patent/NL8000698A/nl not_active Application Discontinuation
- 1980-02-05 BE BE0/199272A patent/BE881557A/fr not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| ES8101647A1 (es) | 1980-12-16 |
| CH641569A5 (fr) | 1984-02-29 |
| AT365781B (de) | 1982-02-10 |
| FR2447966A1 (fr) | 1980-08-29 |
| ATA56080A (de) | 1981-06-15 |
| IT1130254B (it) | 1986-06-11 |
| CA1137077A (en) | 1982-12-07 |
| IT8019675A0 (it) | 1980-02-04 |
| DE3003959C2 (de) | 1982-12-23 |
| BE881557A (fr) | 1980-08-05 |
| AU5476380A (en) | 1980-08-14 |
| GB2043245A (en) | 1980-10-01 |
| SE8000843L (sv) | 1980-08-06 |
| DE3003959A1 (de) | 1980-08-14 |
| JPS55104896A (en) | 1980-08-11 |
| FR2447966B1 (US06724976-20040420-M00002.png) | 1984-10-19 |
| NL8000698A (nl) | 1980-08-07 |
| GB2043245B (en) | 1983-05-25 |
| US4273866A (en) | 1981-06-16 |
| ZA80371B (en) | 1981-03-25 |
| AU528592B2 (en) | 1983-05-05 |
| ES488263A0 (es) | 1980-12-16 |
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