RU2002131715A -
PTRCTE-OPH RECOMBINANT PLASMID DNA AND ORGANOPHOSPHATHYDROLASE ENZYMES PRODUCER
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PTRCTE-OPH RECOMBINANT PLASMID DNA AND ORGANOPHOSPHATHYDROLASE ENZYMES PRODUCER
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Publication number
RU2002131715A
RU2002131715ARU2002131715/13ARU2002131715ARU2002131715ARU 2002131715 ARU2002131715 ARU 2002131715ARU 2002131715/13 ARU2002131715/13 ARU 2002131715/13ARU 2002131715 ARU2002131715 ARU 2002131715ARU 2002131715 ARU2002131715 ARU 2002131715A
1. Рекомбинантная плазмидная ДНК pTгсТЕ-ОРН, кодирующая полипептид со свойствами органофосфатгидролазы с молекулярной массой 3,46 Md (5,243 т.п.о.), включающая Cla I/Hind III - фрагмент ДНК плазмиды pTrcTEGF длиной 4,232 т.п.о., trc-промотор Е. coli, синтетический усилитель трансляции TREN гена 10 бактериофага Т7, ген bla β-лактамазы, определяющий устойчивость трансформированных плазмидой pTrсТЕ-ОРН клеток к ампициллину, участок ori инициации репликации, ДНК длиной 1,011 т.п.о, кодирующую аминокислотную последовательность зрелой формы органофосфатгидролазы, фланкированную сайтами рестрикции Cla I и Hind III, и содержащая уникальные сайты узнавания рестрикционными эндонуклеазами со следующими координатами: Nco I-265, Eco RI-270, Kpn I-286, Xba I-340, Cla I-377, Hind III-1389.1. Recombinant plasmid DNA pTcTE-ORN encoding a polypeptide with the properties of organophosphate hydrolase with a molecular weight of 3.46 Md (5.243 kb), including Cla I / Hind III - a DNA fragment of the plasmid pTrcTEGF 4.232 kb in length. , trc promoter E. coli, synthetic translation enhancer of TREN gene 10 of bacteriophage T7 gene, bla β-lactamase gene, which determines the resistance of ampicillin to plasmid pTrcTE-ORH plasmid, replication initiation ori site, 1.011 kb DNA encoding amino acid the sequence of the mature form of organophosphate hydrolase flanked with restriction sites Cla I and Hind III, and containing unique recognition sites by restriction endonucleases with the following coordinates: Nco I-265, Eco RI-270, Kpn I-286, Xba I-340, Cla I-377, Hind III-1389.2. Штамм бактерий Escherichia coli DH5α/pTrcTE-OPH - продуцент полипептида со свойствами органофосфатгидролазы.2. The bacterial strain Escherichia coli DH5α / pTrcTE-OPH is a producer of a polypeptide with the properties of organophosphate hydrolase.
RU2002131715/13A2002-11-262002-11-26Recombinant plasmid dna ptrcte-oph and producer of enzyme organophosphate hydrolase
RU2232807C1
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