RU2009126233A - Recombinant plasmid DNA pQe30_PS-CFP2 / TurboYFP_MBP7, coding fragment of human myelin basic protein fused 80-104 IN THE COMPOSITION OF STRUCTURES with yellow and cyan fluorescent protein EPeFRET, STRAIN Escherichia coli BL21 (DE3) - PRODUCER said protein, AND METHOD OF PRODUCING RECOMBINANT PROTEIN EPeFRET - Google Patents
Recombinant plasmid DNA pQe30_PS-CFP2 / TurboYFP_MBP7, coding fragment of human myelin basic protein fused 80-104 IN THE COMPOSITION OF STRUCTURES with yellow and cyan fluorescent protein EPeFRET, STRAIN Escherichia coli BL21 (DE3) - PRODUCER said protein, AND METHOD OF PRODUCING RECOMBINANT PROTEIN EPeFRET Download PDFInfo
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- RU2009126233A RU2009126233A RU2009126233/10A RU2009126233A RU2009126233A RU 2009126233 A RU2009126233 A RU 2009126233A RU 2009126233/10 A RU2009126233/10 A RU 2009126233/10A RU 2009126233 A RU2009126233 A RU 2009126233A RU 2009126233 A RU2009126233 A RU 2009126233A
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Abstract
1. Рекомбинантная плазмидная ДНК pQe30_PS-CFP2/TurboYFP_MBP7 размером 4916 п.о., кодирующей гибридный белок PS-CFP2 и YSP, содержащий пептид основного белка миелина 80-104 состоящей из: !фрагмента ДНК, приведенного на рис.1, содержащего промотор транскрипции Т5 РНК-полимеразы, участок связывания рибосомы; ! фрагмента ДНК плазмиды ген β-лактамазы, детерминирующий устойчивость трансформированных плазмидой pQe30_PS-CFP2/TurboYFP_MBP7 клеток Escherichia coli к ампициллину, в качестве генетического маркера. ! 2. Штамм Escherichia coli BL21(DE3)/pQe30_PS-CFP2/TurboYFP_MBP7 - продуцента гибридного белка EPeFRET, содержащего пептид основного белка миелина 80-104. ! 3. Способ получения белка EPeFRET, включающего культивирование штамма-продуцента Escherichia coli BL21(DE3)/ PS-CFP2/TurboYFP MBP7, разрушение клеток и его очистку методами аффинной и гель-проникающей хроматографии. 1. Recombinant plasmid DNA pQe30_PS-CFP2 / TurboYFP_MBP7, 4916 bp in size, encoding the fusion protein PS-CFP2 and YSP, containing the peptide of the main myelin protein 80-104 consisting of:! The DNA fragment shown in Fig. 1, containing the transcription promoter T5 RNA polymerase, ribosome binding site; ! of the plasmid DNA fragment, the β-lactamase gene, which determines the resistance of ampicillin to Escicichia coli transformed with the plasmid pQe30_PS-CFP2 / TurboYFP_MBP7, as a genetic marker. ! 2. The strain Escherichia coli BL21 (DE3) / pQe30_PS-CFP2 / TurboYFP_MBP7 - producer of the hybrid protein EPeFRET containing the peptide of the main protein myelin 80-104. ! 3. A method of producing an EPeFRET protein, comprising culturing a producer strain of Escherichia coli BL21 (DE3) / PS-CFP2 / TurboYFP MBP7, destroying cells and purifying them by affinity and gel permeation chromatography.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2009126233/10A RU2430161C2 (en) | 2009-07-09 | 2009-07-09 | RECOMBINANT PLASMID DNA pQe30_PS-CFP2/TurboYFP_MBP7, CODING PROTEIN PS-CFP2/TurboYFP_MBP7, STRAIN OF Escherichia coli BL21(DE3)/pQe30_PS-CFP2/TurboYFP_MBP7 - SAID PROTEIN PRODUCENT AND METHOD OF OBTAINING PROTEIN PS-CFP2/TurboYFP_MBP7 |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2009126233/10A RU2430161C2 (en) | 2009-07-09 | 2009-07-09 | RECOMBINANT PLASMID DNA pQe30_PS-CFP2/TurboYFP_MBP7, CODING PROTEIN PS-CFP2/TurboYFP_MBP7, STRAIN OF Escherichia coli BL21(DE3)/pQe30_PS-CFP2/TurboYFP_MBP7 - SAID PROTEIN PRODUCENT AND METHOD OF OBTAINING PROTEIN PS-CFP2/TurboYFP_MBP7 |
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| RU2009126233A true RU2009126233A (en) | 2011-01-20 |
| RU2430161C2 RU2430161C2 (en) | 2011-09-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| RU2009126233/10A RU2430161C2 (en) | 2009-07-09 | 2009-07-09 | RECOMBINANT PLASMID DNA pQe30_PS-CFP2/TurboYFP_MBP7, CODING PROTEIN PS-CFP2/TurboYFP_MBP7, STRAIN OF Escherichia coli BL21(DE3)/pQe30_PS-CFP2/TurboYFP_MBP7 - SAID PROTEIN PRODUCENT AND METHOD OF OBTAINING PROTEIN PS-CFP2/TurboYFP_MBP7 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101778169B1 (en) * | 2012-04-11 | 2017-09-13 | 리폭센 테크놀로지즈 리미티드 | Liposomes containing oligopeptide fragments of myelin basic protein, a pharmaceutical composition and a method for treatment of multiple sclerosis |
| RU2679055C2 (en) * | 2016-12-29 | 2019-02-05 | Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) | RECOMBINANT PLASMID DNA POPTIVEC-MBP84-106-Fc ENCODING CONSTANT FRAGMENT OF HUMAN IMMUNOGLOBULIN FUSED WITH FRAGMENT OF MYELIN BASIC PROTEIN, EUKARYOTIC CELL LINES - PRODUCERS OF SPECIFIED PROTEIN AND METHOD FOR OBTAINING MBP84-106-Fc PROTEIN FOR MULTIPLE SCLEROSIS THERAPY |
| RU2691308C1 (en) * | 2018-08-20 | 2019-06-11 | Федеральное государственное бюджетное научное учреждение Федеральный исследовательский центр "Институт цитологии и генетики Сибирского отделения Российской академии наук" (ИЦиГ СО РАН) | BACTERIAL STRAIN ESCHERICHIA coli/pTdcR-turboYFP, HAVING SENSITIVITY TO TERAHERTZ RADIATION |
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