RU2016129161A - Усовершенствованный диагностический тест для csfv антител - Google Patents
Усовершенствованный диагностический тест для csfv антител Download PDFInfo
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- RU2016129161A RU2016129161A RU2016129161A RU2016129161A RU2016129161A RU 2016129161 A RU2016129161 A RU 2016129161A RU 2016129161 A RU2016129161 A RU 2016129161A RU 2016129161 A RU2016129161 A RU 2016129161A RU 2016129161 A RU2016129161 A RU 2016129161A
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- csfv
- tavspttlr
- epitope
- mutant
- carrier
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- 238000002405 diagnostic procedure Methods 0.000 title claims 5
- LXWYCLOUQZZDBD-LIYNQYRNSA-N csfv Chemical compound C([C@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)[C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(O)=O)C1=CC=C(O)C=C1 LXWYCLOUQZZDBD-LIYNQYRNSA-N 0.000 title 1
- 238000000034 method Methods 0.000 claims 15
- 229960005486 vaccine Drugs 0.000 claims 6
- 101710125507 Integrase/recombinase Proteins 0.000 claims 5
- 238000011534 incubation Methods 0.000 claims 4
- 238000002965 ELISA Methods 0.000 claims 2
- 241001465754 Metazoa Species 0.000 claims 2
- 241000282898 Sus scrofa Species 0.000 claims 2
- 238000001514 detection method Methods 0.000 claims 2
- 241000710777 Classical swine fever virus Species 0.000 claims 1
- 241000282887 Suidae Species 0.000 claims 1
- 241000700605 Viruses Species 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 239000000969 carrier Substances 0.000 claims 1
- 208000015181 infectious disease Diseases 0.000 claims 1
- 230000001404 mediated effect Effects 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
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- G—PHYSICS
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- A61K39/00—Medicinal preparations containing antigens or antibodies
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Claims (12)
1. Способ обнаружения антител против вируса классической чумы свиней (CSFV) дикого типа в тестируемом образце, где указанный образец также может содержать антитела против мутантного эпитопа TAVSPTTLR из CSFV E2, способ включает стадию инкубации указанного тестового образца с иммобилизованным носителем, который содержит эпитоп TAVSPTTLR из CSFV E2, который отличается тем, что этот способ включает совместную инкубацию на указанной стадии с носителем, который содержит мутантный эпитоп TAVSPTTLR из CSFV E2.
2. Способ по п.1, в котором обнаружение опосредовано ELISA (твердофазным иммуноферментным анализом).
3. Способ по пп.1 или 2, в котором тестовый образец получают от животного свиньи, которое вакцинировали против CSF с использованием CSFV вакцины, которая содержит белок E2 CSFV, содержащий мутантный эпитоп TAVSPTTLR.
4. Способ по любому одному из пп.1-3, в котором мутантный эпитоп TAVSPTTLR из CSFV E2, который содержится в носителе для использования при совместной инкубации, является таким же, как мутантный эпитоп TAVSPTTLR, содержащийся в белке E2 CSFV из CSFV вакцины, которую использовали для того, чтобы вакцинировать животных свиней, от которых получали тестовые образцы.
5. Способ по любому одному из пп.1-4, в котором мутантный эпитоп TAVSPTTLR из CSFV E2, который содержится в носителе для использования при совместной инкубации, имеет аминокислотную последовательность: TAGSTLRTE (SEQ ID № 2).
6. Способ по п.4 или 5, в котором CSFV вакцина основана на вирусе vFlc-ΔPTa1.
7. Способ по любому одному из пп.1-6, в котором или иммобилизованный носитель, который содержит эпитоп TAVSPTTLR из CSFV E2, или носитель, который содержит мутантный эпитоп TAVSPTTLR из CSFV E2, представляет собой белок E2 CSFV или в котором оба носителя представляют собой белки E2 CSFV.
8. Способ по любому одному из пп.1-7, который включает адаптацию условий инкубации на стадии, включающей совместную инкубацию с носителем, который содержит мутантный эпитоп TAVSPTTLR из CSFV E2, чтобы приспосабливать ее к добавлению указанного носителя.
9. Набор для диагностического теста для осуществления способа по любому одному из пп.1-8.
10. Использование носителя, который содержит мутантный эпитоп TAVSPTTLR из CSFV E2, для совместной инкубации в способе по любому одному из пп.1-8 или в наборе для диагностического теста по п.9.
11. Способ дифференциации между животными, инфицированными CSFV дикого типа, и животными, которых вакцинировали против CSFV с использованием CSFV вакцины, где вакцина содержит белок E2 CSFV, содержащий мутантный эпитоп TAVSPTTLR, и способ включает использование способа обнаружения по любому одному из пп.1-8 или включает использование набора для диагностического теста по п.9.
12. Способ управления инфекцией CSFV дикого типа в популяции животных свиней посредством комбинированного использования CSFV вакцины, которая содержит белок E2 CSFV, содержащий мутантный эпитоп TAVSPTTLR*, и набора для диагностического теста по п.9.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13198615 | 2013-12-19 | ||
| EP13198615.0 | 2013-12-19 | ||
| PCT/EP2014/078373 WO2015091736A1 (en) | 2013-12-19 | 2014-12-18 | Improved diagnostic test for csfv antibodies |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2016129161A true RU2016129161A (ru) | 2018-01-24 |
| RU2689143C1 RU2689143C1 (ru) | 2019-05-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| RU2016129161A RU2689143C1 (ru) | 2013-12-19 | 2014-12-18 | Усовершенствованный диагностический тест для csfv антител |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US9739778B2 (ru) |
| EP (1) | EP3084441B1 (ru) |
| JP (1) | JP6383422B2 (ru) |
| KR (1) | KR101848194B1 (ru) |
| CN (1) | CN105829892B (ru) |
| BR (1) | BR112016014010B1 (ru) |
| ES (1) | ES2668459T3 (ru) |
| HU (1) | HUE038721T2 (ru) |
| RU (1) | RU2689143C1 (ru) |
| TW (1) | TWI611188B (ru) |
| WO (1) | WO2015091736A1 (ru) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101848194B1 (ko) * | 2013-12-19 | 2018-04-11 | 인터벳 인터내셔널 비.브이. | Csfv 항체에 대한 개선된 진단 시험 |
| CN106383226A (zh) * | 2016-08-19 | 2017-02-08 | 金宇保灵生物药品有限公司 | 猪血清中猪瘟中和抗体效价的定量检测方法及检测试剂盒 |
| CN107064501B (zh) * | 2017-05-02 | 2019-04-16 | 中国农业科学院兰州兽医研究所 | 一种定量检测猪瘟病毒IgG抗体竞争ELISA试剂盒及其检测方法 |
| CN107828745B (zh) * | 2017-11-15 | 2021-01-05 | 中国兽医药品监察所 | 猪瘟兔化弱毒表位突变株及其应用 |
| EP3956439A4 (en) * | 2019-04-18 | 2023-05-03 | Boehringer Ingelheim Vetmedica (China) Co., Ltd. | RECOMBINANT CLASSICAL SWINE FEVER VIRUS |
| CN111044494B (zh) * | 2019-11-26 | 2022-08-23 | 安徽云燕食品科技有限公司 | 一种猪瘟的快速检测方法 |
| CN112375126B (zh) * | 2020-11-04 | 2022-07-29 | 天康生物制药有限公司 | 标记猪瘟病毒e2蛋白重组杆状病毒灭活疫苗 |
| CN114236128B (zh) * | 2021-11-30 | 2022-07-29 | 中国农业科学院兰州兽医研究所 | 一种检测猪急性腹泻综合征冠状病毒n蛋白抗体的阻断elisa试剂盒 |
| CN116068175B (zh) * | 2022-09-07 | 2024-06-07 | 中国农业科学院兰州兽医研究所 | 一种基于e2蛋白二聚体的猪瘟病毒管式化学发光抗体检测试剂盒及其应用 |
| CN116953234B (zh) * | 2023-08-25 | 2024-05-17 | 中国农业科学院兰州兽医研究所 | 一种猪博卡病毒g3型的多肽-elisa抗体检测试剂盒 |
| CN117510623A (zh) * | 2023-11-09 | 2024-02-06 | 青岛农业大学 | 一种CSFV E0蛋白的纳米抗体CSFV-E0-Nb1及编码基因和应用 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| HU220746B1 (hu) * | 1994-06-17 | 2002-05-28 | Instituut Voor Veehouderij En Diergezondheid | Pestivírustörzsekből izolált nukleotidszekvenciák, az általuk kódolt polipeptidek és ezek alkalmazása pestivírus-fertőzések diagnosztizálására és megelőzésére |
| US7763460B2 (en) * | 2005-06-24 | 2010-07-27 | Focus Diagnostics, Inc. | Methods and compositions for detecting herpes simplex virus type 2 |
| US8846055B2 (en) | 2006-05-30 | 2014-09-30 | The United States Of America, As Represented By The Secretary Of Agriculture | Virulence determinant within the E2 structural glycoprotein of classical swine fever virus |
| CN101144818B (zh) * | 2007-10-19 | 2011-04-27 | 清华大学 | 检测猪瘟病毒特异性抗体的方法及其酶联免疫试剂盒 |
| EP2127678A1 (en) * | 2008-05-26 | 2009-12-02 | Roche Diagnostics GmbH | SlpA as a tool for recombinant protein and enzyme technology |
| JP2012502641A (ja) * | 2008-09-17 | 2012-02-02 | インターベツト・インターナシヨナル・ベー・ベー | コア遺伝子およびｎｓ３領域に突然変異を有する突然変異ペスチウイルス |
| EP2202298A1 (en) | 2008-12-23 | 2010-06-30 | Stichting Dienst Landbouwkundig Onderzoek | Recombinant classical swine fever virus (CSFV) comprising a modified E2 protein and methods for generating said recombinant CSFV |
| US9291624B2 (en) * | 2011-08-24 | 2016-03-22 | Zoetis Services Llc | Vaccine diagnostics |
| KR101848194B1 (ko) * | 2013-12-19 | 2018-04-11 | 인터벳 인터내셔널 비.브이. | Csfv 항체에 대한 개선된 진단 시험 |
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- 2014-12-18 KR KR1020167016192A patent/KR101848194B1/ko active IP Right Grant
- 2014-12-18 BR BR112016014010-9A patent/BR112016014010B1/pt active IP Right Grant
- 2014-12-18 CN CN201480069404.4A patent/CN105829892B/zh active Active
- 2014-12-18 JP JP2016540569A patent/JP6383422B2/ja active Active
- 2014-12-18 ES ES14815702.7T patent/ES2668459T3/es active Active
- 2014-12-18 TW TW103144388A patent/TWI611188B/zh active
- 2014-12-18 HU HUE14815702A patent/HUE038721T2/hu unknown
- 2014-12-18 EP EP14815702.7A patent/EP3084441B1/en active Active
- 2014-12-18 US US15/104,406 patent/US9739778B2/en active Active
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Also Published As
| Publication number | Publication date |
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| WO2015091736A1 (en) | 2015-06-25 |
| JP2017503165A (ja) | 2017-01-26 |
| TW201530141A (zh) | 2015-08-01 |
| EP3084441A1 (en) | 2016-10-26 |
| US20160313330A1 (en) | 2016-10-27 |
| TWI611188B (zh) | 2018-01-11 |
| US9739778B2 (en) | 2017-08-22 |
| BR112016014010B1 (pt) | 2022-08-30 |
| KR101848194B1 (ko) | 2018-04-11 |
| CN105829892A (zh) | 2016-08-03 |
| ES2668459T3 (es) | 2018-05-18 |
| HUE038721T2 (hu) | 2018-11-28 |
| JP6383422B2 (ja) | 2018-08-29 |
| EP3084441B1 (en) | 2018-03-28 |
| CN105829892B (zh) | 2018-06-12 |
| KR20160097219A (ko) | 2016-08-17 |
| BR112016014010A2 (pt) | 2018-01-09 |
| RU2689143C1 (ru) | 2019-05-24 |
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