RU2004100070A - MODULAR RECOMBINANT PLASMID, EXPRESSING A MODULAR POLYPEPTIDE FOR DELIVERY OF A PHOTOSENSIBILIZER, AND STRAIN ESCHERICHIA COLI VKPM B-8356 - PRODUCER OF A MODULAR POLYPEPT - Google Patents

MODULAR RECOMBINANT PLASMID, EXPRESSING A MODULAR POLYPEPTIDE FOR DELIVERY OF A PHOTOSENSIBILIZER, AND STRAIN ESCHERICHIA COLI VKPM B-8356 - PRODUCER OF A MODULAR POLYPEPT Download PDF

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Publication number
RU2004100070A
RU2004100070A RU2004100070/13A RU2004100070A RU2004100070A RU 2004100070 A RU2004100070 A RU 2004100070A RU 2004100070/13 A RU2004100070/13 A RU 2004100070/13A RU 2004100070 A RU2004100070 A RU 2004100070A RU 2004100070 A RU2004100070 A RU 2004100070A
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RU
Russia
Prior art keywords
modular
producer
photosensibilizer
polypept
expressing
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RU2004100070/13A
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Russian (ru)
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RU2265055C2 (en
Inventor
Владимир Глебович Лунин (RU)
Владимир Глебович Лунин
Ольга Васильевна Сергиенко (RU)
Ольга Васильевна Сергиенко
Ольга Львовна Воронина (RU)
Ольга Львовна Воронина
занова Елена Михайловна Р (RU)
Елена Михайловна Рязанова
Андрей Александрович Розенкранц (RU)
Андрей Александрович Розенкранц
Александр Сергеевич Соболев (RU)
Александр Сергеевич Соболев
Original Assignee
Закрытое акционерное общество "АСГЛ-Исследовательские лаборатории" (RU)
Закрытое акционерное общество "АСГЛ-Исследовательские Лаборатории"
Александр Сергеевич Соболев (RU)
Александр Сергеевич Соболев
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Priority to RU2004100070/13A priority Critical patent/RU2265055C2/en
Publication of RU2004100070A publication Critical patent/RU2004100070A/en
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Publication of RU2265055C2 publication Critical patent/RU2265055C2/en

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (3)

1. Модульный рекомбинантный полипептид в качестве носителя фотосенсибилизаторов, длиной 694, М. м. 76306 Д, имеющий следующую структуру: 6His-HMP-NLS-DTox-(Gly-Ser)5-EGF, в котором 6His - аа 5-10, НМР - аа 14-409, DTox -аа 439-624, 198-384 с заменами, (Gly-Ser)5-аа 628-638, EGF - аа 642-694. (seq id №1).1. A modular recombinant polypeptide as a carrier of photosensitizers, length 694, M. m. 76306 D, having the following structure: 6His-HMP-NLS-DTox- (Gly-Ser) 5 -EGF, in which 6His - aa 5-10, NMR - aa 14-409, DTox - aa 439-624, 198-384 with replacements, (Gly-Ser) 5 -aa 628-638, EGF - aa 642-694. (seq id No. 1). 2. Плазмида pR752 размером 5269 оснований (SEQ ID №2), содержащая следующие регуляторные области и кодирующие участки: искусственный бактериальный оперон, содержащий промоторную область бактериофага Т5 и ген, кодирующий мультидоменный белок 6His-HMP-NLS-DTox-(Gly-Ser)5-EGF (кодирующая область - 115-2199 н.п.); бактериальный оперон bla, кодирующий белок бета-лактамазу, являющийся селективным маркером для трансформации штаммов Е.coli (кодирующая область 5064-4204 н.п. в комплементарной цепи); бактериальный участок инициации репликации типа Col E1 3206-4196 н.п., обеспечивающий репликацию плазмиды в штаммах Е. Coli.2. Plasmid pR752 with a size of 5269 bases (SEQ ID No. 2) containing the following regulatory regions and coding regions: an artificial bacterial operon containing the promoter region of the T5 bacteriophage and a gene encoding the 6His-HMP-NLS-DTox- (Gly-Ser) multi-domain protein 5 -EGF (coding region - 115-2199 n.p.); the bacterial operon bla, encoding beta-lactamase protein, which is a selective marker for the transformation of E. coli strains (coding region 5064-4204 n.a. in the complementary chain); bacterial site of replication initiation type Col E1 3206-4196 n.a. 3. Штамм E.coli - M15 (Rep4, pR752), коллекционный номер ВКПМ В-8356 - продуцент модульного рекомбинантного полипептида.3. Strain E. coli - M15 (Rep4, pR752), collection number VKPM B-8356 - producer of modular recombinant polypeptide.
RU2004100070/13A 2004-01-05 2004-01-05 Recombinant plasmid expressing module polypeptide for delivery of photosensitizer and strain escherichia coli vkpm b-8356 - producer of module polypeptide RU2265055C2 (en)

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RU2004100070/13A RU2265055C2 (en) 2004-01-05 2004-01-05 Recombinant plasmid expressing module polypeptide for delivery of photosensitizer and strain escherichia coli vkpm b-8356 - producer of module polypeptide

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RU2004100070A true RU2004100070A (en) 2005-06-10
RU2265055C2 RU2265055C2 (en) 2005-11-27

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012160450A3 (en) * 2011-05-23 2013-01-17 Contango Partners Group, Inc. Modular transport platform for targeted delivery of therapeutic agents

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2639501C2 (en) * 2015-10-28 2017-12-21 Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук Escherichia coli strain - producer of modular nanoconveyor for delivery of pharmaceutical agents to nuclei of target cells
RU2706698C1 (en) * 2019-02-05 2019-11-20 Федеральное государственное бюджетное учреждение науки Федеральный исследовательский центр "КОМИ научный центр Уральского отделения Российской академии наук" NEW CHLORINE e6 DERIVATIVES, CONTAINING FRAGMENTS OF GALACTOSE

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012160450A3 (en) * 2011-05-23 2013-01-17 Contango Partners Group, Inc. Modular transport platform for targeted delivery of therapeutic agents
CN103957941A (en) * 2011-05-23 2014-07-30 远期合作伙伴集团有限公司 Modular transport platform for targeted delivery of therapeutic agents
CN103957941B (en) * 2011-05-23 2019-04-30 远期合作伙伴集团有限公司 The modular transferring platform of targeted delivery for therapeutic reagent

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