JP2015144617A5 - - Google Patents
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- JP2015144617A5 JP2015144617A5 JP2015094509A JP2015094509A JP2015144617A5 JP 2015144617 A5 JP2015144617 A5 JP 2015144617A5 JP 2015094509 A JP2015094509 A JP 2015094509A JP 2015094509 A JP2015094509 A JP 2015094509A JP 2015144617 A5 JP2015144617 A5 JP 2015144617A5
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- Prior art keywords
- cells
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- microwell
- substrate
- target cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims description 106
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- 239000000758 substrate Substances 0.000 claims description 41
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- 108090000623 proteins and genes Proteins 0.000 claims description 14
- 230000009089 cytolysis Effects 0.000 claims description 13
- 238000012258 culturing Methods 0.000 claims description 12
- 102000004127 Cytokines Human genes 0.000 claims description 11
- 108090000695 Cytokines Proteins 0.000 claims description 11
- 239000003550 marker Substances 0.000 claims description 10
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 claims description 8
- 238000012544 monitoring process Methods 0.000 claims description 8
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- 230000004913 activation Effects 0.000 claims 6
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- 239000003269 fluorescent indicator Substances 0.000 claims 1
- 210000005260 human cell Anatomy 0.000 claims 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 59
- 210000000822 natural killer cell Anatomy 0.000 description 45
- 241000725303 Human immunodeficiency virus Species 0.000 description 35
- 210000002845 virion Anatomy 0.000 description 18
- 239000000725 suspension Substances 0.000 description 15
- 238000001514 detection method Methods 0.000 description 12
- 108010029697 CD40 Ligand Proteins 0.000 description 11
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- 210000001744 T-lymphocyte Anatomy 0.000 description 10
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- 238000000151 deposition Methods 0.000 description 8
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- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical group OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 6
- 102100037850 Interferon gamma Human genes 0.000 description 6
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- 108010002350 Interleukin-2 Proteins 0.000 description 6
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- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 6
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- 210000000581 natural killer T-cell Anatomy 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
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- 102100032976 CCR4-NOT transcription complex subunit 6 Human genes 0.000 description 2
- 102100025137 Early activation antigen CD69 Human genes 0.000 description 2
- 208000031886 HIV Infections Diseases 0.000 description 2
- 208000037357 HIV infectious disease Diseases 0.000 description 2
- 101000716065 Homo sapiens C-C chemokine receptor type 7 Proteins 0.000 description 2
- 101000916050 Homo sapiens C-X-C chemokine receptor type 3 Proteins 0.000 description 2
- 101000934374 Homo sapiens Early activation antigen CD69 Proteins 0.000 description 2
- 101001018097 Homo sapiens L-selectin Proteins 0.000 description 2
- 101000917858 Homo sapiens Low affinity immunoglobulin gamma Fc region receptor III-A Proteins 0.000 description 2
- 101000917839 Homo sapiens Low affinity immunoglobulin gamma Fc region receptor III-B Proteins 0.000 description 2
- 108090000174 Interleukin-10 Proteins 0.000 description 2
- 108090000978 Interleukin-4 Proteins 0.000 description 2
- 102000002698 KIR Receptors Human genes 0.000 description 2
- 108010043610 KIR Receptors Proteins 0.000 description 2
- 102100033467 L-selectin Human genes 0.000 description 2
- 102100029185 Low affinity immunoglobulin gamma Fc region receptor III-B Human genes 0.000 description 2
- KHGNFPUMBJSZSM-UHFFFAOYSA-N Perforine Natural products COC1=C2CCC(O)C(CCC(C)(C)O)(OC)C2=NC2=C1C=CO2 KHGNFPUMBJSZSM-UHFFFAOYSA-N 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 238000011225 antiretroviral therapy Methods 0.000 description 2
- BQRGNLJZBFXNCZ-UHFFFAOYSA-N calcein am Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)=C(OC(C)=O)C=C1OC1=C2C=C(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(=O)C)C(OC(C)=O)=C1 BQRGNLJZBFXNCZ-UHFFFAOYSA-N 0.000 description 2
- 230000009260 cross reactivity Effects 0.000 description 2
- 208000033519 human immunodeficiency virus infectious disease Diseases 0.000 description 2
- 230000002934 lysing effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
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- 230000003472 neutralizing effect Effects 0.000 description 2
- 229930192851 perforin Natural products 0.000 description 2
- 238000003757 reverse transcription PCR Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 206010069754 Acquired gene mutation Diseases 0.000 description 1
- 108010067060 Immunoglobulin Variable Region Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- DZNKOAWEHDKBEP-UHFFFAOYSA-N methyl 2-[6-[bis(2-methoxy-2-oxoethyl)amino]-5-[2-[2-[bis(2-methoxy-2-oxoethyl)amino]-5-methylphenoxy]ethoxy]-1-benzofuran-2-yl]-1,3-oxazole-5-carboxylate Chemical group COC(=O)CN(CC(=O)OC)C1=CC=C(C)C=C1OCCOC(C(=C1)N(CC(=O)OC)CC(=O)OC)=CC2=C1OC(C=1OC(=CN=1)C(=O)OC)=C2 DZNKOAWEHDKBEP-UHFFFAOYSA-N 0.000 description 1
- 108010043277 recombinant soluble CD4 Proteins 0.000 description 1
- 238000007423 screening assay Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000037439 somatic mutation Effects 0.000 description 1
- 238000012289 standard assay Methods 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14610609P | 2009-01-21 | 2009-01-21 | |
| US61/146,106 | 2009-01-21 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011547913A Division JP2012515548A (ja) | 2009-01-21 | 2009-07-13 | 単一細胞の細胞傷害性を査定するための組成物および方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015144617A JP2015144617A (ja) | 2015-08-13 |
| JP2015144617A5 true JP2015144617A5 (enExample) | 2016-06-23 |
| JP6017620B2 JP6017620B2 (ja) | 2016-11-02 |
Family
ID=42356144
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011547913A Withdrawn JP2012515548A (ja) | 2009-01-21 | 2009-07-13 | 単一細胞の細胞傷害性を査定するための組成物および方法 |
| JP2015094509A Active JP6017620B2 (ja) | 2009-01-21 | 2015-05-05 | 単一細胞の細胞傷害性を査定するための組成物および方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011547913A Withdrawn JP2012515548A (ja) | 2009-01-21 | 2009-07-13 | 単一細胞の細胞傷害性を査定するための組成物および方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US9244071B2 (enExample) |
| EP (1) | EP2389446B1 (enExample) |
| JP (2) | JP2012515548A (enExample) |
| KR (1) | KR20110134386A (enExample) |
| CN (2) | CN104745669A (enExample) |
| AU (1) | AU2009338127B2 (enExample) |
| CA (1) | CA2689681C (enExample) |
| IL (1) | IL213938A0 (enExample) |
| RU (1) | RU2532228C2 (enExample) |
| SG (2) | SG10201401643PA (enExample) |
| WO (1) | WO2010085275A1 (enExample) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9404924B2 (en) | 2008-12-04 | 2016-08-02 | Massachusetts Institute Of Technology | Method of performing one-step, single cell RT-PCR |
| EP2370814B1 (en) | 2008-12-04 | 2015-04-29 | Massachusetts Institute of Technology | Method for diagnosing allergic reactions |
| SG10201401643PA (en) | 2009-01-21 | 2014-08-28 | Massachusetts Inst Technology | Methods for assessing cytotoxicity of single cells |
| CN103392124B (zh) | 2010-12-03 | 2016-04-20 | 塞普莱有限公司 | 细胞功能的微分析 |
| US10571475B2 (en) * | 2010-12-03 | 2020-02-25 | Cellply S.R.L. | Rapid screening of monoclonal antibodies |
| US9944983B2 (en) | 2011-12-13 | 2018-04-17 | Single Cell Technology, Inc. | Method of screening a plurality of single secreting cells for functional activity |
| WO2014001459A1 (en) | 2012-06-29 | 2014-01-03 | Danmarks Tekniske Universitet | A method of charging a test carrier and a test carrier |
| JP6197039B2 (ja) * | 2012-08-24 | 2017-09-13 | イェール ユニバーシティーＹａｌｅ Ｕｎｉｖｅｒｓｉｔｙ | ハイスループット多重検出用システム、装置及び方法 |
| EP3039162B1 (en) * | 2013-08-26 | 2019-01-02 | President and Fellows of Harvard College | Determination of immune cells and other cells |
| BR122022010122B1 (pt) | 2014-01-31 | 2023-05-16 | Agbiome, Inc | Composição, uso da composição, método para controlar um patógeno vegetal, formulação para o controle de um patógeno vegetal, uso da formulação, método para cultivar uma planta e uso de um agente de controle biológico nrrl n° b-50897 |
| US9877486B2 (en) | 2014-01-31 | 2018-01-30 | AgBiome, Inc. | Methods of growing plants using modified biological control agents |
| WO2016090148A1 (en) | 2014-12-03 | 2016-06-09 | IsoPlexis Corporation | Analysis and screening of cell secretion profiles |
| WO2016149639A1 (en) | 2015-03-19 | 2016-09-22 | The Board Of Trustees Of The Leland Stanford Junior University | Devices and methods for high-throughput single cell and biomolecule analysis and retrieval in a microfluidic chip |
| CN107667178A (zh) | 2015-03-26 | 2018-02-06 | 休斯敦大学系统 | 细胞的整合功能和分子概况 |
| EP3402902B1 (en) | 2016-01-15 | 2021-10-27 | Massachusetts Institute Of Technology | Semi-permeable arrays for analyzing biological systems and methods of using same |
| US12345708B2 (en) | 2016-03-25 | 2025-07-01 | President And Fellows Of Harvard College | Microfluidic determination of immune and other cells |
| WO2017169267A1 (ja) * | 2016-03-30 | 2017-10-05 | ソニー株式会社 | 細胞観察装置、免疫細胞の活性度の評価方法及び免疫細胞の品質管理方法 |
| RU2739327C2 (ru) | 2016-06-14 | 2020-12-23 | Селплай С.Р.Л. | Набор и способ скрининга |
| US11513076B2 (en) | 2016-06-15 | 2022-11-29 | Ludwig-Maximilians-Universität München | Single molecule detection or quantification using DNA nanotechnology |
| CN106086171A (zh) * | 2016-06-15 | 2016-11-09 | 广州中大南沙科技创新产业园有限公司 | 一种抑制肿瘤细胞增殖的药物筛选方法 |
| WO2018049418A1 (en) | 2016-09-12 | 2018-03-15 | IsoPlexis Corporation | System and methods for multiplexed analysis of cellular and other immunotherapeutics |
| CA3041109A1 (en) * | 2016-10-19 | 2018-04-26 | General Automation Lab Technologies Inc. | High resolution systems, kits, apparatus, and methods for screening microorganisms and other high throughput microbiology applications |
| EP4036578A1 (en) * | 2016-11-11 | 2022-08-03 | Isoplexis Corporation | Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells |
| US11085912B2 (en) * | 2016-12-14 | 2021-08-10 | University Of Cincinnati | Methods of diagnosing Clostridium difficile infection or recurrence in a subject |
| SE543211C2 (en) | 2017-06-29 | 2020-10-27 | Mabtech Production Ab | Method and system for analyzing Fluorospot assays |
| JP2021505157A (ja) | 2017-12-07 | 2021-02-18 | マサチューセッツ インスティテュート オブ テクノロジー | 単一細胞分析 |
| CN108728356B (zh) * | 2018-05-23 | 2022-02-22 | 北京科技大学 | 用于不同三维细胞团配对的装置及共培养方法 |
| CN113614532A (zh) * | 2019-01-16 | 2021-11-05 | 烟台澳斯邦生物工程有限公司 | 沉积用于显微镜检查的生物样品的自动液体处理系统和方法 |
| CA3130051A1 (en) * | 2019-02-18 | 2020-08-27 | Nb Health Laboratory Co., Ltd. | Method for selecting cells, method for producing nucleic acid, method for producing recombinant cells, method for producing target substance, method for producing pharmaceutical composition, and reagent |
| US12064768B2 (en) | 2020-02-21 | 2024-08-20 | Northeastern University | Single cell isolation and processing system with reversible well shape |
| JP2025535563A (ja) | 2022-11-07 | 2025-10-24 | シェンノン バイオテクノロジーズ インコーポレイテッド | 細胞－細胞相互作用のハイスループットスクリーニングのためのマイクロ流体デバイス |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL68507A (en) | 1982-05-10 | 1986-01-31 | Univ Bar Ilan | System and methods for cell selection |
| SU1650094A1 (ru) * | 1987-12-29 | 1991-05-23 | Научно-исследовательский институт проктологии | Способ диагностики прот женности активного воспалени в толстой кишке при неспецифическом звенном колите |
| US6410252B1 (en) | 1995-12-22 | 2002-06-25 | Case Western Reserve University | Methods for measuring T cell cytokines |
| US6210910B1 (en) | 1998-03-02 | 2001-04-03 | Trustees Of Tufts College | Optical fiber biosensor array comprising cell populations confined to microcavities |
| CA2370489A1 (en) * | 1999-04-09 | 2000-10-19 | Human Genome Sciences, Inc. | 49 human secreted proteins |
| JP2003502062A (ja) * | 1999-06-11 | 2003-01-21 | ヒューマン ジノーム サイエンシーズ， インコーポレイテッド | ４９個のヒト分泌タンパク質 |
| US7332286B2 (en) * | 2001-02-02 | 2008-02-19 | University Of Pennsylvania | Peptide or protein microassay method and apparatus |
| US20020141906A1 (en) | 2001-03-30 | 2002-10-03 | American Type Culture Collection | Microtiter plate sealing cover with well identifiers |
| US7776553B2 (en) | 2005-09-16 | 2010-08-17 | Presidents And Fellows Of Harvard College | Screening assays and methods |
| DK2297333T3 (en) | 2008-05-30 | 2015-04-07 | Massachusetts Inst Technology | Method for spatial separation and for screening cells |
| US9404924B2 (en) | 2008-12-04 | 2016-08-02 | Massachusetts Institute Of Technology | Method of performing one-step, single cell RT-PCR |
| EP2370814B1 (en) | 2008-12-04 | 2015-04-29 | Massachusetts Institute of Technology | Method for diagnosing allergic reactions |
| SG10201401643PA (en) | 2009-01-21 | 2014-08-28 | Massachusetts Inst Technology | Methods for assessing cytotoxicity of single cells |
| US8569046B2 (en) | 2009-02-20 | 2013-10-29 | Massachusetts Institute Of Technology | Microarray with microchannels |
-
2009
- 2009-07-13 SG SG10201401643PA patent/SG10201401643PA/en unknown
- 2009-07-13 EP EP09839012.3A patent/EP2389446B1/en not_active Not-in-force
- 2009-07-13 WO PCT/US2009/050411 patent/WO2010085275A1/en not_active Ceased
- 2009-07-13 CN CN201510140107.0A patent/CN104745669A/zh active Pending
- 2009-07-13 CN CN2009801551526A patent/CN102388145A/zh active Pending
- 2009-07-13 SG SG2011049459A patent/SG172889A1/en unknown
- 2009-07-13 JP JP2011547913A patent/JP2012515548A/ja not_active Withdrawn
- 2009-07-13 US US13/145,300 patent/US9244071B2/en active Active
- 2009-07-13 RU RU2011134898/10A patent/RU2532228C2/ru not_active IP Right Cessation
- 2009-07-13 CA CA2689681A patent/CA2689681C/en not_active Expired - Fee Related
- 2009-07-13 KR KR1020117018895A patent/KR20110134386A/ko not_active Ceased
- 2009-07-13 AU AU2009338127A patent/AU2009338127B2/en not_active Ceased
-
2011
- 2011-07-05 IL IL213938A patent/IL213938A0/en unknown
-
2015
- 2015-05-05 JP JP2015094509A patent/JP6017620B2/ja active Active
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