RU2633644C2 - Способы ферментативного синтеза могрозидных соединений - Google Patents

Способы ферментативного синтеза могрозидных соединений Download PDF

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RU2633644C2
RU2633644C2 RU2014125216A RU2014125216A RU2633644C2 RU 2633644 C2 RU2633644 C2 RU 2633644C2 RU 2014125216 A RU2014125216 A RU 2014125216A RU 2014125216 A RU2014125216 A RU 2014125216A RU 2633644 C2 RU2633644 C2 RU 2633644C2
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mogroside
recombinant
mogroside compound
mogrol
polypeptide
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RU2014125216A
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English (en)
Russian (ru)
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RU2014125216A (ru
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Яоцюань Лю
Дзунг Йеоп ЛИ
Моника КХАРЕ
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Эвольва Са
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/20—Preparation of steroids containing heterocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
RU2014125216A 2011-11-23 2012-11-19 Способы ферментативного синтеза могрозидных соединений RU2633644C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161563303P 2011-11-23 2011-11-23
US61/563,303 2011-11-23
PCT/IB2012/002857 WO2013076577A1 (en) 2011-11-23 2012-11-19 Methods and materials for enzymatic synthesis of mogroside compounds

Publications (2)

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RU2014125216A RU2014125216A (ru) 2015-12-27
RU2633644C2 true RU2633644C2 (ru) 2017-10-16

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RU2014125216A RU2633644C2 (ru) 2011-11-23 2012-11-19 Способы ферментативного синтеза могрозидных соединений

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US (2) US9920349B2 (OSRAM)
EP (1) EP2783009B1 (OSRAM)
JP (1) JP6084983B2 (OSRAM)
KR (1) KR101791597B1 (OSRAM)
CN (1) CN103958693B (OSRAM)
AU (2) AU2012342114B2 (OSRAM)
BR (1) BR112014012543A2 (OSRAM)
CA (1) CA2853677C (OSRAM)
IN (1) IN2014CN03557A (OSRAM)
MX (1) MX342851B (OSRAM)
MY (1) MY170686A (OSRAM)
RU (1) RU2633644C2 (OSRAM)
SG (1) SG11201401927PA (OSRAM)
WO (1) WO2013076577A1 (OSRAM)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102181638B1 (ko) 2010-06-02 2020-11-24 에볼바, 인크. 스테비올 글리코시드의 재조합 생산
EP3792350A1 (en) 2011-08-08 2021-03-17 Evolva SA Recombinant production of steviol glycosides
SG11201401927PA (en) 2011-11-23 2014-09-26 Evolva Sa Methods and materials for enzymatic synthesis of mogroside compounds
TW201402599A (zh) * 2012-05-30 2014-01-16 Suntory Holdings Ltd 甜菊糖苷糖苷轉化酵素及編碼該酵素之基因
BR112015013129A2 (pt) 2012-12-04 2017-07-11 Evolva Sa métodos e materiais para a biossíntese de compostos mogrosida
WO2016050890A2 (en) 2014-10-01 2016-04-07 Evolva Sa Methods and materials for biosynthesis of mogroside compounds
MX2015010098A (es) 2013-02-11 2016-04-19 Evolva Sa Producción eficiente de glicosidos de esteviol en huéspedes recombinantes.
SG11201700651RA (en) 2014-08-11 2017-02-27 Evolva Sa Production of steviol glycosides in recombinant hosts
MX2017003130A (es) 2014-09-09 2017-10-24 Evolva Sa Produccion de glucosidos de esteviol en hospederos recombinantes.
CA2972739A1 (en) 2014-09-11 2016-03-17 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Methods of producing mogrosides and compositions comprising same and uses thereof
JP6698028B2 (ja) * 2014-10-17 2020-05-27 サントリーホールディングス株式会社 モグロール配糖体化酵素およびそれをコードする遺伝子
JP6850130B2 (ja) 2015-01-20 2021-03-31 サントリーホールディングス株式会社 モグロシドの調製方法
US10364450B2 (en) 2015-01-30 2019-07-30 Evolva Sa Production of steviol glycoside in recombinant hosts
EP3271457B1 (en) 2015-03-16 2021-01-20 DSM IP Assets B.V. Udp-glycosyltransferases
JP2018522569A (ja) 2015-08-07 2018-08-16 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． 組換え宿主内でのステビオールグリコシドの産生
SG11201803603WA (en) 2015-10-29 2018-05-30 Senomyx Inc High intensity sweeteners
CN106924274B (zh) * 2015-12-29 2019-01-04 成都普睿法药物研发有限公司 葫芦烷型四环三萜类化合物抗肺纤维化应用
BR112018071144B1 (pt) 2016-04-13 2023-04-18 Evolva Sa Célula hospedeira recombinante que produz um ou mais glicosídeos de esteviol ou uma composição de glicosídeo de esteviol em uma cultura de células e métodos para produzir um ou mais glicosídeos de esteviol ou uma composição de glicosídeo de esteviol, bem como cultura de células e lisado celular da dita célula hospedeira recombinante
AU2017267214A1 (en) * 2016-05-16 2018-11-15 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3458599A1 (en) 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
WO2018016483A1 (ja) * 2016-07-19 2018-01-25 サントリーホールディングス株式会社 モグロールまたはモグロール配糖体の生産方法
CN110100006A (zh) 2016-11-07 2019-08-06 埃沃尔瓦公司 重组宿主中甜菊糖苷的生产
MX2019012940A (es) 2017-05-03 2019-12-16 Firmenich Incorporated Metodos para producir edulcorantes de alta intensidad.
US11653680B2 (en) 2017-05-19 2023-05-23 The Coca-Cola Company Siamenoside I sweetened compositions and uses of the same
WO2021214008A1 (en) 2020-04-20 2021-10-28 Givaudan Sa Compositions
RU2762719C2 (ru) 2017-06-02 2021-12-22 Живодан Са Композиция, модифицирующая сладость
WO2018229283A1 (en) 2017-06-15 2018-12-20 Evolva Sa Production of mogroside compounds in recombinant hosts
CN107189976B (zh) * 2017-07-28 2022-03-11 李华政 促进罗汉果ugt72b21基因表达的方法
WO2020096905A1 (en) 2018-11-07 2020-05-14 Firmenich Incorporated Methods for making high intensity sweeteners
KR20210089717A (ko) 2018-11-07 2021-07-16 피르메니히 인코포레이티드 고강도 감미료의 제조 방법
WO2020097588A1 (en) 2018-11-09 2020-05-14 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
CN110669094B (zh) * 2019-10-14 2021-12-21 上海交通大学 罗汉果新苷及其制备方法
KR102362135B1 (ko) * 2019-10-25 2022-02-14 고려대학교 산학협력단 테르펜 당쇄 부가 유도체, 이의 제조방법 및 이를 유효성분으로 함유하는 조성물
WO2021188703A1 (en) * 2020-03-17 2021-09-23 The Coca-Cola Company Novel mogroside production system and methods
GB202007984D0 (en) 2020-05-28 2020-07-15 Givaudan Sa Compositions
CN112063647B (zh) * 2020-09-17 2023-05-02 云南农业大学 酿酒酵母重组菌Cuol01的构建方法、酿酒酵母重组菌Cuol02及应用
CN116064712B (zh) * 2022-10-08 2025-05-06 中国药科大学 一种罗汉果醇的生物合成方法
CN121127603A (zh) 2023-04-20 2025-12-12 帝斯曼知识产权资产管理有限公司 用于产生罗汉果苷的菌株和方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001012845A1 (en) * 1999-08-18 2001-02-22 National Research Council Of Canada Screening for udp-glucose: glycoprotein glucosyltransferase (uggt) activity
RU2008123244A (ru) * 2005-11-23 2009-12-27 Дзе Кока-Кола Компани (US) Композиция интенсивного подсластителя с глюкозамином и подслащенные ею композиции

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1510573A1 (en) * 2003-09-01 2005-03-02 Austria Wirtschaftsservice Gesellschaft mit beschränkter Haftung Method for detoxification of mycotoxins
US20080148432A1 (en) * 2005-12-21 2008-06-19 Mark Scott Abad Transgenic plants with enhanced agronomic traits
CA2598792A1 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
US8257948B1 (en) * 2011-02-17 2012-09-04 Purecircle Usa Method of preparing alpha-glucosyl Stevia composition
US8956677B2 (en) 2005-11-23 2015-02-17 The Coca-Cola Company High-potency sweetener composition with glucosamine and compositions sweetened therewith
US7927851B2 (en) 2006-03-21 2011-04-19 Vineland Research And Innovation Centre Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same
US20100143975A1 (en) * 2006-11-22 2010-06-10 The University Of York Monoterpenoid modifying enzymes
US20100132073A1 (en) * 2006-12-01 2010-05-27 The University Of York Sesquiterpenoid modifying enzymes
GB0904639D0 (en) * 2009-03-18 2009-04-29 Univ York Assay
KR102181638B1 (ko) 2010-06-02 2020-11-24 에볼바, 인크. 스테비올 글리코시드의 재조합 생산
SG11201401927PA (en) 2011-11-23 2014-09-26 Evolva Sa Methods and materials for enzymatic synthesis of mogroside compounds
BR112015013129A2 (pt) 2012-12-04 2017-07-11 Evolva Sa métodos e materiais para a biossíntese de compostos mogrosida

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001012845A1 (en) * 1999-08-18 2001-02-22 National Research Council Of Canada Screening for udp-glucose: glycoprotein glucosyltransferase (uggt) activity
RU2008123244A (ru) * 2005-11-23 2009-12-27 Дзе Кока-Кола Компани (US) Композиция интенсивного подсластителя с глюкозамином и подслащенные ею композиции

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
POPPENBERGER B. et al. "Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana", Journal of Biological Chemistry, 2003, v.278, no.48, p.47905-47914. *
RICHMAN A. et al. "Functional genomics uncovers three glucosyltransferases involved in the synthesis of the major sweet glucosides of Stevia rebaudiana", The Plant Journal, 2005, v.41, p.56-67. *
RICHMAN A. et al. "Functional genomics uncovers three glucosyltransferases involved in the synthesis of the major sweet glucosides of Stevia rebaudiana", The Plant Journal, 2005, v.41, p.56-67. TANG Q. et al. "An efficient approach to finding Siraitia grosvenorii triterpene biosynthetic genes by RNA-seq and digital gene expression analysis", BMC GENOMICS, 05.07.2011, v.12, no.343, p.1-13. SHAO Hui et al. "Crystal structures of a multifunctional triterpene/flavonoid glycosyltransferase from Medicago truncatula", The Plant Cell, 2005, v.17, no.11, p.3141-3154. *
SHAO Hui et al. "Crystal structures of a multifunctional triterpene/flavonoid glycosyltransferase from Medicago truncatula", The Plant Cell, 2005, v.17, no.11, p.3141-3154. *
TANG Q. et al. "An efficient approach to finding Siraitia grosvenorii triterpene biosynthetic genes by RNA-seq and digital gene expression analysis", BMC GENOMICS, 05.07.2011, v.12, no.343, p.1-13. *

Also Published As

Publication number Publication date
MY170686A (en) 2019-08-26
AU2012342114A1 (en) 2014-05-22
KR101791597B1 (ko) 2017-10-30
MX2014006276A (es) 2014-10-13
RU2014125216A (ru) 2015-12-27
CN103958693B (zh) 2016-08-24
CA2853677A1 (en) 2013-05-30
US20180237819A1 (en) 2018-08-23
JP6084983B2 (ja) 2017-02-22
KR20140099281A (ko) 2014-08-11
EP2783009A1 (en) 2014-10-01
CN103958693A (zh) 2014-07-30
AU2017235907A1 (en) 2017-10-12
MX342851B (es) 2016-10-14
WO2013076577A1 (en) 2013-05-30
SG11201401927PA (en) 2014-09-26
AU2012342114B2 (en) 2017-12-21
HK1200494A1 (zh) 2015-08-07
US20140308698A1 (en) 2014-10-16
CA2853677C (en) 2019-01-29
US10738340B2 (en) 2020-08-11
BR112014012543A2 (pt) 2017-06-13
JP2014533518A (ja) 2014-12-15
IN2014CN03557A (OSRAM) 2015-10-09
US9920349B2 (en) 2018-03-20
EP2783009B1 (en) 2016-04-13

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Effective date: 20191120