RU2011123739A - Протеазы, содержащие одну или несколько комбинируемых мутаций - Google Patents
Протеазы, содержащие одну или несколько комбинируемых мутаций Download PDFInfo
- Publication number
- RU2011123739A RU2011123739A RU2011123739/10A RU2011123739A RU2011123739A RU 2011123739 A RU2011123739 A RU 2011123739A RU 2011123739/10 A RU2011123739/10 A RU 2011123739/10A RU 2011123739 A RU2011123739 A RU 2011123739A RU 2011123739 A RU2011123739 A RU 2011123739A
- Authority
- RU
- Russia
- Prior art keywords
- subtilisin
- positions
- variant
- detergent
- seq
- Prior art date
Links
- 108091005804 Peptidases Proteins 0.000 title claims 2
- 102000035195 Peptidases Human genes 0.000 title claims 2
- 239000004365 Protease Substances 0.000 title claims 2
- 230000035772 mutation Effects 0.000 title 1
- 108090000787 Subtilisin Proteins 0.000 claims abstract 21
- 239000003599 detergent Substances 0.000 claims abstract 10
- 238000004140 cleaning Methods 0.000 claims abstract 7
- 238000006467 substitution reaction Methods 0.000 claims abstract 6
- 239000013604 expression vector Substances 0.000 claims abstract 4
- 102000039446 nucleic acids Human genes 0.000 claims abstract 4
- 108020004707 nucleic acids Proteins 0.000 claims abstract 4
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 4
- 238000005406 washing Methods 0.000 claims abstract 3
- 102220465972 Cilium assembly protein DZIP1_S24R_mutation Human genes 0.000 claims abstract 2
- 102220545278 Desmoglein-1_S24E_mutation Human genes 0.000 claims abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 2
- 230000000694 effects Effects 0.000 claims abstract 2
- 239000007788 liquid Substances 0.000 claims abstract 2
- 102000004169 proteins and genes Human genes 0.000 claims abstract 2
- 108090000623 proteins and genes Proteins 0.000 claims abstract 2
- 230000002797 proteolythic effect Effects 0.000 claims abstract 2
- 102200143804 rs121434229 Human genes 0.000 claims abstract 2
- 102220270302 rs1416460262 Human genes 0.000 claims abstract 2
- 102220059026 rs786201684 Human genes 0.000 claims abstract 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims 3
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 108090000854 Oxidoreductases Proteins 0.000 claims 2
- 102000004316 Oxidoreductases Human genes 0.000 claims 2
- 108010056079 Subtilisins Proteins 0.000 claims 2
- 102000005158 Subtilisins Human genes 0.000 claims 2
- 229940088598 enzyme Drugs 0.000 claims 2
- 108010065511 Amylases Proteins 0.000 claims 1
- 102000013142 Amylases Human genes 0.000 claims 1
- 108700038091 Beta-glucanases Proteins 0.000 claims 1
- 102100032487 Beta-mannosidase Human genes 0.000 claims 1
- 102000005575 Cellulases Human genes 0.000 claims 1
- 108010084185 Cellulases Proteins 0.000 claims 1
- 108010023736 Chondroitinases and Chondroitin Lyases Proteins 0.000 claims 1
- 102000011413 Chondroitinases and Chondroitin Lyases Human genes 0.000 claims 1
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims 1
- 108090000371 Esterases Proteins 0.000 claims 1
- 108050009363 Hyaluronidases Proteins 0.000 claims 1
- 102000001974 Hyaluronidases Human genes 0.000 claims 1
- 108090001060 Lipase Proteins 0.000 claims 1
- 102000004882 Lipase Human genes 0.000 claims 1
- 239000004367 Lipase Substances 0.000 claims 1
- 108090000128 Lipoxygenases Proteins 0.000 claims 1
- 102000003820 Lipoxygenases Human genes 0.000 claims 1
- 108010006035 Metalloproteases Proteins 0.000 claims 1
- 102000005741 Metalloproteases Human genes 0.000 claims 1
- 108700020962 Peroxidase Proteins 0.000 claims 1
- 102000003992 Peroxidases Human genes 0.000 claims 1
- 108010064785 Phospholipases Proteins 0.000 claims 1
- 102000015439 Phospholipases Human genes 0.000 claims 1
- 108010059820 Polygalacturonase Proteins 0.000 claims 1
- 108091007187 Reductases Proteins 0.000 claims 1
- 102000003425 Tyrosinase Human genes 0.000 claims 1
- 108060008724 Tyrosinase Proteins 0.000 claims 1
- 108090000637 alpha-Amylases Proteins 0.000 claims 1
- 108010084650 alpha-N-arabinofuranosidase Proteins 0.000 claims 1
- 235000019418 amylase Nutrition 0.000 claims 1
- 229940025131 amylases Drugs 0.000 claims 1
- 108010055059 beta-Mannosidase Proteins 0.000 claims 1
- 108010005400 cutinase Proteins 0.000 claims 1
- 238000004851 dishwashing Methods 0.000 claims 1
- 108010093305 exopolygalacturonase Proteins 0.000 claims 1
- 239000004744 fabric Substances 0.000 claims 1
- 108010002430 hemicellulase Proteins 0.000 claims 1
- 239000004615 ingredient Substances 0.000 claims 1
- 108010059345 keratinase Proteins 0.000 claims 1
- 108010062085 ligninase Proteins 0.000 claims 1
- 235000019421 lipase Nutrition 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 108010087558 pectate lyase Proteins 0.000 claims 1
- -1 perhydrolases Proteins 0.000 claims 1
- 239000003381 stabilizer Substances 0.000 claims 1
- 108010038851 tannase Proteins 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38609—Protease or amylase in solid compositions only
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38618—Protease or amylase in liquid compositions only
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38654—Preparations containing enzymes, e.g. protease or amylase containing oxidase or reductase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38663—Stabilised liquid enzyme compositions
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38681—Chemically modified or immobilised enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21062—Subtilisin (3.4.21.62)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Detergent Compositions (AREA)
Abstract
1. Выделенный вариант субтилизина, который является зрелой формой, обладающей протеолитической активностью и включающей замену в двух или более положениях, выбираемых из положений 24, 45, 101, 109, 118, 213 и 217, нумерация которых соответствует аминокислотной последовательности субтилизина BPN' B. amyloliquefaciens, представленной как SEQ ID NO:1.2. Выделенный вариант субтилизина по п.1, который имеет относительный индекс продуктивности уровня экспрессии белка (ТСА PI) и/или индекс продуктивности активности по удалению пятен (BMI PI), больший или равный 0,5.3. Выделенный вариант субтилизина по п.1 или 2, где указанный субтилизинпредставляет собой GG36 и где указанная замена в двух или более положениях выбрана из S24Q, S24E, S24L, S24R, R45Q, R45E, R45L, S101Q, S101E, S101L, S101R, Q109E, Q109L, Q109R, G118Q, G118E, G118L, G118R, T213Q, T213L, T213R, T213E, L217Q и L217E, соответствующих положениям субтилизина BPN' SEQ ID NO:1.4. Выделенный вариант субтилизина по любому из п.1 или 2, где указанный субтилизинпредставляет собой GG36 и где указанная замена в двух или более положениях представляет собой S24Q-R45Q-S101Q-G118Q-T213Q, соответствующую положениям субтилизина BPN' SEQ ID NO:1.5. Выделенная нуклеиновая кислота, кодирующая вариант субтилизина по любому из пп.1-4.6. Экспрессирующий вектор, содержащий нуклеиновую кислоту по п.5.7. Клетка-хозяин, содержащая экспрессирующий вектор по п.6.8. Очищающая композиция, содержащая по меньшей мере один вариант субтилизина по любому из пп.1-4.9. Очищающая композиция по п.8, которая является детергентом.10. Очищающая композиция по п.9, в которой указанный детергент является сверхсильным жидким или сухим стиральным детергентом.11. Очищающая композиция по п.9, в которой указанный детергент является детергентом для мыть�
Claims (15)
1. Выделенный вариант субтилизина Bacillus, который является зрелой формой, обладающей протеолитической активностью и включающей замену в двух или более положениях, выбираемых из положений 24, 45, 101, 109, 118, 213 и 217, нумерация которых соответствует аминокислотной последовательности субтилизина BPN' B. amyloliquefaciens, представленной как SEQ ID NO:1.
2. Выделенный вариант субтилизина по п.1, который имеет относительный индекс продуктивности уровня экспрессии белка (ТСА PI) и/или индекс продуктивности активности по удалению пятен (BMI PI), больший или равный 0,5.
3. Выделенный вариант субтилизина по п.1 или 2, где указанный субтилизин Bacillus представляет собой GG36 и где указанная замена в двух или более положениях выбрана из S24Q, S24E, S24L, S24R, R45Q, R45E, R45L, S101Q, S101E, S101L, S101R, Q109E, Q109L, Q109R, G118Q, G118E, G118L, G118R, T213Q, T213L, T213R, T213E, L217Q и L217E, соответствующих положениям субтилизина BPN' SEQ ID NO:1.
4. Выделенный вариант субтилизина по любому из п.1 или 2, где указанный субтилизин Bacillus представляет собой GG36 и где указанная замена в двух или более положениях представляет собой S24Q-R45Q-S101Q-G118Q-T213Q, соответствующую положениям субтилизина BPN' SEQ ID NO:1.
5. Выделенная нуклеиновая кислота, кодирующая вариант субтилизина по любому из пп.1-4.
6. Экспрессирующий вектор, содержащий нуклеиновую кислоту по п.5.
7. Клетка-хозяин, содержащая экспрессирующий вектор по п.6.
8. Очищающая композиция, содержащая по меньшей мере один вариант субтилизина по любому из пп.1-4.
9. Очищающая композиция по п.8, которая является детергентом.
10. Очищающая композиция по п.9, в которой указанный детергент является сверхсильным жидким или сухим стиральным детергентом.
11. Очищающая композиция по п.9, в которой указанный детергент является детергентом для мытья посуды.
12. Очищающая композиция по любому из пп.8-11, дополнительно содержащая один или несколько дополнительных ферментов или производных ферментов, выбираемых из гемицеллюлаз, целлюлаз, пероксидаз, протеаз, металлопротеаз, ксиланаз, липаз, фосфолипаз, эстераз, пергидролаз, кутиназ, пектиназ, пектат-лиаз, маннаназ, кератиназ, редуктаз, оксидаз, фенолоксидаз, липоксигеназ, лигниназ, пуллуланаз, танназ, пентозаназ, маланаз, β-глюканаз, арабинозидаз, гиалуронидаз, хондроитиназ, лакказ и амилаз или их смесей.
13. Очищающая композиция по любому из пп.8-11, дополнительно содержащая по меньшей мере один стабилизатор.
14. Очищающая композиция, содержащая по меньшей мере 0,0001 мас.% по меньшей мере одного варианта субтилизина по любому из пп.1-4 и необязательно по меньшей мере один приемлемый дополнительный ингредиент.
15. Способ очистки, который включает стадии:
а) контактирования поверхности и/или изделия, содержащего ткань, с очищающей композицией по любому из пп.7-14; и
b) необязательно промывки и/или прополаскивания указанной поверхности или изделия.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11354508P | 2008-11-11 | 2008-11-11 | |
| US61/113,545 | 2008-11-11 | ||
| US21880209P | 2009-06-19 | 2009-06-19 | |
| US61/218,802 | 2009-06-19 | ||
| PCT/US2009/063837 WO2010056653A2 (en) | 2008-11-11 | 2009-11-10 | Proteases comprising one or more combinable mutations |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2011123739A true RU2011123739A (ru) | 2012-12-20 |
| RU2560978C2 RU2560978C2 (ru) | 2015-08-20 |
Family
ID=41786712
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2011123739/10A RU2560978C2 (ru) | 2008-11-11 | 2009-11-10 | Протеазы, содержащие одну или несколько комбинируемых мутаций |
Country Status (11)
| Country | Link |
|---|---|
| US (4) | US8753861B2 (ru) |
| EP (3) | EP3031894A1 (ru) |
| JP (3) | JP2012508031A (ru) |
| KR (1) | KR20110095260A (ru) |
| CN (4) | CN102209775A (ru) |
| AR (1) | AR074311A1 (ru) |
| BR (2) | BR122013014157A2 (ru) |
| CA (1) | CA2743302A1 (ru) |
| MX (1) | MX2011004802A (ru) |
| RU (1) | RU2560978C2 (ru) |
| WO (1) | WO2010056653A2 (ru) |
Families Citing this family (120)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK1523553T3 (da) * | 2002-01-16 | 2010-04-19 | Genencor Int | Multi-substituerede proteasevarianter |
| US20090060933A1 (en) * | 2004-06-14 | 2009-03-05 | Estell David A | Proteases producing an altered immunogenic response and methods of making and using the same |
| WO2007122175A1 (en) * | 2006-04-20 | 2007-11-01 | Novozymes A/S | Savinase variants having an improved wash performance on egg stains |
| EP3031894A1 (en) | 2008-11-11 | 2016-06-15 | Danisco US Inc. | Proteases comprising one or more combinable mutations |
| BRPI0922084B1 (pt) * | 2008-11-11 | 2020-12-29 | Danisco Us Inc. | variante de subtilisina isolada de uma subtilisina de bacillus e sua composição de limpeza |
| US20110281327A1 (en) * | 2010-04-15 | 2011-11-17 | Danisco Us Inc. | Compositions And Methods Comprising Variant Proteases |
| BR112012028467B1 (pt) * | 2010-05-06 | 2021-02-17 | Danisco Us Inc. | variantes de subtilisina, seu método de produção, ácido nucleico, e método de limpeza |
| CN103255187B (zh) * | 2012-02-21 | 2015-02-18 | 华熙福瑞达生物医药有限公司 | 一种低分子透明质酸盐、其制备方法及用途 |
| WO2014099525A1 (en) | 2012-12-21 | 2014-06-26 | Danisco Us Inc. | Paenibacillus curdlanolyticus amylase, and methods of use, thereof |
| US20160053243A1 (en) | 2012-12-21 | 2016-02-25 | Danisco Us Inc. | Alpha-amylase variants |
| EP2970929A1 (en) | 2013-03-11 | 2016-01-20 | Danisco US Inc. | Alpha-amylase combinatorial variants |
| CN111394202B (zh) * | 2013-04-23 | 2022-04-26 | 诺维信公司 | 具有稳定的枯草杆菌蛋白酶的液体自动餐具清洗洗涤剂组合物 |
| EP3004314B1 (en) | 2013-05-29 | 2018-06-20 | Danisco US Inc. | Novel metalloproteases |
| EP3004342B1 (en) | 2013-05-29 | 2023-01-11 | Danisco US Inc. | Novel metalloproteases |
| DK3110833T3 (da) | 2013-05-29 | 2020-04-06 | Danisco Us Inc | Hidtil ukendte metalloproteaser |
| US20160108388A1 (en) | 2013-05-29 | 2016-04-21 | Danisco Us Inc. | Novel metalloproteases |
| CN104419651B (zh) * | 2013-08-19 | 2018-12-25 | 丰益(上海)生物技术研发中心有限公司 | 磷脂酶c及其产生菌株 |
| DE102013218253A1 (de) | 2013-09-12 | 2015-03-12 | Henkel Ag & Co. Kgaa | Festes Textilwaschmittel mit verbesserter Proteaseleistung |
| CN104560763B (zh) * | 2013-10-15 | 2018-12-25 | 丰益(上海)生物技术研发中心有限公司 | 产磷脂酶c的地衣芽孢杆菌及其产生的磷脂酶c |
| CN105705622A (zh) | 2013-10-15 | 2016-06-22 | 丹尼斯科美国公司 | 粘土粒料 |
| CN104560764B (zh) * | 2013-10-17 | 2018-12-25 | 丰益(上海)生物技术研发中心有限公司 | 产磷脂酶c的短小芽孢杆菌及其产生的磷脂酶c |
| AU2014346511B2 (en) | 2013-11-11 | 2016-12-22 | Ecolab Usa Inc. | Multiuse, enzymatic detergent and methods of stabilizing a use solution |
| BR112016010425B8 (pt) | 2013-11-11 | 2023-01-31 | Ecolab Usa Inc | Detergente concentrado para lavar utensílios e seu método de utilização |
| EP3068879B1 (en) | 2013-11-14 | 2019-12-25 | Danisco US Inc. | Stable enzymes by glycation reduction |
| TR201906371T4 (tr) | 2013-12-13 | 2019-05-21 | Danisco Inc | Bacillus türlerinin serin proteazları. |
| EP3080263B1 (en) | 2013-12-13 | 2019-07-03 | Danisco US Inc. | Serine proteases of the bacillus gibsonii-clade |
| MX373205B (es) | 2013-12-16 | 2020-05-04 | Nutrition & Biosciences Usa 4 Inc | Uso de eteres de poli-alfa-1,3-glucano como modificadores de la viscosidad. |
| BR112016014014B1 (pt) | 2013-12-18 | 2021-08-10 | Nutrition & Biosciences USA 4, Inc. | Composição, método para a produção de um composto e método para o tratamento de um material |
| EP3097175B1 (en) | 2014-01-22 | 2018-10-17 | The Procter and Gamble Company | Fabric treatment composition |
| EP3097174A1 (en) | 2014-01-22 | 2016-11-30 | The Procter & Gamble Company | Method of treating textile fabrics |
| EP3097172A1 (en) | 2014-01-22 | 2016-11-30 | The Procter & Gamble Company | Method of treating textile fabrics |
| WO2015112339A1 (en) | 2014-01-22 | 2015-07-30 | The Procter & Gamble Company | Fabric treatment composition |
| EP3105256A1 (en) | 2014-02-14 | 2016-12-21 | E. I. du Pont de Nemours and Company | Poly-alpha-1,3-1,6-glucans for viscosity modification |
| CA2937830A1 (en) | 2014-03-11 | 2015-09-17 | E. I. Du Pont De Nemours And Company | Oxidized poly alpha-1,3-glucan as detergent builder |
| EP4155398A1 (en) | 2014-03-21 | 2023-03-29 | Danisco US Inc. | Serine proteases of bacillus species |
| WO2015195777A1 (en) | 2014-06-19 | 2015-12-23 | E. I. Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
| US9714403B2 (en) | 2014-06-19 | 2017-07-25 | E I Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
| WO2016061438A1 (en) | 2014-10-17 | 2016-04-21 | Danisco Us Inc. | Serine proteases of bacillus species |
| US20170306360A1 (en) | 2014-10-24 | 2017-10-26 | Danisco Us Inc. | Method for producing alcohol by use of a tripeptidyl peptidase |
| WO2016069544A1 (en) | 2014-10-27 | 2016-05-06 | Danisco Us Inc. | Serine proteases |
| EP3212662B1 (en) | 2014-10-27 | 2020-04-08 | Danisco US Inc. | Serine proteases |
| EP3212781B1 (en) | 2014-10-27 | 2019-09-18 | Danisco US Inc. | Serine proteases |
| EP3224357A1 (en) | 2014-10-27 | 2017-10-04 | Danisco US Inc. | Serine proteases of bacillus species |
| WO2016069569A2 (en) | 2014-10-27 | 2016-05-06 | Danisco Us Inc. | Serine proteases |
| CA2967658A1 (en) | 2014-11-17 | 2016-05-26 | The Procter & Gamble Company | Benefit agent delivery compositions |
| DK3399031T3 (da) * | 2014-12-15 | 2020-01-20 | Henkel Ag & Co Kgaa | Detergentsammensætning med subtilasevarianter |
| WO2016096711A2 (en) | 2014-12-15 | 2016-06-23 | Novozymes A/S | Subtilase variants |
| EP3237632B8 (en) | 2014-12-23 | 2019-03-13 | E.I. Du Pont De Nemours And Company | Enzymatically produced water-insoluble cellulose |
| EP3088505B1 (en) | 2015-04-29 | 2020-06-03 | The Procter and Gamble Company | Method of treating a fabric |
| EP3088502B1 (en) | 2015-04-29 | 2018-05-23 | The Procter and Gamble Company | Method of treating a fabric |
| EP3088504B1 (en) | 2015-04-29 | 2021-07-21 | The Procter & Gamble Company | Method of treating a fabric |
| PL3088503T3 (pl) | 2015-04-29 | 2018-10-31 | The Procter & Gamble Company | Sposób prania tkanin |
| DK3088506T3 (en) | 2015-04-29 | 2018-08-13 | Procter & Gamble | detergent |
| RU2733987C2 (ru) | 2015-05-13 | 2020-10-09 | ДАНИСКО ЮЭс ИНК. | Варианты протеазы клады aprl и их применения |
| US20180134997A1 (en) | 2015-06-09 | 2018-05-17 | Danisco Us Inc. | Osmotic burst encapsulates |
| WO2016201040A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc. | Water-triggered enzyme suspension |
| WO2016201069A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc | Low-density enzyme-containing particles |
| US11499146B2 (en) | 2015-06-17 | 2022-11-15 | Danisco Us Inc. | Bacillus gibsonii-clade serine proteases |
| US10894954B2 (en) | 2015-07-06 | 2021-01-19 | Uvic Industry Partnerships Inc. | Subtilisin variants and uses thereof |
| EP3371308B1 (en) | 2015-11-05 | 2022-05-11 | Danisco US Inc. | Paenibacillus sp. mannanases |
| WO2017079756A1 (en) | 2015-11-05 | 2017-05-11 | Danisco Us Inc | Paenibacillus and bacillus spp. mannanases |
| EP3374488B1 (en) | 2015-11-13 | 2020-10-14 | DuPont Industrial Biosciences USA, LLC | Glucan fiber compositions for use in laundry care and fabric care |
| EP3374400B1 (en) | 2015-11-13 | 2022-04-13 | Nutrition & Biosciences USA 4, Inc. | Glucan fiber compositions for use in laundry care and fabric care |
| WO2017083229A1 (en) | 2015-11-13 | 2017-05-18 | E. I. Du Pont De Nemours And Company | Glucan fiber compositions for use in laundry care and fabric care |
| CN108291179B (zh) | 2015-11-25 | 2020-07-07 | 荷兰联合利华有限公司 | 液体洗涤剂组合物 |
| US11920170B2 (en) | 2015-12-09 | 2024-03-05 | Danisco Us Inc. | Alpha-amylase combinatorial variants |
| EP3390625B1 (en) | 2015-12-18 | 2023-09-06 | Danisco US Inc. | Polypeptides with endoglucanase activity and uses thereof |
| US10806769B2 (en) | 2016-03-31 | 2020-10-20 | Gojo Industries, Inc. | Antimicrobial peptide stimulating cleansing composition |
| EP3435765A1 (en) | 2016-03-31 | 2019-02-06 | Gojo Industries, Inc. | Antimicrobial peptide stimulating sanitizing composition |
| EP3436041B1 (en) | 2016-03-31 | 2023-05-24 | Gojo Industries, Inc. | Sanitizer composition with probiotic/prebiotic active ingredient |
| DE102016205670A1 (de) * | 2016-04-06 | 2017-10-12 | Henkel Ag & Co. Kgaa | Neue Protease mit verbesserter Waschleistung |
| EP3464599A1 (en) | 2016-05-31 | 2019-04-10 | Danisco US Inc. | Protease variants and uses thereof |
| BR112018075933A2 (pt) | 2016-06-17 | 2019-10-01 | Danisco Us Inc | variantes de protease e usos das mesmas |
| US20190264138A1 (en) | 2016-11-07 | 2019-08-29 | Danisco Us Inc. | Laundry detergent composition |
| JP2020500860A (ja) | 2016-11-23 | 2020-01-16 | ゴジョ・インダストリーズ・インコーポレイテッド | プロバイオティック／プレバイオティックな有効成分を含む消毒薬組成物 |
| US10550443B2 (en) | 2016-12-02 | 2020-02-04 | The Procter & Gamble Company | Cleaning compositions including enzymes |
| EP3330349B1 (en) | 2016-12-02 | 2024-06-19 | The Procter & Gamble Company | Cleaning compositions including enzymes |
| JP6907318B2 (ja) | 2016-12-02 | 2021-07-21 | ザ プロクター アンド ギャンブル カンパニーＴｈｅ Ｐｒｏｃｔｅｒ ＆ Ｇａｍｂｌｅ Ｃｏｍｐａｎｙ | 酵素を含む洗浄組成物 |
| EP3559226B1 (en) | 2016-12-21 | 2023-01-04 | Danisco US Inc. | Bacillus gibsonii-clade serine proteases |
| US20200392477A1 (en) | 2016-12-21 | 2020-12-17 | Danisco Us Inc. | Protease variants and uses thereof |
| IL304833B2 (en) | 2017-02-03 | 2025-11-01 | Codexis Inc | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
| EP3583210B1 (en) | 2017-03-15 | 2021-07-07 | Danisco US Inc. | Trypsin-like serine proteases and uses thereof |
| US20200040283A1 (en) | 2017-03-31 | 2020-02-06 | Danisco Us Inc | Delayed release enzyme formulations for bleach-containing detergents |
| US20200131456A1 (en) | 2017-06-30 | 2020-04-30 | Danisco Us Inc | Low-agglomeration, enzyme-containing particles |
| EP3717643A1 (en) | 2017-11-29 | 2020-10-07 | Danisco US Inc. | Subtilisin variants having improved stability |
| BR112020012584A2 (pt) | 2017-12-21 | 2020-11-24 | Danisco Us Inc. | grânulos fundidos a quente contendo enzima que compreendem um dessecante termotolerante |
| EP3749107A1 (en) | 2018-02-08 | 2020-12-16 | Danisco US Inc. | Thermally-resistant wax matrix particles for enzyme encapsulation |
| WO2019245705A1 (en) | 2018-06-19 | 2019-12-26 | Danisco Us Inc | Subtilisin variants |
| US12415996B2 (en) | 2018-06-19 | 2025-09-16 | Danisco Us Inc. | Subtilisin variants |
| CN121538193A (zh) | 2018-07-30 | 2026-02-17 | 泰莱解决方案美国有限责任公司 | 工程化糖基转移酶和甜菊醇糖苷葡糖基化方法 |
| US20210189295A1 (en) | 2018-08-30 | 2021-06-24 | Danisco Us Inc | Enzyme-containing granules |
| JP2022503923A (ja) | 2018-09-27 | 2022-01-12 | ダニスコ・ユーエス・インク | 医療用器具を洗浄するための組成物 |
| US12509672B2 (en) * | 2018-11-28 | 2025-12-30 | Danisco Us Inc. | Subtilisin variants having improved stability |
| JP7245676B2 (ja) | 2019-03-11 | 2023-03-24 | 花王株式会社 | 変異プロテアーゼ |
| CN114174504A (zh) | 2019-05-24 | 2022-03-11 | 丹尼斯科美国公司 | 枯草杆菌蛋白酶变体和使用方法 |
| WO2020247582A1 (en) | 2019-06-06 | 2020-12-10 | Danisco Us Inc | Methods and compositions for cleaning |
| CN116057158A (zh) | 2020-07-27 | 2023-05-02 | 联合利华知识产权控股有限公司 | 酶和表面活性剂用于抑制微生物的用途 |
| CN116323935A (zh) | 2020-08-27 | 2023-06-23 | 丹尼斯科美国公司 | 用于清洁的酶和酶组合物 |
| EP3981879A1 (en) * | 2020-10-09 | 2022-04-13 | Stada Arzneimittel Ag | Method of producing a recombinant protein in a host cell which has a disabled rhamnose metabolism as well as expression vectors, host cells and recombinant proteins thereof |
| WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
| US20240117275A1 (en) | 2021-01-29 | 2024-04-11 | Danisco Us Inc. | Compositions for cleaning and methods related thereto |
| EP4363565A1 (en) | 2021-06-30 | 2024-05-08 | Danisco US Inc. | Variant lipases and uses thereof |
| EP4396320A2 (en) | 2021-09-03 | 2024-07-10 | Danisco US Inc. | Laundry compositions for cleaning |
| US20240384206A1 (en) | 2021-09-13 | 2024-11-21 | Danisco Us Inc. | Bioactive-containing granules |
| EP4448751A2 (en) | 2021-12-16 | 2024-10-23 | Danisco US Inc. | Subtilisin variants and methods of use |
| CN118715318A (zh) | 2021-12-16 | 2024-09-27 | 丹尼斯科美国公司 | 枯草杆菌蛋白酶变体及其用途 |
| WO2023114939A2 (en) | 2021-12-16 | 2023-06-22 | Danisco Us Inc. | Subtilisin variants and methods of use |
| CN118974227A (zh) | 2022-03-01 | 2024-11-15 | 丹尼斯科美国公司 | 用于清洁的酶和酶组合物 |
| CN119487167A (zh) | 2022-06-21 | 2025-02-18 | 丹尼斯科美国公司 | 用于清洁的包含具有嗜热菌蛋白酶活性的多肽的组合物和方法 |
| WO2024050339A1 (en) | 2022-09-02 | 2024-03-07 | Danisco Us Inc. | Mannanase variants and methods of use |
| CA3265718A1 (en) | 2022-09-02 | 2024-03-07 | Danisco Us Inc. | VARIANTS OF SUBTILISINE AND ASSOCIATED PROCESSES |
| EP4581119A1 (en) | 2022-09-02 | 2025-07-09 | Danisco US Inc. | Detergent compositions and methods related thereto |
| CN120303400A (zh) | 2022-11-09 | 2025-07-11 | 丹尼斯科美国公司 | 枯草杆菌蛋白酶变体和使用方法 |
| EP4658776A1 (en) | 2023-02-01 | 2025-12-10 | Danisco US Inc. | Subtilisin variants and methods of use |
| CN120712348A (zh) | 2023-03-06 | 2025-09-26 | 丹尼斯科美国公司 | 枯草杆菌蛋白酶变体和使用方法 |
| CN120813242A (zh) | 2023-03-16 | 2025-10-17 | 营养与生物科学美国第四公司 | 用于清洁和臭味控制的短芽孢杆菌发酵物提取物及其用途 |
| WO2025071996A1 (en) | 2023-09-28 | 2025-04-03 | Danisco Us Inc. | Variant cutinase enzymes with improved solubility and uses thereof |
| WO2025085351A1 (en) | 2023-10-20 | 2025-04-24 | Danisco Us Inc. | Subtilisin variants and methods of use |
| WO2026024921A1 (en) | 2024-07-25 | 2026-01-29 | The Procter & Gamble Company | Detergent composition comprising a subtilisin variant and methods of use |
| WO2026050315A1 (en) | 2024-08-29 | 2026-03-05 | Danisco Us Inc. | Subtilisin variants and methods of use |
Family Cites Families (103)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1296839A (ru) | 1969-05-29 | 1972-11-22 | ||
| GB1372034A (en) | 1970-12-31 | 1974-10-30 | Unilever Ltd | Detergent compositions |
| GB2048606B (en) | 1979-02-28 | 1983-03-16 | Barr & Stroud Ltd | Optical scanning system |
| DK187280A (da) | 1980-04-30 | 1981-10-31 | Novo Industri As | Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode |
| GR76237B (ru) | 1981-08-08 | 1984-08-04 | Procter & Gamble | |
| US4760025A (en) | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
| US5763257A (en) | 1984-05-29 | 1998-06-09 | Genencor International, Inc. | Modified subtilisins having amino acid alterations |
| US5972682A (en) | 1984-05-29 | 1999-10-26 | Genencor International, Inc. | Enzymatically active modified subtilisins |
| DK171161B1 (da) | 1985-03-28 | 1996-07-08 | Hoffmann La Roche | Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| DK154572C (da) | 1985-08-07 | 1989-04-24 | Novo Industri As | Enzymatisk detergentadditiv, detergent og fremgangsmaade til vask af tekstiler |
| US4933287A (en) | 1985-08-09 | 1990-06-12 | Gist-Brocades N.V. | Novel lipolytic enzymes and their use in detergent compositions |
| US5024943A (en) | 1985-11-07 | 1991-06-18 | Gist-Brocades | Regulatory region cloning and analysis plasmid for bacillus |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| EP0258068B1 (en) | 1986-08-29 | 1994-08-31 | Novo Nordisk A/S | Enzymatic detergent additive |
| GB8629837D0 (en) | 1986-12-13 | 1987-01-21 | Interox Chemicals Ltd | Bleach activation |
| WO1988009367A1 (en) | 1987-05-29 | 1988-12-01 | Genencor, Inc. | Cutinase cleaning composition |
| EP0305216B1 (en) | 1987-08-28 | 1995-08-02 | Novo Nordisk A/S | Recombinant Humicola lipase and process for the production of recombinant humicola lipases |
| JPS6474992A (en) | 1987-09-16 | 1989-03-20 | Fuji Oil Co Ltd | Dna sequence, plasmid and production of lipase |
| EP0394352B1 (en) | 1988-01-07 | 1992-03-11 | Novo Nordisk A/S | Enzymatic detergent |
| JP3079276B2 (ja) | 1988-02-28 | 2000-08-21 | 天野製薬株式会社 | 組換え体ｄｎａ、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法 |
| US4977252A (en) | 1988-03-11 | 1990-12-11 | National Starch And Chemical Investment Holding Corporation | Modified starch emulsifier characterized by shelf stability |
| DE68923398T2 (de) | 1988-05-12 | 1996-01-25 | Procter & Gamble | Flüssige Universalwaschmittel, welche anionische und nichtionische oberflächenaktive Mittel, Gerüststoffe und proteolytisches Enzym enthalten. |
| WO1990009446A1 (en) | 1989-02-17 | 1990-08-23 | Plant Genetic Systems N.V. | Cutinase |
| DK316989D0 (da) | 1989-06-26 | 1989-06-26 | Novo Nordisk As | Enzymer |
| US6271012B1 (en) | 1989-10-11 | 2001-08-07 | Genencor International, Inc. | Protease muteins and their use in detergents |
| EP0528828B2 (de) | 1990-04-14 | 1997-12-03 | Genencor International GmbH | Alkalische bacillus-lipasen, hierfür codierende dna-sequenzen sowie bacilli, die diese lipasen produzieren |
| US5354559A (en) | 1990-05-29 | 1994-10-11 | Grain Processing Corporation | Encapsulation with starch hydrolyzate acid esters |
| EP0495257B1 (en) | 1991-01-16 | 2002-06-12 | The Procter & Gamble Company | Compact detergent compositions with high activity cellulase |
| GB9108136D0 (en) | 1991-04-17 | 1991-06-05 | Unilever Plc | Concentrated detergent powder compositions |
| US5340735A (en) | 1991-05-29 | 1994-08-23 | Cognis, Inc. | Bacillus lentus alkaline protease variants with increased stability |
| BR9307576A (pt) | 1992-12-01 | 1999-06-15 | Novo Nordisk As | Processo para oxidar um substrato com uma enzima peroxidase ou um composto atuando como peroxidase na presença de uma fonte de peróxido de hidrogênio aditivo detergente e composição detergente |
| WO1994016048A1 (en) | 1993-01-18 | 1994-07-21 | The Procter & Gamble Company | Machine dishwashing detergent compositions |
| JPH08509778A (ja) | 1993-05-08 | 1996-10-15 | ヘンケル・コマンディットゲゼルシャフト・アウフ・アクチェン | 銀腐食保護剤（▲ｉｉ▼） |
| CZ286401B6 (en) | 1993-05-08 | 2000-04-12 | Henkel Kgaa | Use of inorganic redox-active substances |
| DK77393D0 (da) | 1993-06-29 | 1993-06-29 | Novo Nordisk As | Aktivering af enzymer |
| US5698504A (en) | 1993-07-01 | 1997-12-16 | The Procter & Gamble Company | Machine dishwashing composition containing oxygen bleach and paraffin oil and benzotriazole compound silver tarnishing inhibitors |
| US5486303A (en) | 1993-08-27 | 1996-01-23 | The Procter & Gamble Company | Process for making high density detergent agglomerates using an anhydrous powder additive |
| CN1057123C (zh) * | 1993-09-15 | 2000-10-04 | 普罗格特-甘布尔公司 | 降低了吸附能力以及增加了水解能力的枯草杆菌蛋白酶bpn变体 |
| MA23346A1 (fr) * | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
| DE4342680A1 (de) | 1993-12-15 | 1995-06-22 | Pfeiffer Erich Gmbh & Co Kg | Austragvorrichtung für Medien |
| US5861271A (en) | 1993-12-17 | 1999-01-19 | Fowler; Timothy | Cellulase enzymes and systems for their expressions |
| ATE512227T1 (de) | 1994-02-24 | 2011-06-15 | Henkel Ag & Co Kgaa | Verbesserte enzyme und detergentien damit |
| US5691295A (en) | 1995-01-17 | 1997-11-25 | Cognis Gesellschaft Fuer Biotechnologie Mbh | Detergent compositions |
| ES2364776T3 (es) | 1994-02-24 | 2011-09-14 | HENKEL AG & CO. KGAA | Enzimas mejoradas y detergentes que las contienen. |
| US5686014A (en) | 1994-04-07 | 1997-11-11 | The Procter & Gamble Company | Bleach compositions comprising manganese-containing bleach catalysts |
| US6599730B1 (en) * | 1994-05-02 | 2003-07-29 | Procter & Gamble Company | Subtilisin 309 variants having decreased adsorption and increased hydrolysis |
| EP0766727B1 (en) | 1994-06-17 | 2002-08-14 | Genencor International, Inc. | Cleaning method based on compositions containing a hemicellulase plant cell wall degrading enzyme and the use thereof in cleaning methods |
| GB2294268A (en) | 1994-07-07 | 1996-04-24 | Procter & Gamble | Bleaching composition for dishwasher use |
| US5879584A (en) | 1994-09-10 | 1999-03-09 | The Procter & Gamble Company | Process for manufacturing aqueous compositions comprising peracids |
| US5516448A (en) | 1994-09-20 | 1996-05-14 | The Procter & Gamble Company | Process for making a high density detergent composition which includes selected recycle streams for improved agglomerate |
| US5691297A (en) | 1994-09-20 | 1997-11-25 | The Procter & Gamble Company | Process for making a high density detergent composition by controlling agglomeration within a dispersion index |
| US5489392A (en) | 1994-09-20 | 1996-02-06 | The Procter & Gamble Company | Process for making a high density detergent composition in a single mixer/densifier with selected recycle streams for improved agglomerate properties |
| EP0796317B1 (en) | 1994-12-09 | 2000-03-01 | The Procter & Gamble Company | Automatic dishwashing composition containing particles of diacyl peroxides |
| US5534179A (en) | 1995-02-03 | 1996-07-09 | Procter & Gamble | Detergent compositions comprising multiperacid-forming bleach activators |
| US5574005A (en) | 1995-03-07 | 1996-11-12 | The Procter & Gamble Company | Process for producing detergent agglomerates from high active surfactant pastes having non-linear viscoelastic properties |
| US5569645A (en) | 1995-04-24 | 1996-10-29 | The Procter & Gamble Company | Low dosage detergent composition containing optimum proportions of agglomerates and spray dried granules for improved flow properties |
| EP0827531A2 (en) * | 1995-05-05 | 1998-03-11 | Unilever N.V. | Subtilisin variants |
| US5597936A (en) | 1995-06-16 | 1997-01-28 | The Procter & Gamble Company | Method for manufacturing cobalt catalysts |
| JP4592832B2 (ja) | 1995-06-16 | 2010-12-08 | ザ プロクター アンド ギャンブル カンパニー | コバルト触媒を含む自動皿洗い組成物 |
| US5565422A (en) | 1995-06-23 | 1996-10-15 | The Procter & Gamble Company | Process for preparing a free-flowing particulate detergent composition having improved solubility |
| DE19530816A1 (de) | 1995-08-23 | 1997-02-27 | Cognis Bio Umwelt | Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten |
| US5576282A (en) | 1995-09-11 | 1996-11-19 | The Procter & Gamble Company | Color-safe bleach boosters, compositions and laundry methods employing same |
| ES2174105T5 (es) | 1995-09-18 | 2007-03-01 | THE PROCTER & GAMBLE COMPANY | Sistemas de liberacion. |
| MA24136A1 (fr) | 1996-04-16 | 1997-12-31 | Procter & Gamble | Fabrication d'agents de surface . |
| BR9812093B1 (pt) | 1997-03-07 | 2009-12-01 | composição detergente alvejante. | |
| AU6226198A (en) | 1997-03-07 | 1998-09-22 | Procter & Gamble Company, The | Improved methods of making cross-bridged macropolycycles |
| DK0985042T3 (da) * | 1997-06-04 | 2007-01-15 | Genencor Int | Proteaseenzymer til vanskelig rengöring og/eller plet- og filmreduktion samt præparater, der inkorporerer disse |
| GB2327947A (en) | 1997-08-02 | 1999-02-10 | Procter & Gamble | Detergent tablet |
| DE19736591A1 (de) | 1997-08-22 | 1999-02-25 | Peter Prof Dr Hegemann | Verfahren zum Herstellen von Nukleinsäurepolymeren |
| JP5095884B2 (ja) * | 1997-08-29 | 2012-12-12 | ノボザイムス アクティーゼルスカブ | プロテアーゼ変異体及び組成物 |
| RU2269572C2 (ru) * | 1997-10-23 | 2006-02-10 | Джененкор Интернэшнл, Инк. | Варианты протеазы, замещенные в нескольких положениях |
| AR015977A1 (es) | 1997-10-23 | 2001-05-30 | Genencor Int | Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes |
| US5935826A (en) | 1997-10-31 | 1999-08-10 | National Starch And Chemical Investment Holding Corporation | Glucoamylase converted starch derivatives and their use as emulsifying and encapsulating agents |
| US6773907B2 (en) | 1997-11-21 | 2004-08-10 | Peter Kamp Hansen | Subtilase enzymes |
| CA2310454C (en) | 1997-11-21 | 2012-01-24 | Novo Nordisk A/S | Protease variants and compositions |
| AU2207099A (en) | 1997-12-24 | 1999-07-19 | Genencor International, Inc. | An improved method of assaying for a preferred enzyme and/or preferred detergentcomposition |
| US6835550B1 (en) | 1998-04-15 | 2004-12-28 | Genencor International, Inc. | Mutant proteins having lower allergenic response in humans and methods for constructing, identifying and producing such proteins |
| BRPI9911086B1 (pt) | 1998-06-10 | 2016-08-02 | Novozymes As | composição de limpeza, processo para tratar tecidos a máquina, e uso de uma mananase |
| US6376450B1 (en) | 1998-10-23 | 2002-04-23 | Chanchal Kumar Ghosh | Cleaning compositions containing multiply-substituted protease variants |
| WO2000032601A2 (en) | 1998-11-30 | 2000-06-08 | The Procter & Gamble Company | Process for preparing cross-bridged tetraaza macrocycles |
| CA2374350C (en) | 1999-05-20 | 2012-10-23 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 97 and 98 |
| MXPA02000840A (es) | 1999-07-22 | 2002-07-30 | Procter & Gamble | Variantes de proteasa de subtilisina que tienen substituciones de aminoacidos en regiones de epitopes definidas. |
| JP2003505069A (ja) | 1999-07-22 | 2003-02-12 | ザ、プロクター、エンド、ギャンブル、カンパニー | 立体的に保護されたエピトープ領域を有するプロテアーゼ抱合体 |
| US6946128B1 (en) | 1999-07-22 | 2005-09-20 | The Procter & Gamble Company | Protease conjugates having sterically protected epitope regions |
| JP2003505067A (ja) * | 1999-07-22 | 2003-02-12 | ザ、プロクター、エンド、ギャンブル、カンパニー | 特定のエピトープ領域にアミノ酸欠失及び置換を有するサブチリシンプロテアーゼの変異体 |
| ATE400648T1 (de) | 2000-08-11 | 2008-07-15 | Genencor Int | Transformation von bacillus, transformanten und mutanten-bibliotheken |
| US6440991B1 (en) | 2000-10-02 | 2002-08-27 | Wyeth | Ethers of 7-desmethlrapamycin |
| US6582914B1 (en) | 2000-10-26 | 2003-06-24 | Genencor International, Inc. | Method for generating a library of oligonucleotides comprising a controlled distribution of mutations |
| DK200101090A (da) * | 2001-07-12 | 2001-08-16 | Novozymes As | Subtilase variants |
| DK1523553T3 (da) | 2002-01-16 | 2010-04-19 | Genencor Int | Multi-substituerede proteasevarianter |
| DE10260805A1 (de) | 2002-12-23 | 2004-07-22 | Geneart Gmbh | Verfahren und Vorrichtung zum Optimieren einer Nucleotidsequenz zur Expression eines Proteins |
| CN103333870A (zh) | 2003-12-03 | 2013-10-02 | 丹尼斯科美国公司 | 过水解酶 |
| CN105200027B (zh) | 2005-10-12 | 2019-05-31 | 金克克国际有限公司 | 储存稳定的中性金属蛋白酶的用途和制备 |
| US20080004201A1 (en) | 2006-06-05 | 2008-01-03 | Jean-Pol Boutique | Enzyme stabilizer |
| KR20150115023A (ko) | 2006-07-18 | 2015-10-13 | 다니스코 유에스 인크. | 광범위한 온도에 걸쳐 활성인 프로테아제 변이형 |
| EP2100947A1 (en) | 2008-03-14 | 2009-09-16 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| EP2297314B1 (en) | 2008-06-06 | 2015-04-15 | Danisco US Inc. | Compositions and methods comprising variant microbial proteases |
| EP3031894A1 (en) | 2008-11-11 | 2016-06-15 | Danisco US Inc. | Proteases comprising one or more combinable mutations |
| EP2510092A1 (en) | 2009-12-09 | 2012-10-17 | The Procter & Gamble Company | Fabric and home care products |
| US20120067373A1 (en) | 2010-04-15 | 2012-03-22 | Philip Frank Souter | Automatic Dishwashing Detergent Composition |
-
2009
- 2009-11-10 EP EP15194914.6A patent/EP3031894A1/en not_active Withdrawn
- 2009-11-10 JP JP2011536413A patent/JP2012508031A/ja active Pending
- 2009-11-10 BR BR122013014157-0A patent/BR122013014157A2/pt not_active Application Discontinuation
- 2009-11-10 US US13/128,854 patent/US8753861B2/en active Active
- 2009-11-10 MX MX2011004802A patent/MX2011004802A/es active IP Right Grant
- 2009-11-10 EP EP20090752658 patent/EP2362896A2/en not_active Withdrawn
- 2009-11-10 BR BRPI0922083A patent/BRPI0922083A2/pt not_active Application Discontinuation
- 2009-11-10 CN CN2009801445264A patent/CN102209775A/zh active Pending
- 2009-11-10 CN CN201610814735.7A patent/CN106399280B/zh active Active
- 2009-11-10 CN CN201310225070.2A patent/CN103361196B/zh not_active Expired - Fee Related
- 2009-11-10 AR ARP090104342A patent/AR074311A1/es unknown
- 2009-11-10 RU RU2011123739/10A patent/RU2560978C2/ru not_active IP Right Cessation
- 2009-11-10 KR KR1020117010706A patent/KR20110095260A/ko not_active Ceased
- 2009-11-10 CN CN201310031350XA patent/CN103122345A/zh active Pending
- 2009-11-10 EP EP13171133.5A patent/EP2650354A3/en not_active Withdrawn
- 2009-11-10 WO PCT/US2009/063837 patent/WO2010056653A2/en not_active Ceased
- 2009-11-10 CA CA2743302A patent/CA2743302A1/en not_active Abandoned
-
2013
- 2013-06-07 JP JP2013120314A patent/JP5893584B2/ja not_active Expired - Fee Related
-
2014
- 2014-05-02 US US14/268,488 patent/US9096838B2/en not_active Expired - Fee Related
- 2014-08-07 JP JP2014161183A patent/JP6120815B2/ja active Active
- 2014-11-10 US US14/537,660 patent/US20150065412A1/en not_active Abandoned
-
2015
- 2015-06-29 US US14/754,355 patent/US20160010072A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20150065412A1 (en) | 2015-03-05 |
| EP2650354A3 (en) | 2014-02-12 |
| RU2560978C2 (ru) | 2015-08-20 |
| BRPI0922083A2 (pt) | 2017-05-30 |
| EP2362896A2 (en) | 2011-09-07 |
| JP2015015952A (ja) | 2015-01-29 |
| CN102209775A (zh) | 2011-10-05 |
| CN103122345A (zh) | 2013-05-29 |
| US20120003718A1 (en) | 2012-01-05 |
| AR074311A1 (es) | 2011-01-05 |
| US20140308736A1 (en) | 2014-10-16 |
| CA2743302A1 (en) | 2010-05-20 |
| CN106399280B (zh) | 2020-12-22 |
| EP3031894A1 (en) | 2016-06-15 |
| CN103361196B (zh) | 2016-01-27 |
| US20160010072A1 (en) | 2016-01-14 |
| JP2012508031A (ja) | 2012-04-05 |
| BR122013014157A2 (pt) | 2018-11-13 |
| US9096838B2 (en) | 2015-08-04 |
| KR20110095260A (ko) | 2011-08-24 |
| US8753861B2 (en) | 2014-06-17 |
| CN103361196A (zh) | 2013-10-23 |
| CN106399280A (zh) | 2017-02-15 |
| JP6120815B2 (ja) | 2017-04-26 |
| JP2013236632A (ja) | 2013-11-28 |
| EP2650354A2 (en) | 2013-10-16 |
| WO2010056653A3 (en) | 2010-09-16 |
| JP5893584B2 (ja) | 2016-03-23 |
| MX2011004802A (es) | 2011-06-16 |
| WO2010056653A2 (en) | 2010-05-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2011123739A (ru) | Протеазы, содержащие одну или несколько комбинируемых мутаций | |
| RU2010153866A (ru) | Композиции и способы, включающие в себя применение вариантных микробных протеаз | |
| RU2011123745A (ru) | Композиции и способы, включающие вариант субтилизина | |
| Zhou et al. | A novel alkaline protease from alkaliphilic Idiomarina sp. C9-1 with potential application for eco-friendly enzymatic dehairing in the leather industry | |
| RU2014140895A (ru) | Продуцирование термолизина и его вариантов и его использование в жидких детергентах | |
| RU2013147763A (ru) | Способы и композиции, содержащие варианты сериновой протеазы | |
| JP2014522235A5 (ru) | ||
| RU2013153802A (ru) | Композиции и способы, включающие варианты сериновой протеазы | |
| US8329632B2 (en) | Detergent compositions and the use of enzyme combinations therein | |
| JP2016538860A5 (ru) | ||
| FI102907B (fi) | Mutatoitu subtilisiini-proteaasi | |
| US7183248B2 (en) | Enzymatic cleaner having high pH stability | |
| RU2617406C2 (ru) | Стабильное при хранении жидкое моющее или чистящее средство, содержащее протеазу и амилазу | |
| RU2015117648A (ru) | Композиции и способы, предусматривающие вариант липолитического фермента | |
| EP3575389A3 (en) | Consumer products with protease variants | |
| RU2017143361A (ru) | Варианты протеазы клады aprl и их применения | |
| EP0405901A1 (en) | Enzymatic detergent compositions | |
| RU2013132813A (ru) | Стабильное при хранении жидкое моющее или чистящее средство, содержащее протеазу и амилазу | |
| RU2009118608A (ru) | Варианты сериновой протеазы с множественными мутациями | |
| RU2013132812A (ru) | Стабильное при хранении жидкое моющее или чистящее средство, содержащее протеазу и целлюлазу | |
| RU2010130255A (ru) | Ферментативное предотвращение образования и контроль биопленки | |
| RU2014135160A (ru) | Стабильное при хранении жидкое средство для мытья посуды, содержащее протеазу и амилазу | |
| RU2010122901A (ru) | Варианты альфа-амилазы с измененными свойствами | |
| RU2011123911A (ru) | Композиции, содержащие варианты сериновых протеаз, и способы | |
| JP2013527767A5 (ru) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20161111 |