RU2532228C2 - Композиции и способы, используемые для оценки цитотоксичности отдельных клеток - Google Patents

Композиции и способы, используемые для оценки цитотоксичности отдельных клеток Download PDF

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RU2532228C2
RU2532228C2 RU2011134898/10A RU2011134898A RU2532228C2 RU 2532228 C2 RU2532228 C2 RU 2532228C2 RU 2011134898/10 A RU2011134898/10 A RU 2011134898/10A RU 2011134898 A RU2011134898 A RU 2011134898A RU 2532228 C2 RU2532228 C2 RU 2532228C2
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cells
cell
effector
hiv
target
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RU2011134898/10A
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RU2011134898A (ru
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Джон Кристофер ЛАВ
Навин ВАРАДАРАДЖАН
Борис ЮЛЬГ
Брюс УОЛКЕР
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Массачусетс Инститьют Оф Текнолоджи
ДЗЕ ДЖЕНЕРАЛ ХОСПИТАЛ КОРПОРЕЙШН д/б/а МАССАЧУСЕТС ДЖЕНЕРАЛ ХОСПИТАЛ
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/06—Methods of screening libraries by measuring effects on living organisms, tissues or cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16—HIV-1, HIV-2

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
RU2011134898/10A 2009-01-21 2009-07-13 Композиции и способы, используемые для оценки цитотоксичности отдельных клеток RU2532228C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14610609P 2009-01-21 2009-01-21
US61/146,106 2009-01-21
PCT/US2009/050411 WO2010085275A1 (en) 2009-01-21 2009-07-13 Compositions and methods for assessing cytotoxicity of single cells

Publications (2)

Publication Number Publication Date
RU2011134898A RU2011134898A (ru) 2013-02-27
RU2532228C2 true RU2532228C2 (ru) 2014-10-27

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US (1) US9244071B2 (enExample)
EP (1) EP2389446B1 (enExample)
JP (2) JP2012515548A (enExample)
KR (1) KR20110134386A (enExample)
CN (2) CN102388145A (enExample)
AU (1) AU2009338127B2 (enExample)
CA (1) CA2689681C (enExample)
IL (1) IL213938A0 (enExample)
RU (1) RU2532228C2 (enExample)
SG (2) SG172889A1 (enExample)
WO (1) WO2010085275A1 (enExample)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9404924B2 (en) 2008-12-04 2016-08-02 Massachusetts Institute Of Technology Method of performing one-step, single cell RT-PCR
DK2370814T3 (da) 2008-12-04 2015-07-20 Massachusetts Inst Technology Fremgangsmåde til diagnosticering af allergiske reaktioner
CN102388145A (zh) 2009-01-21 2012-03-21 麻省理工学院 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法
WO2012072823A1 (en) * 2010-12-03 2012-06-07 Mindseeds Laboratories Srl Rapid screening of monoclonal antibodies
US9891157B2 (en) 2010-12-03 2018-02-13 Cellply S.R.L. Microanalysis of cellular function
WO2013090404A2 (en) * 2011-12-13 2013-06-20 Single Cell Technology, Inc. Method of screening a plurality of single secreting cells for functional activity
US20150191772A1 (en) 2012-06-29 2015-07-09 Danmarks Tekniske Universitet Method of charging a test carrier and a test carrier
CN104884605B (zh) * 2012-08-24 2018-05-18 耶鲁大学 用于高通量多重检测的系统、装置和方法
WO2015031190A1 (en) * 2013-08-26 2015-03-05 President And Fellows Of Harvard College Determination of immune cells and other cells
US10508280B2 (en) 2014-01-31 2019-12-17 AgBiome, Inc. Modified biological control agents and their uses
US9877486B2 (en) 2014-01-31 2018-01-30 AgBiome, Inc. Methods of growing plants using modified biological control agents
EP3227684B1 (en) 2014-12-03 2019-10-02 Isoplexis Corporation Analysis and screening of cell secretion profiles
WO2016149639A1 (en) 2015-03-19 2016-09-22 The Board Of Trustees Of The Leland Stanford Junior University Devices and methods for high-throughput single cell and biomolecule analysis and retrieval in a microfluidic chip
US10746736B2 (en) 2015-03-26 2020-08-18 University Of Houston System Integrated functional and molecular profiling of cells
US12071663B2 (en) 2016-01-15 2024-08-27 Massachusetts Institute Of Technology Semi-permeable arrays for analyzing biological systems and methods of using same
US12345708B2 (en) 2016-03-25 2025-07-01 President And Fellows Of Harvard College Microfluidic determination of immune and other cells
WO2017169267A1 (ja) * 2016-03-30 2017-10-05 ソニー株式会社 細胞観察装置、免疫細胞の活性度の評価方法及び免疫細胞の品質管理方法
WO2017216739A1 (en) 2016-06-14 2017-12-21 Cellply S.R.L. Screening kit and method
WO2017216270A1 (de) 2016-06-15 2017-12-21 Grabmayr Heinrich Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie
CN106086171A (zh) * 2016-06-15 2016-11-09 广州中大南沙科技创新产业园有限公司 一种抑制肿瘤细胞增殖的药物筛选方法
US20200166518A1 (en) 2016-09-12 2020-05-28 IsoPlexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
EP3532600A4 (en) * 2016-10-19 2020-09-16 General Automation LAB Technologies Inc. HIGH RESOLUTION SYSTEMS, KITS, DEVICES AND METHODS FOR SCREENING MICRO-ORGANISMS AND OTHER HIGH-PERFORMANCE MICROBIOLOGICAL APPLICATIONS
US11493508B2 (en) * 2016-11-11 2022-11-08 IsoPlexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
US11085912B2 (en) * 2016-12-14 2021-08-10 University Of Cincinnati Methods of diagnosing Clostridium difficile infection or recurrence in a subject
SE543211C2 (en) * 2017-06-29 2020-10-27 Mabtech Production Ab Method and system for analyzing Fluorospot assays
WO2019113457A1 (en) 2017-12-07 2019-06-13 Massachusetts Institute Of Technology Single cell analyses
CN108728356B (zh) * 2018-05-23 2022-02-22 北京科技大学 用于不同三维细胞团配对的装置及共培养方法
CN113614532A (zh) * 2019-01-16 2021-11-05 烟台澳斯邦生物工程有限公司 沉积用于显微镜检查的生物样品的自动液体处理系统和方法
CA3130051A1 (en) * 2019-02-18 2020-08-27 Nb Health Laboratory Co., Ltd. Method for selecting cells, method for producing nucleic acid, method for producing recombinant cells, method for producing target substance, method for producing pharmaceutical composition, and reagent
US12064768B2 (en) 2020-02-21 2024-08-20 Northeastern University Single cell isolation and processing system with reversible well shape
CN115176158A (zh) 2020-02-27 2022-10-11 伊索普莱克西斯公司 用于多路分析的系统、装置及方法
WO2024102720A2 (en) 2022-11-07 2024-05-16 Shennon Biotechnologies Inc. Microfluidic devices for high throughput screening of cell-cell interactions
WO2026042829A1 (ja) * 2024-08-21 2026-02-26 ジェノコンシェルジュ京都株式会社 Ｋｉｒ遺伝子のｐｃｒ増幅法およびロングリードシークエンサーを用いた増幅産物のシークエンス法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1650094A1 (ru) * 1987-12-29 1991-05-23 Научно-исследовательский институт проктологии Способ диагностики прот женности активного воспалени в толстой кишке при неспецифическом звенном колите
WO2000061629A1 (en) * 1999-04-09 2000-10-19 Human Genome Sciences, Inc. 49 human secreted proteins
WO2000077026A1 (en) * 1999-06-11 2000-12-21 Human Genome Sciences, Inc. 49 human secreted proteins

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL68507A (en) 1982-05-10 1986-01-31 Univ Bar Ilan System and methods for cell selection
US6410252B1 (en) 1995-12-22 2002-06-25 Case Western Reserve University Methods for measuring T cell cytokines
US6210910B1 (en) 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
US7332286B2 (en) * 2001-02-02 2008-02-19 University Of Pennsylvania Peptide or protein microassay method and apparatus
US20020141906A1 (en) 2001-03-30 2002-10-03 American Type Culture Collection Microtiter plate sealing cover with well identifiers
US7776553B2 (en) 2005-09-16 2010-08-17 Presidents And Fellows Of Harvard College Screening assays and methods
CN102112624A (zh) 2008-05-30 2011-06-29 麻省理工学院 用于对细胞进行空间分割以及筛选的组合物以及方法
DK2370814T3 (da) 2008-12-04 2015-07-20 Massachusetts Inst Technology Fremgangsmåde til diagnosticering af allergiske reaktioner
US9404924B2 (en) 2008-12-04 2016-08-02 Massachusetts Institute Of Technology Method of performing one-step, single cell RT-PCR
CN102388145A (zh) 2009-01-21 2012-03-21 麻省理工学院 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法
US8569046B2 (en) 2009-02-20 2013-10-29 Massachusetts Institute Of Technology Microarray with microchannels

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1650094A1 (ru) * 1987-12-29 1991-05-23 Научно-исследовательский институт проктологии Способ диагностики прот женности активного воспалени в толстой кишке при неспецифическом звенном колите
WO2000061629A1 (en) * 1999-04-09 2000-10-19 Human Genome Sciences, Inc. 49 human secreted proteins
WO2000077026A1 (en) * 1999-06-11 2000-12-21 Human Genome Sciences, Inc. 49 human secreted proteins

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
RENAT SHAYKHIEV and ROBERT BALS. Interactions between epithelial cells and leukocytes in immunity and tissue homeostasis.July 2007. Journal of Leukocyte Biology. vol. 82 no. 1 1-15 *

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EP2389446B1 (en) 2017-07-05
CN102388145A (zh) 2012-03-21
CN104745669A (zh) 2015-07-01
IL213938A0 (en) 2011-07-31
SG172889A1 (en) 2011-08-29
SG10201401643PA (en) 2014-08-28
CA2689681A1 (en) 2010-07-21
US20120149592A1 (en) 2012-06-14
JP2015144617A (ja) 2015-08-13
US9244071B2 (en) 2016-01-26
AU2009338127B2 (en) 2015-11-26
KR20110134386A (ko) 2011-12-14
AU2009338127A1 (en) 2011-07-28
JP6017620B2 (ja) 2016-11-02
CA2689681C (en) 2017-08-29
WO2010085275A1 (en) 2010-07-29
JP2012515548A (ja) 2012-07-12
EP2389446A4 (en) 2012-10-03
EP2389446A1 (en) 2011-11-30
RU2011134898A (ru) 2013-02-27

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