RU2564569C2 - Промывочный раствор и способ промывки для аффинной хроматографии - Google Patents

Промывочный раствор и способ промывки для аффинной хроматографии Download PDF

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Publication number
RU2564569C2
RU2564569C2 RU2012130151/10A RU2012130151A RU2564569C2 RU 2564569 C2 RU2564569 C2 RU 2564569C2 RU 2012130151/10 A RU2012130151/10 A RU 2012130151/10A RU 2012130151 A RU2012130151 A RU 2012130151A RU 2564569 C2 RU2564569 C2 RU 2564569C2
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RU
Russia
Prior art keywords
protein
arginine
washing
concentration
antibody
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RU2012130151/10A
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English (en)
Russian (ru)
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RU2012130151A (ru
Inventor
ФРАУЕНШУХ Ахим
БИЛЛЬ Курт
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Новартис Аг
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Publication of RU2012130151A publication Critical patent/RU2012130151A/ru
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
RU2012130151/10A 2009-12-18 2010-12-17 Промывочный раствор и способ промывки для аффинной хроматографии RU2564569C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28805909P 2009-12-18 2009-12-18
US61/288,059 2009-12-18
PCT/EP2010/070076 WO2011073389A1 (en) 2009-12-18 2010-12-17 Wash solution and method for affinity chromatography

Publications (2)

Publication Number Publication Date
RU2012130151A RU2012130151A (ru) 2014-01-27
RU2564569C2 true RU2564569C2 (ru) 2015-10-10

Family

ID=43598315

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2012130151/10A RU2564569C2 (ru) 2009-12-18 2010-12-17 Промывочный раствор и способ промывки для аффинной хроматографии

Country Status (18)

Country Link
US (3) US9505803B2 (https=)
EP (2) EP2513134B1 (https=)
JP (2) JP5909450B2 (https=)
KR (1) KR101858266B1 (https=)
CN (1) CN102725304B (https=)
AU (1) AU2010332778B2 (https=)
BR (1) BR112012014937B1 (https=)
CA (1) CA2784278C (https=)
DK (2) DK2513134T3 (https=)
ES (2) ES2651065T3 (https=)
IL (1) IL220164B (https=)
LT (2) LT2513134T (https=)
MX (1) MX2012007103A (https=)
PL (2) PL3272764T3 (https=)
PT (2) PT2513134T (https=)
RU (1) RU2564569C2 (https=)
SG (2) SG10201408384PA (https=)
WO (1) WO2011073389A1 (https=)

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US12528835B2 (en) 2017-12-21 2026-01-20 Genzyme Corporation Methods for enhanced removal of impurities during protein a chromatography

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CN111902720B (zh) 2018-03-21 2025-02-07 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
AU2019246983B2 (en) 2018-04-02 2025-12-18 Amgen Inc. Erenumab compositions and uses thereof
CN112105927B (zh) * 2018-05-08 2024-06-25 沃特世科技公司 可用于pH梯度阳离子交换色谱法的方法、组合物和试剂盒
KR102893280B1 (ko) * 2018-08-14 2025-12-01 브리스톨-마이어스 스큅 컴퍼니 개선된 단백질 회수
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CN112827217A (zh) * 2020-12-29 2021-05-25 苏州良辰生物医药科技有限公司 一种层析柱清洗液及其应用
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2773852C2 (ru) * 2017-12-21 2022-06-14 Джензим Корпорейшн Способы улучшенного удаления примесей при проведении хроматографии на основе связывания с белком а
US12528835B2 (en) 2017-12-21 2026-01-20 Genzyme Corporation Methods for enhanced removal of impurities during protein a chromatography

Also Published As

Publication number Publication date
LT2513134T (lt) 2017-11-27
SG181771A1 (en) 2012-07-30
SG10201408384PA (en) 2015-01-29
KR20120109506A (ko) 2012-10-08
IL220164A0 (en) 2012-07-31
JP2016128495A (ja) 2016-07-14
AU2010332778A1 (en) 2012-07-19
EP2513134A1 (en) 2012-10-24
BR112012014937B1 (pt) 2021-01-19
US20120283416A1 (en) 2012-11-08
LT3272764T (lt) 2024-05-10
CN102725304A (zh) 2012-10-10
US9505803B2 (en) 2016-11-29
JP2013514073A (ja) 2013-04-25
EP3272764B1 (en) 2024-02-07
PL3272764T3 (pl) 2024-06-10
MX2012007103A (es) 2012-07-17
PT2513134T (pt) 2017-12-14
JP5909450B2 (ja) 2016-04-26
CA2784278C (en) 2019-02-26
DK3272764T3 (da) 2024-05-06
PL2513134T3 (pl) 2018-02-28
PT3272764T (pt) 2024-04-24
ES2651065T3 (es) 2018-01-24
CA2784278A1 (en) 2011-06-23
AU2010332778B2 (en) 2015-03-19
KR101858266B1 (ko) 2018-05-15
US20170044211A1 (en) 2017-02-16
ES2982297T3 (es) 2024-10-15
CN102725304B (zh) 2015-12-09
DK2513134T3 (en) 2017-12-18
US20190233469A1 (en) 2019-08-01
RU2012130151A (ru) 2014-01-27
BR112012014937A2 (pt) 2016-03-08
IL220164B (en) 2019-02-28
EP3272764A1 (en) 2018-01-24
EP2513134B1 (en) 2017-09-06
WO2011073389A1 (en) 2011-06-23

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