KR101858266B1 - 친화성 크로마토그래피를 위한 세척용액 및 방법 - Google Patents

친화성 크로마토그래피를 위한 세척용액 및 방법 Download PDF

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Publication number
KR101858266B1
KR101858266B1 KR1020127015524A KR20127015524A KR101858266B1 KR 101858266 B1 KR101858266 B1 KR 101858266B1 KR 1020127015524 A KR1020127015524 A KR 1020127015524A KR 20127015524 A KR20127015524 A KR 20127015524A KR 101858266 B1 KR101858266 B1 KR 101858266B1
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arginine
protein
column
matrix
wash
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KR20120109506A (ko
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아힘 프라우엔슈
쿠르트 빌
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노파르티스 아게
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020127015524A 2009-12-18 2010-12-17 친화성 크로마토그래피를 위한 세척용액 및 방법 Active KR101858266B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28805909P 2009-12-18 2009-12-18
US61/288,059 2009-12-18
PCT/EP2010/070076 WO2011073389A1 (en) 2009-12-18 2010-12-17 Wash solution and method for affinity chromatography

Publications (2)

Publication Number Publication Date
KR20120109506A KR20120109506A (ko) 2012-10-08
KR101858266B1 true KR101858266B1 (ko) 2018-05-15

Family

ID=43598315

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KR1020127015524A Active KR101858266B1 (ko) 2009-12-18 2010-12-17 친화성 크로마토그래피를 위한 세척용액 및 방법

Country Status (18)

Country Link
US (3) US9505803B2 (https=)
EP (2) EP2513134B1 (https=)
JP (2) JP5909450B2 (https=)
KR (1) KR101858266B1 (https=)
CN (1) CN102725304B (https=)
AU (1) AU2010332778B2 (https=)
BR (1) BR112012014937B1 (https=)
CA (1) CA2784278C (https=)
DK (2) DK3272764T3 (https=)
ES (2) ES2982297T3 (https=)
IL (1) IL220164B (https=)
LT (2) LT2513134T (https=)
MX (1) MX2012007103A (https=)
PL (2) PL2513134T3 (https=)
PT (2) PT3272764T (https=)
RU (1) RU2564569C2 (https=)
SG (2) SG181771A1 (https=)
WO (1) WO2011073389A1 (https=)

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EP3337818B1 (en) * 2015-08-21 2026-03-18 F. Hoffmann-La Roche AG Affinity chromatography purification with low conductivity wash buffer
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US10513537B2 (en) 2016-05-11 2019-12-24 Ge Healthcare Bioprocess R&D Ab Separation matrix
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US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
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CN107793469B (zh) * 2017-10-16 2021-07-30 上海药明生物技术有限公司 一种去除宿主细胞蛋白含量的亲和纯化工艺
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WO2019183334A1 (en) 2018-03-21 2019-09-26 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
JP7840636B2 (ja) 2018-04-02 2026-04-06 アムジェン インコーポレイテッド エレヌマブ組成物及びその使用
WO2019217570A1 (en) * 2018-05-08 2019-11-14 Waters Technologies Corporation Methods, compositions and kits useful for ph gradient cation exchange chromatography
US12103949B2 (en) * 2018-08-14 2024-10-01 Bristol-Myers Squibb Company Protein recovery
CN114502569A (zh) * 2019-10-08 2022-05-13 北卡罗莱纳州立大学 免疫球蛋白纯化肽及其用途
WO2021191684A1 (en) * 2020-03-27 2021-09-30 Haemalogix Pty Ltd Composition and method
WO2021222120A1 (en) * 2020-04-27 2021-11-04 Alexion Pharmaceuticals, Inc. Protein a chromatography purification of an antibody
CN112827217A (zh) * 2020-12-29 2021-05-25 苏州良辰生物医药科技有限公司 一种层析柱清洗液及其应用
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WO2024233992A2 (en) * 2023-05-11 2024-11-14 Trophogen, Inc. Methods of manufacturing highly glycosylated glycoprotein hormones and their analogs
EP4729534A1 (en) 2024-10-21 2026-04-22 Technische Universität München, in Vertretung des Freistaats Bayern Polypeptides comprising protein domains linked via rigid amino acid linkers

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SG10201408384PA (en) 2015-01-29
US20190233469A1 (en) 2019-08-01
US9505803B2 (en) 2016-11-29
JP2016128495A (ja) 2016-07-14
CN102725304B (zh) 2015-12-09
EP2513134A1 (en) 2012-10-24
WO2011073389A1 (en) 2011-06-23
BR112012014937A2 (pt) 2016-03-08
PT3272764T (pt) 2024-04-24
ES2982297T3 (es) 2024-10-15
PL3272764T3 (pl) 2024-06-10
IL220164B (en) 2019-02-28
CA2784278A1 (en) 2011-06-23
US20170044211A1 (en) 2017-02-16
AU2010332778B2 (en) 2015-03-19
DK3272764T3 (da) 2024-05-06
PL2513134T3 (pl) 2018-02-28
EP2513134B1 (en) 2017-09-06
ES2651065T3 (es) 2018-01-24
JP5909450B2 (ja) 2016-04-26
DK2513134T3 (en) 2017-12-18
CN102725304A (zh) 2012-10-10
LT2513134T (lt) 2017-11-27
IL220164A0 (en) 2012-07-31
KR20120109506A (ko) 2012-10-08
RU2012130151A (ru) 2014-01-27
BR112012014937B1 (pt) 2021-01-19
RU2564569C2 (ru) 2015-10-10
LT3272764T (lt) 2024-05-10
EP3272764B1 (en) 2024-02-07
CA2784278C (en) 2019-02-26
SG181771A1 (en) 2012-07-30
US20120283416A1 (en) 2012-11-08
JP2013514073A (ja) 2013-04-25
EP3272764A1 (en) 2018-01-24
AU2010332778A1 (en) 2012-07-19
MX2012007103A (es) 2012-07-17
PT2513134T (pt) 2017-12-14

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