RU2006140913A -
RECOMBINANT PLASMID DNA pET-hHSP70, ENCODING THE HEAT SHOCK PROTEIN hSP70, AND THE STRAIN E. coli BL21 (DE3) / pET-hHSP70 - PRODUCT HUMAN SHOCK PROTEIN hSP70
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RECOMBINANT PLASMID DNA pET-hHSP70, ENCODING THE HEAT SHOCK PROTEIN hSP70, AND THE STRAIN E. coli BL21 (DE3) / pET-hHSP70 - PRODUCT HUMAN SHOCK PROTEIN hSP70
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RU2006140913A
RU2006140913ARU2006140913/13ARU2006140913ARU2006140913ARU 2006140913 ARU2006140913 ARU 2006140913ARU 2006140913/13 ARU2006140913/13 ARU 2006140913/13ARU 2006140913 ARU2006140913 ARU 2006140913ARU 2006140913 ARU2006140913 ARU 2006140913A
Автономная некоммерческая организация "Институт молекулярной диагностики" (АНО "ИнМоДи")
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Micro-Organisms Or Cultivation Processes Thereof
(AREA)
Preparation Of Compounds By Using Micro-Organisms
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Claims (2)
1. Рекомбинантная плазмидная ДНК pET-hHSP70, кодирующая белок теплового шока человека (hHSP70), имеющая молекулярную массу 4,45 МДа (7240 п.о.), состоящая из NdeI-HindIII-фрагмента плазмиды рЕТ-28а(+), включающего промотор РНК-полимеразы фага Т7, участок инициации репликации (ori) и терминатор транскрипции Т7, HindIII-NdeI-фрагмента гена hHSP70 длиной 1941 п.о., кодирующего hHSP70 с последовательностью аминокислот, представленной в Seq №1, генетический маркер Kan, детерминирующий устойчивость трансформированных плазмидой pET-hHSP70 клеток Е.coli к канамицину, уникальные сайты узнавания рестрикционными эндонуклеазами со следующими координатами: SalI-182, PstI-1056, BamHI-2102 п.о.1. Recombinant plasmid DNA pET-hHSP70 encoding a human heat shock protein (hHSP70), having a molecular weight of 4.45 MDa (7240 bp), consisting of an NdeI-HindIII fragment of plasmid pET-28a (+), including a promoter Phage T7 RNA polymerase, replication initiation site (ori), and T7 transcription terminator, HindIII-NdeI fragment of the 1941 bp hHSP70 gene encoding hHSP70 with the amino acid sequence shown in Seq No. 1, Kan genetic marker that determines the stability of transformed plasmid pET-hHSP70 E. coli cells to kanamycin, unique recognition sites p estriction endonucleases with the following coordinates: SalI-182, PstI-1056, BamHI-2102 bp2. Штамм E.coli BL21(DE3)/pET-hHSP70, содержащий рекомбинантную плазмидную ДНК pET-hHSP70 (по п.1) - продуцент рекомбинантного белка теплового шока человека hHSP70.2. The strain E. coli BL21 (DE3) / pET-hHSP70 containing the recombinant plasmid DNA pET-hHSP70 (according to claim 1) is a producer of the recombinant human heat shock protein hHSP70.
RU2006140913/13A2006-11-212006-11-21STRAIN E coli BL21(DE3)/pET-hHSP70 - PRODUCENT OF PROTEIN OF HEAT SHOCK IN HUMANS HSP70
RU2333956C1
(en)
Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт особо чистых биопрепаратов" Федерального медико-биологического агентства
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