RU96105702A - ПОСЛЕДОВАТЕЛЬНОСТЬ ДНК, ТРАНСФОРМИРОВАННАЯ КЛЕТКА, СПОСОБ ПОЛУЧЕНИЯ РЕКОМБИНАНТНОЙ ПЕПТИДИЛГЛИЦИНα-АМИДИРУЮЩЕЙ МОНООКСИГЕНАЗЫ И СПОСОБ ПОЛУЧЕНИЯ АМИДИРОВАННЫХ ПЕПТИДОВ - Google Patents
ПОСЛЕДОВАТЕЛЬНОСТЬ ДНК, ТРАНСФОРМИРОВАННАЯ КЛЕТКА, СПОСОБ ПОЛУЧЕНИЯ РЕКОМБИНАНТНОЙ ПЕПТИДИЛГЛИЦИНα-АМИДИРУЮЩЕЙ МОНООКСИГЕНАЗЫ И СПОСОБ ПОЛУЧЕНИЯ АМИДИРОВАННЫХ ПЕПТИДОВInfo
- Publication number
- RU96105702A RU96105702A RU96105702/13A RU96105702A RU96105702A RU 96105702 A RU96105702 A RU 96105702A RU 96105702/13 A RU96105702/13 A RU 96105702/13A RU 96105702 A RU96105702 A RU 96105702A RU 96105702 A RU96105702 A RU 96105702A
- Authority
- RU
- Russia
- Prior art keywords
- producing
- peptidylglycine
- dna sequence
- monooxygenase
- amiding
- Prior art date
Links
- 229920001850 Nucleic acid sequence Polymers 0.000 title claims 3
- 108010074633 Mixed Function Oxygenases Proteins 0.000 title claims 2
- 102000008109 Mixed Function Oxygenases Human genes 0.000 title claims 2
- 238000004519 manufacturing process Methods 0.000 title 2
- 229920001192 peptidylglycine Polymers 0.000 title 1
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 title 1
- 102100001279 PAM Human genes 0.000 claims 5
- 108010007262 peptidylglycine monooxygenase Proteins 0.000 claims 5
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Natural products NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims 1
- 239000004471 Glycine Substances 0.000 claims 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 claims 1
- 239000002751 oligonucleotide probe Substances 0.000 claims 1
- OZAIFHULBGXAKX-UHFFFAOYSA-N precursor Substances N#CC(C)(C)N=NC(C)(C)C#N OZAIFHULBGXAKX-UHFFFAOYSA-N 0.000 claims 1
Claims (4)
1. Изолированная последовательность ДНК, кодирующая пептидилглицинальфа-амидирующую монооксигеназу, содержащая в своем составе по крайней мере одну субчасть, выбранную из группы I, приведенной на c.
и гибридизующаяся с по крайней мере двумя олигонуклеотидными зондами, выбранными из группы II, приведенной на c.
2. Хозяйская клетка, трансформированная или трансфецированная экспрессирующим вектором, содержащим последовательность ДНК по п. 1.
3. Способ экспрессии рекомбинантной пептидилглицин-альфа-амидирующей монооксигеназы (PAM), отличающийся тем, что осуществляют культивирование хозяйских клеток по п. 2 в течение времени, достаточного для экспрессии PAM, и последующее извлечение PAM.
4. Способ получения амидированного пептида, отличающийся тем, что осуществляют контактирование предшественника пептида, имеющего C-концевой глицин, с пептидилглицин-альфа-амидирующей монооксигеназой, полученной способом по п. 3.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US86161 | 1987-08-14 | ||
| US07/086,161 US6319685B1 (en) | 1984-09-27 | 1987-08-14 | Alpha-amidating enzyme compositions and processes for their production and use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU96105702A true RU96105702A (ru) | 1998-10-27 |
| RU2252961C2 RU2252961C2 (ru) | 2005-05-27 |
Family
ID=22196693
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU884356445A RU2080385C1 (ru) | 1987-08-14 | 1988-08-12 | α -АМИДИРУЮЩАЯ МОНООКСИГЕНАЗА (ВАРИАНТЫ) И СПОСОБ ПОЛУЧЕНИЯ a -АМИДИРОВАННОЙ ФОРМЫ РЕГУЛЯТОРНОГО ПОЛИПЕПТИДА |
| RU96105702/13A RU2252961C2 (ru) | 1987-08-14 | 1988-08-12 | Способ получения амидированного пептида |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU884356445A RU2080385C1 (ru) | 1987-08-14 | 1988-08-12 | α -АМИДИРУЮЩАЯ МОНООКСИГЕНАЗА (ВАРИАНТЫ) И СПОСОБ ПОЛУЧЕНИЯ a -АМИДИРОВАННОЙ ФОРМЫ РЕГУЛЯТОРНОГО ПОЛИПЕПТИДА |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US6319685B1 (ru) |
| EP (2) | EP0529742B1 (ru) |
| JP (1) | JP2979172B2 (ru) |
| KR (1) | KR0136611B1 (ru) |
| AT (1) | ATE198355T1 (ru) |
| CA (1) | CA1341454C (ru) |
| DE (1) | DE3856446T2 (ru) |
| DK (1) | DK175538B1 (ru) |
| FI (1) | FI883722A (ru) |
| HU (3) | HU213227B (ru) |
| LT (1) | LT4029B (ru) |
| NO (1) | NO883592L (ru) |
| RU (2) | RU2080385C1 (ru) |
Families Citing this family (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4708934A (en) * | 1984-09-27 | 1987-11-24 | Unigene Laboratories, Inc. | α-amidation enzyme |
| US6319685B1 (en) | 1984-09-27 | 2001-11-20 | Unigene Laboratories, Inc. | Alpha-amidating enzyme compositions and processes for their production and use |
| US5789234A (en) * | 1987-08-14 | 1998-08-04 | Unigene Laboratories, Inc. | Expression systems for amidating enzyme |
| US5871995A (en) * | 1989-08-15 | 1999-02-16 | Shiseido Company, Ltd. | Purified enzymes participating in C-terminal amidation |
| DK0465404T3 (da) * | 1990-06-01 | 1995-12-18 | Ciba Geigy Ag | Peptidylhydroxylglycin-N-C-lyase og DNA, som koder derfor |
| US5580751A (en) * | 1990-09-14 | 1996-12-03 | Carlsberg A/S | Process for the preparation of C-terminally amidated peptides |
| DK67691D0 (da) * | 1991-03-01 | 1991-04-15 | Carlbiotech Ltd As | Enzymatisk fremgangsmaade til c-terminal modificering af peptider og mellemprodukter til brug ved fremgangsmaaden |
| WO1996017941A2 (en) * | 1994-12-07 | 1996-06-13 | Bionebraska, Inc. | Production of c-terminal amidated peptides from recombinant protein constructs |
| US5912014A (en) * | 1996-03-15 | 1999-06-15 | Unigene Laboratories, Inc. | Oral salmon calcitonin pharmaceutical products |
| ES2375330T3 (es) * | 1997-04-16 | 2012-02-28 | Unigene Laboratories, Inc. | Expresión directa de péptidos en medios de cultivo. |
| WO2004011652A1 (ja) | 2002-07-26 | 2004-02-05 | Ajinomoto Co., Inc. | トリペプチド以上のペプチドの製造方法 |
| US8088734B2 (en) | 2003-01-21 | 2012-01-03 | Unigene Laboratories Inc. | Oral delivery of peptides |
| US7648965B2 (en) * | 2004-05-14 | 2010-01-19 | Unigene Laboratories Inc. | Method for fostering bone formation and preservation |
| US7531518B2 (en) * | 2004-05-14 | 2009-05-12 | Unigene Laboratories Inc. | Method for fostering bone formation and preservation |
| US20060089723A1 (en) * | 2004-10-25 | 2006-04-27 | Murphy Kieran P | Method for bone augmentation |
| US7445911B2 (en) * | 2004-11-24 | 2008-11-04 | Unigene Laboratories Inc. | Enzymatic reactions in the presence of keto acids |
| PA8660701A1 (es) | 2005-02-04 | 2006-09-22 | Pfizer Prod Inc | Agonistas de pyy y sus usos |
| US20060293667A1 (en) * | 2005-05-19 | 2006-12-28 | Agnes Vignery | Bone implant device and methods of using same |
| US8377863B2 (en) | 2007-05-29 | 2013-02-19 | Unigene Laboratories Inc. | Peptide pharmaceutical for oral delivery |
| AU2010206614B2 (en) | 2009-01-22 | 2015-03-26 | Keybioscience Ag | Treatment for obesity |
| RU2012126101A (ru) | 2009-11-23 | 2013-12-27 | АМИЛИН ФАРМАСЬЮТИКАЛЗ, ЭлЭлСи,США | Полипептидный конъюгат |
| TW201138808A (en) | 2010-05-03 | 2011-11-16 | Bristol Myers Squibb Co | Serum albumin binding molecules |
| PT2621519T (pt) | 2010-09-28 | 2017-10-04 | Aegerion Pharmaceuticals Inc | Polipéptido de fusão de leptina-abd com duração de ação melhorada |
| BR112013007442A2 (pt) | 2010-09-28 | 2019-09-24 | Amylin Pharmaceuticals Llc | polipeptídeos construídos tendo duração de ação realçada |
| EP3533457B1 (en) | 2011-06-15 | 2021-08-11 | ResoTher Pharma ApS | Anti-inflammatory pharmaceutical products |
| WO2013009539A1 (en) | 2011-07-08 | 2013-01-17 | Amylin Pharmaceuticals, Inc. | Engineered polypeptides having enhanced duration of action and reduced immunogenicity |
| WO2013009545A1 (en) | 2011-07-08 | 2013-01-17 | Amylin Pharmaceuticals, Inc. | Engineered polypeptides having enhanced duration of action with reduced immunogenicity |
| LT3095484T (lt) | 2011-11-02 | 2018-08-10 | Keybioscience Ag | Kalcitonino mimetikai, skirti ligų ir sutrikimų gydymui |
| US9533022B2 (en) | 2011-11-02 | 2017-01-03 | KeyBioscience A/S | Peptide analogs for treating diseases and disorders |
| KR102019911B1 (ko) | 2011-11-02 | 2019-09-09 | 키바이오사이언스 아게 | 질병 및 장애 치료용 펩타이드 유사체 |
| LT3068796T (lt) | 2013-11-14 | 2018-05-10 | Keybioscience Ag | Kalcitonino mimetikai, skirti ligų ir sutrikimų gydymui |
| CA2932408A1 (en) | 2013-12-02 | 2015-06-11 | Stealth Biotherapeutics Corp | Compositions and methods for treating vitiligo |
| WO2015183995A2 (en) | 2014-05-28 | 2015-12-03 | Stealth Peptides International, Inc. | Therapeutic compositions including frataxin, lactoferrin, and mitochondrial energy generating enzymes, and uses thereof |
| CA2950410A1 (en) | 2014-05-28 | 2015-12-03 | D. Travis Wilson | Therapeutic compositions including therapeutic small molecules and uses thereof |
| CA2952615A1 (en) | 2014-06-17 | 2015-12-23 | Stealth Biotherapeutics Corp | Methods of identifying and monitoring mitochondrial dysfunction using monocyte screening |
| GB201500263D0 (en) | 2015-01-08 | 2015-02-25 | Keybioscience Ag | Calcitonin analogues for treating diseases and disorders |
| WO2016115082A1 (en) | 2015-01-12 | 2016-07-21 | Enteris Biopharma, Inc. | Solid oral dosage forms |
| GB201704429D0 (en) | 2017-03-21 | 2017-05-03 | Keybioscience Ag | Calcitonin mimetics for treating diseases and disorders |
| GB201707955D0 (en) | 2017-05-18 | 2017-07-05 | Keybioscience Ag | Dual amylin and calcitonin receptor agonists for treating diseases and disorders |
| GB201813677D0 (en) | 2018-08-22 | 2018-10-03 | Keybioscience Ag | Calcitonin mimetics for treating diseases and disorders |
| GB201813678D0 (en) | 2018-08-22 | 2018-10-03 | Keybioscience Ag | Acylated calcitonin mimetics |
| GB202001024D0 (en) | 2020-01-24 | 2020-03-11 | Key Bioscience Ag | Oxyntomodulin mimetics |
| WO2022266467A2 (en) | 2021-06-17 | 2022-12-22 | Dana-Farber Cancer Institute, Inc. | Recombinant histone polypeptide and uses thereof |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US655366A (en) | 1900-05-16 | 1900-08-07 | Simeon C Lawlor | Window-cleaner. |
| US4518693A (en) * | 1982-11-01 | 1985-05-21 | Research Corporation | Immobilized biocatalysts |
| AU570600B2 (en) | 1983-06-15 | 1988-03-17 | Celltech Limited | Peptides, pharmaceutical compositions,genes,vectors,host organisms, processes for there production and diagnostic reagents |
| GB2141430B (en) | 1983-06-15 | 1987-01-21 | Kingdon Craig R | Peptides, pharmaceutical compositions, genes, vectors, host organisms, processes for their production and diagnostic reagents |
| US4549986A (en) | 1983-12-23 | 1985-10-29 | The Salk Institute For Biological Studies | Human CGRP |
| US4708934A (en) | 1984-09-27 | 1987-11-24 | Unigene Laboratories, Inc. | α-amidation enzyme |
| US6319685B1 (en) | 1984-09-27 | 2001-11-20 | Unigene Laboratories, Inc. | Alpha-amidating enzyme compositions and processes for their production and use |
| JPS6229997A (ja) | 1985-04-08 | 1987-02-07 | Sankyo Co Ltd | Ｃ末端にプロリンアミドを有するペプチドの製法 |
| GB8523156D0 (en) | 1985-09-19 | 1985-10-23 | Celltech Ltd | Polypeptide production |
| JPS62177184A (ja) | 1986-01-29 | 1987-08-04 | Toyota Motor Corp | 鋳鉄製内燃機関用シリンダヘツドおよびその製造方法 |
| JPH0775541B2 (ja) | 1986-06-07 | 1995-08-16 | 壽之 松尾 | Ｃ末端アミド化酵素及びその製造方法 |
| AU7410987A (en) | 1986-06-12 | 1988-01-07 | Immunex Corp. | Polypeptides devoid of hydrophobic amino acids |
| JP2598050B2 (ja) | 1987-07-17 | 1997-04-09 | サントリー株式会社 | Ｃ−末端α−アミド化酵素 |
| US6255067B1 (en) | 1987-09-15 | 2001-07-03 | The Johns Hopkins University | cDNA encoding peptidyl-glycine alpha-amidating monooxygenase (PAM) |
| JP2845468B2 (ja) | 1989-01-17 | 1999-01-13 | サントリー株式会社 | ヒト甲状腺由来Ｃ―末端α―アミド化酵素 |
-
1987
- 1987-08-14 US US07/086,161 patent/US6319685B1/en not_active Expired - Lifetime
-
1988
- 1988-08-09 HU HU884132A patent/HU213227B/hu unknown
- 1988-08-10 FI FI883722A patent/FI883722A/fi not_active Application Discontinuation
- 1988-08-11 DK DK198804489A patent/DK175538B1/da not_active IP Right Cessation
- 1988-08-12 EP EP92202879A patent/EP0529742B1/en not_active Expired - Lifetime
- 1988-08-12 NO NO88883592A patent/NO883592L/no unknown
- 1988-08-12 CA CA000574685A patent/CA1341454C/en not_active Expired - Lifetime
- 1988-08-12 AT AT92202879T patent/ATE198355T1/de not_active IP Right Cessation
- 1988-08-12 RU SU884356445A patent/RU2080385C1/ru active
- 1988-08-12 RU RU96105702/13A patent/RU2252961C2/ru active
- 1988-08-12 DE DE3856446T patent/DE3856446T2/de not_active Expired - Lifetime
- 1988-08-12 EP EP88307533A patent/EP0308067A3/en not_active Withdrawn
- 1988-08-13 KR KR1019880010344A patent/KR0136611B1/ko not_active IP Right Cessation
- 1988-08-15 JP JP63203037A patent/JP2979172B2/ja not_active Expired - Lifetime
-
1993
- 1993-12-21 LT LTIP1656A patent/LT4029B/lt not_active IP Right Cessation
-
1994
- 1994-07-01 HU HU94P/P00552P patent/HU212071A9/hu unknown
-
1995
- 1995-06-29 HU HU95P/P00552P patent/HU00552A9/hu unknown
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU96105702A (ru) | ПОСЛЕДОВАТЕЛЬНОСТЬ ДНК, ТРАНСФОРМИРОВАННАЯ КЛЕТКА, СПОСОБ ПОЛУЧЕНИЯ РЕКОМБИНАНТНОЙ ПЕПТИДИЛГЛИЦИНα-АМИДИРУЮЩЕЙ МОНООКСИГЕНАЗЫ И СПОСОБ ПОЛУЧЕНИЯ АМИДИРОВАННЫХ ПЕПТИДОВ | |
| RU2004126135A (ru) | Новый цитокин zalpha11-лиганд | |
| ES2009238A6 (es) | Metodo para producir proteinas de mycobacterium tuberculosis y peptidos antigenicos de las mismas. | |
| RU2002124123A (ru) | Модифицированный фактор viii | |
| NO885659D0 (no) | Mycobakterielle rekombinanter og peptider. | |
| DE3583824D1 (de) | Vorlaeufer des insulins, verfahren zu seiner herstellung und verfahren zur herstellung von humaninsulin aus solchen vorlaeufern des insulins. | |
| EP0150126A3 (en) | The synthesis of protein with an identification peptide | |
| DK1218751T3 (da) | Cöliakidiagnose under anvendelse af et gliadinepitop | |
| RU99107670A (ru) | Высокопродуктивная экспрессия белков | |
| KR880014110A (ko) | 인체 프로아포지방단백질 a-i의 발현 | |
| IL94128A (en) | Nucleic acids encoding vascular endothelial cell growth factors, expression vectors and host cells comprising such nucleic acids and methods of producing such vascular endothelial growth factors | |
| ES8708015A1 (es) | Un procedimiento para preparar relaxina -h2 humana o uno de sus analogos | |
| NZ502391A (en) | Mammalian cytokine: interleukin-B30 and related reagents | |
| ES8802160A1 (es) | Un procedimiento para aislar un gen de relaxina cromosomica de un ser humano o de otro primate o una especie afin | |
| Katsoyannis | The synthesis of the insulin chains and their combination to biologically active material | |
| KR900016261A (ko) | 독사독 폴리펩티드 및 유전적 발현 | |
| KR880009991A (ko) | 항혈액응고작용을 갖는 폴리펩티드 | |
| EP0236978A3 (en) | Production of factor xiiia by gene technology | |
| CA2390049A1 (en) | Control of post-transcriptional gene silencing in plants | |
| NZ320932A (en) | Short forms of chemokine b-8 | |
| ES8405842A1 (es) | Procedimiento de producir razas estables de celulas de mamiferos | |
| DK217285A (da) | Signalpeptid til udskillelse af peptider i streptomyceter | |
| EP0249412A3 (en) | C-terminal alpha-amidating enzyme and process for production and use thereof | |
| KR910016923A (ko) | 트롬빈 결합성 물질의 제조법 | |
| EP0086649A3 (en) | Molecular cloning and characterization of the gene sequence coding for porcine relaxin |