RU2509152C2 - Cпособ мытья посуды - Google Patents
Cпособ мытья посуды Download PDFInfo
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- RU2509152C2 RU2509152C2 RU2009105503/10A RU2009105503A RU2509152C2 RU 2509152 C2 RU2509152 C2 RU 2509152C2 RU 2009105503/10 A RU2009105503/10 A RU 2009105503/10A RU 2009105503 A RU2009105503 A RU 2009105503A RU 2509152 C2 RU2509152 C2 RU 2509152C2
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- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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| AU2008226792B2 (en) | 2007-03-12 | 2013-06-06 | Danisco Us Inc. | Modified proteases |
| EP2100948A1 (en) * | 2008-03-14 | 2009-09-16 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| EP2100947A1 (en) * | 2008-03-14 | 2009-09-16 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| MX383345B (es) * | 2008-06-06 | 2025-03-13 | Danisco Us Inc | Composiciones y metodos que comprenden proteasas microbianas variantes. |
| MX383346B (es) * | 2008-11-11 | 2025-03-13 | Danisco Us Inc | Composiciones y metodos que comprenden variantes de serina proteasa. |
| CN106399280B (zh) | 2008-11-11 | 2020-12-22 | 丹尼斯科美国公司 | 包含一种或多种可组合的突变的芽孢杆菌属枯草杆菌蛋白酶 |
| WO2010056671A1 (en) * | 2008-11-11 | 2010-05-20 | Danisco Us Inc. | Compositions and methods comprising a subtilisin variant |
| BRPI0920925A2 (pt) | 2008-11-11 | 2015-08-18 | Danisco Us Inc | Composições e métodos compreendendo uma variante de subtilisina |
| EP2216393B1 (en) * | 2009-02-09 | 2024-04-24 | The Procter & Gamble Company | Detergent composition |
| BRPI1013388A2 (pt) | 2009-04-01 | 2019-04-09 | Danisco Us Inc | composição de limpeza que compreende uma alfa-amilase e uma protease e método de limpeza de um tecido ou superficie dura |
| JP5947213B2 (ja) * | 2009-09-25 | 2016-07-06 | ノボザイムス アクティーゼルスカブ | プロテアーゼ変異体の使用 |
| BR112012006497A2 (pt) * | 2009-09-25 | 2015-09-08 | Novozymes As | uso de uma variante de subtilisina, composição de lavagem de pratos, e, uso de uma composição. |
| CA2782891C (en) | 2009-12-09 | 2022-01-11 | Danisco Us Inc. | Compositions and methods comprising protease variants |
| EP2333039B2 (en) | 2009-12-10 | 2020-11-11 | The Procter & Gamble Company | Method and use of a dishwasher composition |
| EP2333041B1 (en) | 2009-12-10 | 2013-05-15 | The Procter & Gamble Company | Method and use of a dishwasher composition |
| ES2399311T5 (es) | 2009-12-10 | 2020-06-19 | Procter & Gamble | Composición detergente |
| EP2333042B1 (en) | 2009-12-10 | 2015-07-01 | The Procter and Gamble Company | Automatic dishwashing product and use thereof |
| CN102712879A (zh) | 2009-12-21 | 2012-10-03 | 丹尼斯科美国公司 | 含有褐色喜热裂孢菌脂肪酶的洗涤剂组合物及其使用方法 |
| US8741609B2 (en) | 2009-12-21 | 2014-06-03 | Danisco Us Inc. | Detergent compositions containing Geobacillus stearothermophilus lipase and methods of use thereof |
| CN102712878A (zh) | 2009-12-21 | 2012-10-03 | 丹尼斯科美国公司 | 含有枯草芽孢杆菌脂肪酶的洗涤剂组合物及其使用方法 |
| ES2401126T3 (es) | 2010-02-25 | 2013-04-17 | The Procter & Gamble Company | Composición detergente |
| WO2011130222A2 (en) | 2010-04-15 | 2011-10-20 | Danisco Us Inc. | Compositions and methods comprising variant proteases |
| US20120067373A1 (en) * | 2010-04-15 | 2012-03-22 | Philip Frank Souter | Automatic Dishwashing Detergent Composition |
| EP2380963B1 (en) | 2010-04-23 | 2015-12-23 | The Procter and Gamble Company | Method of perfuming |
| EP2383329A1 (en) | 2010-04-23 | 2011-11-02 | The Procter & Gamble Company | Particle |
| PL2380961T3 (pl) | 2010-04-23 | 2018-10-31 | The Procter & Gamble Company | Kompozycja detergentu |
| EP2380962B1 (en) | 2010-04-23 | 2016-03-30 | The Procter and Gamble Company | Particle |
| ES2533368T3 (es) | 2010-04-23 | 2015-04-09 | The Procter & Gamble Company | Producto para lavavajillas |
| EP2380478A1 (en) | 2010-04-23 | 2011-10-26 | The Procter & Gamble Company | Automatic dishwashing product |
| EP3868881B1 (en) | 2010-05-06 | 2025-07-23 | Danisco US Inc. | Compositions and methods comprising subtilisin variants |
| WO2011150157A2 (en) | 2010-05-28 | 2011-12-01 | Danisco Us Inc. | Detergent compositions containing streptomyces griseus lipase and methods of use thereof |
| DE102011007313A1 (de) * | 2011-04-13 | 2012-10-18 | Henkel Ag & Co. Kgaa | Expressionsverfahren |
| AR086216A1 (es) | 2011-04-29 | 2013-11-27 | Danisco Us Inc | Composiciones detergentes que contienen mananasa de bacillus sp. y sus metodos de uso |
| WO2012149317A1 (en) | 2011-04-29 | 2012-11-01 | Danisco Us Inc. | Detergent compositions containing bacillus agaradhaerens mannanase and methods of use thereof |
| AR086215A1 (es) | 2011-04-29 | 2013-11-27 | Danisco Us Inc | Composiciones detergentes que contienen mananasa de geobacillus tepidamans y metodos de uso de las mismas |
| WO2012151480A2 (en) | 2011-05-05 | 2012-11-08 | The Procter & Gamble Company | Compositions and methods comprising serine protease variants |
| CA3111256C (en) | 2011-05-05 | 2023-05-09 | Danisco Us Inc. | Compositions and methods comprising serine protease variants |
| EP3798303A1 (en) | 2011-06-30 | 2021-03-31 | Novozymes A/S | Alpha-amylase-variants |
| EP2584028B1 (en) | 2011-10-19 | 2017-05-10 | The Procter & Gamble Company | Particle |
| EP2794866A1 (en) | 2011-12-22 | 2014-10-29 | Danisco US Inc. | Compositions and methods comprising a lipolytic enzyme variant |
| ES2646416T3 (es) | 2012-05-11 | 2017-12-13 | The Procter & Gamble Company | Composición detergente |
| ES2678543T3 (es) | 2012-08-24 | 2018-08-13 | The Procter & Gamble Company | Método de lavado de vajillas |
| EP2700703B1 (en) | 2012-08-24 | 2018-05-02 | The Procter and Gamble Company | Dishwashing method |
| EP2906691B1 (en) | 2012-10-12 | 2021-01-27 | Danisco US Inc. | Compositions and methods comprising a lipolytic enzyme variant |
| JP6858487B2 (ja) | 2012-11-05 | 2021-04-14 | ダニスコ・ユーエス・インク | サーモリシンプロテアーゼ変異体を含む組成物及び方法 |
| EP2935573A1 (en) | 2012-12-19 | 2015-10-28 | Danisco US Inc. | Novel mannanase, compositions and methods of use thereof |
| WO2014099525A1 (en) | 2012-12-21 | 2014-06-26 | Danisco Us Inc. | Paenibacillus curdlanolyticus amylase, and methods of use, thereof |
| DK2935575T3 (en) | 2012-12-21 | 2018-07-23 | Danisco Us Inc | ALPHA-amylase variants |
| EP2746381A1 (en) | 2012-12-21 | 2014-06-25 | The Procter & Gamble Company | Cleaning pack |
| US20160017305A1 (en) | 2013-03-11 | 2016-01-21 | Danisco Us Inc. | Alpha-amylase combinatorial variants |
| EP3260538B1 (en) | 2013-05-29 | 2021-04-14 | Danisco US Inc. | Novel metalloproteases |
| US20160122738A1 (en) | 2013-05-29 | 2016-05-05 | Danisco Us Inc. | Novel metalloproteases |
| US20160160202A1 (en) | 2013-05-29 | 2016-06-09 | Danisco Us Inc. | Novel metalloproteases |
| US20160108387A1 (en) | 2013-05-29 | 2016-04-21 | Danisco Us Inc. | Novel metalloproteases |
| EP3022299B1 (en) | 2013-07-19 | 2020-03-18 | Danisco US Inc. | Compositions and methods comprising a lipolytic enzyme variant |
| EP3653707B1 (en) | 2013-09-12 | 2025-07-16 | Danisco US Inc. | Compositions and methods comprising lg12-clade protease variants |
| AR098006A1 (es) | 2013-10-15 | 2016-04-27 | Danisco Us Inc | Gránulo de arcilla |
| DK3068879T3 (da) | 2013-11-14 | 2020-03-16 | Danisco Us Inc | Stabile enzymer opnået ved glykeringsreduktion |
| DK3080262T3 (da) | 2013-12-13 | 2019-05-06 | Danisco Us Inc | Serinproteaser af bacillus-arter |
| DK3553173T3 (da) | 2013-12-13 | 2021-08-23 | Danisco Us Inc | Serinproteaser af bacillus gibsonii-clade |
| EP4163305B1 (en) | 2013-12-16 | 2024-07-10 | Nutrition & Biosciences USA 4, Inc. | Use of poly alpha-1,3-glucan ethers as viscosity modifiers |
| CN106029700B (zh) | 2013-12-18 | 2019-04-12 | 纳幕尔杜邦公司 | 阳离子聚α-1，3-葡聚糖醚 |
| WO2015123323A1 (en) | 2014-02-14 | 2015-08-20 | E. I. Du Pont De Nemours And Company | Poly-alpha-1,3-1,6-glucans for viscosity modification |
| ES2734726T3 (es) | 2014-03-11 | 2019-12-11 | Du Pont | Poli alfa-1,2-glucano oxidado como reforzante de detergente |
| EP3587569B1 (en) | 2014-03-21 | 2022-08-03 | Danisco US Inc. | Serine proteases of bacillus species |
| US9714403B2 (en) | 2014-06-19 | 2017-07-25 | E I Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
| WO2015195777A1 (en) | 2014-06-19 | 2015-12-23 | E. I. Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
| US20170306360A1 (en) | 2014-10-24 | 2017-10-26 | Danisco Us Inc. | Method for producing alcohol by use of a tripeptidyl peptidase |
| EP3957729A1 (en) | 2014-10-27 | 2022-02-23 | Danisco US Inc. | Serine proteases |
| DK3212662T3 (da) | 2014-10-27 | 2020-07-20 | Danisco Us Inc | Serinproteaser |
| EP3224357A1 (en) | 2014-10-27 | 2017-10-04 | Danisco US Inc. | Serine proteases of bacillus species |
| EP4403631A3 (en) | 2014-10-27 | 2024-10-30 | Danisco US Inc. | Serine proteases |
| EP3212781B1 (en) | 2014-10-27 | 2019-09-18 | Danisco US Inc. | Serine proteases |
| PL3026099T3 (pl) | 2014-11-26 | 2021-06-14 | The Procter & Gamble Company | Saszetka czyszcząca |
| EP3026103B1 (en) | 2014-11-26 | 2018-07-25 | The Procter and Gamble Company | Cleaning pouch |
| EP3026100B1 (en) | 2014-11-26 | 2018-07-25 | The Procter and Gamble Company | Cleaning pouch |
| ES2714084T3 (es) | 2014-11-26 | 2019-05-27 | Procter & Gamble | Bolsa limpiadora |
| PL3037512T3 (pl) | 2014-12-22 | 2018-08-31 | The Procter And Gamble Company | Proces recyklingu saszetek z detergentem |
| CA2969242A1 (en) | 2014-12-23 | 2016-06-30 | E.I. Du Pont De Nemours And Company | Enzymatically produced cellulose |
| EP3050948B1 (en) | 2015-02-02 | 2018-09-19 | The Procter and Gamble Company | New use of complexing agent |
| EP3050952A1 (en) | 2015-02-02 | 2016-08-03 | The Procter and Gamble Company | Method of dishwashing |
| EP3050950B1 (en) | 2015-02-02 | 2018-09-19 | The Procter and Gamble Company | New use of sulfonated polymers |
| EP3050955B2 (en) | 2015-02-02 | 2023-11-08 | The Procter & Gamble Company | Detergent pack |
| EP3050947A1 (en) | 2015-02-02 | 2016-08-03 | The Procter and Gamble Company | Detergent pack |
| ES2714130T3 (es) | 2015-02-02 | 2019-05-27 | Procter & Gamble | Composición detergente |
| EP3050951A1 (en) | 2015-02-02 | 2016-08-03 | The Procter and Gamble Company | Method of dishwashing |
| EP3050954A1 (en) | 2015-02-02 | 2016-08-03 | The Procter and Gamble Company | New use of sulfonated polymers |
| DK3268471T3 (da) | 2015-03-12 | 2019-12-02 | Danisco Us Inc | Sammensætninger og fremgangsmåder omfattende lg12-clade-proteasevarianter |
| US20180112204A1 (en) | 2015-05-13 | 2018-04-26 | Danisco Us Inc. | AprL-CLADE PROTEASE VARIANTS AND USES THEREOF |
| EP3307427B1 (en) | 2015-06-09 | 2023-08-16 | Danisco US Inc. | Osmotic burst encapsulates |
| WO2016201040A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc. | Water-triggered enzyme suspension |
| WO2016201069A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc | Low-density enzyme-containing particles |
| JP7015695B2 (ja) | 2015-06-17 | 2022-02-03 | ダニスコ・ユーエス・インク | バチルス・ギブソニイ（Ｂａｃｉｌｌｕｓ ｇｉｂｓｏｎｉｉ）クレードセリンプロテアーゼ |
| ES2728108T3 (es) | 2015-07-29 | 2019-10-22 | Procter & Gamble | Producto de limpieza multifásico en dosis unitaria |
| EP3124586A1 (en) | 2015-07-29 | 2017-02-01 | The Procter and Gamble Company | Process for reducing malodour in a pack |
| US20190153417A1 (en) | 2015-11-05 | 2019-05-23 | Danisco Us Inc | Paenibacillus sp. mannanases |
| CN108603183B (zh) | 2015-11-05 | 2023-11-03 | 丹尼斯科美国公司 | 类芽孢杆菌属物种和芽孢杆菌属物种甘露聚糖酶 |
| WO2017083229A1 (en) | 2015-11-13 | 2017-05-18 | E. I. Du Pont De Nemours And Company | Glucan fiber compositions for use in laundry care and fabric care |
| EP3374400B1 (en) | 2015-11-13 | 2022-04-13 | Nutrition & Biosciences USA 4, Inc. | Glucan fiber compositions for use in laundry care and fabric care |
| WO2017083226A1 (en) | 2015-11-13 | 2017-05-18 | E. I. Du Pont De Nemours And Company | Glucan fiber compositions for use in laundry care and fabric care |
| EP3178917A1 (en) | 2015-12-08 | 2017-06-14 | The Procter and Gamble Company | Cleaning pouch |
| EP3387124B1 (en) | 2015-12-09 | 2021-05-19 | Danisco US Inc. | Alpha-amylase combinatorial variants |
| EP3390625B1 (en) | 2015-12-18 | 2023-09-06 | Danisco US Inc. | Polypeptides with endoglucanase activity and uses thereof |
| FI3419991T3 (fi) | 2016-03-04 | 2023-01-31 | Modifioidut ribosomaaliset promoottorit proteiinien tuottamiseksi mikro-organismeissa | |
| EP3228690B1 (en) | 2016-04-08 | 2020-05-13 | The Procter and Gamble Company | Automatic dishwashing cleaning composition |
| JP2019513860A (ja) | 2016-04-08 | 2019-05-30 | ザ プロクター アンド ギャンブル カンパニー | 自動食器洗浄洗剤組成物 |
| EP3228686B1 (en) | 2016-04-08 | 2021-10-27 | The Procter & Gamble Company | Automatic dishwashing |
| EP3228687B1 (en) | 2016-04-08 | 2019-05-22 | The Procter and Gamble Company | Dishwashing cleaning composition |
| EP3241890B1 (en) | 2016-05-03 | 2019-06-26 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| WO2017192653A1 (en) | 2016-05-03 | 2017-11-09 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| BR112018072530A2 (pt) | 2016-05-03 | 2019-03-26 | Danisco Us Inc | variantes de protease e usos das mesmas |
| EP3241891B1 (en) | 2016-05-03 | 2019-04-03 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| EP3845642B1 (en) | 2016-05-05 | 2023-08-09 | Danisco US Inc. | Protease variants and uses thereof |
| CA3026064A1 (en) | 2016-05-31 | 2017-12-07 | Danisco Us Inc. | Protease variants and uses thereof |
| MX2018015559A (es) | 2016-06-17 | 2019-06-06 | Danisco Us Inc | Variantes de proteasa y sus usos. |
| EP3266860B1 (en) | 2016-07-08 | 2020-04-08 | The Procter and Gamble Company | Process for making a particle |
| EP3290503A3 (en) | 2016-09-01 | 2018-05-30 | The Procter & Gamble Company | Automatic dishwashing cleaning composition |
| EP3312265A1 (en) | 2016-10-18 | 2018-04-25 | The Procter and Gamble Company | Detergent composition |
| US20190264138A1 (en) | 2016-11-07 | 2019-08-29 | Danisco Us Inc. | Laundry detergent composition |
| EP3559226B1 (en) | 2016-12-21 | 2023-01-04 | Danisco US Inc. | Bacillus gibsonii-clade serine proteases |
| US20200392477A1 (en) * | 2016-12-21 | 2020-12-17 | Danisco Us Inc. | Protease variants and uses thereof |
| EP3339410A1 (en) | 2016-12-22 | 2018-06-27 | The Procter & Gamble Company | Automatic dishwashing composition |
| EP3583210B1 (en) | 2017-03-15 | 2021-07-07 | Danisco US Inc. | Trypsin-like serine proteases and uses thereof |
| EP3601515A1 (en) | 2017-03-31 | 2020-02-05 | Danisco US Inc. | Delayed release enzyme formulations for bleach-containing detergents |
| CN110662836B (zh) | 2017-03-31 | 2024-04-12 | 丹尼斯科美国公司 | α-淀粉酶组合变体 |
| EP3418364A1 (en) | 2017-06-19 | 2018-12-26 | The Procter & Gamble Company | Automatic dishwashing cleaning composition |
| EP3418365A1 (en) | 2017-06-19 | 2018-12-26 | The Procter & Gamble Company | Automatic dishwashing cleaning composition |
| EP3418366A1 (en) | 2017-06-19 | 2018-12-26 | The Procter & Gamble Company | Automatic dishwashing cleaning composition |
| BR112019027976A2 (pt) | 2017-06-30 | 2020-07-07 | Danisco Us Inc. | partículas de baixa aglomeração, contendo enzimas |
| EP3441450A1 (en) | 2017-08-11 | 2019-02-13 | The Procter & Gamble Company | Automatic dishwashing composition |
| CN111212906B (zh) | 2017-08-18 | 2024-02-02 | 丹尼斯科美国公司 | α-淀粉酶变体 |
| EP3456808A1 (en) | 2017-09-13 | 2019-03-20 | The Procter & Gamble Company | Automatic dishwashing cleaning composition |
| EP3467086B1 (en) | 2017-10-05 | 2021-03-24 | The Procter & Gamble Company | Dishwashing cleaning composition |
| EP3467085A1 (en) | 2017-10-05 | 2019-04-10 | The Procter & Gamble Company | Dishwashing cleaning composition |
| WO2019089898A1 (en) | 2017-11-02 | 2019-05-09 | Danisco Us Inc | Freezing point depressed solid matrix compositions for melt granulation of enzymes |
| US20200354708A1 (en) | 2017-11-29 | 2020-11-12 | Danisco Us Inc. | Subtilisin variants having improved stability |
| EP3502244B1 (en) | 2017-12-19 | 2025-03-05 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| US11834634B2 (en) | 2017-12-19 | 2023-12-05 | The Procter & Gamble Company | Phosphate-free automatic dishwashing detergent compositions having a protease and a complexing agent |
| ES3036958T3 (en) | 2017-12-19 | 2025-09-25 | Procter & Gamble | Automatic dishwashing detergent composition |
| EP3502245B1 (en) | 2017-12-19 | 2025-03-05 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| EP3502227B1 (en) | 2017-12-19 | 2024-09-04 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| BR112020012584A2 (pt) | 2017-12-21 | 2020-11-24 | Danisco Us Inc. | grânulos fundidos a quente contendo enzima que compreendem um dessecante termotolerante |
| BR112020016068A2 (pt) | 2018-02-08 | 2020-12-08 | Danisco Us Inc. | Partículas cera termicamente resistente matriz para encapsulamento de enzima |
| EP3530723B1 (en) | 2018-02-21 | 2023-03-29 | The Procter & Gamble Company | Automatic dishwashing composition |
| CN111684056A (zh) | 2018-02-28 | 2020-09-18 | 宝洁公司 | 清洁方法 |
| JP2021513590A (ja) | 2018-02-28 | 2021-05-27 | ザ プロクター アンド ギャンブル カンパニーＴｈｅ Ｐｒｏｃｔｅｒ ＆ Ｇａｍｂｌｅ Ｃｏｍｐａｎｙ | 洗浄組成物 |
| EP3799601A1 (en) | 2018-06-19 | 2021-04-07 | Danisco US Inc. | Subtilisin variants |
| US11220656B2 (en) | 2018-06-19 | 2022-01-11 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| EP3810767A1 (en) | 2018-06-19 | 2021-04-28 | Danisco US Inc. | Subtilisin variants |
| WO2019245838A1 (en) | 2018-06-19 | 2019-12-26 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| EP3830231A1 (en) | 2018-07-31 | 2021-06-09 | Danisco US Inc. | Variant alpha-amylases having amino acid substitutions that lower the pka of the general acid |
| US20210189295A1 (en) | 2018-08-30 | 2021-06-24 | Danisco Us Inc | Enzyme-containing granules |
| US20240279627A1 (en) | 2018-08-30 | 2024-08-22 | Danisco Us Inc | Compositions comprising a lipolytic enzyme variant and methods of use thereof |
| CN113166682A (zh) | 2018-09-27 | 2021-07-23 | 丹尼斯科美国公司 | 用于医疗器械清洁的组合物 |
| EP3864148A2 (en) | 2018-10-12 | 2021-08-18 | Danisco US Inc. | Alpha-amylases with mutations that improve stability in the presence of chelants |
| WO2020112599A1 (en) | 2018-11-28 | 2020-06-04 | Danisco Us Inc | Subtilisin variants having improved stability |
| CN113597469A (zh) | 2019-03-14 | 2021-11-02 | 宝洁公司 | 包含酶的清洁组合物 |
| CA3127171A1 (en) | 2019-03-14 | 2020-09-17 | The Procter & Gamble Company | Method for treating cotton |
| JP7275297B2 (ja) | 2019-03-14 | 2023-05-17 | ザ プロクター アンド ギャンブル カンパニー | 酵素を含むクリーニング組成物 |
| EP3741283B1 (en) | 2019-05-22 | 2025-03-19 | The Procter & Gamble Company | Automatic dishwashing method |
| WO2020243738A1 (en) | 2019-05-24 | 2020-12-03 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| US20220220419A1 (en) | 2019-05-24 | 2022-07-14 | Danisco Us Inc | Subtilisin variants and methods of use |
| WO2020247582A1 (en) | 2019-06-06 | 2020-12-10 | Danisco Us Inc | Methods and compositions for cleaning |
| EP3760699A1 (en) | 2019-07-02 | 2021-01-06 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| WO2021080948A2 (en) | 2019-10-24 | 2021-04-29 | Danisco Us Inc | Variant maltopentaose/maltohexaose-forming alpha-amylases |
| WO2021146255A1 (en) | 2020-01-13 | 2021-07-22 | Danisco Us Inc | Compositions comprising a lipolytic enzyme variant and methods of use thereof |
| EP3862412A1 (en) | 2020-02-04 | 2021-08-11 | The Procter & Gamble Company | Detergent composition |
| WO2021247801A1 (en) | 2020-06-05 | 2021-12-09 | The Procter & Gamble Company | Detergent compositions containing a branched surfactant |
| CA3187735A1 (en) | 2020-08-04 | 2022-02-10 | Nina Elizabeth GRAY | Automatic dishwashing method and pack |
| WO2022031311A1 (en) | 2020-08-04 | 2022-02-10 | The Procter & Gamble Company | Automatic dishwashing method |
| WO2022031309A1 (en) | 2020-08-04 | 2022-02-10 | The Procter & Gamble Company | Automatic dishwashing method |
| CA3186847A1 (en) | 2020-08-04 | 2022-02-10 | Nevena Lukic | Automatic dishwashing method |
| CN116323935A (zh) | 2020-08-27 | 2023-06-23 | 丹尼斯科美国公司 | 用于清洁的酶和酶组合物 |
| EP3974504B1 (en) | 2020-09-29 | 2023-07-26 | The Procter & Gamble Company | Automatic dishwashing cleaning composition |
| CA3196361A1 (en) | 2020-10-29 | 2022-05-05 | Catherine Jones | Cleaning compositions containing alginase enzymes |
| EP4001388A1 (en) | 2020-11-17 | 2022-05-25 | The Procter & Gamble Company | Automatic dishwashing method with amphiphilic graft polymer in the rinse |
| WO2022108766A1 (en) | 2020-11-17 | 2022-05-27 | The Procter & Gamble Company | Automatic dishwashing composition comprising amphiphilic graft polymer |
| CA3197110A1 (en) | 2020-11-17 | 2022-05-27 | Patrick Firmin August Delplancke | Automatic dishwashing method with alkaline rinse |
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| WO2022165107A1 (en) | 2021-01-29 | 2022-08-04 | Danisco Us Inc | Compositions for cleaning and methods related thereto |
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| EP4481027A1 (en) | 2023-06-19 | 2024-12-25 | The Procter & Gamble Company | Cleaning compositions containing enzymes |
| EP4488351A1 (en) | 2023-07-03 | 2025-01-08 | The Procter & Gamble Company | Compositions containing a porphyrin binding protein |
| WO2025071996A1 (en) | 2023-09-28 | 2025-04-03 | Danisco Us Inc. | Variant cutinase enzymes with improved solubility and uses thereof |
| WO2025085351A1 (en) | 2023-10-20 | 2025-04-24 | Danisco Us Inc. | Subtilisin variants and methods of use |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0571049A1 (en) * | 1988-02-11 | 1993-11-24 | Genencor International, Inc. | Novel proteolytic enzymes and their use in detergents |
| WO1994002618A1 (en) * | 1992-07-17 | 1994-02-03 | Gist-Brocades N.V. | High alkaline serine proteases |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US6287841B1 (en) * | 1988-02-11 | 2001-09-11 | Genencor International, Inc. | High alkaline serine protease |
| US5324653A (en) * | 1988-02-11 | 1994-06-28 | Gist-Brocades N.V. | Recombinant genetic means for the production of serine protease muteins |
| DK316989D0 (da) * | 1989-06-26 | 1989-06-26 | Novo Nordisk As | Enzymer |
| US5665587A (en) * | 1989-06-26 | 1997-09-09 | Novo Nordisk A/S | Modified subtilisins and detergent compositions containing same |
| US6271012B1 (en) * | 1989-10-11 | 2001-08-07 | Genencor International, Inc. | Protease muteins and their use in detergents |
| US5527483A (en) * | 1991-05-31 | 1996-06-18 | Colgate Palmolive Co. | Nonaqueous gelled automatic dishwashing composition containing enzymes |
| US5240633A (en) * | 1991-05-31 | 1993-08-31 | Colgate-Palmolive Company | Liquid automatic dishwashing composition containing enzymes |
| MA23346A1 (fr) * | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
| DE69434962T2 (de) * | 1993-10-14 | 2008-01-17 | The Procter & Gamble Company, Cincinnati | Proteasehaltige reinigungsmittel |
| AR015977A1 (es) * | 1997-10-23 | 2001-05-30 | Genencor Int | Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes |
| US6376450B1 (en) * | 1998-10-23 | 2002-04-23 | Chanchal Kumar Ghosh | Cleaning compositions containing multiply-substituted protease variants |
| US6831053B1 (en) * | 1998-10-23 | 2004-12-14 | The Procter & Gamble Company | Bleaching compositions comprising multiply-substituted protease variants |
| JP2003516751A (ja) * | 1999-12-15 | 2003-05-20 | ノボザイムス アクティーゼルスカブ | 卵の染みに及ぼす洗浄性能改良を示すズブチラーゼ変異体 |
| US6727085B2 (en) * | 1999-12-15 | 2004-04-27 | Fanoe Tina Sejersgaard | Subtilase variants having an improved wash performance on egg stains |
| US6777218B1 (en) * | 2000-03-14 | 2004-08-17 | Novozymes A/S | Subtilase enzymes having an improved wash performance on egg stains |
| US7109016B2 (en) * | 2000-08-21 | 2006-09-19 | Novozymes A/S | Subtilase enzymes |
| US6541235B1 (en) * | 2000-09-29 | 2003-04-01 | University Of Maryland Biotechnology Institute | Calcium free subtilisin mutants |
| US6893855B2 (en) * | 2000-10-13 | 2005-05-17 | Novozymes A/S | Subtilase variants |
| CA2472725C (en) * | 2002-01-16 | 2015-08-11 | Genencor International, Inc. | Multiply-substituted protease variants |
| TWI319007B (en) * | 2002-11-06 | 2010-01-01 | Novozymes As | Subtilase variants |
| WO2004099401A1 (en) * | 2003-05-07 | 2004-11-18 | Novozymes A/S | Variant subtilisin enzymes (subtilases) |
| DE102004048779A1 (de) * | 2004-10-07 | 2006-04-13 | Cognis Ip Management Gmbh | Reinigungsmittel, enthaltend Polyolhydroxyalkylether |
| MX2009000537A (es) * | 2006-07-18 | 2009-01-28 | Danisco Inc Genencor Div | Variantes de proteasa activas sobre un intervalo amplio de temperatura. |
-
2007
- 2007-07-09 MX MX2009000537A patent/MX2009000537A/es active IP Right Grant
- 2007-07-09 AU AU2007275864A patent/AU2007275864B2/en not_active Ceased
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- 2007-07-09 WO PCT/US2007/015642 patent/WO2008010925A2/en not_active Ceased
- 2007-07-09 NZ NZ573467A patent/NZ573467A/en not_active IP Right Cessation
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-
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- 2010-11-17 US US12/948,678 patent/US20110059517A1/en not_active Abandoned
-
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- 2013-04-25 US US13/870,798 patent/US20130323816A1/en not_active Abandoned
-
2014
- 2014-04-07 US US14/246,356 patent/US20140302590A1/en not_active Abandoned
-
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- 2015-09-30 JP JP2015193074A patent/JP2016052308A/ja active Pending
-
2018
- 2018-05-15 US US15/979,671 patent/US20190078070A1/en not_active Abandoned
-
2019
- 2019-07-23 US US16/519,232 patent/US20190338267A1/en not_active Abandoned
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0571049A1 (en) * | 1988-02-11 | 1993-11-24 | Genencor International, Inc. | Novel proteolytic enzymes and their use in detergents |
| RU2069695C1 (ru) * | 1988-02-11 | 1996-11-27 | Гист-Брокейдс Н.В. | Способ получения мутантной протеазы |
| WO1994002618A1 (en) * | 1992-07-17 | 1994-02-03 | Gist-Brocades N.V. | High alkaline serine proteases |
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| BRPI0714329B1 (pt) | 2017-09-12 |
| WO2008010925A2 (en) | 2008-01-24 |
| AU2007275864A1 (en) | 2008-01-24 |
| US20080090747A1 (en) | 2008-04-17 |
| KR20090031906A (ko) | 2009-03-30 |
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| US20110059517A1 (en) | 2011-03-10 |
| DK2059591T3 (da) | 2012-10-29 |
| EP2059591B1 (en) | 2012-09-05 |
| AU2007275864B2 (en) | 2013-05-02 |
| CA2658396A1 (en) | 2008-01-24 |
| RU2009105503A (ru) | 2010-08-27 |
| JP2016052308A (ja) | 2016-04-14 |
| US20190078070A1 (en) | 2019-03-14 |
| US20130323816A1 (en) | 2013-12-05 |
| NZ573467A (en) | 2012-02-24 |
| US20190338267A1 (en) | 2019-11-07 |
| JP2009543577A (ja) | 2009-12-10 |
| CN101511998A (zh) | 2009-08-19 |
| US20140302590A1 (en) | 2014-10-09 |
| MX2009000537A (es) | 2009-01-28 |
| WO2008010925A3 (en) | 2008-04-10 |
| BRPI0714329A2 (pt) | 2013-03-05 |
| CA2658396C (en) | 2019-07-16 |
| KR20150115023A (ko) | 2015-10-13 |
| CN104232365A (zh) | 2014-12-24 |
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