RU2746407C1 - Хроматографическое выделение клеток и других сложных биологических материалов - Google Patents
Хроматографическое выделение клеток и других сложных биологических материалов Download PDFInfo
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- RU2746407C1 RU2746407C1 RU2014138172A RU2014138172A RU2746407C1 RU 2746407 C1 RU2746407 C1 RU 2746407C1 RU 2014138172 A RU2014138172 A RU 2014138172A RU 2014138172 A RU2014138172 A RU 2014138172A RU 2746407 C1 RU2746407 C1 RU 2746407C1
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Families Citing this family (66)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9637719B2 (en) | 2013-12-06 | 2017-05-02 | Douglas T. Gjerde | Devices and methods for purification of biological cells |
| RU2746407C1 (ru) | 2012-02-23 | 2021-04-13 | Стейдж Селл Терапеутикс Гмбх | Хроматографическое выделение клеток и других сложных биологических материалов |
| PT3083671T (pt) | 2013-12-20 | 2020-12-24 | Hutchinson Fred Cancer Res | Moléculas efetoras quiméricas etiquetadas e recetores destas |
| EP3126027A4 (en) * | 2014-04-03 | 2017-11-01 | Douglas T. Gjerde | Devices and methods for purification, detection and use of biological cells |
| IL293587B2 (en) | 2014-04-16 | 2025-02-01 | Juno Therapeutics Gmbh | Methods, kits and devices for increasing cell populations |
| CN106459917B (zh) * | 2014-04-23 | 2021-03-09 | 朱诺治疗学股份有限公司 | 分离、培养和遗传工程改造用于过继治疗的免疫细胞群的方法 |
| JP6678593B2 (ja) * | 2014-04-30 | 2020-04-08 | イーベーアー ゲーエムベーハー | 標的細胞を単離する方法 |
| EP2955521A1 (en) * | 2014-06-11 | 2015-12-16 | Centre Hospitalier Universitaire Vaudois (CHUV) | Methods for separating cells |
| HUE050264T2 (hu) * | 2014-11-05 | 2020-11-30 | Juno Therapeutics Inc | Eljárások transzdukálásra és sejtfeldolgozásra |
| DE202014105965U1 (de) | 2014-12-10 | 2015-01-26 | Cell.Copedia GmbH | Spritzengehäuse zum Pipettieren eines biologischen Materials mit integrierten Membranen |
| WO2016166568A1 (en) | 2015-04-16 | 2016-10-20 | Juno Therapeutics Gmbh | Methods, kits and apparatus for expanding a population of cells |
| US11827904B2 (en) | 2015-04-29 | 2023-11-28 | Fred Hutchinson Cancer Center | Modified stem cells and uses thereof |
| CN105606815A (zh) * | 2015-05-18 | 2016-05-25 | 烟台载通生物技术有限公司 | 一种用于免疫检测的多单元装置 |
| MA45426A (fr) | 2015-10-22 | 2019-05-01 | Juno Therapeutics Gmbh | Procédés, kits, agents et appareils de transduction |
| MA45488A (fr) | 2015-10-22 | 2018-08-29 | Juno Therapeutics Gmbh | Procédés, kits et appareil de culture de cellules |
| MA45489A (fr) * | 2015-10-22 | 2018-08-29 | Juno Therapeutics Gmbh | Procédés de culture de cellules, kits et appareil associés |
| GB201521784D0 (en) * | 2015-12-10 | 2016-01-27 | Univ Birmingham | Cell purification |
| JP7062640B2 (ja) | 2016-07-29 | 2022-05-06 | ジュノー セラピューティクス インコーポレイテッド | 抗ｃｄ１９抗体に対する抗イディオタイプ抗体 |
| US10294454B2 (en) | 2016-08-24 | 2019-05-21 | General Electric Company | Methods and kits for cell activation |
| JP7376091B2 (ja) | 2016-12-23 | 2023-11-08 | エクソファーマ リミテッド | 微小胞およびエキソソームの精製または単離のための方法および組成物 |
| MX2019008538A (es) | 2017-01-20 | 2019-11-05 | Juno Therapeutics Gmbh | Conjugados de superficie celular y composiciones y métodos celulares relacionados. |
| CN111032850B (zh) * | 2017-04-27 | 2024-08-13 | 朱诺治疗学有限公司 | 寡聚粒子试剂及其使用方法 |
| KR20200064060A (ko) | 2017-07-29 | 2020-06-05 | 주노 쎄러퓨티크스 인코퍼레이티드 | 재조합 수용체를 발현하는 세포 증폭용 시약 |
| MA49979A (fr) | 2017-08-09 | 2020-06-17 | Juno Therapeutics Inc | Procédés de production de compositions de cellules génétiquement modifiées et compositions associées |
| EP3704230B1 (en) | 2017-11-01 | 2024-10-23 | Juno Therapeutics, Inc. | Process for generating therapeutic compositions of engineered cells |
| CN112004824B (zh) | 2017-12-08 | 2024-09-13 | 朱诺治疗学股份有限公司 | 生产工程化t细胞组合物的过程 |
| WO2019133842A1 (en) * | 2017-12-29 | 2019-07-04 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Purification and labeling of extracellular vesicles using a mixed mode resin composition |
| CN119881293A (zh) * | 2018-03-21 | 2025-04-25 | 沃特世科技公司 | 基于非抗体高亲和力的样品制备、吸附剂、装置和方法 |
| BR112020026067A2 (pt) | 2018-06-18 | 2021-03-23 | Exopharm Limited | métodos e composições para purificação ou isolamento de microvesículas e exossomas |
| JP2021530230A (ja) * | 2018-07-17 | 2021-11-11 | ユニヴァーシティ オブ ワシントン | アプタマーベースの可逆的細胞選択に関連する組成物および方法 |
| CN113474450A (zh) | 2018-08-09 | 2021-10-01 | 朱诺治疗学股份有限公司 | 产生工程化细胞的方法以及所述工程化细胞的组合物 |
| US12366580B2 (en) | 2018-09-11 | 2025-07-22 | Juno Therapeutics, Inc. | Methods for mass spectrometry analysis of engineered cell compositions |
| EP3874024A1 (en) | 2018-10-31 | 2021-09-08 | Juno Therapeutics GmbH | Methods for selection and stimulation of cells and apparatus for same |
| WO2020097132A1 (en) | 2018-11-06 | 2020-05-14 | Juno Therapeutics, Inc. | Process for producing genetically engineered t cells |
| BR112021024404A2 (pt) | 2019-06-07 | 2022-04-19 | Juno Therapeutics Inc | Cultura de célula t automatizada |
| US12194221B2 (en) * | 2019-06-27 | 2025-01-14 | Bioimmunate Technologies Ltd. | Filter for removal of multiple sclerosis-associated t-cells |
| EP4022637A2 (en) | 2019-08-30 | 2022-07-06 | Juno Therapeutics, Inc. | Machine learning methods for classifying cells |
| BR112022002969A2 (pt) * | 2019-08-30 | 2022-05-17 | Flodesign Sonics Inc | Seleção de células de afinidade acústica para múltiplos receptores alvo |
| JP2022547309A (ja) * | 2019-09-10 | 2022-11-11 | ピアス・バイオテクノロジー・インコーポレイテッド | サンプル調製組成物、デバイス、システムおよび方法 |
| WO2021084050A1 (en) | 2019-10-30 | 2021-05-06 | Juno Therapeutics Gmbh | Cell selection and/or stimulation devices and methods of use |
| WO2021118873A1 (en) * | 2019-12-09 | 2021-06-17 | St. Jude Children's Research Hospital, Inc. | Method for preparing cd7-negative, cd3-positive t cells |
| AU2021214142A1 (en) | 2020-01-28 | 2022-09-08 | Juno Therapeutics, Inc. | Methods for T cell transduction |
| AU2021213439B2 (en) * | 2020-01-31 | 2023-01-05 | Cell.Copedia GmbH | Methods of isolating a biological entity |
| KR20220152220A (ko) | 2020-02-12 | 2022-11-15 | 주노 쎄러퓨티크스 인코퍼레이티드 | Ｃｄ19-지시된 키메라 항원 수용체 ｔ 세포 조성물 및 이의 방법 및 용도 |
| EP4103203A1 (en) | 2020-02-12 | 2022-12-21 | Juno Therapeutics, Inc. | Bcma-directed chimeric antigen receptor t cell compositions and methods and uses thereof |
| US20230181641A1 (en) | 2020-05-13 | 2023-06-15 | Juno Therapeutics, Inc. | Process for producing donor-batched cells expressing a recombinant receptor |
| EP4334341A2 (en) | 2021-05-06 | 2024-03-13 | Juno Therapeutics GmbH | Methods for stimulating and transducing t cells |
| WO2023017115A1 (en) * | 2021-08-11 | 2023-02-16 | Iba Lifesciences Gmbh | Fractionation of cells based on a marker protein |
| CN113908589B (zh) * | 2021-10-08 | 2022-09-27 | 天津工业大学 | 一种表面印迹抗体的疏水电荷诱导模式膜层析介质及其制备方法 |
| WO2023147510A1 (en) | 2022-01-28 | 2023-08-03 | Juno Therapeutics, Inc. | Chromatography arrays and related methods and kits |
| ES3024941T3 (en) * | 2022-02-02 | 2025-06-05 | Sartorius Bia Separations D O O | Method of purifying or removing a target component from full plasma |
| WO2023213969A1 (en) | 2022-05-05 | 2023-11-09 | Juno Therapeutics Gmbh | Viral-binding protein and related reagents, articles, and methods of use |
| EP4532695A1 (en) | 2022-05-25 | 2025-04-09 | Celgene Corporation | Methods of manufacturing t cell therapies |
| WO2024100604A1 (en) | 2022-11-09 | 2024-05-16 | Juno Therapeutics Gmbh | Methods for manufacturing engineered immune cells |
| CN121100267A (zh) | 2022-12-09 | 2025-12-09 | 朱诺治疗学股份有限公司 | 使用全息成像预测细胞表型的机器学习方法 |
| CN121002052A (zh) | 2023-02-03 | 2025-11-21 | C3S2 有限公司 | 用于非病毒生产工程化免疫细胞的方法 |
| CN121127253A (zh) | 2023-02-28 | 2025-12-12 | 朱诺治疗学股份有限公司 | 治疗系统性自身免疫性疾病的细胞疗法 |
| WO2025049254A1 (en) | 2023-08-25 | 2025-03-06 | Juno Therapeutics, Inc. | Pyrazolopyridine based inhibitors of dna-dependent protein kinase and compositions and application in gene editing |
| WO2025049250A1 (en) | 2023-08-25 | 2025-03-06 | Juno Therapeutics, Inc. | 6,6-core silacycle based inhibitors of dna-dependent protein kinase and compositions and application in gene editing |
| WO2025049253A1 (en) | 2023-08-25 | 2025-03-06 | Juno Therapeutics, Inc. | Bridged cycle‑based inhibitors of dna‑dependent protein kinase and compositions and application in gene editing |
| WO2025049247A1 (en) | 2023-08-25 | 2025-03-06 | Juno Therapeutics, Inc. | 5,6-core silacycle based inhibitors of dna-dependent protein kinase and compositions and application in gene editing |
| WO2025083196A1 (en) | 2023-10-18 | 2025-04-24 | Tq Therapeutics Gmbh | A method of manufacturing a cellular therapy product and respective kits and devices |
| US12434218B2 (en) | 2023-11-28 | 2025-10-07 | Phenomenex, Inc. | Pore structure for separation of adeno-associated viruses (AAVS) from their aggregates |
| US20250241912A1 (en) | 2024-01-25 | 2025-07-31 | Juno Therapeutics, Inc. | Use of bridged cycle-based inhibitors of dna-dependent protein kinase in combination of dna polymerase theta inhibitor and compositions and application in gene editing |
| WO2025179188A1 (en) | 2024-02-22 | 2025-08-28 | Juno Therapeutics, Inc. | Pyrazole-based inhibitors of dna-dependent protein kinase and compositions and applications in gene editing |
| US20250345431A1 (en) | 2024-05-10 | 2025-11-13 | Juno Therapeutics, Inc. | Genetically engineered t cells expressing a cd19 chimeric antigen receptor (car) and uses thereof for allogeneic cell therapy |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002054065A2 (en) * | 2000-12-28 | 2002-07-11 | Iba Gmbh | Reversible mhc multimer staining for functional purification of antigen-specific t cells |
| RU2402777C2 (ru) * | 2004-10-08 | 2010-10-27 | Аффитек Ас | Способ скрининга библиотеки фагового дисплея |
Family Cites Families (166)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2467434A (en) | 1947-05-10 | 1949-04-19 | Air Associates Inc | Servomotor and pressure responsive valve therefor |
| US4235871A (en) | 1978-02-24 | 1980-11-25 | Papahadjopoulos Demetrios P | Method of encapsulating biologically active materials in lipid vesicles |
| US4361549A (en) | 1979-04-26 | 1982-11-30 | Ortho Pharmaceutical Corporation | Complement-fixing monoclonal antibody to human T cells, and methods of preparing same |
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| JP2624470B2 (ja) | 1984-10-02 | 1997-06-25 | バイオジェン インコーポレイテッド | ストレプトアビジン様ポリペプチドの製造 |
| US4851341A (en) | 1986-12-19 | 1989-07-25 | Immunex Corporation | Immunoaffinity purification system |
| US6534055B1 (en) | 1988-11-23 | 2003-03-18 | Genetics Institute, Inc. | Methods for selectively stimulating proliferation of T cells |
| US6352694B1 (en) | 1994-06-03 | 2002-03-05 | Genetics Institute, Inc. | Methods for inducing a population of T cells to proliferate using agents which recognize TCR/CD3 and ligands which stimulate an accessory molecule on the surface of the T cells |
| GB9215540D0 (en) | 1992-07-22 | 1992-09-02 | Celltech Ltd | Protein expression system |
| DE4237113B4 (de) | 1992-11-03 | 2006-10-12 | "Iba Gmbh" | Peptide und deren Fusionsproteine, Expressionsvektor und Verfahren zur Herstellung eines Fusionsproteins |
| DE4417598A1 (de) | 1994-05-19 | 1995-12-14 | Max Planck Gesellschaft | Verwendung des Tetracyclinpromotors zur stringent regulierten Produktion von rekombinanten Proteinen in prokaryontischen Zellen |
| US7074904B2 (en) | 1994-07-29 | 2006-07-11 | Altor Bioscience Corporation | MHC complexes and uses thereof |
| DK0776339T4 (da) | 1994-07-29 | 2010-05-25 | Sunol Molecular Corp | MHC-komplekser og anvendelser deraf |
| AU5132096A (en) | 1995-01-30 | 1996-08-21 | Terrapin Technologies, Inc. | Glubodies - multiplicities of proteins capable of binding a variety of small molecules |
| CA2211951A1 (en) | 1995-02-09 | 1996-08-15 | University Of Washington | Modified-affinity streptavidin |
| WO1997011183A1 (en) | 1995-04-11 | 1997-03-27 | Trustees Of Boston University | Streptavidin mutants |
| US5629205A (en) | 1995-05-19 | 1997-05-13 | Allelix Biopharmaceuticals Inc. | Promoters for gene expression |
| WO1997005266A1 (en) | 1995-07-25 | 1997-02-13 | Introgene B.V. | Methods and means for targeted gene delivery |
| AR005035A1 (es) | 1995-12-11 | 1999-04-07 | Merck Patent Ges Mit Beschränkter Haftung | Procedimiento para preparar proteinas recombinantes en e. coli, mediante fermentacion con gran concentracion de celulas. |
| US5869270A (en) | 1996-01-31 | 1999-02-09 | Sunol Molecular Corporation | Single chain MHC complexes and uses thereof |
| US5773224A (en) | 1996-02-12 | 1998-06-30 | Grandics; Peter | Immunoselection system for cell elution |
| DE19637718A1 (de) | 1996-04-01 | 1997-10-02 | Boehringer Mannheim Gmbh | Rekombinante inaktive Core-Streptavidin Mutanten |
| US6022688A (en) | 1996-05-13 | 2000-02-08 | Sequenom, Inc. | Method for dissociating biotin complexes |
| US20050003431A1 (en) | 1996-08-16 | 2005-01-06 | Wucherpfennig Kai W. | Monovalent, multivalent, and multimeric MHC binding domain fusion proteins and conjugates, and uses therefor |
| AU730457B2 (en) | 1996-08-16 | 2001-03-08 | President And Fellows Of Harvard College | Soluble monovalent and multivalent MHC class II fusion proteins, and uses therefor |
| DE19641876B4 (de) | 1996-10-10 | 2011-09-29 | Iba Gmbh | Streptavidinmuteine |
| US6368813B1 (en) | 1997-03-14 | 2002-04-09 | The Trustees Of Boston University | Multiflavor streptavidin |
| US6391571B1 (en) | 1997-04-01 | 2002-05-21 | Roche Diagnostics Gmbh | Recombinant inactive avidin mutants |
| CA2302779C (en) | 1997-09-16 | 2010-02-02 | Oregon Health Sciences University | Recombinant mhc molecules useful for manipulation of antigen-specific t-cells |
| US6232445B1 (en) | 1997-10-29 | 2001-05-15 | Sunol Molecular Corporation | Soluble MHC complexes and methods of use thereof |
| US5985658A (en) | 1997-11-14 | 1999-11-16 | Health Research Incorporated | Calmodulin-based cell separation technique |
| NZ506071A (en) | 1998-02-19 | 2003-05-30 | Harvard College | Monovalent, multivalent, and multimeric major histocompatibility complex (MHC) binding domain fusion proteins and conjugates, and uses therefore |
| US6664042B1 (en) | 1999-01-26 | 2003-12-16 | Cornell Research Foundation, Inc. | Determining viral load in double negative T cells |
| KR20020013496A (ko) | 1999-02-04 | 2002-02-20 | 추후제출 | 조혈 간세포 및(또는) 전구세포의 유지 및 증대 방법 및장치 |
| EP1048732A1 (de) | 1999-04-26 | 2000-11-02 | F. Hoffmann-La Roche Ag | Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen |
| EP1669129A1 (en) | 1999-05-14 | 2006-06-14 | Pall Corporation | Charged membrane |
| WO2000069549A1 (en) | 1999-05-14 | 2000-11-23 | Pall Corporation | Charged membrane |
| US6849185B1 (en) | 1999-05-14 | 2005-02-01 | Pall Corp | Charged membrane |
| DE60043889D1 (en) | 1999-06-07 | 2010-04-08 | Tet Systems Holding Gmbh & Co | Tet repressor basierte transkriptionale regulatorische proteine |
| DE19932688B4 (de) | 1999-07-13 | 2009-10-08 | Scil Proteins Gmbh | Design von Beta-Faltblatt-Proteinen des gamma-II-kristallins antikörperähnlichen |
| US20030235908A1 (en) | 2000-02-24 | 2003-12-25 | Xcyte Therapies, Inc. | Activation and expansion of cells |
| DE60127113T2 (de) | 2000-04-25 | 2007-06-14 | Otsuka Pharmaceutical Co., Ltd. | Gd3-mimetische peptide |
| US6638728B1 (en) | 2000-06-16 | 2003-10-28 | Pierce Biotechnology, Inc. | Coated surfaces with high capacity for capturing target molecules |
| JP4878724B2 (ja) | 2000-11-03 | 2012-02-15 | ジェネンテック， インコーポレイテッド | 組み換えタンパク質を発現する発酵における代謝速度シフト |
| US6979556B2 (en) | 2000-12-14 | 2005-12-27 | Genentech, Inc. | Separate-cistron contructs for secretion of aglycosylated antibodies from prokaryotes |
| EP1349569B1 (en) | 2001-01-12 | 2007-04-18 | Becton Dickinson and Company | Intrinsically fluorescent, self-multimerizing mhc fusion proteins and complexes thereof |
| GB0102568D0 (en) | 2001-02-01 | 2001-03-21 | Magnetic Biosolutions Sweden A | Method |
| DE10113776B4 (de) | 2001-03-21 | 2012-08-09 | "Iba Gmbh" | Isoliertes streptavidinbindendes, kompetitiv eluierbares Peptid, dieses umfassendes Fusionspeptid, dafür codierende Nukleinsäure, Expressionsvektor, Verfahren zur Herstellung eines rekombinanten Fusionsproteins und Verfahren zum Nachweis und/oder zur Gewinnung des Fusionsproteins |
| EP1908769A1 (en) | 2001-08-27 | 2008-04-09 | Genentech, Inc. | A system for antibody expression and assembly |
| KR100968664B1 (ko) | 2001-08-27 | 2010-07-06 | 제넨테크, 인크. | 항체 발현 및 조립을 위한 시스템 |
| WO2003029462A1 (en) | 2001-09-27 | 2003-04-10 | Pieris Proteolab Ag | Muteins of human neutrophil gelatinase-associated lipocalin and related proteins |
| US7094579B2 (en) | 2002-02-13 | 2006-08-22 | Xoma Technology Ltd. | Eukaryotic signal sequences for prokaryotic expression |
| JP2005536184A (ja) * | 2002-03-01 | 2005-12-02 | エルドマン，ヴォルカー，エー． | ストレプトアビジン結合ペプチド |
| US7754155B2 (en) * | 2002-03-15 | 2010-07-13 | Ross Amelia A | Devices and methods for isolating target cells |
| WO2003090781A1 (en) | 2002-04-23 | 2003-11-06 | Meir Strahilevitz | Methods and devices for targeting a site in a mammal and for removing species from a mammal |
| ATE345502T1 (de) | 2002-06-24 | 2006-12-15 | Profos Ag | Verfahren zum nachweis und zur entfernung von endotoxin |
| FR2841905B1 (fr) | 2002-07-05 | 2004-09-03 | Centre Nat Rech Scient | Fragments fab mutants de l'anticorps anti-cd4 chimere 13b8.2 et leurs applications |
| CN102061288A (zh) | 2002-07-17 | 2011-05-18 | 希托斯生物技术股份公司 | 使用衍生自ap205外壳蛋白的病毒样颗粒的分子抗原阵列 |
| GB2392158B (en) | 2002-08-21 | 2005-02-16 | Proimmune Ltd | Chimeric MHC protein and oligomer thereof |
| EP1569510B1 (en) | 2002-09-27 | 2011-11-02 | The General Hospital Corporation | Microfluidic device for cell separation and uses thereof |
| EP1588166A4 (en) | 2003-01-09 | 2007-06-27 | Macrogenics Inc | VECTOR SYSTEM WITH DOUBLE EXPRESSION FOR EXPRESSING ANTIBODIES IN BACTERIAL AND MAMMAL CELLS |
| WO2004096975A2 (en) | 2003-05-02 | 2004-11-11 | Insception Bioscience, Inc. | Apparatus and methods for amplification of blood stem cell numbers |
| EP1623017B1 (en) | 2003-05-08 | 2010-09-15 | Life Technologies Corporation | Generation and isolation of antigen-specific t cells |
| US7943393B2 (en) * | 2003-07-14 | 2011-05-17 | Phynexus, Inc. | Method and device for extracting an analyte |
| ES2391457T3 (es) | 2003-08-13 | 2012-11-26 | Sandoz Ag | Procedimiento para la purificación de polipéptidos recombinantes |
| GB0319601D0 (en) | 2003-08-20 | 2003-09-24 | Sandoz Ag | Production process |
| GB0321100D0 (en) | 2003-09-09 | 2003-10-08 | Celltech R&D Ltd | Biological products |
| GB2408507B (en) | 2003-10-06 | 2005-12-14 | Proimmune Ltd | Chimeric MHC protein and oligomer thereof for specific targeting |
| KR100570422B1 (ko) | 2003-10-16 | 2006-04-11 | 한미약품 주식회사 | 대장균 분비서열을 이용하여 항체 단편을 분비·생산하는 발현벡터 및 이를 이용하여 항체 단편을 대량 생산하는 방법 |
| AU2004292131A1 (en) | 2003-11-20 | 2005-06-02 | Biosensor Applications Sweden (Publ) | Mixture of at least two different antibodies specific for predetermined antigens and use of the mixture |
| BRPI0416807A (pt) | 2003-11-21 | 2007-01-09 | Dow Global Technologies Inc | sistemas de expressão aperfeiçoados com secreção de sistema sec |
| SE0400181D0 (sv) | 2004-01-29 | 2004-01-29 | Gyros Ab | Segmented porous and preloaded microscale devices |
| US7592431B2 (en) | 2004-02-26 | 2009-09-22 | Immunovative Therapies, Ltd. | Biodegradable T-cell Activation device |
| DK1733037T3 (en) | 2004-03-11 | 2015-01-12 | Genentech Inc | Process for the preparation of polypeptides |
| WO2005121798A1 (en) * | 2004-06-03 | 2005-12-22 | Meso Scale Technologies, Llc | Methods and apparatuses for conducting assays |
| US20110098184A1 (en) | 2004-10-15 | 2011-04-28 | Day Anthony G | Competitve differntial screeing |
| WO2006058226A2 (en) | 2004-11-24 | 2006-06-01 | The Trustees Of Boston University | Modified dimeric streptavidins and uses thereof |
| US20060252087A1 (en) | 2005-01-18 | 2006-11-09 | Biocept, Inc. | Recovery of rare cells using a microchannel apparatus with patterned posts |
| RU2441067C2 (ru) | 2005-09-28 | 2012-01-27 | Цитос Биотехнологи Аг | Конъюгаты интерлейкина-1 и их применение |
| US7704708B2 (en) | 2006-02-13 | 2010-04-27 | Uti Limited Partnership | Monomeric streptavidin muteins |
| US7855057B2 (en) * | 2006-03-23 | 2010-12-21 | Millipore Corporation | Protein splice variant/isoform discrimination and quantitative measurements thereof |
| US20090208471A1 (en) | 2006-04-07 | 2009-08-20 | Yun Theodore J | Isolation and Use of Human Regulatory T Cells |
| US20070238169A1 (en) | 2006-04-11 | 2007-10-11 | The Board Of Trustees Of The Leland Stanford Junior University | Cell sorter and culture system |
| GB2442048B (en) | 2006-07-25 | 2009-09-30 | Proimmune Ltd | Biotinylated MHC complexes and their uses |
| US20080085532A1 (en) | 2006-09-18 | 2008-04-10 | Jorn Gorlach | Method for determining the immune status of a subject |
| DE102006044841A1 (de) | 2006-09-22 | 2008-04-03 | Wacker Chemie Ag | Signalpeptid zur Produktion von rekombinanten Proteinen |
| DE502006006800D1 (de) | 2006-09-22 | 2010-06-02 | Wacker Chemie Ag | Verfahren zur fermentativen Herstellung von Proteinen |
| DE502006009060D1 (de) | 2006-09-22 | 2011-04-21 | Wacker Chemie Ag | Verfahren zur fermentativen Herstellung von Antikörpern |
| EP1905839B2 (de) | 2006-09-22 | 2019-07-10 | Wacker Chemie AG | Verfahren zur fermentativen Herstellung von Proteinen |
| KR101454287B1 (ko) | 2006-10-17 | 2014-11-04 | 온코세라피 사이언스 가부시키가이샤 | Ｍｐｈｏｓｐｈ1 또는 ｄｅｐｄｃ1 폴리펩티드를 발현하는 암에 대한 펩티드 백신 |
| US8043822B2 (en) * | 2006-11-02 | 2011-10-25 | Kyowa Medex Co., Ltd. | Method of immunoassaying a component to be measured |
| CA2671326A1 (en) * | 2006-11-15 | 2008-11-20 | Invitrogen Dynal As | Methods of reversibly binding a biotin compound to a support |
| US20080131415A1 (en) | 2006-11-30 | 2008-06-05 | Riddell Stanley R | Adoptive transfer of cd8 + t cell clones derived from central memory cells |
| CN101226118B (zh) * | 2007-01-19 | 2010-06-16 | 中国医学科学院肿瘤研究所 | 一种兼容免疫荧光分析的细胞化学染色方法及其用途 |
| CN102517280A (zh) | 2007-01-31 | 2012-06-27 | 菲尼克斯股份有限公司 | 用于提高表达的细菌前导序列 |
| KR20080076622A (ko) | 2007-02-16 | 2008-08-20 | 포항공과대학교 산학협력단 | 올리고머화된 단백질 전달체와 이를 이용한 세포내바이러스 벡터 전달 방법 |
| WO2008118476A2 (en) | 2007-03-26 | 2008-10-02 | Codon Devices, Inc. | Cell surface display, screening and production of proteins of interest |
| EP2155782A2 (en) | 2007-03-26 | 2010-02-24 | Dako Denmark A/S | Mhc peptide complexes and uses thereof in infectious diseases |
| EP3620465B1 (en) | 2007-07-03 | 2025-02-19 | Dako Denmark A/S | Improved methods for generation, labeling and use of mhc multimers |
| US8735330B2 (en) | 2007-08-20 | 2014-05-27 | Nextera As | pVII phage display |
| JP4962215B2 (ja) | 2007-08-28 | 2012-06-27 | パナソニック株式会社 | 墨出し器の精度確認装置及び墨出し器の精度確認方法 |
| ES2319061B1 (es) | 2007-09-11 | 2010-02-10 | Biomedal, S.L. | Metodo de conservacion de peptidos o proteinas. |
| US10611818B2 (en) | 2007-09-27 | 2020-04-07 | Agilent Technologies, Inc. | MHC multimers in tuberculosis diagnostics, vaccine and therapeutics |
| EP2433713B1 (en) | 2007-12-07 | 2017-07-26 | Miltenyi Biotec GmbH | Cell processing systems and methods |
| CA2712303C (en) * | 2008-01-18 | 2015-03-31 | President And Fellows Of Harvard College | Methods of detecting signatures of disease or conditions in bodily fluids |
| CA2713462C (en) | 2008-01-29 | 2020-04-14 | Fred Hutchinson Cancer Research Center | Identification of cd8+ t cells that are cd161hi and/or il18r(alpha)hi and have rapid drug efflux capacity |
| SG172737A1 (en) | 2008-01-30 | 2011-07-28 | Pieris Ag | Muteins of tear lipocalin having affinity to human c-met receptor tyrosine kinaseand methods for obtaining the same |
| EP2254592B1 (en) | 2008-02-28 | 2019-06-05 | Dako Denmark A/S | Mhc multimers in borrelia diagnostics and disease |
| WO2010037395A2 (en) | 2008-10-01 | 2010-04-08 | Dako Denmark A/S | Mhc multimers in cancer vaccines and immune monitoring |
| CN101446576B (zh) | 2008-12-29 | 2011-06-22 | 江苏省苏微微生物研究有限公司 | 一种微囊藻毒素-lr单抗免疫亲和柱的制备和使用方法 |
| WO2010080032A2 (en) | 2009-01-09 | 2010-07-15 | Stichting Het Nederlands Kanker Instituut | Bead-assisted viral transduction |
| DE102009018647A1 (de) | 2009-04-23 | 2010-10-28 | Osram Opto Semiconductors Gmbh | Strahlungsemittierende Vorrichtung |
| US20110070581A1 (en) | 2009-04-27 | 2011-03-24 | Amit Gupta | Separation of Leukocytes |
| US20120214187A1 (en) | 2009-11-02 | 2012-08-23 | Ffina Biolutions, Llc | Method for Enhancing the Sensitivity of Antibody Based Assays |
| KR101968766B1 (ko) | 2009-11-05 | 2019-04-12 | 제넨테크, 인크. | 이종 폴리펩티드의 분비를 위한 방법 및 조성물 |
| MY159680A (en) | 2009-11-17 | 2017-01-13 | Janssen Biotech Inc | Improved bacterial membrane protein secrection |
| US20120321665A1 (en) | 2009-12-14 | 2012-12-20 | Benaroya Research Institute At Virginia Mason | Compositions and methods for treating airway inflammatory diseases |
| US9242244B2 (en) | 2010-02-09 | 2016-01-26 | Douglas T. Gjerde | Method and apparatus for pipette tip columns |
| US9370732B2 (en) | 2013-02-15 | 2016-06-21 | Douglas T. Gjerde | Methods for purifying biological cells |
| US9637719B2 (en) | 2013-12-06 | 2017-05-02 | Douglas T. Gjerde | Devices and methods for purification of biological cells |
| US9891148B2 (en) | 2010-02-09 | 2018-02-13 | Douglas T. Gjerde | Method and apparatus for pipette tip columns |
| GB201002730D0 (en) | 2010-02-18 | 2010-04-07 | Uni I Oslo | Product |
| EP2363501A1 (en) * | 2010-03-02 | 2011-09-07 | Universitätsklinikum Hamburg-Eppendorf | Method for isolating target cells |
| JP5665021B2 (ja) | 2010-03-08 | 2015-02-04 | 国立大学法人東京農工大学 | 融合ｍｈｃ分子連結磁気微粒子、抗原ペプチドのスクリーニング方法、組換えベクター、及び磁性細菌の形質転換体 |
| WO2011161088A2 (en) | 2010-06-23 | 2011-12-29 | Stobbe Tech. A/S | Biopharmaceutical process apparatuses assembled into a column |
| GB201012603D0 (en) | 2010-07-27 | 2010-09-08 | Ucb Pharma Sa | Protein purification |
| US9410157B2 (en) | 2010-07-30 | 2016-08-09 | Wisconsin Alumni Research Foundation | Systems and methods for the secretion of recombinant proteins in gram negative bacteria |
| US9678061B2 (en) * | 2010-08-06 | 2017-06-13 | Ludwig-Maximilians-Universität München | Identification of T cell target antigens |
| WO2012044999A2 (en) | 2010-10-01 | 2012-04-05 | Ludwig Institute For Cancer Research Ltd. | Reversible protein multimers, methods for their production and use |
| WO2012058627A2 (en) | 2010-10-29 | 2012-05-03 | Miqin Zhang | Pre-targeted nanoparticle system and method for labeling biological particles |
| MX359513B (es) | 2011-03-23 | 2018-10-01 | Hutchinson Fred Cancer Res | Metodo y composiciones para inmunoterapia celular. |
| JP5840857B2 (ja) | 2011-04-08 | 2016-01-06 | 国立大学法人 東京大学 | 細胞傷害性ｔ細胞誘導用組成物 |
| CN103797028B (zh) | 2011-07-18 | 2017-08-25 | Iba 股份有限公司 | 使靶细胞可逆染色的方法 |
| US9353161B2 (en) | 2011-09-13 | 2016-05-31 | Uti Limited Partnership | Streptavidin mutein exhibiting reversible binding for biotin and streptavidin binding peptide tagged proteins |
| WO2013062365A2 (ko) | 2011-10-26 | 2013-05-02 | 국립암센터 | 변이 ｃｔｌａ4 유전자 이입 ｔ 세포 및 이를 포함하는 항암 면역치료용 조성물 |
| RU2746407C1 (ru) * | 2012-02-23 | 2021-04-13 | Стейдж Селл Терапеутикс Гмбх | Хроматографическое выделение клеток и других сложных биологических материалов |
| UA114108C2 (uk) | 2012-07-10 | 2017-04-25 | Борд Оф Ріджентс, Дзе Юніверсіті Оф Техас Сістем | Моноклональне антитіло для застосування в діагностиці і терапії злоякісних пухлин і аутоімунного захворювання |
| EP3584256A1 (en) | 2012-07-13 | 2019-12-25 | The Trustees Of The University Of Pennsylvania | Methods of assessing the suitability of transduced t cells for administration |
| IL293944A (en) | 2012-08-20 | 2022-08-01 | Hutchinson Fred Cancer Res | Method and preparations for cellular immunotherapy |
| EP2711418B1 (en) | 2012-09-25 | 2017-08-23 | Miltenyi Biotec GmbH | Method for polyclonal stimulation of T cells by flexible nanomatrices |
| US10065996B2 (en) | 2012-11-16 | 2018-09-04 | Iba Gmbh | Streptavidin muteins and methods of using them |
| KR20150113105A (ko) | 2013-01-31 | 2015-10-07 | 글락소 그룹 리미티드 | 단백질을 생성하는 방법 |
| US10107729B2 (en) | 2013-02-15 | 2018-10-23 | Douglas T. Gjerde | Isolation, detection and use of biological cells |
| US9920294B2 (en) | 2013-02-15 | 2018-03-20 | Douglas T. Gjerde | Devices and methods for purification, detection and use of biological cells |
| US10220332B2 (en) | 2013-02-15 | 2019-03-05 | Douglas T. Gjerde | Columns for isolation, detection and use of biological cells |
| CN103305464B (zh) | 2013-06-05 | 2015-04-15 | 南昌大学 | 直接分离cd4+和cd8+淋巴细胞的方法 |
| PT3083671T (pt) | 2013-12-20 | 2020-12-24 | Hutchinson Fred Cancer Res | Moléculas efetoras quiméricas etiquetadas e recetores destas |
| IL293587B2 (en) | 2014-04-16 | 2025-02-01 | Juno Therapeutics Gmbh | Methods, kits and devices for increasing cell populations |
| CN106459917B (zh) | 2014-04-23 | 2021-03-09 | 朱诺治疗学股份有限公司 | 分离、培养和遗传工程改造用于过继治疗的免疫细胞群的方法 |
| ES2708584T3 (es) | 2014-04-24 | 2019-04-10 | Miltenyi Biotec Gmbh | Método para la generación automatizada de células T modificadas genéticamente |
| RS60544B1 (sr) | 2014-04-25 | 2020-08-31 | Bluebird Bio Inc | Poboljšani metodi za proizvodnju adoptivnih ćelijskih terapija |
| JP6678593B2 (ja) | 2014-04-30 | 2020-04-08 | イーベーアー ゲーエムベーハー | 標的細胞を単離する方法 |
| EP2955196A1 (en) | 2014-06-10 | 2015-12-16 | Effimune | Antibodies directed against CD127 |
| EP3172228B1 (en) | 2014-07-25 | 2019-02-27 | Theravectys | Lentiviral vectors for regulated expression of a chimeric antigen receptor molecule |
| GB201415344D0 (en) | 2014-08-29 | 2014-10-15 | Ucl Business Plc | Protein |
| WO2016166568A1 (en) | 2015-04-16 | 2016-10-20 | Juno Therapeutics Gmbh | Methods, kits and apparatus for expanding a population of cells |
| MA45426A (fr) | 2015-10-22 | 2019-05-01 | Juno Therapeutics Gmbh | Procédés, kits, agents et appareils de transduction |
| MA45488A (fr) | 2015-10-22 | 2018-08-29 | Juno Therapeutics Gmbh | Procédés, kits et appareil de culture de cellules |
| MA45489A (fr) | 2015-10-22 | 2018-08-29 | Juno Therapeutics Gmbh | Procédés de culture de cellules, kits et appareil associés |
| AU2016363025B2 (en) | 2015-12-03 | 2021-04-08 | Juno Therapeutics, Inc. | Modified chimeric receptors and related compositions and methods |
| JP2019510503A (ja) | 2016-04-07 | 2019-04-18 | ブルーバード バイオ， インコーポレイテッド | キメラ抗原受容体ｔ細胞組成物 |
| MX2019008538A (es) | 2017-01-20 | 2019-11-05 | Juno Therapeutics Gmbh | Conjugados de superficie celular y composiciones y métodos celulares relacionados. |
| CN111032850B (zh) | 2017-04-27 | 2024-08-13 | 朱诺治疗学有限公司 | 寡聚粒子试剂及其使用方法 |
| US20190247846A1 (en) | 2018-02-09 | 2019-08-15 | Chris Suh | Method and Apparatus for Pipette Tip Columns |
| CN113474450A (zh) | 2018-08-09 | 2021-10-01 | 朱诺治疗学股份有限公司 | 产生工程化细胞的方法以及所述工程化细胞的组合物 |
| EP3874024A1 (en) | 2018-10-31 | 2021-09-08 | Juno Therapeutics GmbH | Methods for selection and stimulation of cells and apparatus for same |
-
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002054065A2 (en) * | 2000-12-28 | 2002-07-11 | Iba Gmbh | Reversible mhc multimer staining for functional purification of antigen-specific t cells |
| RU2402777C2 (ru) * | 2004-10-08 | 2010-10-27 | Аффитек Ас | Способ скрининга библиотеки фагового дисплея |
Non-Patent Citations (7)
| Title |
|---|
| ASHOK K. et al. Affinity binding of cells to cryogel adsorbents with immobilized specific ligands: effect of ligand coupling and matrix architecture. Journal of molecular recognition. 2005, V.18, P. 84-93, стр.84-87, 89-90. * |
| ASHOK K. et al. Affinity binding of cells to cryogel adsorbents with immobilized specific ligands: effect of ligand coupling and matrix architecture. Journal of molecular recognition. 2005, V.18, P. 84-93, стр.84-87, 89-90. WANG K. et al. Open-tubular capillary cell affinity chromatography: single and tandem blood cell separation. Analytical chemistry. 2008, V. 80, P.2118-2124, стр.2118-2120, 2124, фиг.1. XU YE et al. Aptamer-based microfluidic device for enrichment, sorting, and detection of multiple cancer cells. Analytical chemistry. 2009, V.81, P. 7436-7442, стр.7436, 7438-7440, фиг.1. CASALEGNO-GARDUNO R. et al. Multimer technologies for detection and adoptive transfer of antigen-specific T cells. Cancer immunology, immunotherapy. 2009, V.59, P.195-202, стр.196, фиг.1-2. SCHMITT A. et al. Adoptive transfer and selective reconstitution of streptamer-selected cytomegalovirus-specific CD8+ T-cells leads to virus clearance in patients after allogeneic peripheral blood stem cell transp * |
| CASALEGNO-GARDUNO R. et al. Multimer technologies for detection and adoptive transfer of antigen-specific T cells. Cancer immunology, immunotherapy. 2009, V.59, P.195-202, стр.196, фиг.1-2. * |
| MILTENYI S. et al. High gradient magnetic cell seperation with MACS. Cytometry. 1990, V.11, P.231-238, весь текст. * |
| SCHMITT A. et al. Adoptive transfer and selective reconstitution of streptamer-selected cytomegalovirus-specific CD8+ T-cells leads to virus clearance in patients after allogeneic peripheral blood stem cell transplantation. Transfusion. 2010, V. 51, P.591-599, стр.592. * |
| WANG K. et al. Open-tubular capillary cell affinity chromatography: single and tandem blood cell separation. Analytical chemistry. 2008, V. 80, P.2118-2124, стр.2118-2120, 2124, фиг.1. * |
| XU YE et al. Aptamer-based microfluidic device for enrichment, sorting, and detection of multiple cancer cells. Analytical chemistry. 2009, V.81, P. 7436-7442, стр.7436, 7438-7440, фиг.1. * |
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