RU2003124489A - P30NE2 RECOMBINANT PLASMID DNA ENCODING THE 181-AMINO ACID N-TERMINAL FRAGMENT OF CLASSIC PIG VIRUS VIRUS E2 GLYCOPROTEIN AND PROVIDING ITS EXCESSLESS E. - Google Patents

P30NE2 RECOMBINANT PLASMID DNA ENCODING THE 181-AMINO ACID N-TERMINAL FRAGMENT OF CLASSIC PIG VIRUS VIRUS E2 GLYCOPROTEIN AND PROVIDING ITS EXCESSLESS E. Download PDF

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RU2003124489A
RU2003124489A RU2003124489/13A RU2003124489A RU2003124489A RU 2003124489 A RU2003124489 A RU 2003124489A RU 2003124489/13 A RU2003124489/13 A RU 2003124489/13A RU 2003124489 A RU2003124489 A RU 2003124489A RU 2003124489 A RU2003124489 A RU 2003124489A
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Russia
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virus
p30ne2
amino acid
glycoprotein
terminal fragment
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RU2003124489/13A
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Russian (ru)
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RU2250263C1 (en
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Владимир Георгиевич Андреев (RU)
Владимир Георгиевич Андреев
Светлана Владимировна Безбородова (RU)
Светлана Владимировна Безбородова
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Федеральное государственное учреждение Всероссийский научно-исследовательский институт защиты животных (ФГУ ВНИИЗЖ) (RU)
Федеральное государственное учреждение Всероссийский научно-исследовательский институт защиты животных (ФГУ ВНИИЗЖ)
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Priority to RU2003124489/13A priority Critical patent/RU2250263C1/en
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Рекомбинантная плазмидная ДНК p30NE2, кодирующая 181-аминокислотный N-концевой фрагмент гликопротеина Е2 вируса классической чумы свиней и обеспечивающая его экспрессию в клетках бактерий E.coli, характеризующаяся тем, что содержит BamHI/Hindlll фрагмент ДНК плазмиды pQE30, включающий промотор/оператор фага Т5, синтетический рибосомсвязывающий сайт, терминатор транскрипции фага лямбда и в качестве генетического маркера ген β-лактамазы, детерминирующий устойчивость трансформированных плазмидой p30NE2 клеток бактерий E.coli к ампициллину, содержит также BamHI/Hindlll-фрагмент, кодирующий 181-аминокислотный N-концевой фрагмент гена гликопротеина Е2 вируса классической чумы свиней.Recombinant plasmid DNA p30NE2 encoding the 181-amino acid N-terminal fragment of E2 glycoprotein of classical swine fever virus and ensuring its expression in E. coli bacterial cells, characterized in that it contains a BamHI / Hindlll DNA fragment of plasmid pQE30, including the promoter / operator of phage T5 a synthetic ribosome binding site, a lambda phage transcription terminator, and a β-lactamase gene, which determines the resistance of ampicillin to E. coli bacteria transformed with p30NE2, as a genetic marker, also contains BamHI / Hindlll fragment encoding the 181-amino acid N-terminal fragment of the classical swine fever virus E2 glycoprotein gene.
RU2003124489/13A 2003-08-05 2003-08-05 RECOMBINANT PLASMID DNA p30NE2, ENCODING 181-AMINO ACID N-TERMINAL FRAGMENT OF GLYCOPROTEIN E2 OF CLASSICAL BOG CHOLERA VIRUS AND PROVIDING EXPRESSION THEREOF IN CELLS RU2250263C1 (en)

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RU2003124489/13A RU2250263C1 (en) 2003-08-05 2003-08-05 RECOMBINANT PLASMID DNA p30NE2, ENCODING 181-AMINO ACID N-TERMINAL FRAGMENT OF GLYCOPROTEIN E2 OF CLASSICAL BOG CHOLERA VIRUS AND PROVIDING EXPRESSION THEREOF IN CELLS

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RU2003124489/13A RU2250263C1 (en) 2003-08-05 2003-08-05 RECOMBINANT PLASMID DNA p30NE2, ENCODING 181-AMINO ACID N-TERMINAL FRAGMENT OF GLYCOPROTEIN E2 OF CLASSICAL BOG CHOLERA VIRUS AND PROVIDING EXPRESSION THEREOF IN CELLS

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RU2250263C1 RU2250263C1 (en) 2005-04-20

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RU2003124489/13A RU2250263C1 (en) 2003-08-05 2003-08-05 RECOMBINANT PLASMID DNA p30NE2, ENCODING 181-AMINO ACID N-TERMINAL FRAGMENT OF GLYCOPROTEIN E2 OF CLASSICAL BOG CHOLERA VIRUS AND PROVIDING EXPRESSION THEREOF IN CELLS

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2134834B1 (en) * 2007-03-07 2013-04-17 GlycoFi, Inc. Production of glycoproteins with modified fucosylation
RU2702716C2 (en) * 2018-04-03 2019-10-09 Федеральное бюджетное учреждение науки "Государственный научный центр вирусологии и биотехнологии "Вектор" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ГНЦ ВБ "Вектор" Роспотребнадзора) RECOMBINANT PLASMID pHis6-flagG-protE, WHICH PROVIDES SYNTHESIS OF A RECOMBINANT CHIMERIC PROTEIN, INCLUDING EPITOPES OF GLYCOPROTEIN E OF TICK-BORNE ENCEPHALITIS VIRUS AND FLAGELLIN G SALMONELLA TYPHII AND USED AS A BASE FOR VACCINE AGAINST TICK-BORNE ENCEPHALITIS VIRUS

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