RU2003136863A -
PES4-4 RECOMBINANT PLASMID DNA ENCODING THE HUMAN INTERPHERON ALPHA-2B POLYPEPTIDE AND STRAIN ESCHERICHIA COLI BDEES4-PRODUCER OF ALF-2 RECOMBINANT INTERFERON
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PES4-4 RECOMBINANT PLASMID DNA ENCODING THE HUMAN INTERPHERON ALPHA-2B POLYPEPTIDE AND STRAIN ESCHERICHIA COLI BDEES4-PRODUCER OF ALF-2 RECOMBINANT INTERFERON
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RU2003136863A
RU2003136863ARU2003136863/13ARU2003136863ARU2003136863ARU 2003136863 ARU2003136863 ARU 2003136863ARU 2003136863/13 ARU2003136863/13 ARU 2003136863/13ARU 2003136863 ARU2003136863 ARU 2003136863ARU 2003136863 ARU2003136863 ARU 2003136863A
Институт биоорганической химии им. академиков М.М. Шем кина и Ю.А. Овчинникова РАН (RU)
Институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова РАН
Закрытое акционерное общество "Мастерклон" (RU)
Закрытое Акционерное Общество "Мастерклон"
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1. Рекомбинантная плазмидная ДНК pES4-4, кодирующая полипептид с последовательностью интерферона α-2b человека, имеющая молекулярную массу 3,64 Мда (5917 п.о.), состоящая из: NdeI/BamHI-фрагмента ДНК плазмиды рЕТ22b(+), содержащего промотор и терминатор транскрипции Т7-РНК-полимеразы и усилитель трансляции гена 10 фага Т7; NdeI/BamHI-фрагмента ДНК, содержащего последовательность искусственного гена рекомбинантного интерферона α-2b человека, приведенную на фиг.1 и содержащая ген β-лактамазы, детерминирующий устойчивость трансформированных плазмидой pES4-4 клеток Е.coli к ампициллину, в качестве генетического маркера, уникальные сайты узнавания эндонуклеаз рестрикции, расположенные на следующем расстоянии вправо от сайта NdeI: XbaI – 38 п.о., HpaI – 1332 п.о., PstI – 4065 п.о., PvuI – 4190 п.о., XhoI – 5363 п.о.1. Recombinant plasmid DNA pES4-4 encoding a polypeptide with the sequence of human interferon α-2b, having a molecular weight of 3.64 Mda (5917 bp), consisting of: NdeI / BamHI DNA fragment of the plasmid pET22b (+) containing promoter and terminator of transcription of T7 RNA polymerase and translation enhancer of gene 10 of phage T7; The NdeI / BamHI DNA fragment containing the sequence of the artificial recombinant human interferon α-2b gene shown in Fig. 1 and containing the β-lactamase gene that determines the resistance of ampicillin to E. coli transformed with plasmid pES4-4 as unique genetic marker recognition sites of restriction endonucleases located at the following distance to the right of the NdeI site: XbaI - 38 bp, HpaI - 1332 bp, PstI - 4065 bp, PvuI - 4190 bp, XhoI - 5363 p .about.2. Штамм Escherichia coli BDEES4, содержащий рекомбинантную плазмидную ДНК pES4-4, продуцент рекомбинантного интерферона α-2b человека.2. The strain Escherichia coli BDEES4 containing recombinant plasmid DNA pES4-4, producer of recombinant human interferon α-2b.
RU2003136863/13A2003-12-232003-12-23Recombinant plasmid dna pes4-4 encoding human polypeptide interferon alpha-2b and strain escherichia coli brees4 as producer of recombinant human interferon alpha-2b
RU2258081C1
(en)
Recombinant plasmid dna pes4-4 encoding human polypeptide interferon alpha-2b and strain escherichia coli brees4 as producer of recombinant human interferon alpha-2b
Recombinant plasmid dna pes4-4 encoding human polypeptide interferon alpha-2b and strain escherichia coli brees4 as producer of recombinant human interferon alpha-2b
Recombinant plasmid dna pes4-4 encoding human polypeptide interferon alpha-2b and strain escherichia coli brees4 as producer of recombinant human interferon alpha-2b
PES4-4 RECOMBINANT PLASMID DNA ENCODING THE HUMAN INTERPHERON ALPHA-2B POLYPEPTIDE AND STRAIN ESCHERICHIA COLI BDEES4-PRODUCER OF ALF-2 RECOMBINANT INTERFERON
PES6-1 RECOMBINANT PLASMID DNA ENCODING HUMAN INTERFERON BETA-1B POLYPEPTIDE AND STRAIN ESCHERICHIA COLI BDEES6-PRODUCER OF RECOMBINANT BETA-1B INTERFERON
P30NE2 RECOMBINANT PLASMID DNA ENCODING THE 181-AMINO ACID N-TERMINAL FRAGMENT OF CLASSIC PIG VIRUS VIRUS E2 GLYCOPROTEIN AND PROVIDING ITS EXCESSLESS E.
Recombinant plasmid DNA pQe30_PS-CFP2 / TurboYFP_MBP7, coding fragment of human myelin basic protein fused 80-104 IN THE COMPOSITION OF STRUCTURES with yellow and cyan fluorescent protein EPeFRET, STRAIN Escherichia coli BL21 (DE3) - PRODUCER said protein, AND METHOD OF PRODUCING RECOMBINANT PROTEIN EPeFRET
RECOMBINANT PLASMID DNA pET-hHSP70, ENCODING THE HEAT SHOCK PROTEIN hSP70, AND THE STRAIN E. coli BL21 (DE3) / pET-hHSP70 - PRODUCT HUMAN SHOCK PROTEIN hSP70
RECOMBINANT PLASMID DNA PLP-3,1, ENCODING HUMAN LYSPRO PROINSULINE POLYPEPTIDE, AND STRAIN OF ESCHERICHIA COLI PLP-3-1 / TG-1 PRODUCER OF RECOMBINANT L PROINSPROIN
Recombinant plasmid DNA p31NC encoding 125 amino acid nucleocapsid protein of the pig reproductive and respiratory syndrome virus and ensuring its expression in E. coli bacteria cells
PAS-2 RECOMBINANT PLASMID DNA ENCODING A HUMAN ASPART PROINSULINE PROLINE POLYPEPTIDE AND ESCHERICHIA COLI BAS2 STRAIN OF BACTERIA - PRODUCER OF ASPART RECOMBINANT PROINSULIN
PTRCTE-LEP RECOMBINANT PLASMID DNA ENCODING A POLYPEPTIDE WITH HUMAN LEPTINE PROPERTIES AND ESCHERICHIA COLI STRAIN BACTERIA - PRODUCER OF A LEPTOPINE PROPERTIES
Recombinant plasmid DNA pET32a-DomIII-E-ZIKV, which provides expression of a gene fragment E encoding domain III of structural Zlyvirus glycoprotein E and recombinant protein Dom III-E-ZIKV, designed to induce specific immunity against Zika virus
Recombinant plasmid DNA pUL83HCMV, which provides expression of the recombinant protein containing the immunodominant part of the phosphoprotein pp65 HCMV and a B-galactosidase fragment in the cells of the bacterium Escherichia coli
Recombinant plasmid DNA PUL44HCMV, which provides for the expression of a fragment of the human cytomegalovirus UL44 gene encoding the immunomodulant part of the Black-chain-free bark-free birch bark
MODULAR RECOMBINANT PLASMID, EXPRESSING A MODULAR POLYPEPTIDE FOR DELIVERY OF A PHOTOSENSIBILIZER, AND STRAIN ESCHERICHIA COLI VKPM B-8356 - PRODUCER OF A MODULAR POLYPEPT
Recombinant plasmid DNA PHSVD1, which provides for the expression of a fragment of the US6 gene of the herpes simplex virus type 1, which encodes a glycolechoid geropolytic syndrome
POLYPEPTIDE E 495 for determining antibodies to T-cell leukemia I TYPE (HTLV-I), DNA fragments ATLE 495 encoding the polypeptide E 495, with recombinant plasmid DNA PATLE 495 encoding the polypeptide E 495, STRAIN BACTERIA ESCHERICHIA COLI - PRODUCER POLYPEPTIDE E 495.
Recombinant plasmid DNA PFASTBAC-G2R, containing units of variola virus genomes protein coding ANALOG TUMOR NECROSIS FACTOR RECEPTOR AND STRAIN baculovirus BTRI67, producing SOLUBLE TUMOR NECROSIS FACTOR RECEPTOR VARIOLA VIRUS