CN102388145A - 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法 - Google Patents

用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法 Download PDF

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Publication number
CN102388145A
CN102388145A CN2009801551526A CN200980155152A CN102388145A CN 102388145 A CN102388145 A CN 102388145A CN 2009801551526 A CN2009801551526 A CN 2009801551526A CN 200980155152 A CN200980155152 A CN 200980155152A CN 102388145 A CN102388145 A CN 102388145A
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cell
cells
antibody
method described
natural killer
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Chinese (zh)
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J·C·洛夫
N·范阮达若詹
B·居尔格
B·沃克
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General Hospital Corp
Massachusetts Institute of Technology
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General Hospital Corp
Massachusetts Institute of Technology
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Priority to CN201510140107.0A priority Critical patent/CN104745669A/zh
Publication of CN102388145A publication Critical patent/CN102388145A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/06—Methods of screening libraries by measuring effects on living organisms, tissues or cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16—HIV-1, HIV-2

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CN2009801551526A 2009-01-21 2009-07-13 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法 Pending CN102388145A (zh)

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CN201510140107.0A CN104745669A (zh) 2009-01-21 2009-07-13 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法

Applications Claiming Priority (3)

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US14610609P 2009-01-21 2009-01-21
US61/146,106 2009-01-21
PCT/US2009/050411 WO2010085275A1 (en) 2009-01-21 2009-07-13 Compositions and methods for assessing cytotoxicity of single cells

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CN201510140107.0A Pending CN104745669A (zh) 2009-01-21 2009-07-13 用于对单个细胞所具有的细胞毒性进行评价的组合物以及方法

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US (1) US9244071B2 (enExample)
EP (1) EP2389446B1 (enExample)
JP (2) JP2012515548A (enExample)
KR (1) KR20110134386A (enExample)
CN (2) CN102388145A (enExample)
AU (1) AU2009338127B2 (enExample)
CA (1) CA2689681C (enExample)
IL (1) IL213938A0 (enExample)
RU (1) RU2532228C2 (enExample)
SG (2) SG172889A1 (enExample)
WO (1) WO2010085275A1 (enExample)

Cited By (5)

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CN105593376A (zh) * 2013-08-26 2016-05-18 哈佛学院院长等 免疫细胞及其它细胞的测定
CN106086171A (zh) * 2016-06-15 2016-11-09 广州中大南沙科技创新产业园有限公司 一种抑制肿瘤细胞增殖的药物筛选方法
CN110998330A (zh) * 2017-06-29 2020-04-10 迈博太科产品公司 用于分析荧光免疫斑点测定的方法和系统
US12345708B2 (en) 2016-03-25 2025-07-01 President And Fellows Of Harvard College Microfluidic determination of immune and other cells
US12391914B2 (en) 2022-11-07 2025-08-19 Shennon Biotechnologies Inc. Microfluidic devices for high throughput screening of cell-cell interactions

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EP2370814B1 (en) 2008-12-04 2015-04-29 Massachusetts Institute of Technology Method for diagnosing allergic reactions
US9404924B2 (en) 2008-12-04 2016-08-02 Massachusetts Institute Of Technology Method of performing one-step, single cell RT-PCR
AU2009338127B2 (en) 2009-01-21 2015-11-26 Massachusetts Institute Of Technology Compositions and methods for assessing cytotoxicity of single cells
EP2646798B1 (en) 2010-12-03 2017-06-28 Cellply S.R.L. Microanalysis of cellular function
EP2646830B1 (en) * 2010-12-03 2016-04-13 Cellply S.R.L. Rapid screening of monoclonal antibodies
CA2856868C (en) * 2011-12-13 2020-07-07 Single Cell Technology, Inc. Method of screening a plurality of single secreting cells for functional activity
US20150191772A1 (en) 2012-06-29 2015-07-09 Danmarks Tekniske Universitet Method of charging a test carrier and a test carrier
CN104884605B (zh) * 2012-08-24 2018-05-18 耶鲁大学 用于高通量多重检测的系统、装置和方法
US9877486B2 (en) 2014-01-31 2018-01-30 AgBiome, Inc. Methods of growing plants using modified biological control agents
US10508280B2 (en) 2014-01-31 2019-12-17 AgBiome, Inc. Modified biological control agents and their uses
EP3227684B1 (en) 2014-12-03 2019-10-02 Isoplexis Corporation Analysis and screening of cell secretion profiles
US10488321B2 (en) 2015-03-19 2019-11-26 The Board Of Trustees Of The Leland Stanford Junior University Devices and methods for high-throughput single cell and biomolecule analysis and retrieval in a microfluidic chip
CN107667178A (zh) 2015-03-26 2018-02-06 休斯敦大学系统 细胞的整合功能和分子概况
WO2017124101A2 (en) 2016-01-15 2017-07-20 The Broad Institute Inc. Semi-permeable arrays for analyzing biological systems and methods of using same
WO2017169267A1 (ja) * 2016-03-30 2017-10-05 ソニー株式会社 細胞観察装置、免疫細胞の活性度の評価方法及び免疫細胞の品質管理方法
SG11201811104TA (en) 2016-06-14 2019-01-30 Cellply S R L Screening kit and method
EP3472351B1 (de) 2016-06-15 2020-10-28 Ludwig-Maximilians-Universität München Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie
EP3510398A1 (en) 2016-09-12 2019-07-17 Isoplexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
EP3532600A4 (en) * 2016-10-19 2020-09-16 General Automation LAB Technologies Inc. HIGH RESOLUTION SYSTEMS, KITS, DEVICES AND METHODS FOR SCREENING MICRO-ORGANISMS AND OTHER HIGH-PERFORMANCE MICROBIOLOGICAL APPLICATIONS
US11493508B2 (en) 2016-11-11 2022-11-08 IsoPlexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
US11085912B2 (en) * 2016-12-14 2021-08-10 University Of Cincinnati Methods of diagnosing Clostridium difficile infection or recurrence in a subject
CA3084439A1 (en) 2017-12-07 2019-06-13 Massachusetts Institute Of Technology Single cell analyses
CN108728356B (zh) * 2018-05-23 2022-02-22 北京科技大学 用于不同三维细胞团配对的装置及共培养方法
CN113614532A (zh) * 2019-01-16 2021-11-05 烟台澳斯邦生物工程有限公司 沉积用于显微镜检查的生物样品的自动液体处理系统和方法
KR102800844B1 (ko) 2019-02-18 2025-04-29 가부시키가이샤 에누비 켄코우겡큐쇼 세포의 선발 방법, 핵산의 제조 방법, 재조합 세포의 제조 방법, 목적 물질의 제조 방법, 의약 조성물의 제조 방법, 및 시약
US12064768B2 (en) 2020-02-21 2024-08-20 Northeastern University Single cell isolation and processing system with reversible well shape

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105593376A (zh) * 2013-08-26 2016-05-18 哈佛学院院长等 免疫细胞及其它细胞的测定
US12345708B2 (en) 2016-03-25 2025-07-01 President And Fellows Of Harvard College Microfluidic determination of immune and other cells
CN106086171A (zh) * 2016-06-15 2016-11-09 广州中大南沙科技创新产业园有限公司 一种抑制肿瘤细胞增殖的药物筛选方法
CN110998330A (zh) * 2017-06-29 2020-04-10 迈博太科产品公司 用于分析荧光免疫斑点测定的方法和系统
CN110998330B (zh) * 2017-06-29 2024-05-10 迈博太科股份公司 用于分析荧光免疫斑点测定的方法和系统
US12092577B2 (en) 2017-06-29 2024-09-17 Mabtech Ab Method and system for analysing FluoroSpot assays
US12391914B2 (en) 2022-11-07 2025-08-19 Shennon Biotechnologies Inc. Microfluidic devices for high throughput screening of cell-cell interactions

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Publication number Publication date
US20120149592A1 (en) 2012-06-14
JP2012515548A (ja) 2012-07-12
AU2009338127A1 (en) 2011-07-28
WO2010085275A1 (en) 2010-07-29
SG10201401643PA (en) 2014-08-28
AU2009338127B2 (en) 2015-11-26
EP2389446A4 (en) 2012-10-03
SG172889A1 (en) 2011-08-29
CA2689681C (en) 2017-08-29
RU2532228C2 (ru) 2014-10-27
RU2011134898A (ru) 2013-02-27
JP2015144617A (ja) 2015-08-13
IL213938A0 (en) 2011-07-31
JP6017620B2 (ja) 2016-11-02
CN104745669A (zh) 2015-07-01
KR20110134386A (ko) 2011-12-14
EP2389446B1 (en) 2017-07-05
EP2389446A1 (en) 2011-11-30
US9244071B2 (en) 2016-01-26
CA2689681A1 (en) 2010-07-21

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Application publication date: 20120321