SG172889A1 - Compositions and methods for assessing cytotoxicity of single cells - Google Patents

Compositions and methods for assessing cytotoxicity of single cells Download PDF

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Publication number
SG172889A1
SG172889A1 SG2011049459A SG2011049459A SG172889A1 SG 172889 A1 SG172889 A1 SG 172889A1 SG 2011049459 A SG2011049459 A SG 2011049459A SG 2011049459 A SG2011049459 A SG 2011049459A SG 172889 A1 SG172889 A1 SG 172889A1
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Singapore
Prior art keywords
cells
cell
microwell
hiv
effector
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SG2011049459A
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English (en)
Inventor
John Christopher Love
Navin Varadarajan
Boris Julg
Bruce Walker
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Massachusetts Inst Technology
Gen Hospital Corp
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Publication date
Application filed by Massachusetts Inst Technology, Gen Hospital Corp filed Critical Massachusetts Inst Technology
Publication of SG172889A1 publication Critical patent/SG172889A1/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966Animal cells
    • G01N33/56972White blood cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047Cells of the immune system
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/563Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00Methods of screening libraries
    • C40B30/06Methods of screening libraries by measuring effects on living organisms, tissues or cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08RNA viruses
    • G01N2333/15Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16HIV-1, HIV-2

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
SG2011049459A 2009-01-21 2009-07-13 Compositions and methods for assessing cytotoxicity of single cells SG172889A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14610609P 2009-01-21 2009-01-21
PCT/US2009/050411 WO2010085275A1 (en) 2009-01-21 2009-07-13 Compositions and methods for assessing cytotoxicity of single cells

Publications (1)

Publication Number Publication Date
SG172889A1 true SG172889A1 (en) 2011-08-29

Family

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Family Applications (2)

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SG10201401643PA SG10201401643PA (en) 2009-01-21 2009-07-13 Methods for assessing cytotoxicity of single cells
SG2011049459A SG172889A1 (en) 2009-01-21 2009-07-13 Compositions and methods for assessing cytotoxicity of single cells

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG10201401643PA SG10201401643PA (en) 2009-01-21 2009-07-13 Methods for assessing cytotoxicity of single cells

Country Status (11)

Country Link
US (1) US9244071B2 (enExample)
EP (1) EP2389446B1 (enExample)
JP (2) JP2012515548A (enExample)
KR (1) KR20110134386A (enExample)
CN (2) CN104745669A (enExample)
AU (1) AU2009338127B2 (enExample)
CA (1) CA2689681C (enExample)
IL (1) IL213938A0 (enExample)
RU (1) RU2532228C2 (enExample)
SG (2) SG10201401643PA (enExample)
WO (1) WO2010085275A1 (enExample)

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CN102308213B (zh) 2008-12-04 2014-10-29 麻省理工学院 诊断过敏性反应的方法
US9404924B2 (en) 2008-12-04 2016-08-02 Massachusetts Institute Of Technology Method of performing one-step, single cell RT-PCR
CA2689681C (en) 2009-01-21 2017-08-29 Massachusetts Institute Of Technology Compositions and methods for assessing cytotoxicity of single cells
WO2012072823A1 (en) * 2010-12-03 2012-06-07 Mindseeds Laboratories Srl Rapid screening of monoclonal antibodies
EP3035031B1 (en) 2010-12-03 2022-06-01 Cellply S.R.L. Microanalysis of cellular function
EP2791358B1 (en) * 2011-12-13 2017-06-14 Single Cell Technology, Inc. Method of screening a plurality of single secreting cells for functional activity
US20150191772A1 (en) 2012-06-29 2015-07-09 Danmarks Tekniske Universitet Method of charging a test carrier and a test carrier
JP6197039B2 (ja) * 2012-08-24 2017-09-13 イェール ユニバーシティーYale University ハイスループット多重検出用システム、装置及び方法
EP3039162B1 (en) * 2013-08-26 2019-01-02 President and Fellows of Harvard College Determination of immune cells and other cells
US9877486B2 (en) 2014-01-31 2018-01-30 AgBiome, Inc. Methods of growing plants using modified biological control agents
HRP20211763T1 (hr) 2014-01-31 2022-02-18 AgBiome, Inc. Modificirano sredstvo za biološku kontrolu i njegove primjene
WO2016090148A1 (en) 2014-12-03 2016-06-09 IsoPlexis Corporation Analysis and screening of cell secretion profiles
US10488321B2 (en) 2015-03-19 2019-11-26 The Board Of Trustees Of The Leland Stanford Junior University Devices and methods for high-throughput single cell and biomolecule analysis and retrieval in a microfluidic chip
EP3274720B1 (en) 2015-03-26 2023-12-27 University of Houston System Integrated functional and molecular profiling of cells
EP3402902B1 (en) 2016-01-15 2021-10-27 Massachusetts Institute Of Technology Semi-permeable arrays for analyzing biological systems and methods of using same
WO2017165791A1 (en) 2016-03-25 2017-09-28 President And Fellows Of Harvard College Microfluidic determination of immune and other cells
US20190106666A1 (en) * 2016-03-30 2019-04-11 Sony Corporation Cell observation device, method for evaluating activity level of immune cells, and method for controlling quality of immune cells
EP3469376B1 (en) 2016-06-14 2023-09-06 Cellply S.R.L. Screening method
CN109563539A (zh) 2016-06-15 2019-04-02 慕尼黑路德维希马克西米利安斯大学 使用dna纳米技术的单分子检测或定量
CN106086171A (zh) * 2016-06-15 2016-11-09 广州中大南沙科技创新产业园有限公司 一种抑制肿瘤细胞增殖的药物筛选方法
WO2018049418A1 (en) 2016-09-12 2018-03-15 IsoPlexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
CN110168069A (zh) * 2016-10-19 2019-08-23 通用自动化实验技术公司 用于筛选微生物和其他高通量微生物学应用的高分辨率系统、试剂盒、设备和方法
EP4036578A1 (en) * 2016-11-11 2022-08-03 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
US11085912B2 (en) * 2016-12-14 2021-08-10 University Of Cincinnati Methods of diagnosing Clostridium difficile infection or recurrence in a subject
SE543211C2 (en) 2017-06-29 2020-10-27 Mabtech Production Ab Method and system for analyzing Fluorospot assays
AU2018378827B2 (en) 2017-12-07 2023-04-13 Massachusetts Institute Of Technology Single cell analyses
CN108728356B (zh) * 2018-05-23 2022-02-22 北京科技大学 用于不同三维细胞团配对的装置及共培养方法
CN113614532A (zh) * 2019-01-16 2021-11-05 烟台澳斯邦生物工程有限公司 沉积用于显微镜检查的生物样品的自动液体处理系统和方法
WO2020171020A1 (ja) * 2019-02-18 2020-08-27 株式会社エヌビィー健康研究所 細胞の選抜方法、核酸の製造方法、組換え細胞の製造方法、目的物質の製造方法、医薬組成物の製造方法、及び試薬
US12064768B2 (en) 2020-02-21 2024-08-20 Northeastern University Single cell isolation and processing system with reversible well shape
CN120693393A (zh) 2022-11-07 2025-09-23 神农生物技术公司 用于高通量筛选细胞间相互作用的微流体装置

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Also Published As

Publication number Publication date
EP2389446B1 (en) 2017-07-05
WO2010085275A1 (en) 2010-07-29
CA2689681C (en) 2017-08-29
US9244071B2 (en) 2016-01-26
RU2011134898A (ru) 2013-02-27
RU2532228C2 (ru) 2014-10-27
CN104745669A (zh) 2015-07-01
EP2389446A4 (en) 2012-10-03
JP2012515548A (ja) 2012-07-12
AU2009338127A1 (en) 2011-07-28
CA2689681A1 (en) 2010-07-21
AU2009338127B2 (en) 2015-11-26
EP2389446A1 (en) 2011-11-30
JP6017620B2 (ja) 2016-11-02
JP2015144617A (ja) 2015-08-13
CN102388145A (zh) 2012-03-21
IL213938A0 (en) 2011-07-31
KR20110134386A (ko) 2011-12-14
SG10201401643PA (en) 2014-08-28
US20120149592A1 (en) 2012-06-14

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