RU2005105321A - Новый фермент, образующий пептид, микроорганизм, продуцирующий данный фермент, и способ синтеза дипептида с их применением - Google Patents

Новый фермент, образующий пептид, микроорганизм, продуцирующий данный фермент, и способ синтеза дипептида с их применением Download PDF

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RU2005105321A
RU2005105321A RU2005105321/13A RU2005105321A RU2005105321A RU 2005105321 A RU2005105321 A RU 2005105321A RU 2005105321/13 A RU2005105321/13 A RU 2005105321/13A RU 2005105321 A RU2005105321 A RU 2005105321A RU 2005105321 A RU2005105321 A RU 2005105321A
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enzyme
component
peptide
ability
dipeptide
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RU2005105321/13A
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RU2300565C2 (ru
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Кензо ЕКОЗЕКИ (JP)
Кензо ЕКОЗЕКИ
Соноко СУЗУКИ (JP)
Соноко СУЗУКИ
Сейити ХАРА (JP)
Сейити ХАРА
Сатоси КАТАЯМА (JP)
Сатоси КАТАЯМА
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Адзиномото Ко., Инк. (Jp)
Адзиномото Ко., Инк.
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    • C07K5/06—Dipeptides
    • C07K5/06008—Dipeptides with the first amino acid being neutral
    • C07K5/06017—Dipeptides with the first amino acid being neutral and aliphatic
    • C07K5/06026—Dipeptides with the first amino acid being neutral and aliphatic the side chain containing 0 or 1 carbon atom, i.e. Gly or Ala
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
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    • C07K5/06017—Dipeptides with the first amino acid being neutral and aliphatic
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    • C07K5/06069—Ser-amino acid
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    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
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    • C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
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    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1005—Tetrapeptides with the first amino acid being neutral and aliphatic
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Claims (11)

1. Фермент, полученный из микроорганизма, относящегося к роду, выбранному из рода Empedobacter и рода Sphingobacterium, и обладающий способностью образовывать пептид из карбоксильного компонента и аминного компонента.
2. Фермент, обладающий способностью образовывать пептид из карбоксильного компонента и аминного компонента и способностью образовывать L-аланил-L-глутамин со скоростью образования 0,03 мМ/мин или выше в реакции образования дипептида при условиях (i)-(iv):
(i) карбоксильным компонентом является гидрохлорид сложного метилового эфира L-аланина в количестве 100 мМ;
(ii) аминным компонентом является L-глутамин в количестве 200 мМ;
(iii) pH равно 9,0; и
(iv) количество добавляемого гомогенно очищенного фермента составляет менее 0,61 мг/мл в расчете на белок.
3. Фермент по п.1 или 2, в котором карбоксильный компонент в качестве субстрата включает как сложный эфир аминокислоты, так и амид аминокислоты.
4. Фермент по п.1 или 2, в котором аминный компонент в качестве субстрата включает любое из аминокислоты, C-защищенной аминокислоты и амина.
5. Фермент по п.1 или 2, в котором фермент обладает способностью образовывать пептид в диапазоне pH от 6,5 до 10,5.
6. Фермент по п.1 или 2, в котором фермент обладает способностью образовывать пептид в диапазоне температур от 0 до 60°C.
7. Фермент по п.1 или 2, в котором фермент не ингибируется ингибитором серинового фермента, фенилметилсульфонилфторидом, но ингибируется пара-нитрофенил-пара'-гуанидинобензоатом.
8. Фермент по п.1 или 2, в котором фермент имеет молекулярную массу, определенную посредством SDS-гель-электрофореза, составляющую примерно 75 килодальтон, и молекулярную массу, определенную посредством хроматографии на основе гель-фильтрации, составляющую примерно 150 килодальтон.
9. Микроорганизм, который продуцирует фермент по п.1 или 2.
10. Микроорганизм по п.9, в котором микроорганизм выбран из Empedobacter brevis, штамм FERM BP-8113 и Sphingobacterium sp., штамм FERM BP-8124.
11. Способ получения дипептида, включающий в себя образование дипептида из карбоксильного компонента и аминного компонента с использованием фермента по п.1 или 2 или вещества, содержащего фермент.
RU2005105321/13A 2002-07-26 2003-07-25 Новый фермент, образующий пептид, микроорганизм, продуцирующий данный фермент, и способ синтеза дипептида с их применением RU2300565C2 (ru)

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JP2002218956 2002-07-26
JP2002-218956 2002-07-26

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RU2005105321A true RU2005105321A (ru) 2005-08-10
RU2300565C2 RU2300565C2 (ru) 2007-06-10

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US (1) US20040253665A1 (ru)
EP (1) EP1553171A4 (ru)
JP (1) JP4501689B2 (ru)
KR (1) KR20050025677A (ru)
CN (1) CN1316016C (ru)
AU (1) AU2003248117A1 (ru)
BR (1) BR0305638A (ru)
CA (1) CA2509765C (ru)
PL (2) PL211730B1 (ru)
RU (1) RU2300565C2 (ru)
WO (1) WO2004022733A1 (ru)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005168405A (ja) 2003-12-11 2005-06-30 Ajinomoto Co Inc ジペプチドの製造方法
CN101087878A (zh) * 2004-12-20 2007-12-12 味之素株式会社 具有肽合成活性的突变型蛋白质
WO2006104186A1 (ja) * 2005-03-29 2006-10-05 Kyowa Hakko Kogyo Co., Ltd. ジペプチドの結晶およびその製造法
EP2036919A4 (en) * 2006-06-28 2012-01-11 Kyowa Hakko Bio Co Ltd PROCESS FOR PURIFYING AN OLIGOPEPTIDE
JPWO2009139392A1 (ja) * 2008-05-12 2011-09-22 味の素株式会社 β−アラニルアミノ酸またはその誘導体の製造方法
DE102009002044A1 (de) * 2009-03-31 2010-10-07 Evonik Degussa Gmbh Dipeptide als Futtermitteladditive
CN104561202B (zh) * 2015-02-06 2018-02-09 江苏诚信药业有限公司 一种酶催化合成丙谷二肽的制备方法及工艺系统
CA3049488C (en) 2016-12-30 2020-07-28 Innobio Corporation Limited Gene encoding alanyl-glutamine dipeptide biosynthetic enzyme and application thereof
CN106754447B (zh) * 2016-12-30 2020-08-25 大连医诺生物股份有限公司 重组酿酒酵母及其在合成丙谷二肽中的应用
CN106754985B (zh) * 2016-12-30 2019-08-13 大连医诺生物股份有限公司 编码丙谷二肽生物合成酶的基因及其应用
US10793594B2 (en) 2017-04-19 2020-10-06 Indiana University Research And Technology Corporation Antimicrobial compounds and/or modulators of microbial infections and methods of using the same
KR102650468B1 (ko) * 2024-01-19 2024-03-25 큐티스바이오 주식회사 생물전환공정을 이용한 디펩타이드의 제조방법

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2953499A (en) * 1956-04-19 1960-09-20 Ajimomoto Co Inc Process for producing l-glutamic acid from hardly soluble amino-acid
US3847744A (en) * 1971-12-14 1974-11-12 Ajinomoto Kk Method of producing elastases by bacteria
DK72587D0 (da) * 1987-02-13 1987-02-13 Carlsberg Biotechnology Ltd Fremgangsmaade til enzymatisk fremstilling af dipeptider
CA1337936C (en) * 1987-08-07 1996-01-16 Akiva Tuvia Gross Vibriolysin coupling process
DK163435C (da) * 1988-08-12 1992-07-20 Carlsberg Biotechnology Ltd Fremgangsmaade til enzymatisk fremstilling af dipeptider og derivater deraf
JP4613282B2 (ja) * 1999-04-12 2011-01-12 アークレイ株式会社 α−糖化アミノ酸遊離酵素

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JPWO2004022733A1 (ja) 2005-12-22
US20040253665A1 (en) 2004-12-16
CN1671840A (zh) 2005-09-21
CN1316016C (zh) 2007-05-16
CA2509765C (en) 2011-04-26
RU2300565C2 (ru) 2007-06-10
EP1553171A4 (en) 2005-09-21
KR20050025677A (ko) 2005-03-14
PL211754B1 (pl) 2012-06-29
PL211730B1 (pl) 2012-06-29
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